THE DETAIL OF THE PATIENT AND INVENTION #178336 WITH ATTACHED ROUTING SHEET

• Document Type: SPECIALCOLLECTION
• Collection: General CIA Records
• Document Number (FOIA) /ESDN (CREST): 00146124
• Release Decision: RIPPUB
• Original Classification: U
• Document Page Count: 6
• Document Creation Date: December 17, 2024
• Document Release Date: January 15, 1983
• Publication Date: May 2, 1955

'
. � . . . � li�ii:ST .i�liCIED � �
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ROUT I NG AND CONTROL RECORD
LDO NOT DF.TACIL Fnom ' ON /..C),IN 1 i:OCIR.IENTS1
f
. 2 i,!..ay 1955
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Attacr.fd to.03-A-4C216
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CVS /..eNS
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1 Vi...A.�,tr.:trt.1.1 v...ii .::( Pr:::;*4.-:4; ro ,��� �
CC:-:TACY D1VII'3IC:-:/C'D
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Jztpanc:;0 ,;().:crn;.ii2nt
Tho let,;(3.1 or tho P;:.tent and Invont..lon 0.70336
/
Invont.or: :!;C:,n;;U Abo
it TO:,o
owsp;r: .
21(.7 "Lt;11:;hi�Ku
"The :.1..inuactur5n:; of ni.ni cf.:cuive content ot; erot by
artiricial cultnre."
I) The abract of: the nntnre r.na objetlt or ,:.ho
Tno pur:;oc of thIn ILv:..:;tien In to p:.%re ver7 eif.:ctivo
new :later-MI whch pozonLs c:c.tr;z:ely pod...ul ao!..7.on to con�
tract.thd 1;tt!rnn cnit%ro lf or,;ct b:tut::ria
rinIch pr:!�;.toes xrd ::en;_tral �
proec44res NN C,I1OW:31 iirLt to csl.t.re t!ie er.et bt.tetkai
which nroduoes .loitt, on the
jflt)J*::6j1� bd:!co; socentAly tho
tion o_ $R. fro:: colony or eultor::1
(in the t.xr:,o;,ion in dal';..d out :re:1 li-,1:c1f or
o;:tr:.tot frn-.1 the !Clu:lin:t which p:...q.vicu:%1Y.::th:cn'Ind
lotd) nelvon:,n� in al%ni'o:u noluno LI,ch Ln
chlorofor:1, benz.:nu, antnell tolr.t.,fle�
c:.�rbon dIo'llfi�Jo or cerbn totrachleri.lo, otc6 3r0 carricd ou,4
1) The c.!':,rne't in 0.;:cold by thn
nA nod eon1L.1 to :zivc
chloric zcilA� rulfur-!.c or 1,:.1rtnre ac3.f.t !!to. To the acd
nolctlunp oLc.' ziatea-Lal) was afldetl to Iii...!c::�
oitato Az;rocl.,vin iron iLipurf:ty.
�
II) DotAfAcA c::o1:1%;.1tf,.en or t!lc inv:!nti(:nt.
Wiis invention tho ;Ithtcw..1un to the oviin�.1 invention in vl!lch
tho bcteria wo cultltrvet in the j'aLcc cv.:l ether crti-
1.icir'7.1',:ur;:1 bay.en ni..d the ev:I.t1 ;:au direettr:Ict:A
w1Lh or:;z1:210 nolvent, ovath ether, qh1Oroi.er:1, bef...enjp ctcc
which nyeaot ;1IncIb1e with watr or c.:4':orbed by ads:;:i.bent
alIJ:J.Ina to zut active oc.;;:one3t o,f r.;ot, but after tho fur�
ther rcsev.rch, tho h.Acto.r, producou hcw onaloid--
ALroelavin, cult.:;ruiA id :ialt juice, KUji juice or other
�"4,0 ��
1):(), Lhon :vcri th:.o thoro
of cfmtv:..:!,ton of utorro aad tnm do
rot ond to ..11t; ):1:0da no th.l.ti t:nv
123-2o)�
(t1:11twy..1) :Ina Cho Lr,x'...11::a :zI)noion
:11L1 tho A�.
iho ro:oryollid w:lv colorIGnn
citric
cicid or roont. nol. .%11 :.rorn:i.4.1 or or;;:-.:.ie
nnd their :;01n4ion did n)t Z.,ost. it bln.o o0101.a--.
tion to nulftly-'o 1
n�te. It. iv vory df..fficly vol. in
nUd. vol.. et%yi LIcot..*.tey Cf12. Thu
telv.c.Yr.9 ntt. shoa tan:.,,linityo 1:134020,023
pharooloij.cal :%ctioa 1.kon Lo 3.nnlat-A!d
Ltorun of r.-lhbit or i.:;114Crl
tf..on or A,6,0.avfLn fro.; tho b3etorlv.1
bneo a;tich c,11t1;11.:d On oull..11 bz1:; nn nn.it
c�,:.!;vr *0:1;_:�;:3 c: ''L'.'1 out an
�
1) 1:1f.ltr5..-:1 fro:; ou1tmr.11 c.1t.o 1.):: the
71.1. ?.,.).; t",-';1.-. an!
fn':/.; by ;;;s1.,.
w'.th Aee':ono., I r.:..dinr22
CC,2$ ;;;.'1;$. to S, .j ;.1lon :Ize:.;vry
Co'colo:;�i:;ey co:t luwly to ne c...oy
tc11 or crynt:111f.no of .".rool:cr.:.no And tho volu:Aon of
f.n cr,:anio .133 nh:::cen
n ;'ti :11:rt:1 ;IN ;�')(�r.+1. otc. rd.0 1:11t.n
iteLd
thun n;:nf.n c:!,;,?;.1c.td thn 01.�:::to
11%.1.7.:1,-. to cri.:0
1.7.o1%-on dtIrini; ..hnn !dho repo;Ltd
1.1f; connvodp .;.owd-.:r of
AL;roolavin vuonra'.;cd out*
nun t,ho If.710 the ;:cid.,oet frel tho
nolvQnt oo:In'n�;
tho or.oe no of
the cond..:.:�u:Iton of tho coll!tion nnd 1.c:v.a.voctini: to tho cv4i.ount of
the cLduncd.
� :
ppt. filtv..rud and wavhc:d nddlni.iolvod in the or,;nric nol-
von!;, wM.r.01 wan.dry when n.,:orry, ;Ind ori_;.1n5.0 oolnt:.on wan con- �
dcIncod to 1:1-to tho pf.r.rdi:r of AL;roolvvin.
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1:111. -1).:no rron tho bnotorTh-coIon;.r:,
�
or 4It'iokit tho wao 0::1/rnotod win em3
wh;.en Ar.! not :C.:;(11)13 w..t.Lor or ndnorbnd on t'.:o 1U j::: etc.
1.t wnu oranie )1� :::t
�:1,1 ;1.th ehnconi thorcl;1:7�
t%-n tu or...,.anl or 6110 :)0(10)! of A!,,roolavin.
nr It :*:to shz:1:en with no acid nnd the nold nolution vmsa:C nndo
wlth n'1:loniv, or utner a1::.11110 natcrIal to pat.
rre2 the 1-11)... no ppt. vno fliturod nrd w-.1:1:0(1 �
and cdio,:o1,..'ed in no orle volvont olzch ae otht!r, benn0D
aectono or nethnnol c.n1 the :41..l'Aon Imo dried -when n!!eon-:..-y and
con:Ivn::od to civo tho or;rot,a1 or tho pow:lor
ppt., oryct..,.111n..) or no or:f3t.n1 o1 3CitV.11 o5tained
fro:.. the 1.mcZ.:riy. or c1:1tnr-hn3o co bLIC6re .an3
din:;o1-....:d in 1-,he n1'.;:nnIc nol-font vlich au noth;inol
or ror the v:1,1riztal1ntl:.on and tho 091UtiC,a Wr.:1
co;:141uod if
ol'yotal obtnid wator 'Jan nO.:.lod to ito oolut.;on
prouoduro wno ao follown:
1. pro.Anos Ai;roclt;vn, vac pin:oed in tho
v2n)t juioc (n?c,3 1.):1 5.h) and it -.nu nt 200 for 37
Then tho elitur!;:l fro:i clo oa-
z;01:C.,lon a.1.1 11r-ooe:1 ,) ;,..1:t; to
oc,Inny puivorIxod tr=tod with pot0 et:s..Gr to rt.:loco
Jot w'.th .er to
1 vit'l other tn con!..P.:1_11.in:: :211:n1oLd In
wau.df.:hydrd ./t4 �
1JI:ovt.I)flwno pine,)d in tha
tor *...0 :-,c7t:rnto tho pw2;t:r oC colutf%on.
pov-At.r df.ounlvcd othor 1%na
clOydr.-Y;ctt nnd thc,n t: id to
give t%e crynt11-.,.no A..;.;2o...11nv:1.11 (0.4 g. of cryotal ov,ory 100
c.o c1,tdpowdc.00 /
2. 5.0:; or ouer000, of potrIvoil:n hycli'oon ahor:pfIntea 000M.
%
nulfne, of al:lo:111:::2 ,cuceinato in w,aur vzo r:n4o
pM 5.2 'oy Mfl of uttoonio
"0 W.ilti*V.0.....1;ksol
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cul q.loopu.ol uuTI!.:tta ot:A to solso couc.,;:uog ao
CV;:0 cv oti; pGoraq:,:o
� oorq oul anIno ao
';oDync uw.o;uo. uo;uo.m41.
� ti47puu 6Y6�691 �41:d vzn unPlv)IT's= 0111
cro:e.; 01. 1141.:t0ll
*(c,cuq-oallvcuo cln zro 03 cocy aGe I.,.!:;u1.0,3o en
pul.uI CLt
uwAu--0.;n otri cicanclac.u77.2c,qU zouGE:A
Jo uo-fll v!,7i1-0:1:1) pooco ucuiq
1�1: ,yonz!'
vaul. puv.
cs!t
ci utrioo n;uot:Lr popp
ol 17,.;tiv 3TIC;00
Itn,autiqo a or,L. ,,:vprAn
WC,V, pUr: ./.111C1:00 ,:lsouyu
g�to0p7! �pl:Gyyn :;0 22u o*c: .7.od
30 en y) p:1/4t!caq Gu:c ub-1:4n.cor.: pt11.: 1.71010o
ou, poao'4ITj oul utlq. uo
*4%.-0.!:oppu oXnpT JOJ qn uoto
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pJr: LI ao:: (1 gv:it puu E;on-lvoad
cv;az,g ri�t:n Tv:if)3,11Y;a7J
oppi:t1 Ed o;: rulcav:doc
Jo .)01 puv *1%::; ;:o couzozid Lnian7;lod
ju fto'.1.i:lzurn 3o 0. t0) uov:;.11-1o1 ono:)abo 0;11 *C
jo slop
-;:od GAT 04 pz):.:uopuot) pun po:;w0Toop pot.ivar.::%csp
cn:1 t.toyoa Gq.4 puu poqoyal. IrioTu:x.;
.711T1Cd 0:30 4.-.0117;71- a:11!,1.1 oo
*2 col 4,10.m) t:oaj *2 (00) u.;AvIor..,a7v r.o..zuop
uoluToq pri,412arrlp paow.,o Ui
:141 pun uc;,:ln.yon t:3uo=n, *1;p u.'� paurnirt p;lac:1-(3
01W. *1-1T.7.'1elui 0Y4 V:0,73 r.';.3.":J uTL7Vx-z:-1V
Jo ti. 0::7!!.i.toap
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u(1..� oz!.1
uogl pfro pjcp
C' 0C r)ti uv..fq.co :twc1i. p:,%:noak.
Uq rAvi t:oJ. r.=:�0-ion
(' t1;) 4;:"1�101
- ..10:%t0 ov:to
'o
����1�: e�f.! :111:01;. ;u.110
tho
imo-pulterod fesi Coo
j,11co GI' no !)!Af'.o. 'The 0.f.lve o0:1-
:1c)::o:It of ov !..ho culturo'bo,
wi.th tho ov!..;:tNIc
no bt:::1111-o onor,
r,CCt,.:�:10:, cm') c.'e Th.()
1). jc.:01er.!..::i.:d .a..;.th-sb'.711c-eoz..1 or eo. nvA
eond..w:QJ n.r 2) the =.:o.:p)nnin
c:a 7.2:7f:!11, . To tho oolut.lon
vnq or other (1.1o.1.)_ne aubsta:-.eo to fyce�
tho .1:ron ii411:rIty to oletn,.n o;.j.it,a1 -
nue ne.a pd. wnLoh.-hno vury powerCul and. eon -
Lr:Ict u.torun.
The
Lt.; :..taod whieh p-ro-Juees ntra
.)!1
er .1ture eol,Jny or 1..he
(:1.n t,1.10 1:./ur er.:;!7'; i,.onw;.,o deno
the 1i.11:;u:c) :....elutton or tl.lo ew.ipon:Alt wns :,...1.corbea on ',Ito
;1:11.
Tho ..:ao 1) doeolori:;c.d
ch;lroo:11 or 2) int:o tho :Acid auoh
aoCk.,o.r o!..,c. To thf.1 zsollItLon
adOod nq. :7;o11,..:a or ei-.t;.,.v to f-).:Jc
zlna ppt.
pA!.:,ont .:;h0 1-. Y1,1-1
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