THE WORKS OF THE INSTITUTE OF INFRAMICROBIOLOGY OF THE R.P.R. ACADEMY

• Document Type: CREST
• Collection: General CIA Records
• Document Number (FOIA) /ESDN (CREST): CIA-RDP80T00246A019200640001-8
• Release Decision: RIPPUB
• Original Classification: U
• Document Page Count: 499
• Document Creation Date: December 22, 2016
• Document Release Date: December 6, 2011
• Sequence Number: 1
• Publication Date: December 7, 1962
• Content Type: REPORT

Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
Next 1 Page(s) In Document Denied
Iq
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
THE WORKS
OF THE INSTITUTE
OF INFRAMICROBIOLOGY
OF THE R.P.R. ACADEMY
(ABSTRACTS)
1949-1961
F ~ ICI ~ Ufl ONLY
GPOUP 1
STAT
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
THE WORKS OF THE INSTITUTE OF INFRAMICROBIOIDGY
OF
THE R0 P. R. ACADEMY
Abstracts )
1949 1961
FOR OPEC USE ON 11Y
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
CONTENT S
li
Introduction
I.- General inframicrobiology
1
56
e
I
o 1
II,- Viruses and tumors
57
- 86
0
0 31
IIIe- Dermato-viral diseases
87
94
s
o 47
IYm- Viral hepatitis
95
- -18o
? 52
V e4? Enteroviral diseases
181
-223
.1o8
VI om Neuroviral diseases
224
-339
0136
VII Respiratory viral diseases
340
?50o
e205
V IIIe-Animal viral diseases
501
-587
.299
IXomRickettsial and pararickettsial
infections
588
.643
.365
Xe= Varia
644
-722 ?
e395
XI"I- Monographs
1
- 18 e
9439
Alphabetic index
FOR OFFICIAL
451
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
INTRODUCTION
The abstracts of the investigations of the Insti-
tute of Inframiorobiology of the R.P.R.Aeademy in its 13
years activity, is both a summation of the persevering
work, carried out by the research workers of our Institute
and a hommage brought to those who have helped to obtain
these results.
They also reflect the wish that the works of the
Institute should, be known by specialists at home and abroad,
by all those who are interested in our specialiya
However, in the first place, we should like.to
express by this publication, our sincerest thanks to the
Rumanian Workers' Party and Government, that fully under-
stood the importance of inframicrobiology, already 13 years
ago, and created and endowed the Institute we are nowwork-
ing in; that have moreover decided to develop and build a
new institute, which will be very soon ready; that have
given'us immense possibilities for fruitful researches,
that have brightened our work and life as investigators in
the unknown world of virology - a thrilling work. in itself-,
th4t have honoured us by entrusting us with new tasks, when
the first medical problem was stated in the directives of
the IIIrd Congress of the Rumanian Workers' Party : the
problem of viral diseases.
Our institute, the only large unit for the study
of virology in the country, has assumed the task o an
authority in the methodology of our speciality, of a center
for graduate and specialization teaching and, in answer to
the=various appeals of the tutelary bodies, of the obli-
gation to investigate. the most varied problems. It is for
this reason that the number of problems and themes studied
0" in, - M
fUR
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
II
iii relatively great.
In the 13 years of its existence, in the Insti-
tute of Inframicrobiology staffs of specialists have been
trained, instruction has been given and research work has
been carried out; research destined to bring its contri-
bution to science in the country and abroad. Science has
been linked to practice by the help given in the field
in the control of viral diseases and for the improvement
of public health; useful ideas on virology have been
broadcast by lectures, article, papers and booklets.
Fundamental ,sca.entific research, this reserve
of future technique as it is.called by Lenin, is our
principal aim, of all those working in the institute.
Research however has never been separated from practical
applications. It is true that many works have been pub-
lished concerning results obtained in problems initiated
by as (ultrafiltration, megamononucleosis, synchronophy-
laxix, septineuritis, hydropigenous neurovirosis, tissular
immunity, paraimmunity, the biophitic state, viral-onco-
lysis, metallized viral vaccines, the plurality and varia-
bility of the hepatitis virus, etc.), or in problems that
have long been studied (cellular inclusions, inframicrobial
histopatholo?y, vaccinia, rabies, poliomyelitis, herpes,
inframicrobial variability, etc.), or, more recently ,
(the viral etiology of cancer, Coxsackie, psittacosis,
A..P.C., ECHO, interferons, in vitro cell cultures, electro-
phoresis, chromatography electron microscopy, labelled
atoms, Pavlovism in virology, cytochemistry, biochemistry,
viral biosynthesis, ionizing radiations, etc.), but it is
none the less true that in view of the actual requirements
of medicine in our country, we have been constantly preoccu!
pied by the applications of science as demanded by our go-
verning bodies.
We shall recall the vaccines studied against 14
human or animal viral diseases : small pox, swine plague,
avian plague, fox encephalitis, avian diphteria-variola,
0
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
III
hydropigenous neurovirosis, influenza, ,psittacosis, rabies,-
mumps, Aujeszky's disease, lymphocytic meningitis, foot and
mouth disease, tick-borne encephalitis; another two vaccines
are under study: APC and anti-measles vaccines. Some of
these are already widely applied in the population or for
the immunization of animals and birds.
Numerous diagnostic tests for viral diseases, some
of which original, may likewise be mentioned. Instruction
given in the field in. various centers, to`a great number of
doctors, in view of the application of these tests, has
likewise been one of our objects. Hundreds of work-days
have been spent, at the demand of the.Ministry of Health
and Public, Welfare, in order to come in aid to epidemiolog-
ists. Two of our units, at the Colentina Hospital and Hos-
pital of Contagious diseases no.2,-perform diagnostic tests
and virus isolations from patients
The isolation and identification of viruses in
clinics or.,.in field work have made...evident for .the first
time in our country the etiologic agents of certain human
or animal viral diseases : ECHO, APC, tick-borne encephalitis
virus, pneumonia, fox encephalitis,;:-epizootic abortion, pneu-
monia of calves, ornithosis, recurrent meningitis viruses
and the agents of various human and''animal neor.ickettsial
affections, etc.
The':scientific works of the investigators of the
Institute,numbering about boo, deal with general and spe-
cial virology, in which 24 human viral diseases and 13 ani-
mal and insect viral diseases are studied: herpes, vaccinia,
rabies, sueta miliaris, epidemic hepatitis., lymphocytic
meningitis, measles, German measles, poliomyelitis, influenza,
encephalitis, atypical pneumonia, parapoliomyelitis MM,
Coxsackie, mumps, infectious mononucleosis, infantile in-
fectious lymphocytosis, neorickettsial pneumonia, recurrent
viral meningitis, ornithosis, tick-borne encephalitis,
adenoviral and ECHO infections, malignant lymphogr.anuloma-
tosis , moluscus contagiosum; fowl plague, swine plague,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
Iv
fowl pox, distemper, Newcao.,., disease, clavella, Aujesky's
disease, fox encephalitis, canine hepatitis, swine enzootic
pneumonia, anemia of horses, hydropigenous neuroviroses of
fish, jaundice of silk worms.
In epidemic hepatitides, or1'e of the most impor-
tant contributions is the fact the plurality of viral hepa-
titides has been established by the description of new forms,
sclerogenous hepatitis and subsequently a hepatitis due to
virus NHA. In the course of the investigations carried out,
inclusions characteristic of Botkin's disease were discover-
ed, as well as the topography of the virus in the hepatic
cell (St.Nicolau, R.Portocald at al.); the electronooptical
morphology of the sclerogenous virus was established (St.Ni-'
colau at al.), its hemoagglutinatitg properties (N.Cajal at
al.), serologic differences between the sclerogenous and
Botkin's virus (St.Nicolau at al.), the distribution of the
sclerogenous virus in the human organism (N.Cajal at al.),
the duration of the elimination of hepatitis viruses from
the organism and the presence of virus carriers (N.Cajal et
a1.); the "zone" phenomenon has been described and the non-
specific factors that interfere in the hemagglutination and
hemagglutino-inhibition reaction; it was possible to isolate
and maintain in serial passages the sclerogenous and. Botkin's
virus in embryonated eggs (St.Nicolau at al.); detailed morpho-
logic studies were carried out establishing the general cha-
racter of the viral disease (St.Nicolau at al.); different
biochemical aspects of the affection were studied (R.Porto-
ealA and V.Boeru); serum aldolase was determined and its
diagnostic value established even in the unapparent forms
(N.Cajal at al., D.Mitroiu at al.); a comparative critical
study of the various diagnostic tests were performed (N.Ca-
jal at al.); hepatic and muscular aldolase was determined
(P.Athanasiu and N.Cajal); the diagnostic value of the A.V.B.
test and agglutination of Rhesus and chick red blood cells
in this viral disease (G.Balmu$ and D.Mitroiu); interesting
epidemiologic studies were also carried out (St.Nicolau,
G.Marinescu, N.Constantinescu, N.Cajal, etc.) -
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
V
Spontaneous variability of hepatitis viruses under
Aatural conditions was demonstrated and the k,!urality of the
epidemic hepatitis was interpreted in the light of this
variability (St.Nicolau'and N.Cajal); certain strains, that
appear to have etiologic relationships with this viral
disease, were isolated in cell cultures (D.Mitroiu and co-
workers); a study was carried out on the relationship
between canine and human hepatitis (Surdan j j,: - 1ti, ) , and murine
hepatitis viruses (N.Cajal e.t ,.io) etc,
lost of the results of lumanian investigations
in the fiel& of epidemic hepatitis are summed up in two
monographs publishes by the R..e.x. Academy. Numerous works
have been published in the periodicals at nome and abroad.
For the resu.its ob,ained in -The field of epidemic
viral nepaUtiis, the i Class State Prize of the R.Y.R.
was awared in 1953; aftd for special biochemical studies in
this connection, R.Portocala received in 1957 the "V.Baber?"
Prize of the R.r.R.Academy.
Recently, at the International Congress of infec-
tious Pathology in Milano, we had the satisfaction to'find
that our conception on epidemic hepatitis, concerning the
plurality of the virus and its variability in natural condi-
tions, have been accepted by all the specialists in this
field.
in influenza and other viral diseases of the res
piratory tract, various influenza virus strains -- isolated- _.
in the country since 1953 and up to date were investigated (A.
De,i sib aW,);tne dynamics of hemagglutination-inhibition
ax~'!bodies were studied in the population, thus confirming
the data regarding the variability in nature of this virus (A.
Der Otil it'a3..) An ezticient ovovaccine was prepared in a
close-circuit apparatus, specially deviced in the Institute
and successfully applied in the field (A:Derevici); a
diagnostic test based upon the precipi anion phenomenon
was developed (A.Derevici and N.DragAnescu).
A study was carried out on cytologic diagnostic
methods of the Koleaditzkaia or Yanthier.type (Al.BroniVk.i)
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
VI
and a. critical experimenta_ ?y on diagr.. o s,ic tests
(tim8araVearbu and N.Draganescu). J pidemics due to viruT A
(Asia) were described (A.Derevici et al.); for the first
time in the country, influenza virus type D was isoya-.e
(D. rarchila et al.). Modification of the influenza virus
was obtained by vegetative cross-cultures with otner viruses.,
especially neurotropic viruses (P.Stroescu, Ai.retrescu,
cord,ical dynamics in the course or ani.i-influenza vaccina-
tions in man anti animal and circulatory and respiratory
changes in the course of that disease was studied (L.MI rza
et al.). The .role o1 i-Ai influenza virus in the etivpathu-
geny or the corticomseptic syndrome in infants was demonstra?
yed (N.Cajai et al.); the action of embryonic fluids on
6he influenza virus was established (N.Cajal and M.Cep-
leanu); investigations were carried out on the experimental
disease in animals with gonadic hypoactivity (C.Baba and
N.Cajal); investigations were done on influenza virus
cultures in the de embryonated egg (m. uepleanu, Y. c.opelo-
vici); anti-influenza immunity in animals previously in-
fected with the 'vac'cita virus (DaSI.ra'eanu and B.Fuhrer);
influenza morpnopathology in the suckling mouse (G.Mari-
nescu et al.); the virus in non-epidemic periods and iso-
lation of 'a virus with neurotropic properties (Ai.BroniVki
et al.); interference between various influenza viruses
(G.Danieiescu); evolution of the experimental disease in
:Largactyi treated mice '(N.Cajal et al.). From cases of
swine influenza, influenza virus type A was isolated,
demonstrating the role of these animals in the spread and
maintenance of the influenza infections (N.Cajal and M.Taga);
serum antibodies were studied by microelectropnoresis
(R.Portocala and V.Boeru); interesting research work was
carried out on replication of influenza virus, obtaining
biosynthesis of the viral elementary bodies following
introduction in the embryonated egg of purified ribonucleic
acid extracted from the influenza virus (R.Portocala et al.);
the electronic aspect.oi' the virus thus obtained was desc.cibea
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
VII
(R.Porto,~.ala et al.); the action of ribonuclease on influenza
lirus mui'Uplication (Y.Copelovici and 1".Cep)eanu); the
p1ysical mixture or two virus types was described, thus
-explaining the mixt virus (R.Portocala); the action of
vitamins C, PP and B was studied (G.Popescu); interference
and the mechanism of action of interferon (G.Danielescu);
the allergic factor in influenza (A.Derevici et al.). The
neutralizing action agains-u -tae influenza virus of bee
royal jelly was discovered (A.Derevici et al.); dia.gnastic
sera and anti?eus were prepared'(A.Derevici); new hype ox
virus that produces a disease similar to influenza, the Boto
virus, was isolated (A..Petrescu et al.); the action of
ionizing radiations on influenza and Hi an-6lboaieS was in-
.-estigated (O.Burducea and M.Cepieanu), etc.
For the first time in the country, APC viruses
were isolated and investigations were carried cut on Lhe
alterations produced by Leese viruses in human embryo cells
(I.Aderca and M.Ianconescu) and on anti-APC antibodies in
the population (A.Derevici'-and co-workers;-M.Gruia and co-
workers).
The infectious role of DNA extracted from APC
virus was established (R.Portocala et al.); the sensitiviuy
of different cell types t.o Leese viruses was studied (L.
Preaescu and co-workers).
It is likewise for the first time that germs of the Gi-
I,M~A neorickettsial group were isolated in the country and
s-,uaiea in the electron microscopes (D,Si?lueti,al.) , these germs
T,=re trasmitted to the cotton rat (N.Drag:nescu, D.Sara ea-
rIL); ocular lesions produced by neorickettsias (G:Marinescu)
were described for the first time in the country, psittacosis
pneumonias were detected (~.Sto ) and numerous ornithosis
strains were isolated from man and different animal species
following epidemiologic and epizootologic surveys (D.Sar#eanu
C. ;urdan et.. al.) ; a metallized vaccine was prepared, as
well as antigens and diagnostic sera (D.53rateanuet.,al.);the
etiologic correlation between these germs and various cases
of cardiovascular diseases was studied (St.Nicolau et al.),etc.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
. VIII
As regards the --Le of rickettsias and pararickett?
Sias L, the e- :L,j.,wj ;fir of cej.? . n .tauma:a di5uases, our invest-'
gato:cs ioye, -shown tie role or- these germs in the etiology
of certain cardiovascular affections (infarction, arbe.L1tis,
migratory pniebitis, etc.) ~St'.Nicoiau, C.Surdan et al-) q
gastric ulcer-(8.Nicolau, Ai.re-trescu et al.), infantile
.pneumoencephalitis (N.1ara ;arHscu et al.) , certain chronic
respiratory affec,,6ons - (puimonary sclero-emphysema", chronic
bronchitis, bronchiectasis, etc.), the pathology of preg-
nancy, mortinatality and conge malformations (N.Cajal,
C.Surdan et al.) ; stet' - :_c investigations with antibiotics
or the tety? Q*c~e:9 group have yielaea important data, which
on the one hand confirm the rickettsial or pararickettsiai
ebiolugy of these affections-~ and on.the ocher, open wide
prospeebs in the prevention and therapy 'of these aisee&ses,
that consc?itute an importanb problem of public heal.bhe
Rumanian invebbigation on influenza were synbhebiz-
ed by A.Dei-evici in*her monograph "G?ipa" aspecte din pr,ob-
lema (influenza --aspects of the problem)., publishes. by-
tne R.?PBt.Ac ademy, those concerning ornithosis will appear
in a mono g,-apn written. by 1):.SArAVeanu,. ana those on adeno-
viruses in amonograph by -A?Deievici, in the press.
Field activity and'investigations during the in-
fluenza epidemic of 1958 have been summed up in the volume
""Gripa,.date asupra epidemiei ain 1959 ( Influenza m data on
the 1959 epidemic), published by zditura Medical&.
In rabies,_ the. comparative action of various
antirabies vaccines was studied (bLL Constantinescu ?~;,al.)ga
vaccine was prepared with a fixed methylic alcohol attenuat-
ed virus, suspended in mineral oil (S?.Nicolau.ot.,..-.p);'?closed
rabies" and the important role of this notion in the pro-
phylaxis of rabies were described (S.Nicolau and N.Constanti-
nescu);. the priority of V.Babes in the discovery of rabies
inclusions o the Babes-Negri bodies o was established (Sts
Nicolau and M.Babes). Thus, inclusions have.been describes
in the retina of naturally or experimentyinfected animals
(x..Fortocala and N.Cajal), as well as in the carotid glomus
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
IX
(P.Athanasiu et al.); histopathoiogic studies of the nervous
vegetative system in this viral disease have likewise been
carried out (P.Athanasiu et al.); a rapid method of diag-
nosis by the phase contrast microscopy was aeveloped (N.
Cajal and S.Mateescu); cases or rabies with a long incubation
were studied (S.Nicolau.et al.); the part played by medicinal
and reflex sleep in the evolution of this viral disease
(S.Ricolau et al.); the' early onset of rabies detected
by means of conditioned reflexes (L.Mirza et al.); the
unapparent disease in the course of antirabies vaccination
detected by means of the method of conditioned reflexes
(G.Danielescu et al,); the role of cortical reactivity in
the-course of anti-rabies immunization (1. Cajal and G.
Popescu); the important part played by association of
immune serum in the prophylaxis of rabies (N.M.Constanti-
nescu et al.); inclusiogenesis in animalb subjected to the
action of ionizing radiations (S.Mateescu); "the law of
intervals" applied 'o anti-rabies immunization (N.Constan-
tinescu et al.); the comparative immunogenic value of the
Flury strain and the glycerophenol treated vaccine was studied
(L.Paeeu',et balo) : _. a silvered active vaccine was prepared (St.
NicolaaetaL) and" dissemination in the organism of viral
elementary bodies labelled with radioactive silver was
followed up (Q.Nicolau e0); a new ft agnostic test - the
peritoneal test - was devised (S.Nicolau, N.Constantinescus)
and the following studies were-carried out : the action of
cortison, X-rays and isotopes on experimental rabies and
antirabies immunity (N.Cajal), rabies in the hamster, the
sensitivity of.this animal in experiments with rabies
virus (N.Constantinescu. et. i7 )eq;0Recently, St.Nicoiau, N.
Constantinescu and N. Cajal have published their monograph
on Turbarea (Rabies) (The Publishing House of the R.P.R.
Academy).
In poliomyelitis and related diseases, a detailed
histopathologic study of the sensitive nervous system was car-
ried out (St?TicoliLu 6t:a1); a histologic study on experimental
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
poliomyelitis with the Lans;.ng virus (El.Lissievici Oprescu);
the poliovirus wart cultivate: in HeLa, Detroit 6 and human
amnios cells and comparatively in human embryo cells (I0Adeb_
.ca et,,al.);poliomyelitic myocarditis, hepatitis, nephritis
and pneumonia were described (G.Marinescu et al.); t:e
incidence of anti-Lansing antibodies was established in the
population of Bucharest, and the origin and significance
of these antibodies discussed (N?Cajal et al.). The idea
of paraimmunity in herpes-poliomyelitis'was developed (Sts
Naoo)lau and M.Ianconescu); a study was carried out in cell
ciz~,Lures on antibodies to the three poliovirus types, in
the inhabit.en'ts of various towns and villages in the couri=
t.Ly et al.) ; the e voiutiun of antibodies in children
vaccinated with Salk vaccine was followed up (,N.Cajal et al,);
electrophoretic modifications in vaccinates persons was
studied (G.Marinescu et al.); the lesions produced by polio-
virus in embryo and human amniotic cell cultures (i.Aderca
and M.ianconescu); for the first time the poiio vaccine
prepared in the Puiiomyelitis ins'r itUue of Moscow wizh
modif.Led Sabin strains was appiied in tyre country and eli-
mination of he virus and serum tests were studied (N.Cajal
et al.); the action of radioactive isotopes on type seru-
neutralization antibodies (O.Burducea); the poliovirus neu-
tralization test was described (St.Nic?iau et al.), etc.
Numerous Coxsackie virus strains were isolated and the etio-
logic relationship with poliovirus studied; the aspect of
the myofibrils of mice infected with the Coxsackie virus
were investigated in the electron microscope (R.forzocala
et al.); muscles extracts from Coxsackie infected animals
(Rorortocala et al.); Coxsackie A. virus was transmitted to
beta and gamma X-ray irradiated adult mice (N?Cajal and
The rirsz EeHu virus strains were isolated in
nnn-t;r;y (M.Gruia et al.; i?Aderca et al.) and investi-
; n prri.ed out on anti ECHu antibodies in children's
con . -Yn e in Buchare t, (M.Gruia, Y.Copelvvici) and HAS
anti r)-.d:r es (M0Grua.a et al.; G.Marinescu). ECHO virus strains
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
XI
were isolated from various cases of .fant' :`.e enteropathies
(M.Gruia at al.), etce
Acad. St.S.Nicolau, acad.Al.Radulescu, N,Cons-
tantinescu, N.Cajal and G.Marinescu have drawn up a documented
monograph containing foreign and Rumanian works in the field
of poliomyelitis.
In herpes it was possible to transform the relaps-
ing herpes virus into herpes simplex virus (St.Nicolau at al.);
the characteristic lesions produced by herpes virus in the
retina was studied (R.Portocala and N.Cajal); investigations
were carried out on-the experimental disease in-animals with
a modified reactivity of the cerebral cortex (N.Cajal at al.);
the influence of vitamin C on experimental herpes in the rab-
bit.(N.Cajal and C.Baba); the evolution of herpes in animals
with aloxan-induced diabetes (C.Balmuq ar,' N.Cajal); herpetic
"diabetes" was described in rabbits (R.Portocala and N.Cajal);
the relationship between herpes and poliovirus (St.Nicolau
et al.); the evolution of experimental herpes under the
action--of cortisone (N.Cajal and G.Popescu), and gonadic
hypo- and hyperactivity (N.Cajal and C.Baba); antiherpetic
immunity in irradiated animals (S.Mateescu); virus isolation
in cell cultures (M.Ianconescu et al.); the incidence of
antibodies in the population (M.Ianconescu); lesions produced
by herpes virus in human embryo cells (I.Aderca at al.).
In lymphocy-ticmeningitis, numerous virus strains
were isolated (A.Derevici at al.) and an ample clinical,
epidemiologic, and-experimental study of the disease and
histiopathogenesis of the experimental disease was carried
out (A.Derevici et al.). Comparative serologic studies were
done, showing the best diluting fluid for experimentation
with these viruses (N.Cajal and Y.Copelovici); studies were
carried out on the etiology of meningitis cases hospitalized
in Bucharest (M.Gruia et.al.) and on methods of etiologic
diagnosis (N.Cajal). A virus producing recurrent meningitis
was isolated (N.Draganescu) and a monograph published on
lymphocytic meningitis (A.Derevici).
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
XII
In encephalitj ,;,o, several vi uses were isolated
and vast serologic and histopathologic studies carried-oat
on various types of human encephalitis and especially tick-
borne encephalitis (N.DragAnescu et al.). In connection
with this disease, the incidence of complement fixing anti-
I.-bodies in forest workers was followed up (D,SArAVeanu et al.)
and a metallized vaccine developed (St.Nicolau at al.).
Multiplication of the encephalommyooarditis MM virus in tis?
sular cultures was likewise studied (N.Cajal et.al.); an effi-
cient metallized vaccine-was prepared (St0Nicolau, N.Draganes?
cu at al.)
In mum, the viral elementary bodies were studied
on smears obtained by puncture'of.the parotid glands and
stained with phenic isamine(S.t-.Nicolau and co-workers); the
non-specificity of the inclusions found in the salivary
glands was demonstrated (St.Nicolau et al.); numerous mumps
virus strains were isolate'd' 'in .embryonated eggs from the
cerebrospinal fluid of patients with the parotid or meningeal
forms; comparative investigations on the value of serologic
reactions were done; the behaviour of the virus in the
embryonated and 'deembryonated eggs (M.Cepleanu); a vaccine
was prepared with autochtonous strains, applied in the field
(YoCopelovici et al.) and the vaccines prepared according
to various techniques were studied comparatively (Y.Copelo-
vici and M.Gruia)?
In Aujeszky's disease, epizootological and clinical
investigations were carried'out (C.Surdan at al.); the spe-
cific histopathology of the natural and experimental disease
was studied in detail (St.Nicolau at al., C.Surdan at al.);
adaptability of the virus to the chick embryo was investigated
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
XII I
and attempts were made at preparing a vaccine with the
c .itura virus (.Suhaci et al.); an active hyperimmune serum
was prepared experimentaly (v.Tomescu et al.)
in fox encephalomyelitis, epizootological, clinical,
experimental and histopathologic studies were carried out
and a vaccinescurrent-11.y applied, saved many breeders in fox
'breeding farms; the etiologic unity of fox encephalomye-
litis and canine contagious disease was demonstrated and the
relationship of this virus and human hepatitis viruses in-
vestigated (C.Surdan et al.)
In vaccina new method for the improvement of
vacc3 in lymph production was developed and the notion of e-
p1thelio-vaccinia and pyovaccinia were introduced (St..Nicolau
at al,). Investigations were carried out, on the cation of
serum gammaglobulins in the immunization process (R...Porto-
cala and N.Cajal). The influence of the vac r inia virus on
the development; of anti-Eberth antibodies, hemolysins and
the body weight of e-.porimental animals was studied (N. Ca-
jal et al.); the evolution of vaccinia and antivaccinia
immunity in the rabbit under the action of cortisone (N.Ca-
jal and Y.Copelovici); the action of ionizing radiations on
experimental vaccinia and immunity induced by this virus
(S.Mateescu et al.); the influence of vitamin C and cortisone
on vaccinia virus cultures in the embryonated egg.(C.Fopescu);
investigations were made on the development of influenza RAI
antibodies in animals previously inoculated with the vaccinia
virus (D.Sarateanu) and the morphologic aspect of the virus
in the electron microscope (St.Dumitrescu and N.l.Ionescu).
in zona zoster, the morphology of the virus and
histopathology of the skin lesions were described (St.
Nieolau et al.) and the question of the unity or dualism
of zona and varicella discussed.
In fowl pox an important contribution was brought
by the investigations of the institute to the immuno-biologic
relationship between the various virus strains (V.Tomescu et
al.) and the aetailed study of specific inclusions. The
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
vaccine prepared was currently and massively applied in
tile 1-1. -. . DeIa ~,- I st al, w apec1iic _k las ions were
car.LJ. d -,)U4 .At3la~a33a~iu et a1'.)
in measles anu. German measles, the morphology of
the virus was studied on smears stained by pnea?sl e
(St.Nicolau et rel.); the morphopathology o";qs:t iieasies
encephalitis was likewise investigated (G.Marinescu et a10
and German measles transmit yea experim. ita1 to Fah : r l; it
(NoConstantinescu et al..). Attempts were made to prepare
an anti-measles vaccine with the virus inactivated by immune
serum (St.Nicolau at al. end comparative studies
were carried oy.it on cultivation various mumps virus
strains in a.ifferent celis, in vitro rI.Adevca ana M.
Cepieanu), ? aut,ochionous strsins were isolated (M.Cepieanu
at al.) ana vaccine now being prepared accoraing to the
Katz-Milovanovici formula (I.Aderca and M.Cepleanu).
in swine plague, the investigations had in view
especially specific prophylaxis (V.Wynochradnyk,S. Mihail~g
at al.; V.Tomescu at al.); the comparative value.of vaccines
adsorbed on aluminium hydroxide and those inactivated by
crystal violet was investigated (S.Mihaig at al.); the
duration and intensity of immunity conferred by egperimentai
vaccines were established; vaccines were prepared from
organs other than those usually employed and a vaccine using
.Lapinized virus. was tested (S.Mihaita et al.); the reversi-
bility of the lapinized virus (L.yascu) and the distribution
of the vaccine virus was investigated (izheorghiu)
The results of Rumanian investigations in this
viral disease were summed up by i?Gheorghiu, S.Mihaiita and
co-workers in a monograph published.,by the R.PoR.Academy.
For their important contribution 'to the problem
of swine plague, V.Wynochradnyk was awarded the State Prize
for 1954.
In avian pest and pseudopest, numerous virus
strains were isolated and typed (C.Su rdan at alitl.Suhan
et al.); interesting immunomprophylactic studies were
conducted and specific vaccines were prepared and massively
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
T
l -it tie country (So
%pplie , w' h the best results hl-X3 ,gI
,,i:1.aci et al.); morphopat o4.`agic study OT ,tc . - - ..bryo
ie:Aons (Serb ,esc' . et ?,) an avian pest a.mti *,erum was
T)t?9pared in turk y,s. (I Suha?i et al3) A b.i.s4opat oi;ugic
study or the nervous sys.-Rem 3n m?n:e exJe rinienta-L-L.y infected
with an avian pest strain speci.:.~ Jy adapted to this animal
spL,cies (R0Porttoc;a1_, and N0Cajal); t;:; investigations had
In view a comparative study of the value of antipast vaccines
prepared with live modified viruses (i.Suhaci et al.) and
the distribution of the vaccine virus,
For his exceptional contribution to the problem
of specific prophylaxis in avian pest, l0Suhaci was awarded
the R.P.R. State rrize for 1954.
In the problem of viruses and tumors., an importan e,
contribution was brought to the to.Llowing questions : the
oncolytic aouion of eeitdin viruses (Sr 0!' ca4au, EeLVastac,
B?Anagnoste), the correlation between viruses and cancer
(S0Nicolau), the study of :Ehrlich tumors, proving their viral
etiology (.E?Nastac et all.), the evolution of Gudrin sarcoma
in animals previously infected with the vaccinia virus (N.
Cajal, E?Nastac), the combined oncolysing action of certain
viruses and cytostat cs or narcosis (O Cost.chel and E?Nastac);
the action of certain 'cheiating products on experimental
.r.ors (G ?Baimug et al.).
An agent with "double antigenicity was obtained by
inoculation of adenovirus type 3 into Ehrlich's ascites
~: .rcinoma; the action of ribonucleic acid extracted from
,rious viruses on several experimental tumors, was investi-
gat,edm
In the problem or the viral-etiology of human and
animal leukemias, atrpmpts were made at isolation of a viral
agent from the blood and organs' of animals or patients suffer-
ing from leukemia; the pathogenicity of murine leukemic
products from AKm mice for the rabbit, guinea pig, mouse, was
demonstrated; anatomo-pathologic lesions were described and
their transmissibility by serial passages (EjNastac,B?Anagnoste
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
XVI
et al.); in human leukemip our investigators showed the
pathog6nl..city of Jiurnan leuks.. ,zap; products ';ar the white hybr: a
mouse in zwb .ch a serially transmissible disease with histo?
pathologic modifications was producea (E.Nastac et ale). At
the aame time, studies were carried out in the electron
microscope and on the evolution of experimental leukosis in
animals irradiated with radioactive isotopes (N.Cajal et alp),,
Two virus strains were isolated from cases of,
human reticulo-sarcoma; the disease transmissible in series
in the embryonated chick egg was studied in the optical and
electron microscope; eJ.e-"gatary bodies were observed in
lesions of the c,kaoor:o-allantoic membrane (N.Cajal and M.
Cepleanu).
In Hodgkin's disease, several agents transmissible
in series were isolated in the embryonated egg, producing
lesions at the level of the embryos and cytopathic effect
in tissue cultures. Their reaction to physical, chemical
and biologic agents suggests the viral nature of the isolated
strains, that form the object of ample experimental morpho-
logic and serologic investigations (A0Schachter, N.Cajal,
M.(;ep.Leanu and Y.Sorodoc).
Acad. St.Nicolau published a monograph dealing with
the correlation beuween cancer and viruses; and N. Cajal and
E Nastac wrote the chapter on viruses and tumors in-a treatise
on oncology published by bhe Editura Med.icalap
Apart from these important studies, the inframicro-
biologists of the institute have also carried out numerous
investigations in other problems, such as ; infantile sueta
miliaris (St.Nicolau et al.), hydropigenois neuroviroses of
fish (St.Ricolau et al.), jaundice of silkworms (A:0Derevici),
infectious anemia of horses (C.Surdan et al.), distemper
(St.Nicoiau and C;06ur?dan), unapparent viral diseases, spon-
taneous inclusions (St.Nicolau, R?Fortocala, N. Cajal), in
infectious mononucleosis (D?Sarateanu, G.Marinescu) , infectious
lymphocy4osis (N?eajal et"al., G.warinescu), Kurloff's bodies
(St.0Nicolau et al,), cytomegalic disease (R.Portocal& et al.),
certain eruptive viral diseases (C.Mitroiu _et al.) and foot
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 : CIA-RDP80T00246AO19200640001-8
soon hA~ At tiahe'` bro'adcasting' stet Ora, t8 ev sion ystaat: on:,:=
and R.iftimovici More. than 'too lectufres on ~%~rus:eis? have?
Babee, by St Ni~colau )and-'3) The wor1d..of ?v use$, Eby t.C,aja1
view "S&udii si cercetari de inrramicrobio;logieft (Stud es .
and investigations in inrramiorobiology), (-5895 pages) ?:
Two bookie bs for the populaEizatiot~ of science
b.ave., also- been published: 1) Microoes and :viruses: ,. 2) V.
.(in the press); 27) Mumps, etc.
The institute has published 42...aambers of the re-
,.F ,Y iser s ,a.
microscope techniques in the press);. eo) 41CketG$
press); 4) Adenovirals dise;as,es. (in the press);;: ~ ) le~ctron
l d
i
,
r inr_rau.microbialO V ;(in..the.
biology. 2o) ale te_nt* of. gezserr i nfratio }robiOLOgY}:
;
of, special infram crobio;1,ogy; ;e2),:.Orn Ftrhosis
dl.)" Elements
inframicrob ology ('in t;hinrese ),;, 19), he,et.ures_, on. inf am cro,;
17) Influenz clefs on. the:,. 1959:, epdemic-; 1$) t'roblems of
disease ; 1) Swine p-t;f 16) . fihey jaundice of : siaga lk worms ;
Diseases transmissible to man from animals; . il) Polfiomye Otis;
12) Viral infectious hepatitis; 13) Rabies; 14) Fo.ot anRd mouth
seases; 9) 1nr'ectious-lymphocyt?s;is and mononucleos;is;.. l.oY)
?/) viral hepatitiae.s,;, 8) Laboratory diagnoses, of v,rq4, d1-s:
phalitides; 3) Ly?nphocytic...meningitis.. 4). Influenza; 5) Eie?
menus of general l niramierobioogy, 6) cancer and 'viruses;
of influenza; 2) Soviet invesi; ggtions, in. the field of ence-
or are in the, press: 1) Soviet investigations ,in the fish.
Thus o monographs < heave _, be?.n written and.' pub 1. she q 9
sis were likewise pub1ished.by.G.Marinescu.
the works concerning mononucleosis and..,infectious.,lympnocyto-
in his monograph Febra,;aftoasa. (F,oot. and.mouth; disease.) , andz:\
latter disease-,were summed up by V-eTomescu- and,,,collaborators
(V Tome seu-,. et al.;) manyinteresting investigations were
,.:
carried out. r Most of the..Rumanian investigations in the
and mquthkxdsease, for which, an=;efficient vaccine was- prepared
of science.'
published` ii newspaper's` anti ?revieW6 fb - ?t~h p ;pn'iariza~t}io.,nxa:
hundreds5 of scientific articles, clecklakra'tiahs tc: have been.
public lecture halls in Bucharest and, the prvinc'eis,.F.. ee the percent-
age of mortality and prolongs the period of incubation and
the life of these animals.
Larga-ly administered by intramuscular route
to the white mouse infected with the street rabies virus
does not influence the evolution of the disease, but seems
to exercise an effect on experimental rabies of the golden
hamster,
26. M,CEPLEANU, Y.COPELOVICI. Comparative study of the
culture of, some' viruses' On2embryonstad-7ia; :
deeiabry6hdted"eggs ^R@pett-t@aadnatoth@" S@@sion
of the _ In@t T Of - Ifframicrobiology of the R.P.R.
Academys July 31, 19580
The inoculation of some viruses on the deembryon-
ated egg according to a modified technique, makes it possible
to obtain good results with viruses that develop on the endo-
derm of the chorio=allantoic membrane (influenza virus, mumps
virus, Newcastle disease virus).
This methos.3 can be used for electron morphologic
studies, for investigations on the dynamics of virus multi-
plication, for the study of the effect of. certain substances
on virus multiplication,, etc. Besides being economic, this
method. also has the advantage bf removing the individual
biologic factor of error.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
17
27. G.DANIEI,ESCU. Study of interference in the field of
virology.: St.oerc.inframicrobiol.,microbiol. ,
para2itol., 1958, 9, 2, 223.
The author's report on her dissertation for the
-title of candidate in medical sciences. In the first chap-
ter, many bibliographic data are given in connection with
this phenomenon. In the original part of the work, the
author describes-the results obtained in the study of the
phenomenon of interference in experimental infections caus-
ed by the herpes, influenza and street rabies viruses, us-
ing as interfering and excluded agent the same virus ino-
culated by two different routes at given intervals.
The data obtained led to the following conclus-
ions ;
A weak encephalytogenic strain of herpes virus,
introduced by intravenous route to rabbits, can interfere
with the same virus inoculated later into the brain. The
phenomenon of interference is manifested by a prolongation
of the incubation period by 4?5 days in the animals experi-
mented upon as compared to controls.
Inoculation of the influenza PR8 virus to mice by
intraperitoneal and intranasal route brings about interference
manifested by inhibition of virus multiplication, demonstrated
by the hemagglutination reaction.
The fixed rabies virus, inoculated by intravenous
route to rabbits confers a certain degree of resistance to
the street rabies virus, inoculated into the sciatic nerve
after 24 hours.
28. M?IANCONESCU, I.ADERCA. Technique of cultures on human
amniotic cells, St.cerc.inframicrobiol, microbiol.,
paratitol,.1958, 2, 4, 429.
The economic advantages of cultures on human am-
niotic cells are shown as compared to monkey kidney cell
cultures0
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
18
The cultures are obtained by trypsinization of the
amniotic membrane, with an output of 9-11 x 106 cells per
gramme of tissue. The best culture medium investigated was
Hanks solution with o,b5% hydrolysate of 79% lactalbumia,
20% calf serum and 2oo 2aM 1% glutamin.
296 ACAB.ST,eS.NICOLAU, I.MIRZA. ability of viruses
in antiviral veecines stadted:'.b ''~WUS:,Gf`
ion(
In an anti-viral vaccine, the chemically attenuated
In the experiments men-
titer is of paramount importance.
tioned, the fixed rabies virus inactivated by formol conden-
trations higher than 4 %o becomes non-immunizing and also
indifferent to pre-established conditioned reflexes in rats.
In concentrations lower than 4 %0 ,formol respects
the immunogenic property of the virus and apsets the pre-
viously established conditioned reflexes. Similar experiments
results.
carried out with the influenza virus yielded the same re
It is reasonable to expect that up to 4%o, formol
maintains the virus alive, preserving its property of up-
setting the preestablished conditioned reflexes and its
immunologic potential. A formol concentration exceeding 460
causes death of the germ-and ipso facto failure to react tb
conditioned reflexes and abolition of immunologic properties.
The sensitivity of the conditioned reflexes test
for determining the ' ,Viability of germs in ananviral
vaccine prepared-with attenuating chemicals is clearly
revealed.
3o. R0PORTOCALA, V.BOERU. Electrophoretic micromethod on
1 16.
agars Biochimia A,plicatA, 1958 9 5-+
St.cerc..inframicrobiol, 1958,v.29 2, 167.
By applying a layer of buffered agar on a micros-
cope slide, it is possible to obtain an eleetrophoretic micro-
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
19
method that yields a curve which can be superimposed on
those obtained by the classical electrophoretic methods.
Accurate electrophoretic determination with
minimum amounts of serum can be performed by means of this
micromethods
31. R.PORTOCALA. Electron microscopy in cyto-pathologic
investigations on viral diseases.. Microbiol.,
parazito1 ; epidemiol ',' 1958-T `-193-
A brief survey of the present-day data on the
techniques employed to obtain thin sections for the electron
microscope. The principal results obtained by these methods
in the study of cellular lesions caused by inframicrobes
are succintly listed.
32. I.ADERC` 1, 1,;.IANCONESCU, M.NACHTIGAL. ];or, to obtain
human embryo cultures by trypsinization,
St.cerc.inframicrobiol., 1959, lo, 2, 187.
The authors describe in detail the technique employ-
ed for preparing human embryo cell cultures by trypsinization,,
One to three months total embryos .;.ere used. The output of
trypsinization was 4o x 1o6 cells per gram of tissue. The
following nutrient medium was employed : Hanks and Earle so-I
lution + calf serum + glutamine solution + lactalbu .i.ne
hydrolysate. The cultures develop quickly forming a conti-
nuous layer of fibroblasts, which can be inoculated between
the 4th and 6th day after seeding the cells.
33, I.ADERCA,''..IANCONESCU, U.NACHTIGAL. Study on in vitro
cultivated human cells.
Methods and cytologic stud,Y.
Consfatuirea Interregional. de morfologie,
Tg.Mure*, Jan.18-19, 1959,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
2o
The techniqueSof tissue and cell cultures devised
for inframicrobiologic investigations have led to the deve-
and microbiology. The cultures obtained
lopment of cytology out extremely
by modern methods make it possible to carry
minute cytologic studies. A description is given of the
technique employed for obtaining human cell cultures from
human embryos and human amniotic cells. The authors des-
cribe the normal cytologic aspect of the Cultures nddi-
cuss certain cytologic problems linked to the development
of cells in cell cultures.
34. IJADERCA, M.IANCONESCU, M.NACHTIGAL. Susceptibility
of humanbr o t sini.~ed~ell~`;~':;3~3d~
ReV;SOt Med'., ' 9398.
The human embryo, a readily obtainable material,
prepared by trypsinization, has a high cellular output per
gram tissue and develops rapidly. Human cambryo cell cul-
tures are extremely favourable i. the growth of poliomyelitis,
Coxsackie, herpes, vaccin.a viruses and adenoviruses, yield-
ing a constant cytopathic effect,
35. N.CAJAL, O.BURDUCEA, S.MATEESCU, G.MARINESCU, MTCEPLEA-
NU, Y.COPELOVICI, The evolution of some experiment-
al viral diseases Wender the action.. of tadioactive
Phosphorus and iodine ( P and-1131). Report
read at the second'Congress of Hungarian-micro-
biologists,. Budapest, September 22-25, 19590
The morbidity and mortality of animals infected with
various viruses (influenza, Coxsackie, poliomyelitis, rabies
and herpes viruses) and submited to a treatment with radio-
11
active isotopes ( P32 and I ) is clearly increased and the
average perio,i of incubation j.d survival reduced in compa-
rison with controls. Biologic titration of the presence of
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
21
the respective viruses showed a more intense multiplication
of the germs in the organism of the irradiated animals.
Under the action of beta and gamma radiations,
some viruses,such as the poliomyelitis virus, type II (MEF1),
may induce experimental infection following inoculation by
routes (peritoneal or subcutaneous) other than those usually
employed. The Coxsackie virus (A2), pathogenic only for the
new-born mouse, determines in the adult animal under the
action of P32 or 1131 a lethal paralytic disease. Moreover,
under the action of internal irradiation, the virus persists
in various organs and tissues a longer period of time than
in the control animals.
36. M.IANCONESCU, I.DERCA. Susceptibility of human embryo
cell cultureA obtained by trypsinization, to infec-
tion with certain virusese St.cerceinframicrobiol.,
microbiol.,"parazitoi., 1959, lo, 2,193.
Human fibroblast;: cell cultures obtained by the
trypsinization technique previously described by the authors,
were inoculated with a great variety of viruses, in view of
studying the cytopathogenic?ef-fect. The following viruses
were tested : poliomyelitis type I. II and III, ECHO virus
type 1-3, 5-9, 11-13 and 19, A.P.C. virus 1-k and 6, herpes
virus, influenza, epidemic mumps, rabies, swine pest viruses,
obtaining a cytopathogenic effect with all the types of polio-
myelitis, ECHO, A.P.C. and herpes viruses. The morphology
of cellular degeneration is different and characteristic for
each group of viruses.
No cytopathogenic effect is obtained with the
rabies, influenza, mumps and swine-pest viruses.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
22
37. M.IANCONESCU, I.ADERCA, N.CAJAL. Cytopathologic modi-
fications produced by -viruses .r " all cultures:
ConsfAtuirea interregionala de "'morfo2agge^,^
Tg.Murept Jan.18-19, 1959.
The authors study the various c;-,.topathologic
aspects caused by the poliomyeli-;-is, herpes, A.P.C. viruses,
etc. in human embryo and amniotic cell cultures, obtained
by tr*psinization.
They discuss the specificity of the lesions pro-
duced by various viruses on "in vitro" cultivated cells and
the possibility of identifying them with a view to making
a diagnosis based on the cytopathogenic aspect.
The investigators likewise discuss the problem
of the parallelism between in vivo and in vitro cellular
lesions.
38. S.MATEESCU. A new type of sterilisable lyophilization
apparatus...with'a vac uffi deutce $t:~e ~ "Infra=
miorobiol 1959.s-109-1:'1- 357 n
A new type of lyophilization apparatus is des-
cribed, sterilisable, economic, readily built.in any labo-
ratory and easily handled, with a vacu= device.
The apparatus comprises two parts : a double wall
glass vessel to which the lyophilization viale are attached
and an empty, 3o to 4o liters water-tight closed container.
A vacuum rubber tube links the vessel and the container to.
a vacuum pump. The vacuum pump operates only 3o minutes at
the beginning of'the process of lyophilization.
Drying of the biopreparations lasts 30 minutes to
2 hours.
Experiments were carried out with this apparatus
during 4 years. The lyophilized preparations obtained were
excellent qualitatively and quantitatively.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
23
39. R..fORTOCALA, S.DUMITRESCU, N.I.IONESCU. Simplified
washing technique of the preparations for
electron microscopy by dialysis. Comunicarile
Acad,R.P.R. Sect.St.Med., 1959,,2, 11,1205.
The authors developee.-. a washing technique of the
preparations for electron microscopy by dialysis. The grids,
covered by a formvar membrane, are put in a Petri dish, on
moist filter paper. A drop of the suspension to be exa-
win@-a.- .s put on this grid and washing is performed by diaj.. /1,U
_InL (3
distilled water soaking the filter paper.
The essential condition for good washing of the
preparation is perfect adherence of. the grid to the moist
filter paper.
This technique has the advantage that it does
not lower the virus content of the suspensions and achieves
a correct washing of the preparations by the removal of
electrolytes from the suspension to be examined.
40. R.PORTOCALA, N..I.IONESCU* M.ANDREESCU, S.DUMITRESCU.
Use of suiphuretted - latin^!ur and palladium in
the shading of preparations for the electron
microscope. Comunicarile Acad..R.P.R. Sect.St.Med.,
19599 29 11, 12o9,
A mixture of sulphuretted platinum and palladium
(at a ratio of 3:1 for the respective methods), readily
evaporates and.can thus be used in the current technique
of shadowing of the preparations foh the electron microscope,
obtaining a sufficient contrast even for E;.:L,yer of 6 A thicYr c4
ness.
The diffraction electron images showed that after
electronic bombardement during 3o minutes at 8o KV, the
layer deposited maintains its amorphous structure.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
24
41. R.PORTOCALA, V.BOERU0 Biochemical methods of inyesti?
ga?hion in electrophoresis micromethods on agar?agar
Selo Voprdsi meditinskoi himii, 1959, .~, 3100
Electrophoretic micromethod for determination of
serum proteins in thin agar films on glass slides. The
electrophoretic curves coincide with those obtained by the
usual electrophoretic methods. It is possible to separate
very small amounts of protein fractions from the serum in
a short interval of time, and then to stain them with bromm
phenol blue.
Quantitative electrophoresis determination is
obtained by means of an automatic densometric system.
42. N?CAJAL. Recent advances in virology. Microbiol., pa-
razitolo, epidemiol., 1959, 4, 293.
The latest advances in the field of virology are
presented, particular attention being paid to morphology ,
biology, the methods for, isolation of viruses in cell cultures,
diagnosis and prophylaxis of the most important viral diseases.
The role of nucleic acids in the reproduction of
viruses, controlled variability especially by recombination
of ribonucleic acid and 'viral protein, host-cell virus rela-
tionship, radioisotope labelling of viruses, the discovery
of ECHO and AoP0C. viruses, the relations between viruses and
tumors, the viral etiology of leukemia., the effect of ioniz-
ing radiations on viruses, new vaccines with live viruses,
efficient in poliomyelitis, measles, etc., are viewed and
analysed in the light of the latest investigations in our
country and abroadd
With a view to recent advances in virology, the
prospects of this specialty are discussed and the important
place it must occupy in biology.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
25-
43-- I0SABUEL. The origin of viruses in the light of dialectic-
materialism. Stud.cerc.inframicrobiol.196o, 11, 2,
307.
Various theories on the origin of viruses are re-
viewed in the light of dialectic materialism and the idealist
theories concerning this question are combated. To conclude,
stress is laid on the discovery of the role of nucleic acids
which raises new aspects of the problem of the origin of vi-
ruses.
44. R0PORTOCALA. The relationship between the immunogenic
activity of an antiviral vaccine and its infecti-
vity. Stud0 cerc . inframicrobiol 0 ,196o, ll, 3, 3650
The role of ribonucleic acid in the viruses that
constitute antiviral vaccines is discusse d0
The conclusion is reached that active vaccination
can be obtained only with a virus that has maintained to a
great extent its infectivity through its RNA9 capable of
inducing the cell to synthetize the homologous virus. The
viruses that have lost this capacity can no longer induce
intracellular immunogenesis.
This implicitly points to the conclusion that the
site of immunity in the sphere of viral infections is the cello
since only within an integral cell, can RNA exert its inducing
action that leads in the last instance to the generation of
virus, which becomes antigen through its protein.
450 ROPORTOOA.LA. New data and interpretations in the problem.
of viral multi,pli.cation~ Stud.cerc.inframicrobiolo
:Ij6oa I, 49 597.
The work is divided into three parts: the first part
is dedicated to our present knowledge on the role of nucleic
acids and particularly of ribonucleic acid ig the mechanism of
multiplication of small viruses, with a simple chemical struc-
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
26
ture, true nucleoproteins The second part sums up the
research work of the author and his coworkers on the in-
fectivity of RNA extracted from influenza and Newcastle vi-
ruses. The third part deals with the interpretation of cer-
tain notions of i.nframicrobiology, viewed in the light of
the latest discoveries on the role of nucleic acids in viral
particles.
46. N.CAJAL? Some considerations on the diagnosis of human
4TF _41
inframicrobial diseases. Microbiolu,.iarazitole-9
opidefniol m 1960, - 5.,.. 3850
A description and analysis of the present diagnostic
possibilities in human viral diseases. The authors show the
use and classify the methods for the isolation of viruses in
laborators animals, az-mbryonated eggs, cell cultures, describ-
ing the various serologic, histopathologic and allergic me-
thods.
The authors point out the importance of a close
collaboration between clinician, epidemiologist and virolog-
ist, as well as the necessity of organizing a great number of
laboratories, endowed with the equipment and biologic products
for viral diagnosis, and staffed with specialists?
The illustrated by a number of synoptic tables
giving indications for the diagnosis of the most important hu-
man inframicrobial diseases, according to the clinical phase.
47? G?DANIELESCUV- V.BOERU, I?ADERCA, M.IANCONESCU. Glucid
metabolic alterations in the chorioa7allantoic
membranes in .vitro: under the influence :of inter-
feron? Studocercoinframicrobiol. 1961;, 12, l,1o9?
Interferon modifies the cellular metabolism of the
chorio-allaatoic membrane, in the sa:cse of an in ibition of
glucose consumption from the medium. This modification be-
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
2-7
homes detectable after .7 house P ;d 'acz t, .24 hours after
contact between the cell ';and the mod-l,rm I. e t inter-
.feron.
A l o w e r ,8:' 11~;IGl/tt~_ C i S l . A 4 u ~ m p ~ i t k n. ' h a s Uketwiame, ibe.ea b-
served in the membranes ,pf th :fte r-f ,xy-n s- Ac e-upare:d with
the controls ": i axd ~Afte.r,add ithe wires.
etfter 24 and
48 hours.
it .is discussed whe.ather the obses-red bdq 4tion of
glucid .metabolism .-is. not one of the e;nuess of interference or
of virus synthesis inhibition under the :i_afhte:e ,of late
r-
.feron.
M BA888..
70 years :since the discover r of the ,basis Of ,sero-
the:rayys 1a882=1959) . Stud,.:ee.rc ; 4fr crob. ol.:1961,
22,, _L .143...
It is emphasized that the first ,categoril.etametet
on the law' of passive immunity, that stands at 'the bae.is of
serotherapy, was made by Victor Babe:g in 1889,
After showing in detail the stages .qf Y ator ;Babee~ s
laborato2 e.xpe r m_ents up to their. f final anc.ceas ;amd ,appiic ationr
of the results obtained to persons '
seoe_re 1y b~tten by wolves'
Ft the Victor Babe.Q Institute in Bnchar~est fbasi.des the Pas-
teur vaccination - the author sta' es that the *oment ;has come
in which the priority of the discovery made by Qictor Ube,
that stands at the basi of serothera
,ahou::1.d be rzecognised
throughout the worie,., .,k_ee;ping account of the Aunqusst~iosable
hstoric:al _facta recognized moreover by a nwlnber of scientists.
49. .
A.DVICI.
The imctomoete virus. Btud,eerc..infrsmicrobiol.
.wig. .~ia~~e
1961. 12.9 19151:.
The techniques for obtaining ncomi?Y
leyte vi=ark ~%e
described .and the theories concerning the mechanism ,of pro-- '
duction of incomplete elementary bodies are discussed. ~4milarly,,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 ,__,.
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
28
morphologic studies linked tc the presence of the incomplete
virus are presented.
50. R..PORTOCALA I..1ARCULESCU, I..SAMURD. On the materialis.t-
dialeetic interpretation in the problem of'vipal
biosynthesis. Cerc.filozofice 1961, 8, 1, 81,
Starting from the experimental results obtained
concerning the replication of some viruses by inoculation into
the. live.cell of their isolated nucleic acids, the authors
indicate the materialist-dialectic aspects that should be de-
veloped in order to understand the process of viral multipli-
cation.
Different conceptions on the nature of viruses are
.critically analysed and the conclusion is reached that although
the fact that viruses are not living matter has not yet been
demonstrated.-it may be admitted at least as a working hypothesis.
51. R.PORTOCALA, N.I.IOI SCU . The morphologZ of aucle.ic acids..
Stud.cerc.inframicrobiol. 1961, 12, 4 55740
The results of morphologic investigations concerning
nucleic acids extracted from tissues or microO anisms are
reviewed. It. is concluded that morphologic O arch work on
nucleic acids is still in its initial stage;.
52. , ACAD. ST..S..NICODAU. The achievements. o1 .,,the, Institute of
InframicrQbiology in its ten years of , activity.
Stud.cere~rinframicrobiol. 1961, ,,supl. , 15.
The-'report briefly reviews the achievements of the
Institute of Ibframicrobiology in the last ten years : sc.ie;n-
tific works,,. didactic activity, acti'Vity kinked to field
necessities..,,.,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
29
3. R;.RORTOCALA. ntrestigatio;aj in t *: inftioua_r-0ie of
viral. muole. c acid"." Stud.ceras.infram~.- g bio1;1961,
129 supl., 43,-- _,
This, paper reviews the results obtained ' in ' inves-
tigationa- carried out. on the infectivity of ribonucleic acids
extracted from the influenza and Newcastle viruses, and des-
'~,.
o ibonucleic said egtraated.from adenovirus. The assumption
is made of the biosynthesis of .viruses under the ~inf- lue;nce, of
nucleic acids and the role of the latter in the mechanism of
immunization.
54. L.MIRZA. The results of the investigations of the Insti-
tute' of
Tr~fraiffi1Cr8b'o?erSiII~Lhe.,raienf
the -dent dT^aartsat.-Bestem,In-v aA,*^di6eas6s,,3s?hd^
viral I=antty: ata~fl a~ a s ab3A t ~ .
12 _8up; ; ~1T9;'
The author reviews the experiments carried out by
the: Institute of Inframicrobiology of the R.P.,E;Acacmy con-
cerning various aspects of the reactivity of-the-Organise in.
viral diseases, in terms of the state of cortical reactivity.
On the one hand, the reactivity of the .oatganise ,
whose cortical activity has been previously modified by various
procedures, following inoculation of various vites? was in-
vestigated, and on the other, the disturbance in cortical
activity that appear after inoculations without the intervention
of an additional factor acting upon the central nervous system.
55? I.ADERCA, M.IANCOIESCU. The value of human embrryyo cell
cultures for-the
J.2abarat?rie h
ot"'ihfram3Crc~UloTo
$tud. eero infre dcrobiofigl;:-"'3 A:'l~al 127A
-The investigations of the authors on cultures of
human embryo cells obtained by trypsiztion of the entire
1 to 3 months old human embryo, accon to :a technique
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
30
devised by the authors, are _ ,iewed. These -cultures are
susceptible to the cultivation of 51 different virus strains,
- IAo c scusp- --
of which ECHO, polioviruses, etc. The authors
works carried out in the Institute in which hum;a :e~ yo aeil
cultures were used, such as replication of adeao-iirus type 3,
the preparation of interferon, isolation of hepatitis virus.
The theoretical premises that stand at the bazis
of the high biological value of this type of cell culture are
discussed.
56. ACAD.ST.S.NICOZAU, L.MIRZA, I.SAMUEL, T.ENACHE. A study
of the immunogenic-potential of formolized rabies
and influenza- vaaeines b means of the conditioned
reflex method. - Stud eerc:inframicrobiol.1961,
12; supl: 175?
The conditioned reflex method was used as a test
for the determination of the degree of viability of.. the... Y1
ruses contained in a rabies and influenza vaccine ~-tx',~a`ka.
by means of chemical agents. The investigations showed that
addition of formol up to 4% in the case of the rabies virus
and 1 %o in the case of the influenza virus respectively,
does not impair their viability and maintains their immuno.
genic potential as well as their property of disturbing
previously established conditioned reflexes. Addition of
formol over 4 %o or 1 %o in the case of rabies and influen-
za viruses respectively, kills the germs and Mtppresses their
immunogenic potential and their action on conditioned reflexes,
which are not altered.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part -Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
II
VIRUB$8 AND TUMORS
57 - 86
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 `-?-~
31
AC.STeZs1`IIQI,AU. C?11ug roll >rat_~, s in the human
and animal or ani?m . under. the -"influence -of._ certain
inframierobial erma. Some considerations on the
viral-h. othesis of cancer. Lectures. The Publish
ing House of'the Chinese People's Republic Academy
of Sciences, 1954-55, 79?
The author reviews the various proliferative eel-
lular pro'cesse's' induced in the organism by the activity of
inframicrobial germs, referring especially to a number of
17 viruses with the most varied affinities : neurotropic,.
epitheliotropic, mesodermotropic and pantropic. On the basis
of more than 70 bibliographic indications, part of which from
personal worse, new data and concrete arguments are brought
for the understanding of the mechanism of tumoral genesis under
the influence of known inframicrobial factors. Interpretation
based on numerous morphophysiopathologic documents lends
support to the conception of the viral etiology of tumors, a
field of investigation in which Soviet science has carried out
increasingly interesting research-works,.
The problem of the inframic robial etiology of cancer..
seems to near, its final solution due to the remarcable' and
fruitful effort of the Soviet investigators.
paper.
.Many figures (41 figures) illustrate the present
N.CAJAL. Inframicrobial etiology of tumors in the light
-of the latest investigations. Probleme de Infra-
microbiol,, Edit .Med;,Bucuregai, 1956, 19 99.
After describing the-known data in literature on
the viral etiology of certain benign human tumors, and benign
and malignant tumors encountered in animals, new data are
brought sustaining the hypothesis of the viral etiology of
cancer in general,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
32.
These data show tb.- intervention of a specific
virus, which penetrates into the organism already during
the first days of life (probably with the mothers milk),
in the production of malignant tumors. This virue is
maintained for a long time in a state of equilibrium in the
organism in a biophyt state.
Following an upset of the equilibrium betveen the
inframicro-organism and the macro-organism, a situation to
which numerous factors can give rise but especially neuro-
endocrine modifications, the virus regains its virulence,
multiplies abundantly and enters into conflict with the
organism. When the organism manages to overcome the germ
by defense reactions, it becomes sterile as regards the
virus; in the contrary case, the inframicrobe develops
actively and furthers its oncogenic action which results in
the appearance of cancer.
59. ACAD.ST.S.NICODAU, N.CAJAL. Considerations on the
viral. etiology of cancer. St.cerc.inframicro-
biol.,mierobiol. , parazitol., 1957, 8, 4, 493?
After reviewing the last investigations regarding
the viral origin of cancer, and describing their point of
view, the authors uphold a personal theory on the mechanism
of appearance of malignant tumors under the action of an
inframicrobe. The hypothesis of, a complex mechanism is
emitted, according to which an important part in the appear-
ance and.evolution of cancer is played not only by the virus
that penetrates at an early date into the organism and per-
sists for a long time in a biophyt statetbut also by the
endocrine and nervous systems.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
6e. E.NASTAG. The action of some viruses on experiment
tumors in mice. and rats. St.cerc.inframicrobiol.,
mierobiol.,parazitol., 1958,- , 2, 213.
A.study was carried out concerning the action of
the fowl plague virus, strain 863, adapted to mice, on mouse
spontaneous sarcoma 97. The virus does not modify the cli-
nical evolution of the tumor. Inoculated to epithelioma
M I bearing mice, the virue' sups ressea the graft ng faculty
of the tumor, which develops slower than the controls.
The same virus influences rat methyleholanthrene
and spontaneous sarcoma, determining resorption or necrosis,
with partial or'total elimination of the tumoral tissue,
suppression of the. grafting faculty at the 3rd serial pas-
sage and prolongation of the incubation period of the tumor.
The virus isolated from the tumor or from the brain
of the infected animal is much less virulent thtLn the original
strain,
Rat spontaneous sarcoma is likewise- influenced in
its evolution by the swine plague virus inoculated into the
general circulation of the animal, but is not influenced by
the parapoliomyelitis MM virus. Ebidemic parotiditis virus,
and to a lesser extent ectromelia virus, exercises an evi-
dent lytic action on this tumor.
61. E'.NASTAC, h,SARATEAJU. Study of the action of the virus on Ehrlich-ascitic carcinoma. St.cerc.in-
framicrobiol..,microbiol.,parazitol. ,1958, , 4,421.
The mumps virus inoculated in situ exercises on
the tumor a temporary destructive effect, manifested by the
higher percentage of degenerative cells and by the lower
number of cancerous cells per cubic mm..in the groups which
received the inoculum simultaneously 48 and 72 hours after
the graft.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
The mumps virus does not influence the grafting
faculty of the tumor until the third passage and does not
cultivate in it0
P-o E.NASTAC 0 B.FUHRER. Research-work regarding the action
of certain virusea. on Lhrlich ascitic carainomao
Action of the hexes virus and vaccinia viruso
Sesiunea Tinere tului Accad0R.P0R. ,Avril 25 0 1958 ?
The herpes virus inoculated in situ does not
influence the transmissiMlity of ascitie carcinoma in mice,
inowalated on the 4th day after the tumoral graft, It in-
fluences however the morphology of the tumoral cell,"inhibit
ing tt,, multiplication process at the first passage.
The vaccinia virus inoculated on the 4th and 6th
day after the graft, prevents the development of the tumor
t the first passage, a fact which is also confirmed by the
morphologic aspect of the tumoral cell.
Neither the vaccinia virus nor the herpes virus
can be recovered from the tumor.,.
65. EoNASTAC. Effect of the mumps virus on rat spontaneous
sarcoma Comunicarile Acad?R.P?R., 1958, 8, ll,
12o7. Rev.8ci,Med., 19590 4v 690
The mumps virus in allantoic fluid, taken from
developing chick embryos infected with this virus, exercise
a lytic action on the spontaneous sarcoma of the rat when
it is inoculated intravenously to this animal,
The oncolytie action may be detected clinically
and ontologically.
The virus does not influence however the grafting
faculty at the first passage.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
35
64o N?CAJAL, E.NASTAC. Study of the development of the
G u e r i n .yitheliena in rats Pr9Ti-GR@A:Lr infected
With- the IM44 is vi u
~Bq Staeersoimf am3arobiolfl q
1,959, 9 4g-4 14 L? R4 oSoAohI0q,oq~
The evolution of the Guerin 1'8 epithelioma grafted
on previously immunized rats is studied?
The grafts made on animals immunized 2o and 4o days
previously9, give a higher rate of positive results, the *u ,.ors
appearing soom.,r and their development being greater thanI
the control animals,
Sixty and especially eighty days after immuni-
zation, the. tumor grafts gave a lower percentage of positive
results, the tumors appeared later and they developed less
than in the control animals.
The .grafting faculty and the morphologic substrate
are not influenced by the development of the Guerin T8 epithel-
ioma in rats previously immunized to the vaccinia virus.
65. O?COSTACHEL9 EJNASTAC, BAILIE. The influence of the vacci:=
nia virus associated to narcosis on t4.e evolution
of Guerin T8- a ithelioa;iaa ice.-..rats.
fr .ierobiolo,~..1959'~..l,o 4a-42,50
Stacercoiu?
. . The vaccinia virus inoculated by intraveoc
or intraperitoneal route to rats with Guerin caarcinow in-
hibits tumoral growth at the beginning of its develop
Deep and prolonged narcosis during 45 urs,
associated to-the vaccinia virus, decrease the pro ion
of metastases and the mortality rate, as compared the
control group and to the group inoculated only with the
vaccinia virus.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
36
66. A.AEREVI3I, AL.PETRESOU. The action of the hydrosoluble
fraction of bee royal ' jelly on mouse8 -
aseitogenie tumor: C:R:dea"Sacaa"fle"Ta~Soc.Biol.,
1959, 1539 2, 172o.
The hydrosoluble extract of royal jelly produced
y Apis mellifica exercises an oncolytic effect on the Ehrlich
ascitic tumor, inoculated to mice by intraperitoneal roate.
This effect is proportional to the amount of the
product used.and to the interval of time after infection,
maximum changes being observed on the loth day after ino-
culation,
Oncolytic changes are to be found also in smears
from the peritoneal fluid of the second passage,.in which
the above mentioned product was not used.
67. E,NA TAO, B.FUHRER. Research-work regarding the action
of certain viruses on Ehrlich- aseitie eareinOmaa
Action of the lymphooytic'. ehorio=~me:ningitis" virus
and the MM Virus*"*- -Comtic&rile.. Aead;R.P.R; 1959;
9, 6, 64.5.
The MM virus destroys and prevents multiplication
of the tumoral cells and suppresses the grafting faculty
of the tumor when inoculated in situ 24 hours or 4 days after
grafting. The chorio-meningitis virus does not influence
transmissibility of the tumor.
The morphologic study of the tumoral cells shows
the destructive action exercised by the MM virus and the ab-
sence of this action with the ahorio-meningitis virus.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
37
68. E.NASTAC, B.FUMRBR, d.SAMUEi. Reproduction of the Ehrlich.
ascitie- carcinoma by acellular filtr_ St0cerc,
inframicrobiol.,microbiol,,parazitol., 1959, lo,
1, 89. m Rev.Sci?Med., 1959, 4, 730
Inoculation by intraperitoneal route of an acela
lular filtrate of the Ehrlich tumor reproduces the tumor in
a clinically inapparent form. The amount of ascitis producqjj
is much amaller and the evolutjam of the tumor much slower than
that of the control tumor obtained by inoculation of total
ascitis by the same route, The tumor obtained by acellular
filtrate can be serially pasmaged.. The etiologic agent of the
Ehrlich tumor seems to be a filtrable germ,
69. B,NASTAG, B,Ft R. Action of the herpes virus on the
Croocker sarcoma in mice, St,cerc.inframicrobiol,,
1959, 10. 217;3
The herpes virus inoculated subcutaneously together
with a tumoral suspension, stimulates the development of the
tumor as compared to controls,
The herpes virus was not recoverable 2 weeks after
grafting, neither from the tumor nor from the brain of the
animals with infected tumors. It is possible however that
it should exist in various tissues in a "masked" forma
7o. E.NASTAC, B.FUHRER, D,SARATEANU. Research-work regarding
the action of certain viruses on Ehrlich ascitic
carcinoma in mice. R4v.Sci.M4d., 1959, 1,770
The viruses of epidemic mumps, parapoliomyelitis
MM, vaccinia and herpes, inoculated by intraperitoneal route
to mice with Ehrlich ascitic carcinoma exercise a partial or
total destructive effect on the carcinomatous cells,
The lymphocytic chorio-meningitis virus inoculated
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
38
in the same cond.itions.did not show the same properties.
71. O.COSTACHEL, B.NASTAC, B.ILIB. The action of ovovaccine
associated to cytostatics on the evolution of
Gu4rin tdaer in . rats. St.cerc.inframicrobiol.,
196o, 119. 1, 31.
Potentiation of the action of certain cytostaties
(Degranol, Sarcolysin) on Cu4rin tumor with the aid of
the"$accinia virus was attempted. The following results were
obtained : .
Ovovaccinia inoculated concomitantly to grafting
of the tumor intensely stimulates successful grafting and
growth of the graft ; o This virus inoculated on the 3rd week
after grafting inhibits the small tumors-and stimulate the
growth of large tumors.
The interval between inoculation of.the virus and
of the cytostatics is determinate : ;;motion of the ovo-
vaccinia-Degranol association on Guerin tumor.
The action of association of virus and cytostatics
on the tumor show-Ah&t the trophic modifications produced by
the viral infection have a marked influence on the action
.of the cytostatics.
72. B.NASTAC, B.FUHRBR. Investigations concerning the action
of certain viruses on some experimental tumors;,
Stud.cerc.inframicrobiol.,196o, ll 89 and Acta
virol.Hung.196o, Z. 2, 181.
Using seven virus strains, the following results
were obtained
The'avian pest virus, the neurotropic strain 836
adapted to mice, suppresses the grafting faculty of sarcoma
97 in mice and prolongs the life of the cancerous animals,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
39
when inoculated in situ or in the general circulation. Si-
milarly, it suppresses the grafting faculty and inhibits the
development of mouse epithelioma MI. The same virus inhibits
the development and partially suppresses the grafting fa-
culty of rat methylcholanthren sarcoma. It induces partial
or total oncolysis of spontaneous sarcoma, protracting the
period of incubation and suppressing the grafting faculty
at the 3rd serial passage. Inoculated into the organism of
the cancerous animal, the avian pest virus loses its viru-
lence to a certain extent.
The mumps virus exercises an oncolytic action on
rat spontaneous sarcoma and a temporary destructive action
on Ehrlich ascites carcinoma. Associated to the vaccinia
virus it does not maintain its destructive effect on Ehrlich
ascites carcinoma.
The encephalo-myocarditis MM virus does not
influence the evolution of rat spontaneous sarcoma, but pre-
vents the multiplication and destruction of carcinomatous
cells in Ehrlich ascites in mice, suppressing the grafting
faculty of this tumor.
The ectromelia virus determines intense necrosis
and elimination of the cancerous tissue in spontaneous sar-
coma bearing rats.
The herpes virus, inoculated in situ arrests the
multiplication process of cancerous cells in mouse Ehrlich
ascites carcinomatous tumor. In mouse Crooker tumor, sti-
mulation of the development of'the tumor is observed after
inoculation of a mixture of tumoral and herpetic suspensions.
Influence of the vaocinia virus on Ehrlich as-
cites carcinoma is manifested by suppression of the grafting
faculty and destruction of the tumoral cells when inoculated
4 to 6 days after grafting. Inoculated to epithelioma T8
bearing rats it has an oncolytic action that is enhanced by
deep narcosis of 4-5 hours and association of o"9.1.eACadOA--re ?
1 t 8 9 8950.....,
The e~'ierogenous hepatitis virus, examined in the
electron microscope, appears in most cases W when the germs are
isolated m in the form of more or less regular, small spheruleso
Their average size is 9o o loo Mpg maximum size
10 m~ and minimum size 8o M/4 -
When the germs are ranged in twoes or threes,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
57
they appear in a flat discoidal forme recalling up to a cer-
tain extent the aspect of red blood cells grouped together
like a pile of coins. The size of the sclerogenous hepatitis
virus is approximately twofold smaller than that of the Botki,n
hepatitis virus
These data constitute an additional argument con-
firming the previously established concept of the plurality
on inframicrobial epidemic hepatitidesE,
lo3a R0PORT00ALA. Investigations on epidemic hepatitides,
Report read at the inauguration of the Institute
of Inframicrobiology (July 12, 1951,)
A brief description of the contribution of the
Rumanian School of Inframicrobiology to the study of epidemic
hepatitis,
These investigations resulted in identification
of the hepatitis epidemic which broke out in our country
before 19489 with Botki:n&s hepatitis; establishment of the
viral nature of this di.4ease by histopathologic studies,
the finding of specific inclusionse experiments on laboratory
animals and, detection of inframicrobes; establishment of
histologic diagnosis oriterions based on the presence of
triad (I = intranuclear inclusions 9 N = giant
K = multiple karyokinesis); listing of catarrhal
icterus together with Botkin?s hepatitis; determination of
conditions in which epidemic" outbreaks appear- detection
of cases of unapparent disease; data regarding the trans-
mission of the disease to animals.
In sclerogeniv 'epatitis the following were es-
tablished :'the histo J. thoiogic appertenance 9 which defini-
tely differs from that of Botkin's disease; clinical dif-
ferences between the two hepatitides; application of the
hemagglutination reaction; certain properties of the virus;
study of peripheral blood; determination of nucleic acids
in the sera of the patients.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
58
104. NOCAJ'AL, S.MATEESCU m .w , - tSS.IFVICI 9 G0POPESCU, M.CEPLEA-
NU, R0CAPRARU. Distribution of the sclerogenous
epidemic hepatitis virus in the human body.
St.cerc.inframicrobiolo,microbiol.,parazitol.,
1952, 39 1-2, 1010
B4 means of the hemagglutination and hemagglutina-
tion=inhibition tests-, it was possible to demonstrate that
the scierogenous epidemic hepatitis virus is to be found
constantly in high concentrations in the liver, duodenum,
pancreas and kidneys and in smaller amounts in the jejuno-Q
ileon0
The virus was not constantly found in the stomach,
large intestine, suprarenal capsules, spleen, lungs, brain,
cerebellum and spinal cord ; it is usually absent in the
~- I~lfl d.esti?les and lymph nodese
The sclerogenous epidemic hepatitis virus is there-
fore a virus with viscerotropic affinities, but can in cer-
tain cases be found in ectodermal tissues, having therefore
also pantropic possibilities.
105. ACAD.STeS.NICOLLU, N.CAJAL, C.ALBU, M.CEPLEANU
Cultivation of aclerogenous epidemic hepatitis
virus in embryoonated eggs. St.cere.inframicro-d
biol.,microbiol.,parazitol., 1952, 3, 1-2, 29.
Inoculation of the sclerogeuous inframicrobial
epidemic hepatitis virus on the chorio-allantoic membrane,.
yolk and amniotic sacs of the chick embryo, induces after
the third passage, constant lesions in the embryo, which
however lives until hatching.
The most interesting lesions are to be found in
the liver. They become apparent three days after inoculation
and become more severe as the moment of hatching approaches.
Only after inoculation, inconstant, congestive, infiltrative
and proliferative lesions can be observed on the chorio-
allantoic membrane.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
59
After the 9th passage with antigens from the liver
of embryos and from the whole embryo9 positive homagglutination
reactions are obtained, inhibited by the serum of convalescents
of sc.lerogenous inframicrobial epidemic hepatitis.
.lob. ACAD.ST0S,NICOIAAU, C.ZAMFIR, NOCAJAL, GOBALMUS, M.SEPEA-
1W-FIRICA; -S.MATEESCU, E.TURCU; I.NICOLAESCU.
"Legiofls. of-the"liver in inframidrobial sclerogenous
_1112: p~tSt2~o13t~TeStS~.StaA~ad:R4P:R. Sect.
StoMed;"3932s,4;^3; 105.-
In the course of the study of a new type of viral
hepatitis, i.e. inframicrobial sclerogenous epidemic hepatitis
identified in the R.P.R. in 1949, the histopathologic modifi-
cations induced by this virus at the level of, the liver were
described. Investigations were carried out on 63 cases; 44
biopsies were performed according to the Roholm Iversen
method and 19 necropsies; these examinations , were carried
out at various stages, beginning from the first week of the
disease until the 28th,
On the paraffin embeded liver sections, stained by
various methods, it was possible to detect, in the first phases
of the disease, the presence of massive infiltrations with
prevalent polynuclear and mononuclear elements; in a more
advanced stage, the mononuclears became predominant. However,
in this case, when the infiltration on all the sections is
characterized by mononuclear cells, there may exist zones of
probably recent tissular infection, which take on the aspect
of acute inflamation with predominant polynuclear leukocytes.
Moreover, during the initial period of the disease, marked
hyperemic modifications can be observed, contrasting strong-
ly with the images encountered in Botkin?s inframicrobial
hepatitis. The investigations of the authors likewise show
that sclerogenous hepatitis is fundamentally different from
the latter. The processes of multiplicatiom,,4haracterizeA in
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
6o -
Botkin's hepatitis by num roes karyokineses, are extremely
rare in this viral hepatitis, in which pOeu.do-giant cells,
that appear after swift amitotic multiplication, are pre-
valent. Nuclear inclusions were not detected either in
this stage or in a more advanced stage of the disease.
The general aspect of the liver suffers greater
modifications with the evolution of the disease. The glan-
dular tissue has a tendency to be replaced partly or wholly
in certain areas by a juvenile connective tissue. In severe
cases,. with a lethal end, the sclerous neoformation'tissue
may replace up to 3/4 of the surface of the section examined.
Numerous newly formed bile canaliculi furrow this
tissue whose hepatic origin is unrecognizable. Certain cells
in the microscopic field show varied degenerescence (vacuolar
aspect, necrobiosis with picnotic nucleus, achromatic dege-
nerescence). The-hepatic cell often completely loses its
characteristic morphologic aspect, presenting the aspect of
a ghost cell.
1o7. ACAD.ST.S.NICOLAU, NOCAJAL. The latest investigations in
1 the Rumanian People's Republic on viral epidemic hepa-
titidesa Rev.St.Med.' Med. Intern6 -1952a-4s-1 12-
Data regarding the investigations carried out in
the R0P0Ro on the hepatitis epidemic, that started in 1948,
and which revealed the plurality of viral epidemic hepatitides.
A description is given, particularly of the results obtained
following morphologic study in the electron microscope, of
the properties of the virus, the histopathologic aspect, the
clinical and hematologic picture of sclerogenous hepatitis.
,Thus, the hemagglutinating-property, the action
of certain physical and chemical agents on the sclerogenous
virus, its cultivation and the alterations produced in the
embryonated egg, the distribution of the virus in the human
organism, hepatic lesions in the course of the natural disease,
the clinical and epidem.iologic characters of sclerogenous he-
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
61
patitis, and the hemommedullogram aspects are dealt with in
detailo
The data obtained following the latest investiga-
tions in the RbP?R?. establish the characteristics of sclero-
genous hepatitis and differentiate it from the other known
hepatitides, thus lending support to the plurality of viral
epidemic hepati.tidese
lo8? 'ACADOST?S,NICOLAU, N?CAJAL? Etiologic ? histopathologic
and itmunologic data in' epidemic viral-he
Pa .Per read'at the-"Rumanian Soviet-Medical D s"
17 April 19524
A presentation of the known data in literature,
starting with the year 1885 when Botkin emitted the hypothesis
of the infectious etiology of catarrhal icterus, with special
stress on the etiology, histopathology and immunology of the
disease,
The classical findings are completed with the re-
sults obtained by the authork, which evidence the plurality
of viral epidemic hepatitideb? A description is given of the
characteristics of Notkin's hepatitis and especially of scle-
rogenous hepatitis,-a nosologic entity recently identified in
the ROPOR? Stress is laid on the differences that reside
particularly in the histopathologic picture (the presence of
the inclusion-analeole-karyokinesi,s triad in Botkin' s hepa-
titis; the absence of inclusions and abundent proliferation
of connective tissue in sclerogenous hepatitis) and on the
a .sense of cross immunity?
Data regarding the properties of the virus and
experimentation are likewise expounded, special mention
being made of the results obtained by the authors in their
investigations&
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
62
109. N.CAJALfl S.MATLRSCU, G.POPESCU. LL.LISSIEVICI, M.CEPLEA-
- Det a obtained by means of the hemaggl utino-
inhibition test in' epidemmie " sclerogenous hepa
e.~r.~.rir ieca
titiso St?core.inframierObiel;miCrcibial.,pa-
raZitol., 1953, 4, 1-2, 67.
The hemagglutino-inhibition test with the serum
of hepatitis patients (1,oo4 cases), using as antigen liver
emulsions from a man who died of this viral disease, was
positive in 81.38% of the cases studied during the height of
the disease. A titer higher than 1/4o is considered positive.
A--number of 238 tests Were performed with the
sera of persons in contact with the hepatitis patients,
yielding 428% positive results. In 382 healthy persons,
positive res!)'Its were found in 12.82% of the cases, which
is accounted for by the occult immunizations in the course
of the clinically apparent disease. Hemagglutino-inhibit-
ing antibodies were experimentally revealed in guinea pigs
and rabbits, inoculated with the hepatitis virus.
llo. N.CAJAL, EL?LISSIEVICI, S.MATEESCU, G.POPESOU, Y.COPE-
LOVICI,'RoCAPRARU. Duration of elimination of
the 'epidemic- hepatitis virus - in patients and.
coivalesef~ts~, -8t;eeio:inframicrobiol.,micro-
biol.,para2itol., 19539.4o 3??4, 2'71?
The duration of virus elimination in 445 pa-
tients and convalescents of epidemic hepatitis was studied.
The presence of the virus was tested in the feces and urine
of 219 patients, only in the, urine of llo patients and in
the naso-pharyngeal washings of 116 patients. The examina-
tions were repeated several times in 51 patients, in order
to establish the evolution of the specific elimination of
the virus9'
The hemagglutination and hemagglutination-inhi-
bition reactions were performed with centrifuged emulsions
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
_63
off{,Bg~,rne^_and,`nasopharyn_geal washings asanetgens;
lPor,~theehemaggl,ut nat on-inhibition te's:t.s.tandnrd anti _Rotk n
wand axiti sclerogeriottsf1serra tweje)gusedo
The investigations carried out showed that the
' l .. ..Y-- - \t .n++.', t- 'i!f a. _. .>.lfl' as y}R>-,O Mr .- (?
hepatitis-;virus is~ constantly,--elim hated through the feces,
more seldom through the urine. and only exceptionally in the
xiasoApha ^ n@;ea~l~ se'eret3"ons'od `Eid? nation' of --the, virus may
`take? pl=acedruring`the 4diseas?'e?:up~ toy a'ive'~moment= mot's or
Tess? diet tit"from- he moment oaf acl`iri c'al recover a ~n 'c"er'tain
cases the vibrus,`~diDi' st lwl ?be e1 m3Hated a ter' ht s1- e riod
(acco di'rg rto -t'he I inver - igati'ons` of' 'the f'aizthors` tip "to
255
days). Flimin'ation? of th*evirus after' "cliniea`l~ r'r.
=r`e.c owe
' ` -demdnstraties'tatar the' patirei%tcondred as healed, cos
" ':trues' Yto? shave '?iHbux"" s f -1 Re f?ort
hepatitis.
.ogic alterations inhuman embryo cell culC;sres obts3nfd by
read at the Interreg. iO il-:.C.6SLferefic6'o>Q !epidemic
hepatLt~ia.:c8inaia,: Oct?ber X21=P2 19;60..
A .study was carried out on the ![dtol strafii," the
assumed .agent of epidemic hepatitisj, isolated by .Kulaelka in
Czechoslovakia' from the viewpoint of its' cultivation.in va-
rious cell cultures, laboratory animals and embryonated egga.
The authors describe the characters of the m64ho-
trypsinizaton'and'in the organs of inoculated @riimals.
-1770 ACAD:ST.S.IJICOLAU; '`N.CAJAL.
t1oz s - ?r'th8' ine`tftute' O:
micr'o'bial e de' c e& it s
o`l 'r9: ew i . , AsaP14. y :...
Mhe, !results~ ,.,obatained, tby the . investigators of the
. stit.ute of Infrramicrob.iology An the field of viral.,Ipl(eaic
h:patitis during the 1.949-1961? period are ,described.
S O.ZAVATF . N:CONSTANTINNSCU.. N..BIRZUj, C.OANA, GH..PANAI-
TFBCU, AL.MIHAIL. Infectious virus henat~? i 8'
EI:V.?H) within ,the _.:faaily env. .ronme:nt...8tud
The familial .incidence of h .V J. bavas `" rives3t gated
over r-a period of six years `(953-1958) three towns' of about
25o.ooo inhabitants each, situated in different geographical
areas of the, R.?-R.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 CIA-RDP80T00246A019200640001-8
- 1o6
Out of 5,468'families in which I.V.H. oeeured,,
the disease was confirmed to a single subject in 95 per cent
of the cases. In 1.8 per cent or the iaruilies, tine secondary
cases occured at intervals ~ of o-6o days following the first
case'while in 3,2 per cent of the families several cases
occured at long intervals ranging from two months to five
years.
In 151 families in which secondary cases appeared
within o-16o days, 74 per cent of these cases involved children,,
-.:the possible intra-familial source . of infection being an ini-
tial::..case concerning another child. In contradistinction of
tha::group of families in wich hepatitis exclusively ocoured
among children, in the group in which the disease involved
adult subjects, the incidence rate of families exhibiting se-
condary cases within o-6o days was much lower, i.e, of 18,6,
lo.9 and 16.9 per cent respectively.
higher during the cold season of the year, corresponding to
the.autumn-summer peaks of I.V.H.
A series of general prophylactic measures manly
concerning the o-14 year age-groups as well as the prophylac-
tic use of gammaglobu.lins are required.
The.incidence rate of secondary cases within the family is
The incidence rate of secondary cases among.chil-
res is of 4.8 per cent, as compared to o.6 per cent in adults.
.179 P.ATHANASIU, N.CAJAL, M.IALOMITEANU, M.ANDREESCU, 0.
SFERDIAN. Comparative investigations on liver
and muscle aldolase in epidemic hepatitis sera.
Stud-.cerc, inframicrobi'ol.,1961,' 12 supl.,295
...and Rev.Sci.M4d., 1961, 3o4.
Determinations of fructose-l-monophosphate aldo-
lase and fructose-1.6-diphosphate aldolase in the sera of loo psr-
tients suffering from virus hepatitis revelated the presence of
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80TOO246AO19200640001-8
increased values up to lo-4o u and 2o-8o u respectively, as
compared to o-8 a and lo-2o u respectively in normal subjects
or in those suffering, from other liver diseases.
Since fructose-l-monophosphate aldolase is exclu-
sively formed in the liver, simultaneous performing of both
tests makes it possible to distinguish this enzyme from muscle
aldolase. Nstimation of the two results may yield more com-
plete'informa-tion concerning the diagnosis of virus hepatitis.
l8o N.CAJAL, O,MITROIU, C.BARBU, O.CONSTANDACHR, M.POPA.
Comparative study of hemaarglutinatiou reactions
with` the red cells of MRhesus and of one-da-vi d
chickens in epidemic hepatitis -Stud* cero'`infga
microbiol.,1961,-12, sup1:; 301.
The results are reported of a comparative study
of hemagglutination reactions performed with the sera of
patients' suffering from epidemic' hepatitis or other viral
diseases'an*'the red cells of one-day-old chickens and of
M. Rhesus.
The figures recorded by the present authors in
cases of epidemic hepatitis are lower than those reported by
other investigators, Altough these figures are definitely
increased as compared to those recorded in normal subjects,
Vz.ey cannot be used for diagnostic purposes since similar-
increases occur.in other diseases as well. Hence,' these tests
a ve of little avail for the diagnosis of, epidemic hepatitis as
prevailing at'present in the Rumanian People's Republic.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 : CIA-RDP80T00246AO19200640001-8 .;.~
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
181 ACAI FT NIC T:.AT` STROE G ~SC'J
GR, LAZAR:i;SMT r Herpes and. herpes parae3mmunity induced in man bypoliomye-
litis. The possibility of paraspecific prophylaxis
in poliomyelitis by means of experimental herpe-
tizationa, St.cerc.inframicrobiol., 1950, It 1,
1210
After a review of various cases of paraimmunty
reported in viral diseases, the facts.that point out the
existence of para-immunity between herpes and poliomyelitis
are discussed. An experimental study was carried out, by
inoculations of the herpes virus on the skin of paralytic
poliomyelitis patients and former patients, in order to in-
vestigate if an increased paraimmune resistance to herpes
is built up. Inoculations performed on 55 subjects showed
that starting from the second month after the onset of polio-
myelitis an increased resistance to the herpes virus exists,
the number of positive inoculations decreasing from 72.7 % to
26.1 % in the second month and 4% between the 3rd month and
the 7th year after the poliomyelitic infection. The authors
propose to investigate if herpes is followed by an increase
of resistance to poliomyelitis and if herpetization could
constitute a method for combating poliomyelitis.
182, ACAD.ST..S.NICOLAU, T.HORNETZ, R.DINU, S.MATEESCU, M.SE-
PEANU-FIRICA. A histopathologic study .of acute
anteriorpoliomyelitis;.changes in the peripheral
and central sensitive systems. St.cerc.inframicro-.
biol. microbiol.,parazitol., 1951, 2, 1-4, 131.
Diffuse changes of the glial reaction type, with
neuronophagia, inflammatory infiltration or necrosis, were ob-
served in the spinal ganglia, in the posterior horns of the
spinal cord, in the Goll and Burdach nuclei, in the nucleus
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
109
arcuatus,~'in the locus coeruleus, in the roof nuclei and
cortex of the cerebellum, in the thalamus and in certain zones
of the cerebral-cortex.
183. N-.CAJAL, EL.LISSIEVICI-OPRESCU. Investigations on sego-
neutralizing Poliomyelitis II Clansing) antibodies
in the inhabitants of Bucnerest. Third' European
Symposium of Poliomyelitis, Zurich, 1955, 63.
St.cerc...inframicrobiol.,microbiol.,parazitol.,
19569-2, 1-2t-'570'.
A.'..study on .the, presence of neutralizing antipolio-
myelitis II (Lansing) antibodies in lo8 persons of different
sexes 'and: ages in , BucaareS'y, established that these antibodies
are to be found in a high percentage in new-born infants, dis-
appear. at the =age of". 6-12 months, after which incidence, `in-
creases, attaining a~maximum titer at the age of 2o-3o,.after,
which it dec"reaies `again,
It was not possible however`. to define the;'nature
of these antibodies ; they are either type or group specific
or non-specific - paraimmune.- The investigations carried out
showed however that a close coitmelatjon can be established
between the presence of these. antibodies and receptivity to,
poliomyelitis, the morbidity rate being proportional to the
number of negative results obtained'in each aige-group.
184; N.CAJAL, N.DRAGANESCU. The action of hargactyl in
mouse experimental poliomyelitis with virus II
(Lansi)..Lucrarile cele de a II:a Sesiuni gtiin-
Vifice a IO?F,, Buc., 13=-15 aprilie 1956, 9 ,
A study was carried out in 3 experiments on 84 mice
inoculated by cerebral route.with poliomyelitis virus' 'type
II (ansing) on the evolution of the experimental disease un-
der the.action.of Largactyl, administered daily by intravenous
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
110
route, starting from the day after infection (5 . mgr ,per
kilogram body weight).
The experiments showed that, in comparison-with
the controls in which mortality was loo%, mortality was rela-
tively low (85%) in the treated animals and incubation longer
(14 days as compared to lo days in the controls). The diffe-
rence.was more evident in the groups inoculated uitj 10-2
virus dilutions.
185. G.MARINESCU, N.DRAGANESCU, I.TURCU, I.FRIDMAN,VCIUREZU,
P.ROMAN. Interstitial . pneumonia. in 'poliomyelitis.
Presse Med., 1956, 64, 94, 2207.
The present paper deals with 62 cases of polio-
myelitis, most of which with ascending forms, followed .up
during the last 6 years. The macroscopic examination showed:'
19 broncho-pneumonia, 22 marked congestions, 5 pleuro-pulmonary
hemorrhagies, 1 pulmonary collapse, 1 massive emphysema,
1 pulmonary tuberculosis.
The microscopic examination revealed broncho -
pneumonic foci in 11 cases, typical interstitial pneumonia An
to cases (3o%), mixed pneumonia (associated interstitial
pneumonia) in 7 cases, ateleotasis processes in 3 casesand
hemorrhagic processes in 2 cases out of the 33 cases examined.
The interstitial pneumonias were more frequent and wide-spread
in small children who died during the first week of the disease,
although the most typical lymphocyte infiltrations were to-be
found in children with a longer period of survival. Rich in-
filtrations were likewise found in the cases with more intense
interstitial myocarditis and processes of 'perivascularitis in
.the nervous system.
These infiltrations seem to be due to the polio-
myelitis virus, either because of its particular viscerotropism
or its presence in the blood during the first - part - of `the'.di-
sease. The action of the regurgitated gastric fluid and aspired
?bucco-pharyngeal secretions may also (be:considered:as,.adjuvant
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
lii
factors. Stress is laid -on the importance of interstitial
pneumonia as an aggravating factor in evolutive poliomye-
litis.
186.. N.CAJAL. Present stage in anti.. olio elitis vaccinatio ,
"Viaita Medical." 1957, 3, 31.0
This paper is a brief survey of our general know-
ledge on the protection and efficacy of the Salk vaccine. Stress
is laid on the necessity of continuing investigations in view
of improving this first effective vaccine, since the protection
conferred by this vaccine with an inactivated virus is not
complete. In view of the results obtained by,various investi-
gators, in experiments on live, modified poliomyelitis viruses,
.and the mechanism of immunity in viral diseases, the future of
specific anti-poliomyelitis prophylaxis seems to belong to vac-
cines prepared with live, modified viruses.
Under the conditions of our country, and on the ba-
sis of recent serologic investigations carried out, it is be-
lieved that vaccination of children against poliomyelitis
should be introduced as soon as possible, as a compulsory me-
thod of prophylaxis.
187. N,CAJAL, EL.OPRESCU, G.FOPESCU. Investigations on neutra-
lizing antipolio velitis II d,ntibodies, St.cerc.
inframicrobiol.,microbiol.,parazitol., 1957, 8,
2, 2o1.
Comparative seroneutralization reactions with the
Lansing poliomyelitis virus and the herpes virus in persons
without poliomyelitis in their antecedents, showed a frequent
parallelism between the titers of serum anti-herpes and anti-
poliomyelitis type II antibodies. Additional investigations
carried out with the sera of dogs plead in favour of the exis-
tence of other viruses with antigenic functions, common to those
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80TOO246AO19200640001-8
112
of the Lansing poliomyelitis virus..
The data obtained showed that some of the posi-
tive results obtained in the seroneutralization test with the
Lansing virus in persons without poliomyelitis in their ante-
cedents should be interpreted with a certain caution. On the
other hand, the fact that this reactivity to poliomyelitis
and the frequency of antipoliomyelitis,antibodies are inver-
sely proportional, fully justifies continuing researches on the
antigenic relations between the Lansing virus and the other vi-
ruses, in order to complete specific antipoliomyelitis prophyr
laxis with a non=specific prophylaxis of the herpes type, a
method advocated by the Rumanian school of inframicrobiology.
188 C=Q11AxINESOU, I.TURCO, AJ.FRIEDMAN, V.CIUREZO, N.DRAGA-
,NESCU. Poliomyelitis myocarditis; Minerva
Medica, 1957, 48, 49, lobo.
Involvement of the heart in poliomyelitis is
an eventuality to be feared, as it aggravates the prognosis.
Circulatory, degenerative and especially inflammatory le-
sioxis stand at the basis of this affection. Eloquent micro-
photographic-documents illustrate this paper. Almost half
of the 52 subjects who died of poliomyelitis, exhibited im-
portant cardiac lesions, which favoured or were even the
cause of death,
1890. GGMARINESCU, I.TURCO, V.CIUREZO., A.FRIEDMAN, N.DRAGANES-
CU, Poliomyelitis myocarditiis (anatomo-clinical
stud .a) La Semaine des H8pitaux, 1957, _2o/3,
3300
Study of 62 lethal cases of poliomyelitis - micro-
scopic examinations were performed in 32 of these cases -
revealed the existence of a typical acute myocarditis in 46.8
per cent of the cases; in 13 cases myocardiac and pericardiac
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
113
lymphopolyblastic infiltrations were prevalent and in another
cases infiltrations with polymorphonuclears were more accen-
tuated..
The highest incidence and the greatest amount of
lesions were as a rule encountered in the first days of the
disease (the 4th and 6th day) in adults and older children,
in cases accompanied by pulmonary complications (interstitial
pneumonia, broncho-pneumonia) or in cases with intense le-
sions of lymphocytic perivascularitis involving almost the
entire nervous system; these lesions were likewise encountered
in several cases that appeared during the last two epidemics
that broke :.out. ,_in r , ;ix1 1953 6hd 1055,
Cardiac insufficiency that accompanied the ner-
vous disturbances was the decisive complication in at least 3
cases.
With a view to the pathogenic interpretation of
these. cardiac lesions, the authors discuss the various factors
that may intervene alone, simultaneously or successively:
tropism of the poliomyelitis virus,, extension of the lesions
of the nervous system, pulmonary complications,- superimposed
infections, biochemical disturbances, the stage of the disease
and age of the patient. Some of the properties of the polio-
myelitis virus and particularly its cardiac tropism-represent
the most important elements in the onset of poliomyelitic
myocarditis,
19o. I.ADERCA, M.IANCONESCU, N.CAJAL. Cultivation of the
polio ym es litis virus on human amniotic cells
Morphologic changes. St.cerc.inframicrobiol.,
microbiol.,parazitol., 1958, 994, 435,
The cytopathogenic effect is identical with regard
to the morphologic aspect, with all three types of poliomye-
litis viruses, The initial lesions appear in the cytoplasm in
the form of vacuoles of various sizes. In a more advanced
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
114
stage the lesion also comprises the nucleus and the nucleole.
These degenerate and the chromatin gathers into large clumps.
In the final stage, the cells are condensed in
the form of round elements with a slightly acidophil cyto-
plasma surrounding a.pyknosed or karyolysed nucleus.
191. ST.DRAGANESCU, A.PPTRESCU, N.DRAGANESCU, O.BOTEZ, S..CAL-
MANOVICI, GHERASIMESCU. Lethal bulbar poliomyeli-
tis paralysis in a pregnant woman, Lesions of the
reticular substance in the mother. Anatomo-patho-
logic examination of the fetal brain, St.cercvfleu
.rol., 1958, 3, 265,
An ana'tomo-clinical study was carried out on an
acute case of poliomyelitis bulbar paralysis ending in death
after 3 days, due to respiratory insufficiency, in a pregnant
woman in the 6th month, The microscopic examination showed
severe bulbar lesions involving the nuclei of the cranial ner-
ves and particularly the formation of the reticular substance,
both in the group of the medium nuclei and that of the lateral
nuclei. Death due to respiratory paralysis showed however that
in this case the functional damage was greater in the group
of lateral nuclei..
The brain of the fetus was likewise examined and
phenomena of stasis were observed, as well as perivascular
oedema-and oedematous rarefaction of the surrounding tissue
and even discrete hematic extravation
192. G,MARINESCU. Renal lesions in the severe .forms .of polio-
. ._.., ,
myelitis. (Poliomvelitic n.ephritis,)Inter-regional
~r~ ~s~o~acq Rte.. o~Cli. C1 ia~li~
symposium on poliomyelitis, Vatra Dornei.,. May 22-23,
1958 - and Second Scientific session of the Insti-
tute for the Study of Poliomyelitis,, the USSR
Academy of Medical Sciences, Moscow, June 19-21,1958.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
1'l5
The macroscopic examination of 7o cases of polio-
myelitis most of them ascending forms, showed modifications
of a prevalent medullar aspect in the kidney in 58 of the ca-
sese
The macroscopic study performed in 23 cases re-
vealed the presence'of important lesions at the level of the
tubules, glomerules and-renal interstitium in 47,8 per cent
of the cases.
The direct action of the poliomyelitis virus on
the renal cell due to its tropism and the effect of adjuvant
factors, affecting the metabolism of the nervous cell (anoxia
caused by nervous, vascular, cardiac and respiratory lesions)
seems to stand at the basis of the etiopathogeny of poliomye-
litic nephritis.
The aggravating role of renal lesions in the evo-
lution of certain forms of poliomyelitis is postulated.
193. GeMA,RINESCU. Hepatic lesions in severe forms of polio=
Myelitis olioat el .tic hepatitis) Q (Physic -patho-
logic and therapeutic deductions.)The fifth Euro-
pean Conference of Poliomyelitis. Madrid, Septem-
ber 1958; The third Conference of Czechoslovakian
virologists, Bratislava ? Smolenice, October 14-17,
1958
The occurence of hepatitis is described in the
course of severe cases (lethal) of poliomyelitis, in a pro-
portion ranging between 7o 8o per cent.
Hepatic damage is represented by: severe degenera-
tive lesions (as a rule fatty degenerescence), inter-, peri?
or even intralobular lymphohistiocyte or lymphoplasmocyte
inflammatory infiltrations, hypertrophy and multiplication
of reticulo-endothelial cells, discrete connective reactions
and important vascular disturbances (stasis and especially
oedema).
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
116
The author postulates that owing to its physio-
pathogenic cop.sequences, hepatitis greatly aggravates the
prognosis of the ascending or respiratory forms of polio-
myelitis.
Besides reanimation and the well-known treatments,
a protective treatment of the liver should likewise be applied.
194.. QeMARINESCU, D,SARATEANU, E.NASTAC, E.OPRESCU,,B.FUHRER.
Hepaticreacti determined by certain
viruses followi inoculation into the
portal vein of the -rabbit. Third Conference of
the Czechoslovakian virologists.., Bratislava -
Smolenice, October 14-17, 1958.
After inoculation of the MM and Coxsackie viruses
into the vena porta of rabbits, the presence of interstitial
hepatic lesions, representing the defence mechanism of the
organism, were observed under anaesthesia and laparatomy (re-
action of the reticulo-endothelial cells and lymphocyte in-
filtrations in the portal space.)
Mention is made of the part played by the Kupffer
cells in the diseases due to the MM and Coxsackie viruses..
195. ACAD.ST.SJNICOLAU. Data and considerations on the para-
immunity relationship between poliomyelitis and
herpes. St,cert.inframicrobiol.,microbiol.,para-
zitol., 1958, 9-, 3, 293.
An attempt was made to determine the influence of
the state of antipoliomyelitis immunity on experimental herpes
and the influence of herpes the evolution of poliomyelitis,
by means of skin herpes inoculations.
The,great number of negative rbsults, starting from
the second. month after the'beginning of poliomyelitis, shows
an initial antiherpes resistance, enhanced by paraimmunity
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
117
brought about by the onset of antipoliomyelitis immunity.
This unquestionably shows that in poliomyelitis,
besides the specific refractory state to the poliomyelitis
virus (immunity), there is likewise a non-specific, hetero-
ogous resistance (paraimmunity) to the herpes virus.
It was also demonstrated that antipoliomyelitis
vaccination with the Salk vaccine induces in the organism
resistance to arhythmic recurrent herpes.
Para-immunity between herpes and poliomyelitis
raises the problem if experimental herpetization might not
be made to play a part in antipoliomyelitis control, parti-
cularly where antipoliomyelitis vaccines are not available.
196. N.CAJAL, I.ADERCA, M.IANCONESCU, E.OPRESCU, A.BIRCA.
Incidence od seroneutralizing antibodies to anti-
,
genic strains I. II, and III of the poliomyelitis
virus in the inhabitants of Bucbaxw~ St.cerc.
inframicrobiol., 1959, lo, 3, 281.
A study was carried out on the incidence of sero-
neutralizing antipoliomyelitis antibodies in the inhabitants
of Bucb. st, that had not been vaccinated or had not suffered
from clinical forms of poliomyelitis.
Seronetttralization was performed in cultures of
human anbryo cells obtained by trypsinization; 137 sera were
ermined: 8o.3% presented antibodies for at least one type
of poliomyelitis virus, and 19.7% were negative. Of the po-
sitive sera, 31,8% were positive for a single immunologic
type of poliomyelitis virus; 35.4% were positive for two
types and 32.7% for three types. The younger age-group pre-
sented two-stages: between 1-3 months persistent maternal anti-
bodies were found in 87% of the cases, between 4-6 months
incidence decreased to 14% and the antibodies were almost ab-
sent (5%) between 7-12 months. Starting from the age of one
year, the incidence of antibodies swiftly increased especially
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
118
those to type I. attaining loo% in the age-group of 1-2 years,
a percentage that is maintained after 2o years. The authors
recommend starting antipoliomyelitis vaccination at the age
of 3 month""
197? EL.LISSIEVICI-OPRESOU, The anatomo=pathologic study,
of po1,el .ti.s in mice. Lesions of the reticulo-
endothelial aystez, St?cerc,,inframicrobiol.,.l959,
o 3, 3t -ia
A study was carried out on the lesions of the re-
ticulowendotheli,al system in mice, inoculated by intracerebral,
route with the poliomyelitis virus II (Lansing). The material
used was brain and spinal cord collected at various moments
after inoculation, the sections obtained being stained by the
Maim, hematodilin-eosin., Giemsa and Nicul ascu-H?tne't methods ?
Analysis of the material showed that the morphologic substrate
of experimental poliomyelitis in mice is represented not only
by the well known neuronal lesions, but,also by endothelial
and neurog1yal alterations. The latter appear at an early
date - already in the pre-paralytic period and are unequally
distributed, the tirus presenting an affinity for certain areas.
, ;r,0 c rit?rio& O g this affinity does not seem to be of a
functional order, since the lesions may be found both in the
motor areas and in the areas affected to the sensitive and ve-
getative functions.
The. type of these changes is hyperplastic: turges-
cence and hypertrophy of the endothelial elements and modifi-
cation of their shape- in some cases, endothelial elements
detached from the capillary wall can be detected in the ps i-
vascular parenchyma,,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
119
138.N.CAJAL,
Pao IANCONE$CU s I. ADEBC*, G.DANIELESCU, A.BIRCA, Study d
the innideftee of antipoliomyelitis antibodies
type I. II and III in nOn-vaccinated persons
from rfra1 localities in the Rumanian People's
Republic. St.eerc.inframicrobiol.,196o, 11,1,21.
This paper, completing previous studies concern-
ing the incidence of antipoliomyelitis types I, II and III in
the population of Bucharest, deals-with a study in human embryo
cell cultures of the proportion of these antibodies in 3 vil-
lages in the country, in which paralytic polio cases were only
exceptionally reported.
Neutralization tests with the sera of 135 indivi-
duals of different sex and ages showed that:
- The degree of occult immunization to poliovirus
is very high also in rural environment (74.82 per cent of sera
contain antibodies for at least one antigenic type of polio-
virus).
- In the first years of life, a predominance of
types II and III exists, especially of type III.
- Antibodies type I, in contrast to.findings in
urban environment, do not increase explosively, but slowly and
progressively, exceeding the level of antibodies for the other
poliovirus types only after the age of 12.
i99 : (;AJ.A"L9ADERCA, M.IANCONESCU, E.OPRESCU, G.DANIELESCU, A.
BIRCA. The incidence of antinolioelitis antibo-
dies in children in the Rumanian People's Republic.
Microbiol.,Parazitol., Epidemiol.196o, 1, 47.
In order to establish the incidence of seroneutra-
lizing antibodies to the 3 poliovirus antigen types in the in-
fantile population of the country, investigations were carried
out in Bucharest and in 3 villages.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
12o
The sera of 219 children of different sex and
ages, not vaccinated. against poliomyelitis and , which did not
present in their antecedents signs of poliomyelitis, were
used for neutralization tests against poliovirus types I, II
and III (loo CID50), done in human embryo cell cultures.
The results showed that of 219 children, 187 pre-
sented antibodies to at least one poliovirus type (85.3 per
cent), which proves an intense occult immunization due to
clinically unapparent diseases. Thus, the incidence of anti-
bodies to at least one poliovirus type, which was very high
in the first 3 months of life (due to maternal antibodies),
gradually fell in the following months, minimum values being
found in the 7-12 months age-group; at 1 year, these values
suddenly increase,. attaining the level of maternal antibodies
and continue to increase with time, so that in the 13-15 years
age-group no negative serum is found,
In terms. of the antigenic type,, childhood is cha-
racterized by predominance of type I in Bucharest,. and type.
II, and particularly III, in rural environment.
2oo.d GeMARINESCU, I.DINU, I.PREDESCU, S.DIACONU, M.STARK,
A1MEROVICI, S.NICOLAU, M.IVOPOLm ' Comparative
electrophoretic modifications in the sera of
vaccinated and non-vaccinated .noljom,Yelitis
patientse The 4th scientific session of the
Institute of Poliomyelitis, Moscow, May i'/-2i,
1960?
Electrophoretic examination of the protein, frac-
tions in 45 sera samples from 3o poliomyelitis patients, 9 of
whom vaccinated against poliomyelitis-with Salk vaccine, showed
in general hypoalbuminemia with increase of gamma2-globulins
in the acute stage of the disease and gamma-globulins in a
late stage,
In a smaller number of cases (lo per cent), total
hypoproteinemia was observed and in a more severe case, bypo-
globulinemia with hyperalbuminemia,, As a rule, beta-globulin
values were higher in the mdld. and favourable cases.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80TOO246AO19200640001-8
:121
The slectrophoregrams of previously vacinated
vourable prognosis:L'
`further argument, apart 'from he epidemiolog3 c"-and clinical
factors, for the application of ;the live attenuated virus
vaccine. In genes.l.,.gaimma-globulin values are higher in the
first weeks of`the'diseasein=vaccinated patients, increasing
ustill.'.fart in convalescence acid staying `level. for a long time,
by-comparisoi~"w th'the non vaccinated patients,`examined"at
the same stage of the disease, In a lethal case, which had
been vaccinated against poliomyelitis; beta-globulin values
were.
s. ~.: _were' high; in " n:t>u-vaccinated patients this points to a fa-
'... ijL:
"2o1. ACAD.ST.S.NICOLAU; N CAJALM.CEPLEANU, I.ADERCA, N.M
concerning the peritoneal,polio~virus neutralization
~rrrW~~on
t, ees_t. Comun.Acad.R.P,R 1960, to 12 1157.
A.pp1 cation:inexperimental poliomyelitis .of the
test: previously used by S.Nicolau-. and. co-workers in=rabies,
Balled the "peritoneal neutr`alization test" showed ; its utility
for assessing the state of immunity induced in the mouse organ-
ism by different''poliovses and vaccines (wild viruses type I,
'~
fiI,
III mo(
poliomyel1tis
The peritoneal test is highly type characteristic,
and ihtensely:positive"after immunization with wild or "atte
nuated" polioviruses, and negative after, attempts at immunizes-
tion' with' inactivated virus ' vaccines, , of the '-Salk type..
It' is concluded that the peritoneal test ?'can re:-
place the classical neutralization test, which is difficult r
to apply-*indiv'idually to small-'laborat'ory animals, and thus
becomes a useful method for immunologic invent gations in
UXLV rV.L.tvwy Vl.l U.L5 LUb-U1-UU- a 01 Moscow*)
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
CONSTANTINESCU; M.IANCO CU. Investigations
nada; lot`1o8-l: and the live Sabin virus-vaccine prepared in t 1,
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
122
2o2. N.CAJAL, M.IANCONESCU, M.CEPLEANU, I.ADERCA, M.GRUTA,
G:.MARINESCU, C.BABA, Y.COPELOVICI, G.POPESCU, G.
DANIELESCU, 0.BURDUCEA, P.ATAANASIU, M.CIOBANESCU,
Y.PEIULESCU. Investigations on seroneutralization
antibodies in persons vaccinated against poliomye-
litis with inactivated and live "modified" viruses.
Report read at the Symposium "lo years from the
founding of SANEPID" Bucharest, April 28, 1961.
In 196o, the authors administered experimentally
to 1,2oo children the vaccine prepared by the Poliomyelitis
Institute of Moscow, with A.Sabin's-strain.
Interesting conclusions were drawn from the re-
sults obtained is a comparative study on the evolution of
neutralizing antibodies in boo of these children and 371 sera
from children vaccinated with the Salk vaccine, on the one
hand, and on the other from children that had not been previous-
ly vaccinated.
In the latter children, without poliomyelitis in
their antecedents, neutralizing antibodies against at least
one antigenic type, were found in a proportion of 85.3%.
Tn the children vaccinated with the Salk vaccine,
the proportion of positive sera was similar (82.5 per cent) and
paradoxically, in some age groups (1-2 years) even lowed. In
children vaccinated (1-7 years) with the live virus vaccine,
the incidence of positive sera was 92 per cent. In the same
age-group, the Salk vaccine gave an incidence of positive sera
of 76.5 per cent.
Of 37 trinegative children, 32 (86.4 per cent) ela-
borated antibodies for the type of virus in the vaccine.
Virus elimination in feces was almost constant in
the trinegative cases and inconstant in the children with posi-
tive sera previous to vaccination.,
The cktldren with serum antibodies induced by.admi-
ni.stration of the Salk vaccine, regularly eliminate the virus
following its multiplication in the intestine, in opposition
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
123
to those immunized by occult, unapparent infections,
The results point to the superiority of the vac-
ciJae prepared with live, modified viruses, and its obligate
application on a wide scale,
2o3. N.CAJAL, M.IANCONESCU, I.ADERCA, E.OPRESCU,. G.DANIE-
LESCU, A.BIRCA. Comparative investigations on
the incidence of antipoljomyelitic antibodies
in vaccinated and non-vaccinated children in the
Rumanian People'sRepublic St.cercvizxframicro-
biol. 196o, 11, 4, 549..
A comparative study in human embryo cell cultures
of the incidence of polio-type neutralizing antibodies in
371 children aged 4 months to 7 years, before or after anti-
poliovaccination with the inactivated vaccine, showed no
factual differences between the two groups.
Moreover, investigations on antipolio antibodies
in 45 children of a closed community, carried out 6 months
after vaccination showed that in spite of the vaccination,
33 (73 per cent) of the children were trinegative and only
12.(27 per cent) presented antibodies for one or two polio-
virus antigen types (13 per cent for types I and II, 6 per
cent for type III),
In view of these results, the authors recommend
substitution of the inactivated Salk vaccine by a vaccine
prepared with live, modified viruses.
2o4. G.MARINESCU, P.ATHANASIU, L.PREDESCU, C.TAINDEL, C.$0-
CIRNFA, Lesions of the central nervous system
in patients with the res iratory form of poliomye-
litis, maintained for a loin time in ies,iratory
apparatus, Report read at the Institute of In-
framicrobiology, November 2o, 1961.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
124
The clinical evo.Lution of two patients suffering
from a severe respiratory form of poliomyelitis, who were
maintained for about 6 weeks in a respiratory apparatus, with-
out retrocession of the secondary paralyses, is described.
Histopathologic examination showed massive dege-
nerative and older medullar vascular lesions, belonging to
the polio infection and more recent abiotrophic processes of
cerebral malac ia,patognomonie- states of "exceeded coma" a-.
gainst a background of older discrete inflammatory lesions.
2o5.
G.MARINESCU. Recent advances in the specific control of polio-
yelitis. Spitalul, 1961, 2,110..
The latest data concerning the sero- and vaccino-
prophylaxis of poliomyelitis are presented. Improvement in
the preparation of attenuates vaccines and especially the
remarkable results obtained with the live attenuated polio-
virus vaccine are emphasized.
2o6. M.VOICULESCU, G.MARINESCU, I.PREDESCU, P.RADULESCU, A.
RADULESCU. Some poliomyelitis aspects in per-
sons immunized with the inactivated antipolio
vaccine. Spitalul.1961, 2, 167.
Clinical study of 38 previously vaccinated polin
cases showed varied clinical forms, without any difference
with the non-vaccinated polio cases; overall. mortality was
lo per cent in the author's observations and 7 per cent for
the entire country.
No marked differences were observed between the
cases inoculated, with the Lepine vaccine (29 cases) and Salk
vaccine (9 cases), and neither between the completely and in-
completely vaccinated individuals. No polio case was reported
among the children who had received the booster dose.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
125
As a rule, the clinical evolution was more favou-
rable in vaccinated children, the period in which retrogression
of the paralysis occured was shorter and no indelible sequelae
developed.
In more than 2/3 of the cases, favouring factors
were observed: injections in the paralyzed member (9 children),
weakening and fatiguing diseases (13 children), other fac-
tors (4 children).
2o7. G.MARINESCU. On the problem of extraneuraxial lesions
in poliomyelitis infections. Stud.cerc.infra-
microbiol., 1961, 12, supl.,223.
A description is given of various lesions, such as
interstitial myocarditis (46.8 per cent), interstitial pneu-
monitis (3o per cent), interstitial hepatitis C7o per cent)
and glomerulo-tubulo-nephritis (47.8 per cent), occuring in
the course of severe forms of poliomyelitis which resulted in
death,
The direct action of the poliovirus within the
pattern of viraemia and of its viscerotropism, as well as
the existence of factors related to the responsiveness of the
body, the type of disease and its course, age, additional
infectious elements and organ correlations, lie at the basis
of the mechanism resulting in the formation of these visceral
lesions.
The presence of such lesions in severe forms of
the disease may aggravate the prognosis "quo ad vitam" of
poliomyelitis and should, therefore, be well-known, detected
as early as possible and treated in time.
2o8. I.FRIEDMAN, S.DUMITRESCU, I.SAMUEL, T.SANDULESCU. Coxsa-
ckie virus strains isolated from cases of Para-
-
lytic,_2oiiomyelitis in Jt3ueh St. cerc . infra-
ea car e i A~r
microbi.ol.,microbiol,,parazitol?,1955,6, 1-2, 51.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
126
In october 1954, ;wo strains of Coxsackie-virus
were isolated, by inoculation to new-born mice of f'e-cal
suspensions taken from 12 patients hospitalized in the cli-
nic of Contagious diseases Bucharest.
The patients from which these strains were iso-
la ted, exhibited clinical signs of paralytic poliomyelitis. The
anatomo-pathologic examination showed that the two strains of
Coxsackie virus belonged to the G.Dalldorf type.
2o9. B.DAN, I.FRIEDMANN. The Coxsackie virus isolated from
patients with paralytic poliomyelitis. Etiologic
relations, St.cerc.inframicrobiol.,microbiol.,
parazitol., 1956, , 1-2, 51.
The connexion between the Coxsackie virus and
various neurologic syndromes, including paralytic, poliomye-
litis type syndromes, is discussed.
A description is given of two clinical cases (iso-
lated peripheral facial paralysis and flabby tetraparesis with
affection of the Vlth pair) in which the Coxsackie virus was
found in the stools. The seroneutralization test yielded
high antiCoxsackie antibody titres.
The apparent epidemiologic and experimental antago-
nism between the Coxsackie virus and the poliomyelitis virus,
as well as the relative scarcity in the Rumanian People's Re-
public of Coxsackie virus infections - a virus whose ubiquity
is well known - represent the arguments lending support to the
Coxsackie etiology of the paralytic syndrome, of poliomyelitis
type, described in this paper.
21o. R.PORTOCALA. Electrophoretic proteinogram of the muscle
of new-born mice. Changes occuring in the course
of infection with the Coxsackie virus'. Riv.dell'
Inst.Sieroter.Italiano, 1958, a,2, 107. Comuni
carile Acad.R.P.R., 1957, 7, 12, 1o85.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
;27
A comparative electrophoretic study of the pro-
tein fractions extracted from the skeletal muscles of new
born mice and the same proteins extracted from new-born mice
infected with the Coxsackie virus was carried out.
This study made it possible to establish the exis-
tence of three protein fractions, different as regards their
migration speed and temporarily called "?a", "b" and "c'-#
In the course of experimental infection with the
Coxsackie virus, an increase was observed in the proportion
of the "b" fraction, parallel to a decrease in the proportion
of the "c" fraction,
211. J.SAMUEL, N.DRAGANESCU? S..DUMITRESCU. -Research-work
on the Coxsackie v lses. Giorr,Malattie Infettive
e Parassit. Ed.Minerva Medica, 1957, ..Ten strains of Coxsackie virus were isolated by
inoculation to new-born mice of feces suspensions from pa-
tients with various affections; paralytic poliomyelitis, a-
septic meningitis, myalgia, herpangina, influenza,, etc.
From the anatomo-pathologic view-point, one of the
strains presented.a longer period of incubation than the others,
causing histologic modifications of the nervous system; the
others did not produce any massive alterations of the muscular
tissue.
The authors try to establish etiologic relations
between the presence of the Coxsackie virus in the stools of
the patients and certain clinical manifestations, such as para-
lytic poliomyelitis, aseptic meningitis and herpangina.
212. S.MATEESCU, N.CAJAL, Y,COPELOVICI, R.CAPRARU. Apparent
clinical infection with e_.Qoxsacki-evirus ru)
in'the adult white mouse irradiated with X r .s
St.cert.inframicrobiol..,microbiol.,parazitoi:,1958,
9,4, 463.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
128
The effect of u adiation with X rays on the
sensitivity of the 3flult white mouse to the pathogenic ac-
tion of the Co: - &; k:ie virus group A is studied. The animals
were irradiated with a Therapix apparatus with a 24/24 cm
radiation field, at a distance of 4o cm., during 3o minutes,
each animal receiving during a single exposure a total dose
of 6oo r. (180 KV, lo mA, o.5 mm Cu filter).
After irradiation, the animals were inoculated by
intracerebral or intramuscular route with a 20o LDS, of Cox-
sackie virus suspension (A2). The same virus dilution was
inoculated at the same time to non-irradiated adult animals.
The results showed that 66% of the irradiated ani-
mals inoculated by cerebral route, contracted the lethal para-
lytic disease. The biologic tests showed that the etiologic
agent of this paralytic affection was the Coxsackie virus. A
study of the virus in several organs and tissues (brain, pan-
creas, muscles, etc.) showed that the virus persists longer
in the irradiated than in the non-irradiated animals. Titra-
tion of the virus likewise showed a more intense multiplica-
tion of the virus in the tissue of rats submitted to irradia-
tion.
The results of the experiment raise the problem
of the possibility of carrying out experiments with the Cox-
sackie virus on irradiated adult mice.
213. I.SAMUEL, N.DRAGANESCU, S.DUMITRESCU. The presence of the
Coxsackie virus in clinaA. effactions with" di.f="
~?~r~r~riirr ~i irrrife "rant ` charactara: " St: care: inf rniagabiOl; ;inicro-
biol.,parazitol:, 1958, 9,2, 193?
Ten Coxsackie virus strains were isolated from the
feces of 186 patients, hospitalized in the Clinic of conta-
gious diseases in Bucharebt #during the November 1954 - June
1956 period, with a diagnostic of poliomyelitis, meningitis,
herpangina, influenza and 4 poliomyelitis contacts.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
-129
Sepa7ration and identification of the infectious
tcsv was- riot possible with two of these strains.
0?? the 8 other strains, 2 were isolated.from 2
children with benign paralytic ;poliomyelitis; . one strain was
isolated from a child that-had been in, contact with- ,a polio-
vqelitis case during the 1957 summer epidemic; 3 strains, were
isolated from 2-children and one adult with a clinical diag-
nosis of aseptic meningitis or meningo-encephalitis and, fi
nally, 2 strains from 2 children with a clinical diagnosis of
herpangina.
From the anatomo-pathologic view-point, only one
of the lo strains isolated produced minimal lesions of the
muscular tissue and discrete modifications in the central
nervous system-. ' ".The -other 9 strains gave rise to massive le-
sions in the muscular tissue, without alterations of the cen-
tral nervous system.
214. N.CAJAL, S.MATEESCU, Y.COPELOVICI, E,PACURARU. Trans-
mission. of the Coxsackie virus A2) to adult
White, !nice treated with-- :- Comunioariie--
Acad.R.P.R. 1959; x,'1,-81:
Wishing to establish if internal. irradiation with
beta rays can influence the sensitivity of the adult mouse
to the pathogenic action of the Coxsackie virus (A2), strain
which is not normally pathogenic to the adult mouse.-the au-
th.ors inoculated by intracere'bral route 20o LD50 of a Cox-
sackie virus suspension (A2) to adult white nice, which had
been previously injected with an isotonic. solution of P32
(Na2HPO4), in a dose calculated for an activity of 50 C?
The experiments showed that intern, irradiation
with the beta rays emitted by radioactive phosphorus (P322)
sensitizes. . the adult white mouse to the pathogenic action of
the Coxsackie virus (A2), Vitus which is usually pathogenic
only for the. new-born animal,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
13o
After intraper_.t deal introduction of a radio-
active stlution of Na2HP04 (P32), inoculation by cerebral
route of the Coxsackie virus (A2) gave rise-in 81.8% of the
animals to:a paralytic lethal disease, with prolonged persis-
tence.of the virus in certain organs: and tissues'(brain, pan-
creas, spleen,.:'-kidneys, muscles) 'as compared to controls.
These results are due, in the opinion of some
authors, to a modification of the resistance of the animal
organism under the action of beta rays and to a more intense
multiplication of the Coxsackie virus in the body of animals
treated with P32.
215. N.CAJAL, S.MATEESCU, X.COPELOVICI. Sensitization. of
adult white mice to the pathogenic action of the
Co "sackie-Viras" fo1loWing- foni2ifag^ irradiation
(RoeStgeh anQ"beta Fays:)"Acts"~tiroiogica"1959,
'The effect of radiations (Roentgen and.beta rays)
on the sensitivity of 14-18 gr adult white.mice to, the patho-
genic action of the Coxsackie virus, type 2 group A, inocu-
lated after irradiation, is studied.
Irradiation was applied externally and internally.
For external irradiation (Roentgen).'a boo r dose was applied,
employing a Therapix apparatus, 18o kv, lo mA, 0.5 mm Cu fil-
ter, at 4o cm. distance, 24 x 24 cm. localizer, during 13
minutes.
Internal irradiation (beta rays) was done with
Na2HP04 by intraperitoneal injection of P32, with an activity
of 5o-/uC.
The conclusion. is reached that external irra-
diation with'Roentgen-rays or internal irradiation with
radioactive phosphorus injected intraperitoneally sensitizes
the adult white mouse to the pathogenic-action of the Cox-
sackie A2 virus, inoculated intracerebrally.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
131
Paralysis and. the lethal disease appeared in 82%
of the mice in the case of irradiation with rays (P32) and
in 66% of the cases with Roentgen irradiation.
The mortality rate in controls ranged between o
and o,8%. The virus was found in the organs and tissues of
the irradiated animals for a much longer period than in non-
irradiated animals.
216. R.PORTOCALA. Electron images of Mofibril_ new-born
mice, in the' course"of infedtioiiwith- the-Coxsa
ckie-virus. Riv.dell'Ifist:Sieroter .Italiano-1959,
34, ' 4, .263.
The changes that appear in the myofibrils of
new-born mice in the course of. infection with the Coxsackie
virus (group A) are studied in the electron microscope.
The protrusion of aatomye n at the level of the Z striae,
with disappearance of the N striae and homogenization of the
Q segment, followed-by hyaline degenerescence in the advanced
phases of the disease are successively described; mitochondria
were the only formations revealed in the myofibrils during
this interval of time. An exaggerated fragility of the myo.:.._.:.
fibrils, abundence of collagenous tissue and, in the pre-coma
phase, the appearance of regeneration myofibrils was likewise
observed.
ECHO
217. M.GRUTA, Y.COPELOVICI, I.POPESCU. Isolation of an ECHO
12 virus from a meningitis patient with-exanthema.
Isolation, for the first time in the country, of
ECHO 12 virus, from a child with moderate meningeal syndrome,
is reported. The virus was isolated in trypsinized human embryo
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
132
cell cultures. The presence o.,: ECHO 12 virus is reported for
the first time in serous meningitis.
218. G.MARINESCU, LIU CIUN PAE. Lesions induced by ECHO virus
(tpe 9) in cortisone treated adult and new=born
white mice. Stud.cerc.inframicrobiol. 196o, 11,
3, 4o9.
Using ECHO 9, carried through numerous passages on
monl.ay kidney cell and human embryo call eultures,a soserate myo-
sitis process was. obtained in the new-born mouse inoculated by
intramuscular route and a moderate hepatitis process in the
new-born and adult mouse, inoculated by intraperitoneal route..
. Association of cortisone, 3 days and 1 day before
inoculation of ECHO virus, resulted in diminution: of the
inflammatory and degenerative processes, due to its antiedema-
tous and antiinflammatory effect.
A comparative study should be done with freshly
isolated strains, in order to establish if their pathogenicity
is modified, and if their cytopathic effect may serve for the
diagnosis of ECHO infections.
219. M.GRUTA, Y.COPELOVICI, V.ARMASU, C.LECCA,-V.VASILESCU.
Investigations of the incidence of hemagglutina-
tion-ihhibition-'antibodiesto-certain ECHO virus
strains"in-some-eommnnittes-in-Bacharest:Stud.cerc.
inframicrobioi: -i96o; "Ui 4; - 79;
A study was carried on anti-ECHO hemagglutinating-
inhibiting antibodies in the sera of 385 healthy individuals
from two industrial and a military unit in Bucharest. Anti-
ECHO 7 antibodies were found in 63.3 per cent of the persons
investigated, anti-ECHO'9 antibodies in 36.7 per cent and
anti-ECHO 19 antibodies in 31 per cent.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
133
A parallelism was seen to exist between the inci-
dence of anti-ECHO 9 and 19 antibodies, however With differ-
ence in the titers.
22o.. M.GRUTA, Y.COPELOVICI,.V.CIOBANESCU, MOGROZA, M.DAMIAN.
Comparative study of complement fixing and h m
agglutinotiot~-iahibitink'b tibbdies to-ECHO ?11
and ECHO 19 viruses in a" childi*h1 b d62M h1tt:
Stud.cerc.inframicrobiol.,1961,'12, suppl.,2o3;
Rev.Sci.Med...1961, 6,.1-2, 53.
In a,closed children's community, hemagglutination-
ir_hibiting and complement` fixing antibodies to ECHO 11 and
ECHO 19 viruses were found to be totally absent in the o-6
months age-group.'.Low-titre antibodies to ECHO 11 antigen
(1/32 - 1/64) and higher titres to'ECHO.19 antigen were de-
monstrable in the-hemagglutination-inhibition test.
The almost similar results of both tests determine
the authors to suggest the hypothesis that within the ECHO
virus group the complement fixing test may be considered as
type-specific.
221.. G?.MARINESCU,,.G.SORODOC, D.SARATE,pNU, S.DUMITRESCU.
A st dv on th`gglutinatingProperties of cer-
tain ECHO":~iiiis strsibs"ystsufi'$4lflafl"af`ytfb@ytes"
of different grbttbs~8hd the ex`vtFociites"sf'sb~ne"
animal .spec ie'Stud,cerc:iriframidt6bidl:"1961,
12, suppl, 2151 Rev.Sci.Mgd. 6, 1-2, 61.
A description is given of the agglutination reac-
tion of type 11, 12, 19 ECHO virus strains with human red
cells belonging to various: groups of the RH-and ANO-systems.
As a rule, no titre' differences were recorded. Obviously the
reaction is dependent on the cell receptors involved in the
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
134
enzymatic process occuring at the level of the red cell and
has no relation with the specific..blood,.group.antigens.
The agglutination reaction of some ECHO virus
strains'wich strongly 'agglutinated human erytrocytes, yiel-
ded weakly positive and inconsistent results and low titres
(1/l0 - 1/20) with the red cells of puppies, rabbits, hamsters
and mice. Two of the 6 ECHO virus strains yielded moderate
agglutinating titres,(1/4o - 1/80) with monkey-'red cells. This
raises. the problem of. the heterogeneity;sand'possible correla-
t~ on . of human orphan, viruses with animal strains.
222. G.MARINESQU, P.ATHANASIU, O.MITROIU. Interstitial
pneumonia and experimental encephalitis-ih-hew-
born white' mice;`3.noculated'with"the-hemagglutina-
tingECHO ll virus. Report-read at-the Ihstitute'
of Inframidtbbiology. October 16, 1961.
Inoculation of the hemagglutinating ECHO 11 strain
into the cervico-dorsal lipo-Muscular mass, induces in new-born
white mice encephalitis and interstitial pneumonia lesions.
From the lung and brain of the mice sacrificed l and 3 weeks
after inoculation, the virus was-isolated by inoculation into
monkey kidney cell cultures.
2239 M.K.VOROSHILOVA, G.MARINESCU, V.I.JEVANDROVA, M.S.BALAIAN.
Investigations on. the hemagglutination of some
ECHO virus strains" isolated ifl"-the' USSR dui-if g
antipoliomyelitis: vaccifiatiozi.: Report read" at"
the Institute of lhfp picrobiology, July 4, 1960.
Hemagglutination with human group 0 erytrocytes
of 16 ECHO virus strains (types 8, 11, 12 and 19) isolated
in-the USSR from the feces of children immunized with the live
attenuated Sabin polio-virus vaccine, was intensely positive
for 6 virus straths (two ECHO 11 strains, three ECHO 12 straits
and one ECHO 19 strain.)
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
135
No-definite parallelism was observed between
the hemagglutina:ting.and infective potential of these strains.
Hemagglut.nation-inhibition with type hyperimmune
sera yielded much higher titers than in the controls; inhi-
bition of cross. hemagglutination gave very low titer:st which
confirms the specificity of the test and the possibility of
using it for. diagnosticI purposes.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
VI
NEUROVIRAL DISEASES
224 - 339
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
136
224. R.PORTOCALA, N.CAJAI,. Histopatho1ogic alterations of
the retina neurons"following"experimental~i~t="
fection- with- the . herpes-virus '- Bul.Stiint. "
Acad.R.P.R., Sec tt St.Med. -1.949, 1, 5, 411.
Two strains of herpes virus, a neurotropic and
a dermotropic strain, were inoculated to rabbits on the sca-
rified cornea or by intracerebral route. The cellular le-
~sions, produced by the herpes virus at the level of the re-
tina, were studied. The nuclei of the. neurons that constitute
the chain of ganglionic cells are the site of alterations cha-.
racteristic of the herpes virus when it is inoculated intra-
cerebrally. The virus propagates by septinearitla route along
the optic nerves, reaching the retina, where it localizes in
the nuclei of the ganglionic cells; here it multiplies and
produces veritable colonies and typical inclusions. The lon-
ger is the incubation period of the experimental disease.,. the
greater is the number of neurons affected.
Inoculated on the scarified cornea, the herpes
virus did not give rise to any histopathologic alteration in
the retina. The route taken by the germs from the inoculated
cornea to the'retina being longer in this case, it explains
why the rabbits infected by the corneal route do not present
herpes alterations of the retina.
In the light of the data of this paper, it is
assumed that "cortical blindness" of experimentally infected
rabbits is due to a great extent to the alterations induced
by the herpes virus in the retina and that certain distur-
bances encountered in human ocular pathology, whose etiology
is not well established, could be explained by localization
of the herpes virus in the retina and induction of certain
lesions in the ganglionic neurons.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
137
225. ACAD.ST.S.NICOLAU, G.BALMUS, N.CAJAL. Transformation of
the herpes recidivans virus into' herpes _ simplei
virus bt'adaptatiOfl tb-the-tabbit"brsin; ------
c:inframic20bi01:; 1950;'i;~
2;-137.
The herpes virus, collected from the vesicles of
2 herpes recidivans patients, was inoculated by swabbing on
the scarified skin of 4 persons. After 3-4 days, herpes with
a relapsing character appeared in 2 of these persons. On the
other hand, the initial virus was also inoculated to a group
of rabbits on the scarified cornea, causing a keratitis fol-
lowed by typical encephalitis. A number of 5 serial passages
were performed on the brain of a rabbit, resulting in a short-
er period of incubation, as well as increased aggressivity of
the virus. Brain fragments from the last passage were inocu-
lated to 5 persons by swabbing of the scarified skin. Inocu-
lation was positive in 3 of the 5 cases and no relapse of the
herpes eruption was observed in these patients, which were
followed up .during 8 months. The induced herpes presentee:
the symptomatology of herpes simplex. Therefore, by adaptation
to the central nervous system of the rabbit, the herpes recidi-
vans strain behaves like.an ordinary herpes simplex strain.
226 N.CAJAL, R.DINU, P.STROESCU. The herpes virus and
catarAct in rabbi.tsw St:cerc:inframicrobio1.,
mietoblo1 pi Ztt01.; 1951, 2, 1, 163.
The authors describe a case of cataract in a
normal rabbit'which had stayed several days in the same
hutch with a rabbit inoculated by corneal route with the
herpes virus, closely resembling-the cases reported by
Nicolau and Koprowska in 1931. About a month after having
being put together with the rabbit inoculated with the herpes
virus, the non-inoculated animal presented a mature cataract
of the right crystallin and an incipient, posterior polar
cataract in the left crystallin.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
138
In order to prove that the rabbit had contracted
a subclinical herpes following contagion in the hutch and had
then become immunized, it was inoculated with the herpes virus
on the left cornea. Twelve days after inoculation, when the
control animals died of herpes kerato-encephalitis, the rabbit
with cataract, which had survived, was sacrified and the brain
examined histopathologically. On the sections, stained by the
Nissl, Spielmeyer and Mann 6-aLod,as he picture of an old herpes
meningoencephalitis was found in the left hemisphere together
with an area of softeninr7 in the occipito-calcarine cortex
and in the axis of Ammon's horn; in the right hemisphere dis-
appearance of the pyramidal cells in certain spots and of
some cells in Ammon's horn could be observed: at the level of
the entorhinal one of the pyriform lobe, herpetic inclusions
were noted.
Both the. mature and the incipient cataract may
be the result of tropl disturbances caused by the lesions
described, a fact which might justify an eventual cortical
representation of the trophic disturbances of the crystallin?
227? ACAD.A..KREINDLER, N.CAJAL, I.OLTEANU? The action of
tellurium in intracerebral iniectl.Of iii"' rrXp~-
rimei~tal " he5rbe~ ~ etace~ihalY.'~is ~ of "' the ~ raT~b~.t ?"
Bu.1?Stiii ~oAcad""R?PoR~ Sep :St~Med:;~I951;'2,
1, 329.
In view of establishing the way in which experi-
mental herpes encephalitis in the rabbit is in-joluenced.by
the parallel evolution of a toxic encephalitis of the tellu-
r;Wa.encephalitic type, a study was carried out on the effect
of an intracerebral injection of black metallic tellurium,
at the height of a herpes neuraxon infection.
The experiments showed that injections of a 1%
black metallic tellurium suspension in small amounts (o.lo ml)
into the brain of rabbits at the height of herpes neuraxon
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
139
infection prolongs the evolution of the disease, sometimes even
leading to recovery. The attempts made at tra=T_'Iing this vi-
ral disease by intracerebral inoculatior,starting from the
brain of these rabbits, yielded negative results, demonstrat-
ing the existence of an autosterilization process.
Intracerebral injection of a larger quantity of
the tellurium. suspension (0.25 ml.) causes death of the animals
in the following 24 to 36 hours. Intracerebral passages of
brain emulsions from these rabbits constantly yielded positive
results.
From the histopathologic view-point, a large num-
ber of characteristic inclusions were found in the first group
..1. animals.
228. R.PORTOCALA, N.CAJAL, E.LISSIEVICI. $yperglycemia followr_.'
ing experimental herpes encephaliti8-1f the' .xabbit.
Comu.nic9rile"'Aoad-.R;P;R:-1951;-1;-507: ------------
Experimental herpes encephalitis in the rabbit,
subsequent to sub-dural inoculation, is followed by a sig-
nificant increase of glycemia. This increase begins on the
second day after inoculation, attaining maximum figures on the
in which the animal dies. Saline solution and normal brain
suspension do not modify the glycemia curve. Glycosuria is
absent in the animals with herpes..
229. N.CAJAL, E.LISSIEVICI. The evolution of glycemia in ex-
perimental herpes infection _ in- tie-Iabbitm - '- -' ? .
St o cerc ~ i::~"~ amicrobiol m .uiicrobiol: ~, parazitol. ,
1952, 3, 3-?4, 161.
The glycemia curve in a group of 15 rabbits ino-
culated on the cornea with the herpes virus is studied. In 9
of the animals an increase of this curve was observed, running
parallel to the appearance of the characteristic keratitis and
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
encephalitis. The glycem a ,:avve continued to rise until
death of the animals occured.
Similar results were obtained in another group
of 9 rabbits, inoculated by intracerebral.route with the
herpes virus, the symptoms of herpes encephalitis developing
parallel to an increase of the glycemia curve.
A comparison is made between the hyperglycemia
obtained under experimental conditions and human diabetes and
the viral etiology of diabetes, which could be caused either
by the herpes virus or by another virus capable of modifying
sugar metabolism, is assumed.
23o. N, CAJAL, R.DINU, S?MATEESCU, G.POPESCU. Evolution of
experimental herpes neuroinfection in rabbits .
with` a'-xnodifi ed ` reactiVit ?of
vous system,: '. ComubicariIa"'Aead;ReP R: "1953;
i., 9=1.0, ~341.
The comparative, study of the evolution of expe-
rimental herpes in rabbits, with a modified reactivity of
the central nervous system, showed that the incubation period
is much shorter in the animals submitted to repeated electro-
shocks and slightly shorter in the animals in which the reac-
tivity of the central nervous system was inhibited by chloral
hydrate.
The results obtained showed the importance of
the cerebral cortex in the defense of the organism against
inframicrobial agents.
231. ACAD.ST.S.NICOLAU, GvIVIARINESCU. On the possibility of
paraspecific prophylaxis by means`tf"exberiffiaetal
herpatizatio~~al~.o~i,~a1i'Cis: "'Re~iart"1~ead."at~""
the Fourth" anti=polio!i7elitIs"'International Sym-
posium, Bologna, September 1956.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
141
Previous researches showed that the reaction of
the organism, spontaneously infected with the poliomyelitis
virus, against experimental herpes inoculation, definitely
indicated a progressive attenuation of the usual sensitivity
to the inoculated virus (duration and aspect of the eruption).
This attenuation which begins in the second month after the
onset of poliomyelitis, is later transformed into a strong
resistance. Based on the assumption of the possibility of
developing a pa.?-~ _i:iwie refractory state against poliomyelitis,
following herpes immunization, herpetization of persons liable
to contamination is suggested. This paraspecific prophylaxis
of Heine-Medin's:. ;P bd performed at the same time
as the specific vaccination.
232. N.CAJAL, C.BABA. Action of vitamin C in experimental
herpes neuro=infection;"St:cerc:infrainicrobiol.,
microbio1;,-paraZitei;, 1957, 8, 1, 23.
Vitamin C administered by intramuscular route to
rabbits inoculated with the herpes virus on the cornea, acts
differently according to the law of large and small Danielopolu
doses: in small doses it prolongs the life-span of experimen-
tally inoculated animals, whereas in large doses it curtails
the duration of the disease as compared to the controls.
The mechanism of action of these differences
seems to reside in the stimulation of acetyicholin and gamma-
globulin production.
233. S.NICOLAU, G.BALMUS, N.CAJAL. Experimental transformation
of the herpes recidivans virus into herpes simplex
virus.. Giornale di Malattie Infettive'e Parassit.
1957, 9.
With a view to transforming the herpes recidivans
virus into a herpes simplex virus, by adaptation of the reci-
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
142
divans virus to the rabbit brain, experiments were carried
out with 2 virus strains from 2 patients with arythmic herpes
recidivans. The virulent vesicular fluid, collected from one
of these patients, was inoculated by scarification on the
skin of 4 persons, 2 of which presented positive results
with attacks of herpes recidivans. The same virulent fluid
was inoculated at the same time on the cornea of rabbits.
The brain of one of these rabbits, which died
of herpes encephalitis, formed the starting point of 6 serial
cerebral passages on rabbits.
The brain of the 6th passage was used for dermal
inoculation to 4 persons, 2 of which presented positive re-
sults: eruption of herpes simplex.
Another similar experiment with vesicular fluid
from another case of herpes recidivans yielded identical re-
sults, demonstrating that, by repeated passages on rabbit
brain, the herpes recidivans virus is transformed into a her-
pes simplex virus,
234. R,PORTOCALA, N,CAJAL, E.LISSIEVICI. Hyperglycemia in
experimental herpes, The National"Congress-of
Med.Sciences Bucharest, SecV.Neurol.,endocri-
nol . , 1957, 620
The influence of experimental herpes virus in-
fection on the glycemia curve is studied in rabbits, after
their inoculation by cerebral and corneal route, From the
data obtained, it results that the rabbits inoculated with
herpes virus exhibit an increase of glycemia, which yields
maximum values in the pre-lethal period.
These experiments show therefore that introduc-
tion of the herpes virus into the organism of the rabbit by
central or peripheral inoculation, gives rise to a locali-
zation of the germs also in the glycoregulator centers, where
they stimulate or even damage the' neuron with subsequent
appearance of hyperglycemia. These lesions are irreversible
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
143
in the lethal cases and reversible in the non-lethal auto-
sterilized cases.
An analogy is drawn between the aspects obtained
in experimental herpes infection in rabbits and those of cli-
nical human diabetes; the possible herpes origin of this di-
sease-is discussed.
235. R.PORTOCALA,.V.VELICICOV, Y.COPELOVICI. Cyto-chemical
investigations in experimental herpes'( eso
ribonucleic acid: `St: cef'c: iSfratfiicrobiol: ffiicro-
biol.,parazitol., 1957, 8, 2, 149.
The study starts from the premise that multi-
plication of the herpes virus requires part of the nucleo-
protein and determines stimulation of desoxyribonucleic acid
synthesis. Experiments were carried out on rabbits inoculated
by intracerebral route with the herpes virus. The brain sec-
tions obtained were stained by the Mann and Feulgen method.
During the first stages of the infection, infini-
tessimal amounts of desoxyribonucleic acid are revealed within
the nuclear virus colonies, As the cytologic changes become
more accentuated, nucleic acid accumulates in the virus colony
to the detriment of the remaining ehromatin. The amount of
desoxyribonucleic acid increases within the inclusions that
appear in the midst of the virus colony. This may be due, on
the one hand, to impairment of normal cellular metabolism, that
tends towards a stimulation of nucleic acid synthesis and, on
the other hand, to uptake of residual chromatin substances du-
ring inclusiogenesis. The results obtained make it possible
to establish an analogy between the mechanism of stimulation
of desoxyribonucleic acid synthesis in the course of the phe-
nomenon of bacteriophagia and the phenomenon determined by pe--
netration and multiplication of the herpes virus in the neurons
of the experimentally infected rabbit.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
236, R.PORTOCALA, V.BOERU, R.CAPRARU. Investigations on
hyperglycemia in experimental"herpes:(Ladaded
by er- and by oglyce~iaa St?cercaiafraffiicrs=
bic14smi6rbbio1m;JaAa2it6l., 1957, 8,3, 341.0
A study was carried out on the evolution of
induced hyper- and hypoglycemia in the course of experimen-
tal hyperglycemia in rabbits, caused by intracerebral ino-
culation of the herpes virus. Induced hyperglycemia was
obtained by oral administration of glucose, 'hypogglycemia
by subcutaneous injection of insulin. Evolution of glycemia
was studied with the aid of the Hagedorn-Jensen microreac-
tion. Under these conditions, the curves obtained in the
animals which showed an accentuated hyperglycemia following
herpes infection differed from the curves obtained in the
control animals. The reactivity of the experimentally in-
fected animals differs to a greater extent from that of the
control animals, the more accentuated is the herpes glycemia.
The aspect of the curves pleads in favour of an approach
between the response of the herpes-inoculated rabbit and
that of persons with aoglycemia, submitted to the tests
of induced hyper- and hypoglycemia.
237. I.SAMUEL. Electrophoretic modifications in the sera of
rabbits experimentally infected with the herpes
virus. Comunicarile Acad-.R.P.R. Sect*iSt..Med;-
1958s 8, 11, 12210
The results obtained in electrophoresis of the
sera of 14 rabbits experimentally inoculated with the herpes
virus, the Bruxelles strain (5 on the scarified cornea and
9 by cerebral route) are reported.
The electrophoretic determinations were done
with an IK?3o apparatus. The curves were interpreted accord-
ing to the method of P.Cazal and G.Carli, obtaining gradients
of the Tisseli .q type.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
145
In the animals inoculated on the scarified
cornea, a decrease is observed in the albumin-globulin
ratio, reflected by a marked increase of betaglobulins
and a constant decrease of albumin, together with slight
modifications in the proportion of alpha and gamma globu-
lins, which as a rule remain within initial limits.
In the animals inoculated by cerebral route,
similar modifications are observed, but of lower intensity.
In this group, the gamma globulins have at the beginning
a marked tendency to decrease and then begin to increase
slightly.
238. I.ADERCA, M.IANCONESCU. Researches on the herpes virus
lished in
in human amniotic ahd emabryo tissue"cfi~tures:
Report-read-at-the-Sed6ad-Cotgro8a-af"Hiingarian
Microbiologists, Budapest, Sept.22-26, 1959-
A brief summary of the data obtained and pub-
our country in previous works on the herpes virus
in cell cultures,.
239. M.CEPLEANU, N.CAJAL. Research on the transmission of
herpes to"the goiden-hamster: (Crieetuss auratus.)
St core: ififrafnicrobi1 a,' 1959, to 3; 349
In two experiments carried out on 51 hamsters
and 8 rabbits, with 2 strains of herpes virus (P 5433 and
Bruxelles), the golden hamster was demonstrated to be an
animal species sensitive to experimental infection with the
herpes virus.
The cerebral and intranasal routes are very
sensitive, whereas cutaneous, subcutaneous, intramuscular
and intraperitoneal inoculation are ineffective.
Experimental herpes infection of the hamster is
reflected morpho-pathologically by a typical meningo-ence-
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
146
phalitin, constantly accompanied in nasal inoculations by
bronchompneumonia of the interstitial type.
24o. G.DANESCU-POPESCU, N,CAJAL. Evolution of experimental
herpes in cortisone treated rabbits:"St.cerc.
inframicrobiol;, 1959, io;1, 71; -
Experimentation with strain 5433 of the herpes
virus on cortisone treated rabbits at various moments, showed
that : experimental herpes infection in this animal species
is greatly aggravated under the action of cortisone acetate.
The influence of the suprarenal hormone is more-
strongly revealed when the animal has been infected by cor-
neal route.
The microscopic examination showed in the cor-
tisone treated animals, in comparison with the controls, di-
minution of the infiltrative reaction, an accentuation of
the congestive reaction and a marked development of virus
colonies, with decrease in the number of characteristic in-
clusions.
241. MOIANCONESCU, I,ADERCA. The herpes virus in cell cul-
tures, III.- Cellular" lesions" firod0.ced.'br the
herpes ~rirus ' 0f~"hCii~afi' embPyo" afifl" aiotic"cell
cultures. " " St. care: iflframicrobiol o ; ' 1959; '10
;
4, 487.
The cytopathogenic effect produced by the her-
pes virus in human embryo and amniotic cell cultures, pre-
sents aspects similar to those of the lesions produced by
this virus in the organism,
The aspect of the cytopathogenic effect is charac-
teristic enough to permit a swift and presumptive diagnosis
of herpes virus, to be confirmed by seroneutralization.
Dissemination of the herpes virus takes place
both directly from cell to cell and by spread through the
culture medium.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
147
242. R.PORTOCALA, V.VELICICOV, Y.COPELOVIC.I. On the
lution of intranuclear desoribonucLeic a=;,',...
in `the"coiirse`of experimental herpes-of the
brain ste~i Rev': Sci Med: 1959; -4.9 -100;----
Experimentation was started from the premise_
that multiplication of the herpes virus may be assumed to
require part of the nucleoprotein in order to determine sti-
mulation desoxyribonucleic acid synthesis. Investigations
were carried out in rabbits inoculated by intracerebral
route with herpes virus. The encephalon sections obtained
were stained.by.the Mann and Feulgen'method.
In the first stages of infection, infinitessima]_
amounts of desoxyribonucle'ic acid were found in the nuclear
virus colonies..-As=.the cytologic alterations became more
accentuated, nucleic acid accumulates in the viral colonies
to the detriment of chromatin remains. Desoxyribonucleic
acid amounts increase within the inclusions that 'appear in
the virus colonies. This may be due, on the one hand;, to
alteration of normal cellular metabolism, which tends to
wards stimulation of nucleic acid synthesis, and on the other,
to a taking up of chromatin residues in the course of inclusion
genesis.
Herpes.,
243. I.ADERCA, M.3ANCONESCU. Investigations on herpes virus
in huzhan amniotic ahd' embryo - celi. cultures, " -
Microbibl ar~2itblo; e.ide ibi?~~96o -2 155a
The susceptibility of human fibroblast cultures,
obtained by'trypsinization of 1 to 3 months old human embryos,
was studied in view of isolating the herpes virus directly
from the patient.. Six strains were isolated from 6 cases
presenting typical herpes eruptions and 7 strains from patho-
logic products obtained from cases of ulcerous stomatitis,
non-diphtheric croup, measles and encephalomyelitis. The
advantages of this method as compared to other methods for
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
148
the isolation of herpes virus are shown.
Morphologic study of the herpes virus infected
cells showed the presence of characteristic lesions, similar
to those in the animal or human organism. These lesions and
their evolution, described in detail, explain the spread of
the virus in the organism and cell cultures.
244. N.CAJAL, HUNG TAO, V.IONESCU, G.MARINESCU, S.MATEESCU.
Electrocardiographic alterations in rabbits
with experimental herpes. Stud.cerc.inframicro-
biol., 196o, 119 21 219:
Electrocardiographic examination of rabbits ex-
perimentally infected with herpes virus showed morpho-func-
tional alterations at the level of the heart.
The low intensity of the myocardial anatomo-
pathologic lesions and the impossibility of isolating the
herpes virus at this level, pleads for a central nervous
origin of the cardiac alterations occuring in the course of
experimental infection.
In view of these results it is assumed that the
herpes virus might play a part in the etiopathogeny of cer-
tain cardiac functional disturbances encountered in the hu-
man clinic.
245? I.ADERCA, M.IANCONESCU, A.BIRCA. The herpes virus in
cell cultures. I.-Isolation-of-herpes-giros-in
human? embryo" cell" cu.Itures: "St~iQ;cerc: i~if~ea="
microbiol?,"1960,"119 -'-2243.
The sensitivity of human embryo cell cultures
obtained by trypsinization of whole embryos is studied in
view of isolating the herpes virus directly from patients.
From the material collected from 13 cases of typical herpes
eruption, 13 virus strains were isolated and identified by
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
149
seroneutralization and the production of keratitis on sca-
rified cornea of a rabbit. Another 7 herpes virus strains
were isolated from products obtained from ulcerous stomati-
tis, non-diphtheric croup, measles and encephalcmyelitis
cases. The advantages of this method over other methods
for the isolation of herpes virus are discussed.
246. M.IANCONESCU. Herpes virus in cell cultures. II.- The
incidence- of _ antiherpes- antibodies; studied.? -
by the"serofie~ittaiiatio8~teactio is tiSaue
____ res:" Sti.d:core:infraffiier8b1oi '196o;"11,
2, 249.
The incidence of antiherpes antibodies is inves-
tigated by means of the seroneutralization test in cell cul-
tures. It is shown that the antibodies appear early already
in the second year of life and attain a proportion that ranges
from 6o to 9o per cent. The value of serum antibodies in
antiherpes immunity is discussed.
247. R.PORTOCALA, N.CAJAL. The presence of Negri bodies
in retina neurons - i.fl' natural "rabies dine - to -
street virus: Bui:Stiiiit;Acad:R:P;R;Sect:St.
Med., 1949, 10,109 891.
The rabies street virus in its centrifugal dis-
semination, gives rise to Negri bodies in the ganglionic
neurons of the retina. These formations could not be found
in the corneae examined, due probably to the small amount
of germs that reach, by septineuritic route, a :odium so
little favourable to the development of the rabies virus.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
150
248. N.CONSTANTINESCU. New data on the prophylaxis of rabies.
Rev.St,Med.9'.i950;"Ii1__8l9;' ____.__...._... ~_._._.__.
General report on the problem of specific and
non-specific prophylaxis of rabies in man and animals..
249.
N.CONSTANTINESCU, A.TOMA, C.DRAGOMIR. The process of
Nektigenesis and alterations of. the"netzt6h_ _.
in"rabies; "AL;Acad;R-OPaR;-SeeV:St;MOd.;;1950,
2g-15'345.
The morphopathologic study of cellular altera-
tions and the inclusiogenetic process showed that no rela-
tions exist between the frequency of the Babe*-Negri bodies
and the intensity of these degenerative processes in the
neurons. The latter process involves not only the nucleus,
but also the cytoplasm of the.neuron and its frequency seems
to be conditioned by the duration of the paralytic phase of
rabies. The specific nature of the cellular alterations
were confirmed in the spermophil., an animal species whose
neurons react by a massive inclusiogenetic process, even
in rabies with the fixed virus.
250. ACAD.ST.S.NICOLAU, MIRCEA V.BABES. "The Babe*-Negri
bodies" and not "the Negri bodies":"Rumanian
science-is first-in the discOvery'of rabies
Inclusions' Bul.Stiint.Acad;R.PoR:Sect:St:Med.,
195o,2 g 9, io75-
As far back as 1893, in the first edition of
his "Textbook on the therapy of infectious diseases", pub-
lished by Professor Penzolt in the Fisher Printing-House in
Jena, Victor Babe* describes rabiesinclusions in the cyto-
plas the nervous cells in the form of "small vacuoles,
coat, _.r_.g a hyalin corpuscle", to years before Negri.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
151
It is thus demonstrated that Babes was the first
author to give an accurate interpretation of the rabies in-
clusions.
It is therefore requested that these formations
should in future be named the "Babel-Negri bodies" and not
"Negri bodies", a designation which does not correspond to
the scientific truth.
251. ACAD?ST.S.NICOLAU, N. CONSTANTINESCU, C.DRAGOMIR. ELPUIU
Ax.,t ;i rabies vaccination of dogs with the fixed vi?ixs
attenuated.:" by "~ tbylic alcohol' and emulsioned
in mineral oils`Afi:Acad:R:P:R:"Sec .St:Med.
1950, 2;_18;"461.
Although the oleate vaccine prepared with the
fixed rabies virus attenuated by 5o per cent alcohol is patho-
genic when inoculated by cerebral route, by peritoneal or mus-
cular route it has no effect. The immunity developed is per-
manent; all the vaccinated rabbits and dogs were immune to the
test=infection with the street virus inoculated-byomuseuler
route (all the control rabbits and 6 of the 7 control dogs
inected by this route contracted rabies), but 2 of the 5 dogs
and 3 of the 6 rats resisted to the cerebral inoculation, which
killed all the control animals.
252. A'CAD _oL,'.ST.S.NICOLAU, N.CONSTANTINESCU,tDRAGOMIR ;E.PUIU'.
Comparative study of septineuritis and "closed
rabies" in vaccinated' and' nbn=Veceinated" nabid
animals and'men -`Ab:Aoed;R.P;R;Sec4 St:Med:195o,
2, 20,..617:.._..
The investigations carried out on 7 human cases
of rabies and 54 animals, experimentally infected with the
street virus, showed that rabies septineuritismanifested by
the presence of the virus in the peripheral nerves, is negative
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
1.52.
in the vaccinated persons. Therefore, the street virus.is
present in the encephalon, but does not spread centrifugally
through the intermediacy of the nerves to contaminate the sa-
livary glands.
In vaccinated cases, clinically apparent rabies
becomes a non-transmissible infection, the saliva of the man
or animal is no more virulent, constituting therefore an ac-
tual "closed" rabies.
Negative septineuritis and consecutive closed
rabies was revealed in 86% of the vaccinated animals and men,
which however died of rabies, as compared to 2o% reported in
n6n-vaccinated cases of rabies.
The phenomenon is explained by tissular immunity
of the peripheral nervous system, acquired by vaccination and
prevention of centrifugal spread of the street virus from
neuraxon to the peripheral nervous system resulting in nega-
tive septineuritis and closed rabies, non-transmissible by
the salivao
'P- 51 3, ' ACAD ,.ST.S.NICOtAU, N. CONSTANTINESCU, C.DRAGOMIR.
Rabies with -a long incubation in vaccinated
per_cns __StocercoinfranigerOb1OY:;_i9ga;~.
1-29"17.
Two cases of human rabies are described, cor-
rectly treated with anti-rabies vaccine in which however
the disease developed after a long period of incubation, of
respectively 158 and 738 days. Experimental data showed that
failure: of the antirabies treatment was not due either to
the existence of an antigenic variant - different from the
street virus - or to a "reinforced" virus strain.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
153
254. :.: ACADy.ST.S.NICOLAU, N.CONSTANTINESCU, CONSTANTA
-DRAGOMIR. Study of the h;.stologic lesions in
the brain of experimental animals in )inapparent
rabies infections St.cerc.inframicrobiole,195o,
Experimental investigations and histo-patholo-
gic examinations, made evident the existence of an "'Inappa-
rent rabies infection". Although rabies symptoms are absent
and the animals infected with sub-rabigenic doses survived,
this inapparent infection exists even after 3-4 months, and
oxyphil degenerescence lesions of the encephalic neurons
can be observed, infinitely more intense than in lethal ra-
bies in which the inflammatory process is predominant, due
to the short evolution of the disease.
The fixed or street rabies virus is in latent
conflict with the neuron, determining the oxyphil degeneres-
cence reaction, compatible ith the apparent state of health.
The cellular alterations, observed in ~tnapparent
rabies infection, explains the state of cerebral tissular im-
munity discovered by Nicolau and other authors in rabies.
255. P.STROESCU. The presence of rabies inclusions in the
carotid" lomfls' ifs-fah" aDd- afiima18 - that" died-
of" rabies: "St: a +c: iflP~affi1cPabIOl:;i~icgOblol.,
parazitei., 1951, 2, 1-4, 117.
The histopathologic investigations carried out
on products obtained from human or animal cases which died
following rabies with the street virus, showed the follow-
ing in the intercarotid glomus :
Rabies inclusions are revealed in the cyto-
plasm of the nervous ganglionic cells, in the thickness
of the nervous periglandular plexus or in the cells that
constitute the sympathetic microganglia.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
154
These formations are likewise to be found in
the rare ganglionic cells encountered within the inter-carotid
gland and interglomerular plexus.
In the glandular cells proper, the rabies virus
could be detected in the form of inclusions in a single hu-
man case.
In the rabies infection, the inter-carotid gland
cells are in a state of hyperfunction, either due to the
presence of the virus, or to incitation of the ingected
afferent nerves.
256. N.CAJAL, S.MATEESCU. Intranuclear Babes-Negri bodies,
S t. care . inframicrObiol: ; ~iie obioi ; paDa itoi: ,
1952, 3, 3-4, 200.
Experimentation with a street rabies virus
strain with long incubation (21 - 23 days by intracerebral
route and 47 - 53 days by muscular route), showed in 3 rabbits,
besides numerous cytoplasmic inclusions, the presence of few
inclusions situated in the nucleus of certain neurons and of
certain glial cells. There were one, two or three inclusions
in one nucleus, measuring approximately 1-3 /h in-size, with
a more or less homogenous aspect and surrounded by 'a charac-
teristic halo. The nuclear inclusions, stained by the Mann
method, appeared - similar to the cytoplasmic inclusions -
in various shades from blue to red.
On the basis of these observations, it is con":..
eluded that rabies inclusions can no more be classed in the
"cyto-oykon" group, but in the "cyto-kario-oykon" group,
cytoplasmic inclusions being prevalent.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
155
257. ACAD.ST.S.NICOLAU, N.M.CONSTANTINESCU, A.TOMA, I.ADERCA,
E.DUCA, M.DUCA. Experimental rabies infection
with the "neurptropic""fixefl?virts. St:ce~c:
inframicrobiol:;~icrobiol:;parazitol.,1952, 3,
348 k7.
Rabigenous activity following peripheral ino-
culation of a fixed rabies virus strain, modified by adapta-
tion to the brain of the ground squirrel (Citellus citellus)
and of the rat is studied. This strain is extremely virulent
and becomes exceptionally aggressive, causing rabies paralysis
in 2o per cent of the rabbits and 42 per cent of the guinea
pigs inoculated by subcutaneous route; in loo per cent of
the rats, 75 per cent of the rabbits and 37 per dent of the
dogs inoculated by intraperitoneal route; in loo per cent
of the rats, 5o-6o per cent of the rabbits inoculated by intra-
muscular route and finally 7o per cent of the guinea pigs and
57 per cent of the rats inoculated by intradermal route.
258. P.STROESCU. Researches on.the "Babe*-Koch" formations
in street-rabies:''St:cerc.inframicrobiol.,mi-
crobiOl.,parazitol., 1952, 3, 1-2, 89.
Experimental and histologic investigations have
shown that the granular formations described by Babes in the
nervous cells of animals that died of street rabies, repre-
sent a histologic reality. They appear under 3 aspects :
pulverulent formations, close to the size of the virus, dis-
covered by Babes and later by Remlinger by means of the ultra-
filtration method; slightly acidophil corpuscular formations
containing 1-5 small granulations, more resistant to argentic
decoloration than the corpuscular mass and formations with
arborescent aspects, which are extremely argentophil. The
granular and corpuscular formations cannot be identified with
the live virus itself, but represent evolutive stages in the
formation of the Babep-Negri bodies. These formations are
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
l56
numerous in the ,;ells c the animals infected with street
virus -r. or a~, . r another species; their frequency
of adaptation on the same species.
` he se a rn a .ions represent the morphologic
expression =->. th3 degree of cellular resistance to the ac-
tion of the rabies virus.
259- N.CAJAL, S.MATEESCU, EL.LISSIEZICI, M.CEPLEANU. The
evolution of rabies due to the street rabies
hires, in-rabbits- 8ubnitted' tai-the - action-.of
certain isolated"'or associated stimulents."
(heat - milk). St.cerceinframicrobiol:,micro-
biol-Dparazitol., 1953, 4, 1-2, 79.
Investigations were carried out in 26 rabbits
on the reaction of the organism to the street rabies virus
after an injection with milk or after thermal stimulation
of the skin, as well as after association of the two sti-
mulents
The results of the experiments showed changes
in the period of incubation. As compared to the controls
who died after an average period of 14 days, the group in
which stimulents were applied separately died after a period
of 21-23 days and in the group in which the two stimulents
were associated (thermal stimulation + milk injection),, this
period was of 47 dayse
The authors conclude that when the two stimu-
lents are applied separately, the response of the cortex is
manifested by an increase in the defence reaction, which
does not exceed however a given threshold.
When the two stimulents are applied simultaneous-
ly, according to the technique of elaboration of conditioned
reflexes, the defence reflex is much stronger. This is due
on the one hand to summation of the stimulations' and defence
responses of the cortex and, on the other hand, to the mecha-
nism of negative induction of the previously fixed dynamic
stereotype-
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
p {~ n An ern
157
-'Co. R.DINU, CGIACOB, E.NASTAC. Resistance to rabies in rats
with experimental reflex"epilepsis-'Bu1;StiinV
Acad;R;P;R; Sect, ;St;Med;; "-1953; ..2; 1, 97.
The street rabies virus was inoculated to a group
of 33 rats by peripheral route. Nine of these rats had ref-
lex epilepsis fits induced by auditory stimulations, 7 pre-
sented only motor excitation, 7 were submitted to electro-
shock and the other to served as controls. None of the rats
with reflex epileptic fits had the disease 32 days after the
inoculation. Of the other 24 rats 66% showed. rabies paraly-
sis between the 14-26th days.
The authors conclude that reflex epilepsis increa-
ses the natural non-specific resistance of rats to the rabies
virus.
261. ACAD.ST.S.NICOLAU, I.ADERCA, G..DANIELESCU, P.STROESCU.
Study of the evolution of rabies with the street
virus. In rats" submitted-to_ a" state"'of conditioned
sloe ; BulaStiint;Aea:d-ORaP;R; Sect/vSt;lied., 1953,..
9.
In order to remove the toxic substrate that e-
xists in narcosis-induced sleep, a reflex conditioned sleep
was induced in a series of rats, by means of chloral hydrate
and strong light. After establishing the conditioned reflex,
the rats were inoculated peripherally with the street rabies
virus and divided into 3 groups.
The results obtained showed that in,the group
; ;a which extinction of the reflex had, occured, 3/5 of the ani-
mals died the as in the group of control animals; in the
group in which saline injections replaced the chloral hydrate
injections, 2/5 of the animals died of rabies and in the group
in which chloral hydrate injections alternated with saline in-
jections 1/4 of the animals died of rabies.
These data demonstrate the role of the cerebral
of t S U~
FAR
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
158
cortex in the reactivity of the organism and the possibility
of mbdify,ng. theaevolution of infectious diseases by inhibi-
tion of cortical reactivity.
262. ACAD.ST.S.NICOLAU,'N.CONSTANTINESCU, M.DUCA. Investi-
gations on the effect of induced sleep-ih-ex.;.
perimentsl-rabies; Bul Stii.nir;Acad:R:P:R: - _.
SeOt;St:Med;; -1953,' 5, 29-229.
A study was carried out in a series of experi-
ments on rats on the influence of prolonged sleep on the
evolution of experimental rabies.
The results obtained showed that sleep induced
by medinal has a net influence on the evolution of rabies in
rats, prolonging the period of incubation from 11 days in the
controls to 14..4 days and even permitting survival of one of
the lo animals inoculated.
The effect of the state of inhibition is more
obvious in the lea in which sleep preceeds the cerebral in-
fection.
.263. P.STROESCU, I.MIRZA. Modification of higher nervous
activity in miee-inoCaldted-WSth--the^entt.:
rabiee-vaeeSfle:~St:eePe:tSPPeffiICP08Sd~?yffiSCro-
biol:;pat&2ttbl.; 1953, 4-, 3-4, 367.
Investigations were carried out on modifications
of the higher nervous activity in white mice inoculated with
the anti-rabies vaccine, in which conditioned alimentary-motor
reflexes had been previously elicited. After inoculation,
modification of the higher nervous activity takes place in
the first 24-48 hours. These modifications reflect the
process of supraliminary inhibition manifested by a prolonga-
tion of latency, inhibition of o ti
k~r ;, p ei ve cond~,t3.oned reflexes
and enhancing of internal inhibition. Certain non-conditioned
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
(defence and orientation reflexes) are more accen:?
t! .,~ ~. ~d g which indicates a certain liberation of the subcorti-
co 1. regions from under the restricting influence of the cor-
'::a ? od7 fic6tions of the conditioned reflex activity lasts
' 'he mean 16-2o days, after which the reflexes come back
or al.
CONSTANTINESCU9 A.TOMMMA, R0DINUo Excperimental inve stun
, avions on the so called "natural"` immunity.. of
coo , in rabies iffe tion. St.cerc.inframicrobiol.,
micro b lol pa.zazitui-o 1954, 5, 3649 269.
in 5% of the 15o dogs examined, which were re-
f ra-0u L,-, to L epeated inoculations of the rabies virus by ce-
r~v route, no serum rabies antibodies were found, not even
=t? titers, and the nervous tissue did not kill the virus
-; vitro. Since it was possible to immunize the guinea pig
:y ,peated administration of subrabies street virus doses,
tL natural" immunity of dogs was interpreted as revealing
an actively acquired immunity by contact with the
sip:': t: virus in areas with enzootic rabies.
265. N . `OidSTAIVTINESCU9 C.DRAGOMIR, E.DUCA, MLDUCA, GR.TEO-
DOROVICI.- Strains of rabies virus isolated in
Moldavia. Pathogenic activity, fixation capa-
cif and antigenic qualities. Stocerc.infra-
microbiol..,mi.crobi.olm;pa.razitol0, 1954, 5,3--4,
291.
Of the 43 rabies street virus strains - 17 iso-
lated from animals s and 26 from man, - with or without anti-
none, not even those isolated from human
cases considered as due to "unsuccessful treatment" behaved
as `?re .~_r_:fnr.ced" virus strains. The cause of insuccess in the
persons correctly treated with phenol treated vaccine "killed"
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
16o
by heating 72 hours at 37? C was to the method of
vaccination or to individual incapacity of immunogenic reacti-
vity of the organism. The first of these two factors led to
new investigations, following which the classical Fermi vac-
cine was introduced in our country.
~c51 13JInfluence of the street rabies virus
on higher -nerV613_8" acti~rity in whita ? rats. " 5z a cerc.
inframicrobiol.,microbi.oi. parazi.tolo'"1954, 5,
3-4, 359-
Motor-alimentary conditioned reflexes were ela-
borated in rats, after which the animals were inoculated with
the street rabies vi"ru.s. In the first 24--48 hours after i.?o-
culation, evident modifications of the Conditioned reflex acti-
vity appeared, which were not observed in the, controls inocu-
lated with normal brain. This demonstrates that incubation in
rabies is much shorter than was thought, and that the cerebral
cortex reacts before any other organ in this disease. Modifi-
cation of the reflexes points to an early establishment of
supraliminary inhibition processes in cortical activity; in-
crease of the latency period, inhibition of the motor-condi-
tioned reflex, prolongation of feeding time, apparent impro-
vement of differentiation-inhibition. The animals in which
these modifications appeared suddenly died, whereas those in
which they appeared slowly survived inoculation.
267. P.STROESCU, R.PORTOCALA, I.ADERCA, G.DANIELESCU. Resear-
ches on septineuritis-in "closed 'rabies". Stecorc.
iziframicrohio1', smicrobiol~ aparazitol-. -1954, 59
3-4, 4o9.
An experimental histologic study was carried
out on septineuritis in "closed rabies", They investigated
at the same time the virulence and lesions caused by the rabies
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
161
virus inoculated to animals which had been previously incom-
pletely immunized against rabies.
The experiments demonstrated that the rabies vi.ruu.;'
has an initial affinity for the vegetative nervous system, bDtb
peripheral and central, so that the parotid gland, the sympa-
thetic and parasymphatetic nerves are often more virulent than
the peripheral sensitive motor nerves or even the neuraxon.
The anatomo:~gathologic examination shows in the peripheral
vegetative system intense rabies inflammatory processes and
the presence of a great number of Babeg-Negri bodies. The
inflammatory lesions in the neuraxon are to be found at the
level of the vegetative centers, around the 3rd ventricle,
the bulbus or spinal cord. In the spinal ganglia, inflamma-
tory processes of medium intensity and rabic inclusions are
revealed. No apparent lesions can be found in the sensitive---
motor nerves.
In view of the well known role of the vegetative
nervous system in the elaboration of immunity and defence func-
tions of the organism, the difficulty of establishing immunity
in rabies can be explained both in the course of the declared
disease and after anti-rabies vaccination, by the fact that
this system is the most vulnerable to the attack of the rabies
virus.
268. N.CAJAL, S.MATEESCU. Rapid diagnosis of rabies with the
contrast phase microscope. St.cerc.inframicrobiol-g.
microbiol.,parazitol., 19551, 6, 1-2, 35.
Gior.di malattie infettive e parassit.,195?,2-
Since the problem of a rapid diagnosis of rabies
is of paramount importance, particularly with a view to es-
tablisxi.ing the beginning of the antirabies treatment and
since until to-day the methods proposed have, besides advan-
tages, many debatable aspects, the authors proposed to find
a swifter technique of diagnosis. To this end, they centered
upon the method of contrast phase microscopy, which permits
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
162
examination of unstained preparations. The results obtained
were excellent; the interval of time required is extremely
short: from the moment the fragment of neuraxon from the
suspect animal is collected and up to its examination at the
microscope, not more than 15 minutes are necessary. Hot fix-
ation is performed in a lo% formol or Dubosq-Brasil-Bouin
mixture and sectioning is done at the microtome, cutting
2o - 50 /u thick preparations from the frozen products. The
slides are then rapidly passed through absolute alcohol and
toluol and the cover slide is fixed with Canada balsam.
The sections thus prepared are examined under the contrast
phase microscope with an immersion objective, after having
previously identified with a small objective the layers of
Ammon's horn, particularly the central pyramidal layer in
which the Babes-Negri inclusions are to be found in great
numbers. Identification of the Babeg-Negri inclusions is
very easy, eaten for an unexperimented eye and their presence
confirms the diagnosis of rabies.
269. N.CONSTANTINESCU, M.DUCA, E,DUCA, A9CHEPTEA. Heated
or autosterilized experimental paralytic-rabies
i?1 partial,lp mmu,,aized animal,so St?aerc. infra
mi.crobiol$s icrobiolotparaz tol.,1955, 6, l?29
9$
In mice and rats vaccinated against rabies with
the Fermi vaccine or with the "katadinized" vaccine (M.Duca
and E.Duca), the paralytic period of the disease was prolonged
up to 9-16 days instead of l-2 days, as noted in the control
animals. This prolongation of the clinical phenomena allows
the partially immunized organism to defend itself against the
infectious rabies virus, autosterilization of the neuraxon
takes place and the animal sometimes survives ("healed para-
lytic rabies").
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
163
27o. N,CONSTANTINESCU, A.STIRBU, A.CHEPTEA, C.TAINDEL.
The influence of the treatment on antirabies
vaccination before and after infection.
St.cerc.inframicrobiolOmidrobiolo parazitol.,
1955, 6, 3-4, 3750
In preventive vaccination, applied to mice and
rats, according to-the treatment scheme with small doses
of Fermi vaccine (6 doses at 2 days interval, total dose s
0.37 - o.4o g nervous substance per kg body weight) a more
accentuated state of immunity was obtained than with the
intensive treatment (21 daily doses, total dose l0.5 g per
kg body weight).
The intensive treatment, does not completely
inhibit the immuna-genetic process and seems useless and
definitely unfavourable in antirabies vaccination.
271. G.DANIELESCU, L,MIRZA? Modifications of the higher ner-
vous activity in white, mide followin `anti` abies
vaccination and subs. izent inoculation of the ., ,_
stre~ t `rabies- virus St cerc o iriframicrobiolo s
midrobioia9-para2itol., 1955, 6, 1.2, 55?
Investigations were carried out on changes of the
.higher nervous activity in mice, in whom alimentary-motor
conditioned reflexes had been elicited and who were subsequent-
ly vaccinated against rabies and then inoculated with the street
rabies virus. Modifications of the higher nervous activity that
appear after vaccination are very reduced in comparison with
those that appear after inoculation with the non-attenuated
live vjruE...(~ In the first 48 hours after inoculation, a state
of supraliminary inhibition appears I thelconditione4 reflex
activity, manifested by prolongation of latency and duration
of the run for food after applying the positive conditioned
stimulus, prolongation of the necessary time for ingestion of
food and apparent improvement of differentiation-inhibition.
None of these modifications appeared in the controls.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
164
272. L.MIRZA EMINET,,J.SAMUEL, E.NASTAC,-C.IACOB. Conditioned
reflex activity in dogs during vaceinatian"aOinst
and subsequent inoculation with the street rabies.
St.cerc.inframicrobiol.,microbiol.,parazitol.,1955,
6, l-2, 65.
Alimentary salivary conditioned reflexes were
elaborated in 4 dogs, after which the animals were vaccinated
with an antirabies vaccine and 34 days later inoculated with
the street rabies virus. Modification of the conditioned
reflex activity, of the supraliminary inhibition type, appear-
ed 13-22 days later. These modifications appear at the mo-
ment of maximum resistance, checked by biologic tests. They L.
appear more swiftly but more superficially in the choleric
type, more slowly but profoundly in the phlegmatic type. In
the first 2 days after inoculation, an exaggeration of the
excitation process is observed, followed by a process of
supraliminary inhibition: prolonged latency, inhibition of
the positive conditioned reflex and nonrconditioned reflex,
alimentary reflex and apparent improvement of differentiation-
inhibition.
Disturbances of conditioned reflex activity are
more profound in the choleric type, in which they were more
superficial after vaccination, and more superficial in the
flegmatic type, in which they were more profound.
273. N.CAJAL, G.DANESCU-POPESCU. Research.-- on antirabies
immu.ziization in rabbits-with.-modified-te&dti
vity of the central nervous" systeM...St.cere.
inframferobiola,microbiol.,parazitol.,1956,
7, 3, 311; na c;n
gress of Medical Sciences, Comunicarile Sect.
Neurol.,endocrinol., Bucuregti 1957, 27.
In order to study antirabies immunity in animals
whose nervous reactivity was modified by certain drugs in the
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
165
course of the specific vaccination process, benzedrine was
used as a stimulating substance and medinal as an inhibit-
ing substance. Vaccination was obtained with a phenol
treated fixed virus vaccine, prepared according to the
Fermi method.
Twenty-one days after injection of the last
dose of vaccine, the rabbits received a challenge dose by
intramuscular route, with the street rabies virus.
The experiments carried out showed that the
antirabies vaccination of rabbits, performed
with administration of benzedrine, establishes a higher
specific state of resistance than in the controls.
In opposition, medinal administered at the
same time as the-vaccination, is followed by a lower degree
of immunization in comparison with the controls.
These differences may be noted only when a
vaccine with an attenuated virus is used and never with
vaccines in which the virus has been totally inactivated.
274. N.CONSTANTINESCU, A.STIRBU, C.TAINDEL, A.CHEPTEA,
I.STOIAN. Comparative study of the preventive
immunogenic _ -taliie " Of phenol. - attenuated and
killed"antirrabies-vaccines: Arh:romede"epide-
miol:-i-microbial:;"St?ei_Cerc. Edit.Med.,
Bucuregti, 1956, 353.
Comparative study of the immunizing activity
in mice of 4 attenuated or killed vaccines yielded the
following results, regarding the proportion of animals pro-
tected against challenge infection by cerebral route, with
the fixed virus (F) or the street virus (S)
Fermi vaccine =
29%
(F) ;
38%
(F) ;
29%
(F) ;
52%
(s).
Phillipps vaccine =
21%
(F);
16%
(F);
30%
(F);
59%
(S).
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 -
166
Vaccine killed 72
hours at 370 C ll% (F); 0 (F); 0 (F); 25% (S).
Vaccine killed 2
hours at 37# C
32% (S).
Controls 0 (F); 0 (F); 0 (F); 0 (S).
275. NQCONSTANTINESCU, A.STIRBU, A?CHEPTEA, C.TAINDEL.
Study on the comparative protective activity of
the auti,abies ' sePura' atd" of "' fihe~ol" heated..
vaoe.i,ne: .n.. e- pPbim- e ital, St?cetd a infra-
microbi,ol , flmicrobio flpa1^azitoi ,1956, Z, 3-4,
423,., Giornale Malattie infettive e parassito,
1957n 2, 231.
In experiments on 1,408 animals of various spe-
cies, it was.,observed that the antirabies Fermi vaccine does
not confer protection when it is administered after intra-
muscular infection with the street virus. When the immune
serum is administered alone or associated to the Fermi vaccine,
a net protection is obtained resulting in survival or prolon-
gation of the incubation period and paralytic phase of rabies.
These results led to the renewed application in our country
of sero-therapy in human rabies.
276. N.CONSTANTIN.ESCU, N.BIRZU. The process of autosterili-
zation in rabies. Sty,eerG infrainicrebiol ,micro-
biolo,parazitol.,1957, 8, 4, 517. - The National
Congress of Medical Sciences, Comunicarile Sea#?
Neu.rol-,endocrinol.,Bucuregti, 1957, 29?
In animals treated preventively with egfective
antirabies vaccines which died however with the symptoms of
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
167
rabies following challenge inoculation with the fixed viruu>,
by cerebral route, partial. or total (LD50 = o; autosteri.i i ,
zation of the neuraxon occurs. Comparatively, in
r..- ~rar
cinated mice, the titer of the brain is LD5? = lo ' to
10-11._3 6, Some of the vaccinated mice contracted rabies of
long duration, followed by recovery.
277?
The brain of mice with evident rabies para;'_y sa s,
that had been previously vaccinated with a low efficac?y~a.
cine (14% of the animals resistent to the challenge inocu,
lation), yielded the same titers as the untreated animals
which died of rabies (LD5o = approximately to"6"5).: In con-
trast, in the animals vaccinated with the Fermi vaccine (58%
survivals after similar test-infection), aut;osterilizat'ion of
the neuraxon almost constantly occured.
278. ACAD.ST.SJNICOLAU, L.MIRZA-EMTNET, N.CAJAL. Izyesti-
gations ka the role of the hi,&her ae ova -gip :
in experimental" rabies- and.- the- Pkftepg- Ofd
rabies" imnttrtit ~ _ Gto .ale" di~Maiitti.e" i fettive
e parassit;fl_295?, 9fl 2a
-
The res4ts obtained in numerou-is and varied ex
periments, ' carried out with a view to studying; experimental.
rabies by means of Pavlov's methods are described (condii?: icned.
reflexes, modification of-cortical reactivity by chemical and
physical agents, etcg,)
The data obtained clearly show that both in the
case of rabic infection and during the process of antirabi
immunization - immunization manifested by a minor disees
A,~CONSTANTINESCU, N.BIRZU, GR.TEODORO-VICI N.,STAVi1 ..
Prbdess of auto sterilization in rahi~s ; r:.a
tially immunized animals. - Giornale Malatti.e
infettive-e parassit;-1.57, 29 229.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
168
the central nervous system plays an important part. This was
demonstrated particularly by means of the Pavlov techniques,
which are an excellent means for revealing early modifications
of the cortical reactivity caused by infection, modifications
that appear long before any detectable clinical symptomatology.
279. ACAD0ST.S.NICOLAU, RQPORTOCALA. Rabies with delayed incu-
batiOn in vaccinated subjects:" Gibtriale'di-Malattie
it.fettive._ e-patassit?; '1957; - 9, 2, 22o.
The National Congress of Medical Sciences, Comu-
nicarile SecV.Neurol.,endocrinol., 1957, 59?.
Investigations were carried out on 3 cases of hu-
man rabies with delayed incubation (158 days, 738 days and 6
years) which occuraduin inoculated persons. The pathogenicity
of the strains isolated on animals is fairly low.
Microscopic examination of the nervous system in
the 3'cases showed, besides the usual modifications, typical
rabies inclusions in the cytoplasm of the glial cells, white
cells and vascular endothelial cells.
These inclusions were likewise discovered in the
nuclei of the'glial cells in Ammon's horns.
28o. R.PORTOCALA, P.STROESCU, N.CAJAL. Histopathologio study
of the vegetative nervous APteia" ib.. rabies:Gior-
nale di Malattie'infettive`e-patassit:;'1957,9,
2.25.
In the course of rabies, due to the street virus,
the presence of numerous rabies inclusions were revealed in
man and animals in the ganglionic neuron of the retina and in
those of the carotid glomus.
In the partially immunized animals, similar in-
clusions are to be found in the vegetative ganglia, The same
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
169
findings are likewise valid for the nervous intra-parotid
microganglia. The peripheral vagovsympathetic system of
these animals is virulent, which demonstrates that immunity
cannot be obtained without integrity of the vegetative sys-
tem.
It is assumed that the alterations described
explain to a certain extent the vegetative and psychic dis-
turbances encountered in rabies.
281. N.CONSTANTINESCU, N,BIRZU. Investigations on the me-
chanism of autosterili~,atien-..." rabies; ..' " _.
ag
19589 2e 2, 331.
The brain of vaccinated mice completely destroys
the rabies virus in situ or inhibits its multiplication
in vivo. The inhibitory activity explains by itself the
total-or partial process of autosterilization of the neuraxon.
The brain of mice passively immunized by antirabies serum,does
not exhibit this rabicide capacity. Once the virus has pe-
netrated into the neuron, it multiplies - sheltered from the
circulating specific antibodies - as if immunity did not e-
xist.
282. N.CONSTANTINESCU, N.BIRZU. Phenomenon of autosterili-
zation and recovery in.eperimental rabies_
Anzi;de" l1Inst0Pasteut, '1958I _94 - 739....
Antirabies vaccination prolongs the period of
incubation of rabies caused by challenge cerebral infection.
Once rabies has appeared and when the animal is vaccinated
preventively, the autosterilization of the neuraxon almost
constantly occurs. This process of autosterilization may
sometimes lead to "healed paralytic rabies". As a rule, rarief,
behaves in the partially immunized organism like any autso-
sterilizable and curable neuroviral disease.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
17o
283? ST.DRAGANESCU, A.PETRESCU, N,DRAGANESCU. Inclusions
of the Babe .Negri type in a case of - ascending
paralysis not determined etiologically in the
c,iini a Stecerc.neurala 1958, , 2, 161
In a case of ascending encephalo-myelo-'radiculo-n
neuritis, considered clinically of a non=determined primary
viral origin, with albumins-cytologic dissociation and with-
out antecedents of rabies infection, intracytoplasmic Babec?
Negri inclusions were revealed in the cells of the cerebral
cortex,. the Purkinje and Ammon horn.. cells, besides the non-
characteristic infiltrative processes in the nervous system.
The hypothesis of encephalitis with Dawson in-
clusions seim, s very little probable, the virologic diagnosis
remaining that of rabies infection with a clinical character
unusual in the human infection with the street virus.
284? ACAD.ST?S0NICOLAU, N.DRAGANESCU, CLJNICOLAU, N.I.IO-
NESCU. Investigdions on a new type of anti-
rabies vaiiae'with" sil,rer" ihs~ _Staeerc ?in?
framicrobiolo?micrObiblogpaia2itele, 19589
,2v 3, 315.
A new method for preparing the vaccine with
fixed rabies virus, inactivated with the aid of silver ions
is described. This can be obtained by electrical concentra-
tion with silver ions and also by mixing the virulent rabies
suspension with certain silver salts and particularly with
silver nitrate, In a o.15% solution in distilled water, this
salt adequately attenuates the fixed rabies virus. By adding
an aqueous suspension of "silvered" virus, to an equal volume
of 18/boo sodium chloride solutiol, potent antirabies vaccine
is obtained, whose immunizing potential. is at any rate equi-
valent to that of other antirabies vaccines prepared by other
methods.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
171
285. ML.BAB.ES. On the beginnings of Rumanian rabiolo y.
St. cerc: inframicrobi.oL ~ ; " 1959; "Zo ~ " 2; ^ 24T, m
Victor Babes, the founder of Rumanian rabiology,
was the first foreign investigator to be personally initiated
by Pasteur in Paris (January 1886) in the details of anti
rabies vaccination in man.
Babeg's research work at the Pasteur Insta.tu.t,F,
immediately suggested to the Rumanian investigator the idea
of studying the a"gnomonic histopathologic signs of rabies,
the mode in which the rabies virus propagates along the ner?
vous pathways, the method of attenuating the virus by heat,
trepanation of rabbits with the gimlet instead of the crown-
trepan, the swifter transition of the street virus into the
fixed virus by the use of guinea pigs, etc,.
Rumanian rabiology greatly benefited by these
discoveries of Babeg's, already from its beginnings (May 6,
18880)
286. N. CAJAL, G?DANESCU- POPESCU. The evolution of expo
mental rabies and antirabies m~an~f; dhUt- the
action- of-dorti exo; 4-' -The -Sed6hd-Cohgtess of the
Hungarian Microbiologists, Budapest, September
22-=26, 19590
Investigations on hamsters, mice and rabbits,
concerning the influence of cortisone on the evolution of ra-
bies with the fixed virus or the street virus and anti rabies
immunity obtained with the Flury virus, or with a vaccine with
inadtivated fixed "Babes" virus, led to the following conclu-
sions
Administration of cortisone at the beginning of
the experimental rabies infection increases the sensitivity
of the animal to the pathogenic action of the street virus and,
to a certain extent, to that of the fixed rabies viru.sm ?Thus,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
172
the period of incubation and of survival is reduced, the dif-
ferences being much more evident in hamsters, which as a rule
contract rabies of long duration.
AMrabies immunization in rabbits is more ac-
centuated under the action of cortisone although the sero-
neutralizing antibody titer is lower. In this way, the ab-
sence of a parallelism between the degree of inframicrobial
immunity and serum antibodies is again demonstrated.
287. N$CAJAL, G.DANESCU:POPESCU, Investigations on anti, _-._-
rab ,es immunity in rabbits-V& dirlated -With h--the
"11'a jr" strain ? under the infliuence - of - cordsote .
Stocprc.inframicrobiol., 1959,~1O;4; 447:
A study of the degree of immunization induced
in rabbits by the avian adapted "Flury" rabies virus under
the-action of cortisone acetate, and the dynamics of sero-
neutralizing antibodies, showed that immunogenesis is signiw
ficantly accentuated under the action of this suprarenal hor-
mone. The influence is more obvious when cortisone is admi-
nistered 2 days before vaccination and then 2 more days,
coincident with the "Flury" vaccine. The degree of immuniza-
tion is independent of the titer of the serum neutralizing
antibodies, which definitely decrease under the action of the
corticoid hormone.
288. N?CONSTANTINESCU, N?BIRZU, I".MICU, O.ZAVATE, FL.VASOIU.
Process of autosterilization in human rabies.
Stooero;Stiint;;tedicin ;'Acad:R:P:R:"Pil:Ia i,
1959, 1oD 1, 1290
The same process of autosterilization is ob-
served in human rabies as in animals. The cerebral tissue"
in non-vaccinated human cases yielded titers of LDSs =lo .3
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
173
to to 5009 whereas in vaccinated persons, who however deve-
loped rabies, the virulence of the brain only attained
LD = 10-1?3 to 10-2"5? Since the possibility of the inter-
50
vention of a. naturally attenuated strain is excluded, these
low titers reflect the process of autosterilization in vac-
cinated persons.
289. N.CONSTANTINESCU, N.BIRZU. Is not rabiesa curable
disease ? La'Irlesse M6dicale-1959;'7,98 33i
Considerations are made on the autosterilized
or healed rabies infection in naturally resistant or sensi-
tive animal species, on 'inapparent infection, rabies septi-
neuritis and "closed" rabies, This behaviour of the disease
implies the necessity of renouncing - in the case of human
rabies in vaccinated persons - to euthanasia by morphine and
of adopting an adequate symptomatic therapy, as in the bulbar
form :of poliomyelitis.
290. N.CONSTANTINESCU, N.BIRZU.y IJMICU. Research work on
the mechanism of protective-activity of the
antirabies set='I Stocercoinframicrobiol,1959,
lot 3, 297.0
The protective activity of antirabies serum is
conditioned by direct contact between the rabicide antibodies
The virus is neutralized
and the infectious rabies virus,
only as long as it has not penetrated into the Nervous system;
once it has penetrated - the intraneural phase - the action
of the serum becomes nil. These results implye that, in hu-
man therapy, local serum infiltrations should be done directly
in the anatomical region infected, at the same time as general
serotherapy.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
174
291. ACAD.ST.S.NICOLAU, B.FUHRER. Investigations on the
presence of the street"rabi :virui?'it- the"
blood-of a eritnentally' infected' rabbits...
Report-read at-the_'Festive-Sciefitific'Session
of the Institute of Inframicrobiology,.. August
17-18,.1959.
The existence of a period of.viremia in experi-
mental rabies in rabbits and guinea pigs was demonstrated.
This period occurs between the 4th and the 7th
day after inoculation of a virulent suspension by intramuscu-
lar route.
The presence of the rabies virus in the blood
of the animal was demonstrated both histologically and*bio-
logically.,
292. ACAD.ST.S..NICOLAU, L.MIRZA, P.ATHANASIU, AL.PETRESCU.
Is it possible to determine by'meana of'the
conditioned-refie2'>aathOd-the limit of rabies
?iras-variabtlitg-in^formolated-antirabies-
vaccines^?
The experiment was carried out on lo groups of
4 rats each,'with previously elaborated conditioned ref-
lexes. Six groups were vaccinated with the variously atte-
nuated antirabies vaccine (formolated o.5/1,000, 1/1,000,
2/l,ooo, 4/1,ooo, 6/l,ooo, 8/1,ooo) the other groups sarv-
ing as controls for the reflexes, for the virus and for the
0.5/l,ooo amd 8/1,ooo formolated protein. The experiments
showed that no changes occur in the reflexes of,the con-
trols for reflexes, of the controls for non-virulent homo-
logous 0.5/1,00o and 8/l,ooo formolated protein,'and the
rats vaccinated with 8/1,ooo and 6/l,ooo'formolated anti-
rabies vaccine (prepared with homologous protein).
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
175
The' reflexes are-''~however slightly upset in the
rats vaccinated .with- 4/1,:000-- form?lated vaccine and * strongly
disturbed:.'in rats vaccinated- with 2/1, ooo, -1/1, ooo ' and'.-
095/l,oob-formolated vaccine; like-'in the,controls for the
non-attenuated rabies: virus..: -It 1s " known' that'. potent vac-
cines in the-field of-,v.iral.diseas?s contain at: the utmost.
4/l,ooo formol. ,.A.-vaccine with. a.. higher: concentration of-.
formol becomes- jion-immilnizi4g.t,,:.non..,operiktive. It may be'
assumed that ,a higher concentration, . of: formol denaturates.
the. chemical ,,constitution of .the germ.and;. destroys- ito The-
dead virus, is inactive against,conditioned.reflexes. as may
be seen from the .. above,,experiment....,,This supports the-idea-..
,that the met hod- .. , of..conditioned .reflexes is a good experimen-
tal 'scheme =for assessing the degree. of ,viability of a virus '.
contained in .avaccne and.its immunogenic potential
293..' ACAD.ST.S-NICOLAU, N.DRAGANESOU, CL.S.NICOLAU,'N.I.
IONESCU. Investigations on a new -type of
antirabies yaccih6-o-"Report"'read at the"-Second
.Congress, of:-the Hungarian - Microbiologists,
Budapest,,. September . 22' 26,, 1959.;
heavy'metals, an effective antirabies vaccine was prepared
for mice.-and.-rabbits,. with . a. -silver nitrate.. . A lo% sus--
pension ..of fixed rabies .virus, Babe 0-, strain, in a. 0.15% sil.-
ver nitrate solution,...-left ,.24hours at---.room,. temperature -in.
the dark,, -becomes vaccinating and.; confers..to the, white, mouse.
On,.the,basis of the oligo-dynamic. action of
With a view to long-term storage of -the vaccine.
thus prepared, to.the., drained off supernatant ;or after centri-
and the rabbita solid resistance to.ingection;.with.the
fixed.-virus .by,, cerebral route.
way, -_an antirabies .vaccine .is.. obtained with an. efficacy on
mice.and .rabbits ,that lasts more than 3 months.
fugation. an. .equal .volume, of saline, solution is added. In this
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
176
Comparative investigations' on the 'efficacy of
this vaccine and that of the Fermi vaccine have shown its
superiority in mice and rabbits. Dialysis 48 hours against
distilled water at 440C of a suspension of fixed rabies vi-
rus in 0.1.5% silver nitrate solution,,showed that in the
working conditions applied, attenuation of the fixed rabies
virus seems to be an irreversible phenomenon.
In order to study the mode of spread and accumu-
lation of this vaccine in the various tissues of the immu-
nized animals, investigations were carried out with Agll0e
TYie vaccine was prepared in a NO3Ag1 0 solution and the radio-
activity of various organs was then determined,
The results show that the attenuated virus penet-
rates into the nervous tissular system, which it immunizes,
through the homologous sciatic nerve. The conclusion may
therefore be drawn that this vaccine follows the centripetal
route of migration characteristic of the live neurotropic
viruses.
294. ACAD,ST.S.NICOLAU, N.DRAGANESCU, CL.S.NICOLAU, B.FUHRER,
E.GIRD, N.I.IONESCU. Experimental researches
anti.r.abie'~~ac5,.fieo Arta" Viro?
on "silvered"
logica, 195931.91,_"RevoSeioMed.,l959, 81.
An antirabies vaccine, prepared with the fixed
rabies virus attenuated by means of a 0.15 % silver nitrate
solution, was demonstrated to be as effective in mice and
rabbits as other vaccines prepared with the attenuated virus.
Migration in the organism of the radioactive
vaccine prepared with Agl10N0~ was studied. Radioactivity
of the sciatic nerve in the inoculated thigh was higher than
in the 14 other different tissues studied, thus demonstrat-
ing the neurotropic affinity of the attenuated virus contained
in the vaccine, although the latter was not pathogenic for mice
on intracerebral inocu.lati.on. The viability of virus particles
in effective vaccines and the results supporting the views on
tissue immunity in viral infections are then discussed.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
177
295. ACAD.ST.S.NICOLAU, NODRAGANESCU, N.I.IONESCU, M.
ANDREESCU. Investigations on the preservation
of the efficacy of the "silvered" antirabies""
vaccine. Comparative study of the immunogenic
effect of the "silvered " vaccine and Fermi
vacs St.cerc.inframicr.obiol., 1959, lo,
3, 2670
An antirabies vaccine was prepared with o.15
per cent silver nitrate solution. Twenty-four hours after
preparib4 the vaccine, a metallo-protein precipitate is
formed: silver-cerebral substance of rabbits infected with
the "Babeg" fixed rabies virus. By eliminating 24 hours la-
ter the supernatant fluid - by simple draining off or by
centrifugation, and adding an equivalent amount of saline -
an antirabies bvaccine is obtained whose efficacy in mice
and rabbits lasts more than 3 months, which proves, in con-
trast with the results obtained in previous experiments, that
the factor determining decrease of the protective action of
the vaccine is to be found in the supernatant. Chemical de-
terminations showed that to ml supernatant contains o.oo16 gm
silver or o.oo2.5 gm silver nitrate.
The infinitessimal amounts of chlorides and NO3
ions present in the supernatant, denote that the existence
of excess silver ions constitutes the factor that determines
the loss of the efficacy of the virus with time.
Comparative researches on the efficacy of "sil-
ver" vaccine, prepared by eliminating the excess of silver
ions after 24 hours and of the Fermi vaccine, showed the su-
periority of the former. Diilyses during 48 hours against
distilled water at +4U of this fixed rabies virus suspension
in o.15 per cent silver nitrate shows, in the experimental
conditions of the authors, that attenuation of the fixed virus
can be an irreversible phenomenon,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
178
296. ACAD.ST,S.NICOLAU, N.JJRAGANESCU, CL.NICOLAU, N.I.
IONESCU, B.FUHRER' E.GIRD. Experimental inves-
tigations on silver antirabies'-7aecine; .. -ReV,- -
Sci,Med,, 1959,:49-816'
An efficient a t.irabies vaccine for mice and
rabbits was obtained with silver nits?ate in 0.15% concentra-
tion.
With the aid of this vaccine, the ascending
migration of the virus-%raccine along the sciatic nerve to-
wards the central nervous system, which it immunizes, was
demonstrated, by calculation of the infectious activity of
the-various organs.
297, ACAD.ST.S.NICOLAU, N.CONSTANTINESCU, N.BIRZU, O.ZAVATE.
Immunity in rabies appraised by the peritonea',
neutralization tests St.cerc;1nframicrobiols;
l96os"ll--I;'9?,--'Acta Virol.Ung. 196o, 7, 2,
184; 'Biull.Nauc.inform. 196o, 2, 131.
The correlation between rabies antibodies and
the evolution of rabies was investigated,* substituting the
classical neutralization test by the "peritoneal neutraliza-
tion" test, To this end, the animals (hamsters, rats and
mice) immunized against rabies with the Fermi vaccine and
which had finished the series of vaccinating injections
1 to 163 days previously, received by peritoneal route a
10-2 suspension of cerebral substance from rabbits infected
with the fixed Babel rabies virus. After 6o to 9o minutes,
the peritoneal fluid was sterilly collected by puncture and
inoculated intracerebrally to 5 mice.. The state of immunity
was appraised by survival of the animals in the experimental
group. The results obtained demonstrate that in comparison
with the animals of the control group, that died of rabies
encephalitis, "the peritoneal neutralization test" is very
precise in showing the state of antirabies immunity and the
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
decli.ne of immunity,
G s
",i alss af trer
boos, t? r .o
re i.~.7,~e?.lo'ii'-:'.~.[4'.,. f.,7'r.`~.~,? - .A.!:l ~..
~m'ad~e evident o:~" I__7
of the 'Vr .rus.-.
298'. 1VoCONSI'ANTINESCtT9 "'N.CAJ'AL9 ' N..BZRZU9 M.CEPLEANU', 0
ZAVATE. Experimental rabies infects in the
Syrian hamster: St. cerc. i lfran~icrobio1.196o"
11,`' 1, 61^; Adam Virol.Ung.196o,- 7,,
2, 184.
0 L,
Study"o `t`he evolution of experimental rabies
infection in the Syrian hamster,showed. that this - animal .. -
species ' is ' vary 'receptive both to the `street and the fixed
rabies virus. ' In -contrast"'to other sensitive animala, the
to 20 days.
paralytic phase in the hamster is exceptionally long, up
bodies in loo per cent of. theneurons, with a characteristic
internal structure --;instreet rabies virus; numerous in-
clusions-of-variable size;:formed - of_ homogenous oxyphil
mass, situated in, _ the,.. neurons of Ammon''s horm- and --of' the ~.
hippocampus :in, fixed "Babel",..virus.rabies.
ticularly intenseilarge and. sometimes giant Babe?-Negri
-By'prolonging the conflict - between the virus
and the organism, a process*of"autosterilization develops
in a number: 'of 'a'nimals. similar to that observed in other
laboratory 'animals' partially` immunized by antirabies vaccina-
tion. In ~this-species, " the inclusiogenetic process is -par-
x.79
: ;y' r..n hamsters and rats.
,:Grua.: l: -' attenudteo byy long inter-
t,_ .'s:o9 m_ y e r ~. t ;:vated by
'tralization. test
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
299. C.SURDAN, E.DUMITRIU-CAROL, C.CURE, M.ANGELESCU.
Contributions to the study of post rabies vacci-
nation neuroparalytic complications in the*doG?
St
virus which does not
cans ,
self or because it
immunized sub jec .s,
tion is, as a rule,
bo
d
wes, may be absent, either
s i., e case is due to an "enhanced"
u' e appearance of inclusions by it-
e in... vaccinated, i.e. partially
1 -,hieh the process of inclusion forma-
i_:ryhibited in its development"
3o8. I.:BERLOG.EA, I.ST TI, &SCHOENFELD, NOCAJAL, G.POPESCU.
1' , e t ; dns on rabiess infectjo . and rabie3
a-m,-,r? ty in an imaIa subjected . to irradiatioh,'
SL1G~:;rrt'i'X3.fa.`IiI,.`~p16.1 f'
In e- p riwents performed in white mice and white
rats, i--rrad i atior. of the animals with 450-5oo r was found
not to alter their susceptibility to the inoculation of va-
r? ous rabies strains ("Babeg" and "Pasteur" fixed rabies
V ;..ru e-, s e: ^.?e.L ?'iru 9
"Flury" virus)
o
Consequent to the introduction of "Flury" virus,
resistance to rabies is slower to set in irradiated animals.
at the :~n:1- of 20 days, it will not differ from that
ob rci ed. Ic. non.- irradiated animals.
A .- I RhVICIq ml, --SICULESCU, M? GRUTAO Benign lyan.phoo tic
menin itis~ Experimentation oi.' an n.a soStoeePc.
iui?ra:aicrobio1 9 M11950; ..ls '2; I.oSw -
Th.e etiologic agent of certain non=-bacterial
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
meningitides: is investigated. Fourteencase s, exhibiting the`:
Colentina? Clinic of Contagious Diseases.
symptomatology of lymphocytic meningitis-,wee-,-studied in the
-at ona "' ~7Sse ~ s' mittahle i i serf"4l1 passagesby
In the experiment, 122'guinea pigs, 64 mice and
1;2.,rabb is were sed:o From the cerebro. spinal fluid and the
blood, of the ,pat ents~;a causative. agent wasp isolated,;. capable
of .nd cirwg ,~ 'hair- disease: in: g . nea i s' on~.6 of;;the?p.
case S. after' 6cillitioh and in 261o
the , ws s s ., .:~..Lce- ret aee.d 'the 1:8=_'P6tb:.' 3ay:,~after inoc'u`=
t.,_
a; T. ~,:RtTIA The ~- k
3 ~ ? ' ~l . ~ , ?,~ -,:,~;SCU Q i T . - -~ - grope rt"e s-
lung triturates.
of the etiologic .gent of lymfihbeytic-neningitis?
St corca LT c robjol, 195o; 1, 2, 155.
ai'rir.fl _..s., .~-n2$ "7T'
The authors investigate the portal.. of entry
and route of propagation of the benign lymphocytic meni.n-
gitis virus, isolated by the authors, who demonstrate the
role of the mouse as vector of the. disease, this animal be-
ing found in the house of 24 of the 28 cases investigated..
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
l91
317. IpSA!i~NEL. Cho.rc me.:i it: s strains isolated in . : lea s
in 1954 10;2350- C omuniearile Acad. R,P.R. , l956 - .. "
6, 5, ?.3 ;.
Du r. t.. ? g t. h c;
period, 8 meningitis strains
were isolated by _io,c,ulation to mice of cerebro-
spinal fluid drawn from .9 ca ~:. es with a clinical aspect of
benign meningitis. The possibility of breeding contamination
among mice was excluded. Except for a single strain, isolated
in July, all the other strains were isolated in autumn 1954
and 1955. The clinical aspect of the disease, the symptoma-
tology of the experimental infection and the anatomo-pathologic
picture of the animals which died following this infection, are
those of a benign lymphocytic chorio-meningitis, of the Arm-
strong type.
318. I.SAMJEL? Eti?logic researches in viral meningitis,
Gioth;M&iatti.e`itfet'Give"e"parassitQ; 1957, 2.9
An attempt was made to isolate the etiologic agent
from 115 patients with a clinical diagnosis of aaeptic menin-
gitis.
It was possible to isolate 11 strains of chorio-
meningitis virus by inoculation of the cerebro _3-'spinal fluid
to white mice. The isolated strains were obtained from 9 adults,
-. adolescent and a child , in whose homes the existence of
mice had been observed.
In another 3 cases (an adult and 2 children), the
Coxsackie virus was found in the feces of the patients.
Four strains of mumps virus were isolated by ino-
culation of the cerebro-spinal fluid into L-,:he am_rictin' Mav y o :f
7'-day chick embryos. One of these strains was obtained from
the cerebro-spinal fluid of a child suffering from mumps,
without any clinical signs of meningitis, but with spinal
fluid reaction.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
192
319. N.DR.AGANESCU? Study mrf an i,: fra!nicrobi,al germ isolated
from a ease ..f''~i,gn?~~' Ltf'F-.wti~'Sst~fli?2;1ti.So
S.t.cer-c- .. ram icr oyd6I ~Az'i.br6bi6l,; 'b&ir&ZitoIo,
1958 39 -L
L
6 .L eir(.,SMed., 1.958, 3, 2(
An inframicrobial germ was isolated on embryonated
eggs, from the ce.rebro-spinal fl.aaid. of a patient with multi-
recurrent meningitis.
From the of its characteristics, this
inframicrobial germ: .:..sated, like the M C virus described
by Mollaret and Cateigr.e, only on the chorio-allantoic membrane
of embryonated eggs.
Inoculation of the cerebro-spinal fluid by intra-
cerebral route and the scarified cornea of the rabbit, as well
as intracerebral inooU.lat_or to mice and guinea pigs, were ne-
gative, woich rules out the possibility of a chorio-meningitis
or herpes virus.
The isolated virus did not have hemagglutinating
properties, but induced intracytoplasmic inclusions in the
layers of the chorio-allantoic membrane.
The embryos hatching from the eggs inoculated
by intraamniotic route, exhibited cerebral hemorrhagic le-
sions without the presence of inclusions.
32o. ST.DRAGA ESCU, N.DRAGANESCU, M.I.POILICI. Acute primary
choreiform'encephalitis with inclusions; Presse'
Med ,..1958; 66.48-..1693_..
A case of acute primary choreiform encephalitis
ending in death 28 days after the onset is studied. The morpho-
logic examination of the brain showed only discrete lesions
at the level of the white substance (lipid di.;integration,
slight perivenous glial proliferation).
By means of the Mann staining method, typical
intranuclear and intracytoplasmic inclusions were revealed
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
193
L n 'the nervous cells and famphophilr corpuscular matk Pons in
the filial nucleiL. -The presenae-.:of these inclusions showed the
viral origin of this encephalitis, the nature of which was
not determined,.
321. ST.DRAGANESCU, D..SARATEANU, N.DRAGANESCUr Gamvlement.
fixation; with' the tick-borne jvirus> tCcPraga -antt
.mammon- No e )l in Ygariaus:,forms of,~-panenccephaalr t.44s::- Neurol.,
psY ihi.,~ neurochir. 1958,-2--163-,,----
The results of investigations carried out on the
-.omplement-fixation test with the sera of 27 patients suf-
r. from various forms of panencophalitis and from ~8 iaps?
V~ : ently heiallthy ?persons, from the .ho;c:ality 1,n wh1ich :,-cases
of encephal it.is ' ha'd .occured 2 years previously, are presented.
In 4 of the 14 cases --of p.anenq+ephaliti~s in various stages
of the dis'e-arm, ;.positive complement fixation reactions were
obtained with the -sera collected .1-3 months .after the onset
of the affection. The complement fixation reaction -was posi-
tive in a single ,c~awsgj. ft ut : .1'ateva=1 scl:.er.o!s s) -of .the 13
napes of per3, hk aFl pane. .^ 4,a~l :t.is -studied semolog tally.
Fn 3 of the ~$ =a~ppa~r-entbl~y phe:aalthy' c.a.sees,, the complement f ix-
r,.. n .react:%an was positive-*
ST*DRAGANESCU, I.VOINESCU., N.DRAGANES.CU.. P .ch? o Ic
enceephaa-lit s,. Isolation: of .,the, ytirus-,-~Repc:rt read
at the Session;-:of" the".Society .bf`Medical ,S.cfiences,
Section of Neurology,:Bu4ll,a:cosrV,, yS%p.tember :24,1958.
A clinical case of psychotic encephalitis with
a favourable evolution is described, The disease began in
April 1958, with anxiety, head-ache and fever. The patient
was hospitalized a few days after the appearance of the first
symptoms in a psychiatric ward, where a diagnosis of schizoid
syndrome was made. In hospital, the patient exhibited a mode-
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
194
rate subfeb.L iL: state, with a fever-peak of 4o.20 C and a
convulsive fit. From the neurologic point of view, slight
meningeal phenomena were observed, paresis of both m.rectus
externus, a right facial paresis of central type, slight
modifivation- of the osteo-tendinous reflexes and motor de-
ficiency. Moreover, the patient was confused, with slight
desorientation and catatonic tendencies, Intracerebral ino-
culations to white mice of the cerebro-spinal fluid withdrawn
in the regressive phase of the disease determined in this
animal species . ?:L_Lv - ...,,-siomena of encephalitis, serially
transmissible.
7.23,, ST.DUGANESCU, S.VOINESCU, A.PETRESCU,' N.DRAGANESCU.
Diffuse neoformation process with encephalitic
.~e r
aspects, St?ce~c;ne Oi:, 958; 4;"375:
A case clinically labelled as pseudo-tumoral
encephalitis is described. The morpho-pathologic examinadion
d diffuse sarcomatosis Of the cerebral blood verse
actual perivascular sarcomatous encephalitis proses;
he various regions of the brain, gaving however a t- o a:.
:hickness in the subthalamopeduncular area. Cytologic study
the infiltrative processes in the meninges and nervous
parenchyma showed polymorphism and the character of the va-
rious tumoral cells. On the sections, stained by means of
Mann method, numerous eosinophil inclusion- like- form
Nations of various sizes, some of them with a halo around
them , were observed within the nuclei of the tumoral cells.
These are chromatic formations, since they are positively
stained by the Feulgen method.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
195
32!~a
belonging to the arthropod borne grvapIdea-
tification of the causal agent and study of
the nervous.lesions inducedin the white mouse
St.eerc..inframicrobiol@,1959, lo, 3, 3630
An encephalitogenic inframicrobial germ, patho-
geaie for the white mouse was isolated from the cerebro-
pinal fluid of a patient from the Dobrogea, admitted to the
,11-nic with phenomeiia of psychotic encephalitis.
It was proved by the sero-neutralization test
against.the standard encephalitis serum (strain Hy PR),
that the isolated germ belonged to the group of tiok-bonne
;._ . ahalit ide s .
Auatomo-pathologic examination of the brain of
the mice whioh died following the experimental-disease showed
a pawn-encephalitis, characterized by a process of perivascu-
, inifF I t:;-.-at' ion, glial proliferation and neuron ophagic no-
dules.
N,DRAGAIVRSCU, inframierobial meningo-encenhalitiiss
N?DRAG ;r ' 3OTY. Virological determination of spring
summer tick-borne encephalitis. in the Rem
perime ntal researches on isolated strain.
Report read at the Second Congress of Hungarian
Microbiologists, Budapest, September 22-26,1959?
An encephalitogenic 'inframicrobial germ, patho-
g-nio for the white mouse, was isolated from the aerebro-
spinal fluid of a patient from the Dobrogea, hospitalized in
the clinic, with phe:omena of psychotic encephalitis. The
sero-neutralization test in mice against the standard ence-
phalitis serum (strain Hy PR) showed that the isolated germ
belonged to the group of Russian tick-borne encephalitis vi-
Anatomo-pathologic..examination of the brain of
the mouse, which had died following the experimental disease,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
196
showed that it was an ence,~,.aalitis charac Lerised by a pro-
cess of perivascular infiltration, glial proliferation s,^l
neuronophagic nodules.
Inoculation of the virus by respiratory route.
to mice induced,,, both clinically and morcho-pathologically,
pneumo-encephalitis lesions, characterized by interstitial
lesions at thelevel of the lung and by inflammatory le-
sions throughout the neuraxon, specific of this viral di-
sease.
Four serial Intrapulmonary passages multipli4d
this virus in the pulmonary tissue, without giving it how-
ever strictly pneumotropic properties.
Intracerebral inoculation to the young and adult
hamster determined clinical phenomena oi'encephalitis, cha-
racterizad by paralysis of the hind quarters, without con-
vulsive tonico-~clonie fits or muscular fibrillation.
The anatomo-pathologic lesions are much more
intense than in the white mouse and are characterized by a
meningo-encephalitis process.
326. N.OAJAL, Y.COPELOVICI, M.CEPLEANU. The action of
amniotic.and all.antoic.fluids of birds on the .
MM encephalomyocarditis virus. Lucrarile celei
de a 11-a Sesiuni pt. a I.M.F. Buc., 13-15 april.
1956, 91.
In view of the results obtained with the influenza
virus, investigations were carried out on 362 mice in order
to establish if the amniotic and allantoic fluids can be used
as diluents also in experiments with the MM encephalomyocar-
ditis virus.
After inoculation of the virus by intramuscular
route, it was found, as in the case of the influenza virus,
that amniotic and allantoic fluids - especially the former -
produce an increase in the virulence of MM virus, assessed
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
197
by the LDS0 in comparison with dilutions in r r,1 saline.
The mechanism of production of the differences
observed appear to reside in protection of the inoculated
elementary bodies by the embryo fluids against the ithmediate
defence action of the organism.
X27. L.MIRZA-3MINRT, I.SAMURL. Cortical reactivity in case
of ininap arent infection of the MM type inwhite
rats.. St.care.inframicrobiol.;microbiol.*,para=
zitol., 1956, 2, 3-4, 327.
An attempt was made to detect i apparent in
tti43tion of the MM type, with the aid of conditioned reflexes.
Oka this end, alimentary motor. conditioned flexes were ela-
borated in a group of 2o rats, after which the animals were
inoculated with the encephalomyocarditis MM virus. Only 2
rats exhibited transitory paresis, no evidence of the disease
being observed in the rest of the animals. However, signi-
ficant modifications were observed in their conditioned ref-
lex activity during the first 24 hours s prolongation of la-
tency, inhibition of the motor conditioned reflex and non-
)nd.itioned alimentary reflex and an apparent reinforcement
of differentiation-inhibition. These disturbances did not
;.pear in the controls, which meant that although the cli-
nical signs of the disease were likewise absent, the animal
4P,d contracted an inapparent disease, detectable by means of
the method of conditioned reflexes.
328. 1.ADBRCA, M.IANCONNSC1T. Cultivation of the MM enceuhalo-
_ Ids m-..o?~
myoeardiac virus in roller-tubes; St;oorooinfra-
m.icrobiol.,miarobiol.,p&razitol:,1957, 9919 49,
MM encephalo-eyocardiac virus was cultivated on
neuronal tissue cultures of mouse embryo, by the roller-tube
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
198
~.etho0 `?'r> virus constantly produced a cytopathic effect,
in the cultures of proliferative cells, Six serial passages
were carried through with a theoretical end dilution of
5xle 100
The attempts made at washing the virus off the
cells, showed that it is constantly adsorbed within 15 minutes,,
the washing not preventing the action of the virus on the ce i l'i.
Tntracerebral passage in mice of the culture fluids from each
passage showed a decrease `?a titer due probably to attenua-
tion of the neYIRZA . Cortical reactivity in anti-influenza immu-
nity processes.._- Report read at the' Society of Medi-
cal Sciences,Bucharest, October 19,1956:
One of the:several aspects of immunogenesis processes,
was investigated,i.e. the reactivity of the cerebral cortex
during anti-influenza immunity. The experiment was carried
out on a group of 3o rats. Cortical reactivity was studied
by means of conditioned reflexes. When the reflexes were
well established, the animals were vaccinated with the an-
ti-influenza vaccine. In the first 24-48 hours after vacci-
nation, a powerful state of supraliminary inhibition was ob-
served in the conditioned reflex activity. Periodically the
reflexes came back to normal, an inhibition--phase appearing
soon afterwards, the conditioned reflex activity thus taking
on an ondulating aspect. A parallel study of this activity
and of the titer of antibodies showed that this titer did
not increase to the same extent in all the animals and that
there existed a strict parallelism between this factor and
the severity of the disturbances of conditioned reflex ac-
tivity.
372.- D.SARATEANU, I.NICOARA, EIZICOVICI, T.VIRF, COVACI.
Dynamics of s6ru .anti-infl uenza- antibodies in--vacci-
nated persons in the- tbWa'- bf--
le Soc. Stun '.fined:. T~:ffiti re$" 'ebraary 8,-19
9 56.
The results of serologic investigations in 277 cases
of vaccinated persons and 266 controls are reported.
An increase of anti-influenza antibodies is observed
for all virus types (A, A', B), the increase being higher
for type A and lower-for types At and B.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
?227-
The highest antibody titer was noted 2 weeks after
vaccination, decrease taking place approximately 2 months
after vaccination.
Although epidemiologic tests were not. carried out,
the authors conclude that in view of the innocuousness of
the vaccine, prophylactic vaccination should be recomended
as the: most effective method for the control of influenza,
373.- DaTARCHILA, CH'MARINESCU Laboratory.diagnosis-.-Gf
the severe forms of influenza in' infan.tsq O.i S.r1. -
'~_onal Conference of Pediatrics; - Fehr ua31-8~1o e1956, 257 ?
The laboratory diagnosis of influenza in small infants
is of great practical use, particularly in the severe and
atypical forms. Difficulties are however encountered due to
the particular serologic reaction of the small child and to
the collecting of.pathologic products at this age.. The la-
boratory diagnosis of influenza in the small infant'is ba-
sed upon specific elements ( the causal agent and serologic
reactions) and nonspecific elements (le ogram,etc.),
The presence of the causal agent can be detected by
morphologic changes in the naso-~-pharyngeal epithelium (rhi-
nocytogram and pharyngocytogram), by inclusions,: elementary
bodies and finally by identification of the influenza virus,
ixr"various pathologic products (naso-.pharyngeal washings.,
_Vates, 'blood, Scerebrospinal fl td, urine, and fragments of,
viscera in lethal cases) and their inoculation to sensitive
animals (mice, ferrets); Specific antibodies are revealed
by hemagglutinoinhibition, sero.aeu 9l ~t bz#s- a Anplement
.fixations, and precipitation on llc9~~ T~tc &p
The nonspecific elements of diagnosis are: the leuko-
gram, sedimentation rate, cold agglutination and certain me-c
tabolic disturbances that appear in the course of influenza-
The above procedures are described in detail, special
stress being laid on the value of repeated hemagglutinoin-
hibition,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
- 228 -
374.- M.VOICULESCU, MaRADULESCU, P.MICHEL, I-.SAMUEL.
The incidence of influenza etiology in atypical pneu-
monias. Second 1 oiahtific Session of the Medico-
Pharmaceutical Institute,BDabarest, April 1956, 184.
In the absence of influenza epidemics, investigations
were carried out over a period of 8 months, on the inciden-
ce of influenza etiology in atypical pneumonias - and a
proportion of 29.1 % influenza pneumonias, was found, iden
tified by means of the Hirst test.
The clinical forms were medium and slight. The data
obtained demonstrate maintainance of influenza in'the inter-
epidemic periods.
375.- A.B$QN TKI, A, 'ET.REEEffi, PPETRP:SCUU, P,ATHANASIU-
STROESCU'.-. Isolation- of an - influenza- strain with pre-
dominant netirotropie "' character - from.- an- itfaiitiX lethal
c e; ". St: eetc e, ihfra ni crobiol.: " mierobiol s .. parazitol
1957, 8, 4, 561.
A new influenza virus strain was isolated from a su-
praacute formcof influenza, ending in death of the infant,
and adapted to the white mouse by intracerebral and intra-
pulmonary inoculations of trachea and intestine. The presen-
ce of the influenza virus was initially demonstrated by a
positive serologic hemagglutinatio:,ition reaction, with
serum standard A' from the pathologic products (intestine,
trachea), taken from this case. The immune sera prepared
on rats'and rabbits confirmed the antigenic character of
strain A'.
During passages by different routes (lung, brain) the
strain exhibited predominant neurotropic properties, which
were constantly maintained during the experiments. These pro-.
perties were revealed by a shorter period of adaptation, ra=pid death of the inoculated mice, a more constant positive
Hirst reaction and in higher titers for the strain main-
tained by cerebral route as compared to the strain maintai-
ned by pulmonary route.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
Theanatomo-pathologic lesions caused by the strain
. i
oculated by intracerebral and pulmonary rou.
n
under study,, to shove the presence of the same pathogen# agent, determi-
ning influenza lesions with similar characters,iin which the
vascular element is prevalent. The toxic effects
strain brought about clinical manifestations (convulsions)
and anatomo-pathologic manifestations characterized by by-
peremia of the internal organs.
37th:- N:;CAJAL, t4.MANOLIU, N,4DRAGANESCU. Evolution of ex-
14 t lar ac- -- '
t
t
?
e
rea
,
pe-e
rim rital influ n
nir o .,957~_f 9561
St?ce ~x__a r ex
erimental influenza in
p
mice., prolongs the period of incubation of the disease and
slightly bwers the mortality rate.
nronhglaxis in. t}~?
377.- A.DEREVICI. Prenatal antiinfluenz
Report
.-.
s vaccine.
autecht -~
n
R.P. Re carried Wit-With a rea R.at~t .a'Se sich~ofth'O -.InstitC t 8f- ISf robi.t--
logy of the R.P.R. Academy, - September
rder to determine the best period of vaccination
t
o
, n
for pregnant women, with a view to protecting new-born in-
fants against influenza, investations were carried out on
the anti influenza HAI antibodies in 57 pregnant women, du-
ring the last 2-3 months of pregnancy, vaccinated with an
A nine=fold increase
autochtonous anti-influenza ooovaccine.,,
in the titer of these antibodies is observed two weeks after
vaccination; two months after vaccination, the titers begin
to decrease, presenting only sixfold values and returning
almost to the initial titer after about 3 1/2 monthmS. In the
group of pregnant women which had rece3lMd apprQx ately 2
values
months previously tetanie anatoxin,, higher antibody
were obtained than in the women vaccinated only against in-
fluenza,
As regards the correlation between, maternal antibodies
and those of r the new-born infants, examined.. in threw` diffe-
ent groups, according to the interval between vaecination
and parturition, it results that for each .group a fairly
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 - -
- 23o
close coincidence. exists between the titers-of the mater a1
antibodies and those of the child, It likewise resulted that
the best period of vaccination for pregnant women, with a
view to protecting the.child, is approximately 1o +5 days
before parturiton,
378.x- L?DEREVICI, A?BRONIT$I,. A?PETRESCU. ~_nvesti ations
on - the robl-em- of influenza virus caff`"Rep
read at the Session of the Institate,_?f_,infraiorobio-
logy of the RbP. R. Academy,-April
29, 1957.
In view of isolating the influenza virus in non-epide-
mic periods, hemagglutination tests were performed with the
nano--pharyngeal washings of two. groups of
,without respiratory manifestations. persons, with or
In the first eategory ewithout respiratory manifestatie
ons ,. which included 12o persons, hemagglutinatioza was nega
tive in the naso-pharyngeal washings examined directly; 8i-
milar results were obtained with the allantoic fluids from
eggs inoculated with these washings, submitted to concentra-
tion with human red blood cells (3-5 passages),
In the second group of patients with resPirato
-
ry manifestations forming par, of various collectivities with a
total of 1,9oo persons, only 3 positive hem;a lutinations
were obtained with the naso-
pharyngeal wa'shi,so
Inoculation to embryonated eggs of some"of the naso-
phaXyngeal washings yielded negative hemaglutination reac-
tions,
Serum hemsgglutinating antibodies were
positive in 8
patients with respiratory manifestations;* dynamic increase
was observed (3-4 fold) in three of these patients, which
made it possible to establish the influenza etiology although
it was not possible to isolate the influenza virus. g
It is probable that the vixM, from the naso-pharyngeal
washings is masked during the mon epic to periods or that
the phenomenon of reciprocal Interferenoo intervenes.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
231
3790- A?DEREVICI, AL0BRONITKIo Application of a cytol
a i 1 the
method fnr the rapid diagnosis of influenz
9
errors applied the Cateigne cytologic method in
ct
idemic in the RAP?R Report react at,
.4 0
the Session of'the Institute of Inframicrobiology
of the R. P. R o Ac ademy, July 18,, 19570
~.~
The a
the examination of tracheal washings in mice inoculated with
the products from persons suspect of influenzae
The cytologic aspects, specific of influenza, found in
the cells of the respiratory tract of the mice, 48-72 hours
after infection, make it possible to extend this simple and
rapid method to the speciality laboratories in view of ob-
taining an accurate and swift diagnosis of influenzae
I t,PETRESCU9 AoBRONITKIfl DQSARATEANU, N.
3004 A~AEREVIG9 e
DRAGANESCUo Comparative stud of the d namics of se-
r,?~a are?i~~?.nflue A-Af Trod es &td" of. .nfluerLza strains
. _ _ 6t. ceros
,
a~xifnainidro'oiol,o g icrob Ol parazitola, 19579
The titer of serum HAI antibodies was investigated at
three months interval, by means of survey testse Comparison
of the mean values showed that the curves of antibodies A
obtained in 195`5?- 1955 showed a disjunction between the
curves of antibodies A' ,which 'ran parallel durng the two
years of investigation, and the curves of antibodies B.
which ran, parallel at the beginning, but showed a marked.
1()55, partie*a-
to rise during the last quarter of
ency
tendlarly in the towns close to the USSR, where as influenza c
type B epidemic was reported.
A comparative study of the strains isolated during
twa years likewise showed that there exists no diffe-
virzlenoe
~,_. between them as regards ad.aptabilit
for the number of pathologic prodizt isolated was
however greater in 1955. The isolated strains belonged to
t5 vpe AY, having also antigenic AO f ormati with the excepa-
`iJ of one strain type A? which exhibits4 prevalent near.
+:ropic plopertieso
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
-232-
381.- A..DEREVICI, A?PETRESCU, A.BRONITKI.. Variability in
nature of the influenza virus strains studied in the
R..P..R,e, in the 1953 ? 1955 period. St.cerc.inframicro?
biol., microbiol., para2it6is-l957, 8, 2, 173-
A comparative study of the influenza virus strains
isolated in the R?P.R. in 1953, 1954, 1955 and these strains
and the standard A/PR8, A'/FM 1 and B/Lee strains was car-
ried out.
The strains present a complex antigenic structure be-
longing prevalently to type A. but having also fractions A'
and B, more accentuated in the group of strains iaolated in
1954. The biologic properties (adaptability to mice, virulen-
ce, interval of time until death of the animal supervenes)
were more evident in the strains isolated during the epidemic
period, as compared to those isolated during the inter~epidem
mic period.
Investigation of the evolution (every 3 months) of the
serum HAI antibody titers showed that this titer, which re-
flects the anti-influenza reactor rate of the population, was
modified paralla,. to variation of the antigenic structure of
the isolated strain.
3820.- A.DEREVICI, A.EETRESCU, A.BRONITKI, D.SARATEANU., Biolo-
gic characteristics of the influenza virus straihb
isolated in the RoP. R.. in 1956 St. sere ? iiifra~nioro-
biol?, microbiole,"paraLitol~ 1957, 8, 3, 34.9E
The biologic properties of the influenza virus strains
isolated in the R.P.R. in 1956, are investigated in comparison
with those found in the previous years, showing that from
the points of view of adaptability, induced morbidity, viru-
lence, HA potential and induced histopathologic lesions, the
,strains isolated in 1956 resemble those isolated in non-epi-
demic periods and differ from the strains isolated in the
epidemic period by a decrease of the biologic properties in-
vestigatedm
Dynamics of the serum HAI antibodies chow an increase
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
233
for type A. corresponding to type A of the strains isolated
in fieldwork, which demonstrates that immunization of the
pop. ation takes place especially against the predominant
strain.
Antibodies A? give very low indexes, reflecting the
disappearance of the A' fraction from the antigenic complex
of the previously isolated strains The reactor rate to virus
B shows a significant increase, more'evident than in 1955,
particularly in the towns in the North--Last of the country,
close to the USSR, in which type B epidemics were reported.
383,x- A.DEREVICI, D.S:RATEANU, AoPETRESCU, A?aBRONITKI?
Laboratory investigations on the 1957 ZOfluenza epide?
mic - in the R.P.R-?, tt., cere. inframicrobiol.?, microbiol?,
parazitolos l957, 8, 4, 507?
During the summer influenza epidemic, a series of strains
belonging to type A, having antigenic fractions common with
the strains isolated in the R,.P?R. during the 1953-1954 epide-
mic and with strain A (Asia) 1957 were isolated on embryonated
eggs. These investigations showed the absence of a good a.dap-
tation'of the isolated strains to mice, it being necessary
to passage the mouse products on embryonated eggs in order to
establish the presence of the virus..
Serologic investigations in patients during the acute
phase of the disease and during convalescence revealed a dy-
namic increase of serum HAI antibodies, especially for the
A/PR8 strain, as compared to the 1957 strain (Asia),
The leukocyte counts performed in the same patients
during'the acute phase, showed a smaller number of *hite
blood cells (an average 5,o69 elements/cmm) than during con-
yal_e_sc&poe (an average 9 elements/cmm) and an increase of
%.
of 22 - 44
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
and staphilococcus),, by intr4.asai or intracerebral route,,
in various dilutions,oti
-Hemorrhagic and, degenerative lesions were frequently
tbsued in the toxic forms of influenza in mice and baby-
mice B, inoculated wi~h large doses of influenza virus,, whe-
reas the inflammatory reactions (perivascular infiltrationg'
gliesis) were.observed in the purely nervous forms,, in the
forms associated to pulmonary lesions and in mice inoculated
with the influenza virus associated to bacteria4 Perivasou-
lar-and peri:cgllular oedema was observed especially in baby-
mice?. The.path?genic mechanism of nervous -manifestations in
most` influenza cases in breast-4fed and_..small. infants is an-
counted for by the direct toxic *Si.q~% of the influenza vi-
rus upon the capillary endothelium at first, and then upon
the nervous cell; anoxemia following vaaeul r disturbances
contributes to the seconda nervous sgns Multiplication
of the-influenza-virus in the nervous gel1 or actual neuro-
tropism has not yet been demonstrated, although it scams
possible.0
path.bgenic,bacteria (haemophiius influenzae, pneumococc
234 o
3840- GJMARINESCU? On meningoencephalitis of influenza
origin-. and experimental investi-
gations'% The Third Congress of Clinical Biology,
Bruxelles, July ]A--2ool9510
An anatomo-pathologic study of the central nervous
system was carried out on 25 breast-fed and small infants
with nervous manifestations,,, that died of various forms of
.nfluenza,,and on 232 mice ands rats of various ages, inocu-
lated with a pne.umotropj~o~ influenza virus on the one hand,,
and on the other with the sam.e virus associated to certain
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
? 235 -
3854- I;.MIRZA EMINET, A.PICOS, AL.PETRESCU, AL.BRONITXI.
The role of cortical reactivity in the course of anti-
influenza immunization in white mice:., St:.cerc.:inframi-
crobiol,... picrobiol.?a parazitol*,. 1957, 8,, 2,. 191..
Investigations were carried out on a group of mice, in
whom motor-alimentary conditioned reflexes had been elabora-
ted., This group was subsequently vaccinated with the anti -
influ6nza vaccine, After vaccination#~ modification of the
conditioned reflex activity was observed, manifested by peri*__~.
pdi.t alternation of predominantly a Cation phases and pr-ea.
doninanti.y inhibition phases. No modifications appeared i* the
controls., These disturbances were slighter than those observed
in previous investigations, in which the animals had been ino-
culated with non-attenuated influenza virus.. It is concluded
that the law of intensity known in phydiology'is also appli-.
cable in immunology. The above mentioned disturbances appear
sooner in animals with a lively temperament, than in those,
with a phlegmatic character, in which they appear later.
386.I. MIRZA B . P..ATHANASIU-STROESCU t AL.PETREScU, AL. BRONITTI
a 8F.~ Stu of anti-influenza immunogenesis in white 'rats
*1tt -_varlo s
0-1 _. of - ziervou19 - 3
stem carried out.
1:utifri6ti6t i jbition test and, the-
m~ `hO Of nditi d refle es Stcerc. infra i
biol?Dmicrobiol... parazitolH., 1957;'8,:4.,587.
Rev..ScL Med. B 19598 4 , 61..
A study was carried out on thereactivity of an*U
in connection with the nervous system type., A number 0*awni-
malsq, in whom motor --alimentary conditioned reflexes ha been
elaborated, were inoculated with the anti-influenza vaceine
or with the influenza virus, Inoculation of the vaccine or
of the active virus causes an'accentuation of the inhibition
proces , more intense and persistant in the animals of phleg-
matic type, more discrete or of shorter duration in animals
of the choleric type.
After vaccination, hemagglutination-inhibiting antibo-
dies do not appear in animals of the phlegmatic type and are
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
236
to be..found in low titers . however,; in the animals of chole-
ric typez. "fter`noculaton withrf the 'active`-virus's the titer
is somewhat f -increased, but ithe s"ame ratio is maintained bet-
weep the choleric rand the phlegmatic} t' es.,
yP _ Booster doses',
erformed 11 =weeks later,;, wipe ,out these fferences between.
the two diff
t
eren
. types: of `the ne;rv:ous iysteino,
387. D.SARATEANU, B;*FUHRER. Study of anti=influenza immuni
ty in .p`revi Duals a iti-?vaccinYa immunized animalso
cerc.. inframicrob1.0 ' microbiol P.- 2o7... t ~ k 4 Report read at the Session of the I aatjtute
o ~ ani a obi olo ~ of the R.P.R. Aea .emy on August 117
BW ana117 . Kie amt L enio relations betweer, the influem,
r ai.x s i3olated im, the 1959 epidemic and the strains
i.m t6iO proooding yearoq It was possible to establish
Qte.3t to sx?e ip characteriaties were *aiatai ned
4~ o ar . tho vaxNiability that ouperwener with time.
zoo anal%L of the structure of t ,e Asians A2 stra.na ..
i 1951 and those isolated the 1959 epidemic made
.. ~ponolbl - to- we them a@ va oe1aation strains 0 since thoy exhii
a ou~~r a tiffesaie potentials
?._ appearax of xod A + A2 type of strains suggests
{'~.c e o~ to a
2zi mat e of both type@ of virus ? A and A29
"; :aC~ ~c~ t inn i oa leadi,AG to the forsatLe of newt a? of
AoM. Mt3'Xq Mo ON I0 ALo $'CUa ! J? ~ ;p! l_ 'tea 9 3c~
y~c n _f~ __ go V?,o M cripa 0 - Data a supra
n
p .6,nei 1i 1959h, T ado 1959a 82
influe sSa virus otr .ng isolated. in the 1959 opido-
, to the .utoe tomeus strains isolated in
>~~~~lq aye r~~hoaoc~u~q their antigenic structure
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
? 251 -
allowing their classification as strains type A/PR8, type
A2 and mixed strains A + A2.,
The variability^of.the strains isolated during these
years is shown and demonstrated by crossed hemagglutinati on-
inhibition tests.
The constant changes.in the titers of serum. hemagglu-_. .
tinoinhibiting antibodies are likewise shownsp a U+ aq;-?de-
Qj-z;;&;Cp anti-A2 HAI antibodies before the beginning of the 1959
epidemic.
427.- A.DEREVICI, AL,BRONITKI, AL,.PETRESCU acid C,2ATMARI
Preventive action of ' the Rumanian anti-winflueizza
va= Gripa-"-Date-aetlptA_epideffiiei-din, 1959,
EUMed; anw.1959; 161.
The prophylactic activity by means of anti-influenza
vaccination started in the R.P.R. in 1954, when experiments:
were carried out with the anti-influenza vaccine prepared
from mouse lung. The prophylactic section of the Institute
of Inframicrobiology subsequently prepared a purified ovo--
vaccine, with the aid of a special apparatus,. built for
closed circuit handling, which reduces the time of prepara-
tion of the vaccine, administered by intranasal route by
means of a sprayer with which the amount introduced can be
measured. Application on a wide scale of the vaccine in 1959
was preceded by various restricted vaccinations during 1956,
1957 and 1958.
It was observed that the vaccine prepared with strains
type A*PR8 likewise gave protection against the pandemic vi-
rus A2/1957. In the course of the 1959 epidemic, in a group
of 3,114 children and 1,907 adults the total average of the
protection index was 5*93 when the vaccine was prepared with
the same type A2 strain, isolated however in the 1957 epide-
mic- ?
The vaccine applied in the 1959 epidemic acted by in-
terference
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
i
?259?
4280- A?DEREVICI, N,DRAGANESCU. Cultivation of the influenza
virus in vitro in a fluid medium: The action of added
thymo and ribonucleic acidaComunicarile Acad.R.P.R.p1959e
Cultivation of the influenza virus in vitro in a69 e39.
fluid medium according to the Lee and Rivers method, was
performed with certain modifications consisting in the ad-
ding of thym, and ribonucleic acids.
After establishing the best concentration of embryo
tissue in Thyrode solution,. the effect of various nucleic
acid concentrations was studied.
A delay in growth of the virus was observed follo-
wing the additionoof thymonucleic acid and an inhibitory
action in the case of ribonucleic acid. These results are
attributed to the action on the embryo cells and not di-
rectly upon the viruses.
4290- AoDEREVICI, ALOPETRESCU, P.ATHANASIU. Experimental
sty z,.dy on _the..:Lmmunogenicity of the, inflaenza-viru.s'
strains isolated in the R.P.R, in the epidemic '.and
Postepidemic,e ,dS "."'Mierobiol:s pd!,- at It 61'e"11
epid.eniol. , - i959,.-48 4o5?409.
The imrpunogenetic relation between the Asiatic
t; e ': 2) of influenza virus strain, which caused
to i our country, and the influenza virus
type A/RRf ?i,~ o.Lat.od. in the postepidemic period in 1958 is
it N? C)
Tfhe r c f: ~> int; .u.n:,ity tests carried out on mice showed
protection, which does not correspond
o: some authors in connection with the
to man by vaccines prepared with
,: -'ainst infection with the A2 epidemic
The expe'o.!.v~.ts ca tried out on mice demonstrate the
advantage of *,-, Lactic acid evaporated 6o mi-
nutes before aerosol .spraying of the influenza virus as well
as after aerosol spraying had no virucide effect,
Still better results were obtained with fumigated fir-
vruL resin, (alcoholic extract of Pinus alba resin),,
?
Fumigation carried out both 30 minutes and 66 minutes
before aerosol spraying of the influenza, virus, led to survi-
v of loo % of the animals. The fluid alcoholic extract of
1inus albs resin did not have a virucide action.
b`5oe= G.MARINESCU . A simple procedure for investigating co1._,.
agglutinins: a " gt idin9" test- ii the dia nosis of iflf. a ii-
erobial primate atypical" pneumofiiaa Sts ? ces~co "'infraicrcm
biol0, microbiolo pa:ica ito1;; 1951, 2, 1-2, 1570
With a view to-investigating cold agglutinins for the
diagnosis of primary viral penumonia, a simple, economic r-ethod
is described which can be applied at all ages (especially
breast,-i,fed infants0
This method consists in drawing the blood into the
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
4w 272 ..
white blood call pipette up to the o.15 mark and diluting
it wha.tb. saline up to the -11 mark} the oentent is put into
hemolysis tube and then into the iie-box, it 6 be read
after 4..18 hours. The agglutination titer, when agglutina-_
i=m ocours, is approximately 1/125, the lowest limit at
which such a reaction can be considered positive for infra-
microbial atypical? pneumonia. If the reaction is negative,
can bit readily repeated, at momenta
particularly in the mouse,, certain of the aspects not-
in 3umah pathology.
severe (mortals
... Y ty rate 154 %) especially in .infant
inoculation to the common laboratory animals induced,
=he total . number' of pneumonia cases observed.,,, was excepti?-
Primary viral pneumonia,. representing about 10 % of
trim; 195, 2 ~2 ...
51:. LLVOICUU SCU, G. Ii CU. C;i 1 ?and:e.., er# ienta1
im~res ati ons o Vi al a c~ni 14,ren. Podia-
452,'_1 .VOICVLESCU, G, INESCU,. Pr mar$ Viral_..x~neum
in children., Clinic
,,__ ?~? ai, mor~ho~athologic and::exr~
r meht.al to o $to -dertd.. Pediatric ,. 19 ll`
Cl .~;al a n d i abser? at ion in hivzan
see Ire Pressed to ge t ier . %N t .. a comparative study on
:Laboratory animals in which some of the hi rtopathologic as..
ecte encountered in man were reproduced by inoculation of
pathogenic products. The clinical study of 26 cases of pri?-
-. ry atypical viral pnnumonia in children (6 months to 16
years), representing to % of the total number of cases of
pneumonia hospitalized, revealed the severity of this di-
sease in small infrn s in which the mortality rate attained
25 % due to nervous, complications,
The morphopathologic examinations showed at the level
of the' lung interstitial.infiltration with prevalent mono-
nualeara, vasoUlar changes that led to disturbances of res=
piration and degenerative.-proliferative leaioaa im the-
- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
ice, a u !. !r&SM p ", g i 7) r.Ie}! -virsl
L ,; brain) from 2 lethal r ..
xa,gs p:et`'v
severe route. of i
ffia ri o
} u;e-, on ,a0 showed that the most
respiratory routefl and the most viralen . ? _ ,,
II
matous degenerat?
broncho,vascular t xl o Discrete par xioh
system were obs?
Lions and modifications ~f the s e~enou m ? stems ~ ningo a
the new
In
ve in the other organso
The "?an.i.mai,G inoculate ~ml,
ijnfiltrations were rt~tc do ,- tho eni . pro tof
273?
? hR! lul: gs and spy e A0
affected organs..?were:, the i s
ere .Y
The most sev
mesodermal componen of the nervous y stem and
t
Gib ?? ? ar? _b d newo
-
rec the live an,,
l.e er d"Ag (I ki
3'all A Ti r l Es E o 'A y MoM.IRSA I9 SoGIR ARJs.
d gy a T l CU R S `,fi9 ?o ;E3A~tASIST.a
L'1~.Ab
the CtR ~ ; ' aw e S p u
oft t- .,, 9 R~ 6" if?fT,t7 f d opid ffii o B liar St 4 5
19 8
S ^ases of a,o?,te peaania we adm
7L,(, ~ ?' t. o
lesionat the ba
pneumonia lesicrss o eri.co
wpF~al r mixed
were constantly ne~sQ
g nth n?eoropti oases
t: , , The a i logi? tud 's on the other hands revealed Lin
,ra ^= lases a non-hema2utinatzag inf ramirob3:a1 afagaui;
path g 6 n i ~ . for mioee These animals died i &5 days
or days after peritoneal
ino ^ .a i.on by intranasal route or A
t
ino: ulatio -:o The virus passes L'"~Ugh the Seitz E,93 filters
ra maintaineu~ in saline at 2o~'Q r i glycerin ~t x'40
in neutralized b the arati~influea A8 9 A20 BO C and
~^a and. dove not pros immunize with afty of the influe ~
Thy histDlogi,,e lesions were eithea; p-.
in 1 ~L
sad t the .5, oond -Clinic of pediatrics, Be har?sts of Which
2 wer r diagnose ~ as atypical pneumonia o three of thec
tomatolo=
eases alone. s u pi? eaio In view of the clinical syp
" hi.st .1ogi@ lesions * and serologic reactions, all the ca-
erp listed in the group of primary atypical p ,eumoni.a
taJYe~ ) and influenza atypical pneumonia (1 ease) ?
In. 2/5 of the cases the evolution is supraaaOut f 01-
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
274 As T
za virus,-)s isolated in our country, pathogenic for mice.
It is not inactivated by penicillin, streptomycin and ter.
ramyci.n.
454?_ V.PANCU, AL.,PETRESCU, P.ATHANASIU, Considerations on
a viral respirator epidemic in the s rin -of 1 8-s
in the town and district of Boto ani o-'Symposiur-on
the _' 1957?1958 _ ifAflu?S2a - epide io?"Bacharest, December
4-5 1958.
In the March-June 1958 interval, 367 cases of acute
r~ -piratory affections were reported among all infants,
taking on the aspect of an epidemic outbreak. Of these ca-
ses, 166 were diagnosed clinically and radiologically as
.ypical broncho:+pneumoniat with a severe evolution and a
:or cent mortality rate. The onset was usually insidious,
niter 3-4 days the symptoms became more se1rnre and the ge-
noral state developed towards collapse accompanied by the
other characteristic e'_gns of broncho-pneumonia.
The attem S ; made at isolating the causal agent
from the naso--pharyngeal secretion or the .-Fziezes revealed
in 2 cases a non-hemagglutinating virus, pathogenic for the
white mouse by intraasal or intraperitoneal inoculation.
The animals died 14-15 days after intranasal inoculation
,id 4-5 days after intraperitoneal inoculation. The virus
passes through the Seitz EK.3 filter, and is stored in
-,.yeerin at + 4?C or in saline at - 2ooC. It is not neutra-
lized by anti-influenza sera type A, Al, A29 B, 0 and D.
w' -d does not cross-immunize with any of the other influer -
za viruses isolated in our countrye
The virus is pantropic and can be revealed in all the
organs of animals which died of the experimental disease.
It is not inactivated by antibiotics, penicillin, strepto-
mycin and terramycin.
FOP OFFICIAL USE O
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
- 275 -
455.- D.;;ARATEANtN.DRAGAN'"('TTY EL.NASTAC, EL.OPRESCU,
in -bulmonary_
s:^Conferenc~ f_th.e-CzecYiosib~'a8fan
viral affection
virologists, Smolnice, 14-17 October 1958.
A serologic study was carried out in 130 cases of
primary atypical pneumonia.
The results obtained show that influenza viruses
of viral
type A and A' are incriminated in the etiology
neumonias in 42*3 % of the cases, the ornithosis virus in
p
36,9 %, nea-rickettsia in 6?1 %, primary atypical pneumo-
nia virus in 2.3 io and iymphocyte choriomeningitis in o-.76 %
of the cases.
Attention is drawn to participation of nee-rickettsi-
as in the etiology of atypical pneumoniasi and stress is
tests
laid on the importance of double and triple serum q
in order to be able to make the serologic diagnosis*
4569- D.SARATEANU, N,DRAGANESCU? Non-influenza viral
InO & ,; AAm3 n-Re-Dort
neumvula~~~~_. - - ~0ember 195
read at the , Inf liUebta - Sy~po sit ., `4" 5-Bucharest.
Experimental and serologic. irestigations on the
are described.
problem of the etiology of viral
At the same time, the data in literature, refering to the
etiology of viral pneumonias are reviewed.
From the data presented, the possibility of esta-
blishing the etiologic diagnosis by means of serologic
reactions, isolation and identification of the virus re--
sults?
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
Mixoviruses 276 -
4 D.SARATEANU,. AL.BRONITKI, Laboratory diagnosis of in-
fluenza . Microbiol. , ' parazitol. , .. epidemic ?_P 1959i-
3.251*
The paper is a synthesis of the diagnostic methods-ap-
plied in influenza. The dynamics of the data are shown and
mention is made of the products to be collected from patients
and neoroptic cases, of how to prepare the products for ino-
culation, inoculation of sensitive animals, embryonated chick
eggs and tissue cultures. The use of the serologic (comple-
ment fixation, hemagglutintLtion and hemagglutination- nh3bi-
tion), cytologic (Koleaditzkaia and Panthier) and histologic
methods of diagnosis are. described.
4:8.- R.PORTOCALA, V.BOERU, I.&AMUEL. The...role of..ribonu -
cleic' acid in the infectivity' of O.Sfiflueh ,a'yii^us.
St.cerc.inf'ramicrobIol; i ,1961! ?l -1-#%i;...,....,-.,,...M.,
The phenol extract obtained from the influenza virus
contains ribonucleic acid that is the infectious factor of
the virus since, inocu-lated into the allantoic cavity of the
embryonated egg it induces the sensiti~vf, cells to syntheti-
ze the 'homologous virus with all its morphologic, antigenic
and infectious properties.
The ribonucleic molecules are very labile and under
t action of ribonuclease, temperature and prolonged storm
.e, completely lose their activity.
The new influenza virus strains produced by intracel-
Wlar synthesis under the action of ribonucleic acid, pre-
sent certain biologic characters that differentiate them
from the initial strains.
The virus strains of antigenic A2 type never release
active' ribonucleic acid, probably due to a high proportion
of incomplete elementary bodies. -
Recently isolated type A virus strains appear to re-
lease a much more active ribonucleic acid them the strains
carried through numerous passages. in the embryonated egg.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
277 ?
Synthesis of a virus prom its ribonucleic acid may be
obtained not only with simple nucleoprotein viruses, but also
with more complex viruses, such as influenza virus.
459.0? AroDEREVICI, AL?BRONITKIe Study of tissular antiinflu.er.-
za antibodies iu animals app' ently?resistant~tO tbe_~
experimental infedtio.L,, cer inframieroBio ;; -fi%6o9
.11
1
During.investigations on the immunogenesis in influenza
tissuiar..and.serum antibodies were investigated in animals
apparently resistant _...:-uenza (rats and rabbits), subjec-
ted to repeated bleeding and perfusions. Separation-of tissu-
lar from serum antibodies is best obtained by the technique
proposed. by the authors, i.e. collection of organ fragments
before and sifter perfusion. The serum antibody titers warn
higher than tissular titers. ' Tissular antibody titers, before
and after bleeding, present differences which are more accent -:
tuated in the spleen and liver, organs with an ample circu-
la.tion,e The highest tissular antibody titers were found
the lung' ' a fact explained by the pneumotropism of the virus;
then the kidney, through which the virus is eliminated. The ,.
liver and spleen present low titers.
4- P,ATHANASIU
460
AL.?PETRESCtJ. The effect of cultivation of
,
,
the' influenza
irus* straif-A1' /53+1 ^ahimafis"'~v3tfi"ID~O
dified readt
y;- St e~daiSff~midFSbi01 I9 O 1-
ll7.--WT,ifobt,;Bled. Roumm 1960, 12.
An influenza virus strain adopted to the chick embryo
and slightly pathogenic for the white mouse was cultivated in
normal mice and in mice previously infected with 1/2 LD50of
street rabies, herpes or poliomyelitis viruses, or immaftized
against rabies or poliomyelitis.
The variants obtained were. studied as regards pathogeni-
city,, hemagglutination, antigenicity and immunogenicity. The
values of the biologic properties of the variant cultivated
in the rabid mice were close to those of the initial strain
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
- 278 -
with an increase of pathogenicity and antigenicity. The
biologic properties of the variants cultivated in the ani.-
mals previously infected with herpes or poliomyelitis, or
immunized against rabies or poliomyelitis, showed a decrea-
se of the values as compared to the initial strains, except
for the immunogenic potential which was higher. With the
virus cultivated in normal.mice, the pathogenic potential,
hemagglutination and antigenicity fell to zero, and only
the immunogenic potential was maintained, at a lower level.
461.- A..DEREVICI, AL.BRONITKI, AL.PETRESCUQ Biologic aspect
of-influenza virus strains. isolated in-the Raman1ah-
Pe6fi1a's_Rep blie, Relationahi$,t the Pebraar~ -""
MBtch'epidaffiid-sttaies;-St: eP ;-inrmm1erobial.
A comparison study was carried out on the influenza
virus strains isolated in the country from 1953 to 1959;
showing the variability of the autochtonous strains and`
the appearance of new types (A2) .
Study of the influenza -vi us strains isolated in
the country during the 1959 February epidemic showed a lack
of homogenicity; during the first days an :a~uf]:ueiza.lstrain
t3 p; A (PR 8) was isolated, then type -122+4,4 finallyA+A2.
The strains were isolated during-the-epidemic period
in various university centers in the country.
Y.COPELOVICI, M.GRUTA. Experimental investigations
on influenza virus - straing-igolate -drLtSng"t$?""^^
1959 _ gebr~ cry-M~trc$~eldemio:St oeo i!remierobioi.
Study of the pathogenicity for mice of some influen-
za A2 and mixed A2 + A strains, isolated during the 1959
February-March epidemic, showed a low pathogenicity for
this animal. The mixed virus strains can be adapted to
mice after previous X-ray irradiation of the animals
(45o r).
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
- 279
463.- E.SORU, A.DER1CI, AL.BRONITKI. Chromatographic
determinations of ' ce- -- ~;. amifoacids: frog influenza
virus ains isolated " in the Rumanian People's Repu-
bl during the 1959 e ?idemic Sts care e itf r idtobi(l.
196o, 11, ... 3 ..357 ? _ _ ..
Chromatographic analysis of certain inoacids from
influenza viruses isolated during the 195$ a uid:eme (Al, A2
and mixed A+A2 strains) and a physical mixture in egt.al parts
of A and A2''eluates, showed:
1. More significant gt.antitative differences as regards
tirosine', glutamio acid, argiuine and the aspartic, serino
and glycocol grc,s.p; iidl imal differences were found for leu-
cine, alanine,.phenyl.-ramylalanine and no differences for
valine and lysine.
2. Comparison of aminoacid concentrations in t the mi-
xed strain and the physical mixture of the component A/59
A2/59 strains, showed equal values in 66 % of the aminoacids
determined.
3. Comparison of aminoacid concentrations in strains
A2/59 .and strain A2 isolated during the 1957 epidemic, sho-
wed different values for glutamic acid and lysine and small
r1 "fferences as regards the concentration of the other
ac ids.
These results characterize the correlation between the
chemical substrate and biologic properties of the various
strains investigated.
464.- A.DEREVICII L.PBEDESCU, AL.BROITITKI, I.COTARGEA?
1'articul itie f '_.. fl x aza. ti.'..:. strain of. the
t Sate - t . . r -1959 .
hiic dur
mitred A+A 1 poi eted" fa" Re--
S cerc. ramier-a'bi o1-ar I96o, -11w ;3~r 375.
Vi.~ ologic study of strain Pa/ '; iso1et+ towards the
end of 1959, showed in inoculations to mice predominant neu-
rotropism. Intracerebral inoculations permitted.iieolation of
the virus from the first passages.
Adaptation of the strain to the ' lung is obtained only
with products from passages in the brain or the allantoic
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
28o
fluid of embryonated eggs, likewise after the first passa-
ges. These characters differ from those of the mixed A+A2
straiiz isolated at the end of the 1959 epidemic, -probably
due to a new constitutional structure of the virus, which
underwent, during the 7 months interval,, numerous passages
through the human organism*
The hyperergic character of the;d.cliAie 1,.&volutioa
in children may be=attributed to the bi-ologic characteris-
ties .of virus Pa/59, as , well. as to the_ specific reactivity
infants,
,- A.DEREVICI. Heterogeneity of certain influenza vixus
variants tested _.b_ th? - techni .. to of end infectious
dilutions: F,t:oerc; i8fi's i robiol:, ..196o ;ii;4;55%
Investigations were carried out by the technique of
;nd infectious dilutions on some influenza virus variants
isolated during the 1959 epidemic, presenting from the se-
rologic viewpoint the com.:lex antigenic structure of type
AandA2.'
.Determinations were. done with 1,75 % and.33 % of
ID50 and seroneuitralization with immune serum A and A2.
The strains obtained, were..1 hibited by both sera A and A2
&n d others only. by~ A2o t . of_ type A2, resulting
from inoculation.of 3 % of ID50, Neutralized by antiserum
A J o f , strains A2 and A+A2.The
,perimezrtel ;results : showed that the strain investigated
1 a transition strain, with an instable structure, that
Y be dissociated--.in the course ' of the passageso
'I).MIF1ROIU, P.ATHANASIU,. Y.STRULOVICI M.POPA. The varied,
etiology of certain influenza-like cases with an epidemic
character -Report--read_at-the"session'of the-Institute"'-
of Infraticrobiology, July-4. 1960.
In order to establish the etiology of certain influen-
*.A1ke cases with an epidemic character,.investigations were
carried out on 25 of'17o cases diagnosed clinically as in-
fluenza or influenza with pneumonia.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
281
Cytopathic agents were isolated in tissue cultures
from 8 cases and identified a:~ i orming r art of the APC group
by complement fixation.
A virus, isolated in mice, was neutralized by conva-
lescent serum and Boto-antiserum. Attempts at isolation of
the influenza virus remained negative.
The predominant adenoviral etiology of these cases
became apparent and attention is drawn to the existence of
epidemic cases caused by APC viruses and the necessity of
bearing in mind this possibility in future etiologic inves-
tigations
457 $.PCRTOCALA, S.DUMITRESCU9 N.IONESCU, I.SAMUEL9
V,BOERU. Replication of the influenza virus y mean
ICI Morpholo$id as eat
of viral ri.b?nuclei acid, .--
ofthe generated ~rizsusm"Courtin: Aead.R.P.R. 196O;
A study was carried out in the electron microscope
on the morphology of the influenza virus generated by the
ribonucleic acid extracted from a strain of the pure A an-
tigenic type. The morphologic aspect and the size of the
viral elementary bodies obtained in this way do not differ
as a rule from those of the initial strain. Thus,, the mean
size of the Virus from the first culture and' from the first
passage in embryonated eggs is leoW7 ? o.7 m1 ? This is
identical to the size of the initial virus,, .from" which the
ribonucleic acid was extracted.
Serologic investigations demonstrate the antigenic
identity of the ribonucleic acid generated strain and the
initial strain. The morphologic results obtained eonflxm
the hypothe sis?'a(ftording to which the receptive dell syn-
thetizes the @"V'Iete influenza virus starting from a tem-
plate of the nucleic acid molecules introduced into the
cello
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
2.82
468.- R.PORTOCALA, S.DUMITRESCU, N.I.IONESCU, AL..B.RONITXI
Morphologic aspects of an influenza virus strain of
the"mixed. A + AP " type as compared to types A and A2.
.......
Rev. Sci. Med.196O,,2' 3-a-49.2230
In view of the experimental results obtained, the authors
consider the mixed influenza virus strain as a physical mix-
ture of two different populations, each belonging to a diffe-
rent determinant type of influenza virus.
469.- R.PORTOCALA-'., V.BOERU, I.SAMUEL. Replication of the in-
fluenza virus ,y means' of viral rib6 ucleie' acid;"'"'
IV.- Study of . rib0Yitaeieaee 3.i"e~~ated ';agga` of va-
~iou.s
Study of ribonuclease in chick embryonated eggs showed
that in the chorio-allantoic membrane and the allantoic fluid,
the enzymatic amounts follow an ascending evolutions. in terms
of the development of the embryo.
It is recommended to use, for investigations on the in-
fectivity of influenza ribonucleic acid,, lo to 11 days old
eggs, since they contain the lowest amount of RN-ase.
The likelihood of certain quantitative and qualitative
changes of RNA in terms of the season in which the virus is
cultivated in eggs and, implicitly, of nucleic acid extrac-
tion, should be taken into consideration. These changes
might explain, together with the evolution'of RN-ase, seaso-
nal failure to produce the influenza virus from-its RNA.
470.- R.PORTOCALA, M.ANDREESCU . Replication of the influen-
za virus by meafig"of viral ribonadleie" 46id.:V . IYifluen-
__ rr r
ce of the giial.ilF,ar~ of phenol on_ribonucl.ege acid"acts=
~I Awn ~~
vity. St cerc "infrsmicrobiolo, 196l; I;-77;- _
The impurities found in the four types of phenol sup-
plied from different sources and used for the extraction of
viral RNA, were identified and determined. The presence of
heavy metal ions, especially lead, in some phenols, accounts
for the inactive RNA obtained.The smallest amounts of impu-
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
riti es are to be found in Soviet phenols with which an active
RNA is constantly extracted.
It is recommended, in order to prepare antiviral vacci-
nes attenuated by phenolo to use a pure solution of this subs-
tance so as to avoid total inactivation of the vaccine virus
471?. O.RURDUCEA0 MGCEPLEANU0 R.CAPRARUe- RKOlution_of ^HAl
antibodies in white rats immunized a : aifi.-Winflflan.2as
u.ndor the influence of "' l and- __ i..t ?adiatiOnB; " St.
cer{ infraini.erbbio1 ... ,96i,~ "~ is 1370
Investigations were carried out on the evolution of HAI
antibodies at 4. 8fl 12 and 2o day intervalso following ino=-
culation to white rats immunized against influenza, 25 )C
amounts of 1131 or P32 or stable iodine or phosphorus. at a
pH and concentration equal to those of the radioactive solu-
tions.
It was found that under the action of gamma radiations
emitted by I1310 the titer of antiinfluenza HAI antibodies
significantly decrcasess as compared to the control group,
P32 stable I and P do not influence the titer and, eve-
0
lution of antiinfluenza HAI antibodies.
4720? PoATHANASIU9 M.POPA? A study of hema_g l utino.i,ibiting
influenza' antibodies ifk-rabbits' ihoc .latefl"wilt
a"virus into the Cori ncti rai sack"afterlig~t, on-and
sed-tion of"the" lachr maT cans:Co "St: "cerceinfrafnicr~b ?
19619.x,29 229m_"-.r--'- -- -_
Inoculation of influenza virus into the conjunctival
sack of rabbits whose lachrymal canal had been ligated and
sectioned, resulted in a moderate increase of influenza an-
tibodies in the blood serum- This result points to the poa
sible occurre e6~7 f influenza' infection by the conj tival
route, i.e0 iA&SDVe ado -tly of the nasopharyngeal route, and
confirms similar investigations reported by Cl.Hannoun.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
284
473.- ACAD.ST.S.NICOL U,. N.DRAGANESCU, V.BOERU, N.I.IONESCU_.
Saectrophotometric investigations in UV light on_ribo-?
nucleic acid extracted from the influenza virus inacti-
vated by copper ions, St.cerc.inframicrobiol. 1961,126
3, 285; C.R.Acad.Sci.1961, 213, 1503-
S I tudy of the absorption spectrum in UV light of tibo?
nucleic acid extracted from the influenza virus inactivated..
with copper nitrate in. a 0.5% concentration did-not show any
change in the ribonucleic acid curve.
474.? R.PORTOCALA, V.BOERU , S.$4RAGEA, IGSAMUEL. Reproduction
of influenza virus with the aid of viral ribonucleic
acid. St.cercfi framicrobiol. 19618 12 , 3, 303.
The ether-phenoi&... axtract of influenza ribonucleic
acid, subjected to dialysi? precipitation with alcohol and
redisolved in normal saline, presents in ultraviolet light
and. absorption curie characteristic of. ribonucleic acid, with
a peak at 258 Up.
The absence of proteins in the studied extracts proves.
once more that the infectivity of these extracts is due sole-
ly to ribonucleic acid.
475,-R.PORTOCAI,A, N.IsIONESCU,. S,DJ'MITRESCU, V.BOERIT, I.SA-
MUEL, E,PACURARU.ReproductioU of the influenza virus
with the aid of viral ribonuc,leis acid. VII.- Morpholo-
gic aspect of ribonucleic acid, St, cerce inframicrobiol.
1961, 12, 3, 3o9?
A study was carried out in the electron microscope on
the morphologic aspect of infective ribonucleic acid extrac-
ted from influenza virus. For this study the preparations
were made with the products collected begore the end of the
extraction.
The electronooptic examination showed the presence of
characteristic strands, In most cases these formations are
joined together along their lenOforming thick clusters.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
285
The diameter of the finest visible strand in the prepares-
tions is 30 . . Certain hypotheses are emitted on the.-
imp-possibility of evidencing finer strands. In the products
collected at the end of extracton the strands described
are no longer found, a fact discussed by the authors.,
On the same preparations, the presence of viral ele-
mentary bodies, with a morphology deeply altered by the..
btutal action of phenol, may likewise be observed. Their
presence may be accounted for by the fact that the ribonu-
cleic acid was obtained from incomplete extractions,
476,? A.DEREVICI, G?BAI US, AL.BRONITSI, G.ISAIA._ Local-and
Eeneral paraallergic phenomena obtained in the rabbit
with .the influenza virus. .St, , ccre.. infremicrobiol.
19618 2n 3, 335; Rev.Sci.Med. 19619 3-4.
The findings of the authors show that the influenza.
virus may produce in the rabbit localized (Schwartzman)...or`
generalized (Sanarelli-Schwartzman) pars-allergic phenomena.
Leuko=and plateletpenia become evident 2 and 4 hours after
the inducing injection, followed by hyperleukoeytosis and
return to normal of the platelets. Quantitative differences
are obse:rve.d...in..the.._.contrals...._..._
The same hematologic characteristics were found. in the
group subjected to the phenomenon of generalized para.-
aller-gy, differing however in that leukopenia appeared only after
72 hours. The controls of this group presented marked quan-
titative differences, which makes it possible to attribute.
to the influenza virus exacerbation of the phenomena obser-
ved?
Pathologic histology of the kidney and lung of the..
rabbits subjected to the Sanarelli--Schwartzman phenomenon_
showed the intervention, of hemorrhages, thrombosis# lympho-
monocytic infiltrations, blood extravasations, far more_ac-_
centuated in the rabbit sacrificed 4 days after the inducing
injection than in the rabbit sacrificed after 41/2 hours,-..
The aspects observed in the lung and kidney were not obser-
ved in the liver and brain.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
286
477OQ Al0BRONITKI9 AeDEREVICIV G.BALMUS9 PHAM SONG C otra?
checornm in --i--
uui? k,ne action of histamin. St,cerc,4inframicrobiol.
19619 122 3, 367?
Histamin administered in the form of aerosols to mice
subjected to influenza infection brings about quantitative
histopathologic changes in the tracheal cells, studied by
means of the Panthier technique..
- .. The tracheocytogram of mice receiving,,) besides the
virus, histamin and an antihistaminic (suprastin), takes,
on an aspect closer to that observed in the mice that recei-
ved only the virus.
The control mice., receiving only histamin or tracheal
washings with normal saline, did not present giant cells
with a vacuolar contents nor catoplasmatic formations con-
sidered by some authors as virus colonies4
Administration of histamin to mice inoculated with ma
terial from an influenzasuspect case,. may help to reveal.
the presence of the latter by means of the cytotracheogram
study, according to the technique described in the paper,
478.c A?DEREVICI. Investi ations in the problem of influen4
za in the Rumanian People?s Republic bet.
weep 195o and 196o*?Stecere9inframicrobiol.
1961, 12, suppl., 57?
..........
_ . _ The main stages-?in the study of influenza during the
9 1950 1960 period are described
The.results of isolation and typing of influenza virus
strains in Rumania are reported, as well as comparative stu-
dies on the various strains corresponding to the different
epidemic_periods6. The author likewise describes the first
antiluenza vaccine made in the country and the results
of its mass application.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
0287 .a.
479 A.DEREVICI, AL.BRONITSI, GoBALMUS. The allergic
factor in experimental influenza infection. The role
of histamin. St.,cereo inframicrobiol., 1951, Ijb
supple0 235; ReveSoi.Med. 1961, 6, 1a2, 33.
'The allergic factor in influenza infection was inves.
tigated by exposing mice to histamin.aerosols following
their infection with influenza virus. The disease was
found to be exacnrbated,it was accelerated and the mortar
lity rate was higher. This effect is definitely attenuated
by the administration of an antihistaminic (suprastin)...._._..
Substitution of the antihistaminic by chlorpromazine
(Largaotyl) did not result in an improvement of the diseaa.
se, as expected, but on the contrary, in enhancement of in-
fluenza infection.
Virus rnu' tip]. i cation, as tested by hemagglutination
in the lungs of the dead mice, is not influenced by adminis :
tration of the above drugs0 This points to he presence of.
neuro=hormonal disorders in the course of the influenza in-
fection processo
From those re .lt4 3 9 the existence of an allergic i'a~-
for in the course of infuenza infection may be inferrede
48o AL.PETRESCU, PATH: NASIU0 Alte?ations of the tophofl
dria, in white mice during influenza immunization0 St?
Gem, inframicrobiolo, 19610 12,9 supple, 249e__
The authors have studied cellular mitochondria in
white mice undergoing influenza immunization, _ as compared
to the controlsa-._.
_.v It -was found. that between the 5th and - the loth day-
following immuniZati-one.no mitochondria-were demonstrable
in-the tracheobronchic epithelium, liver. and skeletal mus
6~.e 0 This phenomenons _vhich is reversib,ie t is assumed to be
due to changes in their biochemical c onstitutione
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
288
gjUI.VOICUI2SCU G.MARINESCU. Nervous involvement ill inf luny
The third Congress of the Czechoslovakian microbiologists
on respiratory viral disease* # Ma A G1.
During and after the influenza ppa emic due to virus 'x20
38 cases with varied nervous ' "a-mI t were obeerveds 13
cases of serous meningitis, 7 encephalitis, 5 meningopncepha-
litis, 12 facial paralysis and one transverse myelitis.
. The etiologic diagnosis was confirmed by the presence.
of inhibiting and hemagglutina.ting antibodies, studied dyna-
mically by the absence of enteral viruses `in the cases with,
facial paralysis) and on the basis of epidemiologiio finding; "4
The neurologic signs disappeared without sequellae in 2
to-12 weeks, except the case of transverse myelitis, in which
a syndrome of motor spasticit; persisted.
4820= Y.OOPEWVICI, MLGRUTAO .BQSGA.T, n s ations on_, tb
mumps vaoaine repared from au tochto ous. stra .nsv+8t
sere. inframicrobiolo, 1961, , guppl?, 2070
_ A.. live ovovaccine against epidemic parotiditis has been
prepared from three autoohtonous mumps virus strains after
26=31 passages on embryonated chick eggse
Two types of vaccine were inveatigatedx a dialysed.vac,
tine according to the method of A?A9Smoroddntsev and an undia~
lysed vaccine. As compared to the dialysed vaccine. the undia-
lysed vaccine was found to have a higher imfectivity as well
as a higher antigenic potential, as determined, by hema,ggluti-
nation-inhibition antibody titration0
owing to its qualitieso as well as to `;.S simpler pre-
paration, the undialysed vaccine is recommended for use in
current practice
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
m 289 4
AOPQC0 483p?
I ?ADERCA9 MGIANCC ACUe . GODANIELSCU. Investigations on. adeennovia' ._
.aq_--n lo- Isolation of adenovirus strains
I _:i
in the-Rumanian Pe ople ft s Republica St0cerc ,infra
mic robin l o ft l96o a ll., 2 D 2290
The authors report the isolation of 4 adenovirus .
strains on. human embryo cell cultures obtained by tryp.
,1nization0 Two strains were isolated from the nasopha-._
ry ageal washings of patient& with respiratory affections
and two strains from the feces of patients suspect of a
Coxsackie infection and presenting moreover a rhinophan
gea;1 infection, The etiologic diagnosis was based upon ....
complemeEE fixation and characteristic alteration of the
human c. ,bryo cell nuclei in OQ in vitro ?D cultures,
6-4,Q M 0I OO SCU9 IOADERCAp Inves? do on adenova-
ruse ll, Gel lesions prod,uce l b denoviruse .
-4embryo cell ultureso a tocerc.oio-nframicrobjola
196cq llv 2v 237,
The characters of the cytopathic effect produced .... .
by adenoviruses in human embryo cell cultures were studied:,
The lesions oboerrved. were similar to those described in
other typAR of cells. The characteristic aspect of cell
lesions produced by adenoviruses render them easy to
identify..
485,= AJ REVICI, M0ZSTRATIa Onmparative investigations on
the diagnosis of adenoviral diseases m
complement fixation and-M
r el prop i itat$,on.0
St,ocerc0inframicrobiolt,p 19619 12? lD 630,
Comparative investigation, for the diagnosis of ade-
noviral, diseases, by means of complement fixation and agar
gel precipitation of 243 sera from ?Ed.vi.duals with respioo
ratory manifestations in the acute Stage or eonvalescone
as well as from apparently healthy persons o showed a pro-.
portion of 92085 per cent agar gel precipitation reactions
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
in the group of sera with the highest complement fixation
titers (1/128) and a proportion of 13e,33 percent in the
group with a complement fixation titer of
The existence of a number of negative 'complement fi?.
Nation testso but with a 7.8o per cent of positive precipi-
tation reactionsg would suggest that the latter test might
give earlier information on the etiology of adenoviral in.
fections9 a fact that Mill remains to be proved by further
dynamic investigations.
Stocer o
4860- L.PREDESCUo P~qgress in adenoviral.diseaseso
inframicrobiolo fl 1961 e 12 , 2n. 2330
A brief history of . the data in literature concerning
adenoviroses is drawn up? listing these agents in the group
of respiratory viruseso , according to the classification of
Gorbunova and the W.H?O. The clinics and diagnosis of adeno-
viral affections are then reviewedo
The properties of the viruses are dealt with in detail.
New data are.cited from literature regarding% the multipli-
cation cycles of adenovirusesfl production of characteristic
cytopathic effect after inoculation into tissue cultures of.
deoxyribonucleic acid extracted from adenovirusess the onco-
lytic effect of adenovirusesa their antigenic component and.
hemagglutinating properties; cultivation of adenoviruses in
tissue cultures'e
Data are likewise presented on vaccination in adenovi'
ral disease@8 suggesting vaccination of deoxyribonucleic acid
extracted from the virus 9 according to '? r` owski who rocom-
mends vaccination with pure RNA in other viral disease!,,
487,x- A GADsStc $,.NICOLAU a S.DUMITRESCU 19 N.?I ?IONESCU, UA.id Ti.
AN e I.PACURARU Morpholog%an es in human_amnio=
tic cells fected with adenovirus type Stpcerco.in?
framicrobiol?? 19619 12.L 3a 289; Rev?Sci.Medo196196 9
3.4A kinetic study was carried out in the electron micros-
cope on the morphologic changes produced by multiplication of
adenovirus type 3 in human amniotic cell cultures,O cellular
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
2.91 -
line F J4,0,
These changes observed for the first time by the authors
are present especially in the nuol.t? They are characterized.
by the presence of complete.$ polygonal elementary bodies., dis.
tr .b?}ted throughou tl.e nucleus and, protein crystslso, conside-
red by the authors incomplete forms of adenov rus.O
X88-
L o L~ R CI 0 r D SGU Z .ISA.IA trjo ' s t3 ov .
0 an ibor es i various a e~ a ou, r amola
t ~. ~opt~la on, of the l B-a~, i ..Peo le_a s Republic ? t
-.r
__ - - te
cerc, inframiarobjola fl 1964. 12p. 3o 325w
. . Investigations were carried out on antiadenoviral comple-
r,en fi,ng antibodies in 699 ser^aj, considering as positive _ .
tom, seaoa with titers of 1/8 and higher? The proportion of po-
sl,tive saga0 classed according to age-groups was 29. per cent
the 1?12 months age-group, 67 per cent in the 1-7 years
a s.g.-ecup, 84 per cent in the 7-18 years age-group, 73 per-..-
cent in the 1825 years group and 58 per cent in persons over
25 years old.
The lowest proportion is therefore to be foal, in the,.
J-12 months age-group (29 per cent)0 In the other age-groups
the proportions.are about equal, the highest values being i .9 u
fo n(' in the 7 - 18 years age-group (84 per cent) and in re-. .
tits (8o,.2 per cent) fl a fact Which may be attributed to clo.
~~. contact under the living conditions of these rou
g ps'o__
The number of isolations of adenovirus strains which, was
smaller than the proportion of positive complement fixing
reactions, points.to the utility of this test for a retros-
opeative diagnosiso .
i,89 e- G Q f NESCU O . D JA g GeISAIA0 L. PREBESCU J: Y?CO PELOVIC I .
LOIOBANESCuo Amite obstructive laryngitis in the tour-
se7, of infections due to viruses from. the APO Zrou ?
St? cerce $ inframicrobiolee 1961., l2 fl 3?~.y~7
Five acute obstructive cases of laryngitis are reported
out of 12 laryngitis cases caused by infection with viruses,
of the APC group:,, There was a definite croup sy ptomatology
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
in all the children under the age of 2.. in one child aged
5g who had croup for the third time o and a milder fora in
an older child? The diagnosis of APO infection was established by
isolation of a cytopa'Gfl genic agent belonging to the ad, -.
novirus group and by kinetic study of complement fixatio,e
49o e A?D VICI 9 AL?BRONIT 0 LoPREDESCUm lnve sti at ::.s
on the etioJ~ogyof soae focal respiratorvir .l d, r
es in different cen s in t h'2 count duri t ho first half 2j _136o# Microbiol?g raraZitol,e g epidemi ':= ,,:,
19619 6g 499e.
The authors show the result of investigations carri ~F?~ `k r~ tr. SA ~ > i`?t` C` .. ~^. -.,... w r . *^~*~.*.. ,..:...".~.s:.aeu ak ap:t a..w
~` ve para11e1~ exper meat s . ca ri,ed o oa r58 piglets
showed that. adsorption on aluminium hydrox ie.;doee note n-
? fluena.e theF~ d f~ferenceLoe that exist between the immunogenic
k iJ . ~~.w . oFM?~evt ~~
value:Rof:~virul~en ex~traaz ~ of liver-or bra no a A a ?.! V wi#i1 i+T. Y' t Y.1i .11tY '. ~.2 iL:1
Af~te:u adsorptionjjon;aluminium hydroxide gel,T the vac
~ ? ~. z ~~~.~ x~ .~. awn a~ ~. e-~
passd fromlaad,,:brain {'have a'' ~:~
tines. re. ' r
tt.lower 4mm6pogenia
~ ~
~ T.1 `~4?.:.'.~ 3 M ~Vl fie.. +T y i ~.
value .-than he.~ adsorbed, extracs of formolartR ii spleen.
v, t R l3
BAg;.compareed,,t Cthe immunogenic value of .the. yadsorbed
.} a ati ' . /e+.:Y 1
splenic~. extract,,. the. ,value , of the adsorbed he{patic extract {
repro-senta>lapprcLx mately`5o bper, eent.~~andt,adsorbed brain es-l
tract on4lty about? 20;oper Beat lof,rythe Amkunizing.,,value of the
adsorbed'e err
521:-{ VI-sWYNC'RR'ADN Kc L. ~; I.I GRIN Y, I.GI QRGRIU, N?BIRBU.
a dteric1dal-_action_of. tri
paf1 the .an d' acr flAv'lne
to asteurella ink;the' bls .d ue~l.d~foP.~h rperim izar- j
0ption*1'6fLranCAJAL. Laboratory dimosis of some ,yagayellas
y
(benign lym ranulomatos s.. trachoma and-viral, prima
atypical eumohia) b y " ieaihs of the WeilmFelix tes e
S t - e - mic-ro' Viol"
1951. 22,, 1-49 3510`
Based on the presence of certain antigenic fractions
common to the pararickettsia group,astudy was carried out
among other tests also on the Weil-Felix test in some miyaga.
wallas (benign lymphogranulomatosiss trachoma and primary
atypical pneumonia)"
The investigations showed that the Weil-Felix reaction
is positive in trachoma, irrespective of the stage of the
disease, in benign lymphogranulomatosis, especially in the
inguinal forms, and in a great number of atypical primary
pneumonias.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
.~67
590-- N.CONSTANTINESCU, C.DRAGOMIR, E.DUCA, A.VITA,
A..DARINQA , D.CIOCHINA. Experimental investigations
on " . " fever in Moldavia:" St. cerc., inframicrobiol.,
microbial., parazitol 1951, 2114,99
Several strains of Rickettsia burneti were isolated
from foci appearing almost simultaneously in the month of
April, in two areas in Moldavia. The rickettsiae reproduced
the characteristic infection in the guinea pig and were cul-
tivated on the yolk sac of embryonated eggs. Cross immunity
reaction establisho,d antigenic identity between R.burneti
in Moldavia and R.bur?neti isolated in other regions-,of-the
country.
591- Conf.A.STROE, C.MUSETEANUq V.CIOCIIfTEU, G.MARINESOU, .
Sp.MIRINESCU. Typhus fever in Children. Report read
at the Section 6fr'Medical"Sciences"of'. the ~R.P.R. Acade-
my on December 30,-1951.
Clinical observations and laboratory data obtained in
393 cases of children suffering from typhus fever during the
1946-1947 epidemic, confirms the opinion that this disease
is usually benign in children: moderate fever, short dura-
tion (less than 12 days), discrete or no eruption(15% of the
cases), few respiratory-and cardio-vascular complications,
moderate neuropsychic signs.
Splenomegalia was observed in 6o % of the cases, en-
larged liver in 25 % of the cases, abnormal urine analyses
in 2o %, physico--chemical modifications of the cerebrospi-
nal fluid in 78 % of the cases.
The Weil-Felix reaction was more often negative than
in the adult. N? lethal cases were reported.'
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 CIA-RDP80T00246AO19200640001-8
5920- A0STROE, C0MUSETEANU$ ?"OCIOCII/EU9 G.MARINESCU,SP-.MA.
RINESO'U Clinical and laboratory study of Bole
fever patients during the 194 1 7 epidemic. Report,
read at the Section of Medical Sciences of the R.P.R.
Academy,on December 3ds 1951.
A detailed clinnco-statistical and physiopathologic
study was carried out on a group of typhus fever patients
(8ol cases),during the 1946=1947 epidemic. New data are pre-
sented in connection with blood pressure ...and.modification of
the cerebrospinal fluid during typhus fever. Moreover, the'
statistic shows that the fever lasts 14 days, as described
clinically,only in 15 to 2o% of the cases. Fever does not de-
crease suddenly, but slowly in 3-6 days in 8o% of the cases.
The eruption apDears in most cases on the 5th'day of. the di-
sease and is often absent in severe cases; it can ' aptear on..
the.face. palms and soles, as in Mediteranean fever ( 13 out
of 8ol cases) .
The authors describe a syndrome,the so-calles "pressor-
effect" on the 9th day in some of the to tients presenting
before death a rise of the blood pressure together wit .
aggravation of the neuropsychic symptomatology.
5930?N9CONSTANTINESCU,C.DRAGOMIR,E0DUCA,M.DUCA,Z.CARAMAN,--.
AOVITA, E.PAI,ADEC. Strains of _meningo?neumotropic viru-
ses isolated in man and micee Bul0Stiin#;.Sectia Med.
1952,...4,.1, 321._._
Virus strains from patients with lymphocytic meningi-.
tis and from a mouse, caught in the home of one of these pa,
tients,were isolated on guinea pigs.The.pneumotropic activi-
ty of the virus was made evident by wide-spread foci of ami-
crobial monocytic pneumonia, as well as by inclusiogenesis
with 'jasophil inclusions in the cytoplasm of the hepatic
cells. The totality of these properties together with exclu-
sive culture on the yolk sac of the embryonated egg, made it
possible to correlate these viruses to those of the.miyaga-
wellae family, the psittacosis-lymphogranulomatosis-pneumo-
nia group.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
A . P, !T.' rll r,1
592',-..R.BRAUNER, H.LACK, H.ANGELESCU, V.NICOLAU, A.DEREVICI,
A.CIUCULFSCU,-D..SARATEANtJ I.PANDELESCU, M.ERACLIDE*'
T.PASCU, I.SANDU. Study of acute'"pneumopathies,Works
of the Second session of the Medico-Pharmaceutics Insti-
tute, Bucharest, 1956, 90.
In order to establish the etiology, diagnosis, therapy
and prophylaxis of certain acute atypical pneumopathies, the
Hirst test, cold agglutination, the Weil-Felix reaction and
hemogram are applied besides other diagnostic means.
In most cases, an influenza etiology was established,
with a'peak incidence in autumn and spring. The influenza vi-
ral etiology of certain fibrinous pleuresies'-was likewise
established`;
5950- C.IACOB._Beni inoculation phoreticulosis " Protle-
me ? de Infrariliorobiol" EdMedic, BuoareOti 1956; ; j~.
Recent data are given on this new nosologie entity, for
which R.Debri_proposed the designation of " cat's claws di-
sease " and P.Mollaret " benign inoculation lympho-retieulo-
sis. ", "in 1950, A description is given of the epidemiologic,'
etiologic and experimental aspects, symptomatology, clinical
forma,positive and differential diagnosis, histologic modifi-
cations, and ending with therapy.'
A fact stands out in literature: that anamnestie inves-
tigations of the 300 cases reported up to 1954, establishe d
in 83 % of the patients a single or repeated contact with
cats. Relatively frequent epidemics are reported in one fami-
ly or in a building, showing that the cat is a virus carrier
and eliminator , without however presenting a manifest sympto-
matology. The possible geographic spread of this new viral
disease is' very great.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 :.- .
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
37o
? 5960 A@GIRCU), G,MARINESC,-,. Ocular and nervous reactions
caused by infection of neorickettsiae or elements of
psittacosis group into the anterior camera oculi?
C.R. des s4ances de lSAcad. Sci.1957, , 2433-2435
Inoculation of a virulent suspension of neorickettsiae
into the anterior camera oculi of the rabbit gives rise to an
almost total infection of the eye with lesions, particularly
at the level of the cornea, ocular choroid and papilla, The
reaction.. of the nervous system is manifested by meningo-
encephalitis, with marked vascular modifications.
597e- P.GIRCUD, G.MARI ESCU. Hepato-nepritis in new-born
rabbits-due to neorickettsial 'and certain' elements
of the psittacosis group, _ C ?R. de( Sdances de l'Asada
Sci?_ 1957, 16 2566,
The harmful activity of neorickettsias, multiplying
in the'animal organism, is manifested by various deposits
at the level'-of the blood vessels and viscera.%. (lung, heart,
liver, kidon~,1 , spleen, brain). The most important histopa-
thologic modifications were fund at the level of the kid-
neys and liver, with prevalent degenerative lesions* which
indicate the toxic action of certain neorickettsial strains.
598.- N.DRAGANESCU. Studer of inclusion-like eosinophil for.
mations within'"the cell cytoplasm in experimental neo
rickettsial. pneumonia,.. ComunicArile Acad,R.P.R. 1958,
~'g .1 l.. ?. 23
Applying the Mann staining method, the author describes
in the o .plasm of the alveolar and mononuclear cells in
neorickettsial pulmonary infection eosinophil formations
closely resembling inclusions.
The presence of these forrmations in mononuclear and
nervous cells, in experimental neorickettsial meningitis in
rabbits, leads to the assumption that they are the final re-
sult of the struggle between the cell and the inframicrobial
germ
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
? 371
Experimental neorickettsial
599. N~.p~RAGANESCU, HUNG TA4. E~_____ menin As in rabbits: Comunie 6rile Acad,.R.P.R. ,1958,
8, 129 1353:
It was possible to reproduce meningitis,by suboccipi-=
tal intraspinal inoculation to rabbits of a lung suspension
from mice experimentally infected with a neorick?susstrain, isolated in Bucharest', i
An infiltration with mononuclears and polynuclears observed under the microscope at the level of the meninges.
Some of.these mononuclear cells present intraeytoplas-
mic eosinophil formations, identical withc- those described
in the cytoplasm of. alveolar and mononuclear cells, in ex-
perimental. neorickettsial pneumonia. Perivascular lesions,
inflammatory nodules and the presence of eosinophil forma-
tions within the cytoplasm of the nervous cells is observed
at the level of the cerebral parenchyma, subjacent to the
meninges`:
6oo.- N.DRAGANESCU, D.S .` EANU? Serologic identification
erm ., isolated-from" a
of - a virulent Ae orickettsial! perm-isolated-from"
rami=
human case of ati~ is al .. np eumonfiaSt:. c e re 3af
crobiol ?mierob of "OparaZitoi., 19589 # 19' 61'
Positive micro-agglutinations performed with antisera
prepared on rabbits against the Giroud antigen, as well as
the morphologic characters and cultures of the virus,, fully
justifies the assumption that this germ isolated from a hu-
man case of atypical pneumonia belongs-to the neorickettsial
group described by Giroud.
e resense of neoricket-~
6o1'.- N..DRAGANESCU, D.SARATEANU., Th ...r L2r_e se
trial germs in the Rumanian" People ? s Republic.. Study
of a neorickettsial strain isolated from- a humag case
_- =
of acute pneumopathy. Report read at the" Second Cog
ference of the Czechoslovakian virologistsq Smoleni-
ce?Bratislava,Oetober 14 -17.,1958.
A study was carried out on a viral agent isolated in
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
372
the R.P.R. and cultivated L yolk sac of embryonated
eggs and intrapulmonarily in mice and the cotton rat, inoc u-?
lated by intranasal route.
The round or. oval isolated germ was stained red or
blue by the Macchiavello staining method, on smears from the
yolk sac and lung impressions from mice and cotton rats.
The positive serologic results in the micro-agglutina-
tion reaction show that this virus belongs to the neoricket-
tsial group.
From the microscopic view-point, the prevalent lesions
in mice and cotton rats inoculated by intranasal route are
localized in the order of their severity, at the level of
the lungs, kidneys, meninges and liver and are constituted
of lymphocyto-polynuclear infiltrations, stasis and hemor.-
rhagies. Inoculated intraspinally to the rabbit, this germ
causes meningo-encephalitis.
The eosinophil formations in the cytoplasm of the al-
veolar'and mononuclear cells in experimental neorickettsial
pneumonia in mice and cotton rats, as well as in chick em-
bryos inoculated in the yolk sac, seem to be the final re.
sult of the struggle between the organism and the germ.
6o2,6- P.GIROUD, G.MARINESCU. Oculoenc o,1t tics lesions
caused-by various neorickettsial.strains`;,' Bull. de
11Acado' Nat. Mede.958, 1421 5-6, 135.
On the basis of certain clinical observations regarding
ocular complications in several diseases caused by neoricket-
tsiae, 16 rabbits were inoculated in the anterior camera ocu-
li, uni- or bilaterally, with various strains (X14, V14, L179
and Q18) isolated in different regions, from cases with the
most varied clinical syndrome..
The clinical and anatom.ccpathologic study showed the
presence of a quasi-total ocular infection (kerato-conjunctik.
vitis, uveitis, optic neuropapillitis), with fairly important
vascular meningo=cerebral reactions.
The toxic action of neorickettsiae multiplying in the
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/1.2/06: CIA-RDP80T00246A019200640001-8
m 373
the zeningo-.cerebral-modifications.
anterior cameraY oculiexplains both the, ocular lesions and.
On the basis of this study, an? analogy was ' drawn bet-
ween the 'ocular and nervous lesions observed in rabbits, and
the loculomcerebral manifestations observed in man during the
course, of._the:.:_diseas&?
1958; :.I42V"i7 18~' 77e :'
types` of n` eorick'ettsial' strainrs; ? evidence of hep tone`-
phritiein ne.*-.born-rabbits eBuli ~de`,lAcad Nat Med:r
603, P.QIROUD,'`' G MARINESCU , M=?me `N.DU . ' Toxicity` of two'
Inoculation .to new, on and suckling rabbits of, various.,
neorickettsial strains, isolated from sick animals and .peop;le
tiind cultivated inlungs ' or the yolk membrane of the,, embryo-
it
ted' egg, gave rise to ''important hepatic and renal lesions.
similar to those observed in renal pathology.
6o21-.= ? D.SARATEANU9 N.DRAGANESCU, ' R.PORTOCAI,A, M.CEPIEANU,
N.IONESCU. Isolation` of a viral' agent -'at ' the:'limit of
the r etts rou from a--case`.'of"at pical ieumoniia .
St, c e From the sputum +.
of~a patient with iatypical pneu monia, a
viral agent was- isolated' by" Inocul ation into the yolk. sac of
7-12 day embryonated eggs, maintained at 37o and334?C; they,.
Are' able to transmit to mice. by intranasal route a serial,ly.
transmissible lethal disease:
f th M ebiavello staining method --polymor~?
a
B
+-"k.0 -non['-1rt~ttA to OrouDr
y means o
phous' corpuscular`"formations1 2oo-5`66 in size., staining
red and blue, were revealed`on'Ythe ti wears obtained from the
yolk sac of? the inoculated eggs; and ;on mouse lung: impre$-,.. ;
sions on the basis;. of serologic examinationsof the patient`
and the morphologic orl cultivation charactersof the virus,
it is' considered that the yisolated age msy ,be classed. in
? K ~ .l "? _. i .....t v ~.. ... ~ V ...~) ~ ..?, t ... 7 v as
L Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
374-
605.- D.SARATEANU, EL?OPRES T., N.D.RAGANESCU. Isolation of an
inframic.robial agent from the Psittacosis-ornithosis
group during an epizootic in ducks. St., cere.,. infra-
microbiol.smicrobiol., parazitolo. 1958, 11, 4, 417.
During an epizootic in ducks a'virus was isolated which,
inoculated to mice by intracerebral, intranasal and intrape-
ritoneal routes induced a lethal disease. In the smears from
the lung, spleen, liver and peritoneal exudate, the causal
agent appears polymorphous, staining red and blue by means
of the Machiavello method and localized in the forffiv of intra-
cellular particles or extracellular clusters, Inclusions are
to be found in the cells of the alveolar epithelium.
The pathogenic and morphologic characters of the causal
agent led the authors to list this virus in the paittacosia.-
ornithosis group.
6o6.- D.SARATEANU, N.DRAGANESCU, R,PORTOCALA, N.I,IONESCU.
Neo rickettsial 'pneumonia. Isolation-and--ideiitification
of the causative went. Miorobiol., parazitol., epide-
miolog,,1958, .. 6, 529,
A case of acute pneumopathy is presented, from the spu-
tum of which a germ was isolated, serologically identified as
belonging to the neo-rickettsias described by Giroud and asso-
ciatese
This germ inoculated to mice by intran.as4l route, indu-
ced pneumonia with microscopic.lesions due to lymphocytic and
polynuclear infiltration and necrosis.
6o7.- D.SARATEANU, N.DRAGANESCU, EL.OPRESCU. Isolation of vi-
ruses other than the influenza virus in'pulmonary s
dromes simulating influenza. The Third-Unional Conferen-
ce of Influenza9 - 25'_ Sept; 1958, giew.
The investigations carried out Confirms the participa-
tion of the ornithosis virus in the etiology of atypical pneu
mopathies and draws attention to a new group of viruses, the
neorickettsias, which can induce the same clinical syndrome.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
375
6o80- R.BRAUNER, N.UCK, N.BUCUR, D.SARATEANU, N.DRAGANESC1J.
Considerations on some cases-of ornithosie''St. cerca,
inframicrobiol., 1959,.2. 1, 31'
81 cases of atypical pneumopathies, 8 of which are
considered as ornithosis pneumonias and 25 'as suspect of
ornithosis, are described.
The clinical aspect of ornithosis is presented and
attention is drawn to the existence of ornithosis cases in
our country,
6o9.- PRO.F,V.BUSILA, I.VASILESCU, A..FITARAU, I.MONTAG
(Clinica de Boli contagioase- Timigoara);
D SARATEANU,-EL.OPRESCU-MSSIEVICI, EL.NASTAC (Insti-
tutul de InfraMicrobiologie, ' Ace,d:R.P:R Bticure$ti).
Contributions to the clinical-study of ornnthosim. ,
Microbiol: para~itbi:~"epidemioi 1959, 3 132;
The case records presented illustrate the diversity
of the' clinical forms, the intensity of the pulmonary spn..
drome and the difficulties of differentiating, on the basis
of clinical criteria, ornithosis from other viral pneumopa-
thies.
Diagnosis may be elucidated by means of serologic
reactions or by isolation and identification of the causal
agent. In the cases described, diagnosis was made by means
the complement fixation test.
61c,e- D.SARATEANU, N.DRAGANESCU. Experimental transmission to
the cotton rat of a neo. rickettsia) germ isolated 'from
a human c a4e of atypical pneumonia,** " .. Acta. Viro logic a "
1959, , 1; 46...
It was possible to transmit lethal neo--rickettsia)
pneumonia to the cotton rat (lethal 95 hours after inocula-
tion), by intranasal inoculation of a suspension of yolk
sac or lung of mice which had died following the experimen- .
tal disease.
Predominant microscopical lesions were to be found in
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part-'
art - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
- 376
the lung and consisted of inflammatory, oedematous and con-
gestive lesions.
In the rest of the oz} ...._j s lesions of circulatory or-
der were to be founds the inflammatory lesions being discre-
te or 'absent.
By means of Giemsa staining, the presence of neo-ricket-
tsial elementary bodies was revealed both inside the alveo-
lar and bronchial cells and extracellularly.
611,- D.SARATEANU,.H.TAO, Gh.MARINESCU. Experimental ornitho-
sis in hamsters.
aper_ rea _. a the Scientific Festive Session of the
Institute of Infrazicrobiology of R.P.R. Academy,
1718 August,19590
- Paper read at the Interregional Conference of Morpho-
logy Tg.Mureg9l8-19 December?1959
Experiments on 93 adult hamsters and 58 sucklings
showed the morpho-pathologic modifications produced in this
animal following inoculation of the ornithosis virus by in-
tracerebral, intraperitoneal or intranasal route"4:
From the data obtained, it results that the histopatho-
logic criterion cannot be used for differentiating ornitho-
sis virus strains
612.- D.SARATEANTJ, E.NASTAC, B.FUHRER, EL.OPRESCU, T..HUNG.
Investigations on ornithosis virus-'infections in the
Rumanian People's Republic. St. cerc., inframicrobiol.,
196o, 11, 19 73; Biull.Nauc.Inform0196o929137; Acte
Viro1.oUng?196o,Z,2,l860
Eight ornithosis virus strains were isolated from
ducks(5)0 turkeys(2), and geese(i), pathogenic for the whi-
te mouse inoculated by intranasal'route, and less pathogenic
when inoculated by intraperitoneal route. These strains were
also pathogenic for the(. chick embryo inoculated into the
yolk sac or the chorio-allantoic membrane.. The antisera,
prepared with the isolated strains in cocks, produced agglu-
tination of the specifically stained antigen and gave posi-
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
377
tive results in the sercneutralization and complement fixa-
tion tests in the presence of the specific antigen, permit-
ting identification of these strains as ornithosis virus
strainse
The morphopathologic alterations produced following
inoculation of the ornithosis strains to the white mouse,
suckling and adult hamster and irradiated or non-irradiated
white rats, were studied,'
In order to detect ornithosis infectims in mans in-
tradermoreactions and complement fixation tests were perfor-
med with the sera of patients presenting a clinical syndrome
of PAP, caretakers in bird-breeding farms, the personnel of
the bird slaughter houses, as well as pigeon and parrot
breeders, etc. These tests yielded a high proportion of po-
sitive results (3o.9%-..833), demonstrating the wide spread of
ornithosis infection`in the country. Intradermoreaction is a
sensitive, early and specific test, that permits detection
of the infections even in the first 5 days of the disease.
Complement fixation is more sensitive in human cases, and
the authors stress the necessity of using double or even
triple serum samples in order to obtain conclusive results.
r013.- I.BERLOGEA, I.STRATI, S.SCHOENFEID, D.SARATEANJ
GH.MXRINESCU; W.T.LIAN: Data on ornithosis infection
in the ' irradiated and non-irradiated white rat'*' St.
cerc-e inframicrobiol. , 1960,E 11, 19 111.
An attempt:was made to transmit.ornithosis to the
white rat, a species that is resistant to this infection,
by using ionizing radiations in order to overcome the na-
tural resistance of the animal. The investigations were
caried out in 47 white adult rats, that received a dose of
51orrat each exposure. After 3 days, this rats were inocula-
ted by intracerebral or-intraperitoneal route with duck or-
nithosis strains,
The experiments showed that the lethal disease cannot
be transmitted to the irradiated white ratj,, which presents
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
=378-
no c linic a.1. k . _,~1m the d::_.: e ? Complement fixation with
the sera of irradiated animals yielded titers twofold lower
than those of the non-irradiated animals. It is considered
that the dose of ionizing radiations used was not sufficient
to modify the cellular systems in the rat, so as to satisfy
the synthesis requirements of the ornithosis virus, for re-
producing the experimental diseasee
614?- V.TOBUSIIA, R.ALEXANDRESCU, D.BACALOGLU, D.SARATEANU,
EL.OPRESCU-LISSIEVICI. Clinical and morphopathologic
aspects in an mic dtae to! ornithosis virus St.
cerc., inframicrobiolm, 196o, 119. 2 _ 187 '_ ,...._ _
Investigations were carried out on an epidemic due to
the ornithosis virus that broke out in a duck breeding farm,
affecting the 3 caretakers and the watchman. Subsequently,
to these cases.another case was added - the mother of one of..
the caretakers, in which the infection is explained by direct
contact. The clinical diagnosis was confirmed by complement.
fixation that was positive for all the patients, and by iso-
lation of the virus. The epidemiologic survey showed that
the..epidemic had been preceded by an epizootic among the..
young and adult ducks. A single human lethal case was regis-
tered; the work relates the more important morphologic as-
pects observed at necropsy and at the microscopic examination,
615.- D4SARATEANU.,.E.NASTAC, V..T..BUSILA, A.ALEXANDRESCU.
Laboratory study of an epizootic due to,the ornithosis
virus. followed .by.....a human, epidemic. St. cert, infra
microbiol., 196o, l.1, 20 195.
_ .__ .,The_oxisteiice of.-.6h.ornithosis-..epizootic--in..ducks.-,
followed..by._.5. human. cases, is reported, showing once again
the possible transmission of ornithosis from birds to man
and from man to man.
The presence of antiornithosis agglutinating antibo-.
es in the sick, convalescent or appa;ently healthy ducks,
and of complement fixing antibodies in the sera of human
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 !I I
379
d~~"'1 I" ems.. u..,_. ~Y,. ,..4 ,4, .: ?? ~.. le ,.
Iogyi,of the~"epizoot c aud' epidemic studied;o:t.,
cases, as well as isolation of some virus strains from birds.
~, ?, r. ~: ,- r ; fit'
.
and a lethal- human'case,
;_, -~po;in"te,rd,' to a common' ornithosis etio-
'
616.- G.MARINESCU9 D.SARATEANU, T,HUNG. Comparative studyA...
of 'fhitolag alte'raticris `in white,rats inoculated with
_ "various auto'chtonous ornitnosis'. strains. St.: cerc,, in-
framicrobiol '1960 ? ll '2 201:
The authors describe ,.the ,evolutive .:aspects. of, then his-
tQpathologic picture followinoculation, to.:the.. adult wrhte:
mouse of 3 different ornithosis strains isolated in the.R,P.R*
by
various routes..
. Inoculation .of these, strains by intranas
;al route. indu-
ces character ftstic`ornithosis ,pneumonia. foc i. The TC strain-
(turkey) gives rise sooner to interstitial, andalveolarlpro-
cess'es-=C3' t6-1 hours) than the'CR ' (duck) end TG(
o V
? The r stra
ticulo-eadotheiial system-were noted. at" the level of the
the v.rhite mouse 'following" `inoculation by iltraperitone al rou-
to'o - Stasisa degenerative lesions, and `hyperplas a of the re-
.d" manifest a low patnogenici` v for
phocytc''menngo-encephalitis even after intranasal inocu
Intracerebral inoculation produces lethal 'meningo I en-
aephalit:s The' TC 'strain (turkey) produced serous or lym
'Element'ary bo'dies were found''in all' the organs:, lung
(infiltration molionuc'lears) 9r liver and ''spleen '(reticuloen-
(alveolar cellso bronchic cells and macrophages),'b?rain'.
61 -' DoSARATEANU'9 'T~, UNGy Go IEESCU. Experimental orni-
1-29 1-29-169,"
Three ornitho,sis strains were inoculated,,-by,intralna.-
i.ntraperitoneal and 1ntrac.erebral route to. 93. adulat ,
96ol.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80TOO246AO19200640001-8
380
and 58 young hamsters. The 7e tilts obtained point to the .
possibility of transmitting the lethal disease only to young
hamsters and only following intranasal or intraperitoneal
inoculation.
... The present paper describes the morphopathologic alte-
ration$ produced by inoculation of the strains by various . .
routes The histopath.ologic criterion cannot serve to diffe-
rentiate the strains.
Irrespective of the route of inoculation used, the
strains presented marked pneumotropism.
6l8b= TeHBNG. Experimental and moophopath.ologic inves-
tions in the psittac-osismornit o is g. roizp.4.
Ste cero. inframiorobiol., 196o, 1~,, 3, 425.
The present paper deals with four experimental inves-
.a.
tigations carried out in the white mouse, adult and young._
_ .
hamsters, chick embryonated eggs and rabbits, with the o'nii
thosis virus strains (OR, TO and TG) isolated in Rumania _
The various staining methods used and studies in the optical
and electron microscope led to the following results:....
In the development of the ornithosis~ virus it was not..
possible to confirm the existence. of the evolutive cycle des-
cubed by Bedson and other authors, From the moment which
the virus penetrates into the host cell up to rupture of the..
cell, the.. virus is to be found in the form of elementary bok'o*i
dies, that can be modified, agglutinated, enlarged by the de-
fence action of the cell.
The presence of inclusions proper was not observed,@_
since they were formed of individual elementary bodies. In..
the electron microscope, large elementary bodies with a lO
density and small bodies with a higftdensity, . were observed.-.
Apart from the cytoplasmic ?'alterations, examination in-,
the electron microscope also showed alteration of the nucleus.
The presence of viral elementary bodies in the endothe-
lium of the small blood vessels and infiltrative elements,
proves dissemination of the ornithosis virus by hematogenous
route
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
Intradrermoreaotion is a sensitive, specific and early
test, which can detect ornithosis infection in the first 5.
days of-the- disease. Ae iplement fixation is just as species
fiat but not so early or lasting as intradermoreactiono
ornithosis strains.
-Marked pneumotropism of the strains studied was obser?
ved , irrespective of the animal or route of inoculation used.
The morphologic criterion cannot serve to differentiate the
o
619. D.SARATRANU,, '-psittacosis?-~ornithosisoGene ralities his.
tors, eharaoters of the viruses methods o~ diagnosis.St0
certo inframiorrobiol. a, 196o v 11, 3 p 445
The author reviews the data in specialty medical literate
tire referring to infections produced by ornithosisppsitteieo4
sit germs, and especially to nomenclature,, taxonomic clas j
fication and history of the disease in mane
The morphologic and Immunologic characters of the viru-
ses of this group are described, as well as the methods of
diagnosis. The value of various diagnostic serologic tests._
and the way in which the results should be interpreted, are
discussed.
The economic and epidemiologic importance of pararicket.
taial infections in various mammal and bird species demands
the organization of a systematic study of these affections in
our countryo
ced by pararieketttsias in various mammal speciea'o.
62o.- C@SURDAN, G.8ORODOC. Infections in mammals produced b
inframicrobee of the pararickettsial group.
8ta cerce~.
inframicrobiolo a 196o, 11, 3;; 4590
-----The-most_.recent bibliografic data-regarding infections
Produced_by_germe-.of the- psittaeosise ornit ..psis.lymphogranu.
lomathosia-group are.reviewed.-The-authors-briefly describe
the anatomocclinical,, ?pizoatologic . characters- and- methods. of
diagnosiao,,and...tho-control ? and prevention-of-diseases product
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
General considerations are made on unification of -.
the e tioiggic nomenclature, the best being that proposed by
Aoa&.oSt0SoNicolauo Opinions are likewise stated concerning
the designations given to the various morbid entities des.
c '4bed by different autho 5 in the same or different species
which have factually the same etiology The authors raise
the proble of mixed pararickettsial and bacterial infec4
=
Lion; fl that have been considered up to the present as ha
vies a bacterial etidlogye
In the investigations wader way, an inframicrobial
agent with characters e3 la to those of the pararickettsias
was isolated from cases of swine enzootic pneumonia, Similar
ger were also isolated from cases of eua and calf
pne a.oentv. 'i.t ,a U
621oa DOS.ABATEAMU9. NoNASTAC Bok?tJHRERO. EL.OPRESCU9 C.T0HUAI9
12e1 C o C aratiye investigations on the diagnostic, vale of complement fixation and of intradermoreaetions
t ors i,thokav-'
196o, 3.. 2530
Mierobioloo parazitol?s epidemiolo e
By using 3 ornithosis strains, in intratracheal moon.
latton the authors induce disease in rab'bits' in Which. they-...
s,ubsequc~ntly test the comparative value of the jntradermoreae
tton (IoDQR?) and complement fixation teat (C.F*i' 0. Aceor
ding to the data obtained, in 7 human cases -1nYestigated--__
there. was--a--concordance of - ?2,. between the CeJT.- and the
l 4,DvRo ...while -yin the experimental :vest,igations .the --concor-
de oe-was of .14e.%%o--The_.CeP T.. a .ume..to-be .more-sensitise
than the .L.l ,,Ro .. in .h ca e fl ity. inde* ofx 5l.*$
being obtainied with the former aiad o'- wit; the latter.-
'The experimental investigations ~hh;ow d the 116De2r to be _a_ .
sensitives specific and early test-: . affords the possi ?
bility to detect the infection ; as a.aof. as during the first
5 days of the disease a Repeated intradermal inocuiation- of
the antigewl does not cause the formation.-of coleme t fi-
xing antibod_iseco In a sensitize or an :i .p, tbo o ?.R may
play the role of a booster inocuiatic no The *:Ce,F.,To is just
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
383
as specific, but less early and persistens than .the IOD.Ho
6229b D,BARATE NIJ, T.HUNG, BoDUMITRE8CU, NoI oIONESCU.
of the behaviour and mor ho atholo c alteration _pro.
duped b ornithosis etrain? in the chorio-a lanto c
membrane of the developing chick embM~, Sts cerc0 in_
framicrobiolm, 1961, 129 It 830
Investigations were carried out on the behaviour axtd_
morphopathologic alterations produced by ornithosis strasi
in the chorio-allantoic membrane of the developing duck embryo.
It is shown that propagation of the ornithosis infection to
the unaffected tissues also occurs by circulatory route?
Histologic alterations are initially due to the toxin
and subsequently to the action of the virus.
The histopathologic criterion cannot be used to di-ftsren~-
tiate the strains with a low virulence, inoculated into the
chorio.-allantole membreaws .
- - The presence of viral elementary bodies; of various as-.
peets and sizes warn demonstrated on thin sections in the elec.
trot microscope.
6234,m ACAD.BT.81.NICOLAU o 9ardiova ulcer Ifections induced
b~ i a .crobese at ceres inframicrobjolo, 1960$
49 619p
- - The author relates the observat ons0 experimental interpretation8 and Conclusions-of prof op. Gjroud .
and co, Wor ra,
in _tha.prob1em-of..caa+diovaecu a affections produced by in ra
microb :The works.`of-prof P..Giroud have proved-that'" nume _
roue-cases of thromboarteritis._obliteraae., and..cardiac;;:,infarct
are caused bar ricketteiao.=pararickettsjas and,
neorickettsias.
.1;~ L.. e. sp demiologic . clinicalaand.histopathologic - "ind s'
ands ? cpepimontal data refesrrin~g. at' :the :anal et oa and~ider tif"io
cation of inframicrabiaL germs, V and the results of.s olog . .
investigations in various card.ova,ee are affections b a .?,~ ,
ria?d.- Ora ohs b~ssie~; o the~da a. reeented.'-the au d 3 =ma ee
a series of ad8. 'trio nah considerations on the importance of
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80TOO246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
the tests usedy. interpretation of the results# t
therapy and wide prospects of the investigations
in tb..I fields which will contribute to the preservation of .
health@ recovery of numerous arteritis patients and the pre
-vention of cardiac infarct or coronary thrombosis, affections
that are often fatal,,
at stimulating the discussions following which definite
624,0- Vo TeBUSILA . D SARATEABU m OniheYM~wY.?prevention of ornitho=
aY.~Yw ~M4 Y~ .Y .W. . ^ Mi ~Ir~~,G~
si_. deduced from a izootolo ii an:d a idemiolo i data.
Mierobioio parazitol.% epidemiol?, 196o, 5e 4o5?
The data in literature on the epizootologic and epidemio-
logic aspects of ornithosis are reviewed. After analysis and
discus@ion of the means of control, certain measures sioe_re-
comets d that might interrupt the infective chain, These-_
measures are addrease : to the source of infection.,, the recep-
tivre kmss and, interruption of the routes of dissemination. .
The proposals are deductive to a great extents there.---
f ors theoretical and perhaps difficultly applibable,, -but -they
novc will be established'
. 6250=1 D SARATEANUa O. SU'RAANQ GeSORODOO0 B.P'U .ANAGNOBT3w .- _ _
sol at.3o of infamicrobe from~ a IYIfocourlilYlYof .-ewe-enzostic
~Cp? 4q .wY YY.rF.-tiia.r 7YI4r111 Y~i1.~IYY M~.~pi-+~.Y Yr~11IriYeborti ono Stv came Infrsmicrobio]. *v 1961e 12a1f, 95+
MMM.
. _._ __An . inframic,rcbe was isolated c oneomitt'antly with Sal.
moxaella_ abortus_. ovia from a focus of ewe 'en? ootic abortion ~-
The_.i.nfr .microbe- grows _in-the yolk sag of the .' -days old-chick
embryo and-is-pathogenic .?. fqr the mouse inoculated by intrana-
gall or in'tracerebral route,
In smears from the yolk sacs. and from mouse organs, _ -.
staineL by the Macchiavello methods the intra- and. eztraee1 _
lular elementary bodies have a polymorphous aspect, are large
ex4 stain red and blue
.The epizootologic character of the disease, the morpho4
logic o tinctorial and ims.unobiologic properties of. the isola-
ted framicrobea led to the conclusion that it was similar
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
to the ewe enzootic abortion virus, listed in the ,rnitho-
sic poittacosis group.
626.- AQAD? BT,S,NICQLAU,, . C.$URDANa n.S4RATEANu, Pf.ATHANASITj,.
B.FUHRSR?-ANAGNOSPE, C ILIESCU, R.RADRSQU. Viral. etiol4.?
in the field of car ovascular affections. I.. Xa~ola?
1103M Of viruses froze the blood. of patients with cardie-
~~gr,~MIYA,rYr~~
vascula - *ffeattioni. Sto cero.,c inframicrobiol., 1961
12 m 3. 275.
For the first time in specialty literature,, the authors
describe the isolation of three viral agents from-the-blood
?_
of patients with various cardiovascular affections (Reymaud's
syndromes throabo. angiitis obliterans, cardiac infarct) _~ _.. .
The isolated strains presented common characters, eial ?
tar to those of the Rickettsia and Farariakettsia, demonstra..
ted by cultivation in the yolk sac of eabryonated eggs: patho-
genicity for the white mouse and guinea-pigo largo size and
stainability by the Giemsa or Macchiaveilo method'*
The-serologic investigations showed that two ssitrains_eg-
hibited en.:.. antigenic structure common to the or. nithosis vi-
ruses# and the third strain was closer to .R.prowazekj.
6270- D,SARATSANV' f '? ;, g G.SORODs , B.ANAGNOSTE, D..DUMA i
M.MARTA, V.CUPSA: Investigations on an ornithogiss ,pf,
zootic in canaries., accompanied by human cases. 8t, cere,
iiaframicrobiol* * 1961, , 3, 347.
.. - - ?- Fr+.+.ri v 1;y V aalYCL.l as i ,.,a
During an epiaootie among canaries (Serinus canaria)9_a
virus was isolated which was listed,, in view of its biologic,
morphologic and werologi? characters, in the group of avian
ornithosist viruses.
The isolated virus way also pathogenic for the siskin
(Frigella carduelis spinuc), goldfingh (Carduelis elegans)i_.--.
greenfinch (Chlorin ohlori chlori #),. linnet (Carduel- IM s anna-
bina) and giail (Ceturnia aoturnix).
The three caretakers and the other canaries, contracted
the disease following contact with the sick birds.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
? 386 c'
628.- MARATRABU , G.BORODOC s CAURDAR, B,PU -ANAGNOSTZ.
Bovine ornithoais bronchopneum nia auad the,,poasihil &.r.-
ties. of humus eontaminatigmoSt. care* inframicrobiol. q
19619 O 39353
From two fool of ensootic pneumonia; of CaiYesg 2 Vi-
rue strains were isolated which were listed, in view, of their
biologic 9morphologic and serologic. charaoters: ,. among the,
ornithosia viruses belonging to the Parapiokr ttsia group.
gd 1ogic investigations revealed the presence of anti-
ornithosis antibodies in 31..7 per cent of the caly 8 that
bad been sick and in 44 percent of th+atsrBe
The presence of positive reacctionS aso ag lien leis: the .. .
authors- to . raise the question of co xeider g enZootlc pne o
nia of aa3vee as an anthropozoo*oeis.
629. ?oBARATRAU 0 C,St3RDAN 9 Gc.SoROD4t W 4 GN OST2.0; A . 3ERCAit o
RI,RnSTFLe Inveeti tic son:. +r ett "ia ...abortion
of atu the a erisenta dies ,s? d atte~r is
at. active unization) a t? cove.* infrau.ero a .dl*91961,
12. 4. 4410 _
Investigation of the pathogenic aetiVity for `gta t -
sweet of a pararieketteial etraiaio isolated frogs -ep abc 1ss
showed- that it induced a minor disease followed by bo'rtio o **
premature . birth of.non-vvl able lambs and pneunoemteriti6 of? -.
the.. newborn lambs: --Premature births or. abortions; were preceded
p?s f partyam a+~
by-prod oral. signs -and- followed by ante--and
cidents. The experimentally infected ewes presented iOn t0a
wela snd.1-mphocytosis l, as well as wires a, consta l o '
00-
3o days.. Complement fixing antibodies . were found at'
me ewes 7 to 21 days after the infection,,, and. in the IOU,
after sucking the colostrum for the first tim?r. w a.
Pararickettsias were isolated from the abortus xga -B t.
the foetal membranes and the lochia,. - --
Active imaunizations which could not be owe: vows. a
clallenge inoculation of 175.9006 nice W was sbtainedi i - - - -
50
experiments with a o of per cent formolated alusin u* o' '
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
387
adsorbed vaccine ..and a silvered, vaccine,;. 6 to 20 days after
vac nation, 'the. owes presented complement fixing titers of
lj8
am l/64. . _..
th
n
e mash or their researchesithe authors deriohstra.,
?
to the
i
'
spec
fics
oi
pararickettsiae in the etiology of enn
zootie abortion of ewes.
63o O.SURDAR, D BAR TFANTJ, G BORODQQ 1'?Al'RArTASIU, B,AHAGNGB?
.1
am , I
.B RCAN, a
jRSspp . liver' mental study on .
arCL U
ket
Isis . Pneumonia of calves. cars .
inframicrobiol . 9
1961. 12 _ 1i _ Aar _
e authors describe the results obtained in e
xperimen,
tal infection of calves with a, pararickettsial strain isolated
by then also from calves 'and maintained in the iI
;bar: .
serial passages in mice'. And . embryonated eggs. The calves. devo
lop a febrile catarrhal disease accompanied bya relapsing neu?
me?emterie syndrome, stagnation of growth and progres?ive debi-
lity. From the hewatologij
viewpoint le ukopshim and 1
ymphomo.._
nooytosia were found. Anatoao-pathologicallY,, infiltrative' lym..
Phoeytlc broneho??pmeuaonia, proliferative lesions and haea:or-rh
go in the lymph nodes and spleen, hepatitis, intestinal and
_
parenchymatous nephritis and proliferative endothelitis were....
found..Bleaentary bodies. were revealed in the tracheo?bronchic
epithelium; alveolar endothelium, in the epi=thelium of the tu?
bull contorts, the -macrophages .of the spleen and liver and..the
bl?.od..leucocytes... V remi,a lasts more than 4o days. In a..s3.n le
mass was there _ a- gran- gram. negative -. uoccobacillus isolated., from. the
lung;_it_ssriousl
y aggravated the-evolution-of the, disease.
giaet e_>sero~;ogic iriveatigatioas showed that..:onlT__in, a-? single
calf werecomPleae nt.. fix ing-.antiboc ee, found after-
grok
Mob begi.aming. of the infection -The results ., obtained.... showed
that,the
atiologim::agent-of bronch6pnei omia and-eete?
tisybjf . galves is a ' pararickettsia ident'ioa to the ornitho..
sis virus.
`r j[?? ^
T'?
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
63l, AQADeSTsSJIICOLAU, C?SURD4N, D.SABATBANU, P..&THANASIUa
GsSQRODOC, B.ANAGNOSTE,, C4ILIESCU, B.RADESCU. Viral,
et olo in the sphere of card ovaacular affections;;.
II 'Viral . stud offif casse_B,, St. cero. inframisro-
biol.e1961, 4, 523
The author* dewribe the results obtained in their in-
vestigstions on 5o patients with various cardiovascular di-
seases. Forty one of these 5a patients presented a positive
serologic reaotion to riekettsial and pararickettsial anti-,
gene, the highest proportion being found to R.burneti (56.97%).
From these patients, 24.straine were isoleLted, 15 of which be-
longed serologically to R.bnrneti, 3,to R..prowazeki, 1 to R.
mgosert, 12 to the pararicketteias (Q,18), of which 2 both to
R,burut ti and to pararicketteia Q- .8?
.-The-isolated agents were cultivated in white mice and
in t b# yolk sac of eabryomated eggs. Intraperitoneal inocula-
tion~ to guinea pigs induced the appearance of serum antibo-
dies,
_ The histopathologin examination showed the presence,, in
th.-damaged-vessels,, of elementary bodies stainable by the
l[a~ :`~ ?i_avello method.
Antibiotics from the tetracyclin group exerted favoura??
bie. action in the cgses studied and brought about negative,
serologi4 reactions.
632,- AcAD.S'F.d.AICOt,AU, D.SARATRANrJ, C,.SU DANA PATH &NA81U,
B.OAGNOSTE, G.dSOBODOC a C.II,IESCU, R.RADSBCU, Eolis ant;t'odie~s `;ri,werkwr~.
an tne zootechn_ice .'fi_eld.:: $t, ce'rc. nfr" crobl`al.o
1961, 12, suppi ., 365t Rev.?Bc- ti
i...jted. 19E1; 6,..` 1?2', 105o
By ?.
performing complement fixation tests with 344 sera.....
from workers in the zoctechnicai' field or in slaughter=house?
the authors have recorded. a mean index of
positive ornithosis
reactions amounting to 4 ,':$
g 3.. per cent,
The`. highest ind ces.'Were recorded in breeders of psi.
tacine?. (72.7 per cent), pi eons ?
e g (58?5 Per sent) and palmipe.D.
des (490.o5 per cent). The authors also emphasize the impo>rtan'
ce.of mammals as sources of infection in mans a fact resulting
from.. the,, high serum positive indices recorded in sl:aughtei .
house- workers. and in those. employed in, the department of an-
ti=amine-plague products 4o .,. .
per cent)e .. ..
-.-..The -.widespread oceurrence of infections due to avian
and--mammalian ornithosis gtraina belonging to the Parar cket.
tsia group is poin
641 C .SURDAN B . D.SARATJAJU, G.8OR000C B ,FUBRER..ANAGNOS Ca' .
+yoas? iv' -G L50JLation or a virus. belonging to the
rickettsia group frQa an enzogtii of aranulous vulvoon.
vag nitis,in, cattle 8t?.oere, inframicrobi9 ..,{\ 1961,
129- 'auppl. s 373; Nev.sci.lied.1961?6? 1-21 . p 127?
From the vaginal scrapings of cows suffering from cons
tagious granulous vag.nitis a virus was isolated in egg ciil.
tures, which proved tube pathogenic for the white mouse.
The LDS in mice is of 16
`5o
The virus Isolated belongs to the group of large viru.
sea;. it is polymorphous, multiplies in the shape of antra
and extracellular col,onies,. and stains by Uacchiavelle4
- - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 : CIA-RDP80TOO246AO19200640001-8
method. A I/i? dilution of ornithosis serum neutralizes
700 3 5e in mice.
The biological,, morphological and serological charae.-
ters of the virus .solited demonstrate its relation to the
ornithosis viruseso
642 e- I oBULOGBA, i O iOB' NFELD, I.STRATI , De5ARATNAI U... _
nithos38 3nfe tion in i radia@
some data Ronctrnll. ing.
ted and in non- Irradiated whits ?mieee St, certe in-
f robiole, 1961, 12, suppla, 3819
By irradiating whito .nice with a single dosi$ of
3oo-39o r the susceptibility to experimental infection with
e ornithosis virus was increased.o - -
Following irradiation, ornithosic infection could be
induced by routes by which no infection 1s normally caused
in the case,of certain strains'
CARUNTU, L0PAUL, AOTIUGRBA. Histo aQ y
643GoIiARI1+NB(:U9 Fm
th oldie aspects in a case of -re a aced typhvus f ear b
Report read at the session of the Institute of Infra
microbiology, November 209 1964. -- . -~ ? _~
A ca?e of typhous fever, repeated after 36 yearsy Is
deseribede
The histologic lesions were predominant at the level-of
the -heart and. spleen of particular intensity in -the retiou-
lo-endethe lial..system and _ c onnectivT tissues probably linked
to the latent action of rickettsias'a'
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
X _.
Vari a
644-722
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
6440- 20 ^7OLAU,. R,PORTGC ti?,T,.&.q eCAJ41 0 Staining and mores, of the mumps virus obtained by paro t 4 u;q-
Recueii d1dtudes m4dicales? Bucarest.1949?189?
In the fluid obtained by parotid puncture from 12
muunp-n patients, It was possible to reveals by means of the.
Moron v atai.mi:ng method or by that of Nicolau with phenica
ted isamins o elementary coccifo bodies with an approximaa.
to d . eter of 12a T 0 These corpuseular formations of
equal- olse are to be found on the smears grouped ih ciusterse
On the smears made with normal parotid secretion , -
oorpuoc,lar for.tions-, may be found, but much more seldom9
of vari. blo sizes. These may e :ceptionnal.ly represent b: Lo-.
phy c Ingramiorobes or, ratbor.normal oytoplaamjc. granules,
whin4 cannot be taken for mumps elementary bodies,
inclusions in human salivary cellic. ffinmps
patients ~ and. normal rso o-.., Bul. Stiin4?Acad?R,P.R.
Secto tonedo,195o ? 2?, 50 411,
ACj ' p o , a T O COLAUO R.POR?OCA3& O L?CAJ'ALO Pmto2lasmle
In the cytoplasm of the cells found.. in the saliva of
muope patientsq it was possible to reveals by means of .the
modified. Mann staffing method, the inclusions described by.
8ohier and. Jaulmese The size of these spherical or oval
in-
clusions ranges between 2-5/1 0 As a rule ? they stain red',-
sometimes sometimes light blue or a shade intermediary between red and,
be they are surrounded by a more or less accentuated halo,
These formations are to be found in be per cent of the
mumps cases?
A comparative study of the cells found in the saliva.
and-of the epithelial, cells, soaped from the mouth epithe~
la.um in normal personae revealed the presence of these for-
mationa in 20 out of the 21 persons under observatione-
The high percentage of these inclusions in normal pore
aonsg. definitely leads to the conclusion that the inclusions
found in the saliva cells of mumps patients and described---
by 8ohier and Jaulmes as path j; ;4orr epidemic mumps, are
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
,
,e, spontaneous Q? inclusions produced by the viruses that
exist . in a latent state in the mouth of healthy persons'. '
M tIED1 s I.SAMUhh, T.SANDUL!SCU. Three mumps virus._ ._ .
et_aiaa, isolated from the cerebromepimaa. f1cid of . epide
mic mumps patients. Comunic#Wile Acad.R.P.8. 19559 199
1385.
Three mumps virus attain were isolated from the sere-
brospinal fluid--of children suffering from epidemic cps,.
Two of the children exhibited clibigally apparent. neningeal .
forms and the =third 'a cytologic meningitis. The . strains iso?
lated from the patients with _meningeal ,forms.' yielded from,,., .. .
the first passage positive hemaggintinations; the strain.sod.
lated from the?~patient' with cytologic meningitis , acquired
weak hemagglutinating properties at the second passaged
Stress is; laid on invo1vement of the nervous, :system.
in mumps,, which can onqur before -.the appearance, of the
cease, sometieesithout any.olinical sign, of meningitis,
Complement fixation .in. the .diagnosis Of muape, ? St.cere 0
647. 1, ' UEL , I.FRINDVAN,, E.WIEGL R * a T.BANDUIISCU.
,
inframicrobiol., ticrobiol., parazitol., 1957, It
387.
Antigen'V fdt complement fixation~.was prepared from..
the -allantoid fluid=' infected with, ,the autoohtonoua strain,- ._
:._..Tests_m de..'with.this -antigen on 135 Sera in.complement
fixation,, demonstrated the specificity of the antigen and
the utility of the test -rin- the.. diagnosis of mumps. The au-
thorn considered as positive ` f?i the, antigen prepared by
then a titer of 1/16 for a single test and at least a four-
fold increase for two tests, on condition that the fire,
serum should be taken in the first days of the disease.
- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
348
648 To lZDb , ZSA LO Fm EGLLR9 T.SA JL!SCUo
Comparat ,ve value of serologic tests in
St, care*., . infrjmicrobiol. , microbiol., parazitol.,
19579:89 4v 571.
A comparative study was carried out on the value of.
compler lent fixation (C0F0) and hemagglutinationa-inhibition- .
(~,A0X) on 4o sera from 24 patients or convalescents suffe-
ring from mumps and 2 normal subjects without mumps in their
past history. Parallel values were found betwe?n the titers.
of-the two tests, F'arlier more characteristic and higher ti-r
tors are obtained with C.F. Following these investigations
C.F. is considers, as sufficient and more indicated for the
serologic diagnosis of mumps. The amylasuria and.. amylas@mia
tests likewise constitute a useful and early indication'.
9.oo 1oSAMU Lo.Isolation of the mumps virus. from- the care..
A itu v of strait, 8t. ? cerc., infra
bro-spinal fluid
braes
microbiol,emicrobiolo, parazitol,, 1958, 2, 19 51e~
Four mumps virus strains' were isolated by inoculation
of the cerebrospinal fluid drawn from mumps patients, with
or without mgningeal signs, into the amniotic cavity of
chick embryos
Two strains (SI and GN) were adapted to the allanto, is
t uid. and to the anterior camera oeuli of the guinea pi'4-. - -.
The strains differed from the viewpoint of their adaptation
capacity-and-with regard to the correlation between the i.nfec-
ti.ous-and~hemagglutinating- titer, During storage. of . the.-Vim
r a_ decrease of-the -infectious titer is observed.;-.the
more _aceentuated. the _ less. adapted....is . the strain, Strain G29
adapted to the allantoic fluid,, was used for the preparation
of an antigen for complement fixation.
The advantages obtained by isolation of.the mumps
vi a, from the cerebro=spinal fluid are showme lending -
support to,the theories that sustain the neurotropism of
tdh .t virus o
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
The resen . of :aacifio, 611
e taz c ozeugclew il
6,50-o- ACAdoSTO SJHICOLAUA? N0CAJALs McSEPEANU GH,MARINESCU.
tears o rua:us ~ amd .imi~reasio af:';2'iatec~lick8 s
Works of the General" iest fic- Session of the Rol',R.
Academy., June. 2 32 1950 1339 ? ::.
Until 195o the morphology of the measles virus had
been very little studied and.the results obtained were both
uncertain and contradictoryo:T'he measles virus was revealed
on mucus and tear snare and ea the - impressions of Filatov
Koplick"s enanthema9 stained with phenicated iaamine.a The
virus appears in the form of cocciform elementary bodies.'
grouped in clusters,. measuring approximately 12o T. The
frequency. of measles elementary particles is higher in the.
first hours after the onset of the disease p particularly in
the mucus and. teara.9 gradually decreasing after 48 hourse
enanthema -ih"`meas.es7patieftB
65-le- N?CONSTANTINESCU, M.IWCJ., E0DUGAg AOVITAO , Investigate
tions on the transmission of the. - virtze' of r i ...a ea
the virus from the blond.
Le B ,. to - rabbi.lGS" IMd-_"228 of 1 e ` C tom~
OfIthe
man a,r.n .em+....a^ mrsia..sa~ r~r?rn s
yir971$`~ in ebb flat8t ".,eB'~$a^8t?- Cer6:;' Infrai 1crobiola,
zaicrcbiolm a ito1 3 2
o~~ ~. 95 ~`~e,3 a 37
The virus of German measles was r veale,d in the blood
of patients by inoculation to rabbits as nd guinea pigs only
In the first three dais .of. the disease. never lfter& In the
, ;l o a blood-leukopenia syndrome was induced with- appea-
ace of plasma-cells,, accompanied` sometimes by variable fe?
brile reactions, .The virus .cultivated ovvo in the yolk
sac gave rise in the rabbit to : thee; sa ma""b1*sd syndrome a?
-- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
4so
0" o ' ~S~a~ aet~ fl R I . , _70MICU p eaEP -ICA,
Oo ThOIU? Narvous man ,festatioV in measles. A tdmo=
tF.ol e is stt d of aor - it .,ous men Dance hal ,t ,s
Sto ce o inframicroblolo ? ticrobioi; paraz.taiO 19)2
2fl 32) 1190
Thr cerebral localization is the most frequent news
nV a gc station" in the courage of rn as1es, Rumanian me tal
literatu has not let published a more nportant work on the
hiet?pAthology of cerebral omplicatio in ffie s1eaq
Thy present paper deco; ,bea the histopatholog;ig s@peet?
Oncon tovod, in 15 caags of mOas1e6 with lethal nervous manic
fe~tst,o
e clawi a1 picture of dom linizing encephalomyelitis
with Glia1 proliferation wag Once tere .in a single eansi'
The otboW 14 cases: we characterized.by the presence of a gem
rou> arnin,oaeaeephalitia process of vable intensi; eid;..
tbelit`t ulth v? us preva1enee9 blood stasis with.leukoc a ;.
throb r' Q vascular dilatation and stension of the perjVgA.
c u.189 Rpsi;20S occupied by a serosityo These cases may be listed
t supraacute toxic type of aeaslos described by
653o- G0 I SCad O P oaTROE8Ca?1 ? N, a nervous i
dons in the course -,of mo bbs.' liml s ., as et -..Ste
e d .., i 'ra is abiol0 0 _. m4crob 01,0 ?p a~ tt~l. 9 19520
ervous accidents in the coarse of seaales seldom encotan-
tered.-a have been described in isolated cases in oar .country,
The clinical study of 31 cases with nervous complication
out of a group of 2637 cases of measlesg hospitalized in the
children? s war for contagious diseases (Dr0A.Stroe) p during
the 1945 1951 period is presentedo The av6tage inc ite of
thanno accidienta (1015 per cent) is *lose to that found in po
1.ioD litio with slight a nual-dif arences ranging between
.o44 per cent 1945=195? and 2,elg per cent in 19510
Nervous complicationo usually appear between the 3rd
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
.401 and the 7th day after the bogi, it of thc) ak athemae
According to the clinical form, thc: e erGxs 2o caseb
ene;?phaliti??9 meni eacephalitia , l raae i ;?me ph J
litiss 1 serous meningitis*
Theme neurologic nattifestatio were associated in over
half of the cases, with other coplications (interstitial oV
bacterial broncholpa eiioniag otlti ? etc o
The mortality rate -in the cases with; nervous aempliqaticn~
wan 46 per cent; as ciatizn with pulmonary co!.plication of
a viral or bacterial nature increased it ~almos tvafol1. (';.3o3
per canto)*
65 ? AC A~ O ~`1' o 0 3 tai kU 0 CQ'~1.^-"~'AI~TI 1ESC J ~l v' 3 . m ;
lei Po ??l sox;a
of t ?^ rUPI, of o lea: i VV IS arobi;ole~q mla o1 iw s o ptLt ?al1twt o ?. l9 ? I? 3 , l 9?
sine a, Ito wi, _ pea c ti ato im to the org im
by the aaopp',ja. .b X'DU+-:0 p a Rs ? at't > pt bay m t
block fir OIL of GI nta? by ractiec. OZ Pa0e v + 1"+1 +1'n'i.z:.t?oZpp
wit, a 5 3 to i.:, fecctione
k btitr 1@g, 11_ imi nizationo The virus attenuated by *on-
waleacent seg.v.,;aa it a P po:r is of l voia o virulent blo64
+ 99 volumes ae Y01 (kopt in contact . dart cg 1 hour at ?C, ..
then 2 hour at 4400) was administered by nua it in t ]1=,y:tia~::
to a group of 27 ivh: drez vdthoUt o?cbilii a?'. tece enta 0 . ataoa g
which a case of measles 'had -appe edo in -the fol.loving 3o da as o
?
no otter cane ,% reported e
Passive local i umizatiom Convaiea ent aye wane in-
stilled tr1co &..' . du t1 ' 4.. d`,8 (71 p in aeoh nostril) to
a group of 12 children, ged 1.4 ra a so, without aea le s is
their a ar%?tacc d: Mt- s:0 In a cc o , w aQ, im which a Cl se;
of sa
confounded with thought (conacienoeand: that our scientific,
knowledge of the brain Ades not .justify the inclusion of
conscience in the field of natural sciences. By the study of
this article to-dayq the authors open; up prospects for consi=dering Victor Babe$ from a :new angle,,. that of the history of
riaterialist philosophy :in -our..oountryr,, thus situating the man
of science among the first materislist thinking inteliestna1s
of the Rumanian people.
69o o- ACADoST oS.0NICOLAUa ECATAIro. a pparent g . enzoo tic dise ase>
in rabbits -due-to as 'Yunltno `fie o" Bul.." St, ACad0R.P:e ar
maf. " rabbits, reveale:4 in 45 cases ;(normal dr previous1y,A 0 -'
culated animals) the presence ofchronic :lesions characterize
by lymph nodules and perivaecularitie processes, with :-rononUe
clears.? The most interesting lesions were encountered at the
inocu.iatea win various, viruses Cana '! } anon- nocu.Latea-, TV cr
A study of lung sections from .loo " rabbits,, 5? of wh
Beet; -Ste-Redm 1958,,"? "' ".? -5"? . .
It was not possible to detect "nuclear inclusions; o#
Bible nicro-organisus in any;of the sections-6,
level of the bronchioli or- :around the blood veeselsd
The animals with these :lesions behaved perfectly 1nocmahl`.,
not presenting any morbid .syri tox ..
Study of chronic pulmonary lesions in a group of rabiitip
in wbiicb. the leukocyte count had .been performed,, showed a aer*
tain parallelism between the :;presence of these lesions-and the:
existence of monnucleoeis,9.'particularly .lymphocytoeise
These data.gave rise .to the .question if a certain rela-
tionship does not exist between blood lymphooytosis and pulmo-
nary lesions.
The growth of these lesionso, proodf _of the apparent d1
cease, eliminates any errofg .. of interpretation in the. ceur
of experiments on rabbitse,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
r ,,)l. 3i.1; 1
ch r _tg iz"ng hboj, ___ .__? 'o ki oAC dO P0PO30
nic
h ~.(1db Ra~~j~o~l la layer. JBL ~., Iayer~0 a(?i Pkonen /~~.s~~.pr'`~~^. ~la. of de/'^~
-A der ari- to F:S aipig?. i
0 r rt._, 1 ep is i.iu _ appea 'fl ao 'ok?lpa li^4.
`9 infi it ~'~d` ~1 c1 of leu. ^ r P_.,Lz pa's't cularliL.,y pot 'ug1clearoo r A-
C tio~~ I J C'wi! a al-9 fo[?.(~d artnai esr i eti'ree intraaeptt. elial g"ie o
pustleaa tttw, e bec.on4 confluent aLe, constitute the eruptive
elenQ:r t d'est:roya the co muoua epi -; e .ia 1&7,6r. that ee-
parPater i. from the exterior,
'in the skin lesion duce to the virus of in ' Mile Sueta
Cilia ciao cytoplasmic inclusic+ra are to be fou t in the epi=
N.; ~+ay. ! cello, Viral colonies or pre-inclusion " stages
((p ,abahly motor, leer, du?(,,@ nora-teda as glutinated gerc ) nay
a i ;t in the Malpigbi layer and, in the `:filtration le ooyteea
r,;` rticul R-r17 in the 14) non, w1clQa , cells, The latter in=
^,,:a ^~~topl~~ naic fo ritlticns may als? be found in the infiltra-
_'z"u'l Cc- ..a l the Amsrte,
The enGa ble of the lesions induced. in the skin by the
}w~t,a T?trs'u o because only a virus can produce such Inc lusion =
'Uffere from. t .e changes produced in the s .in by the vaacciniap)
fix-;:-pee zoJt?org si!ali pox or herpes vi .seso
So ASE CC v Econojjaic use Of food. Lo
`i> a.l . Rev. Sto Med09l.951, J uary96oo
In t %,a -'pod of laboratory animals oats are one of the
It is known that at leas`' 50 of the grains
;;:,;,~,n to ter;' aalo is scattaredo nixed with the defections and
It in prc-pomed to for i a paste with the grains a giving
za rod-like ,shapes Which is dessicated and then cut into..
lf, pieces and given to the aninals. The animals can gnaw
;?F foot up to the last bit
The authors propose to include into this paste Qrushed
and. thole oat grains fl wheat bran and water,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
t,ryU~F r+ha{;yp~f~ r~8doa(w;;A k'?_ "Cu }~yE01 S8 yo~t9 2s 9?ppn `gyt Ica Ca~t.)},$ ;.rte
al.`?.
d" Gs,.d~ L. rrho'~lA oo.. M'm !,i t' t" s, now-born, infant), St.
cg W' y > +a
riicrobio1o9 parazito1n, ~b 9393= 'dM..%~0
Investigations carried out in. 22 cases of c~pi?e~.i 4..om
r; nox rf in infants (epidemic diarrhea of the +e,a-`bo n
tc .~la er t rate ~ the presence of the influenza vl.ruo t o ?., r l;l
n-Hsu-pharyngeal washings (7 cases) and the f .ec e (8 c a ) o .
1--t was possible to isolate the virus fro,-- 9 cascs in is ,9 :tf `sue c LGrl z!_ -1.Ji1 _itd,
,n ",:,~j local
:~.Q'i. tiJ'C?F,~a, cb`.',Gr,v,,- d. be 4 v,- co s
b'L a'! -it k' ? e & ~' cab "A ty o~ :~o f_aS y' ,bra=
.15 ..e all tr'.',~ C-3.3-8. ex except G"~'
acid e of t 23 .1 cf tin L g
T FF~y q~y. e ty T'srd a C 'L~ .1)e R y. * it xr ie'
~3?i ~:; l~' c) '' p espc i.iy in t ho 'c: ?7 Za
3 i.
of a jud r"Ib', (:-
r with a v t?'..C 4!.t".'fwn ci;; of th rg
t a: ;`'c protection of the ree:1?"voaas zyo,tai:.1 a airiot
.= ' Cr;' ', : 5e5F'..o
Of fructac-'21
a^ ti`:~ ~ y Report read at the Institute of -e~s"~L ~ of the .o-_'PORO eca er s~ 5 October 1959
'Ic?ohc].: , {ant
-ho. f
Z, was prep 4. 'n..~ for GY .l b l..i arF ', ester.. a r.tb phosphoric
a~_l P11' a:::llvi m .;ion of e;zy es0 determination km(' jI.J of .i.xlpor W arc e in the o-P
: -
a,:", test - in the c ours e o
is of partiou.1a ' inn.ere ' ..n
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
- 428 -
clinics of infectious diseases.
Synthesis of barium 1,6 - fructose diphosphate
was obtained by direct esterification of fructose under the
action of specific enzymes,obtained form brewer's yeast;thus
inversion of cane sugar and phosphorylation of the resulting dex-
trose is avoided.
Ezterificetion was done with a disodic phosphate mixture
(0.25 m) and monopotassic phosphate (o.1 ni) at 37?C,up to com-
plete binding of all the phosphorus available (3 hrs); the
ester is sterilized with barium salt (acetate).
The active value of the preparation was determined by
a method proposed by the authors,using as standard,"standard."
fructose diphosphate and as specific tests aldolase catalysis
and 2 - 4 din; tro--phenyl-hydrazin staining. The indirect method
for the determination of fructose -1, 6-phosphate cannot be
applied to the biologic product, because of its content in pri-
mary esters(phosphates)
This synthesis procedure guarantees an output of 33% cal-
culated in fructose pure barium diphosphate; from cane sugar
only lo,8 % is obtained.
7o3.- R.PORTOCALA, I.SAMUEL,G.TIRNOVEANU. On the Presence of
cytomegalic inclusions in the sub-maxillary g ands of
the new-born infant. Report read at the Second Congress
of Hungarian Microbiologists ,Budapost, 22 Sept .1959.
The presence of intranuclear cytomegalic inclusions was
observed within the strongly hypertrophied cells of the sub-
maxillary glands in 4 out of 32 new-born infants, that died
from various affections. The ration of positive cases in this
study is l2*?%.which egrees with the results obtained by other
authors.
This paper purposas to report for the first time the pre-
sence in our country of the disease with cytomegalic inclusions
in new - born infants.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
vae4
7o40= IoADERCA0.Recent investigations on moasiesvirus.,.
:Jcrobiolo,, parazitol.os epidemioloa 1959, 4, 6, 481.
The author reviews the data in literature concerning
the isolation and cultivation of the causal agent of measles
in emhryonated eggs, animals (monkey) and especially cell
cultures in vitro.
The works of Endei gMilovanovio, Melnick and Ruckle
regarding the susceptibility of various types of cell cul-
tures and the characters of the cytopathic effect produced
by the measles virus in these cultures, are described...
The author likewise reviews the main works dealing
Cp4,ith serologic tests in measles, especially complement fit. .
xatioa using as antigen the measles virus grown in cell cul-
tures.
705?? ID08ARATEA19Uo borata i nom of infectious mono-
nucleosis oMicrobiolo, parazitol., epidemiolo, 1959,
4,. 2, 1750 _ _ . _ .,
-. _ .__..,The laboratory methods applied for the serologic diag=
nosis of infectious mononucleosis are described, _as well as
the presumptive tests, and the quantitative and qualitative
methods currently used. Appraisal and interpretatior of the
serologic rebults is another aspect discussed.
7o6?? G0BALMUS, I0SAMU!L, LoMIRZA, T.BNACHEO Investigations
on a severe epizootic due to paracolobactrui. in suck-
li.laboratory rats. Microbio1m, parazitol?, epidemiolo
1960, 5, 445; Acta Virolo9, Ung. 196o,.2,, 2, 1870
The authors describe the symptoms, histo~)athological.
lesions, pathogenesis and treatment of a spontaneous, seve-
re epizootic ea Used by Paracolobactrum intermedium in sucling
laboratory ratsm -
Since the paraoolobactrus group with the exception of
the-Arizona and Ballerup-Bethe..a types are considered to be
nonpathogenic, or very slightly pathogenic, it may be
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
assumed that the cold endu:r?' '.. 7,t- the anima] s during transport
and their young age interfered in the production mechanism of
tie infectiono These factors promote changes k the animal's
reactivity, permitting the development of the virulence of the
virus, which passed.beyond the intestinal barrier and caused
septicemia infection,
Certain sulphonamides.:: and antibiotics of the tetracy-
cline group, administered in the food,of the animals, brought
the epizootic to an end in a fear days,
7o7,- TOHUNG, N.,DRAGA SCU, Experimental investigations in
Molluscum contagiosum. St0 cere,infram.icrebiol0, 1960,
119 2, 2930
Inoculation of a non-filtered suspension of Molluscum
contagiosun into the chorio?allantoic men,. cane of 9 days em-
bryonated eggs, induced microscopic proliferation and intense
stratification of ectodermal cells, with an aspect of invagi=
nation into the mesoderm.
.laF~
....._Intens?_bistiocytic proliferative lesions and slight
phomononuelear. l_nfiltrations, are observed in the mesod.ern
Within the cytoplasm of the ectodermal cells, the pre-
sence of eosiiiophil formations surrounded by a halo and varied
in number and.. size, is noted0; Hire and there these formations
present a non-homogenous aspect0
_h.- ___Intracerebral .inoculation to-250-300 gm young rabbits
induces,. only_at.. the first . passage, inflammatory. lesions in-.the
choroid plexus and-the wall of.the 3rd ventricle. Within the
neurons of the cerebral parenc#Y.xa, the presence of granular
clusters is observed, suggesting the aspect of elementary bo-
dies that push the cellular nucloqu towards the periphery0
{
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
431
7o8o,. DT$T,SoBICOLIU, R.PORTOCA.L&, Recent Soviet investiga_..
tions in the field of inframicrobiolo. St, cerc.infra-
microbiol., 196o, 11. 29 317.
The present paper is a synthesis of the main Soviet
achievements in inframicrobiology. After expounding the evo-
lutioxa of inframicrobiology in the Soviet Union, the authors.
briefly describe investigations in poliomyelitis, encephali,
tie, epidemic hepatitis and influenza, viral diseases to which.
Soviet discoveries have brought important theoretical and prac-
tical contributions, such as the results obtained in antipo-
liomyelitis and antiinfluenza vaccination, histopathologic.
.,nd experimental observations in epidemic hepatitis and ence-
p:kr~litiso
7o9.- G.UANESCU-POPESCU. The action of vitamine C on the c l-p
tivvaation of certain viruses j4.4hick. embryonated eggso
St. care, inframicrobiol., 196o, 11, 3, 4ol.
A series of investigations were carried out concerning
the influence of vitamin"."Con virus cultures (influenza, vac-
ciz?ia, avian.diphteria-variola, mumps and avian pseudo-pest
viruses in chick embryonated eggs. - -?
. The results of the experiments showed that vitamin-0
in 00005 gm amounts favours multiplication of the viruses
st...'ied in the chick embryonated egg, while in smaller dose it
prevent s.to_a.certain extent development of the vaccinia and
avian diphtero variola.viruseso
The favourable action of Na ascorbinate on virus multi,
plication suggests its eventual use in the preparation of vi-
ral antigens,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
432 ?
71.o0- ACAD.ST.SoICOLAUo k e v ,~.L zed vaccii?.:s, A new method.
for the reparation of attenuated vaccines ,_against ra-
bies. tick borne encephalitisQ,m herpes influenza and
-
ornithosiso $to cerc, inframicrobiolo, 196o, ll,, 4,
489; Arch, fo expo vet0Medo 1961, A~A 349.
Our metallized vaccines, obtained with heavy metal
ions,open up we beleive a new-road to the preparation of
attenuated antiviral vaccines.
The immunogenic activity of the antirabies vaccine pre-
pared with 1e5%lgooo sIPI.-ver nitrate is very intense, both in.
laboratory mice and rabbits. By.elimination of the excess me-
tallic ions from the metallized vaccine, an. antirabies vao-
cine was obtained more active thant that of Fermi,, and which
maintains its ectivity during at least 3 months. The virus,
in the 11 silvered 11 antirabies vaccine is submitted to an
irreversible'attenuationo _--
This-same ,vaccine, prepared with the radioactive silver
slat (Ag11oN03)"Permits study of the migration of the virus
vaccine in the organism of the animal and of the mechanism of
tissular immunization characterizing viral diseases -
The tick-borne spr simmer. enc. halltis virus in the .
brain triturate obtained from mice which died of the experi-
mental disease0 suspended in a lo5:1900. AgNO3 solution and.
maintained. 44 hours at 37?09 is transformed into an anti-en-
cephalitis_vaccine which immunizes mice against the homolo-
gous virus. The metallized anti-encephalitis vaccine is much
more efficient than the formolised tracc ine o
Comparing the immunogenic activity of the fz;esh formoli~-
sed vaccine with that of the silvered vaccine 9 stored even .120.-
days at +44?C, the evident superiority of the latter may be-seefl'
We were able to demonstrate the powerful immunizing acs' .
tion of the silvered antiherpesavaceine. This vaccine was pre-
pared wither with 1/lo cerebral substance from mice which had.-
died of experimental herpes encephalitis,, or with the fluid..of-
in vitro cultures of the herpes virus on human embryo cellsoThe
best concentration of silver salts for these vaccines wasog25s
l,ooo silver nitrateoThe silvered vaccine prepared with in
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
433
vitro cultivated virus is more active than that prepared -
from the cerebral substance of mice which died of experimen
tal herpes encephalitis,
The anti-influenza vaccine prepared from the.allantoic
fluid of embryonated egg cultures and containing o.5:1,000 sil-
ver nitrates administered by peritoneal, intramuscular, subcu-
taneous or intranasal route, immunizes mice against the fresh.
virus inoculated by intranasal route. However, the maximum ac-
tivity of the silvered anti-inflienza vaccine is obtained when
the animals are immunized by intraperitoneal route0
The silvered anti=ornithosis vaccine possesses a manifest
immunogenic potential. The 3:1,000 or 4:.lflooo silvered vaccine
prepared with lung suspension from mice which died of experi
mental ornithosis, immunizes very well the mice to which it .
was administered in 2 intramuscular injections or once by in-
tranasal route.0This vaccine, more active than those prepared.
:from virus. cultures on embryonated :eggs or from cultures
ried_dut '.according to the Shindarov method (in vivo and..then_
in.vitro), is likewise much more efficient than the formolised
vaccine.-
Just as active as the silvered antirabies vaccine is the
cered antirabies vaccine. The brain triturate, obtained
from. a rabbit which died of fixed virus rabies, suspended in
a o06:l,ooo copper nitrate Pr 0a5:1,000 copper chloride, be-
comes a very active vaccine. The o05:1,000 copper chloride an-
tirabies vaccine appears to be more active.than the o06:1,000
c.opper..ni.trate vaccine.0The latter, however, maintained, its
immunizing properties for mice a longer periad of time.
jllo. AoDEMICI, ALOPETRESCU, P.ATHARASLUo Associated anti,
toxic, antimicrobial and antiviral vaccinations .(We-
rimental.investi ations , St?cerc.inframicrobioie,
196o, 11, 4, 50-
was observed that mice immunized Jtiki.J} a c;c::. iex d.i.ph=
.It
therodtota,.nusG.poliomyelit3.sminfluenza vaccine, present comple-
te resistance to the challenge infection with the influenza
or poliomyelitis virus and in a lesser degree to inocu=
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
-434,-
lotion with diphtheria or tetanus toxin. Guinea pigs it unize'_ ..
with the associated diphtheria?tetanus=pertussis-influen za vacei-
no present complete resistance to inoculation with diphtheria
or tetanus toxin and medium antiinfiuenza HAI antibod,Y7,-~ titers,,
lower than those of guinea pigs immunized only with the anti-
influenza vaccineo
The experimental results confirm the findings of other
authors on the slight antidiphthbria and antitetanic i. uniza=>
tion of the mouse and the fact that serum antiinfluenza HAT.
antibody titers do not tally with the degree of resistance to.
virus inoculations therefore to the degree of immunity proper
Vaccination with the complex diphthero-tetano-pertussis=influ?-n-
za vaccine induces a good immunity against diphtheric and to-
tanic toxin, poliomyelitis and influenza viruses,
7120= AcDEREVICI, C0SATMARI, ALoPETRESCU, P?ATHANASIU4 I0POPA,.
CoHONDORo Associated antimicrobial and antiviral va ina-
tion to volunteers o Serologic ctk 4 idemiologic , datao
Kiorobiolo, parazitol.o, epidemiol?, 196o94,. 3230....- ....
The authors present the serologic and epidemiologic re-
sults of associated vaccination to volunteero, showing that
the best results are obtained with simultaneous administration
of TAB and antiinfluenza vaccines. The results are not so good.
when the two vaccines are administered at different 'intervals,
713oa O0MITROIU, GOBALMUS, MoANDREESCU9 M0PQPA0 Serum aldolase
modifications in rabbits with alloxanminduced diabetes.
Conference on Hepatitis, October 2123, 196o?
The investigations had in view appraisal of the,serum al
dolase test in rabbits with alloxan=induced diabetes. The re=
aalts obtained showed increased aldolasemia values, probably
due to an abnormal acumulation following destruction and hyper-
permeabilization of the cells damaged by alloxan, on the one
hand, and, on the others the fact that aldolase is not used in
the process of tissular glycolysiso
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
435
7140- AL.PETRESCU, P.sATHANASIU, S..CIRNARU. Hepatic morphologic
lesions caused by a viral agent isolated from infants..
with an acute toxic respiratory Syndromeo Interregional.
Conference on epidemic hepatitis. Sinaia, October 21-22,
196o.
A description is given of the lesions induced by this
viral agent at the level of the liver: diffuse and nodular
infiltrations with lyympho-monocytea, hepatocytophage proces,
ses, lesions of the he-atocytes, fatty charging of the proto-
plasm and pycnotic alteration up to lysis of the nuclei.
?15..- N.CAJAL9.O0BURDUCEA, S..MATEESCU, G.WINESCU, M.CE?.
PLEANU, Y.COPELOVICI0 The evolution of some experimental
viral diseases (influenza poliomyelitis, Coxsackie, ra-
bies, herpes) under the, action of radioactive phosphorus
and iodine (P 2 and IM). Acta.Virole..Ung,.196o,.2,2,181;
Stn cerc. inframicrobiolo, 1961, ..2, is 29; Excerpts
Medica 19610 Sect@IV9 14, 12, 3516.
Experiments with the influenza, Coxsackie, poliomyeli-
tis9 rabies and herpes virus, in adult white mice exposed to
the action of gamma and beta radiations emitted by p32 and
1131 showed that increase in the sensitivity of the animals
to the pathogenic action of the viruses tested is obtained
tinder the effect of these radiations:
_Under.the.action..of...internal radiations, some viruses
as_.for-.instance -poli3virus type II (L~F1) may realize the-
experimental-infection also.. after_.inoculation by routes other
than the selective one (peritoneal and subcutaneous). Moreo-
ver, the Coxsackie virus (A2), non-pathogenous for the adult
white *iuse, induces under the action of radioactive phospho-
rus and iodine a high proportion of lethal paralytic cases.
The results obtained are explained by lowering of the
animal's resistance, a true " irradiation disease ", develop-
ping due to diminution of serum properdine and cellular meta-.
bolic alterations that favour the, mechanism of viral multipli-
cation
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
436
The increase of the rad ? -9c tivity threshold in nature is-...-.
assumed to ifluence morbidity from viral, diseases and the appea-
rance of new and severe viral affectionso
716. R.PORTOCATA, I.BAMUEL, GoTIRNOVEANUe Presence of cytome- ..
.ic inclusions in the submaxillary _l ds_,of the newborn.
.cta Virol..Ung..1960, 2, 2, 188; Probleme de Morfopato-
logie.1961, Ilnd vol., 265?
In the submaxillary glands of 4 out of 32 new-born chiles..:
dren who died of various affections, the presence of intranu-.-
clear.cytomegalic inclusions was found within the hypertrophie
cells. Their incidence is similar to that observed in other
countries. Their presence points to the existence in our country
of a disease with cytomegalic inclusions, that has been detec-
ted until now only by histologic examinations?
717.c NoCAJAL, O.BURDUCEA, M?ABDREESCUo Sturm the value of
serum ro erdine under the action ofradiophosphorus..
(P32) and radioiodine (Z) St. c erc .inframicrobiol. ,
1961, l2, 3S 2970
Internal gamma and beta irradiation with P32 and 1131
brought about in the white rat an obvious decrease in serum
properdine. This decr4ase occurs 24 hours after administration
of the radioactive substances and reaches a maximum after 4
daysO ..._.._ _ _.._ ._ _-_ _..- ._-
The experiments carried out also-demonstrated the role
of properdine, in nonspecific antiviral resistanceo
718.= G.MARINESCU, Z.MARGARIT o . An adenoh eraller is form of
infectious mononucleosis. Spitalu). 1961, 280o
Infectious mononucleosis, owing to its pantropism, takes
on a great number of clinical forms. An.extremely painful cli-
nical form with adenopathy is described.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
- 437
719- G.o MARINESCU,AOMOSTRGESCU.? Ve iculo.aphthou sto~ati
Contributions to the etiologic and clinical stu .Report
read at the Society of Medical Sciences, Dedember 7,,1961,
From an epidemic focus of vesiculous stomatitis in a.day
:oursery, affecting 16 children, Coxsackie virus group B was.
isolated and identified by histopathologic and serologic in-
vestigations-
72o G0MARINESCU9 I,MICHNL On the value of the presence of
giant cells in rhinophar-Meal smears for the early ...
di nosis of measlesse, The 3rd Congress of the Czechoslo-
vakian microbiologists on respiratory viral diseases,,
May 1961,
The frequency of giant cells in 1,798 rhinopharyngeal
smears,, from l04 measles cases, was studied in various phases
of the disease-
The presence of giant cells was confirmed in 6o per cent
of the cases towards the end of the incubation period;75 per
cent in the invasion period and 58 per cent at the beginning
of the eruption; it then progressively decreases to 9,4 per
cent i.n the 4th day of the rash, with significantly altered
cells, Giant. cells were found in a single case among the con--
?trol group (33 healthy children or children with various ca-
,~?rhal rhinopharyngeal affections) o
72i - OoMITROIU, .C,BARBU, M0POPA, O.-SFERDEANU- Etiological
and'serological investigations in the course of some.,
affectionsaaccopanie d.by rashes in children,, St9 care*
inf ramicrobiol Q' ,1961,. 12, suppl o , 2719 Rev-ScUMed.
1961, 6., 1?2, 650
Etiological and serological investigations were carried
out in 7 cases of atypical morbilliform eruptive fever, &t-
tempts at virus isolation are.negative :: iUr, 3 passages,
Serological findings, however, reveal an increase of comple-.
ment=fixing adenovirus antibodieso These increases being evi-
dence of.a recent infection,, they point to an adenoviral
etiology,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
-.438 -
722o... G,MARINESCU, V,MABES. e., ,orphopathology of respirator
viral diseases.; Symposium on respiratory viral diseases$
Baia Mare, September 29-30, 19610
The authors describe the morphopathologie aspects of all
viral diseases involving the reapiratory.tract, stressing both
the points in common and the differences,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
XI
Monogr__aph?
1 v 18
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
440
1,? Recent Soviet investigations in the field of influenza.
Ed,AcadLR.0P0R0, 1950, oL pages 5 tab., 25 bibliographic
indications,
A monograph reviewing 25 publications by. Soviet authors
in the field of influenza published up to 1950,
The bibliographic material is presented at the end of _
each chapter concerning" Im Etiology, pathogeny, experimental
study, immunology and enidemioiog:v of influenza; II, The cli.
nice of this affection; III* diagnostic methods; IVs prophylaxis
and treatments
20o Recent ? oviet investigations in the field. of encepnelitia
dee0 Ed?AcadjR,p,R0 1950, 78 page, 4 tab0, 20 bibiiograa
phic indications.
- A.monograph regarding speciality Soviet literature.up
to 1950. A review is made of data concerning louping Sian encephalitis, Bielorussian encephalitis, Farms astern erg
cephalitis, acute human encephalomyeliti8g Dialangar encepha-
litis and equine encephalomyelitis in the U.S.S.R.
In the chapter " Experimental studies on the problem of
encephalitides " mention is made of the Soviet author R,M?.__
Schoen, who cultivated different encephalitis viruses in tumors
The work of this author regarding the latent forms of St.Louis
encephalitis is likewise cited,
ACADe8to8,NICOLAU01.Inframicrobial hepatitid?so E ado
ROPOR,, 1954, 76 Pga., 22plates, 14o referenceso
- -- -. u -The . author, . on scientific bases , synthetises his own
original conception on. the plurality of human hepatitides and
hepatitis viruses a His modern conception is founded on the
biologic transformation in naturef microbes and inframicro=
best under the influence of new eflvjronjaental conditions arib
sing due to the intervention of various factors, Thus, star
ting from the classical "epidemic icterus ", identified as
" Botkin's hepatitis q due to virus At which caused the great
epidemics during the last two wars, the authors identifies
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
~.4 fl
00- All
It the sclerogenous hepatitis " due to virus C.and the " hepati-
tis due to the non-hemagglutinating D virus "o The natural pro-
cess of transformation of viruses cannot be considered as ended
a3od it is still to be found in full progress, Next to the above
hepatitides, the author mentions the variant due to virus B
homologous serum hepatitis " or the " Sergheev and Tareev di-
sease", casually transmitted by contaminated serum or plasma.
The character. of each of these types of hepatitis are analysed
on the basis of the research -work of the Rumanian school of..
inframicrobiology and the data published in modern medical li-
teraturee
Chapter I, " Botkin's disease chapter II " scleroge-
:?:()us inframicrobial hepatitis " and chapter III It homologous
-::.,rum hepatitis ", give a brief description of the clinical and
epidemiologic aspects, the results of recent investigations on
the etiologic agent and the characteristics sf the histopatho-
logic lesions of each of these hepatitides.
The last chapter deals with other human and animal hepa-
titis viruses... _- .
__.To conclude, the entire problem of inframicrobial hepati-
tides, as it appears-in the present stage of our knowledge, is
sunned.up_in..a table. This synopsis represents the results of
the.-investigations carried out over more than 18 years by the
Rumanian. school of inframicrobiology.
4a- V.BABI'S, " Selected Works.!'. (published by-an ec}.iting.boaid;
acad-~St S,DNicolau,..M.Babe$, F10Horodniceanu) Bd. Acad.R.P.R0
1954, volols 64o page,
LOB4,BISO Bibliographer of the works of Victor Babes . Rd.Acad.
R.P.RO, 1954, 88 page
The present work is a systematic classification of Victor
Baber's 1300 publications, compiled according to the year of
publication and subject matter in order to aid investigations
in connection with the works of the Rumanian scientist,-
An and chapter gives the, most important bibliographic data
in the life of Victor Baber.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
?r4.2-
6W=-ACAD0ST,oSJICOLAU, Cancer, ?d virusesi.-''r;,?.e considera?
tions on the viral hypothesis of cee Ed,Acad0R,P0R0
19559 79 pago, 22 plates, 89 references,
New data are reported on the pathogenesis of tumoral
processes under the influence of inframicrobial agents, len-
ding support to the viral etiology of cancer,
The material presented is a synthesis of numerous per-
sonal investigations of the author on inframicrobial morpho-
logy and of the findings in this.field of investigatioA.;, pu-
blished in literature up to date.
In a brief introduction the author shows that the star-
ting point of his investigations was the presence in sclero-
genous hepatitis of pseudo giant cells similar,to those pre-
viously described in many other viral diseases,
In the chapter on " Cellular proliferation under the
influence of inframicrobial germs " the author reviews 23
viral diseases and describesbitotic cellular proliferation
brought about in the organism the activity of inframicrobial
germs,
In the latter part of the volume,, the phenomenon obser-
ved is discussed and interpreted, The author believes that
this property of inducing proliferation ("proliferigen.ous
property) in the.affected tissue cells, is almost general
with all viruses, since it results from their metabolic re-
quirements,-viruses growing only in live cells. The younger-
the calls-and-the more-active their.gultiplication, the more
favourable -they are for virus growths
A Russian and Frenc-. translation of the Rumanian text
completes the volume,
7p? A0DEREVICIo=Prim viral meningitis. Edo Led, Bucuregti
19559 131 r-,, a9 ;rigs. IX tabs,
After a brief history of primary meningitides and an
attempt at drawing up- a systcmatic.list of the diseases which
can be classed among these viroses, the author describes the
various clinical forms. and possibilities of making a labora-
tory diagnosis,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 : CIA-RDP80T00246AO19200640001-8
glassification ,according to the etiologia agent' is
proposed,
The ,following chapters discuss the properties 'of vari
ous virus strains .of primary meningitis, followed by a wide'
description.of the morpho"pathology 'and immunity, completed'
A. description is given of experimental investigations'
on animals according, to, thee data "of various authors and. ' to
personal reSearCho,
paramount 'importance `for the'.cointrol.`of, this.viral. disease o;
reveals ' the role of murne vectors, whose destruction is.,o
The epidemiologi& data obtained in,.personal. research,,
with data. refering to attempts made 'at `vacdination'o
The book comprises -lo chapters?.
80- ACAD.STgS,oNICOLAU. Elements of .general. inftamicrobjologgo
Dd0Acad0R0P0R0 ' `19956 4.. ' 2u plates
? x 77 ~.~ 5 ,Apfl5p
After a "brief introduction into the 'study of inframi-.
crobiology, the 'author "outlines' this science, and stresses, its
paramount importance inhuman, animal and plant pathology; -.
He theh syetem 'atieally analysis "oh,the'?basis.; of 'a rich docu
mentary 'mate rl?al the 'genesis' of 'these=''micro"organisms and_.-..,
their propert4.es `studied-`bj t" most' up=to?date_~.meens of; ....; ' -
iawestigationm'
"A 'description 18.. given' 'of ' ..their : itorphology `established
by.- various ? optical -means ,from the simple to the...-electron
mi 1roscope-, . of their 'structure and' chemical-.Land ;biological
In order to illustrate more clearly .the.'particular ? be- ,-
haviour'of''these germ' -in'-the human',- aniaaal-or plant organism
the author describes several exper mental9 histopatha 0gfc
and...morphologie techniquesg: commonly. applied :.in.inframicro-
biology, asvell culture of viruses outside the organism, in.
embryonated eggs, tissue cultures and tumours,. a method. ini-
tinted by-the author together with professor Cmhevaditie ..
.. -.In. chapter VI9 concerning the behaviour ..of inframios e .
bee in the organism, acada8t?gicolau.describes the affinity
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 : CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
of viruses for certain tissues, which factually implies finding
of the most favourable living cvaaitions for the parasite gcrMo
A description is given of the spread of viruses within the c -
ganism, the mechanism of viral infection, resolution of the
conflict between inframicroorganism and the organism, the in-
teresting results of this conflict both for the organism and
for the biology of the respective germ. Thus can be explained
the spontaneous transformations suffered by the infraicrobe
under natural or experimentally controlled conditionsa
Studying the consequences, for the organism, of viral
infection, the author describes the alterations that is place
in the.cells and tissues under the action of various crobes, laying particular stress on cellular inclusions an,4
their sigpificance, accepted today by all research workerso
boa AC,~D:STQSoNICOIAU? Inframicrobig infeatious,hepatitides.
!d.Acad? R.P.R. 19579 73 pago a 188 references,
The author studies in detail the k human viral infec-
tious hepatitis s6rinogwhich he designates as different noso-~-
logic entities; Botkin?s hepatitis (viral epidemic hepatitis),
sclerogenous-hepatitis, nonhemagglutinating.virus hepatitis
and::,}somologous serum hepatitis (serum hepatitis or Sergheev
-.._._._. -.__._. ..__,__._..-. ...~ ..._ . r_
Tapeev?s disease) c,
Arthropod-borne human and. animal hepatitides and animal
hepatitis are likewise reviewedo-.-
_. _-k On-the-basis-of-the 15 years investigations. of the . Rumacm
nian-Sehool. of-..Inframicrobiology-concerning viral epidemic.-w?~
hepatitidees-briefly..related- in the work, -the author.-sustains
the - plurality--of -viral :hepatitides, - iaosologie ,. entities.,that
di f,fer_fr4*-the.-eti.glogie view point, and draws up a provisio-'
nal systematization,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
lo. = N..QAJALe - iaborator ? diagnosis of human viral diseases,,
Edo Acad?R.0P.R., 19589 585 pages, 117 figs-
The first part comprises nine chapters in which are ex=-
poundedflon.the basis of data in literature and the author's
experience, the general methods aDDlied in the diagnosis of via
ral diseases (the utility of virologic laborator..y, collecting-
and and transport of infectious material, techniques for the.isolam
:ion and identification of viruses in laboratory animals, QM-..
ryonated eggs and cell cultures, storage of viruse techniques;,
for microscopic examination of viruses and specific h1stopa -.
thologic alterations,.serologic tests, cross immunity, anaphy-
lactic reaction etc.), and in the second part (7 chapters)
diagnostic possibilities in the-.most important human. viral di-
ceases (eruptive. viral diseases, neuroviral diseases, pulmona-.
r-%T viral diseaseq,lymphoviral diseases, epidemic hepatitis, ve-
nereal and ocular viral diseases).
Numerous original techniques devised. in the Institute
of Inframicrobiology c. the R.P.R. Academy, are described,--
and in each chapter a series of diagnosis techniques, listed.
according to their importance, synopsis and partial and gene-
ral conclusions are given.
Next to a-rich bibliography there are also 117 drawings
and original photos, which suggestively complete the text,,
110- AoDEREVICI. " Influenza various aspects. of the . problem,a .
Biblioteca Medical ~ X., Bd?AcadoR.P.Re 1958,472 p.9 156figs0
This volume ia..divided into three parts:
1. Data on the micro-organism.
2. Interrelations between the host cells and the
virus.
3e Data on the macro-organic&o
The first part of the book gives data on the classifica-
tion,_chenical composition and biologic structure of the in-
fluenza virus, data on toxigenesis, spontaneous and directed
variahility, as well as multiplication in syanergie and anta-
gonistic conditionse
In the chapters of the second part, the relations
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
446
between the macro- an,.t micro-organism are established, descri-
bing the elements that stand at- uae basis of tae .reactivity of
the body and the component elements of this reactivity, stres-
sing its nervous histo-physiologic substrate
The importance of IoP?Pavlovgs nervist conception is
emphasized.
In the third part9 the clinical forms are des
cribed.; stress is laid on the histopathologic modifications@
determined by toxic properties of the influenza virus and the
toxi-allergic character of influenzao
The mechanism of the es t,ablishment of immunity is inves-
tigated, mention being made of the various factors which in-
tervene in inwiunogenesiso
A detailed analysis of the various types of vaccines and
their application is given in the chapter on anti-influenza .
prophylaxis; the probleme of nonspecific prophylaxis is like-
wise treated in details
. _. -_ This , work is a synthesis of the anti-influenza campaign
in the R.P.Ro 9 headed. by the staff of the Institute of Inframi.-
crobiology of the R.oP?R? Academy, in collaboration with th$
Ministry of Health and. Social Welfare, the sanitary units in
the country and especially.with the Sanitary Anti-epidemic net-
work in the towns of Jassy, Bucharest, Galatzi, Brasov, Cluje
Tgo Murego Timicoara and Bacauo
The results obtained in small groups of persons vaccina-
ted with a -vaccine prepared in the Institute of,Inframicrobio-
logy demonstrated the value of mass prophylaxiso
Several proposals are made at the end of the.monograph,
for the organization of anti-influenza prophylaxiso
12 19GHEm2GHIU 9 S.MIHAITA 9 V 0TOMESC1f, . 10MARINESC'J,P 0ONCICIU 9
NOPOPA0 Swine lagueo Edo Acad?R0P0Ro, 19589 299 pages,
28 figs 8 tables, 516 references.
This monograph is a comprehensive study of swine plague,
The first chapter deals with the biology of the pathogenic
agent9 stbessing its relationship with other viruses or associa-
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 CIA-RDP80T00246AO19200640001-8
ted micrubial flora, its multiplication and sensitivity to
physical, chemical and biologic agents, An important part df-.
this chapter is dedicated to controlled transformation of the
characters of the swine plague virus by means of its cultiva-
tion in other animal species. The chapters on the clinical.,._.,.._:
anatomopathologic and diagnostic study of the disease are.rich-
ly illustrated by the personal observations of the authors.-
The illustrations accompanying these chapters show the_-clini
cal evolution of the disease, which is often polymoa; p iO and
the anatomopathologic modifications resulting from the action
of the virus or that of the virus associated to secondary.germs0
The study of i,:munity and specific prophylaxis is wide-
ly developped and contains many details on the preparation,....,
_.Zoperties and use of the biologic anti-swine plague products.
The chapter on general prophylaxis discussed the epizoo-
tologic viewpoint and gives helpfull indic,,.pons for the pre
vention and control of this virus disease, particularly in the
breeding conditions in ,uc'
13?? V0BA$ES, " Selected works " (Published by an editing board:
acad.St,Sjicolau, M,.Babe-5, P16Eorodnicea:nu) Ed.Acad.R.P.R09
vol.II, 1959, 896 pages.
1. . A.GRINTESCU, C,,IACQ$.. Diseases. transmissible from animal
1_0,0 Ed, Acad.R?P.R., 1959, 489 pass,, 85 figs., 4
plates,
A monographic study on specific animal diseases to, whi. c:k
man is also receptive, diseases currently named zoonoses. Be-
sides several viral and bacterial diseases listed under this__
.
designation, all animal diseases of viral and bacterial.origin'
as well as mycoses acid parasitoses that tkireaten human communi-
ties, are dealt with.....
The subject natter :i.s divided according to the causal_..._...
agent, and in the introduction general considerations are made..
on the role of natural foci, on the common sources of infection
and infestation, on epizootologic and epidemiologic characte-
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
risties, as well as on the principles that;; stand at the
basis of the prophylaxis of these diseaseso
15.- G.MARINESCU. Acute infectious lymphocytosis and infecti-
ous mononucleosiso Ed0Med0 196o, 309 pag., 4o fig.
New data are brought concerning these two diseases _.
little known as yet in our country. They have been described.
together in i.ew of the marked tropism of their causal agents,
probably viruses, for the reticulo-endothelial and lymphatic
systems throughout the whole organism. This is mentioned in..
a brief introduction,after which each of these affections is
dealt with in detail0
Each chapter is of a corresponding didactic and scien-
tific length, stressing in acute infectious lymphocytosis,
th@etiopathogeny which is still under discussion and hemato-
1oRic alterations, that play a principal r9le in establishing
the diagnosis. The chapter on infectious mononucleosis deals
9 .> .a ..,Y .1
especially with the maltipld. 'linical forms and the possibi-
lities of an early diagnosis,
The book is illustrated by many figures and a rich bi-
bliography (2000 references), comprising the original works
of the authors the contribution of Rumanian investigators
and specialty pubilc ations in the last lo to 15 years.
160- V0TOMESQU,.I0GIiEORGHIU,. I0ISOPE$CU0 Foot,and mouth disea
se"0 Ed.ACad0 R0P0R0, 196o, 409, pagos 85"fig0
The book presents the newest data in world literature,.-
the contribution of Rumanian investigators and of the authors
to the main problems that stand at the basis of our knowledge
and the control of this terrible diseased
The following chapters come after the introduction and.
the history: Study.of the etiologic agent, Receptivity.-.-Exile,
rimental infections Pathogenesis, Epizootology, Clinical stu=
dy, Anatomo-pathologic study, Diagnosis, Prognosis, Immunity_
and Prophylaxis. Particular attention is paid to the physical
and chemical properties of the virus, citing data from world
literature and especially the works of the Rumanian scien.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
tists Levaditi and Nicolauo
Study of the plurality and variability of the foot and
mouth disease .virus is founded upon the initial works of--- `
Vall6e, Carr4, Waldmann and Trautwein and the new publications
of the Soviet authors Kindiakov? 13aiadinove etco
Description of virus type determinations begin with the
method introduced by the Rumanian investigator Prof 0 of o sI CA
in 1929, complement -fixation and the modern methods s
neutrali,A?ti nn In tissue cultureso
authors discuss in detail
In specific prophylaxis* according to various me-
thods, the preparation of sera and vaccineCOntributior to the prepa~
and likewise scow their own
,ation of the vaccine done for the first time in our co ntry
,:nd which led to eradication of the 1959-196o . pi t o
VOBABESO 19 Selected work s , B ,, voloIll (under the.case of,
an editorial boat~d? 0 AcadhS'oNicalaug t,BabespC,Uari.nes-
cu) EdoAcad?RoPoRo "196l9 298 pago
18m- ACADoS ACAD0AL' RADULESCU$ N'oe',,C0IISTA1jThlS-
''dSo~ICO~A~~
CU, R,CA3AT,s QxoMAgIWSCU.Polior i?s Ed0AcadoRoPoRo
19619 ??1 page, 40o figso -?
PO~,Tp~: LITIS is written at a high scientific levels
under exceile.nt editorial condition,sq and presents the latest
data concerning one themost important and actual problems-in
di-
m ,decineo The twelve chapte)ra of the book deal vitastct sd.i
to date with all the p
sease completely and are up
this affections which has preoccupied invest:i_f:ators for al-
most two centurieso
Chapter I gives general indications on the etiolocy,
Chapter II shows the.
immunity and prophylaxis of the disease
epid.em.i.ologic aspect.., pointing to the main epideniiol0gic fac-
tors, the contagiousn:5s of the disease, its mode
tion and sources of infections and particularly its evolution
of the causal
in our country. Chapter III is a detailed study
agent and deals with its morphology and chemical constitution
as well as with the reaction of the vh~uvzrus various
especiall.y in
the
and physical ag-ents n cultivation of
tissue culture,study of different virus st:ra.ins~ ei
Chapter IV reviews numerous Investigations regarding
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 -
_experimental reproduction of the disease in more;e mur,~
nes and other animal species. . apter V relates the invest
gatioms linked to the physiopathology of poliomyelitis.'
and
ckapter' is concerned with the nonspecific factors that
influence the clinical evolution of the disease. In chapter.
VII there is a detailed study of the lesions produced by po-
lioviruses in different organso
Chapter VIII presents the most up to date investiga-
tions and the newest methods for isolation of the virus, pre-
paration of type immune sera, virus i dentificationo In Chap,
ter IX the authors deal with problems of antipolio immunitye
both active and passives paraimmunity and interference in.
poliomyelitis, data of exceptional interest from the practi-
cal standpo i.nt o
Chapter Z comprises specific prophylaxis by means of
antipoliomyelitis vaccinations Chapter XI describes in detail
the symptomatology of the disease in all its phases of evolu-
tion, the clinical formsg.respiratory imbalance syndrome: in.
the course of the, disease, complications' positive and diffe-
rential. diagnosis, prognosis and methods of treatmento ft-_
nallye Chapter XII is an ample study of the period of seque-
laeo
POLIOuYNLITIS is also a review of specialty scientific.
literature from abroad and that of the Rumanian investigators
and olini:,i,anso The exceptional contribution of the Soviet
School-in-solving the different aspects of poliomyelitis--and
particularly. the sucoes obtained by Soviet scientists with
the antipolio vaccine are also described,
POIRIMELITIS is useful both for specialists, in this
problem and for doctors specialized in wt;Ler fieldso
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
!4,52
BA x7. G o = -7 75-9 63.9 95
1. 9 14488 o X52.9.
16.6. 225-233.
6949 X9897;
4360 476 9 477 9 4799
7139
BARBU Co- 1S0? 4949 7219
BARBULHESCU.GE.o= 3459
BENEDICT. I312 .
BERCAN A 5299 570, 5739 63o
BERCOVICI 00- 163.
BERLOGEA 10= o69.613 0.642
6848 68,E 686
BIBERI-MOROI .So0 1599 1629 175
BIICU NO. 161
BIRZU I 93.
BIRCA Ao? 1969 1980 199, 2o3,
BIRCH No- 1329 149 142 9 1539
BLI DU Po-.
BOCIRNEQ. C o -a
BOERU V o-
BOIU Ao'.
BOTAN A0
?
BOT.EA Go=
BOTEZ Mom
BOTEZ Qob
BRAN LO
2769 27.79 2819 2829
3.030 3o4s 3?59 3o7
1429
2o4.
.30.
1459
446,
397
399
661.
245.
1589 1629 1630 1736 178,_
2889 2899 29o, 297929893009
419 47, .128, . 0 1369 1370 .1389 -- 139,0143.0. v.
1549 156t, 1579 1659 1699 2360 3014 44'4'9 '59
4480 4589 4679 4699 4739 474, 4759 492
1129 124
1.91
548.
BRAUNER R0='..
BRONI'i' Alm- 348 9
379,
3989
4269
4639
700.
BRUCKBER. 8 o- 121
BIUCUR No- .. 608
6o8.
3499 350, 353, 3569 3589 3629 3639 .383780
3809 3819 3829 3839 385 0 386g. . 389 ,, 396.9 397 s
4oo, 4log 411 a 413 0 414S
419.94269 424,425 9
42.79 4309 4339 4409 446,449,457,459.,4619...
46494769 9 4799 4909 . 9' 6820 68396959
BURDUCEA O o- 35 9 2o2 9 479. 9 715 9 717
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
.
ALBOIU 5299j
126, 128, 129,146, 71 g 39o, .196, .1984 19 .,2o1,
.5 9 257,261,267,328?
0_.g4
202920.3, , 2410
38484, 492,6 929.4
ADERCA I.- . 28 9 32 .a ? 9 34o . 369, 3704790.9 9291160 117,
AYI" a .TIC_ I C U
A
ABRAHAM Ao c 653
ALBU C`o~
9, 10501139
515,516,682
ALBU ToT. 523, 526, 529, 53,01x,331, 533, 5689 5709571
A7.SDEA M = 449, .
AI,ERANDRESC1' .Ram .614, 615
ANAGNOSTE Bo=. 62,,, 67, 68, 699 70-1)-72,)7.39,7-69 78, . 8o, .81,83 ?
84 .859 194 2919,2949296o 3649 3659.366,367,
368, 3699 370, 387, 403, .455o .6129621,625,626, .
628, 629, 6309 631,632, 6339 637, 638, 639,64o,
64.1 m ? . , * __ .. , --. . , ~, ;.
ANDREESCU M,- 40, 147t 1509 168, 17191799295, 470, 0297138
,717,.
ANGELESCU Ho- 594
ANGELESCU.Mo= 299
ARMASU V,m 219, 437
ATHANASIU p 210-1129 1 ,. - I. , 168, , .17S,2o2,2o4,222,
2269 25 59 02619.26 .266.,.2809 292, :.
375, 386,. 3 ,. 4079. 418,42o, 429, 4416 4429
BABA Co-
o=
4.53, 4549. .466, 7?'.., .48o, .497, 4.989Z?oj;
4,539
5 ?3 550 .626,, 630, 6319 632, 633, 634,653,
7119 7129 714
B
136, 17 , 138, 139,1409147, 1519 154,156,1659
1.69, 170,2029.232 9 .702. .
BABES Mom ..48.9-250t , 6899 EL69
BACALOGLU. Do-6141.0
BALAIAN M0Soa223,
BALM A0= 684, 685
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part- Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
BUSILk V om 60, 6.._.,,14, 6159 624
BUTTE V o?' 684 g
CAJAL No-
CIORCITINA U q~
CIOCITLTEU V ?m
CIUCUty,CCU - A??
24? 2 , 3 ?37
12, 17,
59o 6k,86 g 87, 88 ....~' ,
104 105, ? 1088 .107, ? 108,9 lo"
1??'1?x29 _....,,' 1249 1269127+
e --... 122 9
1c)
111 ? 113, 1149 115, 1]L8, 1+
9 1140, 14.5014
L3 8 1:19
128, 12 1 9. E 136, +
1.54 156,1.59,160,1 175,17,
, ? 184 186-19 ?. 9.~ .l.Z6 a ?'.
1809 _' ?-
177). 179 s_...-.-- 2249 225,j
?.
201, 229 20.. 212 214 21 +
234 2390 240 244
233 ,_
22 ~
22?~ 22 0 910-
g2 0 2 28o a 28 6 1 298,
247, ?16,,"
8, 326 3" , .3479 3521 392{
3?
303'. 330 5 5 e .~ 88 899 .644, 6450-656
393,!' .44.39 5o6, 5349 ON
672 674t.6759 6779 678, 6799
661, 6689670v:-6719
68o, 69019 0ALWOV10'( S 1919- ~.. 212, 236, 422, 4719
CAPRARU RO. 92 9 93' 1o4?e 110, 111, 135t
CARANIAN vqm 574.
CABA11aAN - zo- 593,
CARP No- .- 505t 571
CARUNTU Fm-743. 114 124,_?
CEPLEAI+7~J m 2 , 35 9.86 86, 104+105, 109, 1.13,
149 201, 202, 239,2599 298, 3o7, 3159 326,
135, 9 .
3529 4229 471, 5479 6o4, 715+
CHEPTEA Ao' ' 269, 270, 2749 275
CTrSTi TI1 HLTAI.o? 3o1, 3369 621, 637
OBA"-850't) VIO- 202, 220 , 4899 493 9
CIURBSU V9?
CIRNARU 8w?
COPPLER 11 0?' -
COJOCARU Vo'
COIPANET V ?~
590.
5919 592-
594
185, 1.88; 189,
37:e 4149 4409
14 p X2.166
3 , 370
168
366.
44+ 498 9 714
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
CODREA Ao? 345 .. .
CONSTANTIN.ESC 3 A o 49
4' . U
CONSTANTIN:r,,~.`; 577 660
CONST,ANTINFSCU ~; @ c 329
162f, 173,
178, 2~1.. _. ?
24.8
j g4
,251, .2529.2536254,
257 26
CONSTANTINESCUo?. 4o4.
CONSTANTINESCU Nom 12 ]&1-
265i 2
.
o20.30;r 304.0 3030 3929
651.6.
2, 281
' 28 -
8- i2 .2~ 8 , 28 x_297,
COPEEI.,OVICI Yo= 24 26T
3
CORTEZ Rom
COSTACHEL Qom
COTARCEA Io-
t ANDACHE Do=
COOS3'
COVACI oas
CRETESCU A0=,.
CUC IiT@EANU J ??
CI] SEA . V
CURE Co-
CURTEANU Go.
g g
5, 949 110, 111 114, 126,135, 1 .,t 149 2020 212, 214, 215. 217, 219,220,
235, 242, 32 ~' 419 422
_ , 437j,4380
46-20 4669 482 48
9s 4930 496, p ?15p
331 .... .
Is ZI-1 82
4.64 ,
168, X809 494, Soo
372
695
4o2
627
299-o5o1., . 5o2., . 5446 5559
5639 564P 565,566, 567
3459 354, 4o5,
56o, 561,562,
DAMAN. Mo~ 22o.
DAN Ho. 655
D A F T p . 577
DANESCUopOPESCU Go see POPESCU_DANFSCU Ge _.
DANIELESCU Goy
7a .4
-7 117,. 3259 128, .150, 176.9.._198'
1999 2o2, 2o3, 2610 26709 483
DARINGA Ao o a 590
D.AVI ESCU 2o
DICI Ao1= 5 _ .
1? 3. o ? 6.~.~.g . 30.g ,fie-- 66, -~?? 3_?~a. 100
311, 3120 9 9 d9 3169 34 934.
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
3449
3 4,
2'8_.9
33~~ -3 1 ?, ~
? ? 3
,
6o i .361P, M2j 36,L
.
; 3?8 3 638? o ; .
2-14. 82.t
3 k 3?,
395; 396o 32 Zo; 39
.
406, 408, 411, 413,
4239 424 k
42?,f 428,.~.'~?, .?9
.9.39461
463,. 464,4 b4779.478 . ,e4-_-488
194919
5949 6660
6,
-
673. 6816
.
6829683,
7, 6889
05
9
00
7 ,
DIAOONU J'o-
495
.
DIACONU So-
DINCU.LSCU P ?-
DINU I
2oo
583.
1459
1579 2oo.
DINU R.?
102,
112i 2d , 124. 131,
1829
2269 23o9
26 0 o .
264, 314, 3159 3 0
5o9, 51o.511..
DINULESCU Go-
DOBRESCU G$o-'
DOHOTARU V0..
512.
508.
500-
595*
513, 5149, 311r.
509, 5109 5119
576, 577,: 578.__
5169
,
652
517
DRAGANESCU No-
18,.21,, 25, ,184, 185, 1889 189, 191-9211'
21319
j?1',
3309
,13-2-.
2839 2840
32o9 3219
3319 122-9
, 350,
293,
322,
39
353,
294,
32.39
334,
3559
29.5+ 296t3o1,.
324 3 3290
335, 3369.337,
358, 361,33
3649
365 o. 3669
3699
3709
376,38o 0.388.0
3 9 42?.,..434, ?
4359
439, .455., .456, .473,
499,
9 .,,6sL 6ol .6o4,
6o3,6o6,
6o7,
6o8, 610.635, 6369 7o7.
: RAGANESCU SP o?
1 1.
98-3-9 3L
219
2 3
9 . 3, e
4
652
DRAGATOIU V -
155.
DRAGOMIR Co-
249,
251, 2529
253,
2549
2659
59 e593
DRAGOMIRESOU Do-
1o2 .
DUCA E9-
257,
265, 2699
5909
5939
651
DUCA No-
257,
2629 2659
2699
593s
651
DUCA Po-
DUMA Do?.
627 9
638, 639
DUMAS NO-
om
6o3
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
DUMITRESCU Aw- 538
?DUMITRESCU 1 565.
DUMITRESCU STe- 2o,.39,.40,128, 129, 2o8, 2119,2139 221,409,
446, 46,7, 4680 475, 4.87, 579 622.
DUMITRIU.Eac 501, 5o4., 56o, 5619 562, 563, -5 566,567,
DUMITRIUUCAROL Eo- 299, 564
DUVAN X.e- 134
3D.U`;S- 505
da: 372
ETEFTERE8CU Ao=-^ 306, 5o4.
ENACHE Aom
ENACH .Td-
584.56.9 7o6,
ENE Amy . . 453
ERACLIDE M0= ..594
EUSTAFIEVICI C0?577
5289 532, 577, 584, 585, 586
FACON Eo~.?
FITARAU Ao~-
FLECHNER Tom
FLORIAN $o?
FOARTA NQ
FRADIS Aom.
FRENKEL Mo-
FREUD S~
609
411
689
Lol
362.
137, 154
163
FRIDMAN 10? 1859 1889 189, 2o8o 209,
FUHRER-ANAGNOSTE Be- see ANAGNOSTE Be
G.
6469 647, 648
GAME-GHIA Ro- 357
GARIBALDI Ao- 155, 701
x. jESCU To- 435
GHEORGHIU I,,- 519, 520, 521, 522, 523, 525, 526,. 8,x
6,x,532, 9 568, 571, 577 -- ~.
GHEORGHIU Me- 1329-1410 142, " ^ -
GHERASIMESCU 191
GHETU..Vom__.- 194
GHITEA Do- 345
GIROUD P.- 6 5gZ a 602.E 6o3_
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
457
196, 1.980 199, 2019 2o2, 203, 238. 2-219 2439
245, 246 9 328, ? 484
5779,578-
659.719 82.
626, 6319 632
399?
GRUTA M.- 91, 2o2, 217A L-1 2j, _? 3o9, 31o, 311, 312-o., 311,
3159 316, 343. 349, 356. 3629 363, .418, x?199 o
2,438, 462, 482, 5069 543, 681, 682,6839
GIRD E.- 294, 296
GROZA M 22o
HUNG TAO.- 2440 599, 611, 612, 616, 617, 6189 621, 622,
.loo .
GRUMAZk;SCU no-1320 153. 158, 162
GUNA 80- 389
HARAGEA $.- 474
HONDOR CO-.- 9... 412, 712
HORNETZ THo?? 182
HoRTOFAN D,- 101.
HURDUC G.- 399
229
. . I
.
IAOOB Co- .2600 266.0 272, 5159 5169
IAWMITEANU M0-171, 179, ..
IADICON SCU Y?- Z89-329-3.39 349,3-.64--M& 479 559 1469 17691906
IFTIMOVICI Ro-
ILIE B 0- .
ILIESCU.C0-
IOAN E
IONASESCU.Go-
IONESCU Do-
IONESCU MI-.
IOIgESCU N-10
IONESCU,Y'"
ISAIA G'oem
ISBASESCU AQ-
ISBASESCU C o-
ISOPESCU 10-
343, 682
;S89'- 370
39 40 9.51,_284, . 293 1 .2940.. 295, 29r o., 3o 1 J3o9 ,
X46, 4679 4689 473 4.95-, 4879 579, 6o4,6o6,
622
2414
476, 488, 489
155, 7o1
516, 51.7
3o6, 524
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
ISRAEL Mo?. 350
ISTRATI Me- 485.
IVAN I o? . . X22 0 :3?
IVAPOL Mo= 2oo
;:SZAA So- 696
JEVANDROVA 1101 o- 223
KAHANE G 681
KAH.A NE R . 91
EAUF : So- 161
SEIDE v,L . 44o5
KOS 10- ..662
REINDI R AoC,227.
KRESTESL Ro- 6290 63o
L
TACK Hoa .4000 5949 6o8
IAAZARESQU 0x0'181
LECCA Go= 219. . ..
LEIBOVICI *~o-1320 1410 1539 158
LEONESCU L = 1360 1379.139 912
LISSIFVIQI=OPRESC!U EL,= see Oprescu.Elena
LITMAN AD- 400.
LITM` N 8 138,. 139.0. 148 0 1519 152 9 156, 16,, 169 s
I,IUUCHUNG=P&I0-14? 218,
IAZINSCKI. 5o4
LUPASCU Low 578
U
WAIITEI E .632
RICATIDE ELo-
. IA1ICATID. T 0=453
IOLIU.No? 37.
TTA le- 1329 1589
MAIRG, a oa . . 493
MARCY;LESCU -1, - 50.
NARUARE. 10d 634
VIUMS V 4999 722
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
718
MARGARI`[' z0CQ'
.
MARINESCU QrH. = 35 0 121 .1249-1349.1 9.181,9-18 0 188, 1.89 9
e 19 a . got 2029 2o48 206 9.
D2 4
2184 221, 2229..2?-39:P-319244- , 3159 33
34 69..3 ,..3570 22O
ii; 88
4 41 9 0 0 451, 452.0.481.,489.s
0 4960 5169 517, 5889 591fl 592 596 597k
6o2, 6o3, 611,0 613, 6160 6179 .635, . 0 .64:39
65o, 6529 6 9 6550 X60 .659,,.66o., 661j
662* 66L 694- 6979 694-2019 715 9 713,2
Z& :b. 9
i 1 ' U SCU 1 , 7 . 5259 526
MARINESCU. SP.w? '5'919:-.592 . ova
MARINOV L o??, M 519 9 52?0 .. 52~K9. 52.2
MARIA M 627 9 638 9 q _ loo l04
UATEESCU So- 12 1- , 359 8 93..,0 959 980 999 .0. ,
k
1o6 9 log s 110 , 1110 114, 123 9 135 9 182 0 2121
214, 2150 23o, 2414, 2560 259. 2689 421, 67o,
6730 692.9 6939 715
320E Do- . 4o5
ZTERCVICI Ao-. 200.
' SROBEOU limo 115 0 145,
i ICHEL I. 374, 72o
CLOSI.E.= 345.
CU I , = ? 142* 1739 288
2` RAIL AL.= .. 132, 1789
..-U AILEANU .1, 128-
531.0 532 0
2INCULESCU
P.:ERSAN o'' .
9
53 . 5359 5 ~0 ''539fl 'S z0 0569 0
!05.9 4jLq, 66?-
453
MTRICA Mo?
115,
145
IITROIU 00?
134,
148'152, 1 619
1659
166,
a
1.8o,2229
436;
4379 4389 4669
4949
5099
5100
51295139
514
5159 5169 517 0
652o
9
721
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
29o, 3030 3o4, 3o7
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
466 ~. - .
MIRZA?EMINFT Lv- 1.09-119-149 22 ,. 299 54-f56 .263,. _,, ,27,11.
MORARU A=
MIRSAN M?m
MONTAG lo-..
HUNTRANU.Gecm
MUNTIU $??'
MUSAT B0=
MUSETEANU C0
?
MOLDOVANU Go-
NACHTIGAIT. CC=
NASTAO E0=
AUMDc. ,?
NEDELCIU Do-
NEGREANU W,m
NEGREANU Y,?
NERANTIU F.-
NICA Am=
NICOARA I?P
NICOLAESCU 19 NICOLAESC1J _ N o?e
NICOLAU CLom
NICOLAU ST 0cm
2 29 2789 292, 12293489 3719 Me 3,,86,,441
7o6.
3o5, 307
543
6o9
142
412
439.
,591 592
577, 578
N
32 9 339 34?
6o 61 s J. 64+ 6 - 68j ?,2# Toe s
Z2 L6
75% 9-77 ,2. 22 8??q .81, 82., 83
8? :' ; 194, 26o, 266, .272, 403, 4399 ._455.,..
516, 6o9'612* 615, 621, 637, 6390 64o ,698,
4899 495
5459 546
161
585
329
531
372
95, 106
655. . ?
-188.284, 293, 294, 296 .
-Lu 19.2,8, 2.'.
,-.222 .c.?2., 56 v ?2,
-
r
0-
_. -G~ 9 1'2s 10B,
'9...:d.9' Z, #-i u- 102, A
113 114e 124 1 16o# 162e, 22 7
__1749, 175,1 177 a 181 ~182 -_ 19,5~201i L2
23 9 5o 19 ZL2 0 . . ? 1 3 0 Z .56.g 3 9 2 6 1 . t 9 273 284? 2911, 22,9, 9 294) i? 6 a
92, 9 3 .,_. # 30 116.1 44o6. , 2 s 2.. *
.506.L, 2081 1vg 5l ?e. X13-L 514+ 6 _
626,E 63I& 6...322. M" 634 644i 65?.9
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
2!; 66 bog 672-9 673p 674v 6779 A L89 M9
6869 689, ?3o,, 6?21, 6229 694# ,7o8, 71?'
N COLAU So.- 200
NICOLAU V O-.. 594
NICt)1SCU V o- 161
PASCU Lo.=
PALAUE Com 168, 66o
PALADE E o~ 593
PANAITESCU Go@ 178.
PANCU Vo= ,4549 496
PANDELESCU I 594.
0
OANA C 162* 163, 178,399
OLTEANU I, 227.
ONCIOIU PO- 525.9 .526 9 529 .
OFRESCU Eo= 259,98-9_1009 104, .109, 110, 111, 114,__135,
18.3 9 1879 194? 196, 1.979 199, 203, 228 0229 9
2340 259, 315, 3349 337, 403, 455, 605,607,
6o9, 612, 614, 621, 637, 639, 64o, 6619693
OVANESCU AL 132.. 173
P .
PACURAR!, E o= 214.+ 421 * 487, 479
PASCU M o-
PASCU T
PAUL Fod.
PAUN FI,mc
PAULA L0-
PEIULESCU,Y
PESCARU Ao=,.
PETRESCU AL@=
X8505,,5245254 528, 5329 52,555,568,677,
58o,.58:, 585L 86
699
594
499
4,349 453
643
2os2
437
21, . 56,. 292, 348 9,..349,, 350-.9 3519 3539. 356.9..-.
35.89 363, 3759 378, 380, 3819 3820-383.9,.385,,
3869 39o, 396, 397 9 398* 7, 40'6 ,. 411.8412 9
4130 4148 418* 42o.. w p) 4214 4258 426 42794299
43,0, 431, 432, 433, 4400. 441, 44.2, 449,4.53,
454, 46o, 461, 480, 497,_498, 5oo, 543,6839
6959 loo, 711, 712,
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
PETRESCU Ago- 191, 283. 323, 3310 434
PETRESCU P.?== 375.. .
PETRESCU -COMO VG= 434
PETROVICI ..L0= 2o. ?
PETRUSCA J .., 3539 3890 397
PHAM SO1 G o= 471
PICOS Ao=
.385 -
PIRONCOF ALo= 3530
389
PODHORSKI.E 115
POIL? ?M.10= 32
POPA Be-
5489
585
POPA Iod
3979
4110
412.
712.
POPA M0=
1610
1679
18o,
5248
5259
5260
5289
5299 5300
5320
5339
.5440
5689
.5699
.571.9
5766
5779 578,,.
5790
390
4365
4560
472.,
4949
7139
721
POPESCU Attu-
.5190
581,p
583
,
POPESCU GH0=
4o6
POPESCU GDMESCU Go- 1040 1?99 11o9 1110 1160 1359 14oo. 17op
1760 1870 2020 230.0 a 2739 2860 287,
3o2, 3o8, 229 39., 0 9 .1o
POPESCU I o= 217 __
POPESCU PRETA I 168
POPESCU 5810 583
POPPER M0= 161
PORTOCALA R0=.r?x~5~~: Zpo 23b 3e0 9, 41 s
s
4.0.0 51~r r0_, 87n 8805r.1020.101
14
.~1 j ?;,: 224;.s .349
5~2 D 2 2472, 267- 0 279o .2 ~8o 640
~~84 40 46-9 i
444 _q. 446 !tVA 4481 467k -k
oD 122 ojg 6o4s 6o6b' 6444645)
6660 6 219, 6720 6740 6759 6779 6789 679,680,
9 7080. 2 .--,- .... ,
PREDESCU I9=
PRE]DESCU L0=
2000 2o6m 400 .415% 41.60 496.
204 4649 4860 4880 489$ 4900 4,01
PREDOVICIU F0= 167
PRUSKAUER=APOSTOL Be- 435
PUIU Bo= 2519 252
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
+r =
ROMAN Atom 6380 639
RO-AN Poe= 185
ROSCA 1o=' 4.82:
RUSU Fo= 1730 399
RADESCU Re- ,.626, 631, 632
RADULESCU ALo?' 206
RADUL SCU Co- 515
RADULESCU i 5o8 0 5o9, 516, 517
RADULESCU Mo? 374- .
RADULESCTJ. Pb? 2o6. 491. .
RADVAN AID= 13.1, 1430 145
SABARES8 ?Io? -y : , 211 2139 g
43'.50,, 56, 68, 739 129, 208
SAMM 10- 669, 370..0 374o 4-22109 438 i
s327-,3 s
2?2,
NI -2 9
474
448 4.58 0 46 7, 469 474. 5 0
4440 4459 4470 - 4920 6/x60 64 648, 649 ? 703, 7o6, 7169
SANDU Io? 5940 647, 648. . -.
0
SANLULESCU Tom 2o8, 4o2, 404, 437 0 646
194-9 221, 315 * 321 o.? o .
61,-70,769 850 90 ?
SARATEAI~U Do
34.3, 3450 348, 3499 350 s 353 3 35
8 369, 37
36303 fl3.?5366036 _7,369
88' 403 418 4396
,38o, .382 , 383 ? ~ ~-- s
*
4-57o 57.50 588, ? 5940 6oo, 601t.604
456'.+ ? ?
61? Cil 6128613 .614,
?0 60 , 608, 609 0 .a--~- + .---0
621 622; 624; 626, .
,?119,6169,617t
63o a 631, 632, 633; 636t
,o. 629-064o 6410 6420 6641 665 620
637x6380 ~3~0 --- 0
6940 7ao0?70
6810 682@ 683 0 693 8 . +
89 0 3970-.--411 X12. 4.27., 712,
SATDtARI C?? 3530 3
SCHOENFELU S.o? 3o80.613. 642.
CAo~ .95 0 1o6. 182'. 509 0 510# 5110 512 9 513 9
SEPEANU FIRi
5140 650, 652+ 60 SERBANESCU . C 54o-', 5.5? 0 -
SFE'RDIAN 00= 179 04'94 s 721
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 _ _
SIRNU No- -. 521
SOFLETEA So- 331-
SOR0000 Gol 17OD 221, 5720 573s
6280 629o 63o9 6319
5740f7
6329 6330
620
6380
6250
6390
6279
64o0
SORU Eon.
641
910 jl~q 5 667
SPIRU Io= . 122 -.
SPRINCENATU DOOSA E,o=.
663
ST..NOU Aoo
ST.GCA Mod
3530 3890 411
5310 533.. ..
STARK iOT.
2000
4o20
404
STAVRI No?.
27,7
S?.ERESCU Lo=
364
STERE CU.Po-
181.
STIRBU Aoo
27o,
2740
275
STOIAN 10T
2749
STOICAN Vo
527.
STRATI To-
3o8,
613,
642.
S?ROE A0= ,
-.1810
219
691
STOR-P,SCU AoMe- 719
ST?= E? SCU C o? 402
STRCESCU Po-. (see AhanasiuuStroescu
STRULOVICI Yo- ( see Copelovici Yo)
STRULOVIQI D 500. . , .
SUUACI Io.
SURDAN Coo
Po
0' 524 0 0 56 0. 537 0 5.38 m 5399, 0 ... 5.10
5,42 5 6 5~ 5,, 548 0 542# 551, 5530 fl
5566 5576 e 5,59?
582--
?22 0 5ofl 5020 w o 535, 536, .%>8
X00
5390 541,v .5 a 5530 s 561i 562,
,500 o .20o 5?6fl 576 571,0 0 52 ~ q
5750 5770 o X90 5840 62A? 625:8,.62606270
6289.6290 a 631 632 0 633 s. 6 8 0 639 0 64o,
641 D 672
TAGA N0= 2o40 535, 5360 5370 5380 a 54,0 e 5410 5420
551
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
TAINDEL Co- 270, 274, 275, 404
TAINDEL Icc .
TARCHILA D.? $o, ! 1+i 4o.5, 414,
TEODOROVI01 GR.? 265, 277, 364
TIBREA Sea= 5059 532.
TICAN V... , 58o* 581,
TTGAERU N..- 585
TIMERT AAN A. -353.
TIRNOVEAI.Gem 703, 716
TIUCRE.. A.- 64-3.
TOMA A oe ,
TOMESCU V.?
TUDOR I
TUDOR V.~.
TULPAN "'.?
1URCU Io-
UAN TM LI$No?
URSACHE Rom
582
440, 449,
5o3, , 524, 525,
0.69 557, 589, 576, 5789- 582,_582
399
138,.-139, Me 6, 169
98,
95, 106, 185, 188, 189
U
487, 6,13.
548, 5199, 5519 5539
503. 541?, 548, 549, 553, 554, 556 557. 558.
559, 578
V
VACS Le-.. 142,..'
VAINER Ho- 91, 669
VANCOV Za= 161
VASILESCU I om- 6o9... ,
VASILESCU,Pro- 3,W,4, 5,
VASILIU Gm-~ 347:
666, 668
VASILIU N9- 509, 515, 516, 517
VASOIU FL. 288, 3o3.
VATASESCIJ. Ao?' 134, 148, 152, 1669, 686
VELOIU V.= . 634. .
VELICICOV V 235, 242
VILCU?$OJINCA 1.-.453, 498
VIRF L,= 372
VLAD I94 4o5. .
VITA A.o- 59o,'593, 651
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
to 312, 316. '344x 3749 415-9 ?'
' CICULE. SC~" w oa 2 309 a .3 r
41 , 4510 01?+ --+
`VOICULESCU. R.o? 2o
"VO. NESCU I o? 322
'JU1-,jESCU s.-. 323.
VOROSII A oKo= 223
'aCE .R o= 5019 5o2,
567
V EI TRAUB. L 493.
t EC3 + . Rol= 647 0 648
5?os
ZA IR C o-
ZAVATE O o~
W
5o4, 555, 560 , 5619 562, 563 0
52 5??s 532
z
95,.106 .
1.3.2 141, E 158, 162 0
2880 297, 2980 3ok, 305,
?
163, 173, 178
307
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8
 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8
THE WORKS OF THE INSTITUTE
OF JNFRAMIGROBIOLOGY OF THE R.P.R. ACADEMY
(ABSTRACTS) 1949-1961
Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8