FOOT AND MOUTH DISEASE

• Document Type: CREST
• Collection: General CIA Records
• Document Number (FOIA) /ESDN (CREST): CIA-RDP82-00039R000100170030-0
• Release Decision: RIPPUB
• Original Classification: R
• Document Page Count: 154
• Document Creation Date: December 22, 2016
• Document Release Date: March 22, 2012
• Sequence Number: 30
• Publication Date: January 11, 1952
• Content Type: REPORT

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STAT
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Foc1I AND NOUT { L I51
LGrtinrcI
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o1' kh i?
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INThOWflOH?
F `ty Q4 r o i o n 1?W) Loeb tor, oche ? c h1enhuth
o tab d the 1ter b e nature' a the ihoit nt o1 boat-ind-
mouth di a oo~ Thin bed b in preooded by tlw r n rkob1o dieoovery
oS the Ru ei reoo rohor A, 1, winovsidy,' who, studying mot c
diooe a' tobdcoa, ?rt otablohdd that coeih inoitto of
dieoaeg in plants pa~o through botoki~i iltorc.
oientiiic data in the dthmgh of thv study of ?oot.~nd-
mouth diseaoe, after it waa established that the incitant of this
inliotion is of ?i1torab1e nture, wao onrichod by whole sorioe
of discoveries of rat thcoreticai fled pr~cti1 sign ficr noe.
it surfices to recail such &r outstanding di~cov?ry of tho rench
it vesti ators V11 and Cara i that of the u1~r'a1ity
rnn stv~enna of yps of the foot.nd-mouth virus . Th9se
inveoti abard' discovered two immur oio is iiy d tTrer t typos of
tho ?oot sld-heath virtc, Indic od oa on 3 Ly by them with
the letters IIQtt and U J IP
This was not only confirmcd by nib equont research, but
~iv~n ndpp1entonted by the discavory of ti new type "C" ? In
addition, there woro revealed numerous variants which could not
be aseoojabed with any at' tl~e indicated types of the ?oot.nd-
mouth virus
At present it is considered that the estence has been
proved of three imm?ttno1ogica11y different types of t e 'aot and-
mouth virus
types.
"0",
r,Ait, and tt~tt, end of numerous variantz of these
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T~ new. prab1am Qua
ht. T ~ baQampatifl
1rfl t ~arn~whii1t rant ii~
.~ati'ia b~aProPhylaatida ~amawh~t
pr~cttca to ~a1va prablam~
dUrantLys ObvlauslY, y vuact bi apprapnratiarca au1ct
fl th~ bItt1a Tat
rV mote a faat va 'aau1t
1iiee than a A c n a V1? ? This is 5a- tli
prasant era in tha solutiar a~ tha prab1am a m~thaU a
~qairt~Tt '?ao~~fltImQ~th dir~a~~e~
aativa nr4 ptv@
Ti pr toblom of rnotho~t~ af Y~ccin~~ion
h
c1U i' Y tO1V d ~t the pr8 nt momAft.
may be cons~4sxed to ba con
in oral : n countries el~minu~l-hydroido vccine,
~ nvmb~ar a~ ~ ~
being,
e~ic~~d in ~Q~n~y~ ~~
c ~ ~
;~rel~as?d by Dar~ish in~-ncti~taZ nd
-tie ctrug~F1e at~n~t ~aatd~mo~a~~h dicease.
apped success~ly i~
Qreat 1i~ht hao now boon shad or the prohlom of vir~s
~ ~-and~rnout~ :
~ o
carr~irg in concoctio with diaease, which is a
,,.
yen laygicn1 ?e~ctor. it it be considored to be
Y serio~~s ai~o~,
proved ~condition~y that ~ certain portion ai' anitnn1s which
'
~'oot~~nd-ntauth disuse romein viarus~cartn~
have coetrckcted the
from his is derived the n~aces~ity ?~
over a long period
me.sures with roSpct to enirm-ls which
?
hsve con t.ndfLouth disuse. In many countries the
~rr~c~pd boo
ryes which have been adopted with respect
periods cci' such q~,eranti
to have cnntrocted ~cot~and?mouth di.set~ee remain
~ls which h~
withott adequate scigntLtic foundation. Practical precautions
beer a cert~in contrast to cjentific data with respect to virus"
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c i s n QfY Ut Of With ~ ? EQ 4? T t
eo ontt 1 to 'oont nuQ with ~t U rQ tQr ur enoy tho twdy oL'
thin rob1wr doaur~lato ?rth r 4ata .i on p tici1 p ri"
moni tion n ordor to form final eonclu ano On tho o oc '
1o icct t#eli+iF/~knQ l of vitu -c r +f M. r6t4kt1liiit qu ralttinlFt
'with ro pect to w tn~L 1 which have 0Qntr ctot 'QOt n ?t QUth
dt;Q~sQ
Monet hat 1o brought mtzth that is new to bear on
tho qu stionc oi' ti atubility off' tho foot'4nd~mouth viruc in
an oxternal medium, which hap substantially broadoned our oon-
capt~ nth respect to the epi oolo ~y of i oat-and-mouth diseaao.
Interesting work on t thi question has boen Barrio t out by cur
Soviet invantiutors.
Coritempoi'ry investitatars navy us yet dorm vory little
the area oi', r searc1i on new and perI otion of old' mctnodn of
in
foot~and-mouth disease thorapy. This is Qxplaincd by a certain
underostimation of this extremely important problom, whose
successful solution would help prevent numerous corrplicatians of
the basic foot-acid-t~aUth disease, and would help to decrease
/oases from this a'fliction.
The study of the epiodloy of foot-and-month disease
nos been compietaly inaoequata. This his happened becauce ninny
questions of this affliction gave been decided in the laboratories
institutes and experimental sttion, and not under aonditioric
of
of the epioo ogical medium itself. In this connection practical
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thv )u~nQV o~
= o v n irk kjig intO
o1
QQ vvvtorv on t
mou O t
torU houW b bo 1ht in too pr
a~v~ti ob~vvv
. Marty qu at o th off' tv f oot-
. ooo~a~~,~~b bit=~v~~
t1 of v( only t A b ~t.:V~
o~~ ~ bt .
rtavt v e
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Fr L tM .
Ot N M
qtr
is mu'. nP,or d in w Y ooi tr o~
~oo~~~n~~~m~~~-~t ds~
o ar Qp q 1 i rt ,
riC13 M;ta ciitk~~~ op t zooio0 0,t!
:n t wbaio 00
mo?t- doorsaoi d+ing hot 10 or
b no
r :n t ?o th ~ttm 1 Y*ii, , vid@n i
~ rtat rotn 1925 to 193
~`~
from data co11ected b'1~cii or for ?th o
. 1c . Ia1y, iu o 1avi j aoao, Sa~tn, R. rams,
~~.~
and . to11w~$i
1oi1 widG :19 th~ prod of ?oat-4mci..mou h di ~ t mA r alt
4 4.t ,g.e be to ~Gkl o the
be soen from the Laot thai in 1935, aorordnt
B1troaU or I ndu i of he U (oh1er ), 66
st ands .
rie3 vrora decla~r rctd with rrpc3Cb to
diseases
d~ta on the trGnd a ?0nd^
~olovt-ar? adducod certain
disease in individual Countries
mo~th
=most diese epjZootic in the
U. Ou,tbreaks of ~ooand
.
Us have beri ed in the ears 1870, 1880, 184, 1902,
1914, 1924, 1925, and 1929
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'rho outbreak ofoothdmouth diooao n 19Od woo tho
most ub tintia1 o all epi oot o in the U L'or trio previous
yo'o
in 1i idatinL the ep ootia more than 1OJOOO head o
e ttlo w rd 11ad, at a cost. of hot o 11iot da1lara,
~ ~k1n. A aohsido2 b1o outbreak o footed-mouth
dit oaso wu i noted in En ttind as o '1y a 1t39, aftor which this.
sitesc did not appoar for 2 or 3 yonro. Froth 1877 to 184.
the Foot- d~mouth dioeauo w~c notod a ua11y, t1~e nwnbor o
in!:o tod ?artr tondo at timos ranching 1,732 a yoar, &nd tho
htmbor 'ot i 'octer cattlo iOO,000 hoed` (Hwtyra nd Marok) ,
Irt tho period from 1892 to 1912, in spito of the com-
pulory kiUin of stock on h11 afflicted fartnstoads, 15 morn
outbroaks woro rocordori.
Strong outbreraks in &ig1and ware oosorvad in 1922, 1923,
arid 1924. Tho opizootic of foot-and-mouth dice&tse in 1922-1924,
which brake out .first on the eastern coast off` England and sub.
seq ently spr u1 to the south and to the north _of the country,
was the tnoyt se1LOU epi2ootic for the preceding 30 yoars (Zwick).
tXrin this epizootic 1,140 soparate outbreaks o foot?ad-mouth
&Lsetaee were recardw1 and in this connection 53,599 heed of cattle
were compulsorily Id11ed.
In 1923, 1854 outbreaks of foot-and-mouth disease' wore
rocorded and 125,098 head of stock were put to death; in 1924,
of Loot-and-mouth disease and 88,726 head of
1,440 oubroake
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tQQk ff i ere killed ( or1e m Commli~lan on the tidy o F?ot'Md
~
Mouth Dl); in 192, 204 tbV6a1e aaI iY% 1927, 19 autbrea1e s
able moi'ta1ity ?rom Vootaad mvu h dl oa sae
In 1922 Qonmlder
~. up to 2.~ ~ pera one on the aver z e n 1923 thU
oboe~v
martallt~ w itL11 hi~her ; mn4e up to 3.1 moat
the firof 1n2'ga outbrodk o oob u mouth dl oaae
n ?ranoe Qd~nilyr oacuQd in 193, after whloh theeaoo
I
wt ob: v?dI annually.
n 1921 ?ootd~mouth dlceaoe a~Qrtod, in 53 reparti~ento,
poplatiof points with 2~3 iar aocted by tie dteeaoe.
6
from 1925 to 1933, an avorae' oL 134,175
In cub~~~~~nt yaks,
o noted annually (FluckLgor).
new CMdi the ~C1~n
Thy:re way a lao outbreak of ?oot afd-IAgttth dUe ui o pi."
71ootla in prance in 1937-1931, the die4ase havlnC boon brou~ht via
..
Mareelllee from North Jfrlca, together' v ith imporber3 vo etab1GU
and hoga. Almost the entire country -- 72 oC 89 departments .~.
c oohed by the epiootiC in a very short period.
t.andmo~rth tease in Spain is evldenly
?-~'oo
static In For the perio4 from 1925 to 1933, acoordln~
c~aracter.'
to fl1cjgars data, an average of 14,519 new rtLections were
.~c
recorded annually.
- nmari~. In he periodrom 19o4 to 1912 Denmark o free
of faottnd-mouth disoase CHutyra and Mari" .~
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In 1912 f ort.tn4-forth w~ br ht nio 1 n r
and ao uirod a~n idorablo oproad tho'o - I~O13 rin word
b it? roa d 1919 the , aot ri t L
o ,od y
ta~
a~
:rn 19 i'aotpant-mouth ctieo ao wao t to wn it into t n rk,
in Lt11 ptababi:u by from rar }t, arv r~ w t th ro n th x opt on 1
rd dit it inrect1rcnt of all pert,
Tc w&u'rt the b~ tnnin of 192, , 0 O oaf ~ wort rncordod f
uboe ~orrtl th 'o ban door? aoo ern thb rtmb r oL' farms
atfectoc1 by foot"and-rra~tth ctisoaso, but in 192 thtwo bean
nrw incroaoo in tha ooioobic viravo. This tiara tote oicoec
sproad to Jutland, whore in ono month tt1ono (May) 3,OOQ net
wex'c Taco :cdf Inciaonta11y, a pt 50 pe orTb of nil
case
ar rn is which had become i 'oot4ct dov&opod Boob-end"mOUt
diaeaso agsin the following year . This i evidont oxiair~od
b the ci.rference itt typos of the vino which cattsos vnriaua
epizootic
}a ~lM. n Otwrnuni footAanct- IOUth discrase is observod
contirnuot.1sly as a state c sickness. Epi~ootics had already begun
to be r osltGd from yoar to ye~rr in l6. , The strongest outbroaks
in GeV y wore in 1~IJ8-1392, 1 994901, 1910-1912, 1919~l92l, t t
Duving the 'oo - nd~ ont dis aee Opp.??otio of 1392,
193'7.'.1938.
rrl.fion heLtd of stock developed tho dice (Zwick)
After a brief interruption, in 1896-197, a lure outbreak
occurred again' durin which about 3 million hea f stock became
infected. FOUavrita, a rather protracted period of na&lmoccurrod,
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but ir4 191 49f a new WQ.YQ
h1 o ~A2 927,6?4 a,
iw' ttokad by a?t?r~aa~~t d~aia a
In erint o~ 1914 n rw jas ra&th tn the d~Va~pPmaflt a1
th UaC opicOti b~ . tWa TdWaZd the ~flt
o .U1a~~With i/ ,964 t'arm~ a
this yaa~ it abtaak7 ,O/~a ~ p~
irt 19119 h Ei ' v rO1` upt r~laoti? 4!ro1 out Vltktt
niti aanno~ bii aornpara~ ~rtth and faot~
itt propoiand and: a~a
an-mout~~ iMa~ Q~~i?100
in ~ ~a tho faa b that in `rho ?~U at
Oorma~~~~ It ritt~~~aaa to pa
1920 bha dtaaaao atekad ~,a papa$an poinb~ vv1th 1,Ob7
in'jct j`cr~n.
atla
~~~ / m~.1,l~.01 head: of c~~
~~~ ~~
t)~ng the aori~ i91 1
j~oaso (2 porn oI X11 stock
were a~~ac~~od by
In 1926 in UarmtnY, 1S000
a~nad. at that tima in Ormany)
dwma~th d i e a~Q wa~a in 1927,
?~rmo jnfactad ty food,-~n
15000 farmt, in 1928, 23,00 and ir~ 1929, L,000 t`arma
s
(Wa1dmann) , F or the period fram 1925 to 193 avora~o a5
/k 090 jnfactod points Way noted annULt11Y (F11?').
r~~k a~ fao~~nc~~?mc~:th ~.s~aasn
In x.937 a sub~?baritia1 ov~t
b
occ ct~;ng a ~,ar~o area of the country`.
urred a~a~n in C~arm~`-Y~ a ~~~`.o
~afl1pmouth di~aaa~ hart boon braht in
In this inEtaflc? the boat
fr~am Fanoa
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~ e rn Hg'y oot.mn ut ~ ~ o into aAnp
in 192o?~19a nub of o irtot u o a of a n~i ndnt wave of
ootcndpmot~- dioqtg~oa ?wore nctod1 aooom&4od by masoiv? Qtocka
morta.lt , In ono taro herd tho mortaiil y among cht1t catt1c
r aoh d 0 po 'oont
Ui i 1&. In Rw a `oo -nnd~maut disc oo i~ off' ethic
chharactor. . For the period from 192 to 1933 nn avora c o1
3,56 n?w CLtsOs oi' o~m~hofW oL thv diso u e word rsaocrdcd
onnttaUy (Fltc or) .
J1i w , In prQrevo1Utioft-t7 uosia 'ooit -nc mouth disoase
w s otatic ~tcknoss ? In t3 enco, no stru cle with this Widtly
a
s read scknesd was carded on thon. Thy rst dtatistica1 data
on the s redd or root~nd-mou h disaase in Russia arc ?cund in
tho annual repots o the Veterinary Administration o the
Mini s tvy of n er mal Atr'ai c . (data on the spread or foot. nd-
mnci1h disoase 'Lor the p riod ?rr m 1 81 to 1912 have boon compiled
tho bases of roporbs of the Voterinary Admninistratiort of the
on
tLnistrY oJ` Internal Affairs, published annually in the mLainn
I
rl~h,v Vtdrinarn 3& We submit theue data in two table.
In individwal gubornias, stock mortality substantially
eccasded the avera~o delta subnm3.tted in Tablo Z
-10-
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TA5LE 1
'
mb6P
Mortalitioo
Oubhidc
Number ' o
Ao t P roent of tho`
~~ESf
and oblatto Attacked
by Foot-nd-Mouth
Numb+r o
Hood l~scat
Nunbor Iroctod
Dioaaca
-
0attla
hdbp, Ooata,
0attlo
Shop, Ooatc
wiha
11
21
3O,/3
1,172
1,
1.4
1d2
1e0
164,968
19,61
1.7
2 ?~8
1~3
38
/0,679
X0,613
3.6
4.1
18
18
2,992
721
12.9
66,2
li
30
72,b 3
213
1.54
63
1a6
2
214,460
23,540
2.03
1.1
ida'7?
5
1t7,895
7,145 .
0.72
3.9
From analysis of the data (1'iura 1) it may bo scam that
?act d-rnOLth diccasc in prcrovolutiorwry Russia wac recorded
an na11y a a ooncider'ab1a epizootic, attacking almost all types
,
02 anima1 ousaoptib1e to ?oot-arid-mouth diooaso. In 196
accordin to very pprorrte rackonin, about 2 million head off'
stock' (1,705,322)
uootic, 246,4
were attacked by tho ?oot-and-mouth disoase opii
head of reindeer alone being attacked by f ootandw
mouth disoade, of wh 'oh 118,'?17 died and 32,085 waro killed, in aJ.1,
150,802 head of reindeer poriohed from foot..an&.mau.th didoa?e.
In 1901 a new inter 4 oat- nd-niiouth diseaso
ep: oottti was noted, 'tioh persisted until 1905 ? inclusively, attainin
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? .
?iber (ubcrrs
and CT t attacked
by Y
4
Di5ea5e
r aL.4 tti.?
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E
1n 19Q, whon 2 ~ 1 ~ Q dead c~tt1o , 112,795
W J!3 a other anim 1 worn inI`ec tod .Wtth foot-
r thin thoro oa n i a aorta n 1 ontng off" thy.
mouth di~aao~ ~ M'tc
opi~oatio , but.. c ter 1909 now n~onatio of tho opiootio
Q~4t0
sandwiROl~th dicCaco oproadin With
1t1we b1n, oOt
rt~ dit attacking d conoidhrahlO ares~ of tho country.
o~
x
In L',1O rQot.nd-nIouth diceaso 4ttackC4 4Q,9Q6 popu1a ion
point, in which 3,137, 447 hoad of 1a o cattle Mono ware in-
tnd-mauth diaoace, not counting; other 1ncts of
fcatud with f o0
animt1s inf octed with the di9edsa.
uro 1. Sprvad of root-end-mouth dicaace
in tcsia for tha pQ1iod 1881-1912,
During tha imperiali4t vrar ha statistics on foot-and-tOUth
saase wore compiled' but during those years it wa hardy less
di
widespreade
Some COfC?ptian Of the proportions of the spread of foot-
disease and the 1o:soo caused by it during the war 'may
and~riouth
be imp by the foUowin ofricial co~nunique r In 1915 on
a~~ed `
only ono of tho fronts 24, 623 head of cabt1e, or6.43 percent of
the overall number of head cdnsined for the supply of the front,
eri hed from foot d-mouth disease.
p
hand-mouth disc Ise, like many other infectious
Laa. boo
was a bitter 1e~acy from to old, prerevolutionary
diseases,
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ua~ n ~ the ~.nat year of the oiv t1 war real atru g1ec
iiin t j'0o-4fl1-mouth diaeaco woa not oarriod on, not oot1n
cov~xal dntin aornn ated-with th? tran portdtion o ootad
dock,
At p i nt the inoidonc a off' ?aot" t - ou d o n a oh
cto?k in theft ` by Qornparinon with the incidanao oi} boat=and
mouth disotise among stock in the oountrie o wodtern uropo,
is very, i taken with respect to the overall nnntber of
stock ausaeptible to foot-and-math dsoce in thao countee
and th'~ iit of rritorie~ oh whoso ?x anon this stack i
1oeated.
In tho period 19274930 otock in a oonsiderable aroa oi'
the country was ettocked by foot-and-mouth diceaso. The
veterinary ar &aniation in these years pravpd to b poorly equipped
~
for campai ning againot ?ont-and-mouth di ease ? A!tor this, with
regard to the spread of root-and oath dicaase, an interval at
fctt beg.n3 which continued until autumn of 1935. Ater this
new outbreaks of foot-and-mouth disease appeared, which thanks
to widely developed anti?opi~aotic tend prophylactic noasuros
were quickly e1irrLnated.
Soviet veterinary workers, using contemparary scien ific
data and exporience accum fated under practical, conditions in the
stru 1G egainst foot-and-mouth disease, are continuing to cope
ussesstu11 with the problems of its elimination. . (Prof. Ma~nuscor
s ~`
(Swoderl), who visited the USSR in 1932
where he became acquainted
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w do Lniot I'biOf oI the strug to ~ ainst Aa i~ wr lit t
14~fl ~-h S tvu & n t Q t-ard..mouth
~~iooaso~ sa~c~:
Union now tads in the ?ot rink o too
diooaso trio ~rvot
&' boo A. I. ~kQnorOkhOV' tcrt olO,
load:Ln~ aountrias a1t' ~turvp
o e34 o to y of ootd-MQUth DioansO
flr Achiovonts in
ind othoda ot' Stria ain it in trio VR". ydtTs,
No 1, 1934.)
IIIWTORTOAL DtTA
In orbtin 1jtret2'o srr ?vot-aid-you disoaUQ Weird
~
tion cva~~ern thU history off` the rise
alxtrecaoiy ~p~rse ~~~ orm~
or oot. and-mouth dis so o Doti
ess anion ama1
n
c
The rirst ddscriptiOn of he si
foot- fld-mou ?r ..so~do was m de by HioroflYffiuo
apparently sirs to
Fracastori\irs in 154, in Venice. The sickress which he descx~?~bad
had appoared then in Upper Italy in 1514 (a1~marin and Trautwoin)
irate Italy ire thoso days roprosonted
~~~d this was not accidental, s
orb the loading ao morci l centQrs in drops,'
ana
se in anim 1s and pradu.ats of cnimA1 husbandry, and
Comer
a and animal mnto i 1s, were doubtless
transportation o~ anima
at that time the basic Capse o the 'spread a boat-and-mauth dissaso
This may be assn from the ?ae~ that as early as the 'si~tgenth
oontury thar? was ~, in It1Y a system of spec~.a1 attesta-'
~.~a~rod~c
tioras ~jven by ..owners of animals ~s to the stato off` health o
s.
ihese 1attG1' pon their emoVa1 from safe 1ocalitie
u
-15-
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9,
Qot h f 0f t WC-d ~
~~~ rho a~uo~ o~
o~~ b o~fl' ~~6 Oa~
o )
~~6d nth ~~~ ~~~4 one Lo soh
t ate . l a o~ that fin to
co~~uo a~
:t }vct -f man ~itn
hco o, ~~~o~ o~ woro ob ooh-~~~ ma ~~~
ooWa ? Ir 1T!, in ~-~~ off'
o u~ing w ni1k i~oIn
~~~~1 n cud.ht,ooi' o~~dc,~c occ~~tQd o1
Au
100( ~Ub'&t )
di$o~so a~c~r
h~~QOn~~` con~~~~~ tha='c
tha throttsh
i ~ri ~Q111,1~:~~iiFzA ~n~~ ~~V3~~ ~~
caxad d.a~cptionc which rtorc t1tV
opi~noLcc, wh~~~~ attac
bho rub o~ ?ocb-~flc I~ad
Italy, ~d ~'ranco~ Thdco
~o~.~~h~~~~ ~r?~~, w~ th~ dibca~o
ctiovrnou~ pro,o~~~ono ~t bhc'itcs n the catrco o
~cth- t '+
~ rc COT itooLt ?~'
Eire horo ouchmri~ chap
vrih c~-ou+
nd-~nau~h dise~so tn th?sc da~~ wa a1rcadY cor~~id~~~d
'c cotha ctuo? o.~ thQ oibknQOS in a
h
ot
Y au~
be trang1ri~~~d undor ca~tLtT
jOtOtt maA r aan~t" , Which cold
1S11; waldin~er:
~cc1i T ?ohou~n'
ca~ion3 ~'?m cfG c,nii t O another
ere More even author~ who con
3atc1'&I4 ; and othar o) ? T h
113 ;
ot i~eCt~,ous accr~.bin~ tto
idor~d that ~bio o9,ak~a~c ~vag ~ ,
~ ~, ~ir~'d~ 'i~27; ~x-oc~to,
?t,.~ to atmooPhe~c ~actorB (Huoard an
far i2) ow vor, with tha pssage oC t3mo ?ac
~?~; and ~~~. ~
ny A 11
? ' i .
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__.N
 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
Qt ~oo~?and~~o~`~~ a~noa With
ao't1rf?~ooono
~t~or~ a~a~~~a~-~d i~
woo ~ovoa1ot by n~r fro Avon
rat w q n ttY. Lo ' prt ran h -~ ob~ar~-~~~ono o~~ ~~o
;LnduoQ4 tho oltnoaa
~~~aa~on~naoo o~ oot and
in ntii:Ui b, o .` ic~a~
~~r~r~ o~n~~~ mac gad
In ~o ~toond a of to t t eahY
dUaoCo~W~ a~ ta roiU1t o~ a
~Jt provit~at
p rt oU3at1 j 6ebi on. M, InaiiYt t~o
o
bJhed in 1'7 ?tha t~1terttb1
Loo1:ior, Tra~oh} and ~~imihuth
flA1Ua athe ?oo~Ufld
f
die~overy was tgfjo aoid tn
ovQry the ?t1tarob1e natoro ; a~ th o inc`Stant~ o in~flo`~~o~o
way VLre~ mado by 1b,s& an' tho1ar~
etoknoQe a nnitfliL and pifl
In 1886 ~wnaie:?& anrtaunoad tbab ho had been e~eces~~ul in ieatin
a ca1r w~th blood roin an a ~~
blood ?i'e~b havin~ baon
~'l1ter~d ?~t'~~~1~ a C~~amb~r'~a~h Cilter. bablohod ta't the inoitant of tobaaCO-
192 ?i1&ff " `~~'h'ouh b~C~ifl'~a~, ?~,~tr~~`S ~fld a~~,~;11
r~oea~~o ~~c~~eo~ on pass ~
j01fl00~ tlnooo plants. TY,eee rtm.rkab1e env?e~~~a~~on~ of
9 any
rotrn ,,gin?~icad in ?oreiZfl oawnt-r1o~~
R?~eiafl scholar oanno~
t in ' beitnfl'in~ tQ dtscooo them
though thet'G hay been ~roat tar&l,nee
the iVeSU~atians o~ the Grn eon enti were soon
a~0re Nocar i Rod and others
dot lioated by the trench inveett~
p
u1t of di~forant laoali~ ed
flocr_.and-Inoxth di9aase, as ~ho rep
oa11dd by the m?dive~'ee namee s
f1ictions, was ?requeflbl,Y
` O.nthi'jn'"LJt~-w8tln'rhO0 ~ 8xanthOm,
hoo dm0uth die `G , ` etc . (in~ end ~;rausn) .
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22: CIA-RDP82-00039R000100170030-0
THE NATURE O TI F0 ~ MOUTf D I D INCITAW
tit of this ?ootd"mouth dicaao be1oog~ in trio
rho inci
torab1o viruses (u1trI1,v 'ui~a). Trio quostion oL'
oats~ory of ?i l
of this eauoai ant has as t not bson cona1uoive1Y
trio naturo
sott1od by sc?.Uenao f just as it has not boon aott1ed wi h rospoct
to other fi3torab1o viruses.
The tandin Sviot cc ho1ar AcadQmiciO Ot ma1gya con-
that tho a br?ibuto, i oron tot' virtsos, of autonoI ,
oidor~
ty, adapbabi1ity`, and, LinAfy, ibhoir ability in the
olactivi
s
process of roprodaotion to Ap xoservo their own indiviidua1t1, whin
is manif~sted in tho eoio charactor oi' the cicknos spas cased
b them rovidoc a bests for the 'e koning of viziL'svs as living
beings .. .croorganisms, Such a view on the nature oi' filterable
hoses i con rood also by the feet that there oa st sc~ca11od
t1orpuscu1ar virltso?" (o1smontary bodies) which soorotG speoiric
c
ariigen3. This connects vir?css stiU ciosor with
s
microbe (Boycott, ott, wishing to doine the position of viruses
in tie system, arrived a..b the conclusion that they stand
natural
da o1 the transition from inanimate to animate
at i?,hs boon
quoted by Gam1eya, N. F+)
'rho question off` fi1torab1e' vis'tison was a1ari1ied at the
Twelfth tion. VoterinarY Oongreso (1934) in the report of
lnt~rn~
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Qor1 ch, whQ c oonbi oro that vivo oc vtn cr uniwn .
o the cf his numerous invootigtiOfo of the bias.
1o is ~ bthiwLo 1 of vi oo ~ o~ aco `to o~ver~ cn'itia or tha
thba off` th ?' 1t 4$tr y o aurrcneo of hu o aoUe horn ncrr1
buotaria" ? T 1t i theory, 4 DracJiR a no hao co st boon
cahfirnncd a oriontally b na ono Tha goat mbar of paus
~
c :L:tha tho r autonoin, ~pooiiia tt,ir M? 1 ~ i1ity only conk
rrm thoir living nu.
Thy m6 F i~ to Oo wr iOf 7Cj?i t ~tuIy ?T S PQJ 'J ~~ F7uID
diaoasd (Olitoky, Trabm, d ShoanintO, on the btaio of its in-
vcsti ;& ion$ alto a repsed the assumption of the ' vas
scular nature o ' tho ?oot-and -mouth di tr lo. {Tho ~xnorioan
0 4~'p
nTrtissian for trio study of foot-nd math d~1sr 1t G W3 or ani7od
ca
throu h the Bureau of Animrl lnduntry of the Dopartrnant of
the DS in 1925, conpos acl of Dr E Harry Sho ni of
Agriculture a
the Dureata of Animal Industr t, Dr. Jacob Tra tt of the University
of C,tliforniay and Dr ? peter Dlitsky of tho Rookafellor Ina?titute. }
Oppoeite views thospeat to the naturo of virusec are.
held by certain foreign and Saviot investigators.
?Doorr Fronkel, Jnnasori, Morphy, &nd' others defend` the
of the endogepic origin of viruses. According to the
hypot~?sis
stand of Doerr and his foUowors, vsrti~ee are enzyme-like agents,
ch cause 'changes in the mataboism of the cell itself, as a
~vh:L
r31 of which the cell prodttoes the substanceB of rnotabalism.
19
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22: CIA-RDP82-00039R000100170030-0
"MY Vt"'t o ya thQ ?ot iflvooki ator n , "A&fl
tA A~bA~g~ Whiqh PAAAAAAQQ
bi r6 ard6;1 as c apoai i n=i
of di~~;~no~t;ian to ~
prod xtio of ~q O oat y Oh1y Ln
xr ~t to a pow mode (t1I ho ih'
oata~~o~~ daot th6 ~
{ 1a onta1 a (biooh aal) roAotiofl a
idion off` too vitaka~ p1ico.
ra~u1b ?Lwhich an aoaw
~~to in a aata1Iti~ prooo~?~ ~~~ too
vorr ti~p1o for9n, bha rtioip
ata1~F of and. ?~ho proatiat oX aatalyoia.
o~~i~in~ ~batanaoj tho c ~'
In tho aaoo oL oL virus '" awd the lr~lprod Ctior~'
~ ant
dovo p
c?o> is p1rp1 od and wt havos tho origiita1
oi rho virus tho pro
sb oa~n~ t~s
a ), cm1, tho vir~aa a~
stab tapcfl, tho virus (t ot&1
pror ct r sata1 is~"
i~tvea~~i~a?~r~r
~ or to o ~r noriaan
~n this cor~aot=1sot~ t1~o disoovQ~~
ticu ltr intoroa ~ ? Stan1eY,
$tan1ay in 1935''1936 io oL pad
via~ua disoaan3 o~ tobaaao, i oii tad a ary'sta11tno pro sin pro"
aossing all the pr0porti~s of a virus. Certain invoatigntars have
Boa in Star1ef's disaovo?Y of paraaitia aib> ns
been inalinod to
roots of tho inflima'to nature of viruaeS
ono a~ tho p
the "ntoot re kablo thing in tho
}ty~ov ean~idarS that
got that they do not quite fit thG
phonomofla desa1ibec1 is the r
u 3ua1 a ont epti on of animate anc1 inanimate. The ary ta1lna alb n.
galled an organism. those noWlY
oi: the inosa disease must not bQ
?aund parasitio aid as it wore. on the border betwaon
a,.bum~.ns staid
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anite and inanimats~"
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Doubtlo~sf tt tL oovory o par c tto 1bur n~ po i
thA ro t e of p tho onio inQ taflt oomuwh a'~ron ' hor c the
Aoitio o tho p rti nc of tc "doad", inor d o tAt o
vlruz U but t in no d' a ro o1voo bh problem to
wtitt in ro ro onted by vi~cea - "bin" or " tto ~'
p
pyl, of the basiu of ht invosti at one' ci wii ec tho
,Loot-and-mouth discaso virus ao a hyUrophilic ~y~~oro
colloid.
Janccor, uain ?tho mothodc of coUoida1 ch mistry~ took
th3 vi es to'bc an inoolublo acttve p~rtio1o corn acd o' a
slightly denaturedobulin ant tho ro?yoin containing the virus.
,Jgnsson's o perimo th; tno dohy trot=lon of albuminal bctratum
and as n r 3 ult or this, the aiterin ai' tho at ucture of the
albumina ` did not have perceptlola nlucrtc~a on he activity c1
1
tho vir. Tho author ht drawn from this the conclusion t~rnt
the foot.s&nd-mouth dicnasc virus i an inanim tto protoir botanco
of ,he ?orrnent typo.
atnor, and also olavtyav, as a rest of study of the
biology and asoertaix rr t of tho naturo of the virus, olassiLy
it as a moloottlar spot;s?ion and deny the animate nature off' the
?oot nd-mouth dit oaso inoitant. Howovera the data of these
authors, secured by very limited prelimin try investigations, seem
unconv:snoing to On the basis o:' them it is impossible to
drew a conclusion as to the dead or livin n& une of the
X21?.
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
ant
11 it
Vti fl: i
N:
k
veal ail the
R!tt C) t, rnn:L;L
J t ,e rs:1
multiplY onlYn use.
~i3T w
receiVo in host orLT1
pioperute
G ma1oy ~ N.
Virtu3 Si
T
tt rt;)y,
inho~'eri' :`
The Present?Day:,
esses of Pxents and M~a~vos
thel roc tai'
uiii rE o ~~~ fh ,C l
::td' vizi.iea are j. a state of ana~iosis o
one
&nt
those subrc~os
Ong` nsice tho iisxt sin
a1 oomp1Ob orgw St
o the Vis Problem.
Against Thom
W#.
v:ir~1t.i`rf4
tt;
e
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
Riv~r~ long ego poi c tti ?ao that the r y
g v u ? or bit 1o n u..' ton otvr n zh
vtial nd ti off` to ho, r r4 t
rtiall12t1 jntito tyo: ~atciticr, Acorng to
r ~i livo only in tho cc 1i, and not io th ?iuitc
m or ? rt 1 r
}'O1tt (Jv (oU IWYb ccrtwi r' itmaiub1c tho rnblw~ aV th
?t t th vi E u . Q&krwtc t t+@3 ~ i r# of t @ n akHsM kk titi1ty
m cIia, 1 ~ rocot iitr o~ i u to m ti on tkc but of the r
byci o?c hoiitct1 prop -rt i w=ith tc ?`i~i-i &rtd t u u i o n
n' t ni cm i will b .c cnc p ' ct t c liy otxci ~ c to cu1tivc to
ii u} in Lrti ficiLI1 ii1- o ~ o lip, "Irt ~ bt~orp c t c eu1tiv tc
vii
fl11r~iW IItI my Mtf:'O1ft4:iov, its P?J8ib1 with qu i
O Acid tit)t1 ba ;O boL1~ backw rct ew u c1 Lull
tncI ?o and tow td art i`ic al modI t w*i ehtut 'iiv :n ; coi1c,"
M he pr? gout Uro the -bi1ity of corput~culLr vi:ru cc
( l ~mtmt ry bodies) to c Groh c ocific r t on m ~t~ bo con..
cltcroci proved. At lem t t biity to produce obi otuc hnc
boon proved ?o v l o ph o t.c coil, th vi `Ur of v dohic, the
ycUow ? v r virus, oncI o ~hcrc,
on htt quo tion of tins r~ vorcibility oL the ii?ctivati_on
vi1u~oc t113 ?O11OVvi1 ~ ctc~ mot1t 'O' AChdcftLciLu Gwt lc mtty
c
be "tod; "It is nocoos&r'y at the panne Limo to ronour ca ro4al11tlily
c~
ha :L $i trvivtl:; of vi. balym, which suppn sec thrnt upon tlho
t
Lt'lOf of life some vital principle c#isappear it'rovocably,
term.Lr
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
b 4L1 with aorrf V ub ~n &r4 then
osn,
booQw at 1ifo ~g4fl Whoh th~ aarroa~Vo aubiiu~to
do `~~~ c ~~: ~.~~-? ~ ~ pbn ~rv~
prooi~t&tod wit hydra tm a iV ?
far ~ ba~oua co1i wor tho aot~afl off' o1'&1in&f ? (
N. F. "ToxtbQQk c Modca1 M
rnLtfl ?iO a virnco Qac nat_ vo
T1oXcnssively`
~rn%to h~tnrt;1 0;e cnco "fin
f unto 4cf ror f1on, n: tho
tho TiniI1W dc~ro~~ or or~~~~at~ an
ooc~nw wa hnvo no conoopt o
eccrntict1 ?o r tho lawoct Li- . vn cvaat~~ro? It is e~atro~o~
~ rc~~t~:~~tmo~ttc a~tst~~~~at~oo ' vcn
n~robab1e that a~1 tho fax tho ai t
5
viruo s ti fi? t by tho coU of tho hoc in with tt vo a
aro sa
nc that all a~ it prOp .. ~Wnc 4tjatt is lit%1 to &t4163M1,tdCCd1i tiF1tk
-ciivisian? It i8 P U L that a w pr tcin mad Ocu1 a ar
surric~.entl Lo tt i
It ( 1 y t
F nrtli ? th discavorY off' "P ' tits alb n -r' in
cry form in vivre sjcoe~%;as oi plant: docs not aQ~t~n-
Ei
.,ta~~.na
o Etta natur oii vi '~tsas ? Cry 11 nc virus
dic?t, nrao~ o? th
are n1co sncratad at `tho pre Font titnc ?r?m tho tiss~os
pt otains
with ithis (foot =ar d~niauth &seaso and othar8).
of cnitna13 infacted
n~ ~'ara ?vVBted off' an ~cc~tm~lat3~on of
Theo crY~tal~~ne p1 atQi
same o1dmont~TY bodiO3, which have, he wover,
an nUmbefl a~ the
the ?acC littZe sticks rather th?~n spheroids which is sub-'
sequnntly corifthttalso ?or plant virus" ? In th~ opinion of
A,cadarric ittha ability of e~,omdnta~7 bof.~iee to farm,
~.an (~atna.~eya, ~`
Cbrilpb9 iv s in cr t all na f oi'm bi Ori s, in no wty c an-
tra~t'~icts n~ the thtl1~~~ Fact that a~`
ntar bodies are aim&te microbe&1.
.. ~.ame ~`
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
ii viowo on tho nturo obo aitd or on th
r. oL1 roprooontitivo oft v 1on0 oot mtii'ia trmndo give uo
bai c to Como out oomplotol d do's nitol in ?'ivor' off' tho
anima to rtturo vir oo Cono qw nt1 , thinoit&t o foot-
arid-mouth' dit u i e rn~st t10o b'o oono dorud o "cro tutu", coop otng
a 1ivi t: o'nitc rw1t r,
Th diooovory of t rn inoit t oL toot-tnd?rnouth di iotoo
i~ oonnootod with tho nnrn of loof tor, 'r~osch, d tfh1onhuth.
Th so invol: i atom octab1icho1 hid tho incitant o foot-and-
moi.tth 4~ F3 F3 tf i a L 1 '-pkf R~ r1 m o t ti3 1 ~ +y i11ti1 nfEt rkh kltt3
it pat : fr~R 1y thrO Igh bactoria1 filtbrs. At tiro owmo timo,` tho
virus r~)covorod it tho 'fiibvata pro ors c to ability to o uoe
sickzioas in anirnis suscopiblo o food-nclwmouti disiaso. Thus
the etiology of faot..and.nLouth disea&o was i' rs `doto mined con-
ciusivciy (1497)
the fiit rab1o proportias of thu foot-and-
mouth disoaso incitanb wero discovered.
Thin oubstanc1i ,g dicCnve' of Loofflor, 1~rosch, and
Uh1cnhuth did net so1vo the problem o' the nEiture itsolf ci' the
?oot-end-rnob.th di,ceaso in (ant, bui &U'tori this discovory new
peths fox? ?tho further study of its biology were pr'o e~ted.
iz OF THE VIRUS
terminatian of tho in nitude of particles of the foot-
and-moth di;ease virus is carried out by tiro methods of fi1tration
and t,te,'i,lratone fiat of djfforcnt invetigatore w th respect
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 Declassed in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
tut of pi C o tha virus era dlvargont, whioh
~~o ~ha
be oa ty fl~u~o aXp. aaa~ll~~a~o ~~
~ ba ad B
~coto~
ttr1fl
n " dot i, r t hi him Ln Qpar -
dia to dab
g J? , too
pint ni1tvatbon of
nrtio1as within ? w rafLf btt to mil
ttn~bo or Ito
Vi4 tf t s ti ma iii d o 11a ~:s
a1:b avt['y Eiz 1 bf ord dat orm ficd tho av oraq a wnot or cf
tholos or 1h vir~~ as to 1 m ,, and Levaditi d? toxminard
par
,it tci rtp ? O d if r t!zflinin th 3 j t fli1ude of
f
ptioles aL vbrris uo?a a aipocial.ccrrcctbf foi which
has bean naiiid the ford fore 1a. A ord n to 1 Ord' daW
only ~rnrticb4s of ? t viru i pas t ~otgh ' a fi1to? who o ii o io
than .that of? th Coro of to ?ibtcr? Tho
2 to 3 t.~rtias boss
not p thro i h fibtors whose poros aro tf 2 m
~~~~ i~ doo , n?
and conseq~icnt1y tho of it ij partiolcs is t qw i to 3 to
12 m
anov and-1oio dotermine tho se of particbes of
thin 7- and boas thai~ 16 m . Thane
viru; tie 0 qs may e
xiosubts almost coincide with re;iubts obtained n the experiments
of aLIbbY and ELt'ord phase authors, on tho'b~-sis of their
oachod the concbusi?n that the limit of ultra-
r is between 48 and 13 in , and that it
f~btrat~vn , of the v~ ~'
is c1dsor ta`13 m, than to 4~. In the z' experiments they used
the o itholum and "1ymph'r of ph dice ? Aftc r
Virus from
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toh tYu pp ~M t-hc vir way
prc1itht%rY
me Uur n wopor'ti0n a =100.
mcmbr'Lnc~ a1 Fobs r'c~eh~d
0i,11~~1Y, u~in~ the ac13,oi
ss~eeesss t Fl lta'[y`yr}thu tkU thQ o~ of tip ?~otd~
oortQlucion ~ dimoncior~F* 5
lci~ , then tho~Q oi ctll known virucvc ,
motlth di~oAco vac bra
with th@oxi~opti can of thc vrdc o noli~nyo~i~ ire inn bn~c
The diLQnutor o~t'te1??~ o1 rho ?oob~nd~mouth dico~ac W
~~ 12 T~~ eh 1i 0.01 to 0.02
~
otr ~ ~p~ro;~m~~~~cly c~u~1 to ~ ~,~~
tho cU motcV oV tic n 111c t r L robs
Artor;icon cafl11i,ion, to iMtnttudd
Ae~~or~~in to ~1tit.a at:t ~hc
~ mp . . . M41aro~n~nt off' partaLclec
of palclea of do rwJ i~
a~ the ruo on tha banis of tha ~y~tom off' ~o-ca11d 'tr~ioloaUiLt""
ul~r'~~~1t~~rn showc that ire
?iltt'1tion~ throi ~t~chold
t~ other ~rticleof identicaby compr~i i
partselao haw, b fon wit
: `ber botween 20 and 1000 m (Rivers).
chargo3
AcooVdi to Sochoid, 1 1co Moth'ow, tic avor1 Q
r clay of Vii type 0 i 20 m ; whi21o particle
~.amQtr of ~ari
'
of vii it A x' and are cloe?r In mrt~nitude
r
c are conoiderably 1a ~
to tyre sine of: olements visible in t* rr roScapa Those authors
stig~ttions that the magnitude of
~~,r~ on the basi s of their ir-ve
tMand~r-outh disesa virus in different
p~ioles of the f o0
s is non4tnifox'm and that with the
r'ietiee (ties and variant)
va
help tion mothod it i possible to separate one
of the ~tltra~iltra
variety of th nother' , particilarlY virus tie 0 ?rom
r~ ~.~ of ~
,,i1i: ee A and . Galloway then, on the contrary, considers
w 2
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~Y4t b~ 'tha mthQ4 o
the ~fl.tud~ o ~~~rant t~pa~
a ubli fh any var ~fQa if
and yr ?iw-ts Ot the vitae
s
;t~~da o~ or t~~ ~ V11
o~F study a~ the ~ c
a~M tftr; ~~5lhMd W praa~-~;tatir
suparPA'ar dontri~~~a# Yth
it th the ha1p o a
basis
u mambran?a, Are ~ tha
subsAtuant i~:1tr~-tion ~thrch ca11aidal ~
dater~d_no the ca 01' Vi '1 partiol?s
a.t` their ao~~ant~ that
ti.t 16 to 23 mj. Vus rticlaa oL all three t~pas ~r?v
In aut~hor~ ' i&.t~V parr
un:1 `arm in t~iOir aXparimon ?
wants in bha iii` n; tdda a rtc partic' Was 3
ultra~iltratiQn ~
itudo a~ poticlu or the. rug
~ ~ .
to 12 r. datartnin thomago
by m~anc o t' w~ighin~, tho diamotO' oI thoso
,
23 m 1M, ii: it is assu .mod that they aro sphoricci11 in corm.
Ardanu~a rid Pyl with the help off' the oloctraf rr 1 t'Qscapa
a
obta a smear of Fluid from the foot-~nd~m~~.th
~anod a phota~raph of
dic?ase vosic1e. This photograph makes it p0~sib1o to dotact
minute particles havin a diamQtor of 25 to 30 mp'. Ir all
~
s e mole c33gCs or the virv~s ? aocore
prabability , these particbo
'fl~ authors, the speciric -av ty of the virus
irzg to data or the sa -
i9 appr OXimat elY .4? w
f
pyl asgurrtas on lis investi~at;tons that rho
the.. bas~:,a or .~
aolutionS, or in aphthOus lymph:
f aat.,c,nd?ar~outh disease virus in
Y
is not in the farm o a individual part~,clo , ..but consists of a
an ~.
h individual virus particlO (trvirus
whole , a~gregat a o~ suc
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~ r tt&' ), Tito m n&tudo of thooo a~ vo too doponda on the
toy ortturo a ono ontr~t an a c ou 1 tu2o: nob t t o or tUro
m 0 to 20 Contigrdo tooootio (ri - '?) o tho
vb'u ro eta into ,ont U vtrttl nt p olo ant back ovidontly
tdko~ AU t tooo dates obt tined d the xooult v' nun row
s ivo cny lei tad oonooption o thn moniudo
invastitation,
oL' ptr talao' o the io ~ahd oath diooaoo vies; ooholuaivo
off' this nrnblom rn'wt ba too ob1 u t o' : urbhor i wo tigutions,
aolution
Until mora porZoct mothodo oL pining the virus ?rom the bulk
album a ku i o wI, r t 1d o e'f Edq ntl , tail it i 13 obt ri 4M in ui 4#
'orm, it will bo impooiblo bo solve potiviY rho orobl? oL the
ma . ni ludo of paialos oL the ao-end-niouh ~ti oatso virus
In mItn t~ltravirus inrections special ?intracellular ?ot'ttL-
Y
s
tiohs have boon eiscoverad, the socillod ninc1usian'bodiesu
To s pacificnature oL' theoo ?ortn1ticns 1'CT' many ultr&t~PL vd in-
?ecti+ons hac rat yet bean proved. Theso inclusion bodies have
diactostic signi!icance ' such aicl~esses as hydrophobia (Ncri s
body), t orna disease (the Joest-been corpusc1a), and small-pox
smail ox nhthexia) in fowl Paschen i s body) ? dii Llar inclu ion
bodies have also been repeatedly doscribod Tor Totand?'4flouth
disease,
Gins has described inclusion bodies discovered by him in
the epitholivm of the tongue of guinea.-pigs which had developed
?oot.fnd~mouth disease. Trautwoina and likewise Rt , also
discovered inclusion bodies in the epithelium o1 the tongue a
9M
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
hu~Jth nn-pi. The per nauno in bha othooyao
nimn1 whih hid dovalop d ?oot-&i4-mouth ono o oribct th
that tip by Coco Ai hvs not bore vonh eon-
?ivT Lion by rayon.
art oh# th the help o' u1tr '-Vio1it 0 0ooo y~ o-
aovaWod r r 1av Lc is or the typo of o1oniont~ry vir s bodioa in
tha oonnt oi` foot-d-mouth diQ~oe aphtha. For the tuna
bin bha author rofns from qty eoitclusiono with reapact to
the origin nr1 ntuta or the bodioo.
FTT TION ANA ULTTtAF1LTRATION
ThE) ; Its ibi ty o1 the foot nd-mouth disos a vivus,
as hao baen gd a1roady, was ?ir~'i e tabiih~d by Looff 1nr,
Froech and Uhior huth `boy proved that the foot-ancl-mouth
i
dise u o virus passes froely tough the ~erkare1d rind Ch~nbor1 tin
filtors. With this in mind they ride use of tho virus in tho
form of the contorts of aphthae~~us~lymph"), which had boon
proliminnrily diluted with o 1;40 physiological solution of
sodium `chloride. Thu non-ad L ibilitY of b tctoria trough the
ores of the filters w ono11xi. 1x by The additton to tht filteia+
P
blo 1iid of b. ijjJj
~-
vrih sunsoqusnt str fining of the
fi1tr ttn into culture mods. Tho brosonoo of virtts 'in the f111rata
was veririod by merits of i octing calveq intr venow 1y.
While tha stmt?ngs if tha culture media did not produce
rowth tho calves became sick vrith f oct id-mouth di seaoe with
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
j a~41 g1tnct11 PAM 1 J.u~ 0 # l &T I4 'w ah h
btn t Y th t 'rLriw (?1 ph" ) b Q1 i v~tt?n.
Fracch and U~'nhuth ~1ra praw~d that avax~rtar~
p61 th virue w t n the Ji1tr to '1 c&pAb1e of
~ c ~ ~i~tr~tion
oufLn in aim 11 c1 ~O Pbib11 to oats ,tea'%th di as 1.
Th ma. a tango (ntrnaw.pov ) Xit u to r 1 &t1 tWV6 pr t%11y
~
~ Fop Rt tha virat
Thor ~dlta di' the inv s ;dd Dior ~ OL LO or, F~'at th,
tnii hlanhuh ort tha t ' -b ' ti at? the virus 1mvei bwn con-.
?ir9nad by Hacker n Q r u1 ~, Not to a~tr in Frw~c, d
other ifVf;t41ia4,
'rho . ? - n o.t' to arty in contua3biaf 7 th i dy
i a b his atiso on,ad ether inveitorc. Abo h~o
~~:c~a~,Ir~~v
?i1twact ut~h F kon 1d crandlo vi i iym h rom ephtkmG
t ~~~
F' or a the virus~1ynph ha a bacon dilutod Vrith
pie,
a 1s1 hy3ia1cio1 s?1utian of siurn chlortIc.
tockni r i rd tnnnt, (tho ' }tii h caun?:i. iar an ctw1y o
?oot~~nd-mo1Ath i, oaso was are ani ed in 192 undor thu Miniatry
of A ricultvro and 1she Les aM ws composod of Prof. I. Martin
,
S Stoc1 nann Dr Arkvri'ight, and othors), for
uZ' t jOf took 'the vtru3 in the ?orm of smaU places
{
oi: the a~ithcliw of the w 1s of phthae ( 9 epithe 1 virus" ),
n
ground thin in a poreel n mart&a &n1 them strongly diluted this
with a h9 ia1ogic&l solution o J& Wt1 chloride
~~ ou~d miss w~. ~ ~ i ,
In`fi1terin such vt't s u ension n? ~tiVG re~u1t3 were
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
oht in d. F 1 #r Uofll wt o a&4 th ou 1 s ect a .
G ar in F ~n4 L b opt i o n thr tt h oit obw t b
p1 too,
Tho s of tookm4dn n4 Minoot With filtr ttion
aretifl(t by the foot thatdont1y trio v rug I this we
woo odLiorb d by trio Cif r, w, why oh ii tip no' pr tblo
th nrrio O: h ? ltor Worn b1Ot ko t by tno coU1112' o1o h
o:t` th) oun4 opitholiut~, ' b ogtt ntiyi tockminn :tnd Minnot
lift off' th tro~ttmont o' trio vivuc, For ?i1trntion thoy took
to viru& in trio fo 4t of 1ym~ frog rAimary &tphthao of tho
r! t pig, 1ikowi ivd t pt~a m: are i1ition with a 1:50 to
1:00 physio1ogioa1 sot t on of ooc =iu~ o 1otidc, in this case tho
vir o pAaoeo frooiy iit fi1ttation through LorkO old N n.nd Chambor'
lain F and 15 bduLtas, dch wao oo ?Lc- ~d 7 the infe tiara of
exparirF ntnl animaLt. Thr virtu also pot t frog 1y tht}ough
eit obootoo plates, Tate f?iitrate of` foot tt l-moith rliawaso
virus obt1 nod by thoso ammo inv sti atom cause 1 'the infeot, an
of o p rimy nta1 aim s in dthiti ort of` 1s20,000.
3ot1 orl and M1ib1 r d obtained goo re tu1tti ith fi1tr tion
of the vireo tK'rottgh a Mt nd1er cand1o undcr pre suro of 100 milli.
metorsr A 1:500,000 ci1,1+tion of the virus wt taken Galloway
and Nicolau also used a Mandlor candle ?or filtration of the virus.
Thoy u od each time only now candles which had not yet been put
in use.us,to determine the donee of absorption of the
vi1ts in repeated filtrations. They 'ttsod Lor filtration the virus
MS ... 32..x?
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
('P1 nph) ? m the gno pig and lutod t with a 1 s O bu oy
oo1; tion of pH 7.6. filtoring tQOk plaoo at law pr n'.
Ib their o por m ntu c Uowty rnd ioa u ~ tits; t 4
vi oxa1univol, Thoy prduood inoot1an a' oont1
an mgo with v'ario% 4 lutioiut oi` rya rV r 't r itltratioh
ao wall ao bo oro? Wtth twos and hroe4o14 fi1trLtt ona ? naor
taco mtbindioatod tai' ouanttty ?f vino in tha iltrrto did
not diTtiniah aonsitht bly, whi1o in the cAuroo o vivo ouoaoooivs
?tihrationj tho vir!~s dlsa ? trod oompiot 1y.
Ql1 buky and ooz rn io a . ?iltrt t. or of rho virus through
vrko o l V and N aa~dlos, and aloo through Chtnborloin L, L2,
L i 7, , LU, d L1,3 bougio .
Thoy took tht~ 1oab-'ten1-noubh i ooo vir+ o in bho ?or
o: Cho opitht 1iU1n and ~~l ~phri o a hthho To tho dil~ Cod . rus
w?ts addod boftro ?i1tratiott a bufr?or solution of phosphate pH
7.5, 7?: (i'on' l lit r of distilled orator t1ioy .sod ,5
a~cm'. Tho viruo, oroparod Lor ?i1bDatioil, way ?iI'ot
?ilterod through fil?bor ptpo~, and then again through goit~
aobostOS p1tto. In Clitoky1 s ancX Bo' experiments virus troatod
in this mtnnor pa sod frooly through 3arkoio1d oandiaa and
C1 tnborlain boi~gios, It began to be blool od only upon ?itration
through a Chai*orlain L bougio, passod Yrith c .Cfiou1ty through L7
and L9 bougias, arid, Finally, did "not pass at all through Lll
bougies,
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n U214:1n tVthe at?n4m0u1
r1ts sI3 , is ad 'bdontheab toy t ~
rn ' jr4 r r ~r h fj2tr tin of the ir with
the h3 p A p t 'i Lt tt wi b b hEt th g ely b tt
riorb the virus. A 't r tt im t r 4t Of, whoa to
rt:Uo
; ncb trM :w: j t t i11 wet or r~Gt'ttn r liin ~rY 'j tr t:tors
t'Hu th Fac bu ~i v s "t c r pf 7. ~rrjt toy t 1 oor n ~
h
or o" a b1tf.frr hi with yc rLri ' adoo ttof o' tho
vtrw" trko plc. Af1~mrntton n btruvt~ only thu'ouh
pro1.wf c,rib,b~ gboctJ cthici or c
o ont coitrtaor o ' optonn o or pH
rho e f~ wt tii whtx h t 'o ~-an ~ outb ti c .
aowior thrflr h iiitort d n'irtCCtct has` rror ber!n i b . trod by
veru1 irwn r~i" a kor s on t1i y t'U tin rn rtertai it oLt , tho
do roe of to ur*iLrrtirrr?t r'rnaritiOf fo fi1Lratan, tho cont:en-'
tr tion of h ?iro en ions, on tho t , of the poru of thcy ?i1tor,
thti &Leotric char 9, they amour t o ro Fur in ?i1traU O i, the
?t oc r raturb in v? iMh i b i aoo ompll hod t anr1s f rna11yt on
rber or other con do is which often oannot bo taken into
no
accot.
Abe was evidont1y the fir t to achieve u:Ltrafiltration of
for which he used Haen membraneo i . In nic experiments
..the virua,
c coed through manbranneo from No 1 to No 100 and was
the virus .
camplately blocked upon fi1tra tion through membranoe been
reseuro ;of 2 centimeters, Galloway, for fi1trtion
with No ~d0y andsr P ,
.34
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
of tho viruei hay beon in actaft oarlior, ~ Atha ao o on
rcbrno ' of l' ord
Iev iti, OaUow&y, d Niao u, ta ~ rho a ova
appUOU Q411QCLtC)n prepaz frcm t o paraor b o hor- lcoho~.
o1utioh n' ao odiohi In th oa inveati ators' a prim nto t1w
virua uaoo xt through t iplo pp o?Lon scoot n th positive rosuito
x
bain obt nad. in out : 9 caao f Stoc1ott&;nn and ? nn t, on tho
othcr hand= did not succacd in filtcvio tha virus throuh a
doublc lo~yor of x parcent coUoion sacs
Qlitr ky, Trtwm, wtci Schoonir aecomplishad filtratio~t
thou h sacs af' 5 to 12 porcont ooUodio~, Thp pasin ability
of such a ocodion ?iltor wad inoreaod after ppin it in 9
percont alcohol Nowavor, tht meth ' of filtration Way acknow1-
ad ed by the authors thorn olvoo to bt fruiticoo and inr. pab1o of
a1wa o giving positivo rosr~1 is ? in a soc and ink tanco s whoa they
pronarod t13 collodion sacs vtith another e~malsion, the viruo was
blocked completely. Therefore it was impossiblo to draw any con-
ciuciono fror tnes~ srimonts?
For u1tr afilcraton of the virus dltsky and B0?7 used
Bochold collodion membranes prepared from various aoncentrationb
of collodion in solution in oolid acetic acid on filt?r pnpor~
alwa~I` s passed free/ through filters of 1 collodion.
The virus ~`
In on case to virus passed through, a filter of 3 portent oolutian
of collodion but in all remainin inotanees it was blocked by the
grector percentile concentration of oollodion~ In their experiments
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
on 1tration tbaoo authors wood a b r o ution o phoop to
at pH
Mai ow aoaomp1tohoc ?iltratim off' th of -nruh
diooioo t ou h a i ondi ultrof 1boz, but she w~ un b1t to
obtn conbant and Uornouult, Mora oattofotor rooulto'
woro obtind by }kdrow in Ciltrhtion through ooho1d u1t'a-
filtor. n heir oxpoirhts the rua woo blocked in ?iltration
t 'OU h Zooho1d ultrafiltor o1 9 poroont oolution o.L col odion
in uolid aaotio aoid, arid, or the o hor hand, passeo early
through membranes of 1 pcront aolubd on off' collodion in the same
solid acetic ac14,
oa11oviay anct 1t}ode wing the nv3thodt o1 uitrafiltration,
appllod lrordrs corroct1ve formula in order to determtno the
mos b probable dimansi on tho virus.' Thy work of Ladaviti'
Pike) Krasnov, and Wood, and t.so of Krasnov and R inie, was also
dedicated to this ouastion.
The.. mebhod of uitrafilt ation is usad by inv sti for
first ol` all to deterrnina, although approximately, the size of
virulent parbioles oi' the virus. However, as Modro'w points out
correctly in ono of her worko on theubj eot of filtration and
ultraCiltratlon; "Deterrninat on of the magrituda of the pores
in uit z'af 1tor is for the time being an e~ctremo1y? doubt
r tt or, and the ability of a c ofocli on filter to pass par icl gs
of tho foot-.and-mouth dio?ase `virus depends on many factors which
often cannot be taken into accouritti ?
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
a o the q w t , Qn o;t the ?U% el: ti tgnb
tho time bQ n canflot bo ~n wo Q
off' ~~ot~~uth ~~co~~Q for
b' the m thQ 3 a nl It atv ~
cwFmAbIoN O1 T* '1fU
at~o~ i &)pli~d widoly i
Tho motho t oC oont.ri
1o~i baotoo-
o rc ipitation o1 vriout p ztio1o~ n
th p
ca~. practico : ~o~
of or another. y tiro Demo mrthod cmrtatn invootigatorc
o~~o oucpan
vs been atto1nptin to preAipi Sato tho ?ooti and-raav th diceaoo
t1Q
d thus prove itc eor~u c"iO ntt ~z'u. Otho~i investigatoro
vo z
tho method o cQnt i u ~-`t on to obtain tho
haY?~ boen trying YLth
iTt a yore or 1c3 pare and oonccntratod form.
E:A or ?iirtont~ have ; hovm 'that to p'ccipitate the L oot? d.
,...
from its mode by thg con rift al method
mouth disease ,+rizi
r euc ess l nor anyot i o yet. This, o
has in Qssencc not boo
cours4 cannot serve as att indication that tho virus in theso
in a dissolved tatc. vidontiy an incitant of extremely
cat) Wa~
dimenoi?fls is involve:. Ir r idon aU1, 1 alter grid certain
sinal1
otIi? p`sicistc consider that the method of cen i `u~at~on is in
genQral unfi ated to the preoipttatiof of such email part;Lcles as
the ultravirus
ADSORPTION OF TIE VIT5S
The me=thod of adsorption is usually applied ?ar accuntttlati?n
or tho foot~afd mouth di easo viru,
37
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:t ~uaaeedod in this way in more ini the
QonoCn~ratiQn
off' tho virus 1AOO'aidf ThQ odcorbad v ru etn be alutod brok b
oh n n the QoncOntrgtjan of hyd o on ions, Tho proao off'
sdaorati!n and utridtion of tha vino OVido 1y' h?~o in itao1'
no har o t ct Ql jt, ChArCC q o1in~ I3UWfliflUUt hydro do and.
bwT n are moat Q ton ueed ss adsorbon~st Charooa1 io a , oQd
ad p bent, cnd in odditton with this adsarbon tho pH oL tha in diwn
has no substant t1 atot on tho proaQSS itsolf of adoorption of
the virus
i or hdsor'ptian of tha virus Oin and Krauoo usad kaolin in
aolbinationwith contriftgatian, but thoy did not cuocard in thio
, nor in ioitifle tho virus from its nediw, ,
Abo sbcooodod in adoorbt tho footand-out diseaso virus
on o1in3 `dnd picu1ar1y on "oma1" cha'cot1
In ~abmcn~u exp rirnontc adsorption of the virus neitbor
succeoded with kaolirn, starch, nor r'ed blood corpuscles. Mo tried
to use wood charcoal but the routs wore likewise noative.
Va11 o Ina Cara sttcoeoded in adsorbin tho virus an red
blood corpuscles, ark also on living and dead microbe bodies. The
tcthorbir abilit" of rod blood corpuscles was proved also by do Blieck
and ankol.
on the basis of their erirrsson r rd Maitland reached
thconcision that tbo erythrnoytos of sheep, guinea `pigs, and bid
cattle adsorb the virus in sr 11 quantity and easi1 r release it,
8
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l goo awn hdo41a, woh devb thou in
~lkalino colution (kaolin adcorba tho' virus only n naut~al
iw)?
Aaoo'dt to uduwoy~ &icorption o' the vua can be uaed
as mothod off' aanoont~otin ~t This i~ oocont1a1 'W WOO' o
tho ocenao off' the v rw in pop & i~ofl whtoh hi wov d to bo
wii'1orit in inoculationo. The a oacco off' thin motho is as
?ollowc To t1 m to is being invooti atcd ono adds tho adco bonb
aubctanca, then collccta the prccipitato and introducoc it in a
suacoptiblo ani 1. by thi thou decoct on o' the viri.c hac
suc;e?ded oven in a solution of 1:10,000,000. In this ao1utian;t.
was inacttve, bil? conttinod viru1 ii t o1emonts.
With tic help of the adortionnothod cetcin.n~rostaors
hive attomptod to tao1abo the virus ?rom 1tc medium. Mono of thoOo
inveotiSators has succcodod as yot in doii this (Waldmann and
Trautwoin) r
Pyl succoodod with the method o? adsorption in frcoin~ the
virus, ?rom the body a1bumino to tho cent that the albumin producod
d nogativo rerction~
Jansson, with the goal of purging the virus font the body
/buns, applied various methods of adsorption and olution, but
did riot succeod in obtaining it in a'`pure ?ortn, i. e. completely froo
of l?obtiinso Later, with the /help of a special method of adsorption,
Jansson suocnoded in obta.ni ,the.?~i`rus supposod2,y completely free
of the body albumins. As an adsorbent he usod rpsu~m
sulphate (CaS )
calcium
39
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oon ? ~c w11 oc thit s4t1 th0 hip o tho mothod o
Yan~
tho bUi1fl t i~ UUy pooa bL6 to
ahom~oal proQip~tt~t~Qn 0
frog the v 'u from tho normi1 p
rho viruc ~n puVo ~oA comp1Rtoly ~~oo
'4th ~o~p~at to obt~1 -
d~ radt intart for too a,~udy of ~t`n
or tho baby ~1brnc i X01 ~
b o1e y.
ato c I ohs 'i t flincfl, and ah-it-
Tho pash jti
Joncani with the ha1p o ? the method o 1 orption ?f th Luc
with alunum 1ydro do (A1(0H)3) obtainad a "comp1w 't foot?and~
vlrU thato of itiiflUfl o dAo, pococa ing anbigantc
mouth dic~co
proporttoci
cuLT t oak rr} VIkUS
c ?i t` atto~ ~ta to cu1tivato the ?oot andMmouth disuse
Th
virus wesa ~ndorta1cen b Loofi1or anSI roach (1897) and also by
by
Loeff1or (189a~190~), but in all caws neati~o
:th
and Vh.oth
s wore obtatned. Otttivatian of the viaus dngaged other
rasu1t
investtgat?a j, also oco of llyt pfe ffer and Or t o1, Hockor,
~ Hsu
aiiard and Ok~agoii and others.
t s announcement in 1922 that ho had succeeded in
Tietze
ltivating the viz'us in a 1tq4 artt icia1 cu1tixr o mediwn die-
cu
covered by hiii (I~artt brath` with addition of 1 to 20 percent.
~.ns_
sores of 1ctrge catty.e at p1-I 7.8 to 8.0) way not conC rmgd by
,
further tnvoStigationse' .
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?w
'h Ic,tamrcwravon d~ceva at n'itnt o;L'
~iI rabtoQnnnVt.tarc hn
f dot,-~.?~o~at~t ~
oh () i1a } w cor V rm d by no one. I h wn pre ar d
~
T1' ~ froth with the td~tior~ of
p car c nt 1 Q' how, > or P n:1 3
occh with t4 yid of } hic r tra?Yloi t
,_ : c th rcwo Vtf pawcr of the objactiV dice
which da~~blc
~j1 tho IIc~ 1jIt ~ ccc c acr~cd s short ctick . (I lcr
coVod
citwAt td S:rl tV. Th authors r M1t(d the Iltncitant"
ter, p.i)~
di#ovCI cd by them Ln t i. Z % ' rl - f Ho VF 'i 0 7 i S A 4
~ ,.~npriwaw~
? r the v 'iticat a~ of J th n's acid i'rocch l
~poci1:Liy crcatcd
(lic oV y a ~tttifor:ltatl.h rcpr cntat Vacx o cu'flL1 Vtiri 7
z
~~,.
c~enc 3 cht1 Kin) did not co'irm
'':caitcr
srl~
#cC,V{W or -'roactl aaa u do r cnt1y ,g wirtnti i,
t1~e di.~
arlci , sla Vin studied th Virn cuit c dcccriticd b the
'~ra~~tt~~r~ ~~
aut11crt3, did not con.V I'm it. S ab~ncnl c and i racchc - ctiocoVc wars
chcckad by the n l th cortni iari (Bcdcon and t~ait1aFrd h
192, 5tackl trn and 1inne~t in 1926) and by tkccf Ain wicaf co uricSiOt
rtd ooa~. 1927), arid. n t2V ' r wuiti i 1v tr ib1Y
obtairiod icy all cases
De Blioclc end Winkoi cultivatcd the incitant of Foot-arid-
mofrth cliseaav in culture media of blood serum with the addition
of sugar an n, and also obtainod negative rcou1t~ eiland ~ ~'c~i
( ted1 atlnatlnccd succass fu1 experiments in th(19221926) reps ~'
twand?mOUth disease incitanb in a corp1icatod
ct~,tivai~ion of the ion
s cu.1t iY pre ` red by him, but the compo?itien of
~'a med~.u~n specs1
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jug oultur
Declassified
2 if K 1, i t, t' L 4f1
ho n1ih in'v'octigt?r i?
tho m?thod of ttgeuo oulturoo to Oahu
f~ot.anMmouth disease . (Oneof the
? in :t1 itit
Wr1
'# ,' k
~ft'r I
'n r) 11~ d
iwod~.ic1;. 1l oC vi.ru
bho foob_and*mvuth diseao 'izcue with tho td r
thod of OX LOZ1tItion ?~ was made in tho 1J SI
the me
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1~ 7a t 1thou h the tot of roprouo? ion nt tho v1rus, rath?x
tht i itt ourv1vt4, w~a hit oonvlnolr y provod.)
ootootian with tho apooo poo~tlirtioa ol" tho
Voot-ostd?rnouth di wioo viva the .Mtn 4t introtw d oortatn
o}t oo i tho rnobho%t o* t atto oulturo~ t l ~ ~ tbryoM o
ti auo o tho ui~wtt pig (lips, ton uo, a tr iti~ , hairi part
or tht7 sktn)orvoa as trtor outuruodiutn~ In uoh 'tiaauv
ou1turea the"i~ln~la proved tho ruproduotion of the viruo,
suoooodin in pusain it through 17 uuttooaaivo pn tm oa and mt tn-
tt tnk; it in tha dub joot 3u1turoa for the dur tior of 10 tno than
The virLtlcnt3o of the viva in the prooosa of oultty do ,
a town by tho titianda' roan roho~, did not o rub o~ Thu
rnrxitnutrt increase of the virut~ in tho rttaority u Qataoa wo,o notod
by tho authors on tho thirct or fourth doy ol' ou1 ivatian to tht
thorrna~tat. Tc quantity oL virus tnorutsod 1000 tiri during
this titnc, end in on cr~ae as tmuh as 10,004 tirnoa. Tho r it tuat
titre or vitus which the ft itiutids tttitt I was 1tt04,OOO. In
oxp1 tud tiaouoo of the a4u1 uinoa pig, i iarodac o1 the vices
w~a not obaarvod. rho authors likewioo entbliahcrl that the
dorco of ihorua~o off' the virus door not dopond on the ol'taraotur
of growth oi` the tisc~uo itoo1ts On 1ivor?tioauo roproduation o'
tho virus did nod suriuood
It1 ouituros of tisnuo off` the chinigun embryo the virus d3.d
not tnu1 ip1y which the M it1anas a sooiato with the viruoid& L
Obntion o:f: the sorum of urnbryos (the chiokun, ~a is 1ct otiwtt, is not
suaoopti ilo to foo'baandMrnouth disoa o) Th r 1ikowi o Boca trot
43
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1pfl(t on tho pH at tiw r 4 Wl tnct, n p pbabiitty, ie
rttta of the ombryon M doquo
b, tho o~ooi~
Md or tho ,tIflt t At` tho ohiako ti
deb tho'olcVii tvotri4o s Ta 0g
Tiro tt~it11tuntla u
ottbio oonti~torc oi' em hryn"ti oUQ oL tho uir t pig with had
thou adcl~d from ~ ~~`~
boon 1rounci in aroliminory to 2.0 vubio
J
~~~~~E ahc iot tioocprute
oouti Coro off'
o Coati .rfor 1. hours. Air this thoy added to
do~ro
tiro t ot d ombrY onia tioou? oubio oot~timetoro otiroah iivor
~
p3 aEr%a off` th uihoa ;hid and tho perm cuonbity o Tirod' a ooiutions
o
~
The.:. ombryonio did nctt oomhino with this mixture. Quittvatton
waa oupt in' a ti~at~otta t With tomp~ratt~r? o~
oarri~d out in Car~ei
ood out tares wore atso obtlined in Roux
~7 aarooa Cotztirodo.
o ou~.o o etimetars wc-a ploe~i. Tha
in whiort up to
was geed in the ?4rnt of viruz "iymph" in
or,i~inLtl vii us material
M/50 f, ~~ ~'ar ooiution at pH 7.6 in dilution or io,
phesphate b~tii
ttr it?st riiterin i~ throu&h a i'iitor. This rn thod oC tho
proposed by theta for muibipitoatian of tho boat-and-
t s
,.teit'i ,
mouth disoas~ virus in the presohae of animate o~i1s, ads ?'aui~d
tton in the work of rrany other invesiatorss
briiiieflt oontr:
Geoke in i930-31s end kows~ 3tro1or in `1944,
xN
aarr~.ed aut invoctt ations oh sttdy o~ tho tnothoct oL'
tissue au1tures applied to the cultivation of the Tot?and"mouth
a . ,? r.
disoasg virue?
ko and Stria ter usod virtue '1yntPh" from 24-hour primary
lea
N 44
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~phth ~ oL the 4F if\1 lflRtt t aMtt I ?4' Q41tivut1oTL
viruo~ huyuted the ruo with :2O or 1s50 btor
ooiutiofl of phoephuta pi( 7. tnd ttin puood 1t thVauh aoit
iiltor. At t vauo for ito quit v+ tien tho uuthare uood the 1ci
(Ottheli 1 titaue) oD the erwyo a the uinou For tha
as purpose tnay 1o uooo or 11 pot of un Q tbrye. Aa
vrtltur4 rdiutn for tha ti~otw au1tu2'a they uvad b1t~od p1&a of
tho enim1 V~'orn which tieeub for auitiv tiah` of tha virus hnd
baon taken. Strio tar oleo uaod norum of the uinott piffi t a
out ure n~diwn. The embryo fluid whiah w> a addod to the tisouo
attlturo i'ar sti r~t1 tion o rawh of tho tieauo itao1i wart propa'od
from tha w bryo of to a~no typo of anirltu1 train which tissuo httd
bean ikon 'far autiv~tian.
Ooake, `in oultvattn rho taoban~1?inauthiavaeo viroe on
tho tissue of uinaa uvod a bryo tied oi' the o nbrya of
the hen instoad of Qrnbrya i'luid trorn thu strno typo of r initt1, but
obtainod gaaror~ly ootisfaotory reou1ts.
'ronko: and Viu Vt to ion auiti ted the virus ort tho
ep1tho1ia1 tiooua (skin) of ombryoo of cattle. Tho nulttu'a virus
whioh they obtaind i this inatanoa pooseUod etron~or antigonio
proporti?s for eporitan?auely suaooptiblo aniralo than tho sang
virus obtained on tieeu? o ombryoa of the ~uizoa pig. In expari?
ments by these invo ti ators, toot-and mouth disoaao true of typo
Ai after 27 pasva os in tho of uree of 104 dayo, at a temperature at
37 de rods Conti i'ade, did not ohan o ite type properties.
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In e oimot~ on oulbivtloh ofi the v1ruo on ti~ouo ofi
hmp b oed results wOro o,1so obtained. Ao oultu'o
no~ of the uhlohor embryo with
oedt (1) p1m
~di~~ the ~ut~~o~'o u
o' irod' a liquid;, (2) serum of o tblo with the
the _additlAn
t~on ot 4.73 eroent u, xar?a or () oatt~.a a 'u1f With tha
addl ~
~ and ('i.) Tiro0 liquid -* it X11
Alton oi TirpdP 0 1~iqu ,
od'
Qaoe~ their obtainad food rec1ts
In oecin the oulture viz'uo an o ttlo aid hake byc~ans
rtifiioitil infioation and with su~soqu?nt intrav?f1ous injeotion
of a
to viruo it ivao establiohe~ that the epori cnntal to ntL 1s d
ofi
fiaot-androuth disuse, ark ooruna obtained firm
an immunity to
thim pps~UssEd high ti~-b x1y' ooi tunt. Fre e and Vtu' Vavaran
stitudo !'Or ho ohioken pl om&, whioh they had
at~e~~t ~ed to sub
usod tnatoo4 of oo1f plasma, age,r'acar (0.76 p+ roont s car?acar
in inCQr In the prosRJ~1o9 of Qcar-afar growth or
on
~ s aoluti )
tissuo3 took piaao very slowly, while in the presence c1 ohioken
plasma its ty be vary well oboerved.
Aaoovdi to 5tr o /errs data, in tho Riams inatituto ona
tro,in of aul~uro virus was maintainod, with the help of ticouo
s_
aul buros, or two years, f or whioh it was propaated through 118
~' .
o aogin the virulence or tho foot"
a
pdssa~en. As result oi' thi p
ndMmauth d ro a ohed ma nituap of 24 x i174 ,
ais?~se vir~~ s (~
ip,stoad of the iiii ial virulency of 10G, .
In multiplyir~g the virus in tissuo oulturms 5roat importanoe
i attached to tho tompsr ttUre anti oonaantrat1on oL
hydrogen ions
: t ty, .5y f . ILT'? lh. t !4y #r~`~
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o L the med1wn in wh oh the a 4 ro octrri d o t ? At
47 do see Oanti ra a Beth vi 4 ti Lw
to e a ~~re
Tft4itp1y while t o tomper to dl' 3O de rdo enti rdde the
virus U1b liao very e1owly the i ue tU~ et dohs not
MI;11ti 1y. And, Final .y, ut ter perature o ' d de reoe (eeti#
ratio noithor thu virus nor the tiaeuo mutile~ at a11. Ae
far as `he ooneontrd ion of hydro on ions in tho n ti it where
rt 1tiplieatian or tho virus bukoa olaoo ie oonsornod, pt 7.3
~.~ is meat rt~vorable, 4 bo1AW pf the virus doer not
multiply at . all. In oht in tho pH of the u~iiu n 're wo&k-
alkalino to now rat tnrltipiioation off` `thu v z uo i or+~aaoa
Pyl d vbe to autivatic n o1 tht virus in ltipiyin
it; in ti9oua eultureu, u1 oxtruot oL sorntnai 1oxds (Toynait t
factor), and also oxtraot from thu opithe1iwn of tho piantar
surtaoo of the oxtrot ition o u ioti pip, Beth 1i@ whioh had
dovulopod and those which had not devoiopod toc t-an&!-mouth diooaat~.
1towevor, pr~roupti'bio inoroaau on tho virus which oould bo at tributod
to tho r po ifio notion of tha oxtraot- dould not bo opt&bi ahod by
the authors. Thoy notod only tho Tavorable notion of tho extraot
on the pH o the rhodium itoo1L. Auer dddition of bhe ext ~aot this
rern irtod at a lovol of p 7.6
fnnkol and Van Vdvarox cultivated the virus in a Fluid
mediuin in the proaenao of fibroblastf t't bbit 1 ho s, ~nc1 balvso,
but tGy ob'bo.toc r.e dtive rp~su).te`.
Fr:,i c tltivatioi o tho' vii'w 1d be utiod f1ibrablastr o the
47'
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~Ultiipl,o t MY% of the viral.
Ttw foat- n 1-tfcUth vtrL, how by the iiw eti-
t1tio Q $tri~1r, the fait/ ado, Fromoi ffd Vin
its beet of aii in silted upth1
Vavrer~, ~~4d oth$Dr ~, ~-uitip
t Ubo'.U ex eriM fltD ? ~ opithe iai aoi
a
is~~je~~
tha noy, anti sta t ry 1 rn1 pravod of
c~~o~~t~,
.
iitti advtntatGo Lor ct lttV tiol c tha virw.
k the tilt boirL, thorn r i it itvo~ti atod tho oxtrciu iy
het
intzo eFito wham ~xactiy ins bisque ?itur' tho
~,~~~t~stioaw
ttrcs sUitiplic i isidO the O11 itsoif r r of it i surfKca, ?r,
~.~
parhtpc+, only in the' to c1iurn in tho pro one a ' oiootivt tissua.
nctbst~noc~s
.. ~~rt~ c~A to who?~t~er tho cu~o come
It uaso ion,ains u tio
off` tho virus itUiife o t , cu tho botsir ` of
ossoriticwi td rho iifo
h~. reaoho the c?~1t;~tsi thc-t tho virus ovidet'tly
,~t~i . at3o~s+
~
ltiuiie ittidG thv ca11F, thoi o3vos and that its znu3tip1ioation
riot, on growth of th? coif thomolvs, eJ roverai invdst'-
atars void, but from tho aotivitY of the iattor. Tho pruasriao of
T
tho virus iri oultarO s outsido tho ti a cue awns of vt? eviuonao
of t niultipzic tiori. This carp be tha result of its ditf a ion from
3,s a
the c i1 into the surroundir medium.
lnotituto the culture vices` was appiiod'to rho
In Lho Rioms
of cattlo for tho purpcoo of obtainif5 anti-font"
hyper i2ninuniza.tion
th' diaeaso oorum Eariior virus far the gale PUrpo8o wau
1d ~nou
48
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obtftin 1r tUfl ho o, Whinh` aon i orrrbiy oon ttt 4 tho who1a
proof orr(1 irro o 4 tho not o too ~rrt i`oat?anrl^ ~ ~~h 4j ii
o -rurtr produa U. In the Rini tituto~ aooor~1tu to Strip xor'
r~au~rorr~or~i, up to X000 oubt?o o~htr~~r Qf o~~it~ro viruo vatth
o r 3 oQo, o vo propurod
trio:ior? and s xparim ntt11y` t trig izt 4 a tt1o with
i rfr
air .tutu vi x', but ~i nAt obtttif i`'Oh it any~rt(4Ytt, o1' )C j
ir~r~tr~nity, ovrt to rnturai irtcrtir~h. ThesEr rortuit nra in optii
nontraliation to tho t?ta oniio}ti~ii a thf ouituro virus i
rho drn nr4tituto`ior purpt i of hyportuw-uri :zatiof in obt inirt
tuiti'i'ocLnd??tour i itu i i ui ity o {~r .
?roitki31 ?nrlki VUJt Vaveror oir u a in orri tivatirk rho vi~us
ran the ohoriar&a)arrtois of tho ohiolrarr ornbryo (Ooodpctirturo and
Woadrrr~T't riothnc). For tthi pun;poso they use ohiok~rt+s os
after r- +~ .rc~ r ~.r .ntnrbrt inr L`or , , 10, ii, Sri 22 doyu. Ao
a xesuit of their iswesti atiorrs tho auhort oouid riot oori'irm
tho nuittipiio?tt~i rr of the vir~t on thn o}tare oc i arr toi of ohinknn
embry'-s. in their a driri nts the virus kupt its viabtUity on
ohoriaaiie,ntoi~ i'or only 4 haunt ~n all, at totporature of 40
do ?roos Corti r de. }Iovievt r, in two oa~os they sudoooded in ob.'
tart ink srtboul Lure s or the vir'u s.
cssa es ~~ nw' a c l~~r~ed sowiri of the v !u oz~ tho oharioaiintois
p ~
o: a ohickon embryo, and a 'ro. owiti u6 to 4B hours a~t~r p3,aaing
tho viru `ithe thermostat (Certain invostiatore.
CaZ 1owo f
49 w
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Por r tUo, u iir the arw method, on tho other hid, obtained
,ositi results, s.nd uoooodod in props. atin the v rug through'20
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Qh~r Q ~ 4'' QhIok 8 QtlbryOQ
1Mttaw rt Q 4r d ' ' an
`rP ~i1'ba' i-nd Va~tru141aY~ dav~~a~ad
R flUthad a a~11tVta 1rcV1 with pt
th pa ianox -afl o ~134bia hor /. T oae
~c~,~ira~~`~,a~~-~~~~ r ~~aad an s
tha~+~ ~uqaaadad tau1tt ~
c~ y 1W e~roir,a~ SubUan~'Y
otho) an yaaot aQ~ tr&
aparntt i thQ cu1ivLt cn of x'tUC~ WarI) a1rr ?d out tai
bavorr4 oroi ao mtriofl
dieaana virua on yott oai r
p opp o t'&1 DuL' c~h ~cn jttany, on too baioff` th oit' axp ar~-
, ~ or bha ethor ~}irf1, ra~Qhad ~6a~rh~t
m':o~i~ with b~~r~~o~ ~~. ao~~ ~
d~f'~a ~a' Ii ir~div tlU t t tfloo~ thosa au oro
~~an~~ adna~~o~
~uoLaodod i~ rho vir
urn ear "rid yea~t'~ (Tarttla rbra)Thd PrO~o~-4o of the vet ua in r~uah ~ymbidtic~ ouitur" urea proved by
rkr~ i~c n. i62 dj4jjaii. In an4 va~a tha
bhotl rtcttar 60 onor~tio
'E ~v~t~an a~ rho
o in abb~,i~~fl au ~
a~thor~ ~uocr~odc~
io~ts with ~ta_hila~aoo~a~
dtm virus ii In th? ~c~o~Nar~c~-snou~~ di~o
doo 1aborabory of the 1asokh
Soiontif'io Ronoat'oh Vot~rinar7 Instituta (NnVi) oorimenin the
aau~ ad~.1~ adnorrttto 2i1'bar' $ and
n~+~~-~vab~on or : the virus o~ ~
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
uk Lov ' ~ithcd, with
vQ~ ir
wi~h R{dit~o@' 10 p54
~ ~th~ ~ri~h yc~a~
aiu
wore otto~nr~cis On uZ+ W tai ~ s ~~ ~ur~
ao1~ c tho r~ ccnao o V the ~~~~ Vr~c %I*O~ of
_ oo~ ~ihioh can ~
t})YE the odood fl'~t,
~ In ro~catod ~~a~~a~~~~~-~
o~ tho vii~~c, cnd nAt
;~ rcac~t~ wcro abtiad~ 1
v: ~pci~1ad by
ori~onta ar ;~
r~o~~ ~~ o~ tho~r
~
-
~ryi
rho q ~
th~ YirU to tho aa~incait~a~
hox' by tho hiCh r onflittvty o
the ~t~
j
~ tho ~at~ox
-t tit, the ~ t c~
o~ aid in th? a
?vit1y doit~ivra
vin
ha ~
at
cu1tiV
to u that ho me thod at y
o
It go~
is ps i'ii~~t of ~~~ ~~~
,~,11 and a~`i a ~c~wn
turiy of the hia1otioa' ~rf~~,o~tioc oi' tho vdrU.
o
RSISTANC OF T1*) VTkJS
1 octab~~shod
~c bev1
c
.. hd?nauth dirsot wire, a h
rho i coot
high
by ~ rather
tori~cd
i ataro, oharac
#.
c i Voo
~ h o3wa?chem~ca~ x~ac ~ r
by ~u r ~tanco nerou t ago
tivtth recpeot to a1t. Ci -dt a p
tho ati~rrauz~d~n~
in
e C' vaX'iouc raotoro
t o
ar-~ a~aao o~ thc- prn~or
0 0 tho virus dopGndG nAixlY
~~diUSn~ ` rho res~~.~ta~a ~ h~hoed
~d oe1ta~te, p
whtoh it is ctuat
of the ~ol~iuu- in Tho de~r~a off'
ine c 1iva, and othora).
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
V %I1Qfa~ Qt the
#1ItW1 or o ~ V~ A' bQ4,
Pyl . IM tho a~ tthat the v~ro p~~~i4 ai
~o oo~~vet
~ its c~na~t~~~? a1~~a~~ do~ro~nix~
~
bat;j tLblCttirC* tCCQ~ bi11
? ttr pur it a 'bady ailla ~t
icrd, in st>~ :iititk~ (.,.WC. ~r ri ct llt~I~~J:
it r1'bhG .Jut
t, ' -;}t;;' Li k 3 t`CY t1 1~' -' t! '~ t:? "Ffs v
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? of n'g, often nat ?l b 3 Qt to ~an dare t or , whioh in i ono .
n~ fl* Q~t the ai hQ ~9 Fgo~tiu o '4Q ? ~ u h ~r4
tt@,I
tarry tuvaati atom (oaVf or and t`roaoh, Feat &nd [)rooohoX',
~o) notod long ado, eu~a.
ffit, ~~~-1?can and Traubwoiil, Athr
o
qvontly aravod uxporinwht fy, fat rapid a IterrUata pae ~- o ono
ao
virua in v~oua t poo o~ anir~ala `o~oa~ptil~~o to ~'o~~t~ah~Mr~c~oh
y
diaedoo eroduoos t ihtonaii4abor. of ito vtruiana.
oo ri'1?r nd LroQoh auQoa dod i inQroastL o vi lenoa or
the virus by utana o ait?r ate paaea o of it in aalvoa and }ii.
aldnn and ' rautwetR attained tha sum raaults with aitarnate
pnsoa a of rth virus in ~uif~oa pie and whioa ratc. ~urhuiy intro
od to rho ranke f pao ~ unir a1 tho rabbit ae wall, ~a 4 t nn
dua
Trattwoin =Yraroaod the viruohae of tha virus for oatbla, but
and
ubscq uonti in ardor to cause iniaation with it in uiuaa rims,
tho o,uthore ~tati to phso it riret through the organimm of a hoc.
Many investiatocs, partioulrly root end i)resoher, havo
establiehod that prolon~od passe ai' the virus in the Dame typo of
mil inteneifios its virulenao with spout to the ~ivon type of
ani
animal while with roapvat to other bypos of animals it appears
weakor. Rowever, this phonomenon is tomporary,' and ho vials, dae
to its raptd adnptabili?sy, oan in. one or two pdaoages main osta"
.
virulonoo for liven typo. Ernst and nresdhet' Qotabltahod
bliah ins
also that sass a o? the virus iii. animalls wlhioh had in preliminary
p ~
boon i~tnunizea (passivoly or ao'ively) leads to the gradual weaken-
in of its viruleroo and, in tho end, suoh virus oaaees' oompietely
k c
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
tQ onui~o iflAat#on 141 i11Iio ?UaQoPtbio to
oot?~d^r~~~h
~ahiawi~oiatoinn 1027 and in hanovor i 2O out-
breaka t' M @pi~oc:tia oi" foot- ^n nt L dio(1aao wore ob
anai~ hot ? did not ~?3' ad to oatt1o avoh in caeca wh ro both
typos o#' anienis wok in the came bu l4in ?rdutwain
~ ~ ). similar
phonomonu h vi ? bean rated aovoroi timo~ an vermin ? rtaa in
our
gauntry, whw foot-an ~i o~ith disease oi' oatti o, in the nbsanue
at
app'r me prevontiv lustre ,still d ~iob oprehd to othor ti
moa
or animals auaoontible to foot-and-month diseases phis unique
"se1eo tvity" of th? vir~ta for dui`inite aype of aitimai oan be ox-
piain c1 by its insuffioie~ uiru1enae ?or othor'typo s of asp
whioh hti been t}te roauib uitor at pro o go t paaaa ? in :tho
~ ame
din of animal s, possibly, f aanaoquprioo of tho mu -abilit
y of tko
virus, whioh is inherwit in its very bio1oy.
Such soleotivtty of the virus on be ifluniced by other
faotors which in oohditiona of tho nab4tral dovelo mane of the in-
?ootion cast boar on the entire opiootio proaesa as whole, often
not for din the~iselvas to any kind of aooountjn i pthatics~a
Many investigators, (}1eo1Cr, Loefflt3r1 proaoh tnd Uhlonhuth
sohipp, rnot and ireaohex, 1 1rlmann and Trautwein and othor~ he
attomptoci to weaken the vii us for spontaneoua1y 8usooptib3e animate
by Mean of prolonged paaed~e of it through the oranim o nnima1s
of tho flame type. Howovor, o11 thu invorstigators' att?mpta in this
direction ended unsuao~aslly in the Riemn Institute there was
one strain of the vines which had been oropagated through uiilea
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
.ror era ' ~ 9' ~o~sn o without any wke in
vtr!tohao or spuntIhe?ua1y suSoopt blo ~nim U (Waldmdnn a
Trnutwoin)i i l r rooult~ were obtaitiod tho a grirnrnts of
trt~b, Ue iioQ nd s tho invo bi tb o.
In abtbn ?Us intavtion, as has boon os~tblishsd by a nuub?r
of invQStiators, tha vir qUf beropat?d lonthty thrauh dfy
ano t jp Qf &M l 'NithQUt woakonir off' itu vir i'o wo
Vith prQ1Of od ago o o thu vtrut it vtru o?loo Y bo
waakoned at timen i'or anatho r type oi' aniLsal, but this woakonin
rafnains of little dttratiot, sine tho virtAs vastly adapts itself to
a new type oi' aniE~i and booors infoativa i'or it. Ooneequontly,.
Thr woakonin5 OL' tha vita' viri ciOQ and ~ubsogtont scouring of
the aropdrtie8 tho rn hod of proton ed pa,asa o has not justifiod
itael' and has no prnetion1 stgniloahao.
TEE PLUITY OF TYPES OF THE SOT-AND-1OUT~1-DISEASE VIRUS,
The Frenoh inveati btors Valiee said Darn drat winounoed in
1921 the asmwnption of the existenno of a plurality of viruses. In
1922, ih the oxporiu?ntal' study of the `ii unoio ioal proportion of
root and motitth?disease viruaee of 1}renoh and O?rman origin they
.
d red two iinuw.caio~ieally different types of thQ virus, indi-
iaoovs
oabvd by them with the lettore 0 and A.` Type 0 i$ a foot-andMtaouth-
disoaeo virus of Frenoh origin from the dopartmont of the Oise and
Typ?A.?is of German on to from Eastern Prussia (Allemagne).
~
The essenoo of this noteworthy disoovery is in the fact that
oh of those two typos oause~ imm~tttlty only with reopeot to the
8b-
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
~inii a~ o
aa~ma~s w~~oh ~av~ dovo~ous
r have b~~~~ ~ u~~~o to
otIQ~ wIth vtr t~e 0 ~d
Oou~b oL n
uo of th~~ oar typo Ada b? tn~da?a
*a~~ftt~d iO~ttsa wtth vir
tinw wftri viruo type A. Suoh fQotiQa i~ atoo
ooaond g ~~
? wh?iah havo dovolop+!d ot-+ajid?u th
~i r@ vor~o ordox ? ?~ ? ?
d~~$a~o of t~-Pe A ttnt whloh u r6 iiuiuUflo to U sari `bo ~iioUt?d w.th
typo 0 virus (FEU? ) ?
k
F1~uxo ? ~oot of a aVfar flflflt ?L' ~aA~~and"
3 p~
o suit off` expQ xl~nontal ~niQOt~oa with
mouth diooaoo aaa r
virus teas 0, A and (ale r 'eal~anai~ and
00mo1~te1Y uniform v1tn1aa1
X00 0 ~td A vir'~aoa aauga a
o1otvre ut the disoaa?~ o,nd.tre di stih~~~ shed not 'by their piatha~
nia aotlon an` the o,nirt&1 or~~~nrn, b? by the imp
at of tha o~istena~ ~~ tsature off. various
19~aa1 aro~ortioa~ For oro se
typ4e ?f Md~~o'~e? ~tlrua proot~nt-days tnveeti~atoro u
~'oo~.a~dmouth o
~t1ari a~ot oxa1usiy? ~?ver~11~'1ea ,
tho mothod o1 arose-i t
for the she our~osQS sorola~la~1 reM
therQ are a~bem~ts to apr)1y
~1otions (?OK) by sttaane of prepdratof
t Qa1e~1 and othors) f howevsr, tl~eao
~'~e ~ntl~ens (So1ovye~t~e1m,
no's yet 1ot~ the stake of ie~barabory re'
1~vo saarah
sti~ati0ns ha'~ b
Thandi oxp?rfinentallY the
Whi~e' Va1:Lee and C?~rro wars
exietOnan o1 e. P1ura1i~y of typo o~ viruses, aflotha r ~nve ott~ator
h ` othO8ls of a ot foot-
5ri Franne, Sahe~a, anrt:otiuiaed th Yp
sible
kfeeaes", ae a aonsequean o the pos~
and-mouthwdi~easa s1
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
Tk ?~fWflad? tha axl~taal@ ai ~t 1t' tV 1'Q'
ro
a k` ,"gyp tQuo ~'avQr9 (Div
3CV~~tl~fh4tl~~~~R10i~4i~ M1?Qttt~16?
"pht411 ?'avar" (Fvrahhoid?) wh1oh to
uii
5a1atn' op1rtafl tire
'ti dt~ooVory ot.tha ~1ur~1~
i'aro j1p11o0' ~ ~nd Mrro
gad` vtru$ oar~ttnv~atlotoa
tna
u.n ?irin 1u uflttY" o~
i ou ,o1a tly
~ ~ootMd~mouth~dlooaao (?okah13ha'yi
io1lowto~ dovalo~oi~i~t ot th
Kovauh , qtratol - Rudova i 1tooro, nit atto:'b)
Ui~a?vdrY `dad dalfllta 1UUmthflation of
`Va11aa' o d arro ~
soot-~-~ou~h ~~oo and at tha ~
quQUtlata off' i>~ur~-i1ty to d~~ ~
time- a,ttr1otod the ~t~tantion oL ~tha r to rho duo otior~
the f~1urt r a typos off' vviz"uaoa.
of study o~
Vtia-1dznarL1 a i+1~yor in the R1ema In~tltuto oould .not ootrm
Vo111eo' g and Larj`o` a djoaovario~ on tho bis ol' e~rtr&etttt1 study
rtiou ot y1;rutQ~ of Borman, Frai1oh , and
of the 1mnum1oloioe~1 prop ~
dog
11d, and urbury, ohoaktn the
ltal t1 or1~~~ . hod ion,
.p ro a,1ao raachod nogati~o aot~al~~slarta
oovory ~ad? by V~-~1?e and uar ,
or thQ b~a1 o~ thQ1r oxperlmont~l invo at1t'ons ~ Az-d or1y mush
s
d' '~ rau`~voin (i96) in attitidyln~ ~0 ~tr~-ina o~
n1
1~tor did ~`aldmaun
T confirm the djaaovey of the p1ura11 Y
virof v~rioUs r~r3~1
ao vjru~?r~ redo by Vo11ee end Carro
of Foot?o,nclMrnouth-di~oa
dl~oov?rod ~4i11 'q. third tYpa
Waldrnann and Trautw?1n th burn
of vlxue, which tltoyoall hype e. mug, to reo jotiat
/,~~"a` w
Y Y
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
vat o &nd rr~ on the hond, tho ~orn nvooti tOrs
of tho 4tho2', ooflQ1uivoY o~tobohoa
and
o rrfiQt tho ~xtgtonct~ n t3rQ off`' loot
tl
thr 1 0~ dtoroht typo' o1 tho foob~&~Nmoth?
d&oo vruu
utwota of i d tho thr~o typ i at virtu who)
and ~ ro
Gor A oo r 'o ~pofld( d o Fronoh 0, d
troy ~t~cdid1 A , ~, x1 C
Oo*:n I to Fronoh A).
Tho dioaovory oL the plurality types of vii'uoeo wns after-
ward i aonfirutO o nrirnenta11y by Lebai11y in FrMOO (192.6), by
s
L Oa aitla d, ant 9u rbury (1927) in gland, by Ol ituky, Train,
and Sohaonlna (1926 ) in tho USA, by Mt nuaoon and armanoeon Ln
.
fl the Ar.,ontino (1929), by ?inka1 in }~a11aflc
Swollen, by ~i~~iores
(1924.19?0 ), by PtwararzYk in Poland (925-19 O ), Ind by other in-
ho r1ura1ity at virusa3 was oonfirmed also by numoroua'.
,v45tiLator?
Bono by 8uri in Swit~orland, by JenaQf in
epi~oatolo~ioal observa
Dent Auetrif, by b, Stax~i in 1t t1y, and others. Only
~-rk, by gasper in
agrtner (1927) oottbinuad, on the basis of
Ern, hut, and . ~ opfen ~
their sm~t11 investi,atons, to deny tho plurality of virus-typ?s.
tidied a total of only d atras of virus of
~noidontally~ the;~ ~t
OarSwodish origin, whioh aotuaily oou1d hn~va boon
tt~tl, ~ r?noh, and And finally, , thea? odrimonts wore oar~~iod
+~rus of .. the _ oo,tne type. ~
whioh makes thorn for this retton extremely
out only ot~ ruin?a
uttoonv~in?in5
torrtabionalpizootio 9ureau in Faris, 'on the
1n 1928 tho n
b~ois of 's report "On the Flurelity of Typed of Foot-ar; i'' Vallee ?
~~ introrluoed a resolution to the effeot that
m~+uthMcli~ease Virusos
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
it wdo flow nooooodry b oonoidor orovon tho oxi~tonoo ohroo
imrunolo iva1iy di `for~nt type o1' viz. Id thin oonr~oobion
yin 1@ r ornatlona nomm 1&tnre f?r typoo o the virus woo
1ntrodut typo 0, type A, aid type U.
Vdi1oe acrd uar o, aid a o Y W nn .w1 rautwoin a'rirra
that- the type o of vi ro~o a X1000orod by thorn on~ou fi1]y oontt
irflfflu1o1oiad1 POPor~ta, Va11o end Cuxro otudiod ?or U year's the
type ohpraatri~tiao o strain of typo 0 and A vir&aoa, oxpoln
thornn to tiro rehatian of to moat divorau fdotoro. Thoy props dtod
them in paaRaea through various forme of animaia, stored thorn at
various temper&re~, shared the pM of ho m,d um, froze then,
do i3oatod then, oaueod ohorniodl a ento to reQot with there, and ire
d11 ooUOs thou vtruooe eitor fully kop thoir ori~inai type
oharactorieticp or did.
nid:n&nn and Trautwotn dieoovored anothor whole caries of
atypioa1' strain of viruaea.whieh could not bo o1ieei1'iad ao a
ein&e orr? of the three tyoea diaoovarod eorlior by yahoo and
(:arse, anal Qi so by theineolvea. evidently atypiaQi etruina are
rathor frequently enooutiterda
Thay were discovered aico by V~11e.e dnd ~Qrre in Frar~oo, by
Mannin~or- in Hun cry, and by Ma ruaaort 1n Srndon.
Trautwein, in dif:rorontiting 7~ strains of virunoe rooeiaod
frorft different plaaos, ostablinhod wolf-dofinod type oharactoriotiaa
for only 29 strains (25 strains were o1asifiod as: typo A (0), 3
rtraina ae type (A), and i etrs,in ~s type a, but the romaining 49
trdinc proved to bo atypioal trains (variants ). of thorn, 25 and ne
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0
 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
Moro vario1nto o too A (@ , ~~ vor 'fin ~ t (A)
.n4 18 try na v~ri&nt~ !typo ~. yr ion ~tr~Eh~ woo di~-
gv4irOfd in t~ Burin ? the epioti~ of t tot-Rno-e t c d 6@ ~
p/~
iF H
in ~wit~or?1an4 (r i) d too o yoar i Oor ny (Vr t n).
~
In bri~nt (Viand) 8 otr~i war* i 3ti c oo i ootod o
iit
ro iaii of ~ i~nd' Q thor, 1 otrnina were o aooiLiod as
vrioua ~
typo 0, 22 t a A 2' so typo . T romainin 13 Utz' t prcv i
iual &8c1 were not diffo 'oht d~
%Valdi.nn and Trautvro in o atab1 i ahod loo that upon ~ traina oL
the me typo of vtru~ ao:~otdor~bio dirfrohodo Ord raquotiy obw
s a
narvod. In Va1)oo' a and hinjtrd'a oxpoi'ints individual otrtins of
vi1rusos with vory hi5h viru1onav for opor~tanao~aoly susaoptibie
on?ttlo ird ~-o o uould it bo tr&nsfdrrod to uinou pi s
animat (
th on ouaoo a opt all. In Our ny in 1927 and 1 22, as notod
rt a i ootid of foot and~n $ th disoac was obsorvod whioh
altoady, a p
:
affantod orlY pee `and did dot ?t?oad to unttlo, ovon when the
. ~
latoor wore in a sin 1o buiidin~ with siak hogs. The opiootio
~
amng ho s vas cauood in this ease by toot? d-mouthMdi$naao typo
o ~
4 'virus. ~`aidrr1&n2t suacoac ed oasiiy in infoeting inoa oigs and
hots with this virus, but with great difficulty in irtfeoting cattle.
In the Riorn Institute 4 stroins reaeivod from Ar onbine
ware invoati ated Thoy possosood high viru1enoo for spontanooosly
~
su tibla artiralu1 but only two of thorn wore tr sforrod suooesa-
ac?
filly '~ to iuoa i s Ono cf them ( a atrsin off' virus typo C').
gu p ~
neforred suonensfu1iy to guinoai~ only in thf course of
was. bra
half a your, by moans of alternate po. i gos through o ttie and hop
..
In hie day Li nioros roportou strilar foots ?`rotn Argentina, ovidontly
.. g
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
hRvindt wits tho o iio gtrn o tho vi ru o .
in tonQo invuebi Ito o oboorV@ 1 bha k11Owf oil?ot~-"
In t'trg?
vita of thu viruo Lor ono type o nip ah1 ito woak1y nii'o tad
viru1Qnoo Lod' othur ty Q~ . a 6~
?rooont?1 y s`oion o daa riot,yot vivo t oonueivu anowor to
thu quuution in what manner various iiununo1oioa1 difforonooa ooaur
atnof ditfarent typuo end vartahto of virus. however, it rnay b@
aohaid?rod oebab ioh t1 t repeat?c paosr a o' the as a train o
virus through d?finita types animals ash altar its bio1o ioal
ro ortioa anti Avon alter its typo alaoaifio> tion ( i ioh oorrutt ion
on toot?and-i cuth diseaoo}
Mannt&er `attire thnt tha virus in the or tMam`af anirritle
,~
dSh1y euso?ptib1e to foot and Mouth di easy (oatt1e :and hop
under oes oon~idorablo ohdn~o . In his opinion this is o 1ained
b the are nurnbor of atypioal strains, disoovored in tho Rtcrn
Institute t tho differentiation of virus taken from oattle and
reoeivod1 train various oowitrioe
Vory i.tterestin data on the mutability of the viva in paste
ea ih various typos a3' aoitt 1s was roporbod by the English oom-
mis~aior on footm and-mouth disoaso. Tho virus R. V. 1.6, obtainod
the proao~ts of
from Southern Rhodosia, was olastL'iod as typo 0 in
doerminattt of typo ctharaotOristos. In oro~s-itifootion with one
of those; strine of tho virus gtainea pigs aoquired in all oases a
well-defined irrmi ttiity to. type 0 virus. Cattle in 'eoted with one of
V. 14 virueo a did not aoguire, on the other hand, impunity
the R
0 virtte. Animals which had d?vel?ped foot?dndwrnouth
to hype
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
to ~~' PFJ boo~II1 ?~ak w~bl~
d~~o~-ao t woro ~~wflo,
ITQfl n~otia With one o the R. V ? 14
viru'
t~~utNoifl and RePpin i!1 turn ?to ~ very obtQrva~
u ~~~ ~ oo
~
tiorc, t1tarbor paoo~~o~ ~~ ~ ~ pi % tho&' riflb~
av? ibho proport os of typo Q '(vtu'i t
atp31e~3 straito bo~an to i
~
oL ~a o~ ~ruao~ in
au d'o roni t o 0). thara, i typ
tea otiial~od 4& varinto of hypo A, at whioh
~iomo ~,~ti~uto,
for ~aaoain uiflva pi~~ ao~ulro~
22 ~br~in o thooo viruaa a
All the roportloa typioal or type k
rvdti?n o to "fixaiofl" oD virusoa in paasae
A_ Qimilar obs
~ way iatod >alaa by the lio~
throe tho or~onia? o~ inoa pig
eaigoion* $train oi' virus No 7 a B ~ d#~~ero~tiation aooord?
ator 10 pas3a~?~ irk
ir ~ raaahod type At ~
~ . to thQir propQrtio s a=p
uinoa p 0o to type AbacWn? etill: more sharply dog
~~,~a thia rosamblan
on was also rnado by tha ~ni sh acs~Ls Sian
f`inod? A sjlrar abnervati
In Liao1a3hire iii 193 virus was taken
on jootMaad-mouth disoaso
?rorsfi a siok ax, tho viru have provod, fo11owin~ ari~t11&l di~~?ran~
s
~
ti.tinn, t~ bo a vttria~t of tYpo 0. ? 2ube~qUent1y, in pas~a4e in
ps tht~ strain. oL the. virus aoquirod all proporoies typical
whiah had aarlter dovelopod foot~afld-mouth
ot :.,typo 0 virus . Animals
virus did rat become siok upon artif oiai
rsoase causa~d by hypo r
on with virus of tho some typo.
in~~ati
;Lon ley, and Tarrson, irk de"
' ~vesti~ators
os The 1ii~1ish fool, ~
?inin5 the type alassiPiaation o C Foot-~:dMmouth~disoase virus off'
oattla whio1L had contracted the sioknesC gaturally, investigated
93.
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
tho viroo ai tan~ou~i ` with both o ttlo I ldd pipe n
vireo whioh had been propeat@4 in aattio, it wao
di~~'o~ ontiat~n~
fAUnd that the vtrue had `d dofinite kinship with two type ^? 0
std A. Upon th ?'irat sea o df thin strain in uino~ pio ho
?
s worn ob~orv d upon paoea o' oL it in oattie
aoJr pr?po rti
O l?th sea 0 in 6uinoa pig it aoquired all tho pxo-
but after ~h p ~
0j and aftor tho 14th pa oa o, way di Turontiatad
portiiu oil too .
rvoni th pa~oau the ant~ento vroportio~ of
typo C. In rho into
all throo` typao
4 A, and C - wore diooovproci
In &tandd in 1~3 considerably morn train of type A wore
dif 'orantiated by oomparison with tho romainin types of viruauS-
In individual atrains of tYpu 0 and C v&ruaeo oartain proporttee
with raapoot to typo=iuno1oioai proparttOe wero obsorvod. Tho
n on Foot.and-mouth Oiooase in ono of its rport
~glish Carotis~io
whan of 470 hose of oatt1e itnnun? to all thrau type8
oitos a oase
of the virus .? C, A, C 44 hoad of stook bocdm4 siok upon irifeo'
urth time with a now stz'ain `of the virus ? 10erma y
Lion or the fo
in 18O there was reoordod a eooond dove1opr1t oL foot?u.smouth
disease by stank in the oaxn horde, oausod by 'vireo whioh upon
..:. -
differentiaoion h4 in both oasos.been a1aesifed as th?'same type.
It 1 diffiouit toexplain tie sioknose of stook in this inctanoo
8
by a "falling off" of iuntty rosu1tin from ropoatod oontraotton
o, It is most probable that the fourth time the
of the _dienas
with virus far romovod in its immunological
stook were infpated
ro ertieo from typo o, A, and C virus
Wal&nann ms that `aYpt ioal strains are enoountered all the
affir
moro often as ; the tendenov to wide spread of an epi~ootio beootnee
more
r trdngly i nifeste4~
"I-
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
Durt thQ??rj t111 fl
ualaaa~l humbaZ' o~ pa~t~t~tta~ paiflt~
Oarffly 1926, whvu ~
(i ?,OQO r~rm tvod6 tt~ ka by di~ado~) wra tf'?
M d?mvthhruo types o' t~-a viruQ war?
~'~at~d by ~oot
0 A, d , dhd to?ir ~~ri~nty~ ~a nnin ?n
di~i'a rentiatvd s ,
~radualty
lp2~f v~hvn epizootio droon~6 tarta ?~' ~?o
type Od ito vor i to oou1d to di fern
to $ubcide, only
tiatad vuoavocfut1f.
W~1d i, ' o d t tv yedr+ vuhor oa ?dld-
Aooardin ~ to
~v htt n sharply da tinod tendon t to rapid ?pizootio
moutli dtr~aa
vprv hrao tYp o -- 0, A, aid -~ are Onoountorvd, and in
ad all ~
yvLrs ot "oalm' only the o in 1e type C and its vta' arto
r~iativv
,ara enoo~~turvd~ On tic ciut wail t t i tpt d to tine t duaa .
vtrl~noa oL now typos and varitnto drin~
aan~xouttafl botwothe ~p~
api~oatta~ r~d th vuvv ritY a the oorvo of ho opt.
d
>e ~~art~ot~r ~n
dootiat thenne1vaa
nvovtt ;ators oharo Watdn1rl11't paint?afMview iii thto
Nat an t ~
rtioulur, KitdytdCOVj t~rtn t -e oot~ nd? aut api-
t
oottcn in Central oboextvod a reverse phonornOnant in the
~aat?andw~outh-dia~dee
years (i~ $ 1p4?) with uma11e~;t Aproad ai' the
Oted vartantU of typao 0 ld' A, O d in thp.
epi~oatic he di~~ar?nti
teat inoroaav at' tho opctic wave he
years (1941-1942) of area
'e rontttted ' oiy one variant o typo 0.
dig
ermaoY, end a1eo Ririiard in Pranoe, ix en ox
~aldmann in
t~and?n~0uth^did@~QQ ?pi~ooti0 Af ,
pertmentSl irlvnoti atio i of the boo
onuhtv of thftt the vtrue in co~ditia a of'
i93'?-3 reached the o
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n~tr dvo~p of thopiato o~n. ohafa ito an~~hc
the infittohoo of & ov r~i aro t & nembor of
otruat1ro Undor
o to QQt-1nd?1MJ1th dig goof fdvin ohgn~od ito
a art~oo it b~~a~o a~to~r-rrard~ ~aW1~
ntifonio
pv@rQ0U sufity tn Rio, ~oquirod by thoii tro-
the ?zijirLi tYa o ~ virUa . Thy ch oticn @' tho o t i Oc and eo d =
,
oaqurrenda of vaFioI1 type d variant of to 'rir
t~0fl6 0f the
d?v?1amcnt of apioctioo ooatinuo~ to attract tho
iri th@ poriod cf
attention at pro?nt^~1ay invo~tiatoi$* Moanvthil@, soienao ivoo
ua na oonol aivo Ji Wor to thi&~ uoot1ou1, Which hao goat thaoro-
tioai acd praotioal ~i nifionoov'
Tho n pooranoe of atypicai ruo !o Div most oaaiiy bo oboo'vod
Lt tho vory bo i anin of n o~ tbrouk of app ootio. Man inr oxolaino
f ~
~ id pace=o cf tho virus in anitrio highly
~'
thio phQnas~~non by the r?p
~enoi4,ivu to foot-ltiouth diooa~Q -- 4?btlo, hobo, she?p, eto
t thu atypioui ctrain~ ao '~~,o~itionai f'arI" --
~e~~itd e
~ rautv~ei~
v~U Antt I In tRio tutor' o o jtt%,on, thoy r'opx'o ort t othi11 mot' th
i p
'big a re~oion of tho phyeiolo ioel adaptation of tho foot and-month
diooao(i viruo to t dotorrtUato tedium"f Tho li h (onun coictr on
th Dinoonaid rf tho fallow n tho most accurate
poot and rnou
xf~ianation of tho on in o atypicci etrainc -- viruuoo with
tliff~~ cat tIJfl otrain~ of foot-
~c-u iQ cai prapdnoitio~ c
nd mouthtype... ~
-diMeade vdruec oonci~t of the namo irnrunaloical corn-
pon~n1te, but tho quantity of the latter oan vary. A striotly de-
of thoao ini uno1ACiaal oompon?nte is expressed only
fitied quantity
in the etai~dard typos 0, A, and: C". This oorrmieui?n as.u
~.ru~ee of th
eumeti that in o onchtime of natur l developnont of ~, foat-ancl"mouth
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
o~io i~otio with 1fx~d Vrua two drat'
i they, iate' pa~~aCa, ono o~
p pobie. ub~ote~t `
th? t (!-qt~fl eos~!
~a' ) the otho~?. T~'UtYt@i1thorn, a~ ~ ~ ~~ ~~'0~"Id~
tQd nirea pi with mixed vir~avo o
ax~or~ntai aortditions, in~'oa
evai type, o -the oz t~e 0 r~z!>~i~-~ aiw~y~ rt~~e~
~r6 y
nd the othor tea timir~ly oro~rd~d opt
punt; in Ulf a pi,
by tho l aster.
In tha at,isi n of n li investi n ?' ae a, Lan ivy, c1
Tamao~4, rtain tt pea of vir~ues hay=a a vary as=tp1OX ii~oa1oio~1
th oor~diti?na of r~tural dovelop~nt of epizaatiesahoy
otrueture
thshy vir~u4 the ar an':s~~ of tho Linea pig can alter i:ts
a s a ~tme at t
a nea' 'l=x~~?~atiana~`
ti a is atru turli and di sooiat , ono, dr a rose~t, w
in vnri~ of tra sr isston of :the vir~rEs in the
oi` ,~tt sins
prooe" off' ita na~sa w n ooour. and ~aamla oao~dedp'
ai' .Eavcu?ian tyke A vino; and Milan typo C
in the r)raoo~a of puscare
virus yitiea iii ooiivortt; thorn Into type 0 t 'tor tho th
in pigs,
pasr~ae
aaot'din . ? o data cf tho came invostira~ors, the Riers viruses
of ti nd U, of or prolongo i store o if a lyaeriXe pr'eservt tivU
es 1k a
aoqwirad the prapertits of
nodiwn &rnd without to in ulna pip,
e'ible niter this to oonvort these strains
te U, it tee no loner po
beak rota type A nd C. r~ iartnin 'or of f Irmo that virueea of type A and
Tho latter is the most oonotant typo of
oat ~d over info tyke 0
VtrusoS of typos A ana O, aooor(Uf to
i'oot~and-mouth?diser34se virus
are observed only at tho bdinnif of
:the q,>athor' ~ dada, as a rul
ro newty tranafarmod into type 0 The
?pi~ootic:, and at its enc~ a
97
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
to ~fl
o~rt%es end dooa not ro ?
into ahUr tyo~
IdontaiJ-y,- in Tr~UtVio~rr~ o p
~h~~h oan ~o ~hbo~~r
the pho~~oa;~n~ at ~itt~i~~to* ~
" f@ Ito LLot WI'. I O O i t~Zao
o
~`~ .
o ro the propertiea oL typo 0
atra~n f~~ ~~ A ~~r~#r~ t~~~ao q
~tiotr ~i~atono~ o ~tra o~
ao a a ui 4 u loot its ?nmun-
tyto C vtruo , aftol ~
third
ii~~ ~roperti~ with cut to itio1t. And ?in~13Y, c'
vtr t o' the "M 7'1'i" atr to flrAt oa~tood
oaoo the l~ot?~nd-~o~th
~~~-d oar~~ er doveio~d roo r~ron~
otokne~o iz anintt1' w
o~ =o1~owoct tho. raartioc of a ptza
oodad to obMorvt &n iftanoe whop Rio
~trn~n. $korokhav coo
a tc r a Yo r~adp~-M~ia ' of pa rda e
s~and~rc~-tY~o v&rtt~oo A on~l ~ ~
~1, 1o~t
1 ~ ' .1 aar~r~o prt~ve r~dr~
rid ot~ra~ze
in ~ir
thetr ari~iria1 tYeMt?r~-Portto~ svox'e trfo~'td into o+ypiodl
stra~no vaiti~ the prraa~rtio~ Y.
onerti are not striotlY aata~t tYpoa
~d_moth ~dlsoaaa vir~t~e~ ~ to
sonGt, but ` at~c ar-1y di1t'o rout kind -? var~a~
in tho ~~nc~~o~lca~
off` virus type 0.
ataU1y
wtth PO ~ A -end C, ati
To a virta~, .by oom~ar34rgon
rp while
_ o aesues thg aharp1Y eL'ined conotant-fie proporiea,
e maot ~
o 0 under oandi-
eo aan bo tranaformed into kyp
Mthe ~ono .~~ wh3.Aoh and ~ have nGod rua
of boen deternineds J. Lt niere8 of Amen
y d
~ `t~a~ar~ etr~ot~Y deter-~ne
aoidex that in do notexi~t
senora
' ex1et only varlantB o~ the aJflO
t es of the virti-a, but that there
98
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
ntig a t tto r two-ye ae d o i pt e i
io
Obrv~t4n~ Q r no 1e~crr ihtorot were cede the Ens
t-aftd??iiouthiceae~ v~ith repeat to the pnecibo
~o~fl:gcoion Qn ?oQ
saeiation o e 'OOt* d?r o~~th~dieeace v rub. 1f
Hal t th
c~itti~~
inkir t . Qno f tie I trtino with atyp is&i
tho proaece at
train No 12) a rood otab o until the 20th ee o,
prapertioa (e
a " ctrtinc, . of which ox oacecaed
and thou I 1~t" into P . two "aubeidirY
~
all oharae tertie4 it typo 4, the other the nroportaa of
torictio bra. .
type A. ?or c iittin of this etrain the authors ucod the ?oilowin~
thod Two ~roup ff' wove infected with tho vir~tc, of
inoa i ,c
wht oh one reur w n i -unizod tc typo Q end the othrr to typo 1A ?
of t} virtt the A viruo wad ~tistin~uiahed in tho
ditierontiation
roup c a i ttni e i to typo , and the C 'lets wt dic-
e~ a i=gea .. i~
tin~uiahoc in the athor group, is tW1 1~ed t ty) A.
In tha liht of tho data the very quoction of tho a:istonoa
~
in nature hrec rciativaly oorictant types of foot"andymouth-
Of only t
se virus (0, A, and 4) sir4 off` at'picai ctraine, vs-riantc of
dices
theca stUt types, is irL Como doubt.
To nlieholmission ors Foot~and?mOUth Disease considers
that in great Sritat the quantity of typo c and var1ant c at vi ruse o
is 00iderab1y r tor and that they are more divercifiod than he
had. In its opi iioo., to ether with types of the
so far been establ.is
virtu; which cu 'e gtab1o in the imrmolo coal c? o thane are also
If tiona):, ?ormc which could be oe11ed unstable
, or transi
_"interrl~diate
type iowover, the majorityof strains atiil does not depart tram
tho limits of the standdrd trp ec D, A, act C which are 1C10wn to uc.
-
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
a~r~tac a d~ Qa with tra '~aaar i lfl Qr! I
tt he h~~ rY^
ink th~ trnttQfl o~ 1p6 af viF tflt~ o~r wta ma~@
' Vot.ri~t'Y uo~~~ao ~~- -tofl ~~- ~~~~,
fiat the Itarr?~tian~
ntoz ,~tio di f i~Oatia 4'W f
end not &t ~ a~oaion of tfla
3ia i*
~~nin~~~' ~ 44t~~- a~rooaa~
1`a1d~tu, i ~ aonrL@utic,n wit A
at too ofl~r~1 fl London t w idea that it ta` hooro-
a
~ator~n~r~- ~a~ ~
to the rttutabiiitY t' tYPo~ t~~ t~~o vi~~a
tia~iiy iooaibi o to ro
t1 this t4 1't LUt th9 bii#tioo o i'orti-
t\nd a~~n~ooti?u ti i
iQ~i o~ row or oL' bYra tynos~ durin ovon ona
ap ?ootio, but tht t opiniot~, too a ,
~ti 11 pryh'At tro .
t3ana~or~~tic+n ' c~~~o
ao~a off ` tho a~
~~i~t sor vpori toittal rat'
~ ~oodo~ a Vat ioa att4 t~rro o~:oo to
.~~~~, o ~ vi~~ tts into ~~~o toor art' ~
t~ off' vii which ~i4~ ct ji~4iiit~-~d too
not doP~- ~ m~~ta~bia,i~~~ of Y~
~orc ti ~t~ of ono typo iota tnothot'
The 4t~r; ~ti ott o t the ~~&4tY o~ vi#ros typo~ wao ono fit' the
4orntrr11 'roblo~o a,t the 11th It tit Vot~trirfl1ry Uon~rOdn in
' d Li~nferU an
- ~d , ~{aidmaun, o
of an
rewos
,dan, whore too roports
im
prob'ioms foot th diseaaO w0ro pre~e~tod. In its 4on-
o~ ?andMmou ,,
olttr~ior~s the Con~roe~ aokn?W14d 4d that th4 4XiBton44 41 a
~
oi' nif~.oancQ for ao4r~rato, tiu~d~ratand~.r~~
'~Y of viruoo~hay tramendou ~
I ? .
~~~dMmou~h' dtsea~o, and ai~o for soiut on
_dr tea Qpizoobolo- o~~~~oo
of the p~oblom of aotive ~rtd paaeivg imr~nixatian? the Congro~o
aSr,nowiedged it dgsirabio tbtLt in all 4ottntrio~ oi tho world
~ ae
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~~ ~ . n ~ ~
- 0
 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
of q 6 Q ?
;*uiouth 4'
Lt ? tW YtW'Y
{r~.t1~}ffQ (~~tmkiv "1t".",~o V~~ao?~}
.. ~pq fi. i1:k trll Yf:klC ill d ... ;.1 ...~
v a~ui~ era fit~tt V otztr
~w~th trW rrp0 ~~ortv~ tt
i~ w~~~ to
of th 1trttd vir~W tvtO Y
~t totko
- t4
Atl+w~
ro be ?v
1'o, thotrt~tY
t~~~t), and ~,ria (p
nUao1-), SwttZot1
swoaon ~
fa off' dY y~
on the baot
it to or Dome
them `rom v riou~ oo~ trt"
reoejged by off' ~~~~e for
of thevc~nc~ c i~rihu~ior~
oor,oepion
tr~~
riGBa The 172 strtiU of he
0otte b? horn re o. mom
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
Th ifl tYp6 ?; ra oo .r Q qtr
at
~~ ~~ Ai ~IitHEdd~t7s*R rM V H~klA~txtl `a+~ 1P
~N t ti tt~t1
type A ~d etr~t of typ !Y1 f~ilkH~ AM
~'u@a~dvd .. ~ ~tra~n at ~y o~ ~yp@ 4 ~ ~u~da~ ?" ~
~ ~train~ 0.t ? Q t1fl arY -- 2 tr
n
t Q d 3 ~tratn a too 0 j tmrk -. 2 ptraln~ or
~t~~~1~ta a~'
a~ ~,~ ~a a ~r~ont~~ . ~
n~ y
t a Oj ~alland .. 2 ~trai t
o ~ ? ~'r?m thla ciat~ it t obvioua
or type 0 and 3 ~tralnn a~ tYp
10~ typo 0 (82 pornont),
. o rodo~~lfl11t typo tn all aotr
th?~t p
w oilY ~ a'!~o~ ~~~ all (~ pay
1- t ?~~,~ori dc~ovored 1n ~~l0 Yp
aarb ), aid tyt~ o
auntrl~a X12 p(uraont)o~urallty ty~oc the
th~i ~~~ too q~~o~tlon o~ ~ W
td 1taorapl~aa1 dlatributlo on
~'o@~^ll~~~"m4uth?d~a@~D@ 'Vi1"t~0
n ~~.a161o~ato t~~d;t~ Tho
itor~ o tho aoul` 17 . o bon i~
the torn -~ at tho t~mQ off`
t'irat a.~ompt8 to otwdy it dtito from 1927, whop ovo ,
tie 1i the U~:ralna, ?1r~t di~"o~?on-
teatrod two d9~jitho ~?ot?d.mputh~d~aoas~ epl~oo , o
61oX1ent tYpo0 of the ~riru~1 ith th
irc,~m~ola~~ca11~ ~ a
d of oro . menu d ton 1n' i inaa pi, 9 trait ed to rOmb
g type 0, ar-d the tonth strain prov
wore alassl~`1?d Vad in 126,
I1nid,e~tt&lly, SoTolvyUlV obBI r
the co~rcfl~n typo (4~")`
rm~, absar'od a thriaa
ft
on ?no or ~h~ loadln~ had a hg ~
Dot.. d?mouth' dUQaeo of the Demo
ropUVt9d sicko~ln~ ~rtlth ~
a tho enures off' aye y
over
ritar ~wmmor, ~d 1ct~ i1)
~ w3~ - with
riot ~t eannoct thla ph?nomeno~
k - = n that tiro tho author d A gY
ty~ea the v~ru~~
the p1ra1itY ?~ o~
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
Q t n ~ Q th* n ~ rci 1 c .P the vi r1 hav ~ a
On the i lafd
f or1~1y(Jy{[J1n(i4 t 3ei i V' ifl 1? ~tr~tIIt f I1
w i c t d , r o i' d ~; d1' ?t r ~t tin a t?reia the port t< rfl
t
~~ nt~t~
end w~r
the
i; 'f the Union. Ail ` hem w r a1 ~i oct, on the
~htr di ttri
trtnvr! i uni Mior~ in ~ pint, ty a 0, a1 in
At thnt i;ict thi norv d a bani A r as alu ion that
f'A ` h?f tii ite bctr= tnfr w r Q nti `i pr(' t t ft i v r41 ty
vir t v; r d n` tr~i E~~t d n the territ ry tho tJni n, ~ "it
I n y to tjrdUU~o ( thr~t ? +at- -I1 ~ h- t X i o tip a odu~cd not
t ho ;t typo ( a w~ hrt'o tndiuatQct ), try the r
tyt~ hnd v~r'iant'
Ili U140 a to atr S ;2 c:$tkitl r no~art; od tht~t ire oruparativt
i r eir w ~trr n oi` vir'uu tI, ri it t it its two 1 ioaiit? t , Choy
had df.'i`ront,iaod thnu ran~~ i-~rioloid~~ily dii'1'event typen.
They tuti d tyir~o dit`i~orefoe i' tho~+ trainn in uinet pi a by
rann of tho tt otht t o oro n?ir~~A uni ntiori, an 1 t.1 JO b' zr~ariu o study
of th protectiVo pm r io of a arumn of anirr~in vihioh had doveiopod
e '~
data abtained its the oxporiment wore alno' oonfirmod
tho dsaaso. rho
in exile t irr~ nt on enima1o naturally cc~nooptibie to`oot-OInc1MTftouth
M t1e and ho. ttior and Sirotkina 'ddnnider that it
~
dineane? aat
in p roved beyond quention that the two straino of Viruu ntudied by
out to be irr nu oio~ ea11y di 'ferout. nfortuna oly,
than turned
train wan differentiated by thorn with reupoot to type
neither
?lanaifiaation, tnd the biolog.ioal proportion of theno virinon in
;
'3I
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
rtg wQr :
w SAS
T`r
4
v"ia:t:It f` r
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pt
tht
ri
; i1t1 t?A 4
rt: t t"LY
11. , rf ~~a1t V'9~
~tllt~. i-tyy 4i 1Ykbilwk~ty ~...~ttJckiCd~ ~ $3
Ohl~F1 t
4- kroc
pit)
otr y pert of the oxporimontrl
y
and n a,r '?' off` S oertaifl i;;re.
obtrn~flod a~
Kind ekov's study o 22 stro.no,
Ao B1 reou~t oL Y.
py q times o the year i the oour ofyoars, he
Vr~ou Grp a
vii: rt t~ t .1 : t k t
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;t? fl ha ~~ a~- ra~aatad~y
~a 0 ?v1r~a d v~r~
v~r~i a P a A. Tha d t' ubta fad pQr ttad`
I~1ndy~1av to trVi tha aa~ai~aion fat ~n tha Wrr~tarY
tyVaa a~ tha
Aata ha h~d,aat~b~~~had
ru@" (type ~ end tt~ v~ritnta and
do the 1boo~arinta~ data of
Q' type A). n ?gUQft ya
dr e,nd .$js'otktfl~, 1cjn1yakav), ct t1do aL
~ovtit jnvsoti~nta~a (
0 aoto~anal aba~rVttivna (~tvai''Y: aV d atha ro ) it tty b oeh~
a tvrrttarY of our Ufiaf tore my ppo~r
a~dorad pravad that in th
nt tha t~ t4 ?pt~oat~aj tot' with too ohoou~tai'dd
n Y typa o~ vrus nd, ir
d~str~buttod a ~h4 e
type 0, oor, 1e ao bradi
ukiOU1nr, vritnta Qt typos 0 end A.
d the ~h~-d of ox"
~'a~;oo end ` ~arre uoa
il typos of viruua~, oatt1~e sorvt
oiuc~voly for d~~`~~oront~ation o
" thoir xporic11nt~iriig1s . Tho aanaidor thnt ootti~ pro the
t on oL v Ica , n1tho' h use of' them
boat sub~ooto for diVLoron
root du'riau1ti~s ao ooctpnrod with
?nr this EaAurposd irirvo1voa
d oa.r17ifl~ atit~ d~!'~cront~a~~on of ~irua
rcnaa pia. They rooomion
ovvorod from primniy ~n~eot~en, and not in
iti1 ~ni11tEt1~ ivhIoh have ro
tich1dh irnvo boon troatod oxn rjntOiLy ?or th o purpose
tto of tho t oy of vtr%a n whole group of
In tostirr~ imnwn1ty to o
tod with serum of tho toot anii&, U
ping, drat ooia
taken jor exporimont
,~` `ypOs of v~.rv.eaa by t~ooina o~
The method of d~~~e rent~.at~~~
' s wee i'ir ~t worked opt by Voldman~-
crnsa-itnmran~~a`~~ono~ ain6~ p ~~
orfeotod by Wa1dmann and. Trautwo~n
,rtd rainy?r (i924) Qnd than p
(12G). ~omowhat ior it was chewed anrt oorii'irmad by Stoakmorrn
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
. t$ ~d b t~o P41 atv~y
0~ ~OQ'~?~'~0~~~ t~~~~u~~ ~ y n: othOrYO~OOrr
~t? Ot' t0' v~ruTr~utWO~-fl
I~ atud'ifl~ rho plor~
t't uooorE~~i~a~od
uaod tho cl thod off' Dross~m
witbhott with a&tt o f hop.
~t ~
sin aomister tho iinod to mph u~ei ox-
~~and T~ Fuu~~
;uidcnu t
oot.d~Uth djatueo nd
pvrimont~~ i'ot tt~d:r
iy far di(i'oretttiu'tlo~ oL' typ viAso o ~ the inou pi~ da?a riot
at?~id-'ou~h dirrou~o
booemo in oatod with L a
lion stuooeda ruthor euai1y under ox orimQTtui
tovffl, but bhic infoo
oonditioYa?
for diriteror-ttt Lion of etuh ctrai ra tbwoira rooo~t~-onda
f? 6 hou thud
WUir& ho than six rout
t't~noa oboathOd ini tho iabn~uto dram
1iflQU pi~c in u11. All vi
vein od
OLabtie whioli huvo do
ui tL pis for no loco shah 6 to
a,tut be pur,~od ii~ aroiiminurY ~
oalieo c
10 tiitoa. 'Cht.c aooar1ic1~e tr icnu uion" oL rh ~rit' to bha
c a
oz$~;pt1a;ct of the ii ?D i w ?u4 oottco 1W fltiY fltnX" ooitcbunb roanitc
~
rod tt~ot ini`Gotiort' of vit'uoo~
viii b~ abkti
~ z d with reapuub to 0, A~ e,nd J typo rr~ot bo
~uinen ~9,s imm
o ii~tbio f TO bhio end bhoY ua'o prepurect be ovohtU i by meunr of
nd uo r roo't' t'y , i. . o ? in 20 to ~O
t~fl1to(3tion wi'~h bypod virus?o, u
bhore ~-
?if~nb ? I er~oh group ua~ be
~lays, ~~c~y urn used for g p
ar ih ?~kt~er words 2 uitteu for ouoh
no ieos ~h~~ 6 ~u~.tSeu pip,
guinea p imrtttzie to ~yP, od virucUs e,r? foc~ed in this Dose
~a sb, ~c
it. .unknow virus whioh ha boon proptod beorehar-d in guinea ~
wn
pma+ ' uieo be oe,rriad out .&n ~1~inMa
~a
~,~ ~i~`~erun~i~b~,on of ~ ~` -
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
o b Q the re rtUo cr r mi i t1i tUflt41
with 1i tyPM vir4 bUb ?Ur th wh 1 ~
- . th 1i ono Oi &fl t T J fu~t bo ~sa~ ?
ovoiopo~ foot ~~~~
Thy ~x rimntai ui R ~ mint bo ObooVVQEi ,f I1
'r no than to 10 thi,Y, 1 t ro~uita ' obo rvatiof ~r
ioration iiy If pr tio1'V i the aprro Mato
ta~o~t into
In of itucwio i'rori uinoa pica wriah had oon~
ut~voi - stabii h, t i;rt~ o4i ioi y of im;11148Q
tr'i ~ioouso Tr~
bouioo in tho blood of thot ' aoincio. It cio~rf opot the tho oru
ui11oa i~ tr4to With Vit'?@ o ono typo i~opsooo i1Auniif
prop. rjti~ o only u, it that typo o vir .
uona~qtlaxttu, Ffi its tO soy}ur~i of t iVoa p:ts ihi'ooto I s'rith type
A too u~ 'i4 tun bo los c tui t rovoi, i.h oC typo
o:~~y ~~on~~:oo
o io or o f4ot;ivo a ?rit st rho other two typoo
Suc3h soruirti ~rovot ~
sip of the r Q speoi aity- of soru oL uinm
A rnl C. On rho bt
? `oot- ci?mo ~t t1ioot , whioh typo
pigs whioh hrnvo oovoiop
pooitoitY is wo1'1 oafinod for thou, it is poasiblo to ocoom~-iieh
s
dirLol{-~ntiation o . virus with tho hoip oct Costs of tho tonovtiorit
.
otion of r orttr.
Mowovor, thin striot ~or'ovaio~~ao o irnt o 38r1 ha,e boon
ostabiio'ho only fof the ui top pij, whiio in other tnimaio it has
a ~
o devi, tono. In TrautWoin's Q ~rjLfts hoto1?OSefo1o
Doris i~or~ ~i
wgro obaorved in the i1 pinto sorutt of othor aninwis;
r
i ont off' os~s, .f'or pigs in G poroont. Simiir
~'or ~~ttl~o in 6~ ~or~
a -,
o tok~]hocl by rho author with rotpout to the oorux
honornona wore s
p
oonv~,ia~oonto for thou typos of
or r
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
trt~on P ?
0~~~1o ttbo ~d1~tkJWO4t 1
t10?t 3f it UQtn th thQ '
~~~~ a- ~~ ~i~a Q~~~~
~ ~ ~ ~o0~~~o1t~ o~
? ono ht Fop
r~ao~io~ o~ co~~loat~~b?
~~o ~o~ to ~ ~
QflQQ~
,ca4~ tho
thoY o 44u0
to r' Othor
ru?tOflI Tr' a~ Soho O ~-
i coo tvjot~'d the
acot , ar , oat attitu "
mots c1i ' o v'truo.
for
t ~ ~, Boa.
1 zero -tta lot r ? qtr Ypobta
SoirLtyev ad th r uotlar type
for purpo p
ti.;ene prred
red or tho b3~ o~ r~thQr
the author' ~a~a ob~a1
r~~~o~ off
? o#' v? rio n1 1~}, the r~~n
orcent c~nub`~1 shown~o
11 oty O. ,.W 1.2 p
t1nat1o~ ~
a, f-I?ly'~ u~?'~r?4{~~k'~~"~~, {y~kr?i.
PASSA op ' M1 VIRUS ITT 4UI A Pigs
bordto~ oonditons the ?oot?and"mouth diso.Ae virus
~n~t?r lsa
~ itainod in uihaa Pi. virus?e obtlIinod From strut
usual ~.' ~i ~
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
~r ~ i 1d tiro t4' ~ N' i
o to rho typo o ~ni troni w ~h
tho v1ru
toriul hM boon t on dur1l to uro1 oar 1op wb L' the
io and i10o the typo1bo'ia~t~~` t vtru~, ro
opioot1
t
ovidvntly QV no b~an~i1 aii'iaunao~ Suoh a oanoiuaion wua
ro~ohQd b K ndy~kov 9n tho hid u1
g 1t oi' t~~d o v ri u dl tributod ire t1w t rrir~p vi! ontrg1
y
It is inhorent1y obvio~*e that not s,U. viruaea from i 14
haturlliy auadeptib1e tta~~t~ -Tth 4iaoae an b unior~ry
pathos ania for uinoa pigs, insoi't r ao virulonao is not (Y ctriatly
porrnlont and inutabia proporbv of virunos, but on the aontrury U
aubjdat ?~v ognaiderble varintin Thin obviously xpiuio the 1Aao
o' viru1Qnt trooortios u indivit'a1 viruaoa in tho reoois of `pasts
o in in? of T1fl4 t.iohr gtia r ia~ oetion of Guinan t c
ata in tha ?oiiowin ~ Thv virus' pitha1iuln io i'irst of nl1
aonsi
areo].tin marbar with a in i1 quantity o aond'or row el
~rouiid i~ a p
s is d1i1utod with aphyslo1OLia&1 solution i` sodium ohioride,
for this is tr'cu rurjod to tho skin, saariried ih k)relimin~ary,
oL the plantar surra?o (t1) o the roar ox~ro nity of a ~uinoa pis
1`i ure 4) ? 1nrtad of aonririQStian tho "tnnelin" mothod o be
usori. A time/ i rude with the help o1 u syrin a noodle.
u e 4. The feot?and~rcu-di?~sa proouss in the guin?n trig o
the 1m r ur?a-ae off` the roar octromity ( for Waldmnnn and
Trsutwoin.) In teud o this it is possible to pri3k with the some
the surface of the plants of the e:ctremity with simultaneous
needle
nsr'ereno to this pie of ran irjeotion of the virus
trs
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
t t gat R-RVtar too
rotor to rho uo~
Wrth
f vtru~ "boa u~tafl= '" r~tht?
tF~lol~f
v~'tan ~ua~ a
iaM a~ tote i. Enj1r1
ti1Uty ant i?f~tht1y to the or ? n
roaatIn ~uQh p~aa~ thv whivh hvo &Lra~4y
~~o te~a
been aon~ tizod . For this puroe 1a irjrata Oi GubtaRg
ari~o~teaiiy for 2 wka boi'ara tn~oatian'
a~ horuai aatti~ o~rum iatra~
ea ~~ ~a it it r~ao~~~ended that far
For prima'y paooo,geo in ruin
l;oao thafl oix ~uiflv~ be uaad+ sinaa
oaa~' test of the vtrus na
often more than 2340 ,rant dovolop tho disease (TrautWain).
no ~
Yiell-d~~'inQ~
ori~ It a
It is very Odom that n ~ n7 paa
?n ' tho "site oi' ih ~a?tion ~~ too vtruc
de~olopnt of tho infoC~ian
e~
enoraliZttttaare obtatOd at anao with u ea pip.
and. a objvqucnt ~
PL ,e or viruo rra;tt oth r_tyPeo f f ar,i>tals ?which huv
s tho rt~ult a~ n~bira1 in~`ooiion
devalopQ~t ~aot.and?mouth dioeaoe a
spot tcio ao,rriad out 1O 1?os than G to 10 tiit1o, unties deVUlon~aont
fl tho oourso aL 1O-?24 hours acid ,~onorali~atian
of rry 1phthao i
of tho ifl"octi an i tho rollawth~ ,2~-haurc arc o~ooess~ully
~
.
hievad~ In where viru s from cattle..., cannot 'be '~ranef ?rrod
~-c c~oeo _
sucoeOuxiy to.tho uinoa
tai it must bo uoed to ~ L' eat owlno at7'a
thou takon from the awino for fection of uinoa pip. In auoh o,
d~s~ nso`
aaso virus frocrt catt~~o co-n a1mast alw, bo trasLor1ted sucooll~
c, oL altorna~e p~ssa~o. Obaervatiori of
to ruin?a p8 with tho f~i
flea i s must bo carriod out for 8 to 10 daps with
~araulatod dui p ~
of the animal end aorroopandin~
atdly r~~istratio~i of the aonditior~
reaordin~ with r~snect to to oiinioai e,epect o the disease
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
I1Ar4TT R
Tl U ID UTY OF ANiF AL DI A9
FvotM Ui fll th diao~oe ~ttt k@ all ty ar _= aiovo -'aotod
im,1, Undar oonditiono of n~tr~ n~Aton oattl?, awne
sharp, and onto era Hoot oucoibo to ~'ao?-rvth diaao,
aid bufta1o, ai o1, d ruindaur tro aounovinat 1a6o ouoooptib a.
MnonL other torn do nima1~, foot-and-mouth di~oasa on infeot
doffs d seta. Yfith rea~~?et'to the a uoeoptibilitya font-aid-
rrto th disease of horses, the opinions of iit va~ti ators, ~.~d a ao
of t raotioa1 workers, era diver ent. Aunt cnima1s kopt in
ar ptivity (zoological oar', Gardena, etc., ), iraf`au, ilar~ae,
ontolopos, bison, obu, y k , ohano a, roo-book , hi td, rot
land o1ephaAt8 are' 8u3o4?tib1e to tQat- d- i th uiaeaaa.
v~iid ciovon-footed o1xiriai s -in natural aon4itions evidaly
develop foot-and-mouth di st u o very rarely, and havo hardly t.ny do
Vroe of opi2ooto1oioa1 imaortanoe (i!deimn). An excootian to
this general rule, as we sha11 sec further on, is the antelope.
.During foot.send-mouth-diseuso eoi ootics in Mon olia reaostat
aaoos of naturn1;infoetion of an~oiooos (''d ") with foot-and-
mouth disease have .ben observed. ~;odoe of -foot-end-mouth dtoocoo
~ ~ privity era very se'idam encountered,
anion fur-boarin boasts not in ad
aoeordin to Stroh' data, and othor investigators affirm that in
general Choy do not develop Foot-and-mouth disease, oven whoa they
are in ooritarnin1atod 1ooa1ities. Iri the. opinion of oorta3n
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
h~V~ Yh
opiootoio oot?nd?o~obt~ioooo (OITt gni 8tro6),
`owl `try uonidozod un~uocotiblo to toot =end=mouth di~o~oo
and nre not d i'~~t d by t i at '&i oondit n a tit tt ht beoi
porn t1a ~i~in nrtiL oi~i t thodc ?n the lib rotory to i taot,
o nm lo, dory tic hw ku
Amara r?don, cum ooc'u1 trtitioin1 intaotiono with ?oot-
nnd-mouth di~~oo~o havo bon ~ e?moliahod with rctrn t~ (po ov ,
K1obou ), wild rtit (3ootty and 'cthion ), h ctorc (ttl lowfY ),
and Mold go (inch, tho1ich Qoiccion~ ? Tho mole provod
to b unnoooopt b o to to i:t'ioinl i uotioh with foot-and?r oath
dicot1co (Fro aka1). dwart i ~uoooodod with crtittcia1 inLootion of
bats ? Ainon . crr 11 oxporimant i 1obardtory niiiia1 ~, tho ino old,
thQ rabbit, o whito tnatai , otd tho ho oho aro oucaoptiblo to
loot- t-n ouch diaoo o ? Men is t~ aartditio 11;r suacoptiblo to
tomb-ant"aotth dinoaso?
The da roo of aucaoptibiiity to ootwo t?~ o+Ft , Fttr o o
voriou c typos c love to-f otr tod tiniin 1 c ii rtcn-urn ltortn On the oaio
htand, rot o11 ortiz tat c ct tho o,mo typo, sumo p ;iblo to Foot-dnd-
n~auth disctcc, oro aquo11y sai itivc to tho d oasa in tho ar mo
opi~eatic yituation. Indivi taol too i t oo, connoc i with tho
bio1o ical poau1ioa'itias of tic or? tth :sn (ooalatitution, sox, o,
do roo of 1'ocdir1C)., also hos ci nificanno? 11i h1y brod podi rmd
otock are oonoidoro t more som itive to foot-and-mouth ditac is thdri
ioo&1 indicnoua atoak?
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 Declassified in Part - Sanitized Copy Approved for Release 2012/03/22 : CIA-RDP82-00039R000100170030-0
o~tt~o 'M
: nth sr;: p :Yli,i 1n . fs~t3 o~ttbt ffi0'!t!'C ~'* tl~~ 41Mif~t~~lt'~d'1d~ftM
the Or~av
d~6o~~0 o!i~oot:o ~n ono o
~~r~ov-~
~a~~`~~ ~rrd~v~d~t
~
o~o~rvod ~ ~otii'~ v v~h
roh 100 noraeflt, whi~o tti trows ~~~~irt6 tho rtixk tt~
ob r rl ff iiat.tvidu~
dint cal n, th i ik iiliik
~~,~tt* ~~i -~ .
b ;c ils~. Q ifs pr , i ~t wRh re f u t cry t1, rt. ` v trit :VrQ i
h ornLi,
{
Thr virus vt r i`f{ ?"r , r t#~ w; yy~ >