(SANITIZED)UNCLASSIFIED RUSSIAN LANGUAGE ABSTRACTS OF PAPERS ON VIRAL DISEASES(SANITIZED)

• Document Type: CREST
• Collection: General CIA Records
• Document Number (FOIA) /ESDN (CREST): CIA-RDP80-00247A004300210001-9
• Release Decision: RIPPUB
• Original Classification: K
• Document Page Count: 143
• Document Creation Date: December 23, 2016
• Document Release Date: February 26, 2014
• Sequence Number: 1
• Publication Date: January 4, 1965
• Content Type: REPORT

Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
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Declassified in Part - Sanitized Copy Approved for Release 2014/02/26
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AKAAEMV15 MEAMU,1HCKWX HAYK
911,CitCPU?14CH1flU.46H,06
AEH VIM-PA./A ?I962
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rCDeclassified in Part - Sanitized Copy Approved for Release 2014/02/26:
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Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
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USSR ACADEMY OF MEDICAL SCIENCES
nstitute
of xperimental
edicine
LENINGRAD. 1962
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AKAAEM1,151 MEAMUMHCHMX HAYli CCCP
HCTI1TyT
Kcnepmmewranbuoii
egisiumHbi
flEHMHrPAA.1962
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OTBeTCTBeHHbIll pe4aKTop
AelICTBIITeTbHbfg ,121eH AMH CCCP,
npogcleccop Z. A. BHPKJKOB
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Heart 17empoeug HABAOB
Ivan Petrovitch PAVLOV
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HCTOPI/11/1 HHCTI/ITYTA
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6
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MECTO BHPYCOB B 1113111304,E
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12
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
IA-RDP80-00247A004300210001-9
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13
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
;IA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
DP80-00247A004300210001-9
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14
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Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
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15
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
',IA-RDP80-00247A004300210001-9
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6eHHOCTb TIHTaHHH HaceKomoro. Penpoayxulln BHpycon B pacreHH,H
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HallaJlbHbIX 3TaTIOB o6pa3onaHnn onyxcoefl.
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16
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Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
HospemeHHo, He .KHHoft npmpo,ae Hilpycos. B3aHmoo6ycaomeHHb1it
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2* 19
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.";1A-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26
IA-RDP80-00247A004300210001-9
HanomHHaloutasi BHpocnopy 6axTepHocPara, Ho c 6coee xpyrawil
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20
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CIA_IR nPRn-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
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pyca. YCT0iII4HBa51 1414pKyTIALIHR HHpyca B npmpoue OCy114eCTBJIHeTCH
caeupoumm o6pa3om: mHoroaeTHHe copHHni (Cirsium arvense L.
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o6pa3om, 6140J10FIIR HiTyea ?Kearryxn 6060H cpawnylecxli He CHH3a-
C HaTIIIHHeM Han 0TCyTCT1314eM xyabTypm 6060H. flpH Hupauw-
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21
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.";1A-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
IA-RDP80-00247A004300210001-9
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?kaHenpo 01101MbIMII ,II?1151 Kaaccuct)HKannil 6bIJ10 BbIBHHyTO BO-
cemb KpItTeplles: 1) mopctionorHH H cnoco6 penpoAyKunH; 2) XIIMH-
mecKtul COCTaB B 4:131H3HqecKHe csocivrBa; 3) HmmyHmormecKHe
CB017ICTBa; 4) myBCTBIATellbHOCTb K (1,1143H1leCKHM 14 XI4M11,4eCKIIM areu-
Tam; 5) ecTecTBeHHHA nyTb nepeJai-m; 6) Tp011143M K X0351HHy,
TKal1H, KneTKe; 7) naTanorHH (BK.moiiag ()opmHpoBaHme BKJ1101-1e-
H4111); 8) CHMTITOMaTOTIOTH51.
flepeqHczeHubie KpwrepHH He HBJ15110TC51 paBHoueHHumli: HapH-
C TaKHMH, KoTopme xapawrepHaytoT OCHOBHbIe 6HOJI0F1P4eCKHe
csoficTBa Blipycos (nn. 1, 2, 4), B 60J1b11111HCTBe 01114 FIBJIHIOTCH
111aTKHMI4 14 HOCHT BpemeHHbal xapaKTep.
4. TjaHHbie 0 mopci)oaornH, xllmmecKom ,cocTaBe 14 pa3mHo)Ke-
111-IH Bupycos xapawrepH3y10T 14X KKK OCO6y10 KaTeroppuo cpeui
,apyrHx npeAcTaBwrerien >KHBon npmpoam.
5. no crpoeHmo BHpycHoil (1HI1 CHMMeTpHH, KO,T11411e-
CTBO Kancomepos, Haffflithe BfleILIHI4X o6onageK) B xHmHilecKomy
cocTaBy (Tun HyKaeHHoBoct KHCJI0TbI, Haamithe 14,114 OTCyTCTBHe
JI1411140B H, OTIlaCTH, cooTHomeHme OCHOBaHHH HyKJIeHHOBbIX KI4C-
.10T) BO3M0)K110 6onee H:111 MeHee onpe,ameHHo BbIAeJIHTb HeCKOJIb-
KO poacTBeHHBix rpynn BHpycoB, npumem neKoropHe 143 HIIX
e,11.14HRIOT BHpycm }KIIBOTHbIX B Bupycm pacTeHHH, BBpyCbI >K11BOT-
HbIX H cParm.
6. floTpe6HocTb pauHoHaabHoci K,T1aCCH1:1314KalIHI4 BHpycos ouly-
maeTcH B npaKTHmecKoil pa6oTe. B CB5I3H C Tem, IITO K0111411eCTBO 113-
BeCTHbIX HayKe BHpycoB Bce Bo3pacTaeT, Heo6xo,L(Hmo pacnonaraTb
23
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
;IA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26
;IA-RDP80-00247A0043002100_0179_
raw onpe?ameaasi KapAaliaabamx
RpH3HaK0B. Taxamll SMARR:ITCH MeTOJ noJamiTeadi penpoAyKumi
Blipycos H m36HpaTenblioe aehcriame HelopTopmx coieci KpaCOK JAR
onpeaeaeum THHa HyKAeHHOBOR KHCAOTM, 1137BCTBRTeAbHGCTb K
4mpy H AeaommoriaTy, Kax cyKleame o HaAHRHH AHHHJOB.
B ycTatioaaelmi 6oaee memarx noJpaa,Jeaeliact HemaaosaKHoe
aaatierme llMT TepM0yCTOR4HBOCTb nim 56?C H CTa6HJIDHOCTID B
30i-ie pH 3,0. Penialompo poab Hrpalur aaTiffeaHme canaa, JAR
BUIRBAeHHR KoTopmx pa3pa6oTanm ycosepareFicTBoBalmbie TOHKHe
MO4I4HKaHHH cepoaormecKax pealima
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26 :
ni A-IR np80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
NI CAH n 1131VI .(11,1414 1314PYCOB
0 MEXAHH3MAX PEI1P0AYKL1,1114 BV1PYCOB )K14BOTHbIX
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MOCKI3a)
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memTaabHme JaHlibie o mexaHm3me penpojyKunn 6aKTepHaabnbix
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npngem o6e HyKfiemHoBbie KbICTIOTbI MOUT 6IDITID KK 0,21110T54)KeBb1-
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1. flpow4KHoBeHme Bmpyca B KJ1eTKy o ,aenpoTern1H3aumH era
IlyKTIeHHOBOt1 KHCTIOTH.
2. FlepecTpoilKa Bmpycom meTa6a3z3ma KjleTKI1 H CHHTe3
111,14)1411eCKHX 3H311MOB, Heo6xo,ammux ,T,AH penpo,ayKumn Bupyca.
3. CHHTe3 HyKJ1eHHOBbIX 6eJIKOBbIX K0MI101IeHT0B Bilpyca.
4. (I)opmmpoBaHHe 3peamx BlIpHOHOB H BbIXO,T, FIX 143 K:IeTKH.
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
.;IA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
JA-RDP80-00247A0043002.100M-,9,a
penpoayx11141A 13Hpyca Ha OT,ThseabHbIe
413a3b1 mo>KeT BbI3BaTb n03pa}KeHnsT, TaK KaK B OJHOCI H T0i4 xe
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Tcmy, 4T0 OHM npoHcxouHT oaHospemeHHo 14 B3aHMOCI3133aHbI me)fc-
co6ofl. 3T14 Ace coo6pa>KeHHH OTH005ITC51 H KU acem .RpyrHm (Pa-
3am, TaK>Ke 110JaHHbIM B yroynHeHHom naaHe.
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ne,uarmo BbI3bIBaJI cnopEA me>1{y CTOpOHHHKaMH r11110Te3 Empo-
nexcHca Ae311HTerpau1u, B HacTonuee spemn 113yHeH ,LIOBOJIbHO
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CTFILILA, KoTopaH npoawmaeTcH II 60J1billecI qaCTIDIO 3aBepmaeTcsi B
HaKywi-H, o6pa3oHamneficH B npouecce ruvonexcHca. Flo-HHAHmo-
My, JIHT1411eCKHe 3H311Mb1 III4T011J1a3MbI, 14 ripe,E[e Hcero KoeTotmoli
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(PeKLIHOIIIIbIX THrp0B, aHTHreHos H
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TIOM KoTopok BbIKJI1011eH HailHOCTb10 HJIH llaCTMIHO. HMeHHO C 3T0-
10 momeHTa JI0 HaHaaa cpopmHpoBaHHH BI4pHOHOB Empyc 51131131eTC51
gyBCTBIlTeJ110HbIM K >KeCTKHM 3J1eKTp0MarHI4THbIM H3JITIeH1451M
(KBaliTaM BbICOKOR 311eprEH).
26
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26 :
ni A-IR np80-00247A004300210001-9
61-000 1-Z00?1700VL17Z00-08dCll-VI',
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Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
IA-RDP80-00247A004300210001-9
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111,11M 113 2200 cy61,e,Eignnu Bgpycgoro 6e.ruca. Mb1 3aKJIK)1111.11H TaK-
Ace, 11TO nopmaabnbie CHCTeMbI C ygacTgem 51,21,ep 11 xaopormacToB
JaruKum cunTe3upoBaTb o6a Tuna LIYKI,b1X 11M 6eJ1KOB Bupycnyto
PHK n 'ITO PHK CMO>KeT aCCOLIPIIIPOBaTb C 6eJIKOBbIMH cy6rbeaugg-
gamg c o6pa3oBangem Bgpycgbix gacTgu, npu yBeT11411e1-11414 npo,ao.ri-
NcliTeJlblIOCTI4 peaKunfi B 6eCKJIeTOLIH0171 cucTeme. 011eBH,1,11b1M CHO-
C0601M npoxiengg CHHTeT114eCKHX peaKugf4 SIBJ151eTCH nenpepbmgoe
Jo6aBJegge HOBbIX ,/,0110JIHHTenblIbIX KOJIHileCTB gopma.ribgbix XJ10-
pormacToB K CHIITe3HPYIOLLI,e171 cgcTeme.
C lle.rlblo nposepKg 3TOI"1 FIIIIOTe3bI 6bIJ111 BbIll0J111e11bI Janbneil-
ume 3KCIICPPIMeIlTb1 H ycTagoBaego, gTo nopmaJmnble XJ10P011.11aCTbI
cpacarm moryT npo,ayugpoBaTb 110BbIe BbICOKOHIICkeKU,H01111bIe Bgpyc-
rime qaCTIILIbI (11aJIOLIKOBH,5,Hble nyx.neonpoTeg,abi) B npncyTcTsug
Bupycnoil PHK.
HOBbIe 110J1110CTb10 megegme gacTggbi Bgpyca 6bmg 110JITIeHb1
npg ,1,06aBTICHHH Pajf,110aKTHBHbIX HYKJ1e0T11,a0B H.T111 aMHHOKIICJIOT.
HO cTeneng BKJ11011eHHH pa,I,H0aKTHBHbIX
MO>K110 3aKJ11011HTb, X.TIOPOI1J1aCTb1 JOCTaB.T15110T 6o.nee, gem 99%,
maTepgaria, ucnonb3yemoro npg cPopmupoBaugg
Bgpyca. Bb1.110 o6nappKego, 4T0 npg ging6augg XJ10p011J1aCTOB
TeMHOTC o6pa3yeTcsi 3HaLIIITeJlbH0 megbuiee KaT1141-1eCTBO HOBOTO BH-
pyca, gem npg unxy6aung Ha CBeTY. DTO CBH,aeTeJ1bCTBYeT 0 TOM,
clgrrocHnTeTggecicue npoueccm moryT JOCTa13.1151Tb aMHHOKHC.110-
Tb1 11 gymeoTg:tbi 6onee megee nenocpeAcTBengo JAB peaKugil,
CBH3aIIHbIX C CHIITe3OM Bgpyca.
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.106aBfierigg IleHHH,HJIJ1HHa, cTperrromgugga H MHKOCTaTHHa. B ga-
cTogntee Bpemg negcgo, B KaK0171 cope gaxo,amTcp aKTuBna5-1 B14-
pycHasi PHK-maTputa, HO MO>KHO 11Pei1,110.10>K1ITb, TITO 310 noBao,
29
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
.',IA-RDP80-00247A004300210001-9
CHX Hop HeH3BecTHaH cpopma, xoTopaH ycTofiluma K pH6oHyx,rie-
a3e. Heo6bigailHo 11,110THbIe, posHme HaJIOTIKOB14,11,HbIe cpopmbi yilaBa-
.710Cb Ha6moilaTh HpH H3ytieHHH CIIHTe314pylOWHXCH npenapaToB B
3,11eKTp0HHOM MuKpocKone, I1p1411eM HHIPeKIIHOHHOCTI3 BHpyca oxa-
3bH3aeTcH CBH3aHHOfi C 3111MI1 cPopmamH.
Pe3y1IbTaTb1 naHnix 3xcnepliMeHTOB CBH,leTeJIbCTByfOT 0 B03-
M0NKHOCTH cHHTe3a BHpycHux B 6eCKJIeTOTIHOH cHcTeme.
3THMH HCCJIe,a0BaHHHMH BriepBbie HpoTkemoHcTpHpoBaHa B03MOAC-
HOCTb noayqeHHH 1eJIbIx BHpycHux TlaCTI1 BHe )1{14B0c1 KJIeTKH 14
noxa3aHo, TaKHM 06Pa30M, TITO npottecc cpopmupoBaHHH BHpyca He
51BJ151eTC1I BHTaJIHCTIlileCKOCI Tarma, a mo)KeT 6bITb onHcaH 6110-
XFIM1114eCKH Ha OCHOBe H3BeCTHbIX upeAcTameHHA 0 6H0CHHTe3e
6e,nma H HYKJ1eHH0BbIX KHCJIOT.
HEKOTOPME 3AKOHOMEPHOCTI4 PEIIPOAYK11,1114
PHK-M111(COBIWYCOB
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MocxBa)
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CTB1414 aKTHHOMFILOMa,1i,Bcrynarowero B cTa6HabHpo CBH3b C ,L[HK
H no,rtaHamoutero CHHTe3 PHK-HOJIHMepa3bI.
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mHoxcaacH B KJIeTKaX xyabTypm TKaHH B npHcyTcTBHH -rex KOH-
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2 qaca. MeTo,Lkom aBTopa,z(HorpaPHH C HCII0J1b30BaHHeM H3-ypHAHHa
30
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
6bmo yeTanormeHo, anpyc Bb13binaeT n0a13.newie cHHTe3a K.ne-
Toithon PHK tiepe3 2 qaca Houle 3apa>KeHHH, a qepe3 3 qaca HaqH-
HaeTcH HHTeHCHBHbIfl CHHTe3 supycHon PHK B 51,apbm1xax, ripeKpa-
matoutHficH K 5 qacam. B Kyawrypax, o6pa6oTaHHEilx aKTHHOMHU,H-
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1 '-lac Home 3apa>KeHHH.
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ne-
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3yAbTaTe mem pH6ocombi paHmue HaIIHHalOT 4PyHKII,HOHHp0BaTb B
COOTBeTCTBHH C reHeTwiecKon Hporpammon Hllpyca.
B OTTIHTIlle OT 13Hpyc0n CeHaaci H Nov, aKTIIHOMHUHII OKa3bIBaJI
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HOMIIIIHHa He cpopmuponaancb remarrmoTHHHHE4 BHpyca rpunna,
Ha OCHOBaHHH tiero Bappn i Apyrne ripnwall K 3aKJH011eHI110
o6 yqacTHH B penpoayKunll Bnpyca rpunna K.TieTotmon ,LIHK H,
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HO CpaBHeHHIO C HellyBCTBHTeJlbHbIMH K aKTHHOMMIHIly BH-
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pycy rpHrina Tuna A PR8 B KJIeTKaX Ky.ribTypEd TKaHH, o6pa6oTaH-
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jaAH C JaHHbIMII BHpyco.normecKoro THTponaHHH. 0,a,HaK0 HH4peK-
ilHOI1Hble THTpbI BHpyca npu 3TOM 6bIJIH CHH}KeHbI He3Ha4HTeJlbHO.
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tieHHH H3-ypHAHHa B H,apo H 0co6eHHo 3Hal1HTeJlbH0?B HApbauKH;
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npe,g,e.rieHHe B HitepHmx cTpyKTypax.
He 6buio BUSIBJleHO IleTKOe yneameHne pa,alloaKTHHHocTH B ne-
pHoA cHHTe3a PHK Bnpyca PR8 H B KucnoToHepacTsopumon 4>paK-
11,1114 nepe>KHnatoutHx X0pH0aJIJIaHTOHCHbIX o6o.notley Kypimbix 3m6-
p1101-lon npu HCI1011b30BaHHH C14-ypwinia. 0,1,HaK0 B TOM Cay'-me,
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ule 3apa>KeHHH, B TO BpeMi KaK H3meHeHHH pa,II,H0aKTHBHOCTH B
ne o6pa6oTammix aKTHHOM141114110M mem6paHax Ha6.rnozta.nHcb JIIIUIb
qepe3 21/2 tiaca nocae 3apa>KeHHH H no3>Ke.
34
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
DIA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
IA-RDP80-00247A004300210001-9
TaKHM o6pa3om, cHHTe3 PHK Bnpyca PR8 He TOJIbK0 He 6bm
HoJaHneH aKTHHOMFILIHHOM, HO, KaK H y BHpyea Ceujai, HacTynaa
B 6o,Ree paHHHe epoKH. AHa.norHqHme pe3yabTaTBI 6bIJIH limy-fern:A
lia MO,ReJIH BHpyea KoaccHgeeKoh tiymbi nTHH.
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permuKa3of4), 4TO H y BHpycoB, yeT0114HBbIX K aefICTBHIO aKTHHO-
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npn Ao6aH,r1eHHH aKTHHOMHIMHa He no3>Ke gem Liepe3 1 tin Home
3apa>1ceHHn; 3TO jaeT oeHoBaHne npeano.naraTb, 4TO Hapymeaaa
cHHTe3a BHpycHoro 6e,TiKa 06yCJIOBJIeHbI HapymeHummH BO BHOBb
cHHTe3HpoBanlloti moeKyoe BHpyeriofi PHK. 110-BHJHMOMy, aKTH-
HOMHHHII Bb13bIBaeT KaKHe-AH6o H3meHerai5I B uneTpone PHK, onpe-
,aemnoLuem erHITe3 BHpycHboc aHTHreHoB.
06 OAHOM THIIE B3AHMO,ITERCTB1451 BHPYCOB
B KJIETKE
B. If. A2o.4, I'. A. 111 11 pmau
(FIHCTIITyr 110,711i0MHOOTITa H mipycirux afiqeepaJnIToB AMH CCCP)
Kacpe,pa Bilpycozormi mry, Mocium)
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PHK 0,g,H0F0 Bpip yea 3a etieT PHK-nwiHmepa3m, HH)1,yunpoBaHHori
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oTspaluaeT o6pa3oBaHHe PHK-no.immepa3m y ryaHH,Euin-liyBeTBH-
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)114M LIAR o6pa3oBaHH5i 3Toro cpepmenTa y ryaHH,IIIH-3aBHCIIMbIX Ba-
pHanToB, 1-103TOMy eemi KJIeTKy 3apa3HTb o,aHoBpemeHHo 0601-ImH
BapHaHTamH B npHeyTeTBHH ryaHH,EiHna, TO HHayunpoBarb 06pa30-
BaHme PHK-no.rinmepa3m emo>KeT TOJIbK0 3aBHCHMIDIr1 BapHaHT. 06-
pa3oBaHHe B 3TOM e,nytiae BnpyeHmx LiaeTHH, OTHOCHIIIHXCS-I K tlyBCT-
BHTeabHOMy Tnny, mo>KeT roBopHTB 0 TOM, 4T0 PHK-HOJIHMepa3a,
HH,rtyuHpoBaHHan 3aBHCHMbIM BapHaHTom, mo>KeT HCHOJIb30BaTb B
KageeTBe maTpHum PHK gyBeTHHTeabHoro BHpyea. C ,apyrofi CTO-
poHm, ecin emewaHHaR HHcpeKuHn 11p0HCX0,1HT B oTeyTeTBHe ryarnt-
aHHa, TO peripo,ityKuusi 3aBlICHM0F0 HapHaHTa eBurkeTe,mbeTByeT o
TOM, 4T0 OH o6pa30Ba.rien Hp!'! yqacTHH PHK-noaHmepa3m, HI-Laylill-
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wHmH K nepHomy HmmyHariorwieeKomy THny BHpyea nanHommermTa.
32
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
nIA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
06pa3oBaHHe OJIHWeK 0,1,1111M BapHallIOM (Fya1ii4,11,HH-IlyBCTBHTeJib-
IIHM) pe3KO TOpM03H1I0Cb B IlpHeyTCTBHH ryaitu,KuHa, Tora KaK
peripoAyKunK BT0p0F0 napllawra (ryatm,anit-3askic1'1moro) npoucxo-
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BH11 ryaiin,amia Tip014CX0)1,14T oopa3onaHme 3aMeTHIDIx KOJ1HtieCTB
ryaintanH-liyHeTnnTeabitoro sitpyca, XOTSI ripu HJ1OFHHbIX ycao-
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libici sapHawr ripaKTpitiecKH He 06pa3yeTc5l. B OribiTax, FIOCTaBTIOH-
Hbix B OTCyTcTBTle ryaHH4Hila, 6bIJIO OTMelleHO elite 6o.ribiliee uritmy-
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3anucHmoro 3Hpyca.
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cyTcTBHe a6comoTtioft crieuHciminiocm HH,IyumponaiiHoft BHpycom
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no ripe13apHTeabHb1m JaHHUM, B orrcyTurime ryaHHanHa
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0,1,Hospemeitnon Hitddemuni ryailizi,111H-LiyBCTBHTeablibim BvipyCOM II
THlia. Flp0B0,1HTC5I HMMyHOJI0rHmecK0e HarieHHe BHpycHbix macTHH,
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CHHTE3 PHK BHPYCA
TAB/HW' MO3AHKH IN VITRO
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i13rietini4 CIIHTe3a BupycHoci HHCPeKHHOHHOA PHK B 6ecKlieT01-111bIX
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? npouecce penpo,LiyKuitH in vitro capKin supycHasi PHK H 3TOT
CHHTe3, He3asticHmmil OT JHK KJIeTKH X035:11,1HB, oeymecTimisieTcK
3 Te311Cbl AOKIla0B
33
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
IA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
'.,IA-RDP80-00247A004300210001-9
npn nomouth r0TJ11411110r0 OT PHK-110.1111Mepa3b1, 14)epmenTa, a Hmeu-
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onpeae.nRembal cnexTpocpoTomeTpmecith, HacTyna.ri B npefle.riax 40-
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KJIETOK, HHKYBHPYEMbIX C HYKJIEHHOBbIMH KOMEIOHEHTAMH
BHPYCOB 110JIHOMHEJIHTA H AolEHOBHPYCA
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(Alocxoscitnn BayBno-BccnenoBaTenbcfmn HHCTUTyT
Bmpyclimx npenapaToB)
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Te3a 13Hpycon B meme Tpe6yeT pa3pa6oTKH 6oaee npocTux moae-
aen, pa3peniatomax H3ygaTh oTaeabHme CTaAHH 3T0F0 nponecca
OTKpITUX cHcTemax.
flonuTKH H3ygeHHB nyTeft 6HOCHHTe3a KOMHOHeHTOB 13Hpycon
6eCK.TheT041-1131X cHcTemaX BcTpegamT 3Ha4HTeobHme TpyaHocTH
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B nponeaeHHmx HCCAeJ10BaHHBX pa3pa6oTaH meTozi, 1-103BOJIHIO-
EHHII 143TlaTb nponeccm HHAyinvyemoro impycHoft PHK CHHTe3a
34
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA- RDP80-00247A004300210001-9
6eJIKa 14 IlyKaeHHOBbIX KHCJIOT B paipyilieHIlbIX KJ1eTKaX B TelieHHe
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cTumpthpyeT CHHTe3 6eJIKa H HyKJIeHHOBbIX KIICJIOT B panymeHumx
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pe TKaHH (Py61111 Ii coTpyaHHKH), a TaK>Ke o6 HirrepctlepeHumu.
3*
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
IA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
',IA-RDP80-00247A004300210001-9
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(0Kep-Bpom H coTpy,aHm(H).
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104 0a50 310 ,u,ef1cri3He npaKTHilecKH He npommffeTcH; ripH MeHbLTIFIX
36
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Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
Ao3ax Ha6,niouaercH ovieunisoe Topmom.(eHme (PopmnpoBaHHH ony-
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BlieJemnbai 3a 10 "weft Jo 3apamemuf BCP, He nposlimsla 141-1F14611-
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aKTHBHOCTb BbI41B14,11HCb, npuilem maKcHmym ee na6moJa,mc51 npH
HseJeHHH Hnpyca rpruma 3a 0,4,14H ,11,eHb Jo BCP, op,Hospemenuo C
BCP, a TaKmke gepe3 0,E114H-JBH AH5I nocne BCP. OTHeTJIHBOB Top-
moNceHne o6pa3oHamT5T onyxo.geti oTme,TeHo npn BBB,14e111414 BHpyca
rpHnna uo 6 Judi noc.rie BCP.
B TO ?Ke BpeNIA nne,leHHe BHpyca rpunna noefie nepHbix maxpo-
CKOHIALleCKHX npu3naKos HOHBABHHH onyxoan He npHHojHao K 3a-
me,I.nemno ee pocTa.
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panumx sTanax pa3HHTHH nporpecellsi capicomm Payea npoHcxouHT
3a .cileT 113mHco1{enHn Bapyca (aToT npouecc 6.rioxlipyeTen Hllpy-
Com rpHnna); Ha 6o.nee I103,11,14HX 3Tanax ynefunieHne onyxo,To4 npo-
HCX0JHT npeumyweeTHeHllo 3a eqe-r pa31\4110>KeHHH K.TleTOK (Ha 31'01
npoueee Hupye rpHnna He Bongo).
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
JA-RDP80-00247A004300210001-9
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Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
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41
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Declassified in Part - Sanitized Copy Approved for Release 2014/02/26
JA-RDP80-00247A004300210001-9
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-42
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rIA-R nP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
a.T151 BiTyca no3eaeiteHus1 L1BeTK0B iutesepa noHa3aTb TpaHco-
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K. Cnuc
(HHCTIITyT impycoaormi, Beimmti)
HOBb111 po,3, Hupycos H3OJIHp0BaJIC51 qacTo 143 Hpoi3H genose-
ha B CJIrla/IX 3apa)KeHHH renaTHTom. B TxaHeHoil xyabType OH11
11p0HBJ15110T 3ameTHy10 ariToHHTepcpepeHutuo. Blieunial BHA 3114X
HHpycos H3yliaeTcH in vitro c Heabio BblACHeHHH mexam43ma ca-
m0itHrH6np0saHHH VI BbIHBJ1eHHH cliawropos, Bb13bIBa1011114X 6J10KH-
posativie Hilpycori, a TaHx(e 110,II,X0,RH11114X cpertcTH ,T4,1111 npeol&oJie-
Hilli ByrteT o6cy)KrteHa BO3MONmaHHe PHK 3HaLIFITeJ1bH0
mo,Te6.1eTcq B aapa.?meHHog TKaill4 HO CpaBHeHHIO C moHTpoaem.
Tax, qepe3 4 qaca nocae aapa)meHHH HmeeT mecTo cyngecTseHHoe H
ilocToBep Ho e cHmmemge, a qepe3 6 qac.-3/HemmeHme coaepicaHHH
PHK B Lorrormaame maeTom 3apa2fceHHofi my..THDTypm. K 10 qac.
coAep>maHHe PHK B HHToma3me H4Humposagnibix KJICTOK CHOBa
na,aaer. B HApbaumax K.leTOK 3apaxwfaioil xyabTypm KO,T11111eCTBO
PHK CTaTHCTIILleCKII ,I,OCTOBepHO cHkumaercH qepe3 6 qac. Houle
3apaxceHHH.
B ,apyrofi cepHH OflbITOB 6bum ycTaHoH.11eHo, 11TO II0Ka3aTeaki
co,Repimagnin PHK B uguronaanie cmopmeHHbix JereHepHpyrommx
maeTom tiepe3 24 qaca nocie aapa)memgq He npesbnuagoT TaKOBbIX
K.leTOK KOHTp0,TIFI.
Koae6aHmg coaepmaHHH PHK H HuTormaame H R,apbunmax
K.leTOK B nepHble CyTKH Houle aapa)meHHH HaX0/IHT cHoe o6.bsic-
Hemge B cHeTe coHpemeHHbix npeacTameHml (B. M. )KaaHoB,
1963) o CII0C06HOC111 PHK-coAepicaumx HHpycos BbI3b1HaTb ocTa-
HOBKy cHwreaa migeTotmoil H4opmaggHoHH0il PHK B 111(IJ111111p0-
BaHHbIX maencax B cambie pane Cp0K11 Houle aapa)meHHH. Mom-
HO npeArganaraTb, qT0 BTO 06CTO5TTeabCTBO 06"b5-ICH5TeT CH14)KeHHe
coilepmaHHH PHK B Hifromaame m.neTox qepea 2-4 qaca HOC.111"
3apa)megn4q. flosbuneHme cozgep)KaHHH PHK B uHTormaame maerox
qepea 6 mac. Houle aapa)meHHH 3aBHCHT, OTIeB11,11H0, OT Hommel-my!
K 3T0My epoxy B uHTonaaame KJ1eT0K BHOBb upopmfgpoBaHHoro
HHpyca, a Taioice BbIXO,TIOM PHK H3 slitep KjIeTOK B 1114T011J1a3My.
nOC.T1eAHHM mo)meT 6bITb 061.51CHCHO CH1NKeHHe coAep)maHHH PHK
B H,apbnumax. C1111)KeHHe KOJIHtleCTBa PHK B TgHTon.naame KJIeTOK
qepe3 10 qac. Home aapaxceffign, BO3M0)KHO, C135-13aHO C HallaJlOM
BbIXO,aa BHOBb c(popmHpoHaHwerocH Hllpyca 113 KJICTKII,
44
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
nIA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
HpezieTaBAHeT HHTepec HccaeiloaaTb npu nomou_tH meTo,aa UM-
TocpoTomeTpaa K0ae6aHHH coziepicaHHH PHK B meTicax ripn aa-
pa?Kenin kix PHK- 14 JIHK-coziep?Kanikimm Hupyeamii.
HEKOTOPblE 43EPMEHTATHIMblE MEXAH143Mbl
BHYTNIK,TIET04H0F0 BHOCMHTE3A 1314PYCOB
X B. I oity6e6
( 1'; ik p co,lorimecKasi aa6opaTopHs ,qemnirp.a,acKoro Bayytio-liccae,loBaTe.mcKoro IIHCTIATI\ Ta
a KIIIIH 1 Ch130pOTOK)
01-IHM 143 aaAcHefiunix HaripaaaeHHH B 1-t3y4eHHH ripmpoziai BM-
pycoa 1iB,I151eTC5I Hecaeziosaime cpepmewraTHaHmx mexatill3mos FIX
anyTpllmeToimoro 6HOCHHTe3a. cospemeHHaim ripeacTamenii-
AM, anpycHaH tiacTilua He pacno,naraeT CBOHM Haoopom 43epmeH-
TUB, CIL00061113IM OCyLU,eCTBHTb CHHTe3 HyKaeHHOBIDIX KHC,TIOT, 6ea-
KOB H zipyrilx cTpyxTypHaix KOMTIOHeHTOB Bapyca, a TaK)Ke 3Hep-
reTH4ecKH o6ecne4Mvra 3TOT CHHTe3. B CBH3H C 3THIVI ace yKa3aH-
Hble (PyHKILHH Bb1110,IIHHIOT ipepmeriTbi KJIeTKH-X03HHIla, a atipycHaa
Hyx.meilHoaail mucooTa axTuakipyeT CHHTe3 KJIeTOLIHbIX cpepmeHTos
HRH ziax{e BbI3bIBaeT CHHTe3 HOBbIX, He ripncylmix ziaHHoci KlIeT-
Ke 3H3HMHbIX cucTem, TIOCKO,TIbKy 3T0 Heo6xoziHmo AAR ampycHoil
penpoziymiHn ()Kzianos, 1963, 1964). OzinaKo KoHxpeTHaie (Pep-
meHTaTriaHme mexaHHambi 6HOCHHTe3a aupycoa HarieHai HeziocTa-
Totnio. B IiaCTHOCTIL, cosepuleHHo He yilliTbisalorcH cIJePMeHTaTHB-
HbI?H OCO6eHHOCTH o6meHa HeuiecTs TKaHil, B xoTopoil
npoHcxo,RHT anpycHaH penpo,ayxumi
B pa6oTax CmHca H KyHa (1950-1954), MaTueaazia H
(1958), AHamixsnia H BapoHHa (1959), KyHa PI zip. (1960), Keji-
1H 1-1 (1961), resayziaila (1960, 1961), ,171),KH..3b6epTa 14 up.
(1961-63 rr.), Foay6eaa (1963), roay6esa H zip. (1964) epepmeii-
1'a-rumble e,Eimirm npH BHpycnoti HHCPeKILIIH H3yila.rim nyTem He-
nocpeAcTseimoro onpezi,eoeHHH (1)epmeHTaTHBaori (aabzialia3Hoft,
4lociporeKcoH3omepa3Hoil, naKTHKoziernziporeHa3Hofi, TpaHcamu-
lia3HOil 14 T. ,IL.) aKTHBHOCTeil B xypimaix 3m6pHoHax H TKaileBbIX
KyobTypax, H41411,Hp0BaHHbIX pa3,TIFItifibIMI4 aupycamH. Hamu ye-
TaHomeHo, 11TO UH penpoziymumi PHK-coAepKani,Hx BHpycoa na-
poTHTa H noanommemiTa B KyJiaType (1)H6p36JiacT0s xypnHoro
LIeJLOBelleCK0r0 3M6pHOHOB COOTBeTCTBeHHO aKTHBHOCTb a5b,E1,0J1a3b1
B xyabTypaTIbH0fi }KH,LLKOCTH He IlOBbIlllaJIacb, XOTH 06a impyca
Bb13bIBaJIH 11,HT011aToremible H3MeHeHHH H aKTHBHOCTb itioccPoreic-
cou3omepa3bi B 060HX CJIyilaHX 6ahria nosanueHHoti. C zipyrok
CT0p0Hb1, npu peripoziyxmai yica3amibix Bapycoa Ha 311HTe.TIHaJIblIbIX
45
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
3IA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
DIA-RDP80-00247A004300210001-9
KaeiKax 411014KH mopcKoH 0314HK14 FIJIH geaosellecsoro 3m6pHoHa)
B syabTypaobHoh )4(14TIKOCTI4 110BbILLIaJlaCb aKTHBHOCTb 060HX cl)ep-
MeHTOB, TK Ace KK 14 HH penpo,Etysum4 ,UHK-co,rtepmcauthx aae-
HosHpycos 3-, 4- 14 7-ro THHOB B TKaHFI 4PH6p06nacTos KypHHoro
3m6pHoHa (IIOCJIe,T1H14i4 Cf)3KT BbIHCHeH B COBMeCTHOri pa6oTe
A. A. CeaHsaHosum. PlaM AMH CCCP).
IlpH4HHb1 pa3JIHITHR aKTFIBHOCTH OTAeabHbIX (1)epmenTos npu
penpo,ityKuum sHpycos B KOHKpeTHbIX 3scrieppimeHraabHux ycao-
BiffiX no,vieH(aT cneuHaasHomy H3yHeHmo, HO HeCOMHeHHO, HT? OHII
o6ycaosaeHbi OCO6eHHOCT511v114 s3aHMOOTHOIllefilik supycos H KJIeTOK
B npouecce pa3BETHH supycHoil HHCPeKI4H14, Hp14B04I54444efi K CHHTe-
3y ,laHHOFI KJIeTKOPI sHpycHbix tiaCTI414 B COOTBeTCTBH14 C 3aAaHHoil
reHeTwiecKoh HH(popmauHeR. flocae,i1Hee Ho.losceHme 6bmo npo-
HamocTpuposaHo JaHHEAMH crieuHaabHoil pa6oTbi (rally6es, 110-
,154K, )KF1,110Ba, 1963), B KOTOpOn o6Hapys(eHo, Lrro (PepmeHTanis-
Hbie CABHI14 HmeloT mecTo B He4yBCTBHTe.11341b1X K supycy TKaHe-
BbIX syAbTypax, HHOKyJII4p0BaHHbIX sHpycHbimu PHK.
DTH C,T4BHril Hp1414141-1H0 CB513aHbi C CHHTe3OM sHpycHbix Hacniu
H COOTBeTCTBylOT cpepmeHTaTHsHomy riporlmrno aaHHofi TsaHH.
Pe3y.nbTaTbi H3y4eHHH cpepmeHTaTVIBHbIX C,LI,13141'0B Hp11 supyc-
Hai penpoaysHHH pacKpusalOT OCO6eHHOCTH aHmexTHIlecKoro
s3aHmo1lefIcTsHH KOMII0HeHTOB CFICTeMbi BIpyc-saeTsa HH supyc-
Hon 114eKill4H H npoTHsopetiaT MHeHHAM 0 Ha,1141114H y BHpycos
caMOCTOHTeAbH0r0 oomeHa BentecTs.
143PIEHHE MHOTP011HOCTI4 BUPYCOB KOKCAKH
METOAOM MOMPIHECIAIWY1011114X AHTHTEJI
10. A. Eapuimetitt
(KHeBclinfl Harnio-mccaeAotiaTenbmiti IfliCTIITyT 9nHAemuo1ormi
Nampo6Honortm)
OJAHOFI 143 6H0J101-1411eCK14X oco6eHHocTell Bilpyca SIBJIHeTCH era
C110C06HOCTE, HospesukaTh Te HJIH Hume TKaliH, HTO BX0,24HT B 110-
H5ITHe TKaHesoro Tporm3ma. MopcpariormecKHR aHaoH3 TaKHX
HopasceHHA ripe,acTaBAHeT Hwrepec He TOJIbK0 C TOIIKH 3peHHH llx
oco6eHHocTeu H OTBeTHOci peasHHH TKaHH, opralla, opraHH3ma Ha
nospesuteHHe, BbI3BaHHOe sHpycom, HO H C TOLIK14 3peHHH 6Honorm-
Hecxxx oco6eHHocTeil camoro sHpyca.
HecmoTpH Ha TO, trro 3a nome,sHee apemH B Hamen H 3apy-
6escHon AHTepaType HoHsJimoTcH coo6uleHHH o nopa)KeHHHx sce
6onsulero tHicJIa opraHos H Txa B KOTOpbIX o6HapystHsalurca
mopcpanorwiecsHe H3melleHHH, Bb13BaHHbIe sHpycamH KOKCaKH FI
46
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Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
cnexTp Tp0IIHOCT11 3T1'IX Entpycos Bee Ocoee pacwHpReTcH, nopa-
>KeHHH CKeJ1eTHbIX MbIIIIL4 Hee ?Ke ocTatoTcH Beaytunm 14 HaH6onee
THI1 141-1HbIM 11p143HaK0M ,11,aHHOIA 14 1-14leK11,14H.
061DeM Call (14y3HOCTb Halt ogarosocTb) H TonorpagmH nopa-
Ketntil, a He xapairrep H cTeneHb allurpoct)tntecKtix H3meHeHtal B
mumetntom scooKHe c.IpKaT Kpwrepnem /4,151 onpe,LteaeHHH rpyn-
noBai HpInta,itae)KHocTH BHpyca KOKCaKH, Bb13BBIflF0 Te HJI
HHbIe nopa?KeHHH.
MHOTp011HOCTb Hupycos KOKCaK14 J1e}KPIT B ocHose Komnaexca
KJIIIH14,11eCKHX nposmitemici, oco6eHnocTeil naToreHem H mop:1)0-
aormecKoro cy6cTpaTa HHcPeKtutH. Hanpumep, B OCHOBe ATICIIHO3
H paHo pa3ButBatouterocs1 unaH03a KON{HbIX 110Kp0B0B y COCyHKOB,
KK Ham Ka}KeTcH, ae?KaT uncTpocimtgecxue H3meHeHnz H ru6e.TH.,
Mb1111e4HbIX B0110KOH Jutacimarmbi H mexcpe6epHbix MbIllIll, a TaK-
)Ke Hepene nopaxceHHH mnoKapaa.
MHorointcaeHHumn HCCJIeJ40BaHHHM14 6b1.110 noma3aHo, qTO Hu-
pyc KOKCaKH A, nortaaaH B opraHH3m aKcnepnmeHTaabHoro }KI4-
BOTHOTO, BbI3bIBaeT noHpokitemie 14 rn6e.rn) mbnuenHoro BO-
aomia C nocJieupow,Hm peaKTHBlimm BocnaneHnem CO CT0p0Hb1
14HTepeTHHH1T.
Mopcko.riormeckne 0006eHH0CTH noripeKueHtici mbuuetmoro BO-
J1OKHa, H3yitasumecH HamH rucToaorHmeckH, THCTOXHM1111eCKH, 6no-
xmmli4ecKH (co,Lteptcamte M11O3HHa A n B) 14 ummyHanorngecKH
npH 110M0114H mommHectutpytountx aHTHTea moryT C110C06CTBOBaTb.
110H14MaHHIO HeKOTOpbIX CT0p0H 614o.nornitecKHx oco6eHHocTeii BM-
pycoB KOKCaKH.
B npouecce nepepoKEkeHHH MMILIegHOTO HarloKHa H Hem npo
IICXOJIHT H3MeHeHH54 6140XHM144eCKOTO nopHitKa Hoothue H3meHe-
Hne OCHOBHbIX crpyKTypHbix 6eax0B mmuteintoti TKaHH (MH03 14H A
H B), B itaCTHOCTH.
06Hapym.ceHne aHTHreHa npH 110MOIB,F1 aftrilTeaa, K xoTopomy
npitcoe,EiHneH tihriyopecunpytouutil Kpactrre.rib KyHca (Coons A. H.,
1955) 6bia BriepBble npumeHeH B 1956 roity (13.11HKom, Xo.abuepom
H Maptuaa.nom (Finck H., Holtzer H., Marshall J., 1956) ?lag.
143yLleHHH 60KpaTHTeJ1bHbIX 6eJIKOB B 1130J1Hp0BaHHbIX mllocl)H6pHa-
.Tiax. Bbipa6oTaHHbie aHTtrreaa Ha Mbimeinibie 6e,,TKH, ncno.m3ye-
mme B KagecTBe aHTHreHos nocae o6pa6oTHn HMH mbnueimux 4)146-
pHa.n, o6Hapy>tutHatoTcH no cpnyopecueHunn.
143 CKeZeTHbIX Mb111111, 6eJIbIX mbnueg Hamm 6bIJ1H BbI,E1eJ1eHbf
MHO3HH A H M1403 I4H B no HeCKOJIbK0 113meHeitHoil meTo,akuce CLIRHT-
T1140p,abH (Szent-Jyorgui). AHTHreHamH, 11p14FOTOBJ1eHHb1MH 143
cKeJteTHoci mbnuum, mHo3HHa A H MHO3HHa B, ummyHH3Hp0HaJ1H
KpOJIHKOB 110 cxeme, npeitaoNceHHoil B. H. Hocbcpe. floayiteHHHe
affrnmbanetiHme H aHT11M11O3HHOBbIe CbIBOpOTKI4 143yitaaticb cepo-
aorHitecKH KK C TOMOTIOr144Hb1M, TK 14 C reTeponortomum aHTHre-
HamH (neiteHb, nomKa, ce.ne3eHKa) B peaKUHH CBH3b1BaHHH KOM-
noemeHTa.
47
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:DIA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
nuaygetnibix rao6yaHHoBbix cbpaKHHA CbIB0pOTOK C
1 130TH01414aHaTOM CPayopecuelia 11p0H3B0,114J10Cb H. H. M3HbK0 HO
BH,RoH3meneHHok' meTo,IHKe Mapinan,qa (Marchall J. H.).
3KCIIepHMeHTaJIbHa51 KOKCaKI4 14H(PeKIA1451 BOCTIp0H3BOaJ1Aach
Ha cocyHmax 6eamx mbinieh B Ho3pacTe ,ao 24 qac., 110,11KWKHLIM
3apa>KeHHem FIX B acme 0,03 MA 11pOTOTHIthb1M LuTammom BHpyca
KOKCaKH A8, KOTOpIDIfi B pa3He,aeHHH 10-7 BbI3b1BaJI THITHITHbIe
y COCyHKOB Ha 3-11 AeHb Houle 3apa>KeHm5I c aera.TH,Hum
Hcxo,lom. B onbrrax HC110.11b30BaHO 200 COCyHKOB, 110J10B14Ha 143
KOTOpbIX 6bIJIa 3apaKeFia Bnpycom, a 100 ,apyrux cAy>KHAH aKa-
tiecTHe KOHTp0J151.
,JJ1 A rHeT000rmecKoro HccaeAoHaHHH B AHHamHKe COCyHKI4 3a-
61113a.TIHCb Ha 1-e, 2-e H 3-14 CyTKH Hocae 3apa>KeHHH, TyLuKH 14e-
jIHKOM CP14KCHp0BaJ1 14Cb B 10% pacTsope HefiTpaabHoro cpopma,1H-
Ha Ha xmo,uy. Houle HapaitHHormii 11p0BUKH Hape3aall cpe3bi
T0.7111THH01 B 5-6 At/C. Cpe3hi ,rtenapacimHHpoHami, o6pa6aTbmax4
COOTBeTCTBylOLIIHMH CP:IyopecuHppolullmn chmopoTKamH H npo-
cmaTpuHaall B muKpocKone Mil-1 CCOOTBeTCTBy1011414MH CBeTOCPIIJIbT-
pawl.
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ii HeoaHopoaHo. HpH coxpaHeHHH nonepetmoil HcqeptleHHocru cHe-
qeHHe o6Hapy>KHHaeTcH H HH,qe cHerquwxcq no,noc, qepeAytollulx-
CH C TeMHbIMH noaocamH, 0,aHaK0 Ha Hcem 11p0T51>KeHHH BoaoKua
pasHomepHoe cHeqeHHe.
MbnueqHme B0J10KHa 3apa>KeHHbix COCyHKOB CBeTATC51 6o.lee
IIHTeHCHBHO, HO HepaHHomepHo. 4epe3 CyTKH Houe 3apa>KeHH3,
C HoTepe1 HonepenHoil HcqepqeHHocni, LITO xopoluo BITLIHO Ha
ripenapaTax, oKpameHHblx reMaT0KCI4J11411-30314H0M, TepHeTcq OHH-
camiasi y He3apa>KeHHux }KHBOTHbIX ceTqaTocTb c qepe,aoHaimem
CBeTAILIHXC51 14 HeCBeT5IIII14XC5I noaoc.
Ha 2-e 3-14 cyTKH nocae 3apa?KeHnsi capKorma3ma pa36yx-
umx, C HepOBHbIMH KoHTypamn H BbI 51 14BaHHAMH, MbIIIIellHbIX BO-
JIOKOH CBeTIITC51 HHTeHCHBHO, HepasHomepHo B HHAe OTJIeJIbHbIX 3e-
peH, 111b160K, KOMKOB, cpparmeHToB pa3pymeHHoro MbILLIet1H01-0
BOJIOKHa, C 60J1bILIHM14 riposa.lamn 6e3 cHeileHHH.
04eBH,EIHO, B MbILIKAHOM BOJIOKHe 3apa?KeHHoro >1?(.. FIBOTH01-0,
nofweprHyTom AereHepaHHH, B capKorma3me npolicxo,IHT pa3pbH3
6eJIKOBbIX KOMIITIeKCOB H FIX pa3pymeHHe C BbICB060)KiJeHTIeM
MHO3HHa, K0TOpkIf1 fierqe coe,IHHHeTcq CO CBOHM aHTHTenom,
Hcae,acTHHe ttero npoucxo,aHT 6o,nee rpy6oe, qpKoe, Heparmomep-
Hoe cHeqeHne.
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TaHbl aHT1-Ipa61-IqecKHm 14 11pOTHBOCTOJI6HAIIHbIM aHTHTOKCHLIeCKHM
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pecueulla C HeJIb10 ho?ELTHeNK,aeHHH crieunclmqHocTH cHeqeHHH. HO-
Jo6Horo onucaHHomy, Ha3BaHHbIe momHHecuHpyiontHe rao6y.TH-Hibi B
MbILIIet1HbIX B0.110KHaX cHetieHHH He itaHam4.
48
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CIA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
IA-RDP80-00247A004300210001-9
1 Hcro,norntiecKoe, iHeloAHmypieurwe, 6noxnmHnecKoe H Hmmy-
H000rnmecKoe n3yneHne npn 110M011114 momnHecumpyrounix aHTHTeJI
mnorponnama Hnpycos KOKCaKH (113MeHeHHA B MbIthet1HOM BOAOK-
He) monceT aaTb u,eHHble cneaeHnn AAA nouumaHHn HeKOTOpbIX
CT0p0H 6140,1101'1411eCKHX CBOIACTB 3T1'IX Hnpycos.
HEKOTOPblE MIME 3AKOHOMEPHOCTH HOTPEEJIEH1451
KHCJIOPOAA XIS! BHPYCHO-BAKTEPHAJIbHbIX AXIC013611,140HHbIX
KOMIIJIEKCOB, CHCTEM (1)Af-EAKTEPH1I H BHPYC-KJIETKA
A. E. accefut
(CBepATIOBCKIIII1HCTHTyT Empyclibix mictreKutiA)
B011p0C 0 B3aHMOOTHOLUeHHHX Hnpycos H 6aKTepH14 KK
onpeaeaeHnon 6noneHoTHnecKoR cncTemm ao cnx flop He noarma
CKOJIbKO-HH6y/I,b yaon.rieTHopnTe.lbHoro peuieHHH, HecmoTpn Ha
6onbume o6Lue6Hoaornnecmoe 3HaiieHme 3TOH npo6nemm H HHTepec
ee jini HccaleaonaHnH npo6aem H4eKuHoHHofi naToaornH.
Pa6oTm JI. A. 3n.rib6epa n E. H. BocTpyxonon' (1933),
B. THmaKoHa (1936), n. F. oaTIbK0131411 H H. O. SIHymespni
(1936) noKa3aan, 4T0 Hupyc OCHOBaKHHHb1 MOH(140 Ky.113THBHp0-
BaTb Ha ,aponoRax H CT4IITIOKOKKe, tITO B cHm6Ho3e C 3THMH
mHKpoopraHHamamH, a TaK>Ke c capumHoil HHpyc He TOJIbK0 coxpa-
HgeTCR, HO PI paamHo)KaeTcs, H 11TO yKaaaHHbie B3aHMOOTHOIlleHHH
(B tlaCTHOCTII, rinpyc OCII0BaKHI1Hb1 H 'CTaCilI1J10K0KK) moryT HMeTb
mecTo B eCTeCTBeHHbIX ycaoHnnx.
B JurrepaType 011y6,1114K0BaHbI (H. F. Ceprnen, H. A. )teMHHa
H H. E. PHaaHnesa, 1945; I. K. FnmmeamPap6 c coTpy,aHHKamn,
1949; B. F. Bat1H6epr c coTpyanHKamH, 1949, 1951 H mHorne ap.)
maTepHaam, ocHemaioulne B031\40>KHOCT11 aa.cop6uHH Ellpycori Ha
pa3JIH4HbIX 63KTep143.11)HbIX B3BeCHX H HCH0J1630BaHne a11cop6a-
TOB B peaKLIHH arraionmaum4.
Cae,ayeT, KOHe4H0, HMeTb B HH,ay, Luro a,n.cop6u,4oHnbai Komn-
JleKC Hllpyc? 6awrepnfi OTH10,3,b He Hceraa monmo paccmaTpn-
BaTb KaK HetITO panHoueHHoe cmm6H0TnnecKomy Kommexcy.
ECTeCTBeHHO, 4T0 zum paaae,TienHn nucTo a,11C0p61.1110HHbIX H
CHM6H0TH4eCKHX Hnpycon-6awrepnaabHux accouHaunil 110.11)KHbI
6bITb Hbipa6oTaHm xaKHe-To xpwrepnH.
Ham npe,acTanafieTcn, Tro Hm6op HanpaHaeHlln nomcna TaKO-
BbIX ,a0J1}KeH onpeaeanTbcn camoti HpHpo,rloil OTHOLIIeHHil Bllpyca
H KTIeTKH-X03H14Ha B CBH314 C oTcyTcrmem aBTOHOMHOF0 o6meHa H
Hupa6oTxn atieprini y nepHoro (B. H. TOBapHIIII,KHA, 1960).
4 Te3liebl AOK.71a,a0B
49
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::',IA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
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ts3aHmoaeHcTsHe Blipyca H KzerKH, o6Hapy)KHBaemoe no 1.[H-
TorianiqecKomy, apclieKTy, sirmsnontemycsi KOHeT4HbIM BbipaweHHem
3TOTO B3aHmoJ1eficTrilisi, Ha Hain B3TVI5I,a, mo?Ker 6bITb BbISIBJIeHO
3HaTII4TeJIbH0 paHhine Ha OCHORe yea (11)H3HOJIOTH4eCKHX casHroB
(?cHrHa.rioB?) B cHcTeme BHpyc?KJIeTKa, rpy6bix islopcl)o.Tio-
THileCKHX 113MeHeHIA KJIeTKI4, 'coma Tpyailo oripe,a,enHTB, SIBJIRIOT-
CH JIB 9TH H3MeHeHHR o6meHa caeacTimem pa3mHwKeHHK Blipyca
HJIH nospeKrieHmi KJIeTKH.
Psz awropos . Bayep, 1956; P. H. arkiHro, 1960; E. ,a.14-
cpop,a, 1961) OTMeTlalOT, TO H3MeHeHHR B o6meHe yr.rieBoaos B
cHcTeme Blipyc?KJIeTKa, no cpaimeHino CHeHHCIMLIHp0BaHHOI4
? moryr 6bITb H3rieHm Ha cammx paHinix 3Tanax 3apaiKeHilli.
AHaJIOTHT1HbIe yKa3aHHB (3. B. Epmonbesa, 1939; A. E. 3cce.nb
H C. M. PaccmoB, 1957) Hmeiorcsi H B OTHOLLIeHHH 6awrepHocpara.
3a,Liaqy HacTonmero HCCJIe,a0BaIIHR COCTaBJI5IJI0 cpasHirreabHoe
H3rieHHe HHTeHCHBHOCTH noTpe6.KeHHB KHCJI0p0,21a 6awrepHaJlbHbl-
M11 B3BeC5IMH, aAcop6HporiaBLIDIMII Ha ce6e Bupyc OCHOBaKUHHbI,
B3BeCb10 6PIOLIMOTH4103HbIX 6aKTepHil HO,LI B03,LIefICTBHeM TOMOJIO-
THTIHOTO 6aKTepHocpara H KyabTypoil TKaHH Hep-2, 3apa}KeHHoil
a.u,eftormpycom 1-ro num.
IloTpeozeHHe KHcaopoiia onpe,rtexmocb maHomerpHtiecKHm
Me-
TOJWM B annapaTe Bap6ypra.
flpH H'CCJIe,ROBaHHH HHTeHCHBHOCTI4 norpe6.rieHHB KHCJI0p0,Ja
B3BeCb10 6PIOILIHOTH(1303HbIX 6awrepHil, Ha KOTOpbIX 6bui acop6H-
p0BaH BHpyc OCHOBaKUHHIDI, yCTaHOBJIeHO, TITO yAce Liepe3 30 MI111.
nocze a,LIC0p6III4H KOJIHT4eCTBO norpe6aBemoro KHCJI0p0aa pe3Ko
Bo3pacTaeT HO CpaBHeHHIO C HeHarpy)KeHHoR 6aKTepHaabHori
B3BeCbI0, H 3T0 pammilie CTaTHCT141.1eCKH AocrosepHo. Hmeer mec-
TO TaiuKe HapacTanHe noTpe6aeHHB KHCJIOOJ AJIR B3BeCH 6aK-
Harpy)KeHHBix HHaKTHBHp0BaHHbIM rthpycom. HHTeHCHB-
HOCTb norpe6.fleHHB Kftcaopoila.LJH1 B3BeCH 6p1OILIHOTH403HbIX 6aK-
Tepid"! ROA BonieficTimem ACHBOTO H HHaKTHBHp0BaHHOTO 6awreptio-
cpara Tax?Ke Bo3pacTaeT ripliqem 6o.nee 3HaTIHTeabH0 AJIR KOM614-
HaLIHH C }KIIBbIM 6aKTepHocliarom.
flpH 3apaA{eHHH B3BecH KileTOK Kyawrypu TKaHH Hep-2 azieHo-
Blipycom 1-ro THna (A. E. 3cceab, H. H. FJIHHCKHX H B. H. YCTbRH-
aen, 1963) ye qepe3 15 MilH. Houle BueceHHB BHpyca oTmetiaeTcsi
HapacTaHHe noTpe6.4eHHB KHcaopo,ita, npoito.maloweecsi B TegeHHe
7-8 qac. JLO momeHra ocso6o)KaeHHB BHPYC i13 KTIeTOK. TIOCJIe
3TOTO noTpe6oeHHe KIICTIOPOorla 3HaTIHTeabH0 cHH)KaeTcsi H npH6.in4-
31,1TeJlbH0 epea 15-20 qac. cosepuieHHo ripeKpaulaeTcB, Tora
KK B KOHTpOJIbH011 Ky.ribrype OHO eate ripo,rkomicaeTcsi HeKoTopoe
opemB.
HHaKTHBHp0BaHHbIll BHpyc He oKa3mBaeer CKOJIbKO-HH6yab
cyinecTBeHHoro BJIH5IHH5I Ha noTpe6.iiemie KHCJIOPO,Ita B3BeCbI0
KJIeTOK.
50
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TaKHm oopa3om, B npoBe,a.emibix HccaeuoBaHHHx yeTaHoBneHo,
qTO npH B03,aefiCTBH11 (B npeue.lax H36paHHbix mo,ue.nefl) BHpyca Ha
6aKTepHaabnyto B3BeCb, BHpyca Ha B3BeCb KJIeTOK KyabTypha TKa-
HH, a Taamce 6awrepHo4iara Ha 6aKTepHampHyro B3BeCb, noTpeoae-
HHe KHcaopoua Ho3pacTaeT.
YIIIITbIBa51, TO Hapacrame noTpeoaeHHH KHCJI0p0,11,a He HocHT
cneuHcpmecKoro xapaKTepa H HMT mecTo B caygasix aucop6uHH
HHaKTHBHposaimoro BHpyca HJIH 6aKTepHocpara, MWKHO npeunoaa-
raTb, !ITO MbI Hmeem ,ueao c o6LueR OTBeTHOIA peaKulleil KJIeTKH Ha
pa3upaweHHe, BbinaBaemoe aucop6uHeft Hccneuyembix cpaKTopos Ha
ee nosepxHocTH.
TutaTeabHoe KommecTBeHHoe H3ygeHHe npouecces ubixaHHH H
raHKomma B Komunexce c rHcTo- H 11,11TOXHMHIleCKHMH HccaeuoBa-
HilYIMH moiKeT onpe,ReJurrb BO3M0HcHOCTA pa3pa6OTKli KpwrepHeB.
caygailHocTH H 06JIHraTHOCTH BHpycHo-6aKTepHaabHbix accouHaumil
H HanpaBaeHHoro pauHoHaabHoro no,a6opa cHcTem BHpyc?KJIeTKa
,uHarHocTiviecmix H HCCJ1e,a0BaTeJIbCKHX
41143HOJIOTHA B3AHMOAEFICTB1151 ECHO-BHPYCOB
C BH0J101-144ECKHMH CYISCTPATAMH,
(1)143HOJI0rIPIECKA5I POJIb SH-rPYHH BHPyCOB
H. A. 3etim.4enom, O. H. Peti3un, B. M. Potixe.4b
IC M. rodabd0apr
(HHCTHTYT HOJIHOMHeHHTH H BllpycHbix 9HHecloHJIHTOB CCCP, MOCKIld
K HCJIY OCHOBHbIX cpyHKH,Hil noKoHineficff BHeKaeTotmoil cTa-
uHli pa3BHTH5 BHpycon?BHpocnopm (B. .TI. PbDKKOB, 1955 r.) ?
oTHoorrcH coxpaHeHHe >KH3HeCII0C06HOCTH BHpyca BO BHeKJIeT011HOCI
cpeue H BO3MMKHOCTH peaaH3aulin mexaHH3mop. HanaueHHH ero Ha
KJIeTKy?npHKpenaeHHB K KJIeTKe H BHeupeHHH B Hee.
)1?ItH ocyatecTmeHHH yKa3aHHbix >KH3HefillbIX cpyHKuHil BHpyca
cymecTBenHoe 3HatieHHe HmefoT xapaKTep H HHTHMHbIe mexaHH3mbi
B3aHMO,aerICTBHH BHpocnop c pa3np4HbimH 6110n0rligecKHmu cy6-
cTpaTamH. PacKpbITHe mexaHH3mort B3aHMO,LIefICTBHH BHpycoB c
6HamorHtiecKHmH cy6cTpaTamH TeCHO CBH3aHO C BbIHBJIeHHeM po.rm
6HonormtlecKH aKTHBHbIX XHMHT-IeCKHX rpynn 6eaKoBofi o6o.noliKH
BHpocnop. no HmeKnusmcH ,aaHHbIM (OHJIHIICOH H Llornum, 1960;
3etiTaeHoK H Pefi3HH, 1963), 6o.nbuloe 3HatieHHe BO B3aHmoueficT-
aeKoTopux BHpycos c 3pHTpou,wramH H IlYBCTBHTeJIbHbIMI4 KJIeT-
Kama HmeKrr CYJIbCPTHApHJIbHIDIe (SH-) rpynnbi.
B HacToRateci pa6oTe npeucTaBaeHm uanHble, noKa3bniaimuHe
pooh SH-rpynn BHpycos 7, 11, 12, 19 B npouecce B3aHmoilef1cTBHH
4* 51
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Declassified in Part - Sanitized Copy Approved for Release 2014/02/26 :
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,,Fr? puu,n I a. ivin ,leaubexa, tlyBcTBHTeabHumm KneTxamn myabTyphr
TKaHH nollex 06e3b2H, IIPOTe0JIHTWIeCKHM CPepmewrom (TpHncHH),
Hecneuwimitlecxilmn HHrH6HTopamH CbIBOpOTOK xpoBH ge.noBema
)KHBOTHbIX, KJIeTOTIHbIMH 3KcTpamTamH. HoKa3aHa TaiKKe .noxazH3a-
111151 HeCHeLIFHPHtleCKHX IIHTTI6HTOPOB FeMaITJIIOTHHHHOB BHpycoB
ECHO B pa3JIHLIFIbIX 6eJIKOBbIX 4:1)paKIAH51X CbIBOPOTKH KpOBH.
MepxanTHAoo6pa3yromme SH-pearewrm, p-mopmepxypHii6eH3o-
aT (PCMB) HgC12, HOJIHOCTb10 HHaKTHBHpyrOT remarrmoTHHHHEA
Hcc.neitoBaHHux BHpycos H CHH}KaIOT HX HHISPeKHEOHHy10 aKTHBHOCTb
B xyaBType TKaHH. HHaKTHBaLIHFI BHpyca o6paTHma no,a AefACTBHeM
BOCCTaHOBjleHHOF0 rayTaTHoHa Ham COJIHHOKHCJI0F0 LIHCTeHHa.
Am{HpylounIft SH-peareti?Ho,aaueTamHA?Heo6paTHmo
nO-
,LaBJIfleT reMarIVIIOTIIHHHbI Bupycos ECHO 11 H 19, He BJIHSBI B Tex
Nce ycoosmix Ha remarrmoTHHHHN BHpycos. ECHO 7 11 12, 'ITO yKa-
?3bIBaeT Ha painnime B TIOJI0)KeHHH H B (1JyHK11HOHaJIbH0f1 aKTHBHO-
CTH SH-rpyrinbl Ha noBepxHocTb tix BHpocnop.
TpHIICHH B xOHeHO1i KoHneHTpanun 0,25% He OKa3bI-
Baa BJIHHH1151 Ha remarrmoTHHaulloHHylo H HHCPentlIOHHy10 aKTHB-
HOCTb 11337IleHHbIX BHpycoB. 0,LIHaK0 Hoene npeaBappiTeabHoro 6.10-
impoBaHH5J SH-rpynn BHpycoB o6pa6oTxoil FIX PCMB yilaaoch no-
ma3aTB rJOJ1HO 4 Heo6paTHmoe noaaBaeHne remarrmoinHauHoHHO
aKTHBHOCTH H Heo6pammoe no,r(aBaeHHe HHCPeKLIH03H0-
CTH BHpycoB npH nocae,ayloakem Bon[eficTBHH TpHncHHa. 06pa6o-
TaHHbIe TaKHM 06pa3OM BHpycm He BoccTaHaBamBaaH cBoeil aKTHB-
HOCTII IIO,L( ,ReficTuHem pe,aynHpylonwx arellTOB (BGCCTaHOBJIeH-
HbIii rayTaTHoH). Henoiuoe nouameHHe HHcpexuno3HocTH ECHO-
BHpyca 7 noa BJIHAHHeM PCMB HJIH PCMB H TplincHHa 6bmo C1331-
311H0 C Hamnthem B BupycHon H0llyJI5ILI,1111 PCMB-tlyBCTBPITeabHbIX
(PCMB+) H PCMB-pe3HcTeHTHbix (PCMB-) BapHaHToB BHpyca.
PCMB- BHpocnopm He 6bIJIH CHOCO6HbI arrmoTHImpoBaTB apHTpouti-
TbI geaoBeica 0-rpyrinbi, He o6,71aAaJim BbICOKOA HHCPeKLIHOHHOcI ax-
THBHOeTb10.
MeToJom npenapaTHBHoro 3nexTpo4Jope3a Ha (PHJIbTp0BaJlb-
Hon 6ymare ycTaHowieno, tyro HecneumciwiecKHe FiHrH6HTopm rem-
arIVIIOTHHHHOB Bupyca ECHO 7 B cbmoporxax KpOBH ge.noBexa,
xpoJIHKa H JIOIlla,EFH JI0KaJIH33/10TC5I npenmymecTBeHHo B a0b6HMH-
HOBOti cppanuni; B CbIBOpOTKaX KpbIC B aabcpa- H 6eia-rao6yan-
Hax; B CbIBOpOTKaX 06e3b5iff ?B ramma- H 6eTa-rao6yaHHax. HpH
,FIRACTBIIH TpuncHHa Ha cbiBopoTKe xpoBH yTpagHBa.nach HX
FH6HTOpHaR aKTIIBHOCTb, FOB0pHT o 6eaxoBon ripmpo,Lce Hecne-
LIFICINVIeCKHX HHrH6HTopoB. 06HapyH(eno, o6pa6oTKa TpHIICH-
HOM HertTpanbHoro Komrmexca ?BHpyc ? HHI.146HT0p>> ne.na K pe-
axTHBaHHH remarrmormiaullommil aKTHBHocTll BilpycoB ECHO.
Ananornimbie aaHHbIe nanytieHbi npu H33,711eHHH B3allmooTH0weinift
CbIB0p0T011HbIX HFIF146HT0p0B C ruipycom rpm-ma (11y35111HHa C COT-
pymumamll, 1964). flo npe,rtBapuTeablibim ,LIaHHbIM, BO B3a14moaeti-
CTB1411 ECHO supycos c FiecneuminNecKnmH HHFH6HTOpaMH CH-
52
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Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
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BOpOTOK KpOBH ne.noBena ii -}KHBOTHbIX cymecTBeHHylo p0013 nrpa-
10T SH-rpynnbi Bupycos: Bnpycht, SH-rpyrinbi KOTOpbIX 6bIJIH 6.10-
KI-Ip0BaHb1 PCMB, He 110,LIaBJULTIHCb HecneumlnpieclinmH Hiir14614TO-
pamn.
CaneBoii ancTpanT TKaIlll no'-len 06e3b9H ona3a.ncn aKTHBHbIM
Hur1-I6wropom remarroloTHHHHoB Bnpycos ECHO 7 H 19. HHrH6H-
pyloutnil cpaKTop 3KcTpanTa TKBHVI He ,aeficTBoBaa Ha BHpycm, o6-
pa6oTaHHme PCMB, 'cm roBopwr o Banmon po.rin B 3TOM itieHo-
meHe SH-rpyrin Bnpycori.
Pe3y,IIbTaTbI ITCCJle,LIOB311115I II0KB3bIBalOT BayAHoe 3Ha4eHne CB0-
60HbIX SH-rpynn Blipocnop B npoueccax B3314MOJefiCTBHH my-
IleHHbIX ECHO-BupycoB C pa3,1114,-MbIMH 6HO,TIOFHIleCKHMII cy6cTpa-
SH-rpynnm Bnpocnop He06X0,1,1-1MbI J1JI11 ocyulecTthriembi B3B11-
MO,RefICTBI-IFI ECHO ? Bnpycos 7, 11, 12, 19 C IlyBCTBHTe.TIbHbIMH
KfieTKamH noneK 06e3b5111, C 3p1-ITpoukrramm geoorsena 0-rpynnm, C
CbIB0p0T011HbIMH H TKaHeBbIMH HHrH6HTopamn remarroloTHHHHoB.
C zi?pyron CT0p0HbI, SH-rpynnbi 3aumilatoT BHpocnopm yKa3aH-
HbIX Bllpycos OT ry6HTeaBHoro B03,1Ie1ICTBHH TpuncHua, wro, B03-
MO>KHO, HMT 3Ha4eHHe itasi coxpaHemin NneHne SH-rpynn Ha o6o.rionxe Bnpocnop H 14x
3HanellHe B npoueccax B3BHM0efICTBFI11 C tlyBCTBHTeJlbHbIMH KfleT-
Kamii H 11p0TeaTIFITHtleCKHMH cpepmeHTamil pairanino y pa311bIX
BHpycos H ,TI,a)Ke y reneTkinecKH pa3HB1x BapnawroB 0,II,H0r0 H TO-
to ice Bnpyca.
HEKOTOPblE )1,AHHblE 0 11P14P0AE WTAMMA BHPYCA ?BAKb1-7c,
Bbl,B,EJIEHHOTO 143 Cb1BOPOTKI4 B0J1bHbIX
HH4'EK14140HHUM rEnATHTom
K. I'. Kepum3aae, JI. H. A.4eunepoket
(HarlHO-HCCJleA0BaTeJlbCKlifl HHCTIITyT 3M.Hr, Baxy)
LLITamm ?Banbi-7c? npomen 13 riaccaNiefl. clommmo Hero, 113
CbIBOpOTKH 60,TIbHbIX HHCkeKII,HOHHbIM renaTHTom BBLIeneHo eme
3 LuTamma, Ho nepBbal 113yneH 6thnee nozipoono
DTOT urramm HHTepecell H noTomy, 4T0JaeT B 3Ha4VITeJMHOM
no.ruinecTse caynaes n000)BHTe.ribuyio peaKuwo npeumnuTaunn c
cbmopoTnamn 60.1IbHbIX H cneumpHnecKoe cBeneune npH BOCTBHOB-
Ke OrIbITOB Henpnmmm meToaom KyHca.
BBLria,n3ymeHa BO3M0>Klia2 po.rth, meToilHoro HmmyHHTeTa naK
np11 11HHbl TpyB,Hoil BBIB,e,TmemocTH Bnpyca H114)eKU,HOHHOI-0
53
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renaTHTa. B HaCTHOCTH, 6bITI0 o6HappHeHo, HTO ripH 3apa)HeHHH
1Ipo6HpoH C 6OJIBIIIHM KOJIHtleCTBOM HenpeuHriaembrx KJIeTOK noireK
qe,noueHa mTammom ?BaHur-7c?, Hapqay c pacnaHruHmHcH, pa3-
pyweHHhimH KJIeTKaMH, HMe,TIFICb H rpynnu HaeroH, JI0 imity co-
BepweHHo 3,a0p0BIDIX. HeCMOTpH Ha TO, -ITO noc.ne TWaTeJlbHOTO
BCTpHXHBaHHH H IIp0MblBaHH5I pacTHopom XeHHca ocTanumecH mJemie 60.T1e3HH. flepubdi
143 HHX, Hmeioumn HaH6onbuiee "-MUM CTOpOHHHKOB 0 ckylnumo-
HaJIbHOM xapauTepe 3a6oaesaHHH, OCHOBaH Ha TOM, IITO 60J1e3Hb
B paHHefi CTa,a14H H3.ne4m3aeTcH nyTem ,T1,0110JI1-IHTeJlbHbIX noHop-
MOK aepesbes HeKOTOpb1MH M14Kpo3J1emewramll (U,HHK, )Ke.ne3o) B
coHeTaHHH C THIATeabHbIM co6moueHHem Beer? KOMnaexca oftue-
arpoTexmmecimx meporipHHTH11. BTopotl? 0 HHCPeK11110HH0c1 npn-
poae ? 6a3HpyeTc11 Ha yHenntumalomemcH c uaKTI,bim ro,aom cimK-
THHecHom maTepHane o unpycHom npoHcxoKqemm 3a6o.rieuaHHH.
Ha6J1IoJaembie aBTOpaMH B yCJI0I3 HHX YHpaimm np113Haim 6o-
B OCHOBHOM COOTBeTCTBylOT HmeimuumcH B AHTepaType 01111-
caHil5im. 0,rmalco HMH Heo,TwoxpaTHo oTmeim.nacb 0,4aFOBOCTb B
pacnpocTpaHewm 60J1e3HH, npoganeHne ee IlpH311aKOB Ha 3,110p0BbIX
B3pOCJIbIX aepeBbsix, eCJIH OHM paCTT BOJIH3H OT 60JIbHbIX 14 no-
pa?KeHne no6eros, pacTyumx B6JIH3H c.rieJou o6pe3HH BeTOK. Bo-
dle311b B IIHTOMHHKe moxceT nopaHcaTb OT,ReJIbHbIe ca?KeHubi, pacTy-
Lune pwaom C 13 110JIHe 3,apOBbIMH. TaKHe OCO6eHHOCTH 06b1 6bI-
BaRyr xapaKTepubi iirii HH4)eKLI,H0HHbIX 6o.ne3Heci.
BbISICHeHHH nplipoabt 3a6o.rieBaHH5l B 1962-1964 FF. npo-
Be,rkeHm pH,Ronbyrou H.ria6opaTopmpix Hccae,a,oBaHHH B Hanpasaemni
Bb15ICHeHHH BO3MO}KHOCTH 3apaHcemm HAM, BepHee, nepeu,atm 6o.ne3-
H1'I COKOM 60J1bHbIX JI1ICTbeB. B KaHecTHe 14CTO4HHKa 11 111:1)eKIIHH
6paanch 133pocabie aepeubH, C KOTOpbIX co6HpaJm JI11CTb1 C cumn-
Tomamn 60JIe31-1H, a ;Lim KOHTpOJIH ?JIHCTbH 6e3 cHmnTomou H J1 14-
CTbYI 3a0p0BbIX Aepeubes. ,LI,J151 3apa?KeHHH HCII0J1b30BaJIH AByx-
JIETHHe ca?KeHum H 0110J1eTHHe CCHHUbI H6JI0H14 H rpyum, a TaK-
AKe pacTeHHH ['up-um, ayILIFICTOF0 Ta6aKa H TOMaTOB. B pa6oTe
IICHOJIb30BaJIHCb 3KeTpaKTbI 3THX JIHCTbeB nocae (1)HabTpau,HH He-
pe3 BaTHHR HJIII ac6ecT0Bbal 4m,r1bTpu, a TaloKe CkHOTIbHbIe
3KCTpaKTbI HyKj1e11110BbIX KHCJIOT. 3apaNcelme npop.oxlim FIJIH
nyTem HaTnpaHHH 011bIJIeHHbIX Kap6opyHaom J1HCTbeB, HJIFT ripm
HOM011114 uaymyKoHort Tpy6xu, 113 KoTopon HH(1)eKUHOHHaH >KHAKOCTb
13CaCblBaJlach HHyTpb cpe3aHHoro II0,a 3TO 11 >Ke }1{14,11,KOCTb10 Oa10-
.neTHero no6era.
01.1bITbI 1962 r. Jam{ 110J10}1{HTeJlbHbIe pe3yabTaTu. Bo Hcex
cayHanx 3apa)KeHHH caxi.enues 3KCTpaKTOM 113 JIIICTbeB C CHMIITO-
p03eT0IIHOCTH, B ,II,1CTI4JIJIHTe 11.1111 B (1)0C(PaTH0M 6ycpepe,
lip0HBHJIHCb 11pH3HaK14 60J1e3HH. B 1964 F. HeKoTopme 143 3THX Je-
pesbes B CHJIbH011 cTeneHH oxuageHui 3a6oneualnlem.
flo6erH c npH3Hauamn 60.11e3H14 HmeloT 3H3LIHTeTlbH0 meHbLunil
npupocT (Ha 56%) n HaMH0F0 60.Thee HHTelICHBHO BeTBHTCH.
3apax?ceHHH, npoueJewime 13 1963 F. B no.neumx ycnounfix,
143-3a He6.naronpH5ITHbix JAR pocTa ,aepee B noroAbi He MOF.T1 11
6b1J1 14 6bITb yHTeHbi. B yCJI013115IX HKe TeLIJIHI1b1 6b1.11a nallyHeHa
nepeAaim 6one3Hu so ucex c.xlytiaHx 3apa}Hemm caxceHueu rpyum
3ucTpaicram11 JIHCTbeB 516.110H11 C cHmnTomamH 60J1e3HI4 14 He 6bma
61
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.";1A-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
1A-RDP80-00247A004300210001-9
HoriyHeHa HH B OAHOM cayHae HCH0J11330BaHH5! micTbes 3Aoposbix
Aepesbes.
B aoKaaile ocseakatoTcH Tame pe3yabTaTbi ,a,pyria OrIbITOB no
nepeaatie p03eTOHHOCTH, B TOM Hucne 3apaeHHem cesulltes H6-
J1OHH H rpynni, a Tame 0,aHOJIeTHHX pacTeHHfi HaTHpaHHem H 3a-
pax(eHHH caweHuei3 5I6JIOHH 01PeL10.1113HUMH axcTpawramll Hymen-
HOBbIX KHCJIOT.
Pe3y.11bTaTbI 3J1eKTp0HHOMHKpOCKOITH4eCKOTO HCCJIe,a0BaHH51 HC-
110J1b3yeMbIX B ormaTax 3KCTpaKTOB noKa3aJui HaJTHHHe B HHcpentH-
OHHbIX }KHAKOCTSIX 011eHb peaHo BcTpeHarow,HxcH 11&110i1KOBH,L1HbIX
HaCTIllt 1JIHH010 150-800 iuc, c HaH5o.nee HacTo HcTpeHaiontHmcH
pa3mepom 600 .4i.,14K. HOCKOJIbKy TaxHe cTp3ncryphi He 6bIJIH oTme-
geHbI B npenapaTax 113 3KCTpaKTOB 31Wp0BbIX JIHCTbeB, aBTOpbI no-
aaraloT BO3M0H{Hb1M, HTO OHM MOryT HMeTb oTHomeHme K H035y-
AHTemo 60,11.e3HH.
B HTore npoBeAeHHoil pa6oTbi arsTopm IlpHX0,LUIT K BbIBO,ay 0
1411(1)eKIIHOHHOCTH p03eT014HOCTII 516J10H14 Ha YmpallHe.
HHCHBHTOPM OHTOHATOITHHbIX BHPYCOB
H HYTH HX HCH0J1b3OBAHHA
A. A. Bo6bipb
(1411CTI{TyT MHKPO6HOJIOCHH atipyconorim HIC A. K. 3a6onoTHoro AH YCCP)
TOKCINHOCTb HHrH6HT0p0B HHpycos JOH paCTeHH5l-X03HHHa H 06-
paTHMOCTb Hbi3bnmemoh HMH IiHaKTHBaHHH HHpyca ? OCHOBH0e npe-
BFITCTBHe Ha nyTH HX HCH0J1b30BaH1151.
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AHameTpaahHo HpOTHBOHOJIWKHO CB.513aHb1 C HoHuellTpaumeft Heim-
CTBB H Hp0,1240J1}KHTeJ1bHOCTb10 ero Ho3,aeficTHHH Ha Hilpyc H pacTe-
HHe. 3T11 cHoticTsa 3aBHCHT B 3HaLIHTe.11bHa cTeneHH TaioKe OT BH-
,a0B011 H copTosoil CrlegH(1)14K11 pacTeHHH-xo35lHHa H pa3BHTHSI B Hem
HllpycHoil HHcpeKHHH.
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Ha FIX TOKCHI1HOCTb H 06paTHM0CTb Bb13bIBaeMOR HMH HHaKTHB.31.11114,
oTHpbn3aeT BO3M0>KHOCTH HCII0J1b30BaHHH ,11,1151 XHMHOI1p0C1HJIaKTHKII
aHTHBHpyCHbIX HemecTH c HecnermimHecxHm AeficTHHem.
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6HT0p0B EnpycoB ,a.1151 XHM11011p0C1/14J1aKTHKH H Tepanllm C 1.1.111p0KHM
CrIeKTp0M aHTHHHpycHoro AeAcTHHH?flyTb K HOBbILLIeHHIO 30tex-
THBHOCTH aHTHHHpycHbix HeulecTH.
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
BJIHRHHE BHEI111114X YCJI0B14171
143MEHIIHBOCTb 1311PYCOB
H3VIEHHE HHAKTHBHPY101HET0
H MYTATEHHOTO ,B,EFICTBH51 rHAPOKCHIIAMHHA
HA BHPYC HOJIHOMHEJIHTA
10. 3. fegdou
(MocxoBcimil Haymito-HccneilowaTeAbcKult 1111CTIITYT 1311PyCHbIX ripenapaToB)
1. B nocae,n,Hee HpemH TIOHBHJIHCb pa6oTbi B KOTOpbIX onucano
myTareHHoe xteticTillie rwapoxcH.riamHHa B onbyrax c 6awrepHocpa-
ramH, ruipycom Ta6a4HOR MO3aHKIT H Hllpycom 60.TIe3H11 HbIOK3CT1I6.
Pa60TaMI4 IllyeTepa H JP. ouzo noxa3aHo, TITO rwapoxamamini pe-
arHpyeT npeHmythiecTseHHo C HilpHMHAHHOBbIMI4 OCHOBaHHHMH Hy-
KVIel4HOBOil KHCJI0TbI, ripmem B c.ria6oHlic.nal cpetke npoucxo,RHT 113-
MeHeHHe mainibim o6pa3om LITITO3HHa, a B HIRJI011H0f1?ypallnaa.
B HacToHateci pa6oTe H3y11eHo ithaxTHHHpylomee H MyTareHHoe Aert-
crime rwapoxcH?TiamHHa Ha Hupyc TIOJIHOMHeJIHTa npn 06pa6OTKe
HaTHBHOTO BHeKTIeT011HOTO Hiwyca H HllpycHoil PHK in vitro.
2. H3y4eHHe imaxTHEHippoulero aeficTHHH rH,RpoxcH.namiiHa npH
pH 7,1 noKa3a.rio, TO HHaKTuBaUH1 HaTHHHoro Hllpyca, KK H 1311-
pycHoil PHK, IIp0HCX0,21,HT,.KaK peantHR nepHoro nopH,axa. KaicHx-
JI II 60 pa3JIIVIHR B CKOpOCTII 11.1114 xapaxTepe xpimoci HHaKTHBaHHH
npH ripose,u,eHHH onbyra B H3OTOHHeCKOi 11.1111 rHriepTolunrecxoff
cpe,ile He 06HappICHBaJI0Cb. KHHeTHKa CH11)KeH115I 11114eKII,1OHHOCTII
HaTHBHOTO Hiwyca H ruipycHoik PHK 6buta. npaimmecHH 0,11,HHaKO-
Ba. 143 npenapaTos HaTHHHoro Htipyca, HHaKTHBHPOBaHHOTO THap0K-
CHJIaMTIHOM, He y,EkaHa.nocb BbIaeJIHTb 14114JeKUHOHHOCI PHK. Bony-
HeHHbie ,itaHHble CBHAeTeJlbCTBylOT 0 TOM, TITO HHaKTI4BaH14YI mipy-
ca no.riHomileawra rnitpoxcHaammHom HH TICHOJIb30BaHHbIX ycno-
B11RX OTIbITa Hp0HCX0,11,HT F.TIaBHbIM o6pa3om 3a ctieT pa3pyineHHH
HyKJIeHHOBOTO KomnoHeHTa rinpyca, a He ero 6eJIKOBOcI
DTH pe3y.ribTaTbi TIO3BOJI1IOT BbICKa3aTb npe,arionox(eHHe o TOM,
ritapOKCH.Tlammi mox(eT 6bITb C ycnexom HCHOJIb30BaH JJ15T
npHroTomeHHH BbICOKOHMMyHOTeHHbIX HHaKTHBHp0BaHHbIX Hiwyc-
HbIX BaKHHH.
3. H3pieHHe HHaKTHHHpyloutero ,a,eficTHHH rH,u,poxcHaamHHa Ha
HaTHBHbal Hiwyc TIOJIHOMHeJIHTa H illipycHylo PHK rim{ pH 6,1
63
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Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
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, pActvicnne LUIT031411a) H npH pH 9,1 (npeHmy-
inecTneHHoe yaa.rieHHe ypaltHaa) noxa3a.rio, 4T0 H114/eKUH0HH0CTb
PHK pa3pytnaeTcH npH pH 6,1 3HaLIHTeJ1bH0 6bicTpee, gem npa
pH 9,1. B TO }xe spew! B 011bITaX C HaTIIBHhIM BHpycom noTepg
Hliq3eKL1HOHH0CTI4 npH pH 6,1 ripollexoxIna 3HaIIHTeJ1bH0 mea.rieH-
Hee, tiem npH pH 9,1. KLIHTbIBaH, CHH}KeHHe Hilq)eKL1H0HH0CTH
Bapyca HOJIHOMHeJIHTa npH ,L1efICTBHH FH,L1p0KCIIJIaMHHa CB513aHO C
npeHmymecTneHHum pa3pymeHHem nripycHoCi PHK, moxmo noaa-
raTb, LITO B 1.1.1e.110HHOff cpejle HaCTINHO HapymaercH LjeJIOCTHOCTb
HupycHon 6efixonoil o6o.notncH H o6.ner4aeTc5i npoliHxHoseHne FH,/j-
policitnamaaa K HyxoeHHosomy KOM1-1011e11Ty nHpyca. KOCBeHHIDIM
no,ITnepKri,eHHem 3T0 TO'IKH 3peHHH cay>maT rioaygeHHbie Hamll
AaHHIDIe 1133PleHHH ctioTozwHamtigecxoro aeficTnHn npocimanHHa Ha
nHpyc 110.TIHOMHeJIIITa. B 3THX oribiTax 6bulo noxa3aHo, T.ITO noTe-
P51 HHCPeK1.11,10HHOCTH npI4 1:POTOAHHam11tiecKom ikericTi3H11 111)04:ma-
? Ha 13Hpyc HOJIHOMIleJIHTa o6ycnomeHa pa3pyineHHem Hyx.riell-
HOBOr0 KOMII0HellTa impyca. Tax, ripH pH 7,1 HaTHBHb111 niwyc He
HHaKTHBHp0BaJICH ripH (pOTOAHHaMllgeCKOM AefiCTBHH ripotimamina,
B TO npemn KaK PHK 6bICTp0 TepHaa HHci)exnuoHHocTb. 0,aHaK0
11pH npoi3e,aeHHH 01113ITa B rue.mogHoTt cpeae HaTHBHbIfi impyc Tax-
CHH>KaJI HHCl/eKL1HOHHOCTb npH (11)0TO,E(HHaMHHeCKOM AefICTBH11
npocp.namma.
4. PhytteHHe myTareHnoro ,111efiCTBHH rwapoxcimamHHa Ha BH-
pyc narmomnemyra rip ii pH 7,1 noxa3a.mo B0CIW0H3BO4HM0e H cTa-
THCTIFIeCKII ,L1ocTosepHoe BO3HHKHOBeHl1e myTauHfi HO S-, d- H
TC140-1-1pH3HaKaM [IH o6pa6oTxe HaTHBHOTO BHpyca H 13Hpycnoti
PHK in vitro. 011aK0 IlacToTa myTattHil B 01IbITaX C BHpycHoii
PHK oxa3a.nacb nplimepHo B Ana pa3a BbILLIe, Hexce.HH npH o6pa-
6oTme HaTHBHOPO inipyca. B 011bITaX C BHpycHoil PHK o6Hapy)KH-
Ha.r1acb onpeiterteHHaH CrIe11H(P/111HOCTb myTareHnoro ,L1etICTBFIH
rn,apoxcHaamHHa, Bbipamiainnancsi B ripenmyntecTneHHom H3meHe-
H1,114 S-ripH3Haxa 13Hpyca. Kpome Toro, rHapoxcH.TiamHH BbI3bIBaJI
BO3HHKHOBeHile myTaHHA TO1113K0 npn dReilcTrinu Ha PHK, BbI,aeJleH-
H),10 143 BHpyJIeHTHIDIX ILITaMMOB Bapyca TIOJIHOMlle.naTa (npsimbie
myTaunH), HO He Ha PHK, nonygeHHylo 113 aTTeHyHponaHHbix inTam-
MOB (o6paTHme myraHHH). B pn,ae OIIIDITOB C PHK, BbI,JeJleHHOfi 113
Einpy,rieHTHbuc 1.11TaMMOB impyca H0JIHOMHeJ111Ta, yamocb 1-10JITIHT13
myTaHTm, o6aanatoutHe CBOIACTBaMH BaKI.114HHbIX HiTammos, BKJI10-
ma51 anaToreHHocTb ATM lipllMaTOB.
5. 1/13ygeHHe myTaretworo ,aeficTnpin rH,IpoxclinamHHa Ha PHK
B cAa6oxncaon HJIH Ige.11011H0f1 cpeike noxa3aao, LITO npH pH 9,1
mmeer mecTo HOCTOHHHOe BO3HHKHOBeIlHe myTaHHA no S-ripH3Haxy,
B TO BpemH, KK ripm pH 6,1 110HBJIeHHe MyTaL11111 lipaKTHT-leCKH OT-
cyTcraosaao. Y1114TbIBaH nwirieHHbie aaHHbIe, MWKHO noaaraTb, HTO
BH ,aeficTi3HH ru,apoxcimamHHa Ha BHpyc nonHomHeallTa BO3HHKHO-
Beane myTauHci no S-npH3Haxy CBH3aHO C 143MeHeHHHMH B cocrane
ypaulma, a noTepn HHCPeKLIHOHHOCTH 06yCJIOBJIHBaeTCH y,la.nerthem
64
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Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
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U1T03111-Ja. YgHTWBaA onpeAeaennym CHeH4H4HOCTb myTarennoro
AencTimin rnapommamnna H BO3MO}KHOCTb noaynennn MyTaHTOB
CO CBOACTBaMH BaKUHHHMX nnpycntax IUTaMMOB, MONKHO noaa-
ran), 4T0 3TOT myTaren nanAeT tunpoKoe npnmenenne Ann pewe-
H1451 pnAa npalowlecKnx H TeopeTnnecKnx nonpocon Bnpycoaornn.
AEGICTBHE 5-5POMYPAHHJIA H 5-4)TOPOYPAHHJIA
HA HILRYKKHIO BHPYCA J1H30rEHH00O3
atiwEHHoro THMHHA MYTAHTA E. COLI
nPti YJIbTPA41140JIETOBOM CBETE
X. A. Po3eitmailb
(4HCINTyT Buipycoaormm, Beivinn)
ECJIH 6pomypau,Ha HaeagH HmecTo THMHHa B JthaoreHHoil
6awrepHH, TO KJIeTKIT 'WM:IBM:HOT yae.riHT-ieHHylo tlyBCTBVITeJIbHOCTb
K yobTpackHoneTosum H3JIygeHHAM H no6yKaa1orrcH K CHHTe3y BH-
pyca npH 3Ha4liTeJ1bH0 60JIee HH3KHX ,aoaax. Eciii oxiospememio
B PHK JIH30FeHHOct 6aHTepHH nplicyrcrayer clyropoypauFlo, KJIeT-
K14 HHaKTFIBlipylOTCH yAbTpaCtiliOneTOBbIM CBeTOM, a He B036y)K,Ra-
10TCH.
DTH pe3yabTaTm TIOKa3bIBalOT, gTO nepnwinue mari nnpycnoA
nuAyKunn C HOMOILLIDIO yabTpagnimeToHoro cHeTa BbI3b1BalOT peaH-
11,1411 y maneHyn ,LUiK KJIeT0,1HbIX xpomoaom H, HepoHTHO, PICKJI10-
4a1OT peaHuHH, HacalowHecH camoro HurH6wropa H mcoeHyn ?E1-1K
aHpyca (npocpara), pery,THipyH CHHTe3 H aefICTBHe HHrH6HTopa.
K BOHPOCY PEAKTHBAHHH BHPYCA SIW,YPA
H POJIH BEJIKOBOVI 060JI0LIKH B 3TOM IIPOHECCE
E.EHu,um B,JL CAVAN?
(14HCTHTyT Bei-emu-lam-mg impycoaormi, Mocium)
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TN peatcrnnaunn nnpycon ocnu B oprann3me >KHBOTHOF0 npnoAno-
npemennom BBCaeHHH HHaKTHT1Hp0BaHHOCO H NKHBOr0 nmpycoB.
B HacTostwee spew' B cneTe OTKIMITHR reneTnnecKoR poan nyKae-
nnonmx KHCAOT mexamum 3T01'0 cloenomena CBA3bIBatOT C coxpane-
}mem TpaHcckopmHpylowero cloawropa?,IIHK.
ClAnaKo no pealunnaunn HHaKTHBHp0BaHHWX nnpycon B ycao-
5 Te3HCW AOKAaAOH
65
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Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
,IA-RDP80-00247A004300210001-9
cHcTembi HmeeTen cpasHHTempHo ma.no itaKTFItleCKIIX
B 1954 r. HopTon Ha6mo4a.1 peaKTHBanmo IlHaKTHBHP0-
BaHH0F0 cpara nyTem cycneHallponaHnn ero B xoHneHTpHporsaHHux
pacTsopax aueTaTa HaTPHH HJIH B caerKa KIICablX pacTsopax nenTo-
Ha. HmeeTc51 p5T4 coo6meunin 0TH0CHTeJlbH0 peaKTHBauHH mHxpo-
0prall143M0B H cparon, 06.nytieHHb1x ymapael)HaneTonumH ayqamm
(?TlaTap?Ke c coTpy,anHxamH, 1954; ,Il1an6eKKo, 1949, 1950; Ke.nh-
nep, 1951; K3m6eaa, 1950; Bapa6oil, 1961).
B HacToniftem coo6meHHH HamH IIPHBOASITCH axcnepHmeHTaab-
Hble ,aamibie no peaKTHBauHH 8Hpyca Hatypa, IlHaKTHBIlp0BaHH0F0
FJIy60KHM 06e3BWKHBaHHeM, KHCJI0c1 Cpe4OCI H yabTpacimaneTonbi-
M14 .nyqamm BH e ?pram-lama )1{1-IBOTHUX.
B 011bITaX HCHOJIb30BaJIH JIaIIHHH3Hp0BaHfIbIR BHpyc Hulypa THna
A. Fay6oKoro 06e3B0>K11BaHHH sHpyca 40 ocTaToqHoci BJIa}KHOCTH
0,2-0,4% ,aocTHralm shicyumnaHHem B crienHa.nbuoil cpe4e. MHaK-
711BaIIHIO BHpyca B KHCJIOCI cpeae OCylHeCTBJIHJIH, 406aBJIHH K sHpy-
cocaaep)Kameil cycneHanli pacTnop cans:moil KIICJI0TbI 40 pH 5,0 H
nbmer*Knnan ee B TemHoTe B TegeHme 1 qaca. Y.nbTpact,HameTonoe
06,713/geF11-ie .namnoci BYB-30 B 4o3e 8000 ape/cm2 06ecneqm3a,no CHH-
)Kenne aKTHBHOCTH BHpyca B BbICyaleHHOM COCTOHHIIH Ha 2,5 ?
3 .norapHcPma.
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BIDIC0K011 TemnepaTypok H YJIbTPa3ByK0M H nomentaaH B Hale
yJIbTpaBbICOKOA naCTOTbr (YBLI). PealcrHaanwo sHpyca, HHaKTIIBII-
P0BaHHOF0 B KIICJI011 cpe,ae, 11p0H3B0411JIH 4Byxpambim noxneaaqH-
BaHnem cpeAbi 40 pH 9,5, yabTpa3Byxon B ,aoae 6.101' ape H B nane
y.nurpanbicoxoci HaCTOTbI. BHpyc, HaCTHT-IHO 14HaKTFIBIlp0BaHlIbIll
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BJIa}KHOCTH 0,2-0,4% aKTHBHOCTI3 ero cHH)maeTcH B 1 000 ?
1 0 000 pa 3 cpasHernuo C supycom, BbICYIIIeHHbIM 4,0 BJIa}KHOCTH
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BaHHem 40 80?, FIOJIHOCTIDIO HHaKTHBIIPOBaJICH B Teqemie 30 MHH. B
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BHpyc, peaKTHBHponaHHE4 ,apyrHmH meTaaamH, oxaamen yeT011LIH-
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BaJ111 CliopmupoBanie aHTHremarrmoTHHHHos H pa3Jumbix BH,TOB
allpycHefiTpanH3yrom1x aHTHTe,TI y .ma6opaTopHbix >KHBOTHbIX H y
11\imyHoreHHa5i aKTHBHOCTb HHaKTHampoaaHHoro sapyca coxpa-
nReTC51 T0,71bK0 B Tex C,TIYHaHX, Kora B CYCHOH3HH Haxo,a,HTca CBO.
60,1Hbffi ckopmanHH. Flocae HefrrpaaH3auHa .1:131opmaAHHa 6acy,lbc1w,
TOM BaKLUMbl TePSIJIH CHM aKTHBHOCTb B TegeHme 1-2 mecsmeB,
110A B,TIHHHHeM ckopmaoHHa npollcxo,anT o6pa3oBaHHe Komi-1,1ex,
COB impyca 'C TKaHeBbIN1H dwarmeHTamH. DTH KOMILTIeKCbl
B OCaa0K no,a. B,IHRHHeM HpoTamHHcyabckaTa.
Bnn pa3pymaeT ace CBOACTBa Bilpyca. 0,1Hako CKOPOCTb HHaK-
THBaHHH HeoxiHaxosa: cHatiaaa pa3pyumeTcg HHCDeKHHOHHOCTb,
3aTem HmmylloreHHacTb, crioco6HocTb arrmoTHHHposaTb 3pHTpoua-
TbI H, 'HaKoHeu, KomimemeHTeasubmaiontaq aKTHBHOCTb.
BllabHOM abi6ope O3bI HpenapaTa H TemnepaTypHbix ycaormi1,
C OHOH CTOp0Hbl, H caoespemenHom npekpauteHHH AeficTaHR
Bnn, c Apyroct, MO}KHO narlytiaTb HeHH(PeKHHOHHble ripenapaTbr,
o6na,aaroLuHe Bcemll HHTepecylow,Hmu amcnepHmeHTaTopa CBOrl-
cTsamll. HmmyHoreHHaa aKTHBHOCTb HHaKTHBHp0BaHHbIX Bull cyc-
rieH3Hci BHpyca pa3pymaeTcH B TegeHHe 2 mecalleb. B npncyTcraHH
npoTamHHcyabckaTa ace cHoficTaa HHaKTHBHp0BaHliblX npenapaTos,,
B TOM 4Hcae H HmmyHoreHHocTb, OCTalOTCCI 6e3 H3mellefifici Ha npo-
T2>KeHHH
EIJIACTIPIECKHE CBORCTBA MEHIAAHUIHX OPrAHH3MOB
HA HPHMEPE BHPYCA SHHYPA
E. B. AkOssLo, H. E. TOACMR1C, M. ,a. Ecticymellmo
(Ygpamictilla Haymtio-Hcc.neAoBareobothil 11HCTI4TYT 3KCITepliMeHTaJ1bHOft BeTepitHapim)
LiaBHO H3BeCTHO, HTO HIHTeHCHBHOCTb pa3mHo>beHHH allpycoa
3aB14C111 OT ItH3HoolorHgecxoro COCTORHHA opraHR3ma H TecHo CBH-
3aHa co crpyxTyporf H 061I4M meTa6oimmom IK.TIeTOK.
HaLua Hecae,aosaHHH noxa3mH, TaKHe 6H0a0rmeckHe caoh-
.craa aHpyca Hwypa, xax HMMYHOreHHOCTb H aliTHreithocTb, 3aBHCHT
He TO,I1bK0 OT ypoaHR OKIICJIHTeabH0-BOCCTaHOBHTeabHbIX ripoueccori
B KaeTxax, 'HO H OT BH,Ta TKaHH, B K.rfeTKaX xoTopog pa3mHo>KaeTc5
bmpyc.
.68
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
CIA-RDP80-00247A004300210001-9
CIA-Declassd in Part - Sanitized Copy Approved for Release 2014/02/26:
RDP80-00247A004300210001-9
Y Tpex unammos Bapyk_a Ir11LJD (mamma ?TanaecKalb> a
?Bo6pcKA?) H A (urramm ?Cep6ixeci) npaxTrniecxli He H3MeHHJIH HameucTuernibtx
cHoileTH Hilpyca, 3a Hcx.rnotierniem rct25-npH3Haxa: nocne HSITH nac-
ca)xect impyc ripHo6pe.ri cnoco6HocTb pa3MHO)KaTbC51 npH 25?.
1/13meHeHH5-1 Hacae,acTHernibtx CBOACTS y H3ygeHHbix ulTammou B
npouecce nacca?Keil npH 250 oxa3aoHcb Heo,3,HHaxosumH. Tax, nac-
caNth npu 250 ll1TMM Mahoney npHHe.nri K H3meHeluno mHorrix
reHeTHmecruix IlpH3HaKOB, xapawrepribix,LUTSI sHpyaeHTHoro Hilpy-
ca, BKJ11014a51 IlaTOITHHOCTb JIJ15T 06e3131111 (MN_, rct40_, S_, d_).
IIITamm MeFi H3MeHHJI S-npH3Hax (S_), yrepHa CH0C06HOCTb pa3-
MHONCaTbeil npu 40? (rcto+), cymecTrieHno CHH314J1 naToreHHocTb
,LI,J1S1 mbaueil (rnN? ), 0,5,HaK0 HeKrioHnposaHHaH nonyarm,HH, npo-
tueutuaH 10 nacca>xeR npH 25?, coxpaHuna naToreHHocTb Jii 06e3b-
%H. ,II,a.abHeciume naccaxcH urramma MEFI npH 23? npriseaH
npaxTrniecim nonHoil noTepe naToreimocTH Bripyca LJ1I mbnueri,
no.rwoil yTpaTe CII0C06H0CTH BHpyca o6pa3oBbmaTb 6.rmunKH Ha
xyabType TKaHH no afapOM KK npn TemnepaType 23?, TK H npa
360.CApyroft cTopoHbi, urramm ,II,EM Aax{e nocrie 20 nacca)Keti npu
25? coxpamm Hell3MeHHbIMH Hee HCX0HbIe reHeTwiecxne IIpH3HaKH.
B KOHTp0J1bHbIX OIMITaX 30 naccax(eiA LuTammos Biapyca 1.10j1H-
0mHeawra npH 36? He 143MeH11JIH HH 0A,H0F0 H3 H3r1eHHbIX CBOACTB
impyca.
3. Kam-mammal axam43 nony.rmuHri HHpyca noaHomHentrra, npo-
Berternmai Ha OTa,ejlbHbIX nacca?Kax y ILITaMMOB, H3MeHHBILIHX Ha-
c.rteAcTseHHbie cHoficTHa, noxa3aa, HID H3meHernisi CBORCTB nponcxo-
afiT cryneHtiaTo. B nepHmx naccaAax o6HappmisaloTcH gacTHubi
sHpyca, H3meHHHunie KaKOfi-111360 OLHH reHeTruiecrunk apH3HaK; c
73
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26:
IA-RDP80-00247A004300210001-9
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26
IA-RDP80-00247A004300210001-9
iiduaiKell B TIOHYJIHLUTH BbIHBJIYHOTCH HaCTIMbI,
H3meHHBame ,llBa H 6o.nee reHeTHT-iecxHx npH3Haxa, ripHllem KOJIHtle-
CTBO TaKHX T-lacmu TIOCTOHHHO Bo3pacTaer. Floc.me AOCTaTOHHOTO
llHcza nacca)Keik y no,llaBnynoillero 60,THALIHHCTBa HaCTHH BHpyca
Ha6mojaeTcH H3meHeHHe mHorfix reHerH14ecnix HPH3HaK0B. OAHa-
xo ,lla)Ke nocae 60mlluoro llHcaa nacca)Kell B BHpycHofi nonya51-
1414/1, inmeHinumeR Hac.rie,acTBeHHhie csoficTria, o6Hapyx{HBa1oTcH
eJAHHHI1Hbre coxpannBame He113MeHHbIMH Hexoropme HJIH
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FlpoBei.keHHme HCCJIe,q0BaHHH noxa3a,llH, HTO H3MeHeHH5I HaCJIe,.1-
CTBeHHbIX CB0fICTB B npouecce naccaNcefi B tcy.nwrype TKaHH npH no-
flIDKeHHOfi TemnepaType Ha6JII0JaJIHCID TOJIbK0 npH HCHOJIb30BaHHH
maccHBHoll Hrllimuipyroilleil llo3m; naccaNca KOHetifibIMI4 pa3Be,lle-
H1451M11 BHpyca He H3MeH51JIII HaCJIeACTBeHHbIX CBORCTB 11011yJI1HHH.
4. floayllemmie ,llarnime 1103BOJI5IOT CT-IHTaTb, HTO B OCHOBe me-
XaH143Ma HaCJIeACTBeHHOCI H3MeHHHBOCTH BHpyca TIOJIHOMHeJIHTa B
npouecce naccamceii B xyaurype TKaHH npHnoHmKeHHoilTemnepaTy-
pe ae)Kar CTynennaTbie myrauHH, HllllywipoBaHHHe H3MeHeHHbIMII yC-
TIOBFIHMH BHeutheil cpexpi, c nocaellyfoutell ceoentHeti HaCTHIA BH-
pyca, CBOcICTBa KOTOpIDIX OKa3aJIHCb HaH6oaee a,llexBaTHm H3me-
HeHHIAM yCJI0BH5IM. M3MeHeHHH HacaeAcTBeHHmx CBMICTB BHpyca
110JIHOMHeJIHTa B npollecce naccaxceil B xyawrype TKaHH npH nonH-
}KeHHOn TemnepaType 3aBHC5IT He TOJIbK0 OT YCJI013HR nacca?Ka, Ho
Taxxce OT CBOIACTB (CTa6HabHOCTH HaCJIeACTBeHHbIX IIpH3HaKOB)
UJTMM BHpyca.
H3riEHHE BHOJIOTIPIECKHX CBORCTB BHPYCA TPHIMA
ripH BIABBJIEHHOH H JIATEHTHOR HHOEKUHH
H. 17. Kopflotuetfico E. B. Czawmagn_
(Ka(beApa impyccoorim Kliesocoro rocyAapeTBelmoro yHHBepcHTeTa)
B paoure H3ygeHbi pa3BHTHe H- 6H0JIOTHHeCKHe cBoAcTBa BHpyca
rpHnna B yCJI0B145IX KYJIWTHBHp0BaH1351 ero B opraHH3me }KHBOTHbIX
C pa3TIHHHOrI HyBCTBHTeJIbHOCTI310 K TpHIII103HOil HIP:PeKHHH rips
BbIHBJIeHHOM H JIaTeHTHOM TelleHHH ee.
B onuTax HCHO.T1b30BaHL.I 6e.abIe acivin{aHcKHe X0pbK11,
KK opranH3mbi, HyBCTBHTe.TIbHbIe K rpHnny H Aalontne xapaKTep-
Iible TIpOHBJIeHHH HHCPeKHHH, H }KHBOTHE.IX C ecTecrBenHoil HH3KOff
BOCIVIIHMITHBOCTb10, y KOTOPbIX 3apa)menHe He conpoBoKllaeTcsi 51B-
HUM 3a6oaeBarniem, HO Bnpyc mo)KeT HaXO,LIFITbCH B JIaTeHTHOM
COCTOHHHH. AH.a.noratuule Ha6mo,lleHHA npose,Ttenbi Ha BaxuHoHHpo-
BaHMIX mbunax c pa3J11,111HOR HalIPMKeHHOCTIDIO cneuminmecKoro
HmmyHHTeTa.
74
Declassified in Part - Sanitized Copy Approved for Release 2014/02/26
riA_RnPRO-00247A004300210001-9
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