TIVOIC,71Thc UST - At Vt-ta ca' Orijaal, Per-VAIL -- - V cll., rater...4ixl � .AllotT..--eit A...,:sAref (41 knt17,-..1.. iitz".7 on_ Cov.trel , 2,4 ---1 i E r---- , ao iltoi .4., - I ilfic-ice,-crip �17-1-t Tit-a r:xtr-.5.:1c' A A-11,7;7J3n:: Asys:nt t".f.` Coverni. l'a liv:eli'er Coll *tiro r-,,, ,.......th. - ..{4. �.4,0=7,11,e-.7--,., , al-.07.�...-V-Ti............ 9s, 'ej '7 ..%. .1e, 7 _ _.4..... 1 - Ace:tint . .v.".1v1ce. �71-- Project MIX De.te initiated Subproject 3.05 20 January 2.960 Date Zxpiress 30 Jure 1962 Ftntda-current year: $4,000.00 Purpose: This project supports research on determinants of virulence in pithegenic bacteria. It peovidee consultation services on problems rel d. ate tomedultenance virulence. In Vivo ard VI. be deve a 11 1 1 li II 1 I 1 1 [1 11 Stat.i.ua: A coati:met% research. This 10 al I long reseirch but Information his been detveloped. to I meet id. hoc requirements !derived fro a other but 1 related. ;orograms of TS0 interest. I I 1 1 characterization data eill� WeT eneloSeas 1961 through_ - - r e � t e e :apo Ogize for , not sending the e en ia -:;iithproper iMe_after_JUne3 ecOrAs�in through � ember therefore, statement _ for the =final sIx �month pe,riOCI gnow due...:The accounting_ records: shori:t6me.' ,un11,,qui4Ated :coskitaientt at th rend-lot the ,grant period :end �ve would icrefer-ito':WithhOld_the7f inalEi tat-merit until all payments haire-begni-mgde -to,,thatTwe may report : an ' actual cashJ balance -anfl*ake -a ra.rtiiidZof�the_ekact v amount. If this :is . not acceptable :� for -yaT:r14666;':, p/ease _advise us and we will prepare -4 ttatementibaSed _on xpe nd iture s_ and outstandi commitments - - flirec o o n acts '-. - Fiscal Administration Recei-sts: $ Cash Balance, July 1-, 1 170-22 Cash Received, !ovrib,:.r 10, 1961 1,000.00 Ch Received., December 1-, 1961 - 1,000.00 Cash Received, February- 21962 _1,000.00 Cash Received, April 20_,_1962 1,000.00 Total Cash Available Disbursements: General Supplies and_Services 702.07 Travel 1a.62 --Sbecial Services e.nd HonorariA 250.00_ _ _ _ Eauipnent Subtotal Indirect Costs $ 4,170.22 Total Disbursements Cash Balance-June 301962 23..68 Commitments: Cori tted as of June 3Q,11962 = Balance Uncomvetted end Unexpended February 4, 1963 tified Co et:_ _ iscal Administration 1,350.37 $ 2,819.8, 554-.80 -- $ 2 265.05 Date: s Mai a mut* taw t-ose grant mita all tamat ell , rt an Wag Cbgh 3. If this is. tins asA vs ena outs 11.�.celrat as Balance, uiy1,15i -- - Ctsh Received, tbvenber 10, 1..)1 Cesh Received, Decerter 11 163 Cash Received; PebruaryP, 1962 Cash Received, 4,AI 23,0 1962 Total Cash Mal-able Disburcenente; 170.22 - 1,000.00_ 1,000100___ 1,000.00 $ 4,170.22 Ocreral eviliceiteld-Servicee Travel 702.07 1A.62. -- Spootal Services:awl Reekraril= 00.00 Ev.ipzent � 4� Slibtetal: FM-treat COate Total Dictadvdwate Cash Baleace, Jule 501 1962 $1 ,32869 21;68 .."..ftebagsesr.s.r.agalftellet W5rdizontet (trial tie& 0 _ftle 30; 1962 7kurnce ted orkl_lb----aveeded Ce7rtified-Cormet; I have-examined an expenditures. .1�min $112,819.85 rsaa.vsmns~rars. ov the uohlittaiJ Chief TSD/Biological ranch February hp 1963 The did not send a report-last_JUlY to the They thought that sincecireigot a six months ex ens on a a report would be due after the books were closed is of January 1, 1963. At all events, they are preparing now a summary accountingifor_the year July 1, 1961 to June 300-1962, They will send me cop1ea_of:this within the next several day - M t on to you, Also, copies will-be sent to the In addition, they will prepare an account,ng-summa To the six rnnths-from_Jtly-1, 1962 to December 31) 1962. I will send you a copy of this six :months accounting when itit-avaiIablesi With best regarft, We Propose to isolate from _various animals (including man) by-nssal-swab--- - - organisms that are olaiSified by -usual bacteriological technigtietraseu -- aureue. Strains of:S.--_a4reus---are important as causative agents_of_infection_in man ands also, for prmduclion-_of_ a _toxin _that _eauses food _roisoning_ in man._ -- Strains of S. aureus�also-cause infeCtion in other animals.--Wo-ProPose to study in vitro anUIrV1 ehtliteriStACS _ of 3. atlrella strains from-variove -animal ________ _ Macias to dere-rm-i-niif:hostadaThtation_occra an 10c-otti-as does occur for exaa(03e, in tl-,e=salmonellae. characterization williu.clude test for various hemolysins, type of coagurire7_ linase_activity, Vt ase activity, - fibrinolysiss rhage-Pattern_entl-antibiotio_sexisitivity. In_vivocharacterization _ will include test for_dermoncorotim_toxins- enteeotoxins rnre.ctivifor_mice. 16 RESEARCH PLAN, A. Specific Aims To oharactenieTbyboWiti vitro and in vivo Studies StaK lococCus_____ aureus of various an i7 m771 to detrie-- tho 7317Frition of thate_microorganisma-octurs. For examPle, are S. aureus: isolated from human beings-different-from 8, aureus isolated:?roirrat B. Method of Procedure - Isolation fzva nasallevab_s (and infections where boils in men, arthritis inichickensi mastitis in cattle, bladder_infec-__ tions in mink) of S6-_aureus_uslng mannitol silt agar, "life� 110 agar, and tellurite glyerne_agar.--After_establishment of Pure oultures_deter- mine kind of hemolysinsi-Phage typo.4 type of coagulase, fibrinolysis, DM ass, and:antibietio sensitivity. In vivoTcharacterisa- tion will include test for dermonecrotic toxin, enteraTUN and infectivity for mice. Along tora aPProach-uould include serological studies of -Possible differences betweenistrains-using-the OuchterlonyjechniqUes-_ 0. Significance ot_This Research The biology of_the staPhylococci needs investigation to determine if there is adaPtation_to_different animal species. Such terms-asIniumann - staphylococci, and_!bovqnsu_staphyloeocci-are vsed without-a real-basis for their use aoccePtto mean the sourer of-the-Pattie-ea!, culture. D. Facilities Avail-able Laboratories and:_amimal rooms-are available. No unusual equipment is required and the usual-bacteriblogidal-equiPment-is - e Purpose o s a ratory daniMills by- trapping.Through theicoonerattonf Publi� obtain nasal swabs from the animals.- This then-- - - a s urce o _animals that have not teen in contact ulth-man. 2. PREVIOUS WIRK DONE_ON_THIS-PROJEOT! We have used most ofA-Wtechniques UAW in Paragraph Bi(Research Plan) in studies on the Pathogenesis of staphylococci. We have also -studied the in vitro characteristicaof stafhylococci causing acute bovine mastitis and _ ZEionitAcyine mastitis, We started to isolate- staPkyloc000i from nasal swabs of various animals. During the past year we have examined nasal swabs from deer, foxes, ekurasuand-CupessuMs 83 well as from laboratory:animais has rabbits_ and mice. We are_ourrently doing-laberatory in vitro tests on the staPhylotoccal cultures that have been isolated. 3. PERSONAL PUBLICATIONS!. 3. PERSONAL PO OA 0 SI(CentinUed) Is. BESULT$ OBTAINED BY:OTliERS:_ Many workers have characterized staPhylococci isolated *era from human beings (FaeV8i-D.-"StEitlatiz4p15 asult_and ItalRelation-to-- Disease" 1959, E. alvius Living-stone Ltd., rolaurgh and London), and from__ milk from dairy cattloL(Reid W. and Wilson-j-J.-B., Am. J. Vet'. Res.- 201_825_ (1959). There has also_been'extensive work_on_characterizatibn of_XPhylom-- cocci from infectionivin human beings, but limited work has beenidone --- characterization of staphylocoeci frOMTinfeetions in other animalaje4g. Smith, H. If., J. Path.-_andiBadteriol._41.1_73 (1954), Rountree_Wof Path. and Bacteriol. 72: 319 (1956) has done-Thage tyotng of etaphylocoeci7from nasal swabi from various_laboratory-and domestic animals. ThUsTit would seem apnropriate in a laboratory_where extensive work on staphylococci Per as is being done that a detailed inveatigation be carried_out_of_theLchatiaeristics isolated from varioua-animal-sDeeies. Research Assistant - Graduate Student Plus-sorac Student Labor 02140Q Culture 1iediaz$308 Glassier. - - $300 To attend sl To collect satarles nonoraria for visitors___$ _ 608 200 492 $11,009 300 WOREIGPROJECEISUMMARY _ Date: March 31 1960 Tide of Project: Name of Principal Investigator Institution to Which Principal investigator is Attached: SUMMARY OF PROPOSED PROJECT; (300 words �r1ess&eSe XII) Par. 1,, Statement of Policy) We propose to isolate fromNakotis animals :(inelUding ran) by nasal swab organisms that are-classified_by usual beeterioleTgifal�techniques as Stan1-4joceeous aureus.- &trails of_S. aurora are important as musatiVe agents of infocticirrirk�* slards Oleo, for pSAiro-t-ic7�i_of_a_toxirrl�that causes food_poisoning in pm. Strains of S. atireus_alto_cauin.fectiono ethanimale. We propose to ttdy in vitro ma iiiffro7olikracterfttics of S. enrols staini-froofyirionti animal species to deterif host_adaptation Ticotirin the staphylococci as does occur - for examleo in the salmonellae. In vitro characterization:willtWtest for various hettoVrinsi type of coa-strir47- lipase actiVityD11- ase activity, - fibrinolysiss, -OVA pattern -and "SiLt-ibiotid sensitivity. characterization _ _ will inoluie test for: delmonecrotio toktns enterotoxin, end infeetiVity for mice. APPLICATION FORA-GRANT Name of Principal Investigator: -- Amount Requested:_%___WL______ July 4_1960 Tide of Project: Name and Title of Principal_Invcstigator: Address of Principal InVCstigatoi:` - Name of Institution: Name, Title and Address of Financial Officer to Whom Check Should be Mailed As well as Exact Payee to Whom Check Should be No . for a period of_____teitelve_=T-=_months, from 30,0_1961 AGREEMENT: It is understood by the applicant and the institution to which he is attached that in signing this application (I) they agree that funds granted as a result of this recitiest are to be used only_for_the put � .ses set forth-in-this a ilication. and (2) they agree to abide by the policy of the as set forth in the "STATEMENT OF POLICY. Signature of Official Authort sign for nstitution Title of Authorize Of NOTE: Under column-entitled-"CIILER" indicate, if tiny, funds anticipated from other sources_to be rpplied_to_this project BUDGET Requested from Foundation PERSONNEL: (Professional and technical assistants, in eluding duties. Indicate names of profes- sional'personneLiLalceadelected) Research ASsistant_(Graduate-student to be selooted.)__ t2,11,8 er PERMANENT. EQUIPMENT: (Itemizi) CONSUMABLE -SUPPLIES: ( Itemize) Culture Moeda...0292 Animals -200 Animal Feed 100 TRAVEL: (State Pur To attend 0150 To study tech laboratory in TocoUeotee 150 860 OTHER EXPENSES: (Itemize) OVERHEAD: (S& "STATEMENT OF POLICY," Sec, VIII) Par. 7) T&A.1--H" Estimate of funds required to complete project if one year is not sufficient to bring to completion: thpoo Notes The prinoipal=investigator has 620)000 per yeer_from,thellffiand other sources to study virulence factors in staphylococcal infeetions. None-of this money is available for work outlined in the present proposal. _ _-c WESE-ARCHIPROPOSAL' Thie Department has had under investigition studies on virulent and non-virulent Staphylococci. It is expected that by investigation of biochemical properties and ntabOliITi functioning of the major genetic variar.ts, indicators would be identi- fied to-mark the virulent Sta-phylococci,T The project, therefore, hasgervetic, metabolic, and nutritional components. The varieties of to'llasrdted-by the various Staphylococci a,s well as the _ intensity will be determined as sub -are.-Is in this study. It is expected that the eventual ,goal of this investigation-will be bioehernical_tosts which_will identify the organisms of interest. Pay of personnel Travel Supplies PROPOSEDIBUDGE_T � ,200.00 200.00 600.00 Total $4,000;00 DRAFT 2Z December 1959 MEMORANDUM FOR: THE RECORD SUBJECT : MKULTR-A7Subproject 1os The .iurpose of subproject 105-is to support_investigations the determinants of virulence in Staphylococci.: Thi dlTis important fundamental investigation which can lead_to_an_increased under- standing of the process-of pathogenesis. .Z. principal-investigator. 3._the cost of this pro ram fizr-th first-year i 000. 00. will be the To this sum must be-addd MO. 00 Or a four-per cent service charge to the making the_total amount for one year $4,7160.00. Th. will function as cut out and Cover for this grant. The cover title o - � the project i 4. Tt:T>t-al cost of the project for a. period_of_one_year will not exceed $4,160.00. Charges should be made against Allotment No. 0525-1009:4902. 5._ :T h� has requested the Unive sity to submit e2 TSS Cherritcal-Division a sLrmn1ary accounting of monies received and to return any unexpeaded_grant funds at the end of the project._ year. 6. Title to any permanent equipment purchased by funds granted the University shall be retained by them in lieu_of higher overhead rates. 7. Ivas_mutually agreed th-at documentation and accounting for travel expenses which are reimbursable by the_UKiversity conform_with the accepted practices of that institution. 8.iTh requirement for a six months' informal_accounting on the part of_the_principal investigator is ytaive'd. ) 9. DT'. is cleared through SECIET1andisiawareofthe1IL true purpose-of_the program-. APPROVED-FOR OBLIGATION OF FUNDS1 rector Chief Date: TSShernical Division Attachment: Proposed budget and proposal Distribution: Original only MEMORANDUM TOR compritoLtra _ ATTENTION Vance Division SUBJECT 241tULTRA, Subproject 105 - �'Undo? the authority grouted erandQm dated IIIL1I III 13 AprIl 1)53 ham the DC1 to the DD/A, acd tho_eotocslon of this authority in subseveat mamoranda, Subproject 103 hae b approved and $4,160.0-0 a ail over-all MKULTRA, project Nadi has boon obliwitod to cover the subproject expenses. -T obligation of fund* should be charged to Allotment 05251005-4502. AP ROVEIXFOR_OBLIOATION O' FUNDS: 1101.44M*AradWirkinaNIVer.ordawne.lemPup...1.a...4, ReceaTcit Director . Distribution; Orig tt 2 � Addresses 1 - TSS/OC 1 � TSS/FASB - TSS/CD , TSS/CD (Z9Dec '59) INVOICE For Service **cat e.eass a ems e_ �-� 0-** CERTIFICATIONS Alt ek � an. ens. (1) It is hereby certified that this is Invoice No. 1 applying to Subproject 105 of MKULTRA, that performance Is satisfactory that services_arelniog accomplished in accordance with mutual agreemenks, that detailed agenda of the payments and receipts Li ou filo in TSD1CB, that this bill is just and correct and that payment thereof hail not yet been made. Date: CttlTafTSD/Chernical Branchr (2) It is hereby certified that this invoice appllei-to ZAKULTRA $obproject 105, which was duly approved, and that the project is being carried out In accordance with the memorandum dated 13 April 1953, from the D_CLtoAha DD/A, and the extension of this authority in subsequent memoranda, Date: Research Director 26 Ap:111960__ MEMORANDUM OR;_CRIEri FINANCE DIVISION VIA � TSD/Budget Officer SUBJE � : MKULTRA, Subproject LOS, Invoice II Allotment 0S25-1009-4902 , I. Invoice No. 1 covering the above subproject it a'it Payzatrit shOeld be made as follows: Cashier's check in thcarnount of payable t Ca hier chock in the amount of $160.00 d payable to 2. Please_forward_the _checke_to_Chief, TSD/Chimical Branch, through TSD/Beclget Officer, ao later than 9 May 1960: 3. This Is a flosl_involce._ However, slate It is anticipated that additional_funds_will be obligated for this project, the files should not be closed. Attachrneets: Invoice kCertification. Distribution: *Prig lc 2 - Addressee 1 � TSD/FASSTIII 2 -=TSD/CB TSD/C Chief - ?SD/Chemical Branch funds: We are-pl-e-a-s-ed to be able to transmit to you the following 1960. k G41068 drawn on for $4,000.00 dated May 21960. drawn on for $160. 00 elated May These funds represent a contribution for the use-of your directors in carrying out the very worthwhile research goals of your organization. - Yours_truly, IS April 1960 MEMOR D ) ORiCH1Et. FINANCE DIVISION VIA 3 TSD/Budget Officer SUBJECT I AIKULTIAA, Subproject 105, !evoke 01 Allotment 0525-1009A9OZ I. Invoice No. 1 covering tho above subproject le attache& Pa eat ehoald be toad, AS tOILOWIS Ca O. la the amou payable to 2.1 Please forward_the checks to Chief, TSD/Chemical Breach, throush TSD/Budget Officer, ao later that 9 May 1960. 3. Thit le a nest invotca. However, place It la aaticIpaied that adJltioall blade will be oblisat'il for this project, the files should sot be closed. Invoice k-CettlfIcatleas Distributioat ctrig It 2 -Addresses 7KTSD/FASS TSD/CB TSD/CB CUTITT kNZ.,1S Akt AVAI .9L irkiCAltOri_KERti:X.4 14. uppsE io ott

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MKULTRA SUBPROJECT 105 (23 DOCUMENTS WITH PUBDATES FROM 591222 TO 63020 4)