SCIENTIFIC ABSTRACT GRAYEVSKIY, A.I. - GRAYEVSKIY, E.YA.

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• Collection: Scientific Abstracts
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• Document Creation Date: January 3, 2017
• Document Release Date: July 27, 2000
• Sequence Number: 11
• Publication Date: December 31, 1967
• Content Type: SCIENTIFIC ABSTRACT

5(2,3) SOV/20-128-2-24/50
AUTHORS: Razuvayev, G. A., Corresponding Member, AS USSR, Grayevskiy,
A. I.
TITLE: On the Determination of Organoaluminum Compounds by the Indica-
tor Method
PERIODICAL: Doklady Akademii nauk SSSR, 1959, Vol 128, Nr 2, PP 309-311
(USSR)
ABSTRACT: The compounds mentioned in the title are used as catalysts in
the polymerization of ethylene, propylene and other a-olefinee,
generally in the form of diluted solutions (0.1-laA). There is
no quick and simple method of analyzing such solutions, It was
of interest to clarify of what kind is the interaction of the
aluminum alkyls, -aryls, and thair derivatives with indicators
and organic bases. It might be assumed that titration is pos-
sible in the presence of the uaual acidic-alkaline indicators,
The compounds mentioned below were, for this purpose, dissolved
in toluene rid of oxygen, and mixed with solutions of methyl
violet in dichloroethane. It was found that the indicator, by
Card 1/3 addition of Al(C 2H5)3 or ita halogen derivatives, turns from

SOV/20-128-2-24/59
On the Determination of Organcaluminum Compounds by the Indicator Method
violet (alkaline form) into yellow or green (acid fo -
Al(C H ) (supplied by Ye. V. Mitrofanova) and Al C rm~
6 5 3 ( 4H9 3 (sup-
plied by A. F. Popov) had a similar effect on theindicator.
When an excess of any organic base is added to these yellow or
green solutions, they turn violet again. Compounds of type
AlR20R and AlR(OR)2 do not change the color of the indicator.
The neutral properties of these substances can probably be ex-
plained by the screening of the 3P-level of the aluminum by
free electron doublets of the oxygen. The authors titrated the
compounds mentioned in the title with some organic bases:
butyl- and ethyl acetate, dimethyl aniline, ethyl ether,
pyridine, etc while indicators were used. Such indicators were
chosen which are well soluble in organic solvents, and produce
a distinct color change in the equivalent point. As a rule,
they contained amino groups: methyl violet, crystal violet,
gentian violet, etc. Figure 1 shows (a,blvig) the titration
curves of Al(C6H 5)3 and A102H 5)2CI, of AlC2H5C121 of the
Card 2/3 sesquichloride, and of A102H 5)2Brby dimethyl aniline in toluene

SOV/20-128-2-24/59
On the Determination of Organoaluminum Compounds by the Indicator Method
in the presence of =ethyl violet. The results obtained allow
the following conclusions: 1) The said curves remind of those
of the titration of strong acide by strong bases. The aluminum
alkyls and -aryls are rather strong aprotonic acids. 2) Di-
methyl aniline reacts with these acids in a ratio of 1 : 1.
3) Al(C 2H5)2 Br and Al C2H5 C12are very different from A106 H5)3
and Al(C2 H5)2 Br with respect to their strength. 4) Al(C 2H5)2 Br
behaves like a dibasic acid, which circumstance has not yet
been explained satisfactorily. 5) When Al(C 2H1)2Cl and Al C2H5 C~
are present at the same time, they can be determined separately.
Table 1 reveals that there is a parallelism between the results
obtained by the dilution method, and the electrochemical data.
On this basis, the authors put the acids in the following order
with respect to their strength: Al(C 2H5)3 < Al(C2H5)2C'<
Al(C2H5 )2 Br < AlC2H5Cl 2' There are 1 figure, 1 tablep and
2 references.
SUBMITTED: June 6, 1959
Card 3/3

S/190/61/003/001/011/020
B119/1B216
AUTHORS: Smolyan, 1Z. S., Grayevskiy, A. I., Demin, 0. 1., Fuk-in, V. K.,
f.',atveyeva, G. N.
TITLE: Certain rules on polymerization of ethylene on heterogeneous
catalysts
PERIODICAL: Vysokomolekulyarnyye soyedineniya, v. 3, no. 1, 1961, 81-83
TEXTs The authors point out the fact that the catalysts of the type
TiCl4 plus organometallic alkylating agent used for the preparation of low-
pressure polyethylene rapidly lose their high activity in the course of the
reaction, dropping to one sixth of the initial activity within 30 to 40 min.
The present work attempts to find the causes for this drop in activity.
Experiments were carried out on polymerization of polyethylene on catalysts
of the systems TiCl 4 + AlR 3 (Al(C2 11 5)2 Br, Al C2H5 C12, Al(C2H5)2 0C2H5'
Al(C2 H5)3v AIC2H5Cl(OC 2H5) and other compounds). Polyinerization was per-
formed in an autoclave at 600C and a pressure of 4 atm. abs. Individual
Card 1

S11901611003100110111020
Certain rule!~ on polymerization of... B119/B216
catalysts were prepared by inixing the components under argon in a special
thermostat and kept there for use. Catalyst activitv was determined from
the initial polymerization rate and, with the same results, from the
polyethylene yield. It %,:as found that the activity of all the catalyats is
low at the very outset but increases to a maximum within 4 to 5 jain and
then drops to practically zero within another 20 to 30 min. The same effect
was observed on catalysts removed from the argon atmosphere and placed in
the reaction vessel in the absence of ethylene for polymerization. The
authors found that the activity of a catalyst of the type under study de-
pends on the concentration rat~jq of Ti31 and Ti4+ (low initial activity
dud to the sole presence of TO ,, maximum activity on reaching the optimum
T13+ : Ti4+ ratio, followed by decrease with increasini Ti3+ content).
Further experiments showed that the optimum Ti3+ s Ti4 .ratio and thus also
the maximum activity may be maintained constant by careful addition of a
corresponding quantity of okiiizing agent (to reoxidize excess Ti)+).
Air and.0 respectively, -,~,ere used as oxidizing agents. There are 3
2'
figures and 3 non-Soviet-bloc references.

S/081/61/000/024/031/086
B117/B147
AUTHORS: Razuvayev, G. A.1 ~Q~ Demin, 0. 1., Minsker..
K. S., Sukharev, Yu. G.
TITLEs Oxidation of triethyl alumiLum.. and study of the catalytic
properties of the oxidation products
PERIODICAL: Referativnyy zhurnal. Khimiya, no. 24., 1961, 240, abstract
24Zh196 (Tr. po khimii i khim. tekhnol. (Gorlkiy), no. 3,
196o. 373 - 380)
TEXT: The oxidation of solutions of Al(C2H 5)3 (1) and its derivatives in
n-heptane has been studied at various temperatures and concentrations.
Reaction products were analyzed as to their content of peroxide compounds
and their decomposition products. Peroxide compounds with an amount in-
creasing with decreasing concentration of the solution and decreasing re-
action temperature are very unstable. At 200C they decompose in very weak
solutions almost immediately to give oxy derivatives of 1, The following
oxidation pattern of I is proposed:
Card 1/2

S/081/61/000/024/031/o86
Oxidation of triethyl B117/B147
I + 0 2 -->[Al-00+(C2 H031 -~,(C2H5)2 A100C2H5--AIC 2H5(OC2H 5)2 (11);
II + I---~:,M(c 2H 5)2 OC2H5 (111). The polymerizability of II and III in
the case ofoL-olefins was studied on systems of I + II + III + TiCl 4'
Oxidation products of I and of its derivatives are ordinary catalysts of
the Ziegler type but much less reactive. When they are added to I. the
quality of the resulting polymer is not deteriorated, but the catalytic
activity of I and the molecular weight of the polymer are lowered, In
order to eliminate the detrimental effect of the admixture, it is re-
commended that the total concentration of the TiCl /RAI catalyst and the
4 a
ratio of C 2H5 to Ti should be increased at the same t--me. IAbstracter's
note: Complete translation.]
Card 2/2

S/062/62/000/009/003/009
B179/B101 . .
AUTHORS: Razuvayev, G. A., Grayevskiy, A. I., Minsker, K; S., and
Zakharova, V. N.
TITLZ: Synthesis and some'properties of diethoxy aluminum peroxy
cumene
PERIODICAL: Akademiya nauk SSSR. Iz vestiy~. Otdeleniye khimicheakikh
nauk, no. 9, 1962, 1555 - 1559
TEXT: It is sought to synthesize stable aluminum organic peroxide
compounds free from impurities. Three syntheses were*studied: (1) the
react'ion of diethoxy ethyl aluminum with cumene hydrogen peroxide, (~) that
of triethoxy aluminum with cumene.hydrogen peroxide, and (3) that of
diethoxy aluminum chloride with the Na-salt of cumene hydrogen pegoxide.
Reaction (1) takes place only at temperatures higher than 15 - 2 0 C and in
practice is not completed. Reaction (2), occurring at a maximum
temperature of 26 - 30 0C likewise does not complete its course and the
reaction mixture contains no compound with an R-Al bond. Beat results were
obtained for the reaction
Card 1/3

S/062/62/000/009/003/009
Synthesis and come properties ... B179/B101
I ;--~NaCl + A'
Cl-Al(OC H ) + Na-0-0-C H --;?icl-AI(OC H L(OC If
2 5 2 9 11 2 5 2i I' _ 2 5 2
;,Na-61 10-0-C it
1 9
LO-C if
0 9
This took place at 5 C in xylerTe solution. NaC1 precipitated as fine
crystals, the surplus aluminum alcoholates were evaporated and the peroxide
was extracted with ether. Sometimes an amorphous complex compound having
the composition AlCl(OC 11 -NaOOC If was precipitated with the NaCl. The
2 5)2 9 11
peroxide C(C 2H50 2)AlOOC(CH 3)2- C6H is a solid, white, amorphous substance
which melts and decomposes at 113 C; it is easily soluble in xylene,
benzene and chloroform. Its solution in xylene decomposes appreciably
fast even at room temperature. At 900C the decomposition is energetic.
Its -oroduct.9 are dimethyl-phenyl carbinol, acetophenone, a-methyl styrene,
ethyi alcohol and aluminum hydroxide. The polymerization of methyl
methacrylate, styrene, acrylonitrile, vinylidene chloride and vinyl
chloride using (C 2H50)2 AlOOC(Cff 3)2'C6ff5 as radical catalystv gave a good
yield of polymers. In the case of vinyl chloride, the yield of polymer
Card 2/3

j S/062/62/000/009/003/009
Synthesia nnd oome properties ... B179/B101
increased with increasing content of peroxide in the reactions meditim.
The polyvinyl chloride (decomposition temperature up to 1500C, thermo-
stability 6 - 7 min) 'was amorphous and insoluble- in either cyclohexanone
or dichloro ethane, owing to str~ongly branched or net-like structure.
There are 1 figure and I table.
ASSOCIATION: Nauchno-issledovatellskiy institut.k'himii, Gorlkiy*(Scientific
Research Institute of Chemistry, Gorlkiy)
SUBMITTED: blarch 1, 1962
Card 3/3

5/079/62/032/003/007/007
D2011/J302
AuTliuks: Razuvayev, G. it. and Vskivi .1.1.
TlTLE: Synthesis of an organic peroxide of aluminum
Ph~(IOJICAL: ZhUrnal obshchey khimii, v. 32, no. 31 1962, 1006-1007
T!~:U: The peroxide Al(OC,11 5 )2 OOC(C113 ) 13C6H5 was prepared by : (1) the
interaction of Al(OC 11 ).)Cl and NaOOC(C11 ) C 11 , (2) the interaction of
2 5 ~ 3 2 6 5
AVOC 2115)3 and HOOC(C11 3)2 C6H5, with and without a solvent, and (3) by the
interaction of Al(OC 2H 5),C1 with HOOC(CH 3)2CGI'5 in the presence of Na/_1t011.
In the latter case the yicld was loacr than in (1). The compound, a color-
less powder, melted at 113 0C with decomposition, was partially hydrolyzed
in air and completely in acids and was extrei;iely soluble in hydrocarbons.
On heating with iso-PrOH it gave an almost quantitative yield of acetone.
Like Al(OC 2H5)2 OCC 2H5, the compound promoted the polymerization of vinyl
Card 1/2

S/079/62/032/003/007/007
Synthesis of an organic ... D204/D302
chloride and methyl incthacrylate. There are 2 Soviet-bloc references.
SUB,%IIT2F,U: December 13, 1961
Card 2/2 k

ASD pc~- -4 :RWW
m
L IV05- LW VE" 4/Pr
ACCESSION HR'-.
M- RSs Razuvazvv, Go A~ -(Corri -mWier, AN, SSSR) Minaker, K~
' A.,
A
-'Grayevskly, A. 1.
TITIS: Initiating low-tomiarature y2lywrization -f vlgl a With
:Ariothylaluminum by co-oattalytio aotion of oxygen
SOURM AN SSMo Dokladylvi 151P no# 1, 1963P 1l()-113
iTOPIC T"S. low~-temjpqraturq P*17wrizatlonj.,vinyl chlorldpo triathylaluminumi
!oxygen, diethoxyethylaluminum, eyadiotootic macromolecule
'ABS'MLGT: The induotion period of low-4emperature (-3QC) polymerization 6f 1r,
.,chloride with triethylaluminum. and oxygen do-pands on the oxidation of triethyl-a
Aluminum-~-' A study of its 3 oxidation stages indicated high polywrLzation in the
:2nd stage (diotbox~Mthylaluminum) but no or. very, small polymerization In the let
(dietbylaluminum, otboxide) and 3rd (diathox"luminum, paroMthyl) stagose PVO
yield depends on solvent, increasing with solvents in which it is soluble, sege
in dichlorethane or in halobenzons~ yield is 5 time greater than in aliphatia or
aromatic hydrocarbons# in which P70 is not too soluble, In oxygen-oontaining
;solvents PVC yield is lowered: the eloctron-Oonor agents complex with TEA9
Card

L 17o62-63 EPF(c)/EWP(q)/EWT(M)/BDS s[b62/63/ooo/oo4/oo6/022
AFFTC Pr-4 -RM/WW/JD
ADTHORt. -Minsker, K.s., Biryukovs V*I*# Grayevskiy. A.I., and Razuvayev,
G*A,
TITLE: Interaction Between 9.~~num Alkyls and ogen
PERIOMCAL: Akademiya nauk SSSR. Izves ya. Otdolenive khimich%;L nauk,
no. 4, 1963, 637-64o
TEXT: The intera ti f triethylaluminum with hydrogen on Ziegler type
c L3s
.heterogenic complex ca ~Yas studied. In the gaseous phase of the reac-
tion ethane is acci, Wat h the-simultaneous formation of diethylaluminum
h
hydride. A connection is shown for interaction of triethylaluminum and hydrogen
with the effect of molecular hydrogen on the stereospecific po3qmerization of
olefine under the effect of Ziegler catalysts. An analogy was noted in the
activation mechanism of blefin and hydrogen molecules on the Ziegler complex
catalysts. There are 2 figures.
-'June 19, 1962
SUMMED:
Card 1/1

RAZUVAYEV., G.A.; GRAYEVSKIY, A.I.
......
Comple"s formed by aluminum alkyls with alcohols. Zhur.ob.khim.
33 no.7s2423-2424 J1 163. (MIRA 16:8)
(Aliminum organic compounds) (Alcohols)

RAZUVAYEV, G.A.; MINSKER, X.S.; SANGALOV, Yu.A.; GIIATEV9~.X,
Initiation of low temperature polymerization of vinyl chloride
by triethylaluminm aided by the cocatalytic action of oxygen.
Dokl. AN SSSR 151 no.lillO-113 Jl 163. (MIRA 16:9)
1. Chlen-korrespondent AN SSSR (for Razuvayev)
(Ethylene polymers) (Aluminum) iCatalysis)

MINSKER, K.S.; GRAYEVSKIYO A.I.; RAZUVAYEV, G.A.
11~-
Polymerization of methyl wthacrylate in the presence of organo-
aluminum compounds. Izv.AN SSSR.Ser.khim. no.8:1483-1487 Ag
163. (MIRA 16:9)
(Hathaerylic acid) (Polymerization)
(Aluminum organic compounds)

RAZUVAYEV, G.A.; GRAYEVSKIY. A.I.-, MINSKER., K.S.; BELOVA, M.D.
Oxidation of aluminum alkyls. Dokl. AN SSSR 152 no.1-114-116
S 163* (MIRA 16:9)
1. Chlen-korrespondent AN SSSR (for Razuyayev).
(Aluminum organic compounds) (Oxidation)

MINSKER, K.S.; SAJIGALOV, Yu.A.; GRAYEVSKIY. A.I.; RAZIJVAYEV, G.A.
Low-temperature polymerization of vinyl chloride in the presence of the
system orgnnoaluminum compound - oxygen. Vysokom.soed. 6 no.2:269-273
F 164- (MIRA 17:2)

RAZUVAEV.. G.A.; GRAYEVSKIY, A.I.; 1,11INSER, K.S.; SA1.CIALOV, Yu.A.; MALYSH-EVA,
K.M. "
Some regularities in the polymerization of vinyl chloride in the
presence of Ziegler-type catalysts. Vysokom. soed. 7 no.8:1364-
1367 Ag 165. (MIRA 18:9)
1. Nauchno-issledovatellskiy institut khlororpani4cheskikh produktov
i akrilatov.

wALHIM, Tevgeniy GrigorlyevIch; GRALSYMIT, A.M., red.; SYCHKIN, A.K.,
tekhn.red.
[The first step; remats of the first year of the seven-year
plan In Perm Province] Perwyl shag; itogi pervogo goda
gemiletki, w Permskoi oblasti. Perm', Permskoe imishnoe isd-vo,
196o. 46 p. WRA 14:3)
(Perm Province--Agriculture)

PETROV, Ivan Ivanovich; GRAYEVSKIY, A.M., red.; FILIPPOVA, K.G.., tekhn.
red.
(Towards a new upsurge in the agriculture of the western Urals]
K novomu pod"emu sel'skogo khoziaistva Zapadnogo Urala. Perm',
Permskoe knizhnoe izd-vo, 1960. 29 p. (MIRA 14:11
(Ural Mountain regior,-Agriculture)

PLCXHIIISKIY, Aleksey Andreyevich;.. GFLAUVSKIY, A.M., red.; SUKMANOVA,
K.G., takhn.red,
[Industrial equipment of communist tomorrow] Tekhniks kommu-
nistichaskogo zavtra. Perm', Permakoe knizhnoe izd-vog 1960.
6o p. (MIRA 14:2)
(Industrial equipment-Technological innovations)

BAKANOV, L.V.; CjRAYEVSKIY A.P.; LEBEDEV, V.D.; STABNIKOV, M.V.
Determining the composition of matter filling the sensitive layer
of a diffusion chamber. Prib. i tekh. eksp. 9 no.1:197-198 Ja-F
164. - (MIRA 17:4)
1. Fiziko-tekhnicheskly institut AN SSSR.

11777!,57 * r'T
r
rl, 1 1 , ~~ . -, .
E. J. Grayevsky: "Thermonreferendum thermal optimm in f t-ghwat-er -,:ollnr~ks and in
r
arthropoda.11 (P. 455)
SO: Journal o' Cicneral Vol. 7, No. 6, 194-4

GRA--VSKIY, E. Ya.
Gj~A~VSYIY, -. YA. ilAction of Ultre-vl,)I-t -Rayi-, upon the Spor--!s of
Phyc6myces notens At the Temperature of Liquid Air find Fit
Room Te.,Ynperature,lf Cgm7)t,-s R-rid]j~_LD&:LaqXLAe I'Aced-mle
des 5cience� de 1~U-RSS-, vol. 53, 1946, pp. 843-816. 511 P444
SOURCEP: SIRA SI 90-53 15 Dec. 1953

GRAYEVSKri, L. Ya.
IIT~he Action of Ultra -,,--;ole 'u 111,ays at the 'Te-,peratm-e of Liquid Air, and at '100111
I
Te-mperaturc, on the Spores of Flrycormyce3 ldtens," DAIIII SSSIL, 53j, 9, Pp 349-52,
1946.

C-11,YEVSKI'l, E. Ya.
,e -- - .1
IrThe Hadiation-Sensitivity of Protoplasm at the '" mperatiwe of Lin-ld Air
Priroda 11, pp 57-56, 1947 -

GHA)~14M;KIY, S. Ya, and STICELIN, G,1 S.
Mbr., Central X-Ray, Radiological, and C,,jncer Institute, USM Ministry of
Public Fealth, -1947-
"Correlation Between Different Damage Indicators of an Isolated 14uscle
Damaged by IYeezing," Dok. AN, 58, 11o. 2, 1947

PA5/49T97
LIM/PadicivA Protoplasm. My 48
Medicine Cold, Effects
*Life and Low Temperature," E. Ya. Grayevskiy, 121 PP
"Prirodao No 5
Discus3es affect of near-free-il.ng temperatures on
protoplasm, crystalline condition of protoplasm arA
harmful affect of ice, and effect of deep freezing on
protoplasm.
qw 5/49T97

for Du-
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of -!xncr, ai~d "-ul' ~i oho-; Lhz-n bact-r,* wtrt )rl-v -t
-q!.,tn let f,.r-..Ir.I., or is Possibl-c, --dJ rv:-,~ I-v' t'r- r 10 d
,,,ritn tn- objt~t r~!!=Intd :it tl~~ tcmutr-ture, of ail-. Con,-'-d,~z t'-at r-O-isztz~.nct to
'I of bact-r-'a co!"-4,dni, -, consiltrab-t.~! iT!icurit of -r.~t-r il-:: du!! 1c) "1,!ir 5-all
o~ _iu___ ..- 4 - 1, v u 1 1.
c
si,~d) %,hich i-nns-i.-tz dth..,dmt.-!.rn of C-11s x-,.rrouni, n, frc~zts, tI-.tr-,bv
facilitatirll- vitrification o~ protoplaa.,.. Un,-!!.-r caniMion,--,
hol.:tv.!x, cold c--t- kill ftvm re~sislu-,Int
Submittr-d 22 il-in
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coal (:~- K--- ~, ,.- Ir- V'~, ~ .! ~ . i !7- 1 "V--~ .
actift.
S., . 32 XSf-43(101).-Pbolodynarnip ef-
lfectg. e.g. of I-Mys, m;v reviewed with respect to their in-
du icr on crIl diviskm and raher hiol. nd theit
Wf"ili~ity 6, dy" mch at methylelle. red
' I'
22 reftfenc". jullAn F. Smith

GRAYNSKIT, Te.Ta.; OCHINSKATA, G.K.; SHAAK, N.Y.
Nature of the photodymnic process. Sh. obeh. biol., Moskva 13 no.
3:211-231 Maw-June 1952. (CLML 22:4)
1. Laboratory of 1xpertmental. and Pathological Morpholoff of the
Central Roentgenological, Radiological, and Cancer Institute of the
Rinistry of Public Health USSR.

r'm']mxIy'ye.yA.
Relation of photodymmic process to temperature. Dok1a4y Akad. nauk
SSSR 83 no-2:215-217 11 War 1952-- (CLKL 22:1)
1. Presented by Acadmiclan 1. A. Makelmov 2 Janmar7 1952. 2. Central
Roentgenological, Radiological, and Cancer Institute. Leningrad.

jd
T.
P Oeduenic effect in the ultraviolet spectrum. r. Ya.
Rh- k Itornigenot. Radiol. an
%
!I ( , .1 Cancer Inst.,
J~;Mlg'3 of I le:111131 DoOwly Akad. Xisnkr,~~
S.S.S~R. 83,- 303-ZO92;.-T. det.. the bactericidul Cf--
feet of the ultravioleftivs in the presence ofabsnr4ers a no.
-y Blue 11. cosin.
of dyes, metbylene blim; New Methylene Sk N
erythrosen, acridiftc ced and orange. and tMaflavinc, were
_added to cultures of Fscherkhia coli find Paramecium cav-
No iticreased effect was noticed. The effectiveness
dalian.
of the dyes Is apparently, limited to radiation in the visible
range. The 24-hr.-old cultures-wgre dild. with a Vhysfol.
soln., and the dye was added to make a concn. of 0.005-
0.0W25%- After 30 min. the 61tures'vkere placed on
slides and exposed to radiation for 6-30 min. 15 m(emncts.

GRAYMKIY, Ye. Ya.
Beta-rays sensitization of bacteria to the action of high and
low temperature and to repeated effect of beta-raye. Doklady
Akad. neuk SSSR 83 no.4:565-568 I Apr 1952, (CLML 22:2)
1, Presented by Academician A. I* Oparin 31 January 1952.

GRANIVSKIT, N.Ta. (Moscow)
~ - --
, -11,
, r,
Biological iffect of ionizing radiation. Usp.soyr.bio. 37 no.2:
158-176 Mr-Ap 154. MIA 70)
(Radiation-Physiological effect) (Ionization)
M - 4-7. 6 J a. 4-4'- 1 OAUJArA

USSR/Humn and i-nimal PehysioloMr - Internal Sceretion. T-7
Hypaphysis.
Abs Jour : Ref Zhur - Biole, No 18, 1958, 84339
Author : Graycvskiy, E,Ya., Neyfal-di, A..A.
Inst L$ USSR
Title The 11ole of Hypophysis in RVaiments of Amphibian Ova
Cause-t ')y General Ionizin~; Irradiation.
Orig -Pub Dokl. AN SSSR., 1956~ 111, 110 5, 11o4-i1o6
Abstract Frogs (F) were irradiateci with Cpma rays for 19 hours
(7,000 r), and then kqpt in a teuperature of 16-170 C.7
for 14 dap* In another test, irradiation doses o4f 10,000
r were used, and after irradiation the animals were kept
at the above rientioacd tenparature for 21-30 days. Then,
F were killed and their hypophysis was inserted into the
lymphatic sac of femile fro~:s, which have been kept at a
Vard 1/2

%A-nnv;:v>n%,t, le-- T&.

e studies on the protection of anunal OrgAnLXM agalnut LUO
harmful action of ionizing radistions
D
.

4
'&ssiya Akad. Nauk S.S.S.R. 00 Himema
4
~
'1
Y'C
V
Almn*Mergii 1955, Zaiedaniya OMd. Birl.
ak
4 1
3
)
I
-Tests of various substances were made with :Wce sub-
jected to 700 r. of x-radiation or higher lethal dose being
~Wr,), A protective prophylaxis was 0 taintd with CO,
i
A
dl
h
Co
h
i
l
d
j
d
-
nt.
i
enam
ero
ur, a
rau
ne, an
n, morp
s.
p
therapeutic protective action resulted front intravenous In-
Jeeti=3 of bone marrow homogtnate and to a lesser extent

)l of nudeoprotelus derived fror~ edl nueld, as well as dill.
N
HCO
h
CO
N
OH
d
h
b
ff
h
&
ate
ers,
w"t
emost
a
s, an
u
osp
,
p
Itiliective agent found In the Ist group, bone marrow In the
2nd gmp. The protective action IM11 LL histological View-
point Is to some extent a dela
in
twtion for the dA
e
na
r
y
g
-
g
'
i
l
I
on
and accelerat
ve; act
n the regeneration cycle.
cycIc,
Tin: protective action of CO Is that of gr,leral reduction or
n of the radiation effects. 61 references,
U. bt, Kosolap
-of
A""q,
EM

G R-R REVS K.ly,-
USM/Biology' Experimental morphology
Card Pub. 22 - 23/54
Atthors I Graevskiy, E. Ya.'* and Korchak,, L. I.
Title I content Of .0AL1fW%71 groups in muscular tiesue in the normal state
and after applioation.of X-rays in fatal doses
Periodical I Dok. AN. SSSR 102/5, 939-941f, hne 21p 1955.
Abstract I Experiments are described which were conducted to determine the effect
of ionizing radiation, in fatal aoseaj on the content of sulfhyd4l .
groups in muscular tiosues of animals. The experiments were conducted
with white mice exposed to X-rays of 700 r(roentgen). The content of
sulfhydryl groups were neasured in the brainsp kidneys) liverj, lungs,
mid spleen of normalanimals and.animals subjected to X-rays. Twelve refer-.
ences: 1 French, 2 USSRp3 USA# 3 Germ, and 3 Brit. (1947-1953). Table.
331stitution The Acad. of So., USSRO A. N. Severtsov Institute of Animal Morphology
?resented by Academician A. L Oparin, February 17, 1955

USSR / General Biology. Physical and Chmical Biology. B-1
Abs Jour t Ref Zhur - Biol., No 2, 1968, No 4738
Author : Gra vskiy, Z. Ya., ZinovIeva, I.G.
Inst a Not given
Title : The Effect of Small Quantities of :E)nizing Radiation on Pa-
ramaecium Caudatum (on Radio-Stimulation).
Orig Pub t Dokl. AN SSSR, 1956, 110, No 3, 379-382
Abstract : The effect of small doses (0.005, 0.05, 0.5, 2.5 and 10
rep per hour) of radioactive emanations was studied on the
rate of Paramaeoium osudatum division. The mdiation Wqs
conduot9d uninterruptedly fbr 7 days. B35 (in the form of
N&2 S3 504) at a dos* of 0.02 rep per hour and 1131 (in the
form of NAI131) at doses of 0.05 - 5 rep per hour yielded
Card 3 1/2

_qAA 9 - SHAPIRO. Nikolay Iosifovich-, f
, vf~kj~ & i
~Hli wor zdatelOstva; NICHIPORDVICH. A.A.,
otvotetvennyy redaktor; ASTAFITZVA, G.A., tokhnicbeskiy
rodaktor
(Present-day problems in radiobiology.] Sovremenzwe voprovy
radlobtalogii. Hooky&, Izd-vo Akad.nauk SSSR, 1957. 93 P.
(KLRA NO)
(Radiobiology)

20-2-15160
On the Absence of the Protective Influence of the Ifistotoxic Hypoxia Under
the Action of an Ionizing Radiation
is shortly discussed. Previous tests showed the followinz:
Neither carbon monoxide nor potassium cyanide in the concen-
trations and durations of tests occurrin,- here essentially
changed the surviving quota of the bacteria and their capa-
bility of propagation. The results of these radiation-tests
in bacteria are compiled in a table. In their action upon
microorganisms before and during the radiation neither carbon
monoxide nor potassium cyanide exerted a favorable influence
upon the capability of propagation of the radiated bacteria.
In most cases even a tendency toward intensifictttion of the
radiation-dama~ze was noticed in the presence of potassium
cyanide and carbon monoxide. Thus the oppression of the re-
spiration-enzyme-systems does not change the sensitivity
to radiation of the bacteria Escherichia coli. The authors
further investigated the influence of potassium cyanide upon
the radiation-damage of mammals (white mice). The introduction
of potassium cyanide in almost lethal doses does not cause
any essential increase in the survivin,,~ quota and no marked
Card 2/3 prolon.-ation of the lives of the radiated animals. This is

W-7 1C11V V/ 4- V
_-j2H(31RS: Grayevskiy, E. Ya., Korchak, L. I. 2o-4-19/6o
TTTLE. The Influence of X-Radiation on the Distribution of Dyestuffs
Intravenously Introduced in Mice Tissues (Vliyar;iye rentgenovs-
kogo izlucheniya na raspredeleniye v tkanakh myshey vnutriven-
no vvedennykh krasiteley).
PERIODICAL: Doklady Akad.Nauk SSSR, 1957, Vol- 115, Nr 4, PP. 7o2 - 705 (USSR)
ABSTRACT: At first reference is made to 16 relevant earlier works. The pre-
sent paper shall determine how the distribution of substances in-
troduced into the organism changes under the influence of radia-
tion and by what the changes are determined. The test was made
with 6-8 weeks old white mice of both sexes with a weight of
from 18 to 22 g. The entire animals were once irradiated with
X-rays (dose 40, loo and 5000 r with a dose power of 47 -84
r/min)- In the first series of tests the distribution of a neut-
ral red dyestuff and of methylene blue in the organs of normal
and irradiated mice was investigated. These dyestuffs were intra-
venously introduced at different times (2 and 0' hours, 1 and 3
days). 6o minutes after the introduction of the dyestuff the ani-
mals were beheaded and the dyestuff extracted from liver, spleen,
brain, lungs, kidneys and intestines. The data thus obtained are
expressed in percents of the dyestuffs accumulated in the corre-
Card 1/2 aponding organs of the non-irradiated control animals and compil-

AUTHORS: Grayevskiy, B. Ya., Zinov'yeva, Ye. G. 2o-3-16/59
TITLE: On the Problem of the Possibility of Changing the Radio-
Sensitivity of a Call by Means of Fluorochromes (K voprosu
o vozmozhnosti izmeneniya radiochuvotvitellnosti kletki pri
pomoshchi fluorokhromov)
PERIODICAL: Doklady AN SSSR, Vol. 118, Nr 3, Pp. 476-478 (USSR)
ABSTRACT: The seneitation of biological objects for ionizing radiation
by means of fluorochromes would be of considerable interest
for radio-therapeutics. Besides the finding of a correlation
between the radio sensitivity of the cells and the
hematoporphyrine, which is contained in them, would render
possible, the explanation of the mechanism of the initial
reaction, which takes place under the influence of the
radiation. In this connection the authors wanted to explpain,
how far this dye-like substance can change the radio
sensitivity of a cell. The effect of the following dyes w:~s
investigated: Hematoporphyrine (0,001 - 0,005 %), tiypa-
flavine (0 00092 - 0,0001 %), and fluorescine (0,00005 -
Card 1/3 - 0,0002 %~- 300 parametia. (parametsiya) in a 0,3 ml non-

'On the Problem of thc, Possibility of Changing the Radio- 2o-3-16/59
Sensitivity of a Cell by Means of Fluorochromes
peptoneous medium(which contained the dye in one of the here
given concentrations) and control-parametia of the same
quantity and in the same medium, but without dye (control 1)
subjected in plexiglan cylinders to an action of X-rays
(dose 100 kiloroentgen) once and at the same time. Also the
other conditions of the irradiation are Given. As second
control for the darkness effect of the dye (control II)
parametia, were used, which were submerged for 60 minutes in
the highest concentrations of the dye and which were not
irradiated. The authors observed the velocity of cell
division and the rate of survival of the animals. The results
of these experiments are compiled in a table. An ionizing
radiation of 100 kiloroentgen noticeably suppresses the
tempo of the division of the infusoria'on the first day after
the action of the radiation. But the preparation velocity
was normalized completely already on tite second day. The here
applied fluorescence-materials did not sensitize the
infusoria aFainst the Roentgen radiation. The dyes under the
influence of 100 kiloroent-en did not become toxic either
and the sensitivity of the irradiated parametia against the
Card 2/3 colours does not change noticeably in this case. The photo-

On the Problem of the Posoibility of ChanCin- thr Radio- 2o-3-16/59
I U
ISensitivity of a Cell by Means of FluorochromeB
dynamic effect is not very probable in case of irradiation
with X-rays. Accordin- to the results found here the
U
increase of the radio sensitivity of the organisms and
tissues in the presence of fluorochroimeu is to be explained
obviously by the aummation of the effect of the ionizin,-,
radiations and of the chenical or photodynamical effect of
th colour or of the pigment. There are 1 table and 9 re-
ferences, 6 of which are Slavic.
ASSOCIATION: Institute for Morpholoay of Animals imeni A. N. Saverteov
AV USSR (Inztitut morfologii zhivotnyth imeni A. V.
Severtsova Akademii nank SSSR)
PRESENTED: AuGust 8, 1957, by A. I. Oparin, Member of the Academy
SUBMITTED: August 2, lg,--7
AVAILABLE: Library of Congress
Card 3/3

GRA,YEVSICLY, 1. Ya.
"Investigation ct Local and Delayed ifffects of Ionizing Radiation."
paper to be presented at 2nd UN Intl.' Conf. on the preaceful uses of Atomic
Energy, Geneva, 1 - 13 Sept 58-

210)
AUTHORS: Grayevskiy, E. Ya., Zinov'yeva, Ye. G. SOV/2o-121-5-19/50
TITLE: An Investigation of the Radiosensitivity of a Cell in a
Repeated Influence of Ionizing Radiation (Issledovaniye
radiochuvstvitellnosti kletki pri povtornykh vozdeyst-
viyakh ioniziruyushchey radiatsii)
PERIODICAL: Doklady Akademii nauk SSSR, 1958, Vol 121, Nr 5,
PP 837 - 840 (USSR)
ABSTRACT: The authors investigated the ability of unicellular
organisms to heal radiation damage and to adapt them-
selves to such affections. These experi-menis were
carried out on Paramecium caudatum, the method of the
investigations was discussed in previous papers (Refs lt2),
The variations of the rate of cell fission and of the
percentage of the surviving organisms of Paramecium
caudatum after single and repeated irradiations were
used as criteria. The unicellular organisms were irradiated
by a dose of 100 000 r at a temperature of OOC. After such
Card 1 an irradiation, all the 6rganisms continued to live if

An Investigation of the Radiosensitivity of a Cell in a S07/2o-121-5-19/50
Repeated Influence of Ionizing Radiation
transplanted into a non-irradiated medium. But the rate
of the cell fission is diminished by 80% on the first
day after the irradiation. After 2-3 days, the fission
velocity is restored to its initial value. The variations
of the radiosensitivity of the Paramecia under the
influence of radiation were investigated by repeated
irradiation of the same dose (100 000r). There were
various intervals between the initial and the repeated
irradiations. After repeated irradiation of the parameciae
by 100 OOOr (in intervals of 3 and 6 hours, total dose
200 kr) the death rate amounted to 93 and 87%, respectively.
But the slowing down of the fission velocity was not
noticeably different from the parameciae whic'.1i were
irradiated only once. The authors then investigated the
radiosensitivity for the case that the total dose of
radiation is gradually increased. The decrease of the
fission velocity did not depend on the total dose, it
was caused by the immediately received dose. The survival
Card 2/4 rate of the infusories depends on the manner of irradiation

An. Inverti-ration of tne Radiosens-itivity of a Cc,11' in. aS-~7/2o-121-5-19/50
Rezeated !~fluence of IonizinG Radiation
in a more complicated way. As a rule, the survival rate
was not chanCed by repeated irradiations. But in some
cases very distinct periods of especially high sensitivity
,aere observed or the back6round of tile monotonous reactions
~ , . According to t'liese
caused by any repeated irradia t4on
results, t1fte veE~etactive fuixtions affected by the
radiation are quickly and practically totally restored
if the cell is transplanted into normal conditions. There
are 2 tables and 3 references, ahich are Soviet.
ASSOCIATION: Institut morfologii zhivot-n.ykh -Jm.A.N.Severtsova Akademii
nauk SSSR (Institute of Animal Mlorpholoey imeni A-71.
Severtsov,AS USSR)
FRESETITED: Ma., 25, 1958, by A.I.O.)--rin, Academician
SUB','TTTED: April 19, 1958
I'L If
C,7.j-d '/ 4

21(-') SOV/2o-12?-3-16/57
., j
ATITHOR3 Grayevskiy, B. Ya., Konutantinova, M. M.
TITLE: On the Antiradiat'WPXQt9 tive Effect.of Subotances Tilockinj
the Transport oflox (0 protivoluchevor. -ashchittnnr.
deyotvii veshchestv, bloiciruyushchikh transport kisloroda
,romoglobinom)
PERIODICAL: Doklady Alrademii nauk SSSR, 11058P Vol 122, Nr 39 PP 381-364
(USSR)
ABSTRACT: The authors made a rough comparison of the variations of the
accumulations of inactivated hemoglobin in blood with the pro-
tective effect at various instants of time after the be.ginning
of the influence of the protective substances. The experiments
were carried out on grown-up male and female white nice (wei-lit
0 U
20 - 25 g, age 0 - 12 weeks). T~e animals Yrere totally irra-
diated by a dose of 900 r of Co 0 y-radiation. The dose rate
amounted to 600 r/min. The authors inveatigated'the inqctiva-
tion ard the restoration of heno--lobin at various instants of
time after the introduction of sodium nitrite into the or,-an-i--.
or after placinr the animals into an atmoophere containing;
Card 1/3 carbon monoxide. "'lie survivnI rate of the irradiatQd aninals

SOV/20-12,--3-16/157
On the Antiradiation Protective Effect of Substances Plockin.-I the TransLort
of HemoGlobin
was determined for a time of 30 days. The amount of mathemoglo-
bin distinctly grows already 5 minutes after the introducti~,-l
of sodium nitritep and it reaches its maximum value (65 5)
40-60 minutes after the introduction of sodium nitrite. The
content of methemoClobin maintains this value for 1 - 1,5
~,ours. A distinct protective effect of sodium nitrite cannot
be o~,served before 20 minutes after its introduction into the
organism. The maximum of the protective effect was observed
40 - 60 minutes after the introdution. The period of the
maximum intensity of the protective action corresponds to the
period of the highest content of methemoTlobin in the blood.
In the experiments with carbon monoxide, the coincidence of
the curves of the hemo,globin inactivation and of the survival
rate was still better. A connection between the quantity of
the inactivat(.d hemoglobin and the intensity of the protective
effect was found. The protection due to carbon monoxide is
more efficient. The following conclusion may be drawn from
the data discussed in this paper: The protective effect of
the prophylactic introduction of sodium nitrite or carbon
monoxide before and during the irradiation is caused by the
Card 2/3 hypoxis. duo to the suppression of the oxygen transport. There

SO V120-1 22-3 -11 r,.. '5-- 7
On the Antiradiation Protective Effect of Substances Blockin..- the Trans~jort
of Hemoglobin
are 2 figuros and 14 references, 5 of which are Soviet.
ASSOCIATION: Institut morfologii zhivotnykh im. A. N. Severtsova Akaderii
nauk SSSR
(Institute of the Morphology of 'Mimals hnieni A. N. Severtnov,
Academy of Sciences,USSR)
PRESENTED: May 22, 1958, by A. I. Oparin, Academician
SUBMITTED: May 15, 195B
Card 3/3

SMITSKIY,A.N.; otv.red.; 44tj1ff3KIYLX.Yn,,, red.; ORIOORlYNT.T.A., red.;
TAL153M.T.G.. red.; ZHARMIT,I.B.. red.; LIOZM,L.D., red.;
MITSKffICH,M.S., red.; FRIMSMYR.A.7a., red.; KHRUSHCHOT.G.K.,
red.; CHENTSOY.Tu.S., red.; SMIRNOY.Z., red.; LAIRM"YWA,G..
tekhn.red.
[Transactions of the Second Histological Conference; plastic and
restorative processes] Plesticheskis i voestanovitellnye protees-
my; truo Ttoroi gistologicheskol konferentaii. Moskva, Hook.
nauchn.ob-vo anstomov, gistologov i enbriologov, 1959. 319 P.
(mIRA 14:5)
1. Kefedra gistologii Mookovskogo gosudarstvennogo universitsta
lm.N.T.Lomonosovs, Moskva (for Stiiditskiy). 2. Laborstorlys radio-
biologii Institute morfologii zhivotnykh Im.A.N.Severtseve AN SWR,
Moskva (for Grayevskly, Zberskiy) 3. Kafedra gistologii, i embrio-
logil leningradskogo seniterno-gigiyanicheakago meditainskogo,in-
st1tuta, Leningrad (for Grigorlyev). 4. 19fedrB gistologii i emb-
riologii 1-go Meditainskogo inatituta im.Sechenove, Moskva (for
Teliseyev). 5. Gruppa biokhimil klatochnykh struktur Institute mor-
fologii zhivotnykh im.A.N.Severteova AN SSSR, Moskva (for Zbarskiy).
6. Laborstorlys rosta i razvitiya Institute eksperimentallnoy bio-
logli ANN SSSR, Moskva (for Liomer). 7. TSentrallneys neuchno-
issledovatellskays Laboratoriya'2-go Mookovskogo meditainskogo in-
et1tuta im.N.I.Pirogove, Moskva, (for Khrushchov).
(HISTOLOGT--CONGRESMS)

HIM
10 1*
IV' I
Piv-
;s A va .4

Ali

GRAyxvsKIT, I.Ta.; KOWHAK, L.I.
Factors which weaken the harmful action of ionixtug
radiations In mammals. Trudy Inst.morf.zhiv. no.24:
5-27 '59. (KIRA 130)
(Z rays-4 ft siological effect)
(Radiation protection)

GRAYRVSKIY. 1. Ya. - KORCHAK. L. 1.
Distribution of dyes intravenously injected in the tissues
of normal and X-rayed mice. Trudy Inst.morf.zbiv. no.24:
28-37 '59. (HMI 130)
(STAINS AND STAINING (MIGROSCOPr))
(Z RArS-PIffSIOLOGICAL BMGT)

GRATIVSKIT, E.Ta.; ZINDVITVA. Ts.G.
Effect of small doses of ionizing radiation on the sur-
vival and rate of division of Paramecium caudatum. Trudy
Inst.morf.shiv. w.24:160-171 '59. (MIRA 13:3)
(Radioactivity--PbVniological effect)
(Paramecium)

GRAYBVSKIY, 3.Ya.; SHAPIRO. I.M. (14oBkva)
-------- 6'eTI-11 Lstruction and repair following injury of the organism by
ionizing radiations. Usp. sovr. biol. 47 no.2.-185-203 Mr-Ap '59.
(RABUTIONS, eff. (KM 12:7)
cell destruction & post-irradiation regen., review (Rus))
(RIGENIPATION,
post-irradiation, review (Rha))

24(0) ~011/20-59-124-2-56/71
AUTHORS: Brodskiy, V. Ya., Grayevskiy, E. Ya., Suyetina, I. A.
TITLE: On the Ways of Action of the Ionizing Radiation on the Content of
Free Nucleotides and Nucleosides in the Bone Marrow Cells '
(0 putyakh vliyaniya ionlziruyushchey radiatsii na soderzhanije
svobodnykh nukleotidov i nukleozidov v kletkakh kostnogo mozga)
PERIODICAL. Doklady Akademii nauk SSSR, 1959, Vol 124, Nr 2, PP 440-443 (USSR)
ABSTRACT: Nucleic compounds are early and easily depolymerized in the organ-
ism if radiation reaction develops. In vitro relatively small doses
of irradiation are sufficient (Refs 4,8,10). The synthesis of
nucleic acids is disturbed in directly irradiated as well as in
screened body parts (Refs 11-13)- Several investigations (Refs 14-16)
have shown that the damage of the cells due to irradiation is not
directly connected with the preceding change of the amount of
nucleic acids. It had-to be determined,whether the changes of the
content of free nucleotides is due to local irradiation effects
or to remote action. Experiments were carried out with white mice
of both sexes. They were irradiated with 700 r X-rays. The follow-
ing variants were applied: 1) total irradiation; 2) irradiation of
the right part of the body; 3) irradiation of one back extremity;
Card 1/3 4) screening of both back extremities with lead plates of a

SOV/20-59-124-2-56/71
On the 'Ways of Action of the Ionizing Radiation on the Content of Free Nucleo-
tides and Nucleosides in the Bone Marrow Cells
thickness of 3 mm. The mice -were killed 6 hours after the irradia-
tion. The amount of nucleotides in the nuclei of the myeloblasts
and neutrophileB in the bone,marrow of the irradiated and screened
extremity was determined by means of ultraviolet cytophotometry,
Figure 1 and 2 show the measurement results of the optical density
(of the proportional concentration) of free nucleotides in the
myeloblast nuclei of normal and irradiated animals. In the,case of
total irradiation the average optical density of the acid-soluble
fraction decreases by five times approximately. Unexpectedly, the
optical density is clearly yeduced (by about 50 %) also in the
screened extremities, irrespective of the size of the surface which
was screened. From the experimental results the authors draw the
conclusion that the remote action of irradiation exerts almost the
same effect on the amount of nucleotides. A somewhat stronger effect
at direct irradiation as compared to the screened bone marrow may
be due to the fact that the factors causing the remote action had
been formed in the immediate vicinity of the parts investigated.
The problem of the connection between the dynamics of the change
of the amount of nucleotides, a premature destruction of the cells
Card 213 in the irradiation-sensitive systems, and the suppression of their

SO11120-59-1 Z4-2-56/71
On the Ways of-Action of the Ionizing Radiation on the Content of Free Nucleo-
tides and Nucleosides in the Bone Marrow Cells
mitotic activity in the irradiated organism has hither.to not been
.solved. The reduction:,of the eontent of free nucleotides and nucleo-
sides is due to remote action. The processes of destruction are
totally, the disturbance of cell division is largely caused by the
local action of irradiation (Ref,18).-There are 3 figures and
18 references, 9 of which are Soviet.
ASSOCIATION: -Institut morfologii zhivotnykh im.,A.-No'Severteova Akademii nauk
.SSSR-(Institute of Animal Morphology impni A. N. Severtsov of the
Academy of Science s,, USSR)
PRESENTED: September 19, 1958, by A. I.,.-Oparin,,Academician
SUBMITTED: June 26, 1958
.Card 313

KONST.kNTINDVA, M.M.; GRAYUSKIY, X.Ya.
Tissular hypoxia as a mechanism of the pyotective action of
adranalinei heroin, and morphine against radiation., Dokl.AN
SSSR 132 no.6:14Z7-1430 Je 160. (MINA 13:6)
1. Inatitut morfologii zhivotmykh im. A.H. Severtsova.
Predstavleno skademikom, A91.0parirWmo
(RADIATION PROTIGTION) (ASOMIA)

82526
S102016011331041301031
11~ 7a 10 a B016/BO67
AUTHORS: Grayevskiyq Ya., Konstantinova, M. M.
TITLE: Study of the Mechanism of the Radioprotective Action of
Some Sulfur Containing Substances -17
PERIODICAL: Doklady Akademii nauk 355R, 19609 Vol. 1339 No- 4Y
pp. 969-972
TEXT: The authors present five hypotheses on the radioprotective effect
of some sulfhydryls and some thiurcnium compounde which are structurally
related with them. These substances are one of the most effective radio-
protective substances. The data of publications do not allow the esta blish-
ment of a connection between the radiation resistiNity of an organism and
the state of its regulating systems. The protective effect has approximate-
ly the same degree in biological objects at different stages of development.
The authors mention further inadequate explanations of the protective ef-
fect (Refs. 11, 15). The assumption that the protective effect of sulfur
containing compounds is due to the so-called "oxygen effect" (Refs. 10,
16-25) is the mostu probable. In conclusiong the authors state that the
Card 1/4

82526
Study of the Mechanism of the Radioprotective S/02(bO/133/04/30/031
Action of Some Sulfur Containing Substances B016/Bo67
hypothesis according to which the protective affect of the substance con-
cerned is based on the anoxia in the organism, that it is also based on t_r
rather contradictory dataq and that it must not be regarded as proved.
The authors wanted to explain the role of the level of molecular oxygen in
tissues for the protective effect. For this purpose they used grown-up
white mice. The above-mentioned effect and the oxygen tension in the liver
and the spleen were studied at different periods after subcutaneous injec-
tion of the following compounds: oysteamin, oystamin, Br.HBr-2-aminosthyl
isothiuronium (AET), Br.HBr-2-amino--5--isothiuronium-methyl-thiazolin
(ARIT), furthermorep HC1.oysteine and SH glutgthione. The animals were
once totally irradiated with gamma rays of Cc 0 (dose: 900r; dose intensi-
ty! 378 r/min, for 2 min 21 seo).They were irradiated 15? 309 60, 1209
and 160 min after the injection. The duration of life was observed within
a period of 30 days. Table 1 shows the number of experimental animals.
Fig. 1 shows the results obtained with eysteamin. This substance has a
considerable protective effect; it does,, however, not reduce the 02 content,
but increases it in the spleen. Fig. 2 shows that also eystamin does not
reduce the oxygen tension during the duration of the protective affect.
2

82526
Study of the Mechanism of the Radioproteotive S/020/60/133/04/30/031
Action of Some Sulfur Containing Substances' B016/BO67
In introducing AET 15 min before the irradiation the protective effect
becomes distinctly marked (Fig. 3 A). The 0 2 content is only slightly
reduced. Analogously, AIMT has 'a weaker effect on the 0 2 level; its pro-
tective effect is also much weaker and only of a short duration (Fig. 3 B).
Gyatein (Fig. 4) and SH gluthathione tension hardly change the 0 2 in both
organs, they have, however, a considerable protective effect. From these
results the authors draw the conclusion that the protective effect is not
caused by tissue hypoxia. At the same time it is assumed that the Gffect
of the above-mentioned protective substances is, nevertheless, connected
with the "oxygen effeat". Hence, it may be assumed that this effect
is not necessarily connected with the radiolysis of water. Perhaps, it
may be explained by the formation of potential disturbances in biological
objects, which may take place only during the oxidation by molecular oxy-
gen. In this caseq the protective effect of the substances under considera-
tion can be related to their capability of preventing the oxidation by
molecular oxygen of thellstructures" damaged by radiation. The authors
thank V. M. Fedoseyev for the synthesis of AET and ART. There are 4 fig-
ures, I tablev and 27 references: 7 Sovieto 1 US, 3 Intern. Conf., and
Card 3/4

82526
Study of the Mechanism of the Radioprotective
Action of Some Sulfur Containing Substances
1 German.
S/020/60/133/04/30/031
B016/BO67
ASSOCIATION: Institut morfologii zhivotnykh im. A. N. Severtsova
Akademii nauk SSSR
(Institute of Animal Morpholo~j imeni A. N. Severtsov of
the Academy of Scienceeg USSR)
PRESENTED: April 4, 19609 by A. I. Oparin, Academician
SUBMITTED: April 7, 1960
Card 4/4

GRAYEVSKIY, E.Ya.; KONSTANTINOVA, MA
Radiation protective effect of some agents and the 'foxygen effect.0
Radiobiologiia 1 no.2;270-277 161. (KIRA 14:7)
1. Institut morfologi:L zMvotnvkh imeni, A.N.Severtsova AN SSSR,
Moskva. (RADIATION PROTECTION) (CMYG24--PHYSIOLOGICAL EFFECT)

30366
S/205/ 1 001/005/001/005
D299/D3o4
AUTHORSs E.Ya. Grayevskiy, and M.M. Konstantinova
TITLE: A study of the mechanism of the protective action of ami-
noeth,yl-isothiuronium on rats irradiated in a state of
hypothermy
PERIODICALs Radiobiologiya, v. 1, no. 5, 1961, 650 - 652
TEXT: The lack of defensive action from cysteine after irradia-
tion in animals with a normal metabolism and its presence in chilled hy-
bernating animals tends to indicate that protective compounds, if at all
capable of weakening -the radiation reaction when introduced after irradi-
ation, can only do this when the development of the radiation reaction is
strongly inhibited. To check this assumption a study was made of the pro-
tective effect of aminoethyl-isothiuronium (AET) Br.HBr. on animals irra-
diated in varying states of hypothermy. The experiments were conducted
with white mice exposed to single gamma-radiation from a co60 source in
a dose of 900 r (LD 100/15 ) at an intensity of 320 r/min. The body temper-
Card 1/3 W

30366
S/205/61/001/005/001/005
A study of the mechanism of ... D299/D3o4
ature of the mice at the time of irradiation in the three test groups
was 379 18, and 60 C. The protective agent was injected subcutaneously
at 9-10 mg per mouse in 0.05 ml of distilled water 15 min before irrad-
iation or 10 - 20 sec after it. The number of animals that survived for
more than 30 days after irradiation and the life span of those animals
which died beyond this period was taken as the criterion of the effect-
iveneas of AET. The results showed that prophylactic injection of AET
into mice with a body temperature of 370 C gave a marked increase in
the survival rate of the irradiated animals and increased the average
life of the animals which died. The propylactic effect was lower in mice
with a body temperature of 180 C. At 60 C no protective action was not-
ed, due perhaps to disturbance of the resorption and admission of the
AET to the radiation-sensitive systems or to inhibition of its conver-
sions. The AET was ineffective in all cases when introduced after irra-
diation. The authors' findings conform to those of D.E. Smith (Ref. 5:
Radiation Res., 12, 79, 1960) who found that the administration of cy-
steine after irradiation to animals of the genus Citellus irradiated in
a state of hibernation with a body temperature of 50 C had no protective
Card 2/3

30366
S/205/61/001/005/001/005
A study of the mechanism of D299/D304
effect,, The authors conclude that the postradiation administration of pro-
tective agents to homiothermal mammals cannot weaken radiation lesions of
the body. There are 1 table and 5 references: 2 Soviet-bloc and 3 non-Sov-
iet-bloc. The reference to the English-language publication reads as foll-
owsz D.E. Smith, Radiation Res., 12, 79, 196o.
ASSOCIATIONt Institut morfologii zhivotnykh im,, A,N,, Severtsova, AN
SSSR (Institute of Animal Morphology im. A.,N. Severtsov,
AS USSR), Moscow
SUBMITTED: July 26, 1961
Card 3/3

32750
3/205/61/001/006/010/022
D268/D305
AUTHORS: Alekseyevap Yeja~j Korogodin, V.I.,
Nekrasova, I.V., and Tambiyev, A.Kh.
TITLE: The effect of cell suspension density on radiosensi-
tivity of yeasts
PERIODICAL: Radiobiologiya, v. 1, no. 6, 19619 878 - 886
TEXT: The correlation between concentration of suspensions and
radiosensitivity was studied in 5 yeast strains: the haploid Zygo-
saccharomyces bailii, diploid Saccharomyces vini Megri 139-B, and
3 strains of S. cereViBiaet haploid 127-12 d, diploid WY-110, and
tetraploid 16 x 32. Strains were cultured on wort agar at 28 - 300C
and irradiated after 2 - 3 days development. Either aliquots obtai-
ned by scraping hard medium or by centrifuging dense solutions, or
suspensions with a concentration of 109 - 1o4 cells/ml. were irra.-
diated. A PYn-200 apparatus (RUP-200 industrial X-ray unit 200)
with a dose rate of 5 p 400 r/min. was the X-ray source, and a ]-,,,T-.
Co-400 apparatus (GUT-00-400, therapeutic gamma unit Co 400) ihe
Card 1/5

32750
S120 61/001/006/010/022
The effect of cell suspension D268YD305
gamma-ray sources Strains were also irradiated in 5 - 10 % egg pro-
tein solution. Cell viability was determined 0by counting macro- and
micro-coloniesp incubated on wort agar at 30 0, according to me-
thods previously described by Korogodin (Ref. 8-9: Biofizikag 2,
1789 1957; 3, 206, 1958). Oxygen content in aqueous suspensions at
different concentrations was determined polarographically by a me-
thod described by Konstantinova and Grayevskiy (Ref. 10: Dokl. A.N
SSSR, 132, 14279 1960). Aqueous suspensions of the 3 S. cerevisiae
strains exposed to X-rays showed a fall in dose effectiveness as
the cell suspension concentration increased. The oxygen content was
determined polarographically in suspensions at different concentra-
tions. Results showed a clearifall in oxygen tension as the suspen-
sion concentration increased. Respiration intensity was determined
in Z. Bailii and Megri 139-B and showed that the Q02 for the former
was 840 � 156, and for the latter 3,100 � 320 Pl./hour for 1010
cells. It was much lower in haploid than in diploid cells, Accord-
ingly the concentration effect would be weaker in Z~ bailii than
in Megri 139-B. If the effect were due io oxygen deficiency, sus-
pension concentration would affect radiosensitivity rather less
nPrd 2/9

32750
S/205/61/001/006/010/022
The effect of cell suspension D268/11305
with irradiation in oxygen-free conditions than in aerated waterp
especially in a strain with low respiration intensity, This would
be in line with the views of Gunter and Kohn (Ref. 3: J.Decteriol.,
72, 422, 1956). 106 cells/ml. suspensions and aliquots from both
strains were exposed to gamma-irradiation in the atmosphere and in
a vacuum, and viability determined according to micro-colonies. Re-
sults completely confirmed the proposition. The dose effectiveness
reduction coefficient for the haploid strain irradiated in air was
0.819 and for the diploid 0.47. In conditions of anoxiao no concen-
tration effect was observed for the formerg while in the latter the
dose effectiveness reduction coefficient was O,~81. Oxygen content
in suspensions in a vacuum was 3 - 5 % copared with that in dilute
suspensions in the air. Irradiation of 10 cells/ml. suspensions
of haploids and diploids in 5 and 10 % egg protein solutions with
gamma-rays showed no protective reactions by the proteins. Accord-
ing to Gunter and Kohn yeast cells are also only very mildly sen-
sitive to H202. Tests were made with 4 strains. Results showed that
though they differed in their sensitivityq haploids being most sen-
sitive, H202 only affected viability noticeably at concentrations
Card 3/5

32750
S/205/61/001/006/010/022
The effect of cell suspension ... D268/D305
of 13.4 and 28.8 pg./ml. Experiments were also made to determine
the effect of suspension concentration at the time of irradiation
on post-radiation recovery with Megri 139-B, whose post-radiation
recovery has been already described by Korogodin (Ref. 7: Biofizi.-
kag 3, 7039 1958). Exposure was to gamma-irradiation. Part of the
suspension was sown on nutrient medium immediately after irradia-
tion and part at 24 - 48 hours. Viability was determined by macro-
colonies. In both cases change in dose effectiveness was largely
dependent on suspension concentration at irradiation. The extent of
post-radiation recovery of yeast cells was virtually indepen,1ent of
their concentration at irradiation, the dose effectiveness reduc-
tion coefficient fluctuating within 0.41 :t'O~,03- It is concluded
that at concentration effect was produced when yeast cells were ir-
radiated with X- and gamma-rays in normal air and in one case with
oxygen deficiency. Radiosensitivity was ind.ependent of suspension
density up to a concentration of 106 cells/ml., but increased pro-
portionally to the concentration logarithm with a further increase
in density. The concentration effect was more pronounced in the
strain with greater respiration intensity, The very poor sensitivi-
ty of yeast cells to H 0 was demonstrated, as well as the reduct.-
n~ 'A AIC; 2 2

32750
S/205/61/001/006/010/022
The effect of cell suspension ... D268/D305
ion in oxygen tension with increase in suapension concentration.
There are 5 figures, 3 tables and 14 references: 8 Soviet-bloc and
6 non-Soviet-bloco The 4 most recent references to the English-lan-
guage publications read as follows: S. Gunter and H,, Kohn, J~ Bac.-
teriol., 729 4229 1956; T. Alper, Radiation Res., 5, 573v 1956i T,
Alper and N.E. Gillies, Radiation Res., 9, 86, 1958; N.E. Gillies
and T. Alper, Nature, 1839 237, 1959.
ASSOCIATION: Biologo-pochvennyy fakulltet MGUq Institut morfologi'
zhivotnykh im. A.N. Severtsova AN SSSR, Moskva (Biolo-
gical-Soils Faculty, Moscow State University, Instit'-
u-
te for Animal Morphology im. A,N. Severtsov~ AS USSRj
Moscow)
SUBMITTED: July 26, 1961
Card 5/5

GRAYEVSKIY9 E.Ya.
International symposium on primary and initial processes occurrirg
in living cells during the action of ionizing radiation. 'gap.
sovr. biol. 51 no. 2:257-260 Mr-AP 161. (MIRA 14:4)
(RADIOBIOLOGY-COIIGRESSES)

20366
S/020/61-/136/005/032/032
211 $00 B103/B208
AUTHORS: Grayevskiy, E. Ya. and Konstantinova, M. M.
TITLE: Mechanism of antiradJation effect of dithiols
PERIODICAL; Doklady Akademii nauk SSSR~ v. 136, no. 5, 1961, 1219-1222
TEXT: The authors studied the antiradiation effect of dimercapto
compounds: 1) of unithiol,. and 2) of dimercapto propionic acid (DMPA)
on white mice. They compared the efficienoy of these dithiols with the
hypoxia caused by the latter (due to otygen tension). There are only
few contradlotory Onttt avt0laWo in hin prot)lom, Tho mioe were
totally irradiated withy-raye of CA with a dose of 357 r/min, and
with an absolutely lethal dose of 900 r* once for 2 min 28 see. The
mentioned protective substances were injected subcutaneously in 0.5 ml
distilled water in the following quantities-. unithiol: 20, 14, and 91
DMPA 1.0 and 0,75 mg per animal. Unithiol was applied at 11 intervals
between 10 and !80 min, DMPA at 6 intervals between 15 and 180 min prior
to irradiation. The criterion of the efficiency was the percentage rate
of the surviving animals4 and the lifetime of the killed animals up to

20366
S/020/61/136/005/032/032
Mechanism of antiradiation effect B103/B208
the 30th day from irradiation~ The change of oxygen content iii liver
and spleen was determined polarographically within 3 hr after injection
in vivo and in vitro by means of a platinum electrode of the "open
type". (Methods have been previously described in Ref. 2). The curve
illustrating the percentage of the animals with a reduced oxygen tension
(by more than 50%) (Fig. lo curve 4) served as an additional criterion.
The authors found this to be the minimum of hypoxia which effects a
protection from radiation. They draw the following conclusions from their
experiments! The two substanoes tested effect a distinct protection
which remains active for a long time after injection. It is striking
that lower dithiol doses (within certain limits) exert a more powerful
protection than higher doses. This may be explained by a higher toxidity
of maximum doses'of dithiols for irradiated animale. Such doses are
better tolerated by non-irradiated mice~ The two tested protective
agents reduce considerably and for a long time oxygen tension in the
tissue. This effect coincides with the time of the most
pronounced protective effect in unithiol doses of 14 and 9 mg, and in
DMPA doses of 0.75 mg. The authors conGlude from the data obtained
- -1,

20366
S/020/61/136/005/032/032
Mechanism of antiradiation effect,... B103/B208
that the protective effect of dithiols is probably due to hypoxia
caus
ed by these dithiols in organs sensitive to radiation, in contrast
with the effect of the best known sulfur-containing compounda (0-mercapto
ethyl amine, or 2-aminoethyl isothiuronium-B-HBr, etc.). The formation
raechanism of this hypoxia could not yet be'explained. There are
2 figures and 8 references: 4 Soviot-bloo and I non-Soviet-bloc.
ASSOCIATION: Institut morfologii zhivotnykh im. A. N. Severtoova
Akademii nauk SSSR (Institute of Animal Morphology imeni*
A. N. Severtsov, Academy of Sciences, USSR)
PRESENTED: August 22, 1960, by A. I. Oparin, Academician
SUBMITTED: August 18, 1960
Card 3/6

GMkYEVSKIY, F.Ya.; KQCNSTANTINOVA, M.M.
Independence of the radiation protective action of aminoethyl-
isothiuronium - Br -"HBr from the "oxygen effect." Dokl. AN SSSR
14r no.3:705-708 S t6l. (MIRA 14-9)
1. Institut morfologii zhivotnykh im. A.N.Severtsova Ail SSSR.
Predstavleno akademikom N-M-Sisakyanom.
(Pseudourea) (Radiation protection)

P7 /z-
I-Ilextinng on Radiation Protection In Mammal%
E. Ya Cr4ii;y. N. F. Darakfiss, At. M.-Conitand- ad 1. 1). S.1.01.

R4,11.1han Prolet lum and physiolosi" I c(Ttet can be divided by their mechanism of
t- varying to their stnK
protective mWon into W, Stoupi. 00C group acts by c.tuiing tissue hypswa, white the pfiltestive ufi,,n or the
second group appears not to be related to the oxyVn citect.
Proiccloriol'the second Froup shm, a clear moff,holosical protection ofaninufliesposed loradiallon.docteasing
the damage to the Intestine and hactnopoictic tissues. Under the sclion of an example of this lirpurs, lunino-
c1hyllsouroniurn-Dr-lifIr (A fl, repair processes are o=lcrntcd. and N~cr chromosomal aberrations am seen I

t and the ability orceii, to undergo division is restored, although there is no diminution in the initial iturnbcror"ns k
of the intestinal crypts disrupted as a result of I"adial ion.
114cmutinlictic tissue, irradiated In thepresenceafAr.T. shows a greater number orintact imils and regeneration
it greatly accelerated.
'I he Intensification of repair rrocess" observed In radiownsitivs: tissues actemi to be determined by a smaller
initial damage of their component cellular elements.
1~"-& f A-d A-,t-p at Sje- sAe USSR. Alanve

rOPOrt FMIOnted at tZw ad Intl. CwSmas gr V5dj&tjOft peggarch
,
x4rrognte/r-kahim. ot. Brit. ~-n Aug X962

9
S120y'0'2100210011.007/.U"! G
C2 ~, 10 10 D268 D302
AUTHOiS: Gr-"evskiv. -*-P-.Ya., Nekrasova, T.V., and Shullnina, !.I
TITLE: A study of the radioprotective action or. protozoa ol'
some Drotective substances
PE-116IODICA-L: _Radiobiologiya, v. 2, no. 1, 10,62, 148 - 155
I OU ~" 0 n1 a:".
Tl'-',(T; The radio prote c -t--ive effect of AET (aninoethyl-iso,~1"
Br. HBr) at 0.017 and 0.0033, cysteinami-ne at 0.005 - 0.02, cysti-
na-mine at 0.0017, 0.00711, and 0.0166, and heroin at 0.0017 - 0,0066
mg/ral. vias studied in "Parai-,ieciur.,i caudatum" cultured individually
in Lozina-Lozinskiy medium at 2100. AET aas dissolved in t',,Ace dis-
cilled water, made alkaline with NaOH to pH 6-9 - 7.2, and the
other protectors in twice distilled nrater alone. '.-fter 15 min.
t'---e respective medium ilifusoria were irr,~diat-ed aith. Y.-ra,rs at a
dose of' 100 kr. at 1 - 30C with 100 "Para-mecia" /0.1 ml. medium 4n
glass vessels within a plexiglass container in w1aich vacuum cond.:
tions could be produced. ART clearly increased survival of infuso-
ria both with DH 5 and neutr_-1 nedium and there was some Drotection
Card 1/3

S/20 o2/002/001/007/010
A study of the radioprotective ... D268YD302
.L - -- -
takinc, t-11e fission rate of the infuso-ic--i as a crite-2ium. uystcixna-
mine also incroased 3k1rViv,-,t1, but railed to tl-,e effact- of
had a
radiation on tile fiSsiOll lote. At 0.0166 i-C,/ml. 1,W.*,,0VV,*, i.
somewhat adverse effect in uoth respects, even with irradia 4-ion in
vacuo. Cysteinamine and heroin -a-ve no protection. Nore of the su')-
stances increased -orotection. wit-h irradiation in vacuo. Injury to
infusoria under ir-~'usoria under i2~radiation to a lar6e extent was
due to the Droducls of the radiolysis o-f vrater, and by the :!ecl-;;.ne
in radios ensitivity accompanying increase in their co-ncentrFtion in
the acueous medium. The effect of the orotectors vicas studied on
hydroven oeroxide Y-iold under irradiation ar-,.' its con7ent in irra-
d-L_--Ued medium to which the protectors viere added inunediately after
irradiation, with negative results. -AM ad4ed to tlie medium
ied normally and in vacuo immediately foiio,.71'1'1~1 i"1'rC-1q`-;&-tion
reduced toxicity to irradiated and non-irra'di-a-ted infusoria. It IS,
z.here2ore, concluded that the Drotective effect of ;'.ET was at least-
pa.-tially due to iTs action on The orCanism, z~s a res-ult of which
susceptibility to nydroger. peroxide and possibly to o-%,*,-.e-. products
of the radioiysis of water ,-ias reduced. A7-T- irradiated at the con-
Card 2/3

A study of the radioprotective ... D268XD302
centrations studied had some zoxicity for the infusori_c, and ma,371-,ed-
ly increased the injurious effect of irradia-Ued aqueouv~ solutions.
---he comoaratively wewlk protecTive effect of oil infusoria irra-
diatea in aerated soiut-ionq and the absence ol" any supplernent-ary
-orotective ei*fecu in iacuo are thou-ht to be due to -uoxicity acoui-
red under irradiation. There are 9 fig-ures and 5 references: 4 60-
viet-bloc and 1 non-Soviet-bluc. -.ehe reference to z'"e
.-uage z)ublical' iost reacis as follows: H_w- XU-bali, '~ml. 'N.Y., Acad.
Sci .,- ~9, 638, 1955.
ASSOCIATION: Institut norfologii zhivotnykh in. JIL.N. Severtsova AN
SSSR T.,:oscovt (institute for -Animal l'or-phology im. A.111.
Severtsova, AS USSR, Mlo.scow)
SUBM'I'L"TED: July 26, 1961
Card 3/3

GRAYEVSKIY, E.Ya.; SHAPIRO, I.M.
,IU. Urbakh's 'hook *Mathematical statistics for
and physicianO. Radiobiologiia 3 no.4:628-/629
(MIRA 17:2)

GRAYEVSKIY F.Ya.; KONSTANTINOVA M.M, NFUASOVA, ..V,,, TPRASENKO,
-- 10- - - y
Mechanism of the radioprotective action of' eystamine (2-aminoethyl-
disulfide), Radiobiologiia 3 no. 6:8q1-897 163. (MIRA 17i7)
1. Institut morfologli zhJ-votnykll Imerl. A~N-',everl--;owt AN
SS,SR,l Moskva,

I
Theoretical aspects of the chemical protection of mammals
against idbizing -adiation. Analele biol 17 no. 4:35-57
J1-Ag 163.

GRAYEVSKIT..,_P-,,,__)[4.
Theoretical aspects of chemical protection of mammals from
ionizing radiations. Zhur. ob. biol.24 no.1:3-22 Ja-F'63
(MIRA 16:11)

GRAYE;VSKIY., E.y&; BARAKIIIA) N,F.; KONSTAM'INOVA, M.M.; SMIMOVA, I.B.
I ~.... .. 1------.-
Studies on radiation protection in mamnals. Zhur. ob. biol,
24 no.3:182-193 MY-Je'63. 01M 16:8)
1. A.H.Severtzov Institute of Animal Morphology.. Academy of
Sciences of the U.S.S.R., Moscow.
(RADIATI03-FROrECTIVE AGENTS)

GRJkYEVSKIYJ, E.Ya.; KOROGODIN, V.I.; KUZIN, A.M., ; MOSKALD!,
m- -Tt-.T-.-WUWOVj K.V.; STRELITSOVA', V.11.j SHAPIRO, N.I.,
doktor biol. nauk; SHIKHODYROVp V.V.; EYDU3, L.Kh.;
ALEKSAKRIN, R.M., red.
[Principles of radiobiology] Osnovy radiatsionnoi bio-
logil. Moskva, Naiika, 1964. 1,02 p. (NIIRA 18:1)
1. Akaderdya nauk SSSR. Institut biologicheskoy 'Lizlki.
2. Chlen-korrespondent AN SSSP (for Kuzin).

ACCESSION NR: AP4027966 S/0205/64/004/002/0197/0202
AUTHOR: Grayovskiyp E. Ya.; Zherobehonko, P. G.; Konstantinova,
M. ~1.; -'R,--;'-'-'3hevchenko, A. N.
TITLE: Relation of ridioprotective activity of indolylalkylamines to'
tissue hypoxia and the role of vasoular changes in its development
SOURCE: Radiobiologiya, v. 4, no. 2, 1964o-197-202
TOPIC TAGS: radioprotootive action mechanism, indolylalkylamine
radioprotective action, tissue hypoxia, vessel spasm, tryptomine
der*vative, radioprotective preparation, 4-,5-chlortryptamine,
4-,5-metoxytryptamine, serotonino, alpha-mothyltryptamine, LSD,
eystamine, oxygen Intensity, eystamine radioprotective action
ABSTRACT: Literature studies have established that indolylalkylamina
radioproteetive action is related to tissue hypoxia. This work
investigates 4 whether
V-he mechanism of this action by determining 1),
the position of a substitute in a tryptamine molecule affects its
capacity to p;-oduce tissue hypoxia, 2) how '' the introduction of
alph a-mothyltrypt amino and LSD affectathe h7poxio'and vasoconstrictive
Cardl/3

AGGESSION NR: AP4027966
Taction of the preparations, and 3) how the combined use of
.5-metox~trypt amino and cystamine affectsoxygen level and vessel
reaction in tissues. The following preparations were administered
intraperitonoally to experimental white mice: 4-chlortryptamine
(60 mid1crO. 5-chlortryptamine (60 mg/k,9)t 4;metoxytryptamine
(60 rzAf,0,5-n, ctok-jtxwrtn~dne (60-mZ/IZ)- - and' orotonine (50 mg/k
V g) i h~
after administering alpha-mothyltrwtamines eystamine (1504/kg)
combined with metoxytryptamine (50 ~ipjkg),, and LSD (10 m~/kg)
combined'with serotonine. Oxygen intensity in the liver and spleen
of the aninals i= measured by a polarographic method. Vessel tone was
determined by the accumulation of neutral red in the organs 30 min
after being introduced (65 mg/kg in a0.5 ml physiological solution).
Findings show that tryptamine derivatives with substitutes in the
U-h position (5-meton-, 5
fif4 -ohlortryptamine) are highly effective
radioprotoctors because of their capacity to produce hypoxia in
radiosensi-lu-ive organs by vessel spasms. Tryptamine derivatives with
substitutes in 10-ho fourth position (4-chlor-, 4-metoxytryptamine) do
not produce hypoxia or vessel spasms and are ineffective radioprotee-:
t,ors. Alphla-methyltryptamine and LSD remove the radioprotoctive
effect of indolylalkylaminos by preventing the development of vessel
spasm and subsequent tissue hypoxia. Cystamine enhances the
Co,d 2/3