}am-cm exit-procleet� - - Date of Cr:Act-Al _ PericA, T Antiga-of At Author intim CovevA To - Ilur,Soer . eta. igati 4 it� z et g 7 - 1 tim�awa.,��+.�� AittOi Autbarizttt lona _ Cos,c,.-472-1 5.7o Peri _ TeNi _ _ / I� t I ...: �...........��......4... .............._.................... ��.�� T I i 1 I i � �1-----.o.......... ... i rh A .3.1ont Crraig.ltion 17.zouiltof /1,��.-�� ....-4 fR, ,,,, V-� ..."1..",..... - - _ - r, ,.-...-,s---- Inir,..-.....m.-.� rai�� ...� we , ,...r..='.� ..., ....._:, . ''' '''' �,L,,t,....,......��..�,�.*.-.�a�� ....,�� ........� ��-��- ��-.�-� �-��,-,.---��1-.....' a�� ....`��...r...." ...�� - 1 � alie--14.... IA a eziA t _ _, 10 4.;11_9/_oi411_97.f..l 3/5/ _ _ _ ..._ ,c... _ or, --1-- -/I.e. ... lit . t 1------- ; --, - I so- -;� - 1- - _ - r , ......0e0.7..va.,41....- --4t�)---------,-F'--- /11 41/1*.1.00. _ I t t I I / i �-�-.........,-+ /..a...o......... _ i 1 * I / / S ............ .411,.,... ..... .y. ow............./............./..... /..................* . 1 ..... y i "trl'.2"anitS � ac: � I - _ Am./. (When Filled fn) ACCOUNTING BY INDIVIDUAL SUBMITTED gy , PEMLZRA ablprAleTt i 2.43 DATE � VOUCHER NO, 2.12 FOR ADVANCE PERI� OF ACCOUNTING FROM IL julanzazt: .WOrg: Follow Inatfoctiona on 2.03 TO 3.965 I. CASH ON HAND BEGINNING Of PERIOD _22._Coetobekr 2_39,83600001 S, EXPENSES THIS PERI00: 2. OUT5TANO9NG ADVANCES 'BEGINNING Of PERIOD vOuCHER NUMDER DATE DESCRIPTION A OUNT 2. RECEIPTS THIS PERIOD, L_EVenBee___�.�_$=.-..efthgt3AL______S I UNDER DATE OE CRIP ON callalextIot 390836.00 TOTAL EXPENSES n 16.00 . REFUNDED MER T I ICA1311 _ j 11 1 CHIECR /NOWAY GAGA 1 1 11 7. OUTSTAND HGl AovApipcs EN loll PERIOD A f chl fito011il 1 I I I A. cA5 ON NAND IENOHOF PER 00 ON BALANCE DUE APVAN E III I A. I TOTAL TO ACCOUNT FOR S j8 36.1pou, 1 7 1 7 TOT4L ACCOUNTED 1 111 11 I I 1 I �,,� et,z $ 710.P,avoNnor ,o,�4,,�, CERTIFY F SAEAVAILA LE 11 1 APPROVED 1 I III III I /I eer4 ly thai ..1. Al!f0A74flurrovl hinted hereon an. 09L . oo f. NO. I CHARGE FAN ACCOUNT MO. I I 1 , 11 I 1 11 1 1 II $19*813640 DATE I � I SIGNATURE 003YME:f OFIAP 1 001150203 1 1 *OVINE 1 r 1 A OFF IC 1 C 1 Tri) on 0 Fre;dliF Inn'I S00 1 'IM Alqcorff It' of IfIhrr Farman 14 6 for gfi4gell o Ihs LiAfiruA werealinmort44 ill not ltostmra, Seen a efeortoFt: for rocelved, offAciallpu Ithat 'payment and that 1 1 1 - or DAYL SIGNATURE Or AUTHORIZING OFF CER CERTIFIED FOR PAYMENT OR CREDIT s GNATUR or PAv�1111 I I , ATE I I smowATuRc or CERTIFYING1 OFFICER I 1 1 1 I - . I TBD/BB 1 1 SPACE BE.LOWHIFOR EXCLUSIVE USE OF OFFICE OF FINANCE DESCRIPTION.ALL. OTHER ACCOUNTS 13.33 34.39 1 .1 162.47 1 49. SO $1.34 H 55.66 67.70 71.60 I I I� 26.3 v/A PO. STAIION CODE 41 f I ; 001. 1G 1 REF. NO 1$ PAY CA ON 4.F, GENERAL COST ACCOUNT . FAN SYM90 I OBJECT ICLASS AMOUNT I AOVANCC oescRorTiom - 'ACCOUNTS 03.27 1111 $.�,.�,.,.. ...... ***** . :1:.�:' Rce.4Py. ........, "WC' 0AvANEt Eig g E ;..........PEP.I AG6T. IN . ........ . e".. N� ..- ...0. Com S , YR. LEOGER ACCT NO. u. '"' .o. .1. 606 CA ; .6-1011-J 7 MO. .-�110.1. 60.70 DUE DATE DEDIT CREDIT .� ; . :/: :/.4..-St i. :I ././ ?� : ��I . : . ...2..�) 7.-- / f. / .1 . . . � � � � . ********* . * 1 � _ . x;/: :--,to,:jo: :/: :.�2 : � � : : : 7.-,,, - 17/, . . ...5".; 44116 * � � l� . . . . � � � � .1 � � � � I 1 � 1 � : lo, .� � . � � � 1 . 1 . . . � � � . reel. �� h� � �� 1 � ATE PREPARED 6 - DATE REV (IVE0 AV I TOTAL ;�,,,I. . ...? FORM 282 :=1:: 4.65 vioui 1, OROUP I "trairorirtrelYOZIMIZIP- I I - ) , � This Jilt* certify-that I-have received an accounting fruit HAMM &lb-project # 03 far the period 1 January 1963 . 4,06tOber 1905. Ite - accounting refleatexpeasei ia the amount of439,843.29 agaiast the paining grant balance ar139,834.00. The residia1ha3.sme_c1412.71 should be vtittea:Off as Wag inprecticle for collection or detailed accounting.: The acCounting statement end paymeat certification vill he retained by TO and will be made available for review in TaiLnetessary. I certify that satisfactory servicaa revesenteChy_the accouttIng have been received and that-to the best of myknodledge and belief the funds expended:were for the purposes authoriU.d by ,;he project approval A. � , . � ,t t� � ..;'. 4 � K . - . � 111110221.0, Consumable Supplies_ PICA Telephone &ITtlegram Tubes 6 pipettes Misc. Supplies E lee tr ieelfSuppi ires ChelliC31311' freight bills 0 9;089,17 650.00 7,710.00 400.00 1,995.00 250,00 1,458.00 - 1110-40 2160.00 200.00 _Jur 16,157.17 8.34- 5.55 575;43- -4 066.35' 115.93 176.07 15.33- 'Filter apparatus 111;16 Culture 3 5.99.71 Flasks, stoppers, syringes, etc. 357.99 Repair on Vacuum pump 8965 ___ Test 59.50 cube mixer Service cnia-Sartorids Balance 120m Totel:Censumehle Supplies 3003.11 Travel 1/634/6/63 /3/64-4/4/64, 0/19/64-10/24/64,- Continued inet page. 457;04 27.9011 21.82 174.25 i61 t - Cost_ Account_ Object Class_ Retrairits and_References Obligations Obligations tInliquidated Incurred Liquidated Balance /fij&1 LTA 070 era ex e cvh : �� NAME OF A VA C E , DATE ' Am0 7.1.*L00.00 FEEHER No, (Finance one only) REQUEST FOR ADVANCE ,30 jnnityAxy 10G4 $20..000,00 ..*. � OF FUNDS � PAYARLE TO ' taChad.-412.- �� , � ' ROOM ' , OUILDING TELEPHONE EAT, , PURPOSE � 4 Advance t - or funding )ULTRA SubprejeciCiA.13, C-4--....."-=----� invoice 0 2 bleb activity was approved by C on 25 January Pineal Annex attachment B. ....- .ai �_.'7..SD' 1964. � Accounting � . � . to be in iFeer anee with ITKULTRA I 11! X0 1a_tes_ttlian 1.0 February 1964. , , 1 11 [S7AirOS �I 1, 1, , OF OLITSTA I li 'II 1 III 1 IND ADVANCES ' II I I 1 ,i, atrAelf At I ko ehl �leportingipoint Noy lunexpended alp fully; s balance. account fl atetf sathorixe for ti and Ibr thedu deduct advance !dale on by submieelon Of woucharc;eactire frd cReekodNolow. Init,Ie event of apifell Fr up eale , 'to effect set dement,. of any onexpended re to 7. account ,iiimil balence rotund ...." I ATE I 1 II ' I AMOUR uwAclaxon/pRwAce, i , REPORTING POINT 11 I 11 I 1111111 '1 Ul 11=I FINANCE DIVISION - H DOUARTERS ! 1 tolq 1 �N I 11 1 1 , A 0 R VAL ASOUT� AT DESTINATION 1 HI I 1 I I 1 II il I' MONTH!. EMMA . ON Or EACH THE LAST� MONTH , 1 1 I I 1 � REQUESTING OFFICER I I APPROVED H I I I 11 1 RECEIPT FOR. 'FUNDS ADVANCED': DATE SIGNATURE OF APP ovING OFFICER', under so Cc whom nouledge receipt to he cowed above. of funds for the purpose in the autatot]i atited'andaccountled elatedlhere. for ATE 1 I AMOUR ' F CERT 1 I , I * ORLIGATION REFRcNcE NO. � . 9� 0 CHARGE ALLOTMENT NO. 4125.4390-111902 CERTIFIED FOR PAY ENT1OR CREO T I ATE 1 AUTHORIZED CERTIFYING 1 OFFICER $ GRAVURE OF AOVANCEE I I I I 1 ATE SIGNATURE .. SPACE BELO FOR EXCLUSIVE USE OF FINANCE DIVISION I � 'ARCO 111Y ... .... � IREVIEWED DY ....... _ .... . _ VOUCHER O. � 2 DESCRIPTION-ALL OTHER ACCOUNTS 1 3� 3 43.4� IPA ODE 47-57 GRI. �cc,. I Isl. NO. fl GENERAL R-47 1 ALL.1 OT5.11 OR r Cosi , 1 ACCT. NO d 1 .70 ' OUE1 47e II. SO AMOUNT � . � - . . ADVMAICEIACCOIUNTS 1 DESCRIPTION - 1 19-27' , 11 I.LIT2NUI. 1, A . o . .',... No. STAT !ION 7:7 , Pokey ..00l. I II, ,�' 1 EArei�cm42 CODE1711 7-1! C � 'It'aPii;i4 .o .;I ,,,,, ''' ' . ftfl"I I 1 IlirEOGE0 0 In I 777 . I - 111c! - "' '' Id on 1 Ct k�*, tiElei A $0 !MOOT Colt00/ �Op..., re' NOOSSeT No. 11 Ilicy 0,41I,1 I - �I I .1. 1- ' v'trEnirti:7. it ..l. tt I, t , 1 ,1 , � II '1, I .", 1 ' ' #40 ' 1 1 ' .. '.../.� 1�'�� I''1^ ,/,.f...lf?' , , L." la,. .."II 7;1; " I, ....... 1 1 11 1 I 1 I ' - I 1.1:"..:"+ ,..1 ,1-1....4. i I I I I li I , I 1 1 I 1 1 1 1 s 1.I ,- 0 vi - ' lil 11 ih J . . h r . , I ,1 I P � �..- . tr I 1 .. . `... ,I , II . ,t. II I 01011 � � TOTALS ,, 0 I 1 t 1 :1 Iii j TOWS no. -.45.190 AXSCUST PREVIOUS MOTIONS. .�� 60YIER0000T Tnerfinis �Tract Oyez 0-040h) 1 UnVORANDUU FOR - , Under the authority Id in-the m-�mcrandu dit d ',.pril-1953,-- -; from the DC! to: the DOA and the, extension-of -this autboyi.ty in fsub- 4 scqueat memoranda; SubprojeCt_143 has ''ssn approved, $20, 890 JKL:... -of the over.--all_Project.UKUIZTRVfunds ha" 70'n cOligated--1-0-cc=v trio subprojecv'- r.mengt1..s and stiould-u-'-tle__r,Iirgoci-LCV CO 4125.1390=739.0.2 - Concur: '0 pstE Concur: APPROVED FOR .OBL 'Da Tiff OF FUSDS: 13 N ifig4 Date: Distribution: Orig & 1 - BB es GROUP 1 � _.Exc1u-ded7frm 7 auto7 Obligationi- Obligations Unliquidated Incurred Liquidated Balance eDCLt�t and _ ensin of this-antho,rity ,tu-brre,i 3k,ct 143 I.?. 5� een. approveL, = 01 .144 _ rnernor,ind - IKULTR4. &rids have been obligated to cover the' 3125.1390.T.3902 . THAT-::;;NZ;,S�Ars: ' GROUP I Excluded from matic_downgrading and �aeifioation � , - , � NAME OF A AN DATE * .00 VOUCHER ,NO. flnence use on FFOUNRDASDVANCE 7 REQUEST lie , OF t AYABLE Atejlitititieblegt iftyleetnt Inst. ROOM RCM-DING191;$ ns TELEPHONE CRT. L PURPOSE � .7, Mesta. to NIA.VIA gibproject 1b3 Undo" IF X far siottrIt/ approve& by CAM) am 17 2*.iceveter 1962. ,�:t , Acootatiog for tags arroreoca it'll be in acoordusoo with atorobsetat A of NICIZMA nava Aare*. ... , .., � --. /Imam frevara tba cheek to ete.terottlefritoloarival. trench tbzwilk 11:30bet7et Crater Irs 3 aleatory 30631 -,, . --. STATUS OF 1 OUTSTAN!ING ADVANCES 111 II i 11 . I agree that I to "ff r�Pol'thill ORF ROFFPORdea will Point Wane. fully minted, 7 4402,10,1744 account for this ad by auhuieigian and by th� due (trete checked below. 41160/44444 Ili.* Hy priari of unuehers and refund 0/ onymonorponded In the *rant alley faIiure to as account end to effort sot fleapit. 11 Ir I I 1 1 11 1 beloonce refund � 1 h t AMOUNT 1 1 I ---, IE S CE II I 4 II I I REpORIT1N IPINT I III I I II 4I1 I I I I 1 I I II I II DUE IIOATEll I I 1 I I I I I I FINANCE OIYISIO 0 NEAOGUAR E 1 1 II I I I I 1 I I 1 II I II II I I I .' :7.1 �OR I I I I I 1 I I I ON ARRIVAL ABOUT AT DESTINATION I I I] il II 1 I I I I 1 I I I 1�I I I I I I I NuNINLT WORKPAY HI 0F1 oN,TNt EACH LASTIII MONTH I I 11 I I I Il 14�1 I I I II I I I I I I I I I II 1 NEOLIEST ING OFFICER APPROVED 1 1 1 CEiRT FOR FUNDS ADVANCED 1 DATE '� � 1 15!IGNATuRE P 1 I 1 OATS SIGNATURE OF 1 APPROVING I �- � OFFICER 1 I 1 7 44knowita.244 0�444.1,44 el fund. dr, the oo under tor bia wed for ths prowlers iota ea *shown *bore. 001100101t *cloud 'stated accounted bore- fo r 0 '� CERTIFY FUNDS ARE AVAILABLE . .. 52:1241M23:23LACItSki DATE AMOUNTd 0212,14AT ION REFERENCC NO. ...............-, CHARGE ALLOTMENT NO... CERTIFIED FOR PAYMENT OR CREDIT I I i DATE 4, , AUTHORIZED CERTIFYING OFFICE SIGNATURE of ARTA/4st( ATE. SIGNATURE 1 . SPACE 0 ELOW FOR EXCLUSIVE USE OF FINANCE � ION RED BY �Ii . REVIEWED BY 1 ,...,:- VOUCHER NO. T� 12 -, 0 coital. or4- ALL OTHER AC 01P4 S 13.3S 4. 43 45.44 47.S2 1 3 5 q 31* 7I� SO 21�!*).... tel. S3 � STATION 0,4. ir 0 zr. WA SO.. - SA. 0 -MONT *74**A , 0 DESCRIPTION. �AOGANCE ACCOUNTS Ss* 27 . . . .0. � � �� Mo. . PROP.1114: � �r� �� [�-��-��s-- . .r:17 OI I III LSO. ..:!.....Lo., 4::::1.I.t. "V I c �. �EDOE - AC7'"1 �4� ...C. . . 04444 0411110 I CBCBIT .. Ifl II I I Il II I I I t� 14.;.s. PAR eev no. 1 orlv coot ,...1-,1 , , 1 .1 � I 7 r' II .......* jt I G A Fi�0141,...e" "5- 4444.4.4. II I , 4 1 1 I 11II 1 4 I 1 t'l� 1 a i V I I iil Of 0 II' 11111 III I. I I II I 1 C,Ii�I .11 1 7i Et; 4,74F .......... 4 , � II 1 1111 I 1...`t I 1 1 I 1 1 'III 011111111 III 1 I I 1 �I 4 . . 1 I T I I II I 1 1 I 1,1 1 1 1 I 1 I 1 i I i 1 1.1 I 1 t I t i i I i $ I t , I .,. � ..� 0 �..---,.------ , ;Aim ill Ill . o t" I I I 1 o t II 11111 o t 1/!' i1 1 l 11 It TeTALS i 1 I 1 1 FORM .2.01 use opevoevo toev��.., . 1 AMC OF P A DATE , *62 SIVA36600 VOUCHER NO. (F/R4Fce "IR FOR ADVANCE 19 Det ...a. ...x. ,79 ,REQUEST '�,..: OF FUNDS * $ g. PAVAOL - ROOM SU1S.ONG T LEP 0 E EXT. 0..........., PUNFOSIL -r *, . , f- . . AdraVF040 to 14103Intik titlibpro4iot 143 Uteri*. # 3. far mot%v ty approve& by' MO frd. Accountimg for tbin edience 10133. be In accordance ifltlx ftttecbment A co? ICULTRik ,;' � ,.. . -;�kg., Please rotifer% the check to CbierirSDfritological Broth tbromA 1311D/S4get �facer , on 3.7 December 1962. /local Annex. , by 3 January 2963/ ,- i ' STATUS O OUTSTAND 'I 1 011ii III IN VANCSES 1 I 1 1 1 / a�,,.. //H.* toirmo reiirrvino any'unoxpoododi s fully pranunt far this polfte prated and by the due bottoclo, / authorise deductilon advance dolts bV oobolooilOn check.d below. frosrevg rotary of ln to effact voucher. and re fund the averse *fop failure kletelewent. 11 of ill 1 any unexpended balance to so sweetest and refund - 1 II hill liv DATE 1 I IAMOUNT I I top I I I 1 REPORTING 1 p INTI 11 11 � 1 II IH 1 11 V � HI DUE 1 T I III I II �,, 1 I 1 ,... 1 1 H 1 1 *Net DIV I ION 11,1 ,ii, . � II ADODART 5 1 ERS V 11 I 1 1 , I l , 1 1 0 i Iff ' II I I � I I I 11 I I I � I �1 � 11:14 ON IARRIIVAL OR Asourf IAT 1 DEST1NAT I II 0 I I II I 1 1S rl V V 1 1 I 0mT*Lv ORXDAY r or DA THE LAST 1 lEACW MONTH I 11 1p 1 , I ' 5 ' 1 I I I 1 I V 1 I I 1 � H A, � EQUEST I OFF ICE I 1 APPROVED' I I RECEIPT FOR FUND 1 ADVANCED � Its, - ' 1 SIGNATURE , � i DATA � SIGNATURE OF APPROVING OFFICER , q' r acknowledge receipt of fonds ln otho assort seabed her.. under to be used for the porpoise istatetlaod accounted for as shown above. 1 DATE . A I UNT � .. 11 CERTIFY 'FONDS ARE AVAILABLE : "." ' ,4 OSLIGAT 0 001 REFERENCE NO. CHARGE ALLOTMENT NO. CERT IF IED FOR PAYMENT OR CREDIT 'I 1 , 0' It01216 DATE, AUTHORIZED CERTIFYING OFFICER 1 SIGNATURE or A TANCEE � " 1,...� , I i . I SIGNATURE � � � � ' �, �, � ,, , ' ' � SPACE BELOW FOR EXCLUSIVE USE OF FINANCE 0 11,015 0 '�. __"it ......-4-,- 1 �..........., . *Evic*(0 ev . vq0toc***6: 7�12 ,e... 1 /if :' I. 1 ..,/1 . cc s.' v $4-30 - sr*T107-- , ICODE opoog�� 00100.� '[ _ MO 4�'42 ETPEISIS tc,spc 0 [ VI 43 -IF � 0 j! D � 4 A0,40 0,,Ay 1 IMP* tie� ,, 1 r" ' 47-52 - 0111.4�G.', REHN,13. sol.. :ATP:: � . � - � 414�`� ��Hom I' 4...1-4,,,,, Irer,. ..�, " CA TR ��- ,,-$4.. atideno4. ' 1'11�461 Acct.'. 111 sr . NO, . .'f , 3057 fe, ,,ri . ' Li.o.r. 0* IT' , *cc?. Imo.% '' 007O TIME .4.**TIt . 5100 v�-�� 71-SO AMOUNT, . " * `i v DESCR I PT I 010 ., � ADVANCE ACCOUNTS S3�17 � 101- 33 TfAso. �60,0m.0�� 1 P �05. Me* '''' � "'"*"'"Iiii ' ID, v 0 I II � 0!?41e7 cLAR. ASSIT 1 1 11 1 0 0 T.... : r � 1!1 01 t ..641 4,4A.'1...1- *..,.... I 9 601re'. 000 Ifirli II.4SIIIIIIIIII $ I I I 111111 I I I I -,� 1 ti I , /10�_.1. t � 1. e � iLf �; � i ril 4. 71 60 11�11 I I I I.5 I I 1 I 1 '0)1071;1101) 1 1c 1 ,rs� .10110.00 FORM' vet m4TooplicestITIONS. 100�00. .;".� - notified us that you hay re d A detai eta client of his grant fro We have prepared�the-detailed sateraen v c you re.! _ quire. . Iteaearch Accountant told se he-diseussed7thee ou-this'morning and it was. a You lioAlld again contant_th4 'contractor re the $18,20 unexpended balance reflected in the ..acesAinting ftf�-� the period ending August 31,- 1962, _not carried forward forward to the text grant year; � _ current grant period would be extendCtd. -tinte74nlyi.tc-30 June 1966, giving the grantee Jopportunie.. in the field the $123.69 the January 10th, 1966 --- letter. fro the original of of this et0-tr-it(4 s -nclos ,._ oe the February 10t 1966 letter from th which forwarded this 'grantee's. atcoun urea �uring the period - January ifkt-i-jpp3-4UOT-Igh�Ootiober 22nd, ,1963. . . _ , . s. auks Very!, such for /our help in the first ites. , , _ . . MEMCAXDUKirOK : Chief, TSDAB =MIXT :Dallnquent Accountings 0----MKULTRAi-SUbproject 143---- , 1. Eeview_of MKULTBA-p-Subproject 143 financial_records indicate that accOuntings for grants are now past due. It is_xequested-that your officeiinitiate action -to obtain the de3.in4pent_accounting8. 2. YOr_yow information fundi have been adVanced to the grantee U follows AuthOrization 1 $19,83&00 Grant period 1 Jan. - 31:Dec. 1963 ! AuthoriSation 2 i20,000.00 Grant periOd 1 Jan. - 31 Dec, I61 �-�"" 1 Distribution: Original-fe IMdressee - 4 � cluidwiedgediaf dated _ - "rt, � , - PROJECT CRYPTO :MKUMTRA-#143- _ GRANTEE PRINCrPAL INNESTIGATORI: DATE RENEWED :H=1 January 1964 During the past year, the investigators havestudied a total of 57 compounds for_their capacity to stimulate bacteria. These 57 compounds-consisted of 34 phenols, 14 amines, 5 alde- hydes and ketones, 3 quaternary ammonium compounds_and 2 un- classified chemicals. This stady has shown that_the_phenolic chemicals are_theiibest compounds for stimulating-bacteria. Of this group they_have-noted-that Q-phenyl--phenbl, p,itert-batyl- phenol and nonyl-phenol are the-bett compoundsistudied-so far. They havebeen_able to produce as much as_aJfour4old in- crease in microbial growth using these compounds, This degree of stimulation-is not as great as they had hoped it would be. _ _ For this reason,_during the past three weeks they_changed their line of approachito the problem. -- - They havepradaced_a product consisting of_phenol, casein hydrolyzate, yeast_extract and peptone which has_a_much-greater stimulating capacity, It appears that concentrations as small as 50 ppm will_give them a consistant 10 fold'increase-in-grow- th. The deterioration-of the cutting fluid-isgreater in three days than they-have ever observed in their studies-vith these products at anytime,In addition, as yet unpublished inform- ation from thislaboratory indicates that this-produce-should neutralize practically any known biocide that might be added to cutting flaids. It is belieVed"Nthat they have arrived at a point where dynamic progresscan be-anticipated. In view of_the_high-pro- - udcitivity and promise of resultS-that will be=of direct bene- fit to the operational aspects of DDP. It willTbe the purpose of the renewed project to combine-the two principles-of bact- erial stimulation(the-use of subeffective concentrations of Inhibitors and_the_use_of_non-specific protective compounds) to determine the_ultimate stimulation to be expected-in cut- ting fluids. More emphasis will be given to actual-degradative process a r se ban stimulation of growth per_se. In add- ition as agreed to investigate iiriai fouling of die e coo ng systems in conjunction with!his-other work. � . : . NAME OF DATE ' 30 Jartvz4ry 190g, AmouNT WO .00 FENNIEN NO. (Patience Use 001Y) REQUEST FOR ADVANCE $20_,000 CO OF FUNDS PAYABLE TO � Ir 0 0 �lea t 0 I' ROOM 1 eumoiNG TELEPNONE EXT. pporoNE� ,��:- AdVaTIC 0 to -...approvesi by 4 , � 7.,, Placa). Annex,sattacent � , . Pie ors ' or on 25 � 61;-- itunditglarMITA Subproject 143, invoice 4:# 2 mrhich activity January 1964. Accounting to be in accordance vitt% 111CULTRA B. 2 cLTLLjbru TM t,,, gil later than 10 Itebru 1 -T: vas 64 ! �� ..:��-� .. I . �,.- .',: 1 � � STATUS 0 I 1 OUTSTANDING 1 1 0 VA ES II 1 1 x siir to tits 1 unexpipaddid Chef i w if repartind plint ki,olaaci., iiyi....;,, stated 4 41 Authorise . r thie achrsece�Thy the dui die. cheeks deduction from my imagery submirsion 4 below te pi" vouchers in the efts* OWOOlt sett% and refund ot or fat t., t. 11111 0 any to 11 umexp o account I u . Wanes And INfund 1 1 1 1 DATE AMOUNT 1 UNACCONNIINDRSANCE I 0 I IRE OPTING POINT DOEII =11111 II 1111111111111til MINH 1 NANce osoosow ..1 HEADQUARTERS I I 11 1 h I I 111111111111 0 0 ARRIVAL i OR A801/F A ESTIOlirri 1 011 UI IIIIIIIMINIM11111111111 I 0 t 1 MORTHLY wORROAT � ON 'Or IlEACH THE LAST NORTH 1 VW Ill I 11111111111111111 � I Ill 1 , REQUESTING OFFICER APPROVED I RECEIPT FOR uNop 1 Aov AK II I 41 S GNATURE OF APPROVING 1 1 OFFICE* 11 1 I neknow 0.00 rece under to be used for se shown above. 0 the 1 weds purpose nithe dieted 11 1 se i 1 t accounted stetter: 1 .11 11 here. for 1 1 1 1 1� 4 1 r IV* :Qt ATE r AMOUNT I I ctieLIGATioat REFERENCE NO. CERT IF IED FOR PAYMENT OR CREDIT� 1 I � � '..� . ., RATE AUTHORIZED CERTIfYING OFFICER SIGNATURE OF ADVANCE E I 1DATE .; . , .10e^4 SIGNATURE � I: . j 1..H.VF ,_ SPACE BELOW FOR EXCLUSIVE USE OF FINANCE DIVISION . ,. REVIEWED 8Y . TOUCHER NO. 7.12 DESCAIPTION-ALI. OTHER et u 7' 18.33 34�311 0.42 4 6.a. 1 1:0,1,7; ,t::..11. CODE 47.52 881L11/3�. REF. CUP.. No, Noi. 1 CA V* 54-57 GENERAL ALLOT.' d ACT.IINO. 55. 57 OR COST SO. TO loNN I DATE 71- SO AMOUNTtAMOUNTI 1 11 � .DESCRIPTION--;;;;:;,' VAKE ACCOUNTS IA- 27 1 28-33 1 ,e�... � � � " ' � STATION CODE 1 1. 1' EXPEND, IcOcE 10. 0 ' 0 . H 1 oBJEC I CL AC I OlOIT I CP It It LEDGESc62-S ACCT NO. II 1 1 ..4.1...�1., x* p 11 i ,.., � � :* Qv. 1 1 0 MO.00CT NO. r :1 1.:1 II 1 1 1 1 1 1 1 1 1 'ikir-IlioteloiClot 1 1111 tillit � I 11111111 I 11 Eli I ii.oi-t000istlool ill'Ilii�tiltio of I swill 1�111 I I1111.1111110111 II I anI i1 . , IIII 11111111.0 11 lb al 1 :1��p".1 1 1 1 I 1 1 1 I 1 I 1 I'l I I 1 I I I 1 III I 1 1II 0 I 1 I 1 111 1111111 II 11 11111.1111.11,11111,111 1 'TOTALS , IIIIM11 fORM 60 �Loll USE POTV101.011 EDIY161111. SOVIEMOOKOT POINT.OtiOnt.cit 1142 ; rw.u. &January 1964 COVERT:ACTION-SUPPORT B ! --Sabotage . Branch B Category IVa roject Title _Item Cla-fication ied (Data) _ Task No Type of Contra. DateIrdMiateci ,21_January_1964 TT $2 CcO2M4 $20 RO 0 Completion Date - Purpose: The objedtive of the first year was acnieved._:An approach- o the stimulati6h_of soluble-oil degradation vas-proved feasible. Iin- addition a system for negating microbial inhibitors was developed. These two accomplishments appear Wbe the firstsignificant break- through for mitrobialidegradation of materials. It is planned-to---- study furtberthelliditatiens of-th-etia-approAches and-theit-applicatiol to a variety7of-praCtiCal-bituations during the coming year. - Status: Performance has been excellent. Has lrogressed beyond-expect- ations. Current in fiscal matters, - Requirement: �Generated by undocumented requests_from DP units and div- isions as well As necessity for TSD-t0 maintain_a_quick delivery cap- ability to meet anticipated future operational needs. 4. The cost of this program for the second year is $20,000.00. To this sum must be added $800.00 representing a four percent service-charge to -the' The ill function as cut-out and 0-thisi-grant--The cover title of the_project_is _ . Total cost 61-the project will hotexceed-$20,800;0 Charges shOtild be made agaitst7Allotment No.--4125;=.1aga.=3902. 6. Ill request th to submit :a summary-accounting of mohies-receiVed and to return any_unexpended grant funds at-the end_of tht litoject year. 7. Title to any permanent equipment purchased by funds granted th shall be retained by7thet-in-lieu of higher overhead rates. - 8. It was mutually agreed that documentation and acc- ounting for-travel expenses which are reimbursablt-hY the hall conform with the acceptedipractites of _ that institUtion. Attachments:- Pr op o_sal.ik- budget re Vint 4r:s Distribution: Original-only el- a CO7-44 ;* a 11 � 4 1 y� r-777777JW7171. T MEMORANDUM-1'0R THE RECORD SUBJECT' :--MKULTRAI Subproject 143 143 is to-continue support 1. The_purpost-of subpro of the work:Of: on an approach-to microbial t deteriorationof_materialS, 2. Intringthe -first year a'syStem-was.develOed whiCh allows A 10_ftild-Stimulation of the microbial deterioration -of solubleoilltoilants (as:4 type system) usinvextremelr7-7:7-- small qUantities-:_of.actiVe material, In additian a system - was found:which_appartntly_serves to inactivate-most-micro-- bial inhibitors in-suc 'a product. This wasAuuch-more-of an accomplistiment_than was-expected. For the coming year-it is planned to-explore-the limitations of-these approaches as _ _ _ they apply-tocutting=oils and other petroleum based products. It is hoped that these techniques will T?e a_pplicableacross- the-boarcLtoiall_sabotage or harass rent systems involving microorganisms, promoting a more-deIlverable package-to_the operator.' 3. principal investigator;_has-been- cleared through SECRET. _AMMIM " � � � ii : 5.u3.!�:-..4.1.a..N.:.Y73--,I Fort SUMECT- -L-mxt.L.TRA --Subp,:ojee . _ a uary 964 Under the : au t bori ty-- gr atdth t _cle:=2oranduridat.o.d_13:-A-nril- 1953 .. . . from th rcr to th-a-DO/A , and the edraenclon o.f th -aa,.1tho-r. -I vs; 1 1.1 filth- � 4.equer,t � rzi.tmoranda, ----143 hashtr -of tne ubproject4a e - � APPROVED OF FlItelD Date: Pros: MKULTAT-fur 3 .7...a vs _ ATION- _ DistributIcn: �rig Z1 1 - 2 - BB GROUP_T�.. . fn)m auto .;�7:: 3 � � :ill � 72' - As you know, the grant :from, one year old and itAs_tiMelt6 1 During the past year wellave_studied a-total of 57 comounda=for-their capacity to stimnate_bacteria. These_57 compounds consiSted_or 34 � phenols, 13 amines, 5 aldehydes and ketones, 3 quaternary-ammonium-- compounds and 2 tnclassifiediCheiicals�This:tudy_has_shown-that-the phenolic chemicalsiare-the_hest_compounds_for_stimulatinEbetteria.- Of this group we haveinoted-that Qphenyl-phenol, p-test-batylphenol, di- sec-butylphenol,:dbdecyl pehnol, 214-dimethyl--6-test-butylphenoand� nonyl phenol are-the_best coovounds_studied_so_far_._. We have been ableAoiprodUce as-much_as_a fourfold_increase-inFMiorobial - growth usinuthetecompounds. This degree of stimulation-ia-tiOtTas- ----- great as we had hoped-it7Wciuld-bo.__For_this_reason� during-the-past three weeks we changed our line of_approach to the problem; We have produced aL-product_consisting of phenol, casein, hydrolyzate, yeast extract and_paptone_whichlhas a much_greater stimulating capacity. It appears that eoncentrationt_as_small_as_5() ppm will giveus_a_consis- tent 10 fold increaseAn=groWth._ The_deteriorationlof_the-cutting fluid is greater in threedays_thanTwe have ever observed in our-studies_with- - these products at any-time.- In addition, as yet umpublished_infermation from this laboratory indiCates that this product should neutralize prac- tically any knowA_bibcideLorLstat_that might,be_added_to:cutting fluids. We feel that we how haveferritediata_point where_we can_makeisomeAYnamic progress. During th coming year we hope to: : 1. Study additional chemicals and_media_products_for_theirstiMulatory capacity. 2. Determine the best_ratios of_the media_components_and generally__ _ improve the fOrmulition. 3. Determine the effect of the formulation_on_other_petroleum_produots.- 4. Determine if the:formulation_has the_capacity_to_neutralizeanti- microbial agents. S. Begin work on how the_formulition_stimulates microorganisms. 6. Determine the effect of the formulation plus urine_on microbial-growt I believe these points cover the main objectives for the coming-year. Kindest personal regards. Sincerely yours,: PROPOSED_OPAIT_COST Prineipalinvestigator_( time) 3990.00 Full time-research _assistants 9417.00 B� Expendal1e_eq4pment-_ata supplies 2560.00 C. Typing reports; papers, etc. 700.00 D� Overhead (20%) 3333.00 sicirAL 20,000-00 - 27 January_1964 MEMORANDUM-FOR_TRE RECORD SUBJECT: MKULTRA_Subproject_143 . At the request of both Chief and_Deputy_Chief, TS wai-contactedifor the purpose of funding this MKULTRA u hrau h one of the established funding mechanisms controlled by Due primarily to the futiaing date, it was_riot_possible - to accomplish this at this time. 2. Arraigements illkemadewit to project our funding requirements_fcTr MKULTR Zu� p ojects on a serni-annu-al basis. With thisAea of funding our total_re_quirernents. 3. �11 meet with funding echamsm _at_1115 this_date: feels that they_may ca.pable o discuss other_Subprojects_ ExeMd v!?,713111, triax'2!..; - - ' . COVERT ACTION SUPPORT Branch._ BB Category: SAbotage - (formerly :IIIb)_ iProject Engineer Task No e of Contract - ---- Purpose: 'To 'investigate the poter.tial of a new approach- to- the-stiir. ulation--Of raicrobia-l=deterioration-of petroleum 151"odutI and its possible-application to other. degredative Crocesse . _ _ . Status: Contractor has-develooed-a. model system for desired system. Will now attempt-to toiis'nftl scle uriwt fiscalatters. - � � Requirement: Generated by-undocumented requests from DDP units-and divisions as well_as.necessity for TSD to maintain a quick delivery capability-to meet anticip-ated-future operational needs. - a � 7: ' .- BECELE'T DRAFT__ 11 December 1962 on an approach to the stimulationiotpetroleum _product deterioration. 2. Although the-tOdel system for these studiee_vill-be_pet- roleum productsi-thia_appreoeh to stitulation of deterioration may have vide-application to all areas of biologicar,sabbtage-currently , of interest ito TSD. 3. principal investigatrjM3beencl�. through SECRET, 4. The cost of this program for the first year is $19,636.00. To this sum-must be-tddid $793.44 representing a:rour_per cent service_ functiona!vsut out and cover f6f-this grant. The-cover-title Of the 5. Total_cost_of-the project for a period-of one-year will -tot exceed $20,62944.' Charges should be made againstJAllotmen't No. # 11 request th a summary acCbunting-of monies received and to returnLany-nex- - - _ _ _ ;ended grant funds at the end of the project year. Title to any permaneni:ewipment purchased by fUndalra the hall be retained by theiln lieu ofbieler over- head rates. . It iossmutually agreed-that7documentation WI-accounting__ for travel expenseevbilch are reimbursable by Cceformvith the_accepted practices of that-institution. 9. The requirement for a six months' informal accounting on_ the part of the7principal investigatbr is waived. hall Attachment: Proposal &iudge t Distribution: Original only � "Tr TSD iological Branch 1,--1 c�.(117 p arbdrg- d can place the following equipment a th-ediposa -Ultra-centrifuge --* Ratheon: 'Son i 6- Osc i I --Mickie Disintegrator Centrifuges-(refrigerated, Sorv� International, Ultra-centrifuge, Si4inco, etc.) 4 _ _ _ Sharpl esSUper Centr I fuge:' -BeckMan 0141-60 Spectrophotometers � Beckman 8 Incubators_ Research pH Meters Shaking Incubators Kjeldahl Apparatus Colorimeters Pilot Plant Fermentors Chromatography Equipment' Fraction coil ectors . Isotope laboratory with-complete supporting�equipment - To determine�If_the _growth of microorganisms_in petroleum products can be markedly increased by the addition of substatic concentrations of antimicrobial agenis._, ITEWANKEll_IEE as been ca_refuLLy searched b_ deterpine_what microbial, growti relate to thitopic,, mshen`ei-iiii and PeroVa institute_of Microbiology, Academy o � _ -� - �. ��-- � � cierice U.S:S. ikrobiologiya', 23. 53-56, 1954) found that Cultures_ of SaccharOmyces:ceeviTiae�itr_ained to grow In of - , � . .,--- small concentrations pUligC12;,orphenols have much higher _fermentation:, ,,,,�-�._%�� '-'..--;:i0.:::;:�"4 ;:'_1�:'-�: . ti:L-.. - 4,-. ,,, '-.,.-:. 1.-:-�; '''''' ..:' `. � f-r� ': : ". _ , 7 ", � .:, �:, - .":�:' _ : . , �,.. . ' - ' ' rates r.than..,culturesthat_have_not_been_trained to_grow_in_the...presence�� :As ,iittre_as_50_ ppm of HgCli accelerates 7 the_. oworkers believe_thatthls effect is to traces of inhiUtor_in_the environment .and_not_because_of some change in the physiology of the ' cells reported that 50 ppm of-2 richloro phenol CouLd_rnarkedly stimulate the growth of bacteria in-acraision_oils. in some instances this produceslmost a'three fold increase in the number of ceLl s in the environment, found that .25 to' 150 ppm of--2,4-dinitrophenol increases the oxidation � of Cie), _C12, C13, C14, and c6fatty acids. The 1;�f, � increase was asinuch-as 100% and was not associated with an 'increase in � _ t tnthe environment These results imply that two types of stimulation may be - . _ produced by subtatic concentrations-of inhibitors. First, a metabolic stimulation-ma/ be produced where the cells -do not increase in number but thisimetabotc rate is�increased. Second, the number . . . of cells in the environment may e increased by the-compouncl. It '- remains to be see_Cy'l-whetlier. there I a stimulation-in-the individua ' metabolic activity ofeach cell in this case. -.There is an increase _ in activity, however,--ihis may only be due to the_�reater-ntimbir-of , _ have found that - � ro- Vut-y1 -acetate produce as 3 fciid increase in duct ion oLpeniciltiifbii�ium chrysogenum. PLAN OF ATTACK. is well known -that the -rate of oxidation of hydrocarbons ma be limited by the rate of_d-C.H. fi-Sion of the compound across the - - _ � - � � permeability barrrer It is also well known that -surface active agents increase the permeability of--the cytoplasmic membrane. Several-years ago developed the idea that the use of surface active compounds may increase the accessibility of hydrocarbon substrates to microbial attack and thus' speed.up the oxidation of these compounds. Due to several factors including a lack of funds, this idea has � never been tested to any extent. To date, we have studied 3 phenol id compounds. Two of these compounds produced-stityulation and one did not. We propose_to_study the effect of substatic concentrations o � atty-amines t:Oh-031s, and 06 t-epill'y an nlurcornpourids on . - � 7the oxidation of_two different synthetic coolantsi.andone petroleum: type-prodUct..: may also use Other antimicrobi_alegents other :than those -1is_te4==abOve. -. microorganisms belonging :te Genus used in this investigation.i.- The cultures ri samples Of used cutting fluids _obtained MEDIA Whenever possible, the organisms will be waratatned in cutting � luids In SoWinstanCeS cony ntitinar Midi-6-$uCh_aiJmitrient'brath,. _ meat -infuiionrbicith or'en-inor-ganic-sal $ medium7may-be used , INCUBATION TEMPERATURE _ All experiments wit] be done at 30�C The length of the incubation perib0 Will vary according to the organiMend'the particular experiment. CHEMICALS - - The-best grade of chemical obtainable-will be used throughout this investigatiOn- GLASSWARE All flasks, tubes and other-glasS-eOuipment will be actd cleaned prior to each experiment. HYDROGEN ION CORCENTRATION _ The pH of the environment will-vary according to the _particular experiment. In general, we will let-theFPh of the cutting Mid 'Le determine the pH 'of the INpcut.um :Thercells-used_in_the experimentS will be grown on an-appropriate _ . medium. They will_be_harvested_after_a suitable incubation_period _ nd wasfle('..1 thretmes1n_iso.ionia_sarine. The cells_w_1111 be suspended in is�n!csa1ine--, quantities undorm- lithissuspens ion will be used 'tc:Linoctilate the tubes or. flasks STERILITY'f . We will employ suitable procedures for sterilizing the media - - Appropriate_controls Will_be included An each eXpertment. Lack experiment will�be_done_inricate and repeated_at least once CUTTING FLUIDS. Three different cutting iLuids will be used in-thts-investigation -.- consisting of'two_chemicar coolants and one petroleum product. .EXPERIMENTAL TECHMQUE Each cuttinj-ftuid will beIprepared in a II =25-d1lution in tap water., Ore huriclred_ml_othis_coolant will he placed in each 250 ml Erlenmeyer flask. The number oflasks_used_ in each experiment will vary from time to time. T_h_e_i_n_hibitor_will_be added to the series of flasks in vary ing-conceramt tons. these f_Lasks_cli I lb�rLli� autoclaving and then inoculated with the test orgartiSms. The flasks _ will be incubated for a period of time (probably two_weeks) in a shaking incubator. At periodic -Untervals of_about 2 days time the number of cells per ml will be-determined for each flask. At the same-time the pH of the flask will be_determined and other biochenlical tests_(such as dehydrogenase' activity) may be conducted to determine the degree of m.tAhnItn e'etti.:/itv_th_the_environment- At the _ehd-of the exoertment ' the. miaining cuttIng_fluid will Iii�poure :into a_vOlumetrIc cyl inder to determine- thequanti,ty of free oil in tfle- product (this test will be used on the petroleum coolant only). The quantity of free oil present is ars7_-Cel lent index of the degree of_attack_on_the emulsifier.: We will study as many compound's as -possible during the year period of this grant. In no case will�thRibe less than twenty- five: compounds caST:OF�PROJEeT - PriAcipal Investigator $200.00/month (9 months) Full time salary to work on project in , ummer_- 575imonth each Publication Cost _ Typing- report, papers, and -Ourchasihg-reprints , - of publications that prise from this: work r . .LC0 001 $. 19,836.00

Printer-friendly version

Search form

MKULTRA SUBPROJECT 143 (23 DOCUMENTS WITH PUBDATES FROM 621211 TO 6602 23)