STUDIES OF RHIZOBIUM (NITROGEN FIXING BACTERIA)

• Document Type: CREST
• Collection: General CIA Records
• Document Number (FOIA) /ESDN (CREST): CIA-RDP80T00246A021200150001-9
• Release Decision: RIPPUB
• Original Classification: C
• Document Page Count: 54
• Document Creation Date: December 27, 2016
• Document Release Date: February 26, 2013
• Sequence Number: 1
• Publication Date: April 8, 1963
• Content Type: REPORT

Declassified in Part - Sanitized Copy Approved for Release 2013/02/26: CIA-RDP80T00246AO21200150001-9
This material contains information affecting the`rvoti-. 1 ,Defense of the United States within the meaning of the Espionage Lows, Title
18, U.S.C. Secs. 793 and 794, the transmission or revelation of which" in ony.k,,.,anner to an unauthorized person is prohibited bi' low.
COUNTRY
DATE OF
INFO.
PLACE &
DATE ACQ.
THIS Is UNEVALUATED INFORMATION
annual report of a Polish research
REPORT
DATE DISTR.
NO. PAGES
RFFFRFNCFS
Studies of Rhizobium (Nitrogen Fixing
Bacteria)
project entitled Studies on variability and Genetics of Rhizobium"
being conducted at the M Curie - Sklodowska University locatid in
Lublin. Jadwiga Ziemiecka and Zbigniew Lorkiewicz are the principal
investigatora of this research. The period covered is 1 Jan-31 Dec 62.
OFFICIAL USE ONLY7
GROUP 1
Excluded from automatic
C 0 N F I D E N T I AL downgrading and
declassIflcation
ARMY
NAVY
AIR FBI AEC
8 Apr 63
LI
ki -~~~rp -RT INFORMATION REPORT
NO DISSEM ABROAD
CONTROLLED
DISSEM: The dissemination of this document is limited to civilian employees and active duty military personnel within the intelligence components les
controlled
mem
informa in
officials
r
of the accordlanceewih p~agrapha8dof DCIthose 1/7'it may be of obth seecomponentsofithetdepartments andtagenion. of the nUsSSGovern ent directly . It :,aiticipating in the production of National Intelligence. IT SHALL NOT h BEto DI heE UISAGovernm nt witho CT tORS he writs disse
thenonginator. iza-
Declassified in Part - Sanitized Copy Approved for Release 2013/02/26: CIA-RDP80T00246AO21200150001-9
 Declassified in Part - Sanitized Copy Approved for Release 2013/02/26 :
CIA-RDP80T00246A021200150001-9
Declassified in Part - Sanitized Copy Approved for Release 2013/02/26 :
CIA-RDP80T00246A021200150001-9
 Declassified in Part - Sanitized Copy Approved for Release 2013/02/26: CIA-RDP80T00246AO21200150001-9
V 11
0 Dt partme ,l. of Geneie, robi,oi..rrfr
I+oie],t~d~~ra ii~rere.~
0~ Ago R ~jawickLs Lublin Poland.
2. Mrs. Jadwiga Ziemi eka and Zbigniew Lorkiewiez
dice 't Variebil._itY and >:afai~~biun~
50X1 -HUM
f tKY
bF, tIT1,1101"L USE
Aeriatrading ana
L Declassified in Part - Sanitized Copy Approved for Release 2013/02/26: CIA-RDP80T00246AO21200150001-9
 Declassified in Part - Sanitized Copy Approved for Release 2013/02/26: CIA-RDP80T00246A021200150001-9
8e .ary
Our g ne.ra __ purpose i to carry
tea.
fundamental studiee s on the
genetics ax,r1 variability of izob iumg which in, these batter a
is exoep t..onally high. On the basis of our r ~ su ~ 3s we shall Pry
to obtain by differen= m?e~. 1, strain e of Rhizob tu.m with a high
nitrogen fixi ig power,
have started, our experiments on Oo aber I 1960,,
From 1O,,11960 to 12.3`M962 we have performed c~mpl e irm sti
gat Io ~:1,h cs~ cg=, rshyig ar, a gene
tical, properties of 'about: '150 strains o
Beal-,e.e s that,we have co:1.l eote t phage str ..i'L.Ys and isolated
b ac rk'v r? a l and h age mutt .t a ti
Cytoi4. gioal studies.
-w.,:.e.d,.,..,- ---a i. >t i at3_oi of Slow
growing strains in. their cultu eO ar not suz tab1F: for
Pr. e ar ~.rg
the pbaxatr inoe-ularits!'!
'of
The alt ..n product' by differe.n strains of Rhizob::.~ was
reported by marl.,r, aazttz Y?: b 4 M a o x)~` /
f for d17,71-t G e r g I and B e g u I n /B T i-.
in a a /6/ enAPh:ha8ize the. role of aux:.r_ in nodule. fo
and growth. As the pre-se no . of bet~a~in~ l.ea yeti . acid i~;
Rhhiz `bj um -- S. infiuent:f; the effect of thir:. 1111.croor a2= II !) .
leguminous planfts oa.r i 1v'estigatione were carried with
resF~~ ct aux'.izn for .a-t i.o.a. in differ; ntly treated c ltu'res
of i .i7 obium , Fallow?i+ 5 ? trai ns o ?` _ obi~3.m t 3 et aIna ?f
yo a~rifo] ii strains of Rh.me1:1lotj end 3 str'aa. r of
Via.. japonicum were grown .in liquid media coxntai:rairt; vaxious
nitrogen coal oundsa y9a6t extract, .Ins hydralysate
mixt.-mr-e. of aminoaoids s ' g1,,jrcine a:l ar~i. : : a i.n 1e1~6i..na
prol'iiae, phenyloala-mine. , aspact.-L acid. gluta tic acid tyrosine
and cysteix.e, tnyptophar.~e or seri..rie tie "j.. zg added separatel7
Bets.-indolea.ceti4s acid /I ACA< / in ul.,ures
was detected by
means of paper 01..romatograpby, to V a n u r a
and SM a c u r a's /9/ modification. /",'.)60"/.
The spots were identi.f.e . by comparingthem with Stan-darts Z&
with various concentr i.t .one of l,,ArA iC-t wa.f3 ~str ergoo p that
the presence. of tryptophane was necessary for, IA- , ~ovvmca ioue 4
Declassified in Part - Sanitized Copy Approved for Release 2013/02/26: CIA-RDP80T00246AO21200150001-9
 Declassified in Part - Sanitized Copy Approved for Release 2013/02/26: CIA-RDP80T00246AO21200150001-9
eta-rinc o eacet .fin acid wa ~ t c e I'.,' t 73~.~ 1r ? ~ ~~41,'
(u:ltux~e of two effectir strains )f Ph-tr~zf bu- also
J-11 the u1tu; of one. pa ^asjti s strair, -h--OM- among 3 st m Ors
of .Rhomljic)t, only I strain gave, on .
-ma. crams '~A d
iia t.r spot R I.A.A. JIttij,
of of RIG
y :3 i a1.! tea;
did not convert t..yptophans to I0AoA0 :.no did not r t1 .
this, a x ?~ in ~u experimeenta.2_ CQnditi_or? n
f ie o f ':Lizobium cultures nl&. e a c-ns5.dera1- ti r6"
T ho a prod u .l . 2a It ~ a nct
t hat for tion
beta- info: eacetid. acid p. oduction 10 f,o 5 d `- old
cu.'-buMs of . i z >'bium we r(, most suitabj