3ue Nor 1, 1962. $3,852.00 Due Feb 10 1961 3,852.00 Due May 1, 1961 3,853.00 Due Aug 1, 1961 3,853.00 TOTAL GRANT $15,410.00 Nov k1, 1959 Feb May 1, 1960 Aug 11 1960 Status: rival fractions in being stattlied an. the Riven corembosa . sepantionr constidsrable regress has been made on the separation of the active constituents- or Amanita tatureazia, and a start has been made on the separatist of Ritynchosta SUBMITTED BY VOUCHER NO. (Finance use Only) ACCOUNTING BY INDIVIDUAL MKULTRA- Subproject #22 FOR ADVANCE PERIOD OF ACCOUNTING NOTE: Follow Instruction. on Reverse FROM 1 November 1962 TO 15 July 1963 RECEIPTS DISBURSEMENTS I. CASH ON HAND BEGINNING OF PERIOD 7,705.00 MOW DATE OFSCISPTION AMOUNT it. $ .RECEIPT NUMBER DATE DESCRIPTION See attached certification --. Invo/c #11 TOTAL EAPENSES * 7.705.0111 sa. 4. REFUNDEO HEREWITH 1 loam 1 IGNECE MONEY 5., CASH ON HAND END OF PERIOD TOTAL TO ACCOUNT FOR 7.705,00 TOTAL ACCOUNTED FOR $7.705.00 I CERTIFY FUNDS ARE AVAILABLE APPROVED I certify that the attire's listed h d OBLIGATION REFERE7ICE NO. 639 CHARGE ALLOTMENT NO. DATE SIGNATURE OF APPROVING OFFICER SIDNEY GOTTLTER,ECLITOIthis on any attachaentm were incurred for official put' peso of a confidential nature. that trafamot or credit therefor hoe not boon' received, act that accounting le true and correct. DATE EMMATUREOFAOTHORIMOOFFIEEA CERTIFIED FOR PAYMENT OR CREDIT SIGNATURE OF PAYEE Sr DATE . SIGNATURE OF CERTIFYING OFFICER 2 6 JuN 1964 SPACE BELOW FOR EXCLIFIVE USE OF FINANCE DIVISION RED BY i' REVIEWED BY VOUCHER NO. 7.12 I OESCR/PTION � ALL OTHER ACCOUNTS 13.33 S4.39 40.42 43 45.45 All..? 54.67 55.6 t3S.TO � ELI NO. STATION CODE EXPENE F U PAY ar:116. 3 GENERAL ALLOT. OR COST ACCT. NO. out DATE AMOUNT DESCRIPTION ' ADVANCE ACCOUNTS 12.27 ..... ..... 5.0. 140. Poor. oo. CODE N D PER. LIO. AD!:VANCE CA YR LEDGER ACCT. NO AE.AT CK4 NO. �..... DEJECT ' suu . 'span ND. a 3 CODE �f�!?: IMF. NO. A ritt.mo. CLASS 0IMIT #11111I IIII 1 I / --, 1 I. 1 1 1 1 1 1111111 I I II I I I I Ill. ggggggI IIIIIIIIIIII I i I I I I--4�", I I 1I , I I I I I 4 A1111/1111111 1 1 I-1 1 1 ' A -,IIIIIIIII I I s I I I I- l / wi TOTALS -I-. 1 I I i 1 Fron:4.11.111101fPrincipal investigator Final fina .* renewal of reseazch,Kant , entitled Period of grant: Noyember 1,-1962 - July 15, 1963 For professional servios rendered !Sreach -------- ----- Travel expenses return by auto Total expenditures $7705. Total funds from grant 7705,, Balance -a- This is a correct accounting of funds received by me SUBMITTED BY VOUCHER NO. (Finance use only) b ACCOUNTING BY INDIVIDUAL HEUL2M__44.0 23 FOR ADVANCE PERIOD Or ACCOUNTIUG () NOTE: Follow Instructions on R FROM 11 Noveetber 1961 TO 3X October 19(2 RECEIPTS DISBURSEMENTS I. CASH ON HAND BEGINNING OF PERIOD :WAIN DATE DESCRIPTION AMOUNT 31 Oet � 6 DATE DESCRIPTION 19Ce See attadbed certification Tm 11 Nov UMIJOIDA Advance 1961 Invoice$11 00111,11 15:40.00 ill/ TOTAL EXPENSES 7 705.00 3a. 4. REFUNDED HEREWITH CAP CHECK i�" ORDEN 5. CASH ON HAND END OF PERIOD 7 5,42 TOTAL TO ACCOUNT FOR /15.4103.00 TOTAL ACCOUNTED FOR $ 35 10 00 I CERTIFY FUNDS ARE AVAILABLE APPROVED I certify that the expenditure. listed h 00000 and incurred for official ORLISATION REFERENCE NO. s39___amNmmia CHARGE ALLOTMENT NO. , DATE SIGNATURE OF APPROVING OFFICER , i AIMS GOTTLIEB, OC/TOO on any at tachstents ware pun poses of a confidential nature. that pay er credit therefor has not been received, and that this eveountint II true and DATE SIOWUREOFAUTHORIZINGOFFICER CERTIFI ED FOR PAYMENT OR CREDIT SIGNATURE OF PAyEE .'� DATE SIGNATURE OF CERTIFYING OFFICER SPACE BELOW FOR EXCLUSIVE USE OF FINANCE DIVISION :40ANED DY REVIEWED BY VOUCHER NO. 7.12 DESCRIPTIO . ALL OTHER AccOUNTs Is.ss 4.311 S?i:hrN 40.42 42 45.46 laW .. 64.67 $6.67 ALLOT. OR COST 66.70 DUE 71.60 AMOUNT E EXPEN r BAY REF. NIS. " GENERAL ACCT. NO. DATE , DESCRIPTION' ADVANCEACCOUT NS19. 2/ Tit. P.o. o . POOP. NO. CODE PER. LIO. 00000 co ACCT. MO. CA VR LEDGER ACCT. NO. 62.67 Cr. tIO� *LIEGE I PROJECTIVO. F.,. S CODE -evic 0000 6 ete.140, CLASS I I lllll I I � 1 III t 111111 1 i I t IIIIIIIIIII I I I � 1111111111 I I I i I I I 111111 1 1 1 1 I I i v 1 1 i 1 1 1 1 1 1 1 1 1 I t TOTALS I i OFFICE OR RESEARCH ADMINISTRATION Incorporated Dea Your heck for $2,575.00, representing the third quarterly payment for research work, was received today. Thank you for taking care of this matter for us, Very truly yours, 44111,11,1!!!!Ich Administrator V. V. ItIUSINHIS �MCI Report of Expenditures ' November 11, 1961 to October 31, 1962 Grantor: Grantee: Fund: Income: Expenditures: Salaries and Wages $14905.00: Travel:- 1,921.65 Equinment 2.,252.99 Supplies 1,206.34 Other Expenses: Telegraph service 9.27 Telephone 169.06 Miscellaneous . 87.06 H265.39 Overhead @ .1376 of direct costs 1,053.63. Balance . /7 /fir- $7,705.00 7,705.00 -o- I certify that the-above Statement of account is -correct'te the best of my know1ed ass Manager 1 lave examinei and approved -C.ao submitte.f. expenditures. ace: TSB/Biological Brant); MEMORANDUM FOR THE RECORD SUBJECT pit 6 August 1962 to Pik)/ : ,xtension'of Grant or Authorization was given this dite-W10110 extending�gainlier ant time until 1 August 1963. C ef TSD/Biologital Research � MEMORANDUM FOR THE RECORD SUBJECT 26 March 1962 : Support Of Research. o 1 This date discussed seo t physiolo ical with es igat on is_ pro' his laboratories in progressed to the point where i more detailed phases. tc;�40-2 in detail the 'status of our re- ti vie ri ciples of the mushroom the principal in- inding progress in -. The research has is impor ant to engage in 2. In discussing the projected researCh, said that he was unable to engage in certain critic nass due to lack of contact with certain types of apparatus. He was of the opinion that it was most important to bring into the research picture an outstanding research worker who was knowledgeable in the field of drugs natural products: 3. Following were held between the consensus t laboratories of 141111,was inc peeagreem p rm., him to devote full time to his researe - same time, obtain much needed information on researches first hand. discussions myself. It was ' the 4. pointed out, however, that he would be unable t5�ry out-such-a.prosram without additidnaiugort. He stated that he had been in correWaTeEde witn and had received several cordial invitations to work.ip in excess of 10411 pointed out that his expenses awing to the.-fact that hewould have his laboratories. won family to transport and take care of. He was advised that the grant could not undertake support to this extent, but that it would be possible to use about of the existing grant to re- imburse him for his professional serVices, transportation, and a nnrtirn.oLhis living expenses while in residence at He was informed that. he, .personally, his own arrangements about the additional stated that this was quite agree- e would take the necessary steps. tW have to m funds required. able to him and ape Arrangements were made --NQ bewflhbeldZrom iven in suPPort of' was concurred esearc � es in by Chief, TSD, BUSINESS OFFICE ReportcdExpenditures November 1,4!p)to November 10, 1.9610.) Grantors Grottoes Fwtds. 6 Incomes Expendituress Salaries & Wages (Incl. Fellowships) Travel Equipment Supplies Publication and preparation of thesis Other expensess e.P1 Telephone 130.25 Miscellaneous 9.85 Overhead 0 15% direct costs Total Expenditures. , Balance 17/7/te, gator -----t- $9,300.00 13,850.00 1 107 10 4 � 1,150.65 - 1,684.99 ' 148:10 146.11 , 1.213.05 9.300.00 -0- I certify that the above statement of account is � POITICt to the best of my - knowled Business Manager sotto he to transmit to on the following 'CONFIDENTIAL FUNDS POSTING VOUCHER VOUCHER NO. 7.32 'DATE 2.6 VOUCHER NO. 7-12 DESCRIPTION.ALL OTHER ACCOUNTS 13-33 34.39 STATION ' 40-42 A: 43'46 47�52 OBLIG. 53 se.57 58 47 ALLOT. OR COST 88-70 DUE 71 80 AMOUNT A; N. CODE EXPEND U PAY REF' NO. GENERAL ACCT. NO. DATE , DESCRIPTION. ADVANCE ACCOUNTS 13027 p.o. No. PROP. NO. CODE i N PER. LIO- telP1%. � CA YR LEDGER ACCT. NO. 82.87 01.00. 00JECT D[811 emir' ' � /M3711.0"JE � DIV. C; ;O. �P 1 � 0 g rnne -'''' INP � NO. ......... X RIP. NO� el.aSt � /21 4/0 ,t-,,,,,- .7t..."- 7.1:2�� -4' e .. i kW t � � . � � � _._. ' .. .. . /J- ��,fr 0 ioe . .. 1 /1 re , . ....... 1 , . ... . ..... a � � � .f r� 2 � ; 6 � � � � � � � � � . . . � � � � � � . . � � � � . . . i � � ; � �"� ;) ; .. . f I � � � . � . . � i � : : ....... a � , � � .. . � � � . � . , I � . � � .. : . ......... . . . � . io . . . . . � . I . � . . : . . 6 EXPLANATION OF ENTRY - TOTALS � � ,/,..0190 41 se: ilarif� DATE PREPARED OY DATE REVIEWED 10Y CERTIFIED FOR PAYMENT OR CREDIT _ DATE SIGNATURE OF CERTIFYING OFFICER _ PORN SO�110 606 on %view. tog 7.-nti*ti,747--7,t7tTvtt7 IN THE AMOUNT OF or IN THE ANiri-IE AMOUNT OF 5. � Attachment; laroica & Certification* Distributions Otis 2 - Addressee 1 - TSD/FASS 2 - TSD/RB 1.8 OCr I CERTIFY MAT FUNDS ARE AVAILABLE: :so 0sucwoot4 &Yr:me.? 613.2_ "AKA TO ALLC.",:ENT ' ACCOUNTING BY INDIVIDUAL FOR ADVANCE c NOTE: Folios Instructions on Reverse SUBMITTED BY MUM/ Sub 22 � 941.1CHER NO. (Finance nee only) PERIOD OF ACCOUNTING FROM 3.3. November 1.961 TO 33. October 3962 RECEIPTS DISBURSEMENTS I. CASH ON HAND BEGINNING OF PERIOD 9.32gar DATE DESCRIPTION AMOUNT Ia. 31 Oct � . E.RECEIPT NUMBER DATE DESCRIPTION .1,..I: See attached certification 11_31rri_n_Adrance � 1961 invoice # 11 TOTAL EXPENSES S se XR. 4. REFUNDED HEREWITH Chill CHICK MONEY 5. CASH ON HAND END OF PERIOD 711705003 TOTAL TO ACCOUNT FOR 535,111o.op TOTAL ACCOUNTED FOR X 15�410.00 I CERTIFY FUNDS ARE AVAILABLE APPROVED I Certify thet the d listed h d on any attachments were incurred for official put. poses of � confidential nature. ghat credit therefor has not been received, and that this accounting is true and correct. OBLIGATION REFERENCE NO. 30Manne_ CHARGE ALLOTMENT NO. DATE 10 APR 1963 SIGNATURE OF APPITNO OFFICER Cilginal son 0. h .1 a . a inakrigala Da 1..:,� DATE . 1 21 ihr SIGNAIVREOFAUTIKM12111GMFFICER CERTIFIED FOR PAYMENT OR CREDIT SIGNATURE OF PAYEE DATE SIGNATURE OF CERTIFYING OFFICER � SPACE BELOW FOR EXCLUSIVE USE OF FINANCE DIVISION PARED BY I REVIEWED BY VOUCHER NO. 7.12 DESCRIPTION - ALL OTHER ACCOUN $ 12.83 4. 9 STATION COD not. NO. 40.42 EKREN CODE 43 N p X 45.44 PAY PER. Lb. O CODE all ftEr'RO. � hi-0. iecin Ivry ; , - CA YR 54.157 GENERAL LEDGER ACCT. NO $6.8 ALLOT. OR COST ACCT. NO. 48.70 DATDUEE OBJECT CLASS 71.60 AMOUNT 1 OESCRIPTION- ADVANCE ACCOUNTS 12.27 J AIT. P.O. NO. , ; CRE�11 82.67 CE. NO. � � ..... � � 3. RE,....). 13433 DIV. PEOJEC no. tIIIIIIVIII I I I I I I I I I I I I I i I i II I I I I I I I 111111111111 I I i I I I I I I 1 1 1 1 1 I 111111 I 1 � I I 1 I I 111111111111 I 1 I I I �_ TOTALS For Services 4111111111111110 - � usivcacE CERTIFICATIONS tl) it is hereby certited that this is inveice 11 applying to Sub-project No, 22 a MKULTRA, that performance is satisfactory, that services are being accomplished in accordance with mutual agree- ments, that a detailed agenda of the payments and receipts is CM file in TSD/RB� that this bill is just and correct and that payment thereof has not yet been made, Chief, TSD/Research Branch CZ) It is hereby certified that this invoice applies to Subproject 22 of MKULTRA which was duly approved� and that the project is being carried out in accordancs with the memorandum of 13 April I953 from the DCI to the and the extension of this authority in subseqtent 121071074,244ac, Research Director Date 3 5 Remarks and References tWaL&tAs dant avy Obligations Incurred If sa�. n;�,..;1�04; � MEMORANDUM PORI COMPTROLLER � � 'ATTENTIONFinance Division SUBJECT ; Project 141CULTRA, Subproject 022 Additional Authorisation #9 . � . . Under the authority granted in the Memoranda -dated- 13 April 1933 from the.DCI to thee and the extension of this atithoritY in subsequent. memoranda. Subproject 22 has been approved, and $18,028.40 of the over-all project MICULTRA APPROVED FOR ' Chief, OBLIGATION OF FUNDS: - TSD/Research Branch Distribution: Orig & 2 Addressee 1 TSIVOC 7 TSD/FASS 2 - TSD/RB � .1 MUST NAT rINIn ARS 1"1.4,32: � pracrat--N trtt 4tt 63? sag". lq Nigias AUMttZINtur,;;ICal : Project MICULTBA, Subproject #22 1. The purpose of this Subproject is to provide funds to continue the researches of Prof.4111111111111Mon the isolation of compounds from natural products which have psychogenic properties. Prof. is concentrating his studies on isolation and identification of active materials in the fungus Amanita muscaria. Studies will be extended to active principles in Bhynchosia phaseoloides. 2. Progress in the studies of Amanita has been significant to date. It is believed that the chemical entity primarily responsible for the hallucinogenic effects of Axnanita muscaria has been isolated. Because of the extremely small amount in the fungus, and due to the lack of sufficient quantities of plant material the amount isolated has been insufficient for complete structure elucidation. Effort during the coming year will be directed to obtaining adequate supplies of fresh material and the carrying out of various extraction and concen- tration procedures. Chemical and biological techniques will be utilized to identify and characterize the active 'Principles which are .3. The will serve as cutout for transfer of fund i to the investigator. The estimated cost as Outlined in the budget proposal is $15,410.00. To this must be added the 4% service charge to the cutout, making the total cost for one (1) year� not in excess of $16,026.40. Charges should be made against allot- 4. A Memorandum of Agreement has been signed by the contractor whereby title to permanent equipment is retained by the SSW in lieu of higher overhead charges and arrangements are made for accoun 5. It has been mutually agreed that documentation and accounting for travel expenses under the grant which are reimbursable by the shall conform with the accepted practices of that institution. 6. Prof. holds a covert clearance under. Project MKULTRA and has agreed to comply with the terms of the Memorandum of RENEWAL OF A RESEARCH GRANT Investigation of the Physiologically Active Principles of Amanita Muscaria - and Other Plants of Potential Medicinal Value Grant date: November 1, 1961 - October 31, 1 62 Principal Investigator WPM Salaries and wages -- BUDGET PROPOSAL 1961-62 Post Dpctorate (12 mo.) Directbr's stipend (3 mo.) Student help $6,000 1,500 500 Travel -- � Meetins and travel in connection with work $ 600 _ - ep m r, 19 1,000 Sample collection and prepeiation 1,000 pounds of kmniul Muscaria at $2.00/1b., etc. $2,500 Analyses, literature and special chemicals e $ 500 Equipment Unimelt Melting Point Apparatus Rotary Molecular Still, Ozone Meter $ 800 Overhead 15% Gentlemen: We are pleased:to be ablet to transmit to you the following of - $8,634.57. pbcck awn on the of Alste4 -15 Dece or � 5.38. . . These fmads.represent a contribution for the use of your directors in carrying out the very .worthwhile research goals of your organization. DENORAN/I24 Mgr, MUMS DIVISION viA SSD err? - Sub ct pr;Se 22., Invoice #10 Allotment 11254009-1902 C- 1*: Distribution: CNALL'Z' ICI mire attolet (Dag & 2 - Addresses 41011 - TSD/FASS AmcLINTI sthiliCECEIV HECKair TEZ AMMLINT CHEM:a 7; RED. RECE;`,�.7.,..".1611A ; S y -v-r- CONFIDENTIAL FUNDS POSTING VOUCHER VOUCHER NO. 7-12 DATE 2.6 VOUCHER NO. 7-12 DESCHIPTION-ALL OTHER ACCOUNTS 13-33 34.SS STATION 40-42 4, 45-46 47-52 COLIC. 53 54.57 50-67 ALLOT. OR COST 60-70 DUE 71-60 AMOUNT 1111g. CODE EXPEND U PAY REF. NO. OENERAL ACCT. NO. DATE DESCRIPTION. ADVANCE ACCOUNTS 13-27 P.O. 440. PAO?. MO. CODE N D PER. LIO. teltn111 CA YR LEDGER ACCT. NO. 6247 CA.110, ',:;;;:;;: OSJ[CT DtelY CIMDIT 143 PR.O.ACN tv $ CODE .1. ' ;;,;.: ' NO. CLASS 5 Viiii ) :CIS it'i; tAitX i 0 � � irk f 1 f 9Y/ Ge ha Kt- i a ?a -3901-. 7/3-' 7 coe.nr . a . ' .� . a . Ii ... V/ � � h. if 1-7fl Gab a 140 2- - 7-1-- ta v 2-1-S" 46.4143 isil 10911 -An 5'6 3 4t-i, Vitt il LI lik . . : � ; .. ; ..," IA- G t duu , . . ' .. .. .. . I �� 1 . I � 4 . ... � � � i .I / : 1 I 1 I t . I 1 � � EXPLANATION OF ENTRY TOTALS 74fav 7 sif-rpe out j PREPAR der, i DATE REVIEWED EIY CERTIFIED FOR PAYMENT OR CRE01 DATE SIGNATURE OF CERTIFYING OFFICER .4, 606 ti For Services 111��SMOSIMPSd� CERTIFICATIONS (1) It is hereby certified that this is InvOice NO. 10 applying to Subproject 22 of NESULTRA, that performance is satisfactory, that services are being accomplished in accordance with mutual agreements, that a detailed. agenda of the payments and receipts is filed in ISD/ BB, that this bill is just and correct and thatisyment thereof has not yet been made. Chief, TED/Research Branch- Date: (2) It is hereby certified, that this invoice applies to Subproject 22 tmder MEM= which was duly approved and that the project is being carried out in accordance with the memorandum dated 13 April 2953 ft= the ria to the DD/A, and the extension of this authority in subsequent memoranda Research Director Date: � (3) It is hereby certified. that Under subproject 22 at the close of 7159 a balance of $665.4.3 representing unused funds remained in subproject 22. Additi.onal funds ( IT 1.125..1009-1902) bate been approved. for .continuing subproject 22. Therefore it is requested. that $665.43 of Intoice No. 10 be credited. saproject 22 as a return of unused. funds (n 9-25(2-75-902)s MR 2707. No. Cast Account 9.41/ ',lit 'etc /91.2 Object Class Date Remarks and References Obligations Incurred Obligations' Liquidated Unliquidated Balance 21 ro 6 7fita,' LZ:a. --.61:1 10 .g,,tivr, fin .e � ftr, � 2 &Ea: 1441 .17 4 ide,,, vd.0 Jig AY 10 Fr' 49.A.0. - 2 Wo a 7, ie.., eft. , s� � / .., _ _ _ t . � 15 November 1960 � MEMORANDUM FOR: COMPTROLLER ATTENTION : Finance Division SUBJECT : Project MKULTRA, Subproject 122 Additional Authorisation /8 Under the authority granted in the Memorandum dated 13 April 1953 from the DCI to the DD/A, and the extension of this authority in subsequent memoranda, Subproject 22 has been approved, and $9,672.00 of the over-aU project MKULTRA funds has been obligated to cover the subproject's expenses and should be charged to Allotment 11254009-1902. APPROVED FOR OBLIGATION OF FUNDS: TSD/Research Branch '4- Research Director Datei Distribution: Orig. & 2 a Addressee 1 TSD/OC /Kr TSD/FASS TSD/RB TSD/411111111.(15 Nov.160) it' � 15 November 1960 MEMORANDUM FOR: THE RECORD SUBJECT : Project MKULTRA. Subproject #22 1. The purpose of this Subproject is to provi4e funds to coritinur; the research of Prof. 2n the isolation of compounds having psychogenic and related prop- erties from natural products. Prof.,. is concentrating his studies on Riyea Corymbosae variety and Amanita Muscaria and has isolated some active materials which are of interest. 2. Difficulties in obtaining properly documented raw 1 materials in sufficient quantity for further research have been to a large extent overcome. Considerable renewed interest on the part of several drug companies of foreign-origin in , related fungi and plant materials believed to have central nervous system activity indicate that there may be chemical compounds in the natural products under study of considerable TSD interest. 3. Thane.. will serve as cutout for transfer of funds to the investigator. The estimated cost for the coming year of t To this must be added the 4% service charge to the cutout thus the total cost for one year will not exceed S9,1672. Charges should be made against allotment 1125-1009-1902. 4. A Memorandum of Agreement has been signed by the contractor whereby title to permanent equipment is retained by the University in lieu of higher overhead charges and arrange- ments are made for accounting for funds disbursed under the grant. 5. It has been mutually agreed that documentation and accounting for travel expenses under the grant which are reim- bursable by the University shall conform with the accepted practices ciS that institution. 'rotil. 1p. holds a covert clearance under Project MY micand has agreed to comply with the terms of the y anca of Agreement. /ROVE App FOR IGATI OBLN OF FUNDS: Rese? �ched: B ibution 7: ell Dire:act Date:11)1r let AttaAdSet Dist:: Original onl C ef 'TSD/Research Branch SUPPLIES and EQUIPMENT SPECIAL CHEMICALS SPECIAL ANALYSES LIBRARY SERVICE OVERHEAD (15% of Direct Costs) 2'00 - for renewal of support to investigate The Physiologically ,Active Principals of Amanita Musearia and Rives Corvthbosa' Principal Investigator . President PROGRESS REPORT for. Grant Fiscal Year:1959-6o During this year work has progressed to the point 'where crystal- line substances with physiological, action have been isolated from Rivea Corymboea and Amanita Muscaria. Rather than review all the work in detail two papers are attached which describe the _principle aspects of the investigation during the year. The first _of then is aper which" bis'been_submItted/for PUbileation- regarding Rivea Corymbosa. The second is an aaptiororr ardin Arcantta Wistaria which s e ented before the August 15-25, 1960.' t 14;1 vs' � � LT:* ;74. %..t.efLa7. .+Iccrik 00VOttfog;:30.!;!.,114!--7,7tr.4.:4ZA:e...trie,v..1kt-staithti�xcE,,pii. earalzrzturtupg:I.., � � � aitipta �-jusikaiur,i � 0.7 po Put; ...rte.:exert 3.TX, , ciiki st .- iststi: I iSlikA ct � Tatrc 14331!??M... i;IP74'4Y P 451:**q4;ra4% t . � ,Jskd t:414. e s.1 r Azol re tiLt.44. CIX?'512,11 ' 011.1d: stet) fo,c,61 octor ..,4 .. 1.)1,,, cv.,:ern , sift '..t.t41.!..nel ,,,....,4 , , � ' , , ,,, e . . 71. t 3 - � 1:i Faiksr car,,aatt"Ivr.,p;'7.;4. kim3 ,moLoe,1 " . , g -`b d � Research Plans for 1960-61. . As indicated in the accompanying progress reports and papers related to the research supported by the grant, several crystalline substance have been isolated from Amanita Mnscaria and Rives Corymbosa. Future work will be organized to accomplish the following objectives: 1. Ascertain, by means of biological testing, which, if any, of the crystalline materials exhibit significant physiological effects in test 'AilimAls. (This workis being carri_Q r the supervision of Dr th d/ 2. Complete fractionation and purification of crude concentrates which exhibit physiological effects in test animals.. When pure crystalline substances are isolated they will be identi- fied or structural elucidation will be attempted. 3. For those substances, which exhibit significant physiological effects and which have novel structure, an attempt will be made to synthesize them and compounds with related structure in order that relative physiological activity can be deter- mined. the rece was revealed that (L.. as isolated three indolic compoun e . - -rgic se � rem ivea Corymbosa. Also it was learned by private conversation that research esms of several of the countries leading drug firms have isolated flavanoid glycosidee from Rivea Corymbosa. The structures b obaan deermind nor has en publishe other an at b in in 19 s a result of these us emp.asceps= � ected toward continuation of this investigation. Dr who as a � worked on this pr if located at d plats to complete structural elucidat on � n our laboratory from Rivea Corymbosa. If it is possible to obtain a sizable quantity of Rivea Corymbosa from an area ingallai where the plant is used for medicinal or occult purposes, then it is proposed that the sample be carefully investigated to ascertain if it contains indolic compounds or glycosides significantly different from the samples collected in areas where the plant is not utilized for its .physiological action. 41. 4116 PROPOSED BUDGET FOR FISCAL YEAR 1960-61 November 1, 1960 - October 51, 1961 Research Fellowship (a) $200 per month Miscellaneous student.help (a) $1.50/hr. (400 hrs.) .Stipend for senior investigator (during summer) Special chemicals., natural product samples, and . supplies (expendable) Special analyses on research samples Travel and communication in connection with project Special books, rePrinte, manuscript and slide preparation and translations related to project Capital Items: 2. Matching funds for photoelectric attachment for Model NO. 80 Rudolph Folarimeter 500 3. Components (sweep unit) coils and probe) to adapt our NMR for fine line work in connection with project 1400 $8100 Indirect costs (15% of request) 1200 I Total request in renewal of grant $9300 Signed/ Swat of 246002Auree Ibisadber 20:29,9 October 32. 1960 T have e=mined and 2.71:.o ' the sultl:ad 2 cortgr that isto abr. arbottnerst of sompues ,xpendituxea. Di-1 ion �ot glum= Wasr42411 10A0CP *eta 471100 I tits699$ -visivr�riroar arlso`Tr$ on zapqa4..no Gomm eaztt4117039,b2 Tir4A1 9L4ligfit VitetferiA0 00*1.6TIT quiecipbx 6i�OtesT stasmAgt trantrarts 00�606%* (WITRINIATtaat guTtratouT) staaa we sovavres 096T 41S aecto'4410 � 6.0T gT asx14Trawb*0 WIN :ssiannptusxbizt :50i001a 101:01,01:0 9L-662.9 11P1+1,40 n 777- �Deport of Itcsenditures amber 14. 1959 � Oatcter 310 1960 LIU tt'd .v44'e S N A. t112g3.57)wae liced for $1,487.50 b.ing held project in the near future: memo dated 21. August 1959.. 'will be atiLiaed on another Salaries &Wave (inelumnsftllemehips) 04960.00 Supplies and Inverses 334.24 Equipment 1,294.63 Overhead 1.590.61 Tntal I have examined and approved the sOmitted expenditures. (rale TSS/Chamioal Division Date: .2,t .1".� 11 Ui July 30, 1959 Attention Executive Secretary-Treasurer Dear Enclosed is the financial report as prepared by our treasurer on the grant which, your organization made to. Dr. a..11111.11 The report covers the full period from November, 1957, to June 30, 1959. We will be happy to furnish further itemizing should you so require'. I am happy to report_ that considerable progress is being made on this rather difficult project. Dr. -01141p ,has two Very promising doctoral candidates assigned to che problem. They are devoting a-great deal of time and concentrat 1:Ve this investigation. We are grateful to the- 41111011.1111111.111. for the fine support this or has given us for this particular investigation. �� Silaries- end Wages Supplies and EXpenses Equipment Total Expenditures I have examined and ap7roirsd the submittal expenditures. These Azad st,re rireoptis .Cootribilt.tOU-�for tbs. ults of your . director.. In carrying out th. very 4.OrtItioslitte roliarets toils of your.orsaalsatiou.. � '� � � . ---,....- -. i � . CONFIDENTIAL FUNDS POSTING VOUCHER VOUCHER 0. , 1 - DATE . I- II - - ..� � t , :. � VOUCHER NO. 7- 1 . � DESCR I PT I ON� ALL OTHER ACCOUNTS 11.I$ 34'S* - 13. c.% - 44 4752 11.70 7 T ,� � DESCRIPTi0M- .. -33 2,A 0 ID. . '' STATION 000E . 40�43 EXPEND OAT PER: DRUG. REF. NO. 33 � .4.47 � GENERAL 'ALLOT .. OR ACCT. NO. COST ' � , ' ' � DUE DATE OVANCE'ACCOUNTS I +17 ' ' ''11/* Ill' i . ra.P' E". CODE - Lb. IS' Mel - CA LEOGSR., �� II 2.17 - . , , , ..... PiteJXCT NO. ,FY � CODE 06,p. No YR ACCT � NO, X CL 4* � , , � s a 0 .S. I s i s � . 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C...-.�i't . .. ;. . 1 a : .. . . . - � �a51 .. .. i . .''' 114 , - -1 1 - a .. a�� 1. a 41 I ��14 1,-,....; " 11 � a : r/gipv- .7.- .. i, -1 :�-n.t.i.,,,,,o .�4,- a .,-.. i' � i .- t ' .., � i � �� 1...1 I 0.� , � li. � _.1---. 71 6- .:. 0 or - a .3. �-��1: : �:. .- . �� A , ! .L - - � ... e* ' ..- . . - -.. 11. : - � t � .� a� -.. . � - . . - 'ft- . .:; :-.7r.:5 i'l-,�a`rif. S: a , a 1 4 � a- a , � .'.-- - li i a i 5.!,:r 1'7..7 a.' �' .1.1,..11- . �.' ill 9.* ir; ..r .1' 61.0,5 Or � o�,, � .f, .j, . -� .1 -s 1 - . ' ' � ..7�� . = - a .: 1. 9 6 ' -� � 6 - - . :. -- - -..! � , =� ; -. ....._ � tf.:,,, ..p. 1.'"f , .' �: : ,i's .1.` ... 7.-: � ; ,t �� +:�,-..a., .. .4:, � 7.. s? -.1e.... � . a� ' a � .7. ,� - . - � - . �,. .1 i ,.. 41 � ' � ." If ,41, � 6 �.�64. � *4 � a, t � e - 4 - f ., . . .... I" V" s'.'s � ' r - .. � 1. IN �,. � 4 � .. � � _ .1 -.�-�., 0 74 . ir" � � il �,;:� :ir ".� -� , � , . 1 " i. �� ' . .4-�" � � '. a - -: ... 4 n ��.:. l' - ' � -1-- ;4... sse ,,,, 4; -T. . � , .., ,se,ti k!.. � � , � t ' , 4 �*". 1 _0..4' � - � t..� ;Id ,��,� .d� #, A:41 il a �� i A,..:A0 .1., 14�43 � � � ,th...4 ��_. -1-ii * � .' a a � & . . - - 3, A, � a. 1...A� ... � a. � 3 � i y. '": A t . . " la r � . :', � c - 't -, A ,.. - � - .. ' a � t . .. - Wi.A.400 1 I/MM. , �� ' r. 3 ' Zt ' ji � � - 4 TOTALC:. .. - A.,:.t. ;at: 4 .. .. .. ' � , . . . .t. ... . ...: --7. ".-:* -*''' - f'''I' zi:71:`,* - . .- .! ' , * v, P= -""4-';� . . .. , �.:1 t: . � - X . -... - -3 10 . � . �. � -S.'. , � - - ...- r '42,1,%�;.�54 4 .1- �!�1 - . .-.. . 2., � . . -, , T' . , � r . ... -,,. � . -. ''- . . . - . !." � . . DAT . , ..� SPADED DV' , DATE '.� .'' . ... PIEVIlall" �11111'.' .. _ CERTIFIED FOR PAYMENT OR MEDI -.* .' ' - . " , . , . � - � � . - � DATE 'i.,' �� 1 11 MOTU* EEDT IF 1 � . , )` tali hereby cartiiiiild that thit teiroies es to'hiltlt.� =RA., , $sbprojct 22. which *as duly approve4 and that the projectli " bsing carried Out la accordance with the oseinersOup dated ,13 April 19530 brows the DCt to the DDiA.� and the eiteSilion at this authority oubsigesat soisnorticla.. : ' ont.T.irc..A.T. It is hereby certified,lbst ,*111 lrtm"coma.�"4� jeruntious ri).,). .41/Ctii.41',...11_ ...�1_59_1. ba, e been. satisfactorily �noispleted and v . _al 1,?!.. _z_lowsr weiveTr_. , g'sav ofeie 2t; which La still aantinatas also safaadad aloaap. 7b r" '''''': ,. wielnested that the ireininte shown below on invoice No.. le of sub.!, - . .., project 51,21 1:4, cr- *di tad to the subprojects as .shown below: .., - , ,�-.r,� �-.-.. ..t,.. I. tiiriA . .: It' OR' ..', .;,-., Anotaiiini (a9 jtme 1959) AP- MIDIORAME4 FOR: TEE RECORD SUBJECT : Project MEDLTRA, Subproject -22 1. The purpose of this Subproject is to provide funds to continue the research of Prof :a ' the isolation of .111111111111r compounds having psychogenic and. related properties from natural products.Mis concentrating his studies on Elves. Corymbosa variety and. Amanita Mliscaria and has isolated some active materials which are of interest. 2. Difficulties in obtaining properly documented. raw materials in sufficient qUantity for further research have been to a. large extent overcome. Considerable renewed interest on the part of several drug companies of foreign origin in related fungi and plant materials believed. to have central nervous system activity indicate that there may be chemical cal:pounds in the natural products under 1-50 study of considerable 2EXt interact. 3. tatallaraggaillagillakall will serve as cutout for transfer of funds to the investigator. The estimated coat VA�11301, for the coming year of the work is $21,547.00. To this it be added. the 14 service charge to the cutout: thus the total cost for one year ' wilt not exceed 412929.68. 4. A Memorandum of Agreement has been signed. by the contractor whereby title to permanent equipment is retainedbi_tbeybiversity in lieu of higher overhead Charges and. arrangements are made for accounting bursed under the grant. 1.141, APPROVED FOR OBLIGATI= OP IMS: Budget for One Year Renewal of Research Project on Invite art � Mnscaria and Elves Cbrydbmme. Nov. 34 1994/Cts Research Fellowships - two. (a) $200 per month Senior Investigator - during stmaer Capital Equipment - (Electric desalting apparatus, explosion proof bomogenizer, vacuostat, sPinning Pallor � chromatography apparatus, etc.) Special Chemicals and supplies not ordinarily stocked by Mepartment of Chemistry ........... . . .. ........ . .. . .. . .. . 850.00 Special analyses on research wmnples 300.00 .10 overhead �� 11507000 Total, grant request $12150.00 P- Arg'..4Zr ',04A 0 0 /1'14 d I / C .arozweak�4,1-1-7:A- , teal FCR AYMENT OR OMIT � ,ING.,OFHCE -v4,44q: ) It is hereby *tined- that this is Invoice No 8 applying to =ULTRA Subproject 22 that perforinanCe has bean satisfactory, that the services have been laconiPlished In accordance with mutual )77. agreements that a detailed 'agenda of the payments and recliptk is Oft file in TSS/CD,.. that this bill is just and correct asul.that pay- . mast thereof has not yet heen made. , � " Chief. TSS/Chemical Division , � fair... ., . . MOOD ..... . , .. MISCELLANEOUS OBLIGATION RECORD . .. . _omit' , .... . PSOCAL YEAR ALLOT. COO( NO. OOJEOT OILAOS�. INSTRUCTIONS: Proper* oritinal only 5 9 .. ' . . . ... �.. .,,.., .. ,,.� , PAYMENT DATA NAME OR CRYPT� OF PAYEE (Ind/oldool. Pita. 0100 '' ,-, . . .. �.., � 1. . . PURPOSE OF PAYMENT ,, � � -4 1 , Me .... .. ;S: ,... . � 4 . . .,, . . PERIOD COVERED . . MITMOD 07 LIQUIDATING OOLISATIOW DATE OSLIQATION INCURRED CONTRACT NO. ON OTHER REFER. SIM4LE PA7MENT .7 .ENCE. IF ANY L I IRREGULAR PAY NNNNN EST. DATE OF FINAL PYM.T. , . REGULAR MONTHLY NNNNNNNN DATE REMARKS AND REFERENCES � OELIOATIONS INCURRED OSLISATIONS LIQUIDATED UNLIQUIDATIED SALANCE ',- . . , . . , . 7.'ep , d Now 3119 A* . , . MX OV ,...... . .. aft"(( .S944 444* ....... i 431 (-2-�44,142f.&0 414,- /..2 . 3W a ., � , - .-. .� , , � . . . 5. r , , , _ . � . . . .. _ . _ FORM NO, 461 REPI,AC the authority pastel to the tteaoraohlo *Oat . atifsiodeas, 3953: te, .� .1111 tiegamilase t tabut tosaa o , � � the er�r!..11 ink** MIN* bola has Ulla the eafiskisette saeloose aid should to stars* . , CERTIFY THAT FUNDS 'ART AYAICABIli ' OUNIATION RUIRIMI No, Yee..? ONO 7%1 ALMTA:air No...ttp�,04 � $Y2 -1 ). 1. ,I920 !ink, �0 2: Tflf, 30 October 1930 Dr. This is se invoice ags-thet PrevicuslY obli.gat.ttd. � of. ObligatiOn: 30 09t0er,195�_ 44 � PIII"X)041. cr ' Extraction of Natural Products. irsticia >stmz titri.tr,or: 1874 orit %Juno TY'DETI. CL BY MEMORANDUM FOR THE RECORD DRAFT 23 October .1958 SUBJECT : Project MKULTRA, Subproject 22 1. The purpose of this Subproject is to provide funds to continue the work of Prof. on the isolation of psychogenic com- pounds from natural products. Prof. has been working on Rives dio Corymbosa and Ammaita Muscaria and has been able to isolate a number of interesting materials from these sources. 2. Progress in this field has been somewhat delayed due to diffi- culties in obtaining properly documented raw materials; however, sources have now been developed which should correct this difficulty and make possible the completion of the project. The recent success of the Swiss Chemists in isolating and elucidating the structure of a new psycho. chemical from a fungus indicates the feasibility and the necessity of continuing work of this type. 3. The will serve as cutout 8 for transfer of funds to the investigator. The estimated cost of the work is $9,867.00. To this must be added the 110 service charge to the cutout, thus the total cost for one year will not emceed $10,261.68. 4. Prof. has signed the Memorandum of Agreement whereby title CI. to permanent equipment is retained by the University in lieu of higher overhead charges and exrangements are made for accounting fo,r funds disbursed under the grant. Dovngradettl)t.kowstimt Irt authority of: 187475 date: Juno 1977 , 4 .........�................................. E2 I1STZT; CL BY 187475 5. It has been 'mutually for travel expenses under the egstit which are re University shall conform vith the accepted practices of that Institution. 6. Dr. es holds a covert claim's* =der Project NCO R A. and has agreed to comply vith the terns of the Memorandum of Agreement. Attachment: Budget APPROI/ND FOR OBLIGATION OP Date - r /ciiT. Distribution: Orig. only wng:rndedtot*SIREINt authority of: 137475 3: June 1977 NOM .1:21)::T; MI BY 137475 BUDGET FOR 195940 Salaries, two pre-doctoral fellowships, principal investigator and part-time help 5,460.00 Supplies and equipment 1,000.00 Special Cohonli cals and materials 500.00 Special Analyses on research samples 300.00 Travel and communications 400.00 Library Service 100.00 8,760.00 Total for basis of computing University overhead 7,.264.00 15% Overhead 1,107.00 Total 9,1367.00 WARNING NOTICE SENSITIVE INTELLIGENCE SOURCES AND METHODS INVOLVED UN rry, Di'. July 1, 1956; througA Oct. *cot 570 -- 'ia1 Report Balance, July 1, 1956 (Per prior report) , Receipts: August 31, 1956, 4th quarter January 31, 1957, lst quarter March 18, 1957, and quarter jUllia 24, 1957, 3r.d, quarter $2 730:74 1 325 00 1 407 SO ' 1 437 SO 1 Str 50 � 7 518 24 Expenditure and charges; . Total direct charge3 per prior report, 2/28/57 � 4 ZOZ 46 , Salaries, 6 mos. Cu 150.00 �-' � -- 900��GO � - Travel el.-pense, D.11110.01110. 30 00 Telephone and telegraph 8 (").T, Supplies and MisceLlaneo%ze 145 :77 Total Direct Charges .5 236 C6 " Overhead, 10% af Direct Cot a . 528 61 5 814 67 ..Y111%��10...� �� Unexpended Balance., Oct., 1, 1157 $1 7Z * Check for 41. 703,57 to co,rer refund of unagr.encled baltAnc3 fol.:tart:lad to Check *0 20251 drawn an thedillinai1le1011111IM1lIIIMMONIIIF $16,819.00. Check *DM 43521 drawn en the 0. for $672.76.. Your generous contributions are greatly appreciated. The funds will be utilized by our Board of Directors in furthering worthwhile research projects in the field of medical sciences. 41111101 equest for renewal... or, 'support for the' projects jo Thve�tiation of thel"seiid of Riveirporrisbassgoloingui)� ssummary of work, November 1# 1955 * September 25, 19561 Physical examination of the seed used in this work reveals that two "types" have been under. investigation. Taxonomically all the seed have been called Riven CorTmbosa although one variety (the seed are the larginirthe two varieties) originated. InAlexico and one variety originated in Cuba. The literature on:the subject of olthliuqui, as far as I can ascertain indicates that its use as a psychic drug has been restricted to .Mexiao. Thi a. alone would -indicate the probability that the Cuba 'lacks some physiologically active 'component .lch lexicon variety contains. Experimental work this la ratory � has been largely restricted to the C an vane (ommill� seed since only a very small amount � the :1ex n variety has been available. The chemica work, alth not deoignetto.compare the cm.. f varieties, has indicated some dtfforenJ in th we. 41so physiological tests on humans are indica k femmee in the two varieties, This work is -- .olusive to be =oh more than speculation at In addition to ol glucose and hydro* quinone as constituents olo uqui as reported in the' last progresa report vstalline fraotions have been isolated which seem be alkaloid or glycoside. Die to the small . eed available the amount of these crys tall frac is extremely small and little m e than qu e tests have been made on them, Experim teobniqu * especially in extraction and aeparation'Save been perfected which will be used to isolate Pu iu.1.itt.tS of the drug which, just-today, has become a ilablo in sufficient ,quantity to make the ieveatigat 0 3 profitable. Plans for e investigations Now that 50 pounds of the Cuban variety and 10 pounds of tho :lexicon variety are available, the following lines of inveatigation will be actively pursued, �' 1. Determine conclusively if there is a chemical difference in the two varieties of seed. 2. obtain aS many "pure" compensate from each variety, using previously perfected toobniques, as is possible. 3. 6ubmit for physiological testing ouch "rare" component. 4. .:11.bjoeit each "pure" component to chemical examination to determine the typo of compound, whether it la a known eeilpcund and its chemical compoaltion it it is a new colpound. -to � :Equipment(including larger scale �-extraction and separation equipment) . 1.and special chemicals 1000.00 �;Anal7ses and tests ...;........ **** sesiless 350.00 Travel expenses 500.00 . Salaries lone graduate asst ant lab� t0r7 help and director's stipend ... 4000.00 gi3 c a i lane cue � � . . 100.00 Total 45950.00 VIA s TSS/Budget Officer � SUBJECT ILICIA�TILASUbproject 22, Invoice No. 7 . Allotment 8450240401 " 2. The checks should be forwarded to Acting Chief. TSS/C;henmical Division. through TSSiBudget Officer, no later then :Friday, IS October l957.: 3.. This is a Biel invoice A total of 817,491.16 was obligated under this subproject during FT 58. However, since it La anticipated that additional funds will be obligated for this projeet,', the files should not be ilosed. � Acting Chiif emical Division Attachments 2 Invoice it Certification, Distribution: & 2 - Addressee 1 - Comptroller � MEi ON 71 - TSS1FASB . 4 , 1 , ....4,.. I.,. � � Cehi.ra Clak dtd. 22 Oatobei: 1957, Nes 02025, in =emit 40.6819000, end cashiers Cheek dt4. 22 October 341aala43521., ill =Mt 461246 flit t 957 Begistered Bat. Rae '" tots � 1..e�-� iwo obis. *Iwo. idi:ii.44�01 rear* al .401. 41. aim . aortre;iir.r;4-0.'40"0%arilitaiiwiiiO4.10,0, wars* .. .. ..441ed TIFICA.TIO 414 9t-4-?!.,..' =it is har.by ceztlfl.d thatibia ie:inivice No. g to Ititt/LTIL.A &Apr that perforancs haii;h4en. sitiefactOry. that th4. earniconspliehed in 'accordance with mutual . � . i.agisoarnents.;. that & detailed agenda of tho.Payniente and receipts is on file In'TSS/CD, that this bill CS just and correct and that payment - thereof has ant yet been made' Acting Chief, TSS/Chomical Division 4:0 � halvah's!. ad this iPple � to ii*ULTRA and that the project Is lag carried out In accordance with. the memorandum dated' April-1953; froM"thi DE= to the DD/A4' aid the tension Of � , authority in subsequent'snamoiandas �,,P 'arty. moolOtNamowar...% tIlea :;;�9 --Tiad to: h rl.tY : e 7 ; CL BY 1 475 w 8775 100 9, MEMORANDUM FOR: The Record SUBJECT Project MKULTRk-SOProject 22 ' - 16 August 1957 � , 1. The se subproject is to provide funds to continue i the work of Prof. on,thlliolation of psychogenic cam- pounds from natural pr uc s. of.111111Fhas been working on the seeds - of Rives aumbosa and, has been able to isolate a number of materials of potential interest in this field. Downgr 7 June 2. With the advent of feasible xatory methods for testing psychogenic types of materials, Prof eels that he will be able to characterize the previously isolate Materials during the coming year as outlined in the attached proposal and also fractionate Rhynchosia phaseoloides for its active principle during the same period. i111111 located at University at vers as head of the rtment of Chemistry. This cge position will _ any way interfere with the Agency sponsored. work, in fact Prof- 11 have epa 04.C. access to more and better equipment and personnel for his research. 4- The will serve as cutout for transfer of fun s .,-- ga or. Yie estimated cost of the work for a period of one year beginning 1 September 3.957 is $16,819.00. To this must be added the 4% service charge to the cutout, thus the total cost for one year will, not exceed $17,491.76. 5. Prof. has signed the Memorandum of Agreement whereby title to permanent eq Is � or nt is retained. by the University in lieu of highe4 overhead charges and arrangements are made for accounting for funds disbursed. under the grant. 6. It has been mutually agreed that documentation and account- ing for travel expenses under the grant which are reimbursable by the University shall conform with, the accepted practices of that institution. rit' 3. Profliplvss previ Be is n o ocated at 7. Dr. holds a covert clearance under Project WOLTRA and Cl. 37.1 � TA2 PDET; CL BY 87475 r147'-'eded �.thority of: 187475 June 1977 IMFDET; CL BY 137475 has agree& to comply 'with the termA of the Memoram&mm of Agreement. Acting Chief ,TSS/Chemical Division Attachment: Proposal APPROVED FOR OBLIGATION OF FUNDS: Research Director to,71W*WhItAtt. )7 authority of: 187475 ez: June 2.977 CL BY 187475 .V.,"�-i- ,..; %,---..f.,.,-.--..,...;-,-.--...-..5.,..,..------5.-...:,,- ...,-. ,-,..., . . , it has been ascertained' that there are at. leas o s.types of seed from the plant identified as. Rivea boaa* ' _ One is found in Mexico the other in�Mrs".,. appereo.t composition. and physiological action --- organisms _differs. - ---*. ' --..,-,:..,,,--.. . ar�-;�-�: .i.:--,-- "Y- --- :"i:14,-.:t;----�-:'-- 4 --.1'C'- � z1.- ,- Prom bath seed, glucose and hydroquinane have en isolated and identified. One additional compound been obtained in crystalline form* apparently ThIL-compound is being studied, _ The bulk of the drug seems to be a glycosidio mixture, This mixture , b.as, to date, been subjected to numerous methods and tecbniques designed to separate the component parts, 'None has proved succesaftil, The mixture appears � on the basis of many tests* to contain a small amount . f alkaloids it is not known whether the alkaloid is �-integral part of the aycosicle or not � � rhoVS iologitiale tests 'Using animals and h�znani have - eel; inconclusive-. and confusing. *- Enough positive sults have been obtained, however, to point out the,. for more,-carelttlly designed and controlled tests 'WARNINL-i NOT!CE SENSITIVE. 11,NTELLIGENCE AND iliETFIODS INVOLVED ' SENSITIVE INTELLIGENCE .., SOURCES AND METHODS INVOLVED Cashie check No.. DM '41928, dated June 12, 1957, drawn ia*Wrritil � 44,84/ Ar,,,"1.$1,V=T4,441".! RECEIPT ' Receipt is hereby acknowledged of the following two checks: a urer's check No. T63248 dated J 14 1957,, drawn on the the amount of $3, 96 pa.ya 4431111111* on in the amount aanrariiiluiek 83248 unt, f4, 1957. drawn =nun "Irra14.:7212 n.A....W .. 1 REMAFXS ' KC - REELNCiS... - ihrOATICNS CBLISLA.17�arS 1�ZILISar.01= . � . . nr.ARM � - :LIVIDATED� :. : BAL'Arr' " � � DEC . 1956 -- - ' �:''i - � ' , a, ....-. :-..:.:4;.:...,t; lici,... ,- . : t......, . -: -:,.., ..�:...i, . ......: , -. , �. � � itre�0 ,........,...tece evo . 4�21.95s- -424.4�,,.t.t.� Aro/ :444 � 74. ts AZ, � �, r - , ,10. . azi' .7:,..�4�. 6 . , . - .� ,..____ ../...A.a 4/42.17.5 .. # i I , - , If. . i - ., ..7.:.- - . . . . ,.. .� . - i �.. i _ . . � � .. � , . .. ' ,,� '� ' � et - .. . . , .. - , . ' .. . - ' � . .. ._ .. . - , ...............................A..............,.. ,. ' 1 ..........................�... 1 ! ... 1 i ' A � . , . . . ' March 20, 1957 M.D. Di,e�ecto Dear- Dr. Enclosed please .find in duplicate our statement of ":�-: � funds on Alkaloids of the Drug known as iTiulen for the period from July. 1, 1956 through February 28, 1957. The balance shown thereon is 41, 406.82. We thank you for: your interest in our projects. versity WARNING NOTICE ENSITIVE INTELLIGENCE ' S AND METHODS INVOLVED � 3e-...-,-�-�;e.1.2.-�-. ir2/.56 .'" ' .'4.c.), ,� Oci.:bT.�?�� 14-56 --.� '19.03. ' .... �I/o-sriczer.l.F55.-�� ,2.3.9 47-- � 4.63E,P4- o:. ..4,9.'s � pik.. 9,861,1;. lb.v.,�7e7-412.,taima.:,...:,;iz-tri:,�,:r:. ':%':-t,.--3:1-;:�:15-5( eic.-109�32'r . . ... . r lb. 75'.0 -. Bilt4t....�. A 7:arc; r !I ..,,.-7;, ::� .,.4,,,:-'.. ,v-ri-r9,1;i3:;.:7�,.:*..4.;;,., , : e.: ,!,.. �!..p:.� , ,....:- , r.r. .... . ''',1--;-- � -1.0r:4.' Clr'''''' .1..?(IY7'1�. �40 . ' I. . 11111111111111111MMIOROPII, . ta....v......�1.,...........e.,".... .....0. � � ',1003 ,6,11-1T-22975,1�C�alltiloze3-..Powder..k81clit,s4.:St cl. ,J., ..-. � � � ...c,v. � 213' 19...),..>� � ��Al.c...2-.(23.50:3 . /0(0 2�I 2'J:.- 1 itio- Ai -^?....Dar, z3 ,a. Al..?,,,r 7. ) -I of() � P7.- 1 9.111, C . IC ...... p, L.',..) .." .", ......�,..i. ..,...41,,,,,,I. 1 .. /.;:' .0.1).7..r'1 ";��;.,113. ,,c...,?.Q (.1E4 4,- � .1:1A31:cal Tr-:�:.,f....,::::. fs:nt .6:1 , nx.' .* . :7,67f. 27-: 3.956 Cli. 12LC.0 'L'er,t .5-CT�CC:. :'.. � , , . -� �-��=, : (":!:.- --r r11:*-sC4,; '..:1'..`'..9.1'.! "1'75' :i222 II. '; :. :-.:.-.1.0.00. .....c.v..... ,,v, .... _ - 1- 1.165::-.::::,;:".� Pi.-.?rv�-4.) 2. t....-:: c!...7 C..... 70 ...3,:.0 ..1.1iii;:r ...1'.', ..':: '2.1;132 ' .... � ' ra400. ,_ -..,`..) A ....i.07.11. "�t...On01 ,'!�, '...'''. 5 g�?.1. '.�9'.1; .:so i�..--..t);,-,y-1...V10,;.111,01.1..t !Ps 7450 � ' � 31''''',3.3 7's.::75.7r7,,r..3 C:-.21 Z.,3?-2...:.',.�?; ".1T,r 32.�' IZ 313� �'3.35 ' =11 71^,:::;,'..,' r.7.. i, .........., st _ ,.,'�:....". .....�; �4..- ...�.1.,..,.. .......:/...,�.......:,... -.'i.. tf',:a::sr.'.117-J2.) 74:2..';.-:: ) .71-Gla 24414 411 "717.,:17.7rYr c:,�;'.. .s,-,,,,-�:..,.v.:.1 f'3. (-11 1 'II - ''��137.3.= 0:�. ii!".i.5 i.� .::.f e, tcp r.:',.,-. , R.., 50 11.:-.:' A." ' 'S'inrCf..71.(Celite) A 3040 2.00 oho J7 -c.k.:� ljah,C vtorIc 0 2tt7tis3C .z;ri vainples 'cook b,ob 3.0 0 cb.23, 19T Ck 4.1-,,:121. . co Evz....p6tor, .� � :. dal....)isiTztts,l'3:mats--, ,.�� '44 � ck: 14292c -.1-17220,iBleridor BaLlzica in Fund F�a'ern,.1.4-:-.; 281.'195:7 � deeMet,'c1f.c.r" 30,78 Add r.�03:ipt 10252; 12arch 1$32- 1957 � 1wa7.5o , 2..,827.28 Receipt is hereby acknowledged of the following two chicks: drawn on th dated 21 Deem er s check No. 147713, drawn on the dated 19 December 1956, in the amount of $79.33. Both checks are payable to the Inc. Invoice No.4 is attached covering the above subproject. � �-. "meat should be made as foltows; Cashier's check in the amount of $1, 983.33, drawn on # Cashier's check in the amount of $79.33, drawn on if 2. The checks should be forwarded to Chief, TSS/Chemical .'hrision, through TSS/Budget Officer, no later than Monday, 31 'ocezntler 1939. Other invoices wilt follow. Ce tfleation* CHECK4ietaaN TH5, ANIVINT ar RGEIVED. ) It is hereby certified that this is Invoice 4Napplying to MKULITRA Subproject 22, that performance is satisfactory, that the services are being accomplished in accordance with mutual agreements, that �� a detailed agenda of the payments and receipts is on file in TSS/CD, that this bill is just and correct and that payment thereof has not yet _ been made. (2). It is hereby certifted that this invoice applies to MKULTRA Subproject 22, which was duly approved,- and that the project is being carried out in accordance with the memorandum dated 13 ..Apri11953, from the DCI to the DIVA, and. the extension of this authority In subsequent memoranda. Thule, thi'authoritY granted in 'the memorandum dated April 1633 from the DCI to the DDIA and the extension or this authority In subsequent 31301,120141114144 Subproject 22- was previously " approve& tkider the &arse asdhority sitradditional suns of IL 188.00 hes been -authorised to cover the subprcdestts expenses. ICERTfIr'l Atr 0:21?r, osti'�ooed� F.:.�'7" N.. � ape..� 4. pio..4st Atni�C�tiliNG'*:- . ' -- .�; ..� Dorrigradod to: by authority of: 187475 date: June 1977 E2 I!ZDET ; Cla )3Y 187475 ��-� MEMORANDUM FOR: THE RECORD DRAFT 5 December 1956 SUBJECT: Project MKULTRA, Subproject 22 I. The purpose of this project is to provide funds to carry out the isolation and characterization of the intoxicating substances present in certain varieties of RiVea corymbosa..8 2. Dr of11110111University has conducted studies on this material which have lead to the development of methods which can now be applied to large samples of seed, thereby allowing the production of sufficient quantities of the active materials for definitive biological testing and characterization. Dr..et has submitted theC attached proposal to carry out this work as directed by TSS/CD. 3. The serve as cutout for transfer of funds to the investigator. mated., cost of the work is $5,950.00 for one added a four percent service charge for the ing to $238.00. Therefore, the total cost of this year will not exceed $6,188.00. - will 13 The esti- st be amount- investigation for one 4. In accordance with the Memorandum of Understanding with Dr. 111$161, the University shall retain title to any permanent equip- ment in -lieu of higher overhead rates. � 5. The 0 1111111111111111 has made arrangements for the re- turn of unexpen0 e monie1s received under a grant from the Fund and also has requested that the University submit to it a summary account- ing for all monies.received therefrom. 6. It has been mutually agreed that documentation and account- ing for travel expenses under the grant which are reimbursable by the Univ ersity shall conform with the accepted practices of that institution. � ,� DommE,Taded to: ANAL by authozqty of; 137475 :une 1977 � L:PDET; CL BY 187475 Omnraded t. ,sr autht4ity of; 187475 late: Juze 1977 32 MITZI; CL BY 187475 7. Dr holds a covert clegrance under Project MKULTRA and has agreed to comply with the terzAis of the Memorandum of Agreement. Attachment: Proposal APPROVED FOR OBLIGATION OF FUNDS: esearc. Director Date:� Ns5c; Distribution: Original only Down:;raaed t� b7 a-4thority of: 187475 .111:19 1977 CL BY 187475 Chief, Branch II TSS/Chemical Division Approved: r, ..� enewal ef support tor ..4; Yi;:r' ' *4 Investigation Of the teed of Riirea Corymbosa(oloiuqg � eqUes � - Summary of work November 1,1955 - September 25,1958: Physical examination of the seed Used in this work reveals that two "types" have been under investigation. Taxonomically all the seed have been called Rivea Corymbosa although one variety (the seed are the large17-3T-the two varieties) originated in Mexico and one variety originated in Cuba. The literature on the subject ofololiuqui, as far as I can ascertain indicates that its use as a psychic drug has been restricted to Mexico. This data alone would inchoate the probability that the Cuban variety lacks some physiologically active component which the Mexican variety contains. Experimental work in this laboratory has been largely restricted to the Cuban variety (small seed since only a very small amount of the Mexican variety has been available. The chemical work, although not designed to compare the. composition of the two varieties, has indicated some difference in the two. Also physiological tests on humans are indicative of a difference in the two varieties. This work is much too inconclusive to be much more than speculation at this time. In addition to the isolation of glucose and hydro- quinone as constituents of ololiuqui as reported in the last progress report, two crystalline fractions have been isolated which seem neither to be alkaloid or glycoside. Due to the small quantity of seed available the amount of these crystallind fractions is extremely small and little more than qualitative tests have been made on them.. Experimental techniques, especially in extraction and separation, have been perfected which will be used to isolate pure components of the drug which, just today, hts..1 become available in Sufficient quantity to make the Investigation profitable.' Plans for future investigation: Now that 50 pounds of the Cuban variety and 10 pounds of the Mexican variety are available, the following lines of investigation will be actively pursued. 1. Determine conclusively if there is a chemical � difference in the two varieties of seed. 2. Obtain as many "pure" components from each variety, � using previously perfected teChriiques, as is possible. 3. Submit for physiological testing each "pure" component. 4. Subject each "pure" component to chemical examination to determine the type of compound, whether it is a known compound and its chemical composition if it is a new compound. ec ',,..,VARNING NOTICE ,:i�IsITIVE INTELLIGENCE AND IYIEIFIODS INVOLVED Proposed budget November 1,1956 -November 1,195'7. Equipment (including larger scale extraction and separation equipment) - and special chemicals $1000.00 Analyses and tests . 350.00 Travel expenses 500.00 Salaries (one graduate assistant, laboratory help and director's stipend) � 4000.00 Miscellaneous 1 100.00 Total $5950.00 WARNING 1\10TICE SENSITIVE INTELLIGENCE ,'JIRCES AND METHODS INVOLVED eceipts: 79 72 n 325 00-- Total Szpenditures Balance in Ftuad: june. 30, 1956 (for. 2 quarters) May 23, 1.956 Receipt .A 30002 - fat 3rd have examined and approved the submitted SIDNEY XSS/Chemisal Division \fatlanents Insurance 8 84. Or, 5023C 'Carbon 7 y.o3.. Vit. (1) Special. � dc 5278C ,1-The .411-aloics Vols. ek 5340 rricals &2pM. Telephone CaiLs Rec. 30719 Reirbursersnt for bree14.ge (claim.) � P/C Pne Ca3.1 pmen dc 6726 Cs.,lit 1'1 'Infra absorption Spectra 2 00 Standard Spectra indentification 2h 00 els 601 6:kwarimmumains1 � F1ax.411* tiagnetio Stirrer ...21.22.......211,1 siTr, �-.DLL =NARY OF OPERATIONS .3i.'].94 5-1-1955 4-1-1956 THROUGH THROUGH trROUGII 4-30-1955 3-31-1956 5-22-1956 TOTAL RECEIPTS: 7 700 00 Apparatus, Supplies and Expense - 979 72 .Equipnent 2 325 00 Total Direct Expenditures 6'174 76 0-,;erhead Allowance - 10% 617 47 Total Expenditures 6 792 23 4,- Supplemental iteceipt - NO. A 30002!7 5/23/56 Adjusted. Balance 1 704 17 170 42 1 874' 59 1 325 00 46 85 37 50 23h 35 23t3 257 78 8 924 60 1 325 00 2750 40 have examined and "approve tha 2tted oxpend�mes. SIDNEY GOTT ., 'Ctief TSS/Chemical Divislon JIM) 1101256 uviv las kat ivne Receipt is hereby acknowledged of the following two checks: Cashier's Check No. 302611, 'drawn on -the' dated A ril 26, 19 AUTHOREtZ.'0 CZniriT,F,Y1 � � , .;. It is herd* 'eartitied, that, thie is voice4lspplilng to NKIXTRA. Subyrojeot 22, that performano. is satisfactory, that. the*scrvices are being iccoiplished in accordance. with mutual agreements, that .sc details& agenda of the payments and receipts loon rile n 138/017, that thief bill is just ant correct and - We have your letter of March 29,1.956 relative tamnr jesearch grant made for the investigations of our Dr. It is indeed a privilege to serve you and your'pro ession in this connection, and �r oMission of ac1now1edgmentt and statements are due to pressure of work rather than to lack of interest or gratitude in this end other projects. We he ropared a' set of statements covert* Dr. peration of your project and. are enclosing .three copies. These,consist of ' a'summary of the entire-Period from March 1, 1954 through March 31 1956, supported by-two detailed statements covering the periods March 1, 19540 to April 30, 1955, and May 1, 1955) to March 314 1956. These statements show receipt from you of 07,700.00 on the grant to November la, 1955 and $1,325.00 on the second grant (5,300.00) to October 31, 1956.. 'Should you desire a restatement of the operations to coincide with the grant terminations, we shall be happy to make them up accordingly. , Tours very truly, , Vice President Auditor ,IS.ITIVE INTELLIGENCE AND METHODS INVOLVED sirc0 sunuar OF 01' A 0211:* ,��% NiRCH 1,, 19514 Meow; mARcH 313, 1956 1-1954 5-1-1955 through through 4-30-1955 3-33,1956 TOTAL RECE.T.Y2TE :7 700 00 1 325 00 9 025 00 Balanca in rund: April 30, 1955 March 31, 1956 1 079 00 3 719 014 230 00 180 00 410 00 14145 17 1 1.44 89 2 325 00 907 77 . 358 13 tte s%itl2tted OCte NCV. Dec. 1955 7 'web. Travel,- . :Time 1954 Ck ln.653- Dr. To meeting on ilkalo' id reSearteh 'Payroll Expenr---_ Mar 1954.37692 rsoriczents Ccz.insur=ra Aug. " JV956 " " " 1107. .31/1 206 Feb. 1955 " - Social se;crzity Apps, Supplies 2ncl137.7,29:,.10.-% March 19514 Ch9893F- extveztizing Che.cal eEngint,AertIng !Imo April 1954 Ck1C059?: empoggsmisio Tritmion cups & other fittings Ck. 10370F Laborztory equip-mut ir mattles Ck 7C438Y I2ay 1954 Ck la,C;;;.) Heatinv rt=:_ntel aad avan:nt C17. 1(1727C - Ch2micals Ck 1065CC - Intdr,1 timer :anti 1954 Ck 11457C cp.,374,, OW= 41.1.no 195'4 Ck 1033C11 exchsnges �11I Ft9L - Petty oash4 rr raid - Lo0 00 16 20 400 03 27C0 15 C�i ZLOO O CO 163 64 . 163-611. - 263 64 ' . 163 64 400 OD 163 64 , 163 a . . 11 70. 86 0:5., 86 ;1.6 2�,6 02 3.2:, 11; 27 3D F.C,ItJUISZT: n. 2 Chack 314:33 - 123.C3C- "- 1 ladel DK, Spcarrop1ats2.-#e:4, .Bacialait #9500 Less b.arne. by Chemisyn, Dent. (i) Ealancs Availabla in FunA st A ii 30,191.;-5 � / hare examined and apprc-7t,.t - -Apparatus, ..ftwlies Tiorimen,i Corvendation � ire1epho,..0 Hail"! � Print-Inc; Sationery 1 1956 Ck 38420 Theico �Vaeuttth. Overt�, .--tran. 1956 Ck 2775C INNOMMO� Scientino - 1 Indicatrz sprv:bottle � � � ��,..� .:,�Supp lie s Feb. 180. 1956 Ck 2833c. 24 Sublimation Tube* Extraptiori:'Asverab17-,. � Sublianation Apparatud #377550 . Feb. P32 1956 Ck 3191C - 1111111110. 1 Cold trap of P. tr ass ' � Feb. 27, 1956 Ck 3309C 2 Ileating tapes . 'March 15, 1956 Ck 3706C niectilar 'Weight �. Carbon - Hydrogen 1956 Ck 4374C � �Plate- glass rare , 1956 CI: 3707c - 1956 J�n. of Pharnaeo r."4-:erincnt31 Therapettics Total Fz.zpenditrxez Overhead Allor:ance 3.01, : Balance available inFun ...c043 e purpose of this memorandum of agreement is to Set forth the mutual administrative responsibilities of the principal re� search investigator and the sponsor. Therefore, it is mutually agreed that: � - � ..!*.A. The principal investigator shall submit to the sponsor, at six month intervals, an informal, statement of expenditures of monies advanced for research, activities. It is requested that the statement be submitted in essentially the following form: Personal Compensation (Salaries, Subject Fees), 24PIPment Supplies and Services Contingencies Overhead B. It is understood that any. travel expenses incurred in the furtherance of the research program will be accounted to the University in. a_manner consistent with its established practices and policies. . C.' The principal investigator shall submit_ to the sponsor semi-annual reports on. the technical progress of the research covered by these funds. . D. Title to any permanent equipment shall be retained by the University in lieu of higher overhead rates. I hereby acknowledge receipt of the following:,' Traasurerla Check #151699 drawn. on th payable in. the amount of 7J.tbd � ...7��-.."--�� '"'"."..........�"."..".�� oft � � , graded 'tos thcritilif: 1,87475. Jilne 1977 DETT. CL BY 18747 DRAFT October'1955. MEMORANDUM FOR: TEE RECaRD - - SUBJECT NKULTRA Subproject 22 � 1. Sibproject 22 is a continuation of Subproject I, which had is its scope preliminary work on the isolation and characterization of the alkaloids of Imoomea sidafolia Choisy (Rivea corym bosa) in order to determine their activities as agents affecting the central nervous system. 2. The results of the prelinivary study are such. that a full program of research on this material is desirable. However, further investigation is required to determine the best used in the purification of certain glycosides. Dr. who�: conducted the prelim/eery work, has submitted thIitTacCd proposal to carry out this work as outlined by TSS/CD. 3. The , will serve as cutout for ransfer o ..s o the i,es ga or. .6 estimated cost of the work according to the proposal is ;5000:00 f s must be added four percent service charge for the amounting to $212.00. Thereiore, the total cost Ot a VO8 gation for one year will not exceed $5,512.00. 4. In accordance with the-Memorandum of Understanding with c Dr he University shall retain title to any permanent equipment in lieu of higher overhead rates. 5. The 411111111111111' .has made arrangements for tii6 return of 0 111 1 unexpended mon es o e1 penny upon completion of the program. This agreement is maintained in the records of the Fund. 13 6. NIIIIIPbAs agreed to comply with the attached Memorandum of Understanding. He has been granted a TOP SECRET Agency clearance /n and is. fully capable of protecting. the Government's interest in this project. . APPROVED: esearch en Da- � Klqi5 Mitribution: Original Only ef hemica'vs raded t by tu t.;2orii.7 of; 187475 - ()Ste: June 1977 � September 1955) E2 IMITET; CL BY 187475 MILI=MS: 1.:ZriZA4M'APPor*- ALAX:?: Orli/ & 2 . -., I - =Mx TS/FA � MAD =WM *A (26 Soptamber 1950 My letter to the. on May 2 constituted a brief progress report of my work on the project dealing with the dru "Piul " e that time, arrangements havbeen iade with C:Dr. ad of e artment o at ,the in to carryov p1i9Ilogic91 tests on ,�e purified glycosi e o ained from "Piule". These testa will take a considerable portion of the lycoside obtained from the "Piule". on hand, however, Dr. of your. organization has indicated that he will make more of the drug available to me. When I obtain this new supply of the drug I plan to extract and purify a sufficiently large quantity of the glycoside so that when it is subjected to splitting, the aglycone which is obtained, will constitute a fraction large enough to be tested in detail. To date the aglycone has been obtained in minute traces as a dark tar. The only tests which it has been possible to carry out on this material are those using alkaloidal reagents. 'These tests have indicated the presence of alkaloids. The glycoside gives indecisive results when tested for nitrogen using the sodium fusion technique. Quantitative micro Kjeldahl determinations indicate p.7or. nitrogen. Assuming the presence or one nitrogen in the molecule and assuming that the glycoside Is pure, thi4 percentage of nitrogen would indicate. a molecular weight of 2x10".. Due to the irsolubility of the glycoside in the solvents usually employed, in the Rest method for molecular weight determinations, the molecular weight of the glycoside has not been determiped. The glycoside has been fractionally extracted using various polar solvents. The fractions obtained from these extractions all have essentially the same ultraviolet spectra. This would Indicate a fair degree of purity. The melting point is not sharp. As is characteristic of amorphous comppunds it softens over a wide range; melting between 120 and'130'0. Further attempts will be made to fractionate this glycoside fraction by countercurrent extraction and also by altering the chromatographic techniques employed to date. . Microenzymatic methods will be employed to determine if the glycosidic linkage is alpha or beta. 'aded to: 0� iortti- of: 18747 ham 1977 P '7 8 1977 Additional paper chromatographic tests will be employed, to determine whether or not the glyco portion of the glycoside is glucose or whetheF some other sugar is present. Attempts will be made to isolate Sufficiently large quantities of the aglycone in crystalline form so that in addition to the chemical tests, physiological tests may be carried out on it and the results compared with those obtained on the glycoside. qualitative and quantitative functional group determinations will be made on the aglycone if it can be obtained in pure form. In order that this project may be carried on more expeditiously I plan to hire one graduate student who can assist me in the ' work. Proposed budget for one year Equipment (including Extracto-matic, giant blendor, fraction /0oo,(00 collector, etc.) $,600700' Special chemiCals 150.00 Analyses and tests 300.00 Travel expense 250.00 Laboratory help and miscellaneous 200.00 One graduate assistant for one year '1800.00 Stipend for director 1600.00 . Total Respectfu 7 submitted Asso te Professor 0: b7 'writ : June 1 125eptenib0r 1931 =Pan; CL BY 187475 MIMORANDUM FOR THE MORE. SUBJECT: Contact - Dr.11111111 - . � - - 1. On this day the writer spent two hours with Drilla discussing various aspects of his project on the isolation of the active principle of Elves corymbosa. 2. The two bags of additional seed. were given to Dr. Be cnAicated that they would hold him for awhile, but that he could use at least five pounds more in the coming year. 3. The questioi of accounting for the grant was briefly discussed and Dr passed. along to us examples of the three-month ac mpli accountings which e routinely gets from the university. They are attached to the proposal discussed below. 4. Drillirsubmitted his proposal for the next year, which was discussed. in e ai1 with him. The minor addition was suggested to be added to the category of "equipment". Be has about WO left in his present grant, as of today. He indicated that, if November 1, 1955 is the starting date for the new grant, and if he receives the first portion of the money promptly by that date, things will go smoothly. -mormilim � . . 5. Dr.111111kaised some problems that he has in connection with having a graduate student. He wondered if there were security considera- tions in his having such an assistant. The Writer explained that there were none; that he should hire a person for the job strictly on the basis of his qualifications and his personal acceptability to/111111lb � 6. DrIllpalso discussed the possibility of another move in several years ram now, and 'wondered what our attitude would be about this. Be was advised not to worry about this until it became more imminent. 7. This bei the writer's first contact with Dr. 100W his impression of Dr 4 as very favorable, particularly along the lines of sincerity coud n egrity. S/DNEY /MB Chief TSS/Chemical Division SG: Dow:, o. b7- o : .18747 e Jun31 � E2 IUFDET; CL BY 187475 RECt;IFTS: ' March, 1954 Rec4pt A7231 7jlay A89I8 .Sopt.1 " "- A11593 Dtc. 13n':% A14396 FMEITP.ITURTLS: -Salaries- March 1954 3V498 - Dr. 11111011111111* . Cl..10578F -.Graduate assistant .JV634 - Dr. z.7752 x7911 - V1069 n Jvuo3 5v1165 071266 Dr. May July Aug. Sept. Ti Oct. Nov. Dec. Jan.-1955 - Feb. n Travel:- June 1954 niii653-DrA1164111110 .To meeting on alkaloid recelirch Payroll Expense- ' May 1954 n692 Weri=enls Copp. insurance Aug." JV956 ft - Vov. �J111206 " - ft Feb. 1955 ' . � Social security taxes Apparatus, Supplies and Expense- . March 1954 C1c9893F- Advertising in -Chemical & Engineerinfr Nees April 1954 Ck1005911 Triunion cups & other fitting Ck 10370F - Laboratory equipment Extraction Ck 10438F - ?lay 1954 Ck 1060k, - Heating mantel _and support Ck 10727C Cher:Ideal:7 Ck I065CC Internnl timer June 195h Ck 11457C 3 cartons Sowex Juno 1954 Ck 10380N lite exchange., becrbent ,i;r 1954 clt 11912N ctty cash- 44o Co 16 20 wilco co ./27 00 .e15 oo '400 oo -163 64 -163 64 163 64 ,163 64 -400 00 .163 64 4.163 64 74 �V 12 69 � 69 .- 982 8 96 '4( 10 00 27 89 V :1 925 co - 1 925 CO 1 725 co 1 925 00 2 64o 04 Ck 12043N - Deeminao ittpde ar. filter Ck 1227321 Labcraton Jack J71808 Funnels, rubber shesting, blade assembly, etc. Ck 12189N - Autemp heater and blades Aug. 1954 -.Ck. 11331:7 -4111000101WONOK Funnels, fr14' Ck 11353F - Dioxanx 1"" .77864- Ck Tabes, .mducers Be t Ck 11915T- � nt13 and support AP CI:. 1"/4 Phenen_azen Chamical Constitution Ck 12053- Ennis Invert:1.9e An.say.' -n 1 - Ck 121851N- � 0 1955 Ck .1..21911 EQUIPMLNTi Jan. 5 k 3)4143 121C8C-� - 1 Modol DX, roc .370 p , . #9 0 Less borne by Chemistry 1.)apt. (73) Total E.,�..endituree Balance Available in Fund at April 301 1955 7 475 00 5 150 00 967 6 6 174 76 6" 2.!.7 6 772 2; 907 77 MINORANDUM FOR: THE RECORD Addendum to Subproject 22. 1. The purpose of this addendum is to set forth the mutual administrative responsibilities of the contractor and sponsor. �-2. The.osibproject represents the research program of ;Dr. located at University, financed by MKULTRA, covering the period 28 January 1954 through 28 January 1956. .3. The 111.111.111111101.11111phas requested the University to submit to then a summary accounting of monies received from the Fund. Also, they requested the. return of any unexpended funds received under a grant from the Fund. 4. Title to any permanent equipment purchased by funds granted the University shall be retained by the University in lieu of higher overhead rates. 5. It was mutually agreed that documentation and accounting for travel expenses which are reimbunsablp by the University * 4h01 conform with the accepted practices of that Institution. ' 6. It was agreed that technical reports reflecting the progress of the research program shall be submitted at mutually acceptable intervals. SIDNEY OTTLIEB Chief TS4/Chemical Division 'AHROVED: .Chief, Technical Services Staff Distribution: Orig & 1 - TSS/CD 28 Jhnuary 1935 - MEMORANDUM FM: THE RECCRD : Extension of Time for Project NKULTRL, Subproject 22 Since Subproject 22 has expired and there are still funds remaining in the project, Subproject 22 is hereby , . extended for one year tron date of expiration. FROM: SUBJECT: Please return MEMORANDUM RECEIPT ' _ /310or oits�Sa CHECK11iii,v08 IN THE AMOUNT OF pig .73 RECEIVED. AUG 1 2 195.4 � , signed copy(les) of this receipt To FORM NO. � 36.66 MAR 1849 TO: FROM: SUBJECT: MEMORANDUM RECEIPT hereby acknowledge receipt of the following: GHECKSriN THE AMOUNT OF .15.07"?-1,A3 � RECEIVED. Please return Rgnod copyiles) of this receipt To F0nm N0. 3 .68 MAR 1149 SIGNATURE OF RECIPIENT GATE RECEIvE0 G 25 1954 URE CIPI NT . � ��-��-�-��'... .....��,,�mtr.... .........��,......,��4 "�� 5V- 1. Imola. No. 2 for Subproject 22 of Projeet HICZWIA Is attached. This invoice is the final one to be submitted under aibproject 22 and when paid* constitutes complete paymeut of this project. It Is requested that payment be made by two cashier's checks for $20.33 � sod 0,133.33 mode out to the be check* should be sent to 011ie, TM CD through TSS/Budget Officer I-by 16 August 1554. ., . ..� 2. Tin WO Invoices covering this Subproject aggregate 08008.03 and represent the total sapendlture to be made under this Subproject. It le requested, therefore* that the files on this Subproject be *Used. � I CERTIFY THAT FL2-*""`' ,..VAILABLE: OBLIGATION REFF.F.CIIC: dolegt r MARGE TO ALL:Ma:IT No, flik;ei 2 �10-0.# UG 6 ENT� AUTHORIZING OFFICER , 43/4�7 oast_er CH EC K#4342611 IN THE ANICIUN:11 $423.1.40, RECEIVED.AUG 1 2 1954 (2) It is hereby 'certified that thls inVOLOS, applies to Itubprmjeot 22 wider Projeat =URA vtdob vas duly approved and tbat the project, has been carried out In aceordenom vith tbe DWP memorandum to WI dated 3 Area 1933 NA tbe Dn watorandum to DOA dated 13 April 1953. This ia to certify that I have today received -Csshier's check No. 24I38335 of theVall,a~, Oft in the amount of $102.67. , This is to certify that I have today received Cashier's check No. 14138334 of theallatiiiiiillakoe, ic:\ in the amount of $2,566.67. -* MEMORANDUM RECEIPT I hereby acknowledge receipt of the following: CHECK -1kM3Itlifq THE AMOUNT OF RECEIVED. maT oar= Wet Choodeat Division" TSS taarimentos Invoice CaratIcattats 1004.4i FEB 1. S ENID FEB 18 Drill (1)' t Is hereby Certified' tb4idae is TSVoice 204� 1 applying to' - t 22 of Project leCUISIA0 that technics formance by. � � teat astisfeetem that the worh is being ececapor limbed occordergas with the actual egreelleat noshed lath ot that this hill is just opt correct sal that persixatZberear has rat rat been =dew (2) *It is hwrehr certified. that this invoice males to leespreject 22 =dew Project MILO& ebich as day approval mg that the project � is beteg carried out In sceordsace vith the 1:40P reacraulea to =1 date 3 April 1953* the DOI ausureadua to WA, dated 13 Apra 1953, and the ret seacrsalus to the DIVA dated 19 June 1953. , I CERTIFY THAT F AM: :\VA'.LABLE: 0040ATI0 :" *ma& To ALI. S.T! � IIDDSMANDIDI FOB2 ..COMPTROLI14.. . Pinence DiViatt� on. " SIDLIZOTt�!reject 10031.12ki,:. stsbirOjeet 22 ex the authakty ariatteiIA the seams:aura dated. 2,3 April - .,�� - _ _ 1953 from the Da/ to the DD/A, end the further authority granted. �� , a_ to the coop. In the memorandum dated 17 April 1953 frm. the SID/A tro17.er on the sub,ieas IN)/P-TSS Project NICULTPA,.� and. tbie me- . .,..� :*._ '� - � � � � - *morendum date. 19 Jinn Trey& the DC1 to the DD/A extending , 7- v...-;44:�?.. � � . � ..�/� the authority to cover Fiscal Seer 195k) Subpro,ject 22 has been eppr.ovedo end: 48)046;00 of the over-ell" Project )1113LTRA.- funds have been obi/gated' to cower. the 'subproject's eavenees. OOTTEISE Chief Chemical Division/ TSS APPROVED FOR OBLDIATION OF FUNDS; DIstributior: Addressee - Or1g4 A, 2 Bzgm. Seey02 Bee. Dd. - 1 TSSAudget - 1 IZS/Reppilstry 1 TS:4CD - 2 IMPDET CL BY 187475 MEMORANDUM FOR TES RECORD. ; . 1. Saproject 22 is a continuation of Subproject 1 which - had as its scope preliminary work on the isolation and oharacteri- zation of the alkaloids of lmoomea Sidafolia Choisy (Rivea corym bosa) In order to determine their activities as agents affecting the central nervous system. � 2. The results of the preliminary study are such that a-full �roaram of research on this material is desirable. Dr./(11110111111111111 � who conducted the preliminary work, has submitted the attached .r.posal to carry out this work as outlined. by CD/TSE. 3. The .;;'; will serve 8 as cut-out for %,rans er,of fun $ o . The estimated cost of the work accordin to the proposal is $7 .Q. .O fo ne year. To this must be added L6 service charge for thz mount- ing to $308.00. The total cost of this investiga on or a e Oi Of one year, therefore, will not exceed $8,008.00. :� 4. Dr has been granted a Top Secret Agency clearance and a. is fully css.ble of protecting the Government's interest in this pro- ' ject. _ Chemic Division, TSS APPROVED: r4 u- 0 C ief, C.emical Division, T= � ei ...I a. � PROGRA1',I APPROVED APPROVED FOR OBLIGATION � AED RECOlv.1.1EMED: OF FUNDS: 1-.1 '1 C3 , . re n 1--, ',4 c+ ;-- 1 , C0 0 ',4 0 ' .. � ciy., Res. ad. Attachment - Propoaca .Res tor Date: tif,134. Anuary 4, 1954 , :Dear Sir: I am aware of the interest your Foundation has in , supporting research related to physiologically active com- pounds. Recently I became interested in determining what is the active component(s) of the drag "Piule". My vork together with the meager technical literature available on the subject convinces me that, financial support will be necessary before I will be able to continue the investi- gation., I am, therefore, submitting to you a summary of the work completed on the problem to date, together with an estimate of the funds that will be needed to purchase equipment and supplies and to employ help. - ' University officials are aware of this request. If you see fit to suppOrt this research, I plan to employ.. .one or. two graduate students who will work part time on the problem while working toward the M.S. or Ph.D. degree. My work i the 'field Of" planb-aikaloids'dates back ,to 194 ' Most of this work was carried out at the University of When I moVed: to University in the fall of 53, found that no work is field had been 'carried out here. As a result, very .little of the necessary equipment is available. Departmental funds are available for some, but not all, of the necessary equipment and 'supplies. The funds requested for the first year of the investigation, therefore, include these. Assoc ro essor 'WARNING NOTICE SENSITIVE INTELLIGENCE CES AND METHODS INVOLVED .1+ � ummary Of Work-On;',F,Plulw The seed of a trailing vine Which grows in Mexico and Central America has been used by natives of those regions since Ancient times as a narcotic. Although the plant has been classified by taltonomists of the ftithsonian institute - . as Impomoea sidaefoIii Choisyl a plant of the Convolvulaceae family, its seeds, because of their physiological activity, have been considered as one of the peyotes". The most common example of a "peyote" drug is the button from the cactus, AnOW.onium lewinii. Until the work reported here, the only work carried out to determine the active constituent(s) of these seeds Was that by C. G. Santesson of the Medico-Surgical Institute in Stockholm and reported in the Archiv der Pharmozie 275, 532-537, (1937). His experiments, which were conducted on small quantities of seed, Indicate that a water extract of the crushed seed contained i glyco-alkaloid which had nar- cotic effect on frogs, mice. and rabbits before and after. acidic hydrolysis. The presence of sugars was determined by WaRYIS of the Fehlings test. The evidence for reporting Alkaloids as precipitates with reagents such as'phbsphomelybdic and phosphotungstic acids. In the present investigation, which was carrted oat during a five week period at the mall gaRntities (15-60 g.) of the seed were uoed to NN MICE repeat the work of Santesson and deterMine the best means o extracting the active .constituent(s). r � -- sample of the seed, crushed to pass a -.Finally a 100 g. 15 mesh sieve, was extracted in i sohxlet with petroleum ether to remove oils. , The de-oiled seed was maXcerated With distilled water and the mixture centrifUged to separate the mare. This mare apparently was devoid of alkaloid or glycosides extractable with various polar and non-polar organic solvents. The �- � . aqueous extract was freed from most protein material by ' the use of trichloroacetic acid. The deproteinated extract was neutralized by means of ion exchange resins. The effluent' from the resin column was concentrated in vacuo � � , � � . � . resulting in the precipitation of more gummy material. The supernatant was clear, viscous, dark green and intensely bitter. It contained. no free reducing sugars. Tests with alkaloidal reagents were inconclusive. Attempts to extract alkaloidal material from this solution using various solvents were unsuccessful. Emulsions interfered with the extraction In b;Ix neutral and basic solution. The aqueous solution was stirred with methanol, a procedure which removed most of the polysaccharides, which were present in large quantities, and decreased the viscosity of the aqueous solution. The resulting aqueous-alcoholic solution was oonct;ntrated to remove methanol. It gave positive tests with all common alkaLoidal reagonts and negative tests for reducing sugars. 'illARNING NOTICE t..":�;�",4'4 ' minute quantity of crystalline material formed in �- this aqueous solution after a day. This material has j�i�;- ;4' - . -not been identified. � The remainder of the solution � � was evaporated on a water bath, in a nitrogen atmosphere, ";� .� ;IP* � � Without obtaining �additional crystalline material. The residue was taken up in water and more polysaccharides precipitated with the addition of methanol. Repeating this procedure eventually removed all of the poly- " . " saccharides. From ihs�supernitant, after removal of , polysaccharides, no crystalline material was obtained. Tests on this solution indicated the presence of alkaloids and the absence of reducing sugars. This � 4 � solution also was:intensely bitter, a crude indication of, the presence 81' the active. principli(s). of "Piule". No test animals were available at the time for physiolog- ical tests "of various fractions. This solution was hydrolyzed with 2N H2804. 1). voluminous amount of pre- cipitate resulted. The clear supernatant yielded excellent tests for alkaloids and reducing sugars. Paper chromatography was employed to confirm the presence of reducing sugars. The polYsaccharides were hydrolyzed and the resulting solution, after neutral- izat:ton, was subjected to paper chromatography. The results mplete; however glucose, man nose and cylose appear to be the predominate monosacoharides present. RNING NOTICE � .101.00.0.0.1 sit � - At this point Work was suspended due to 'potations. Proposal Future plans call for the following. . . � . " change of The use of larger quantities of the seed. (Originally only a small quantity was available, now there is a large quantity available.) 2. Considerable work, on the use of ion exchange resins in order that this technique can be used on aqueous extracts of piule without the possibility of losing some of the active principles. Work to be continued on the isolation of glyco- a/kaloid(s). There is some indication in this investigation that in. addition to one or more ' glycoalkaloids there is also uncombined alkaloid. 4. Work to be continued on the identification of the sugars combined in glycoalkaloid moieties. � 5. Test animals ta be.used to determine the presence of the active principle(s) in various fractions. 6. When the glycoalkaloid(s) or/and alkaloid(s) have been isolated work will be undertaken to determine their physiological activity on laboratory test animals, their physical and chemical properties including ultraviolet and infrared spectra. If the alkaloid(s) cannot be identified as known alkaloid(s),, work will be instituted to elucidate the structure. It is estimated that this investigation will require from three to five years to complete. � :ow wA I NOTICE 11V.v.A .Funds Needed for One Year .Equipment (includes special equipment such as BeckMan �. . . ,. ADU spectrophotometer, centrifuge, sohxlet. , ,. extractor, paper chromatography equipment, :-,Kofler hot stage and small mill for .. grinding seed.) $2700 Materials (chemicals, solVents) .... OOOOO ,........ 300 Salary (senior investigator) .� � . O O .1600 Salary (graduate assistant, 11 ,Laboratory help 100 :i;IENSITIVE INTELLIGENCE at the vedfatit MVP.= Project =TRW ea the se- date& 1.9 lines Inti !Vein the =I to the WA exteollne -�74(tho'elty to eons; Pleeei 'Tear 195� albprojeet itit has 140116 enroved unit 4801308.o0 or the ever-ell Project ItCOLS4 foods be beat cialseittet to *ow the eshprojeeVe 031plaies� SOW COM= Chtet Ohm lost Diviatoni MEMO YQR OSZIALTION 07 1111125s I CERTIFY THAT � ,sturo. *ft. -ORIZINC; OFFICER � �� Inform MEMORANDUM FOR: Chi CD/tSS SUBJECT �� 4 frP� � 41 SO 2.1266 14 September 1953 Reference is made to our memorandum dated 29 May.19531 requesting a covert security Clearance to permit TSS to utilize the Subject's personal knowledge in connection with a highly sensitive research problem incident to Project MKULTRA. . The Subject ' will have access to TOP' SECRET Information. In'accordance.with'the provisions set forth in the Directorts -Memorandum of 14 February 1949r entitled �Security and Operational Clearances", a covert security clearance is granted for the covert use of the Subject as described in your request as set forth in Paragraph 1, above. . � , 3, The Subject is not to represent himself .as, no is he to be represented as, an employee of pu., . . .., , 4. Tour attention is called to the fact that a covert security .clearance does not constitute Complete compliance with the provisions' � of CIA Regulation 1111i Therefore, if you should desire at .a later .- date to change the status or use of this individual, a request for clearance to cover any proposed change should be subnitted to this office. . .- 5. This clearance becomes invalid in the event the Subject's' services are not utilised within 60 days of the date of this , memorandumn. Ar a son & Security Off er DD/P/tSS /dlb (14 September 1953) � , 4; Distribution: ' Addressee - Orig. (Sgott1eibb,100111111.140111110111Millir 1260,- 2 � � Security Information

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MKULTRA SUBPROJECT 22 (109 DOCUMENTS WITH PUBDATES FROM 530914 TO 63050 1)